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7.116667 0 Td 50.87963 Tz (Degeneration-related ) Tj 6.358333 0 Td 58.333333 Tz (and ) Tj 1.302778 0 Td 54.398148 Tz (trauma-induced ) Tj 4.822222 0 Td 63.194444 Tz (damage ) Tj 2.508333 0 Td 46.990741 Tz (to ) Tj 0.836111 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 753.274093 Tm 6.377778 0 Td 46.990741 Tz (spinal ) Tj 1.963889 0 Td 47.314815 Tz (discs ) Tj 1.633333 0 Td 50.771605 Tz (are ) Tj 1.147222 0 Td 48.251029 Tz (currently ) Tj 2.819444 0 Td 47.685185 Tz (treated ) Tj 2.236111 0 Td 52.662037 Tz (with ) Tj 1.516667 0 Td 41.049383 Tz (cells, ) Tj 1.691667 0 Td 57.253086 Tz (growth ) Tj 2.313889 0 Td 43.75 Tz (factors, ) Tj 51.428571 0 0 51.428571 0 710.469182 Tm 6.358333 0 Td 47.314815 Tz (injectable ) Tj 3.111111 0 Td 46.990741 Tz (materials, ) Tj 3.091667 0 Td 54.282407 Tz (such ) Tj 1.555556 0 Td 51.851852 Tz (as ) Tj 0.894444 0 Td 47.395833 Tz (silicone ) Tj 2.508333 0 Td 44.722222 Tz (fluid ) Tj 1.594444 0 Td 53.472222 Tz (or ) Tj 0.855556 0 Td 49.907407 Tz (elastomer, ) Tj 3.266667 0 Td 51.851852 Tz (or ) Tj 51.428571 0 0 51.428571 0 671.639594 Tm 6.377778 0 Td 46.990741 Tz (Surgical ) Tj 2.547222 0 Td 53.148148 Tz (procedures ) Tj 3.519444 0 Td 54.282407 Tz (such ) Tj 1.594444 0 Td 50.231481 Tz (as ) Tj 0.875 0 Td 46.990741 Tz (tissue ) Tj 2.002778 0 Td 55.092593 Tz (removal ) Tj 2.644444 0 Td 43.75 Tz (in ) Tj 0.855556 0 Td 52.146465 Tz (conjunction ) Tj 51.428571 0 0 51.428571 0 628.09201 Tm 6.338889 0 Td 54.282407 Tz (with ) Tj 1.477778 0 Td 53.472222 Tz (or ) Tj 0.797222 0 Td 52.777778 Tz (without ) Tj 2.372222 0 Td 47.530864 Tz (spinal ) Tj 1.905556 0 Td 51.851852 Tz (implants ) Tj 2.683333 0 Td 57.685185 Tz (which ) Tj 1.905556 0 Td 49.907407 Tz (often ) Tj 1.672222 0 Td 54.012346 Tz (induce ) Tj 2.138889 0 Td 46.8107 Tz (vertebral ) Tj 51.428571 0 0 51.428571 0 588.420048 Tm 6.358333 0 Td 46.759259 Tz (fusion. ) Tj 2.138889 0 Td 49.583333 Tz (Generally, ) Tj 3.169444 0 Td 47.530864 Tz (repair ) Tj 1.827778 0 Td 55.092593 Tz (of ) Tj 0.719444 0 Td 62.962963 Tz (damaged ) Tj 2.780556 0 Td 48.611111 Tz (disc ) Tj 1.302778 0 Td 50.231481 Tz (as ) Tj 0.777778 0 Td 48.287037 Tz (functional ) Tj 3.091667 0 Td 46.450617 Tz (living ) Tj 51.428571 0 0 51.428571 0 543.941846 Tm 6.338889 0 Td 46.990741 Tz (tissue ) Tj 1.944444 0 Td 38.888889 Tz (is ) Tj 0.719444 0 Td 40.509259 Tz (difficult. ) Tj 2.683333 0 Td 64.814815 Tz (The ) Tj 1.4 0 Td 54.444444 Tz (cause ) Tj 1.866667 0 Td 59.953704 Tz (of ) Tj 0.855556 0 Td 48.611111 Tz (disc ) Tj 1.419444 0 Td 51.851852 Tz (degeneration ) Tj 4.005556 0 Td 52.932099 Tz (has ) Tj 1.205556 0 Td 59.143519 Tz (been ) Tj 51.428571 0 0 51.428571 0 504.253731 Tm 6.377778 0 Td 50.87963 Tz (speculated ) Tj 3.247222 0 Td 45.37037 Tz (to ) Tj 0.758333 0 Td 58.333333 Tz (be ) Tj 0.855556 0 Td 58.333333 Tz (a ) Tj 0.544444 0 Td 46.8107 Tz (resultant ) Tj 2.702778 0 Td 56.712963 Tz (of ) Tj 0.777778 0 Td 57.523148 Tz (poor ) Tj 1.555556 0 Td 47.800926 Tz (disc ) Tj 1.4 0 Td 46.585648 Tz (nutrient ) Tj 2.430556 0 Td 53.472222 Tz (Supply ) Tj 2.119444 0 Td 60.493827 Tz (and ) Tj 51.428571 0 0 51.428571 0 460.39816 Tm 6.338889 0 Td 55.740741 Tz (waste ) Tj 1.963889 0 Td 52.256944 Tz (removal. ) Tj 2.838889 0 Td 62.654321 Tz (The ) Tj 1.419444 0 Td 50.555556 Tz (inner ) Tj 1.827778 0 Td 51.851852 Tz (nucleus ) Tj 2.488889 0 Td 53.877315 Tz (pulposus ) Tj 2.916667 0 Td 56.712963 Tz (of ) Tj 0.855556 0 Td 51.851852 Tz (the ) Tj 1.244444 0 Td 46.180556 Tz (disc ) Tj 1.438889 0 Td 40.509259 Tz (is ) Tj 51.428571 0 0 51.428571 0 420.733417 Tm 6.358333 0 Td 47.685185 Tz (largely ) Tj 2.313889 0 Td 52.932099 Tz (nourished ) Tj 3.130556 0 Td 58.333333 Tz (and ) Tj 1.341667 0 Td 47.800926 Tz (relieved ) Tj 2.566667 0 Td 58.333333 Tz (of ) Tj 0.913889 0 Td 50.378788 Tz (metabolites ) Tj 3.597222 0 Td 59.953704 Tz (by ) Tj 1.030556 0 Td 47.890947 Tz (transport ) Tj 51.428571 0 0 51.428571 0 379.417186 Tm 6.338889 0 Td 54.166667 Tz (through ) Tj 2.488889 0 Td 49.691358 Tz (the ) Tj 1.088889 0 Td 56.388889 Tz (upper ) Tj 1.847222 0 Td 60.493827 Tz (and ) Tj 1.283333 0 Td 55.740741 Tz (lower ) Tj 1.866667 0 Td 47.170782 Tz (vertebral ) Tj 2.761111 0 Td 50.05144 Tz (endplates ) Tj 2.955556 0 Td 58.333333 Tz (and ) Tj 1.263889 0 Td 44.560185 Tz (less ) Tj 1.302778 0 Td 51.851852 Tz (so ) Tj 51.428571 0 0 51.428571 0 336.17 Tm 6.338889 0 Td 54.166667 Tz (through ) Tj 2.566667 0 Td 51.851852 Tz (the ) Tj 1.225 0 Td 53.703704 Tz (annulus ) Tj 2.586111 0 Td 45.37037 Tz (fibrosus. ) Tj 2.761111 0 Td 52.314815 Tz (Various ) Tj 2.547222 0 Td 54.166667 Tz (medical ) Tj 2.605556 0 Td 48.021886 Tz (conditions, ) Tj 51.428571 0 0 51.428571 0 295.596226 Tm 6.377778 0 Td 54.282407 Tz (Such ) Tj 1.536111 0 Td 51.851852 Tz (as ) Tj 0.894444 0 Td 44.398148 Tz (Sclerosis, ) Tj 2.916667 0 Td 62.654321 Tz (damage ) Tj 2.469444 0 Td 50.771605 Tz (the ) Tj 1.186111 0 Td 46.8107 Tz (vertebral ) Tj 2.761111 0 Td 51.131687 Tz (endplates ) Tj 3.013889 0 Td 58.333333 Tz (and ) Tj 1.322222 0 Td 45.37037 Tz (limit ) Tj 51.428571 0 0 51.428571 0 252.345133 Tm 6.358333 0 Td 48.251029 Tz (diffusion ) Tj 2.819444 0 Td 46.990741 Tz (to ) Tj 0.758333 0 Td 59.41358 Tz (and ) Tj 1.283333 0 Td 57.523148 Tz (from ) Tj 1.613889 0 Td 50.771605 Tz (the ) Tj 1.088889 0 Td 44.722222 Tz (disc. ) Tj 1.536111 0 Td 54.282407 Tz (This ) Tj 1.536111 0 Td 51.041667 Tz (lack ) Tj 1.380556 0 Td 59.953704 Tz (of ) Tj 0.855556 0 Td 47.890947 Tz (transport ) Tj 2.761111 0 Td 46.990741 Tz (to ) Tj 0.758333 0 Td 61.574074 Tz (and ) Tj 51.428571 0 0 51.428571 0 214.893773 Tm 6.358333 0 Td 58.333333 Tz (from ) Tj 1.633333 0 Td 49.691358 Tz (the ) Tj 1.127778 0 Td 46.990741 Tz (disc ) Tj 1.361111 0 Td 45.37037 Tz (likely ) Tj 1.866667 0 Td 57.407407 Tz (impedes ) Tj 2.625 0 Td 50.925926 Tz (present ) Tj 2.333333 0 Td 49.421296 Tz (regenerative ) Tj 3.772222 0 Td 51.131687 Tz (treatment ) Tj 51.428571 0 0 51.428571 0 172.466907 Tm 6.377778 0 Td 54.166667 Tz (systems ) Tj 2.45 0 Td 45.37037 Tz (in ) Tj 0.680556 0 Td 51.851852 Tz (the ) Tj 1.088889 0 Td 47.530864 Tz (repair ) Tj 1.847222 0 Td 55.092593 Tz (of ) Tj 0.719444 0 Td 62.962963 Tz (damaged ) Tj 2.8 0 Td 44.830247 Tz (discs. ) Tj 1.769444 0 Td 53.472222 Tz (This ) Tj 1.458333 0 Td 50 Tz (disease ) Tj 2.255556 0 Td 49.826389 Tz (etiology ) Tj 30 0 0 30 0 2547 Tm 41.666667 0 Td 75 Tz (10 ) Tj 30 0 0 30 0 2339 Tm 41.666667 0 Td 72.222222 Tz (15 ) Tj 30 0 0 30 0 1923 Tm 41.6 0 Td 77.777778 Tz (25 ) Tj 30 0 0 30 0 1716 Tm 41.6 0 Td 80.555556 Tz (30 ) Tj 30 0 0 30 0 1507 Tm 41.6 0 Td 77.777778 Tz (35 ) Tj 30 0 0 30 0 1299 Tm 41.566667 0 Td 83.333333 Tz (40 ) Tj 30 0 0 30 0 1091 Tm 41.566667 0 Td 80.555556 Tz (45 ) Tj 30 0 0 30 0 883 Tm 41.6 0 Td 80.555556 Tz (50 ) Tj 30 0 0 30 0 675 Tm 41.6 0 Td 77.777778 Tz (55 ) Tj 30 0 0 30 0 467 Tm 41.633333 0 Td 77.777778 Tz (60 ) Tj 30 0 0 30 0 259 Tm 41.633333 0 Td 75 Tz (65 ) Tj 51.428571 0 0 51.428571 0 2969 Tm 33.775 0 Td 74.537037 Tz (2 ) Tj 51.428571 0 0 51.428571 0 2916.878453 Tm 25.297222 0 Td 38.888889 Tz (is ) Tj 0.875 0 Td 48.287037 Tz (paralleled ) Tj 3.325 0 Td 43.75 Tz (in ) Tj 0.952778 0 Td 51.203704 Tz (other ) Tj 1.886111 0 Td 45.37037 Tz (joints ) Tj 2.061111 0 Td 50.231481 Tz (as ) Tj 1.05 0 Td 46.018519 Tz (well. ) Tj 1.847222 0 Td 50.771605 Tz (Diffusion ) Tj 3.169444 0 Td 47.800926 Tz (limiting ) Tj 51.428571 0 0 51.428571 0 2873.169306 Tm 25.297222 0 Td 55.555556 Tz (changes ) Tj 2.547222 0 Td 45.37037 Tz (in ) Tj 0.758333 0 Td 50.771605 Tz (the ) Tj 1.108333 0 Td 45.730453 Tz (calcified ) Tj 2.644444 0 Td 60.493827 Tz (and ) Tj 1.302778 0 Td 50.231481 Tz (vascular ) Tj 2.566667 0 Td 46.296296 Tz (tissues ) Tj 2.177778 0 Td 58.333333 Tz (of ) Tj 0.797222 0 Td 53.914141 Tz (Subchondral ) Tj 51.428571 0 0 51.428571 0 2831.809868 Tm 25.297222 0 Td 58.333333 Tz (and ) Tj 1.225 0 Td 51.203704 Tz (cancellous ) Tj 3.266667 0 Td 59.953704 Tz (bone ) Tj 1.594444 0 Td 54.166667 Tz (precede ) Tj 2.45 0 Td 49.691358 Tz (the ) Tj 1.069444 0 Td 51.203704 Tz (onset ) Tj 1.730556 0 Td 56.712963 Tz (of ) Tj 0.777778 0 Td 45.601852 Tz (osteoarthritic ) Tj 3.986111 0 Td 46.018519 Tz (joint ) Tj 51.428571 0 0 51.428571 0 2789.382716 Tm 25.297222 0 Td 45.37037 Tz (failure ) Tj 2.1 0 Td 42.12963 Tz (in ) Tj 0.719444 0 Td 46.990741 Tz (diarthrodial ) Tj 3.538889 0 Td 45.37037 Tz (joints ) Tj 1.827778 0 Td 51.851852 Tz (as ) Tj 0.797222 0 Td 46.666667 Tz (well. ) Tj 51.428571 0 0 51.428571 0 2749.600249 Tm 26.016667 0 Td 47.685185 Tz (Osteoarthritic ) Tj 4.219444 0 Td 51.311728 Tz (degenerative ) Tj 3.869444 0 Td 46.018519 Tz (joint ) Tj 1.594444 0 Td 50 Tz (disease ) Tj 2.294444 0 Td 59.41358 Tz (and ) Tj 1.283333 0 Td 48.971193 Tz (disorders ) Tj 2.858333 0 Td 59.953704 Tz (of ) Tj 51.428571 0 0 51.428571 0 2705.919897 Tm 25.277778 0 Td 50.771605 Tz (the ) Tj 1.225 0 Td 48.070988 Tz (spinal ) Tj 2.061111 0 Td 59.953704 Tz (column ) Tj 2.469444 0 Td 46.990741 Tz (\(discs ) Tj 1.983333 0 Td 60.493827 Tz (and ) Tj 1.4 0 Td 48.611111 Tz (vertebrae\) ) Tj 3.247222 0 Td 54.73251 Tz (accounted ) Tj 3.247222 0 Td 48.611111 Tz (for ) Tj 1.147222 0 Td 59.953704 Tz (an ) Tj 51.428571 0 0 51.428571 0 2666.979444 Tm 25.297222 0 Td 51.851852 Tz (estimated ) Tj 2.975 0 Td 56.944444 Tz (815,000 ) Tj 2.547222 0 Td 60.493827 Tz (and ) Tj 1.322222 0 Td 56.018519 Tz (634,000 ) Tj 2.527778 0 Td 74.537037 Tz (US ) Tj 1.147222 0 Td 52.572016 Tz (diagnoses ) Tj 3.033333 0 Td 46.990741 Tz (in ) Tj 0.777778 0 Td 52.5 Tz (discharged ) Tj 51.428571 0 0 51.428571 0 2626.046512 Tm 25.297222 0 Td 45.37037 Tz (inpatients, ) Tj 3.15 0 Td 45.868946 Tz (respectively, ) Tj 3.733333 0 Td 52.391975 Tz (during ) Tj 1.963889 0 Td 54.444444 Tz (2007. ) Tj 1.75 0 Td 58.333333 Tz (These ) Tj 1.847222 0 Td 52.441077 Tz (occurrences ) Tj 3.616667 0 Td 59.143519 Tz (were ) Tj 51.428571 0 0 51.428571 0 2585.595122 Tm 25.297222 0 Td 55.092593 Tz (ranked ) Tj 2.197222 0 Td 48.611111 Tz (fourth ) Tj 1.944444 0 Td 59.41358 Tz (and ) Tj 1.302778 0 Td 48.611111 Tz (ninth ) Tj 1.691667 0 Td 43.75 Tz (in ) Tj 0.758333 0 Td 49.789562 Tz (prevalence, ) Tj 3.5 0 Td 52.932099 Tz (excluding ) Tj 3.052778 0 Td 55.812757 Tz (pregnancy ) Tj 48.571429 0 0 48.571429 0 2541 Tm 26.785294 0 Td 61.764706 Tz (and ) Tj 1.379412 0 Td 49.754902 Tz (childbirth ) Tj 3.273529 0 Td 49.509804 Tz (related ) Tj 2.347059 0 Td 50.098039 Tz (instances, ) Tj 3.252941 0 Td 64.052288 Tz (and ) Tj 1.420588 0 Td 50.612745 Tz (resulted ) Tj 2.697059 0 Td 48.039216 Tz (in ) Tj 0.844118 0 Td 61.764706 Tz (a ) Tj 0.638235 0 Td 48.801743 Tz (financial ) Tj 52.857143 0 0 52.857143 0 2500.880893 Tm 24.594595 0 Td 54.129129 Tz (burden ) Tj 2.251351 0 Td 58.333333 Tz (of ) Tj 0.908108 0 Td 52.342342 Tz ($20.2 ) Tj 1.872973 0 Td 43.243243 Tz (billion ) Tj 2.156757 0 Td 45.305832 Tz (\(inflation-adjusted ) Tj 5.467568 0 Td 46.509009 Tz (hospital ) Tj 2.516216 0 Td 43.243243 Tz (costs\). ) Tj 51.428571 0 0 51.428571 0 2458.38822 Tm 25.316667 0 Td 50.555556 Tz (Levit ) Tj 1.788889 0 Td 81.018519 Tz (K ) Tj 0.738889 0 Td 63.194444 Tz (W. ) Tj 1.05 0 Td 48.611111 Tz (L. ) Tj 0.894444 0 Td 49.331276 Tz (Stranges, ) Tj 2.975 0 Td 43.75 Tz (E. ) Tj 0.855556 0 Td 55.740741 Tz (Ryan, ) Tj 1.983333 0 Td 84.259259 Tz (K ) Tj 0.797222 0 Td 48.316498 Tz (Elixhauser, ) Tj 3.422222 0 Td 38.888889 Tz (A., ) Tj 1.030556 0 Td 80.208333 Tz (HCUP ) Tj 51.428571 0 0 51.428571 0 2417.470366 Tm 25.316667 0 Td 50.555556 Tz (Facts ) Tj 1.75 0 Td 62.654321 Tz (and ) Tj 1.361111 0 Td 46.990741 Tz (Figures. ) Tj 2.586111 0 Td 43.425926 Tz (Statistics ) Tj 2.858333 0 Td 58.333333 Tz (on ) Tj 0.991667 0 Td 52.777778 Tz (Hospital-based ) Tj 4.627778 0 Td 57.523148 Tz (Care ) Tj 1.613889 0 Td 45.37037 Tz (in ) Tj 0.797222 0 Td 47.530864 Tz (the ) Tj 51.428571 0 0 51.428571 0 2374.124694 Tm 25.355556 0 Td 54.552469 Tz (United ) Tj 2.119444 0 Td 44.907407 Tz (States, ) Tj 2.119444 0 Td 53.796296 Tz (2007. ) Tj 1.847222 0 Td 54.444444 Tz (2009, ) Tj 1.788889 0 Td 62.114198 Tz (Agency ) Tj 2.488889 0 Td 47.530864 Tz (for ) Tj 1.069444 0 Td 51.851852 Tz (Healthcare ) Tj 3.363889 0 Td 54.282407 Tz (Research ) Tj 51.428571 0 0 51.428571 0 2334.088058 Tm 25.297222 0 Td 58.333333 Tz (and ) Tj 1.205556 0 Td 49.016204 Tz (Quality: ) Tj 2.605556 0 Td 49.259259 Tz (Rockville, ) Tj 3.130556 0 Td 64.814815 Tz (Md. ) Tj 1.380556 0 Td 53.742284 Tz (Degeneration ) Tj 4.063889 0 Td 37.268519 Tz (is ) Tj 0.622222 0 Td 49.907407 Tz (associated ) Tj 3.188889 0 Td 53.472222 Tz (with ) Tj 51.428571 0 0 51.428571 0 2293.26747 Tm 25.297222 0 Td 56.712963 Tz (an ) Tj 0.855556 0 Td 57.79321 Tz (overwhelming ) Tj 4.336111 0 Td 50.636574 Tz (majority ) Tj 2.586111 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 49.907407 Tz (these ) Tj 1.652778 0 Td 47.530864 Tz (cases, ) Tj 1.847222 0 Td 59.41358 Tz (and ) Tj 1.205556 0 Td 50.231481 Tz (as ) Tj 0.777778 0 Td 54.282407 Tz (such ) Tj 1.438889 0 Td 55.632716 Tz (arthro ) Tj 51.428571 0 0 51.428571 0 2252.180952 Tm 25.297222 0 Td 44.444444 Tz (plasty, ) Tj 2.022222 0 Td 45.37037 Tz (in ) Tj 0.719444 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 51.851852 Tz (case ) Tj 1.380556 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 46.720679 Tz (diarthrodial ) Tj 3.519444 0 Td 43.518519 Tz (joints, ) Tj 1.963889 0 Td 59.41358 Tz (and ) Tj 1.244444 0 Td 50.434028 Tz (Surgical-induced ) Tj 51.428571 0 0 51.428571 0 2207.339713 Tm 25.297222 0 Td 50.411523 Tz (ankylosis ) Tj 2.877778 0 Td 48.611111 Tz (for ) Tj 1.011111 0 Td 46.8107 Tz (vertebral ) Tj 2.722222 0 Td 49.151235 Tz (fusion ) Tj 1.925 0 Td 50.771605 Tz (are ) Tj 1.030556 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 58.333333 Tz (most ) Tj 1.536111 0 Td 70.216049 Tz (common ) Tj 2.683333 0 Td 54.282407 Tz (interven ) Tj 51.428571 0 0 51.428571 0 2170.43 Tm 25.277778 0 Td 47.314815 Tz (tions ) Tj 1.691667 0 Td 59.953704 Tz (employed ) Tj 3.15 0 Td 47.530864 Tz (for ) Tj 1.069444 0 Td 50.555556 Tz (these ) Tj 1.788889 0 Td 52.599715 Tz (unsalvageable ) Tj 4.297222 0 Td 43.518519 Tz (joints. ) Tj 2.1 0 Td 48.611111 Tz (Spine. ) Tj 2.197222 0 Td 66.975309 Tz (Low ) Tj 47.142857 0 0 47.142857 0 2126 Tm 27.59697 0 Td 65.40404 Tz (Back ) Tj 1.69697 0 Td 68.350168 Tz (and ) Tj 1.336364 0 Td 66.287879 Tz (Neck ) Tj 1.760606 0 Td 50.909091 Tz (Pain, ) Tj 1.739394 0 Td 47.727273 Tz (in ) Tj 0.763636 0 Td 60.10101 Tz (United ) Tj 2.227273 0 Td 53.030303 Tz (States ) Tj 2.078788 0 Td 67.171717 Tz (Bone ) Tj 1.760606 0 Td 65.993266 Tz (and ) Tj 1.272727 0 Td 53.030303 Tz (Joint ) Tj 1.781818 0 Td 59.59596 Tz (Decade. ) Tj 51.428571 0 0 51.428571 0 2082 Tm 25.336111 0 Td 58.333333 Tz (The ) Tj 1.244444 0 Td 56.712963 Tz (Burden ) Tj 2.177778 0 Td 50.231481 Tz (of ) Tj 0.738889 0 Td 51.851852 Tz (Musculoskeletal ) Tj 4.880556 0 Td 52.256944 Tz (Diseases ) Tj 2.663889 0 Td 43.75 Tz (in ) Tj 0.7 0 Td 48.611111 Tz (the ) Tj 1.069444 0 Td 53.472222 Tz (United ) Tj 2.002778 0 Td 44.907407 Tz (States. ) Tj 51.428571 0 0 51.428571 0 2044.600484 Tm 25.297222 0 Td 54.444444 Tz (2008, ) Tj 1.847222 0 Td 58.738426 Tz (American ) Tj 3.072222 0 Td 66.203704 Tz (Academy ) Tj 3.013889 0 Td 59.953704 Tz (of ) Tj 0.855556 0 Td 54.208754 Tz (Orthopaedic ) Tj 3.830556 0 Td 53.292181 Tz (Surgeons: ) Tj 3.188889 0 Td 68.865741 Tz (Rose ) Tj 48.571429 0 0 48.571429 0 2001 Tm 26.826471 0 Td 57.647059 Tz (mont, ) Tj 1.955882 0 Td 34.313725 Tz (Ill. ) Tj 51.428571 0 0 51.428571 0 1960.004878 Tm 26.016667 0 Td 47.314815 Tz (Joint ) Tj 1.516667 0 Td 50.555556 Tz (prostheses ) Tj 3.130556 0 Td 57.523148 Tz (have ) Tj 1.497222 0 Td 55.092593 Tz (an ) Tj 0.816667 0 Td 53.703704 Tz (obvious ) Tj 2.352778 0 Td 54.73251 Tz (advantage ) Tj 3.091667 0 Td 53.148148 Tz (overfusion ) Tj 3.286111 0 Td 58.333333 Tz (due ) Tj 51.428571 0 0 51.428571 0 1919.478469 Tm 25.277778 0 Td 45.37037 Tz (to ) Tj 0.719444 0 Td 45.37037 Tz (at ) Tj 0.680556 0 Td 44.722222 Tz (least ) Tj 1.438889 0 Td 55.092593 Tz (a ) Tj 0.466667 0 Td 45.010288 Tz (partially ) Tj 2.586111 0 Td 52.211934 Tz (preserved ) Tj 2.955556 0 Td 45.833333 Tz (functionality, ) Tj 4.005556 0 Td 51.851852 Tz (but ) Tj 1.030556 0 Td 54.282407 Tz (both ) Tj 1.438889 0 Td 44.965278 Tz (clinical ) Tj 51.428571 0 0 51.428571 0 1875.194479 Tm 25.277778 0 Td 50.231481 Tz (treatments ) Tj 3.305556 0 Td 50.771605 Tz (are ) Tj 1.186111 0 Td 55.092593 Tz (plagued ) Tj 2.586111 0 Td 53.472222 Tz (with ) Tj 1.555556 0 Td 49.583333 Tz (exorbitant ) Tj 3.266667 0 Td 44.290123 Tz (costs, ) Tj 1.925 0 Td 48.070988 Tz (native ) Tj 2.002778 0 Td 46.450617 Tz (tissue ) Tj 51.428571 0 0 51.428571 0 1836.767962 Tm 25.297222 0 Td 49.907407 Tz (morbidity, ) Tj 3.091667 0 Td 59.41358 Tz (and ) Tj 1.186111 0 Td 50.411523 Tz (extensive ) Tj 2.858333 0 Td 49.259259 Tz (rehab/recovery, ) Tj 4.530556 0 Td 60.493827 Tz (and ) Tj 1.186111 0 Td 51.041667 Tz (they ) Tj 1.4 0 Td 65.895062 Tz (may ) Tj 1.322222 0 Td 51.041667 Tz (lead ) Tj 1.302778 0 Td 46.990741 Tz (to ) Tj 51.428571 0 0 51.428571 0 1794.237624 Tm 25.277778 0 Td 53.148148 Tz (unforeseen ) Tj 3.558333 0 Td 52.350427 Tz (complications ) Tj 4.433333 0 Td 60.493827 Tz (and ) Tj 1.380556 0 Td 51.131687 Tz (traumatic ) Tj 3.130556 0 Td 48.206019 Tz (revision ) Tj 2.683333 0 Td 47.395833 Tz (Surgery. ) Tj 51.428571 0 0 51.428571 0 1751.478469 Tm 25.297222 0 Td 53.472222 Tz (This ) Tj 1.458333 0 Td 60.185185 Tz (outcome ) Tj 2.663889 0 Td 50.925926 Tz (present ) Tj 2.313889 0 Td 54.444444 Tz (needs ) Tj 1.827778 0 Td 48.611111 Tz (for ) Tj 1.030556 0 Td 53.652263 Tz (improved, ) Tj 3.111111 0 Td 55.740741 Tz (economical ) Tj 3.5 0 Td 51.851852 Tz (treat ) Tj 51.428571 0 0 51.428571 0 1710.455346 Tm 25.297222 0 Td 60.763889 Tz (ment ) Tj 1.672222 0 Td 48.021886 Tz (modalities. ) Tj 3.363889 0 Td 48.611111 Tz (especially ) Tj 3.130556 0 Td 46.990741 Tz (in ) Tj 0.797222 0 Td 50.771605 Tz (regard ) Tj 2.022222 0 Td 45.37037 Tz (to ) Tj 0.777778 0 Td 48.611111 Tz (the ) Tj 1.088889 0 Td 44.074074 Tz (disc. ) Tj 1.575 0 Td 63.194444 Tz (Khan ) Tj 1.75 0 Td 70.216049 Tz (SN. ) Tj 51.428571 0 0 51.428571 0 1670.790588 Tm 25.297222 0 Td 58.333333 Tz (and ) Tj 1.186111 0 Td 45.37037 Tz (Stirling ) Tj 2.275 0 Td 44.560185 Tz (A.J. ) Tj 1.205556 0 Td 50.605413 Tz (Controversial ) Tj 4.083333 0 Td 47.530864 Tz (topics ) Tj 1.866667 0 Td 46.990741 Tz (in ) Tj 0.7 0 Td 48.206019 Tz (Surgery: ) Tj 2.469444 0 Td 51.311728 Tz (degenerative ) Tj 52.857143 0 0 52.857143 0 1627.365385 Tm 24.613514 0 Td 47.297297 Tz (disc ) Tj 1.267568 0 Td 45.326577 Tz (disease: ) Tj 2.345946 0 Td 47.297297 Tz (disc ) Tj 1.286486 0 Td 49.662162 Tz (replacement. ) Tj 3.689189 0 Td 48.873874 Tz (Against. ) Tj 2.478378 0 Td 63.063063 Tz (Ann ) Tj 1.324324 0 Td 63.063063 Tz (R ) Tj 0.510811 0 Td 49.662162 Tz (Coll ) Tj 1.305405 0 Td 55.968468 Tz (Surg ) Tj 1.494595 0 Td 55.18018 Tz (Engl ) Tj 51.428571 0 0 51.428571 0 1582.282828 Tm 25.297222 0 Td 55.092593 Tz (2007; ) Tj 1.905556 0 Td 48.611111 Tz (89\(1\): ) Tj 2.002778 0 Td 45.37037 Tz (p. ) Tj 0.816667 0 Td 49.907407 Tz (6-11. ) Tj 1.75 0 Td 59.548611 Tz (Wilson-MacDonald ) Tj 5.95 0 Td 38.888889 Tz (J, ) Tj 0.719444 0 Td 59.41358 Tz (and ) Tj 1.341667 0 Td 56.17284 Tz (Boeree ) Tj 2.275 0 Td 56.712963 Tz (N. ) Tj 51.428571 0 0 51.428571 0 1543.9601 Tm 25.297222 0 Td 50.356125 Tz (Controversial ) Tj 4.219444 0 Td 48.070988 Tz (topics ) Tj 2.022222 0 Td 45.37037 Tz (in ) Tj 0.875 0 Td 48.206019 Tz (Surgery: ) Tj 2.644444 0 Td 51.041667 Tz (degenerative ) Tj 3.966667 0 Td 47.800926 Tz (disc ) Tj 1.438889 0 Td 46.990741 Tz (disease: ) Tj 51.428571 0 0 51.428571 0 1502.604061 Tm 25.297222 0 Td 48.611111 Tz (disc ) Tj 1.380556 0 Td 51.311728 Tz (replacement. ) Tj 3.927778 0 Td 45.37037 Tz (For. ) Tj 1.283333 0 Td 64.814815 Tz (Ann ) Tj 1.4 0 Td 71.296296 Tz (R ) Tj 0.641667 0 Td 50.231481 Tz (Coll ) Tj 1.4 0 Td 55.902778 Tz (Surg ) Tj 1.575 0 Td 55.092593 Tz (Engl ) Tj 1.497222 0 Td 54.444444 Tz (2007; ) Tj 1.847222 0 Td 48.070988 Tz (89\(1\): ) Tj 1.944444 0 Td 45.37037 Tz (p. ) Tj 51.428571 0 0 51.428571 0 1459.144431 Tm 25.316667 0 Td 49.259259 Tz (6-11. ) Tj 1.652778 0 Td 56.712963 Tz (Although ) Tj 2.897222 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 51.203704 Tz (pains ) Tj 1.711111 0 Td 59.41358 Tz (and ) Tj 1.244444 0 Td 47.962963 Tz (costs ) Tj 1.613889 0 Td 50.231481 Tz (associated ) Tj 3.188889 0 Td 52.662037 Tz (with ) Tj 1.458333 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 51.851852 Tz (treat ) Tj 51.428571 0 0 51.428571 0 1416.482587 Tm 25.297222 0 Td 60.763889 Tz (ment ) Tj 1.672222 0 Td 55.092593 Tz (of ) Tj 0.797222 0 Td 46.720679 Tz (diarthrodial ) Tj 3.5 0 Td 46.450617 Tz (joints ) Tj 1.866667 0 Td 55.092593 Tz (such ) Tj 1.516667 0 Td 50.231481 Tz (as ) Tj 0.797222 0 Td 51.041667 Tz (hips ) Tj 1.419444 0 Td 58.333333 Tz (and ) Tj 1.225 0 Td 55.740741 Tz (knees ) Tj 1.866667 0 Td 38.888889 Tz (is ) Tj 0.680556 0 Td 50.925926 Tz (evident ) Tj 51.428571 0 0 51.428571 0 1379.385943 Tm 25.277778 0 Td 54.166667 Tz (through ) Tj 2.469444 0 Td 49.691358 Tz (the ) Tj 1.088889 0 Td 53.472222 Tz (long ) Tj 1.497222 0 Td 53.472222 Tz (road ) Tj 1.458333 0 Td 56.712963 Tz (of ) Tj 0.777778 0 Td 52.662037 Tz (pain ) Tj 1.497222 0 Td 61.25 Tz (management ) Tj 3.888889 0 Td 50.462963 Tz (leading ) Tj 2.313889 0 Td 45.37037 Tz (to ) Tj 0.738889 0 Td 58.333333 Tz (artho ) Tj 51.428571 0 0 51.428571 0 1334.790244 Tm 25.297222 0 Td 43.981481 Tz (plasty. ) Tj 2.061111 0 Td 56.17284 Tz (Hunter ) Tj 2.158333 0 Td 62.654321 Tz (DJ, ) Tj 1.263889 0 Td 60.493827 Tz (and ) Tj 1.244444 0 Td 66.435185 Tz (Lo ) Tj 0.952778 0 Td 45.37037 Tz (G. ) Tj 0.719444 0 Td 53.472222 Tz (H. ) Tj 0.816667 0 Td 62.654321 Tz (The ) Tj 1.302778 0 Td 61.898148 Tz (management ) Tj 3.830556 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 55.555556 Tz (osteoar ) Tj 51.428571 0 0 51.428571 0 1292.628571 Tm 25.277778 0 Td 39.293981 Tz (thritis: ) Tj 2.041667 0 Td 56.712963 Tz (an ) Tj 0.836111 0 Td 54.6875 Tz (overview ) Tj 2.780556 0 Td 59.41358 Tz (and ) Tj 1.225 0 Td 42.12963 Tz (call ) Tj 1.147222 0 Td 46.990741 Tz (to ) Tj 0.7 0 Td 50.378788 Tz (appropriate ) Tj 3.480556 0 Td 50.231481 Tz (conservative ) Tj 3.713889 0 Td 51.851852 Tz (treat ) Tj 51.428571 0 0 51.428571 0 1250.645933 Tm 25.297222 0 Td 53.796296 Tz (ment. ) Tj 1.827778 0 Td 65.462963 Tz (Rheum ) Tj 2.197222 0 Td 51.851852 Tz (Dis ) Tj 1.108333 0 Td 64.814815 Tz (ClinNAm2008:34: ) Tj 6.008333 0 Td 42.12963 Tz (p. ) Tj 0.7 0 Td 53.472222 Tz (689-712. ) Tj 2.761111 0 Td 52.572016 Tz (Surgeons, ) Tj 51.428571 0 0 51.428571 0 1211.369434 Tm 25.316667 0 Td 42.718855 Tz (Scientists, ) Tj 2.975 0 Td 59.41358 Tz (and ) Tj 1.186111 0 Td 52.211934 Tz (engineers ) Tj 2.975 0 Td 55.902778 Tz (have ) Tj 1.516667 0 Td 64.814815 Tz (made ) Tj 1.730556 0 Td 64.814815 Tz (many ) Tj 1.75 0 Td 50.636574 Tz (attempts ) Tj 2.605556 0 Td 46.990741 Tz (to ) Tj 0.719444 0 Td 47.685185 Tz (restore ) Tj 51.428571 0 0 51.428571 0 1169.774153 Tm 25.297222 0 Td 47.43266 Tz (functional, ) Tj 3.519444 0 Td 53.364198 Tz (biomechanically ) Tj 5.230556 0 Td 46.990741 Tz (stable ) Tj 2.080556 0 Td 46.450617 Tz (cartilage ) Tj 2.897222 0 Td 50.771605 Tz (within ) Tj 2.216667 0 Td 51.203704 Tz (these ) Tj 51.428571 0 0 51.428571 0 1130.431373 Tm 25.297222 0 Td 46.180556 Tz (articulating ) Tj 3.5 0 Td 45.910494 Tz (joints ) Tj 1.886111 0 Td 53.240741 Tz (through ) Tj 2.469444 0 Td 48.611111 Tz (grafting ) Tj 2.527778 0 Td 53.472222 Tz (procedures ) Tj 3.441667 0 Td 52.662037 Tz (with ) Tj 1.536111 0 Td 36.188272 Tz (little ) Tj 1.536111 0 Td 45.37037 Tz (to ) Tj 51.428571 0 0 51.428571 0 1084.62963 Tm 25.297222 0 Td 59.953704 Tz (no ) Tj 1.05 0 Td 49.907407 Tz (repeatable ) Tj 3.286111 0 Td 48.206019 Tz (success. ) Tj 2.683333 0 Td 60.277778 Tz (Revel ) Tj 2.1 0 Td 84.259259 Tz (C ) Tj 0.797222 0 Td 69.675926 Tz (M, ) Tj 1.108333 0 Td 60.493827 Tz (and ) Tj 1.361111 0 Td 54.444444 Tz (Athanasiou ) Tj 3.616667 0 Td 84.259259 Tz (K ) Tj 0.777778 0 Td 55.092593 Tz (A. ) Tj 51.428571 0 0 51.428571 0 1045.738975 Tm 25.316667 0 Td 54.166667 Tz (Success ) Tj 2.45 0 Td 44.722222 Tz (rates ) Tj 1.516667 0 Td 58.333333 Tz (and ) Tj 1.205556 0 Td 58.627946 Tz (immunologic ) Tj 4.044444 0 Td 51.851852 Tz (responses ) Tj 2.994444 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 49.907407 Tz (autogenic, ) Tj 3.169444 0 Td 55.092593 Tz (allo ) Tj 51.428571 0 0 51.428571 0 1004.455206 Tm 25.297222 0 Td 48.611111 Tz (genic, ) Tj 1.963889 0 Td 58.333333 Tz (and ) Tj 1.263889 0 Td 54.372428 Tz (Xenogenic ) Tj 3.188889 0 Td 50.231481 Tz (treatments ) Tj 3.208333 0 Td 48.611111 Tz (to ) Tj 0.797222 0 Td 48.070988 Tz (repair ) Tj 1.886111 0 Td 45.730453 Tz (articular ) Tj 2.644444 0 Td 46.8107 Tz (cartilage ) Tj 51.428571 0 0 51.428571 0 959.008902 Tm 25.297222 0 Td 46.585648 Tz (defects. ) Tj 2.488889 0 Td 49.151235 Tz (Tissue ) Tj 2.002778 0 Td 62.654321 Tz (Eng ) Tj 1.302778 0 Td 51.041667 Tz (Part ) Tj 1.419444 0 Td 71.296296 Tz (B ) Tj 0.641667 0 Td 58.333333 Tz (Rev ) Tj 1.205556 0 Td 55.740741 Tz (2009; ) Tj 1.944444 0 Td 45.910494 Tz (15\(1\): ) Tj 1.886111 0 Td 43.75 Tz (p. ) Tj 0.797222 0 Td 47.314815 Tz (1-15. ) Tj 51.428571 0 0 51.428571 0 920.082927 Tm 26.016667 0 Td 63.734568 Tz (Use ) Tj 1.263889 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 48.611111 Tz (resorbable, ) Tj 3.383333 0 Td 49.151235 Tz (regenerative ) Tj 3.675 0 Td 48.251029 Tz (synthetic ) Tj 2.741667 0 Td 49.789562 Tz (biomaterial ) Tj 3.441667 0 Td 83.179012 Tz (com ) Tj 51.428571 0 0 51.428571 0 878.343864 Tm 25.297222 0 Td 47.685185 Tz (posites ) Tj 2.197222 0 Td 51.311728 Tz (within ) Tj 1.944444 0 Td 46.450617 Tz (joints ) Tj 1.827778 0 Td 40.509259 Tz (is ) Tj 0.661111 0 Td 55.092593 Tz (an ) Tj 0.836111 0 Td 44.722222 Tz (attractive ) Tj 2.838889 0 Td 45.664983 Tz (possibility ) Tj 3.188889 0 Td 45.37037 Tz (but, ) Tj 1.263889 0 Td 52.932099 Tz (has ) Tj 1.108333 0 Td 53.148148 Tz (tradi ) Tj 51.428571 0 0 51.428571 0 838.359318 Tm 25.277778 0 Td 46.990741 Tz (tionally ) Tj 2.469444 0 Td 56.17284 Tz (performed ) Tj 3.247222 0 Td 44.560185 Tz (less ) Tj 1.283333 0 Td 46.548822 Tz (effectively ) Tj 3.305556 0 Td 51.851852 Tz (than ) Tj 1.458333 0 Td 48.611111 Tz (grafting ) Tj 2.547222 0 Td 59.41358 Tz (and ) Tj 1.283333 0 Td 44.907407 Tz (results ) Tj 51.428571 0 0 51.428571 0 794.352941 Tm 25.277778 0 Td 57.523148 Tz (have ) Tj 1.594444 0 Td 56.712963 Tz (been ) Tj 1.594444 0 Td 51.311728 Tz (highly ) Tj 2.119444 0 Td 45.010288 Tz (variable. ) Tj 2.683333 0 Td 56.712963 Tz (Although ) Tj 2.955556 0 Td 40.277778 Tz (initial ) Tj 1.944444 0 Td 51.388889 Tz (Success ) Tj 2.411111 0 Td 37.268519 Tz (is ) Tj 0.680556 0 Td 83.179012 Tz (com ) Tj 51.428571 0 0 51.428571 0 753.515605 Tm 25.297222 0 Td 60.763889 Tz (mon, ) Tj 1.788889 0 Td 52.350427 Tz (osteochondral ) Tj 4.394444 0 Td 49.016204 Tz (grafting ) Tj 2.644444 0 Td 48.287037 Tz (\(allogenic ) Tj 3.208333 0 Td 46.990741 Tz (tissue ) Tj 2.002778 0 Td 44.290123 Tz (graft\) ) Tj 1.925 0 Td 83.179012 Tz (com ) Tj 51.428571 0 0 51.428571 0 712.560803 Tm 25.297222 0 Td 61.574074 Tz (monly ) Tj 2.002778 0 Td 40.833333 Tz (fails ) Tj 1.380556 0 Td 50.231481 Tz (long-term. ) Tj 3.15 0 Td 58.333333 Tz (These ) Tj 1.905556 0 Td 48.070988 Tz (inconsistent ) Tj 3.577778 0 Td 59.41358 Tz (and ) Tj 1.225 0 Td 45.37037 Tz (failed ) Tj 1.75 0 Td 51.446759 Tz (attempts ) Tj 51.428571 0 0 51.428571 0 672.819071 Tm 25.316667 0 Td 51.851852 Tz (Suggest ) Tj 2.391667 0 Td 50.771605 Tz (the ) Tj 1.127778 0 Td 52.5 Tz (underlying ) Tj 3.383333 0 Td 51.851852 Tz (causes ) Tj 2.138889 0 Td 55.092593 Tz (of ) Tj 0.816667 0 Td 46.450617 Tz (cartilage ) Tj 2.722222 0 Td 51.851852 Tz (degeneration ) Tj 3.986111 0 Td 49.691358 Tz (are ) Tj 51.428571 0 0 51.428571 0 628.727517 Tm 25.297222 0 Td 51.851852 Tz (not ) Tj 1.361111 0 Td 53.796296 Tz (being ) Tj 2.061111 0 Td 54.552469 Tz (circumvented ) Tj 4.316667 0 Td 61.574074 Tz (by ) Tj 1.186111 0 Td 49.907407 Tz (these ) Tj 1.925 0 Td 43.75 Tz (clinical ) Tj 2.547222 0 Td 47.890947 Tz (practices ) Tj 2.994444 0 Td 60.493827 Tz (and ) Tj 51.428571 0 0 51.428571 0 588.86578 Tm 25.297222 0 Td 51.851852 Tz (researched ) Tj 3.480556 0 Td 48.316498 Tz (treatments. ) Tj 3.538889 0 Td 61.844136 Tz (Accompanying ) Tj 4.744444 0 Td 47.314815 Tz (joint ) Tj 1.769444 0 Td 50.106838 Tz (degeneration, ) Tj 51.428571 0 0 51.428571 0 547.543391 Tm 25.297222 0 Td 58.333333 Tz (and ) Tj 1.438889 0 Td 52.146465 Tz (manifesting ) Tj 3.772222 0 Td 48.611111 Tz (prior ) Tj 1.769444 0 Td 48.611111 Tz (to ) Tj 0.972222 0 Td 55.497685 Tz (complete ) Tj 3.013889 0 Td 46.8107 Tz (cartilage ) Tj 2.877778 0 Td 42.939815 Tz (failure, ) Tj 2.469444 0 Td 37.268519 Tz (is ) Tj 0.836111 0 Td 55.092593 Tz (a ) Tj 51.428571 0 0 51.428571 0 503.630807 Tm 25.297222 0 Td 57.253086 Tz (change ) Tj 2.275 0 Td 45.37037 Tz (in ) Tj 0.758333 0 Td 51.851852 Tz (the ) Tj 1.127778 0 Td 52.824074 Tz (underlying ) Tj 3.383333 0 Td 53.619529 Tz (Subchondral ) Tj 3.772222 0 Td 58.333333 Tz (and ) Tj 1.263889 0 Td 50.555556 Tz (cancellous ) Tj 3.286111 0 Td 52.5 Tz (bone. ) Tj 51.428571 0 0 51.428571 0 463.391608 Tm 25.297222 0 Td 62.384259 Tz (Most ) Tj 1.652778 0 Td 50.925926 Tz (notably ) Tj 2.255556 0 Td 40.509259 Tz (is ) Tj 0.622222 0 Td 55.452675 Tz (advancing ) Tj 3.111111 0 Td 45.37037 Tz (calcified ) Tj 2.547222 0 Td 46.8107 Tz (cartilage ) Tj 2.625 0 Td 51.527778 Tz (\(tidemark\) ) Tj 3.169444 0 Td 49.907407 Tz (early ) Tj 51.428571 0 0 51.428571 0 420.945223 Tm 25.297222 0 Td 45.37037 Tz (in ) Tj 0.875 0 Td 49.691358 Tz (the ) Tj 1.186111 0 Td 46.990741 Tz (disease, ) Tj 2.566667 0 Td 60.493827 Tz (and ) Tj 1.380556 0 Td 52.5 Tz (osteophyte ) Tj 3.480556 0 Td 52.572016 Tz (formation ) Tj 3.15 0 Td 56.17284 Tz (around ) Tj 2.352778 0 Td 51.446759 Tz (invading ) Tj 51.428571 0 0 51.428571 0 377.359801 Tm 25.297222 0 Td 48.611111 Tz (vessels ) Tj 2.236111 0 Td 42.12963 Tz (in ) Tj 0.7 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 56.712963 Tz (advanced ) Tj 2.877778 0 Td 41.589506 Tz (state. ) Tj 1.652778 0 Td 55.497685 Tz (Goldring ) Tj 2.819444 0 Td 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) Tj 1.438889 0 Td 53.472222 Tz (This ) Tj 1.419444 0 Td 54.503367 Tz (composition ) Tj 3.733333 0 Td 37.268519 Tz (is ) Tj 0.622222 0 Td 64.00463 Tz (made ) Tj 1.652778 0 Td 53.472222 Tz (of ) Tj 0.719444 0 Td 52.175926 Tz (analginate ) Tj 3.208333 0 Td 54.282407 Tz (hydrogel ) Tj 2.722222 0 Td 55.092593 Tz (having ) Tj 51.428571 0 0 51.428571 0 1752.847255 Tm 6.358333 0 Td 62.384259 Tz (embedded ) Tj 3.188889 0 Td 47.685185 Tz (therein ) Tj 2.236111 0 Td 56.17284 Tz (nonporous ) Tj 3.363889 0 Td 48.070988 Tz (B-tricalcium ) Tj 3.675 0 Td 54.73251 Tz (phosphate ) Tj 3.15 0 Td 61.574074 Tz (aggre ) Tj 51.428571 0 0 51.428571 0 1709.73317 Tm 6.358333 0 Td 49.259259 Tz (gates ) Tj 1.652778 0 Td 54.552469 Tz (having ) Tj 2.1 0 Td 55.092593 Tz (a ) Tj 0.505556 0 Td 53.472222 Tz (diameter ) Tj 2.683333 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 57.407407 Tz (between ) Tj 2.644444 0 Td 56.018519 Tz (125-150 ) Tj 2.547222 0 Td 72.916667 Tz (um ) Tj 1.05 0 Td 58.333333 Tz (and ) Tj 1.186111 0 Td 55.092593 Tz (a ) Tj 0.486111 0 Td 60.925926 Tz (bioac ) Tj 51.428571 0 0 51.428571 0 1668.960479 Tm 6.338889 0 Td 45.37037 Tz (tive ) Tj 1.205556 0 Td 48.611111 Tz (agent. ) Tj 1.944444 0 Td 46.990741 Tz (In ) Tj 0.719444 0 Td 49.691358 Tz (the ) Tj 1.011111 0 Td 49.691358 Tz (preferred ) Tj 2.819444 0 Td 60.925926 Tz (embodiment ) Tj 3.772222 0 Td 49.691358 Tz (the ) Tj 1.011111 0 Td 49.331276 Tz (bioactive ) Tj 2.8 0 Td 52.5 Tz (agent ) Tj 1.730556 0 Td 38.888889 Tz (is ) Tj 52.857143 0 0 52.857143 0 1625.110115 Tm 6.186486 0 Td 50 Tz (derived ) Tj 2.327027 0 Td 55.968468 Tz (from ) Tj 1.589189 0 Td 43.443443 Tz (platelets ) Tj 2.572973 0 Td 56.756757 Tz (and ) Tj 1.248649 0 Td 48.348348 Tz (the ) Tj 1.059459 0 Td 47.997998 Tz (bioactive ) Tj 2.8 0 Td 51.711712 Tz (agent ) Tj 1.759459 0 Td 36.261261 Tz (is ) Tj 0.662162 0 Td 45.720721 Tz (at ) Tj 0.775676 0 Td 42.252252 Tz (least ) Tj 1.513514 0 Td 58.333333 Tz (50 ) Tj 51.428571 0 0 51.428571 0 1587.722488 Tm 6.358333 0 Td 51.388889 Tz (percent ) Tj 2.294444 0 Td 59.953704 Tz (by ) Tj 0.933333 0 Td 59.41358 Tz (volume ) Tj 2.275 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 50.771605 Tz (hydrogel. ) Tj 2.897222 0 Td 48.611111 Tz (Additionally, ) Tj 3.966667 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 73.888889 Tz (compo ) Tj 51.428571 0 0 51.428571 0 1541.678887 Tm 6.377778 0 Td 44.290123 Tz (sition ) Tj 1.905556 0 Td 54.012346 Tz (can ) Tj 1.283333 0 Td 48.611111 Tz (also ) Tj 1.458333 0 Td 51.388889 Tz (include ) Tj 2.469444 0 Td 53.703704 Tz (calcium ) Tj 2.566667 0 Td 47.138047 Tz (distributed ) Tj 3.363889 0 Td 53.796296 Tz (throughout ) Tj 3.519444 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 1502.517028 Tm 6.358333 0 Td 52.932099 Tz (matrix ) Tj 2.080556 0 Td 59.953704 Tz (of ) Tj 0.797222 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 51.131687 Tz (hydrogel. ) Tj 51.428571 0 0 51.428571 0 1459.394015 Tm 7.116667 0 Td 46.990741 Tz (In ) Tj 0.680556 0 Td 52.777778 Tz (another ) Tj 2.313889 0 Td 57.744108 Tz (embodiment, ) Tj 3.927778 0 Td 55.092593 Tz (a ) Tj 0.447222 0 Td 43.209877 Tz (kit ) Tj 0.875 0 Td 40.509259 Tz (is ) Tj 0.583333 0 Td 54.282407 Tz (provided ) Tj 2.741667 0 Td 53.472222 Tz (with ) Tj 1.380556 0 Td 55.092593 Tz (a ) Tj 0.466667 0 Td 37.592593 Tz (first ) Tj 1.244444 0 Td 57.253086 Tz (mixing ) Tj 51.428571 0 0 51.428571 0 1417.742574 Tm 6.358333 0 Td 50.771605 Tz (container ) Tj 2.994444 0 Td 50.87963 Tz (containing ) Tj 3.286111 0 Td 58.333333 Tz (an ) Tj 0.933333 0 Td 48.611111 Tz (alginate ) Tj 2.605556 0 Td 46.450617 Tz (solution, ) Tj 2.761111 0 Td 52.73569 Tz (connectable ) Tj 3.733333 0 Td 46.990741 Tz (to ) Tj 0.836111 0 Td 55.092593 Tz (a ) Tj 51.428571 0 0 51.428571 0 1376.221053 Tm 6.377778 0 Td 55.632716 Tz (second ) Tj 2.488889 0 Td 56.17284 Tz (mixing ) Tj 2.45 0 Td 46.666667 Tz (container. ) Tj 3.266667 0 Td 63.734568 Tz (The ) Tj 1.594444 0 Td 55.632716 Tz (second ) Tj 2.45 0 Td 57.79321 Tz (mixing ) Tj 2.508333 0 Td 50.411523 Tz (container ) Tj 48.571429 0 0 48.571429 0 1334 Tm 6.732353 0 Td 53.186275 Tz (includes ) Tj 2.820588 0 Td 46.323529 Tz (at ) Tj 0.823529 0 Td 46.666667 Tz (least ) Tj 1.626471 0 Td 62.908497 Tz (one ) Tj 1.358824 0 Td 57.843137 Tz (calcium ) Tj 2.676471 0 Td 41.862745 Tz (salt, ) Tj 1.523529 0 Td 48.039216 Tz (in ) Tj 0.823529 0 Td 61.764706 Tz (a ) Tj 0.617647 0 Td 47.352941 Tz (sufficient ) Tj 3.067647 0 Td 64.052288 Tz (amount ) Tj 2.552941 0 Td 49.754902 Tz (to ) Tj 51.428571 0 0 51.428571 0 1294.604167 Tm 6.358333 0 Td 42.12963 Tz (initiate ) Tj 2.333333 0 Td 52.546296 Tz (polymerization ) Tj 4.744444 0 Td 58.333333 Tz (of ) Tj 0.933333 0 Td 49.691358 Tz (the ) Tj 1.205556 0 Td 48.206019 Tz (alginate ) Tj 2.644444 0 Td 48.206019 Tz (solution ) Tj 2.663889 0 Td 45.37037 Tz (to ) Tj 0.875 0 Td 58.333333 Tz (form ) Tj 1.75 0 Td 55.092593 Tz (a ) Tj 51.428571 0 0 51.428571 0 1251.891566 Tm 6.338889 0 Td 51.49177 Tz (hydrogel, ) Tj 2.955556 0 Td 54.62963 Tz (wherein ) Tj 2.45 0 Td 50.771605 Tz (the ) Tj 1.05 0 Td 54.166667 Tz (calcium ) Tj 2.45 0 Td 42.12963 Tz (salt ) Tj 1.166667 0 Td 37.268519 Tz (is ) Tj 0.622222 0 Td 56.532922 Tz (nonporous ) Tj 3.422222 0 Td 46.720679 Tz (B-tricalcium ) Tj 51.428571 0 0 51.428571 0 1209.564792 Tm 6.358333 0 Td 51.851852 Tz (phosphate. ) Tj 3.363889 0 Td 45.37037 Tz (In ) Tj 0.758333 0 Td 42.939815 Tz (this ) Tj 1.205556 0 Td 58.038721 Tz (embodiment, ) Tj 4.025 0 Td 46.990741 Tz (either ) Tj 1.847222 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 38.240741 Tz (first ) Tj 1.283333 0 Td 55.092593 Tz (or ) Tj 0.816667 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 57.253086 Tz (second ) Tj 51.428571 0 0 51.428571 0 1166.87218 Tm 6.358333 0 Td 50.771605 Tz (container ) Tj 2.955556 0 Td 37.268519 Tz (is ) Tj 0.680556 0 Td 54.73251 Tz (connected ) Tj 3.15 0 Td 48.611111 Tz (to ) Tj 0.797222 0 Td 58.333333 Tz (a ) Tj 0.602778 0 Td 51.851852 Tz (needle ) Tj 2.1 0 Td 56.712963 Tz (of ) Tj 0.836111 0 Td 54.444444 Tz (dimensions ) Tj 3.519444 0 Td 51.851852 Tz (not ) Tj 1.166667 0 Td 46.990741 Tz (larger ) Tj 51.428571 0 0 51.428571 0 1125.7866 Tm 6.338889 0 Td 52.662037 Tz (than ) Tj 1.438889 0 Td 46.990741 Tz (that ) Tj 1.302778 0 Td 56.712963 Tz (of ) Tj 0.777778 0 Td 55.092593 Tz (a ) Tj 0.563889 0 Td 55.092593 Tz (16 ) Tj 0.836111 0 Td 58.333333 Tz (gauge ) Tj 1.963889 0 Td 48.148148 Tz (needle. ) Tj 51.428571 0 0 51.428571 0 1086.316285 Tm 7.097222 0 Td 62.654321 Tz (The ) Tj 1.380556 0 Td 56.712963 Tz (examples ) Tj 2.994444 0 Td 61.574074 Tz (show ) Tj 1.788889 0 Td 49.691358 Tz (the ) Tj 1.147222 0 Td 52.932099 Tz (use ) Tj 1.225 0 Td 58.333333 Tz (of ) Tj 0.836111 0 Td 50.555556 Tz (these ) Tj 1.788889 0 Td 54.444444 Tz (composites ) Tj 3.5 0 Td 51.851852 Tz (as ) Tj 0.913889 0 Td 48.611111 Tz (cost ) Tj 51.428571 0 0 51.428571 0 1042.846154 Tm 6.358333 0 Td 42.453704 Tz (efficient, ) Tj 2.819444 0 Td 49.691358 Tz (robust ) Tj 2.022222 0 Td 59.953704 Tz (bone ) Tj 1.672222 0 Td 46.666667 Tz (graft ) Tj 1.633333 0 Td 44.830247 Tz (substitutes. ) Tj 3.5 0 Td 63.194444 Tz (Most ) Tj 1.769444 0 Td 51.388889 Tz (notably ) Tj 2.45 0 Td 37.268519 Tz (is ) Tj 0.719444 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 1001.605804 Tm 6.358333 0 Td 48.905724 Tz (application ) Tj 3.461111 0 Td 49.691358 Tz (for ) Tj 1.05 0 Td 49.691358 Tz (the ) Tj 1.108333 0 Td 58.333333 Tz (enhancement ) Tj 4.044444 0 Td 58.333333 Tz (of ) Tj 0.855556 0 Td 53.652263 Tz (nonunion, ) Tj 3.15 0 Td 46.8107 Tz (necrosis, ) Tj 2.741667 0 Td 60.493827 Tz (and ) Tj 51.428571 0 0 51.428571 0 964.179245 Tm 6.358333 0 Td 48.611111 Tz (cyst ) Tj 1.341667 0 Td 47.193287 Tz (osteo-therapies. ) Tj 4.686111 0 Td 48.611111 Tz (Additionally, ) Tj 3.966667 0 Td 49.907407 Tz (these ) Tj 1.672222 0 Td 54.12037 Tz (composites ) Tj 3.402778 0 Td 56.712963 Tz (have ) Tj 1.516667 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 921.683157 Tm 6.358333 0 Td 43.981481 Tz (ability ) Tj 1.963889 0 Td 48.611111 Tz (to ) Tj 0.738889 0 Td 56.018519 Tz (remodel ) Tj 2.488889 0 Td 50.605413 Tz (withstanding, ) Tj 4.083333 0 Td 49.691358 Tz (yet ) Tj 1.030556 0 Td 49.583333 Tz (unhealthy, ) Tj 3.130556 0 Td 53.240741 Tz (osseous ) Tj 2.372222 0 Td 46.450617 Tz (tissue ) Tj 51.428571 0 0 51.428571 0 877.938979 Tm 6.358333 0 Td 50.231481 Tz (as ) Tj 0.836111 0 Td 37.268519 Tz (is ) Tj 0.661111 0 Td 53.472222 Tz (seen ) Tj 1.497222 0 Td 50.636574 Tz (adjacent ) Tj 2.605556 0 Td 46.990741 Tz (to ) Tj 0.797222 0 Td 47.170782 Tz (Vertebral ) Tj 2.741667 0 Td 59.41358 Tz (and ) Tj 1.263889 0 Td 46.720679 Tz (diarthrodial ) Tj 3.558333 0 Td 45.37037 Tz (joints ) Tj 1.847222 0 Td 55.740741 Tz (prone ) Tj 51.428571 0 0 51.428571 0 837.803805 Tm 6.358333 0 Td 48.611111 Tz (for ) Tj 0.991667 0 Td 42.849794 Tz (arthritic ) Tj 2.488889 0 Td 51.58179 Tz (degeneration ) Tj 3.888889 0 Td 58.333333 Tz (and ) Tj 1.186111 0 Td 48.971193 Tz (highlight ) Tj 2.8 0 Td 49.691358 Tz (the ) Tj 1.030556 0 Td 44.373219 Tz (possibilities ) Tj 3.655556 0 Td 55.092593 Tz (of ) Tj 0.719444 0 Td 61.574074 Tz (an ) Tj 51.428571 0 0 51.428571 0 797.141509 Tm 6.358333 0 Td 47.962963 Tz (early ) Tj 1.633333 0 Td 49.421296 Tz (preventative ) Tj 3.752778 0 Td 55.092593 Tz (remodeling ) Tj 3.461111 0 Td 47.800926 Tz (therapy. ) Tj 2.547222 0 Td 52.391975 Tz (Furthermore, ) Tj 3.966667 0 Td 50.462963 Tz (closure ) Tj 51.428571 0 0 51.428571 0 752.805825 Tm 6.358333 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 57.118056 Tz (nonunion ) Tj 2.936111 0 Td 46.990741 Tz (fracture ) Tj 2.430556 0 Td 55.092593 Tz (ends ) Tj 1.497222 0 Td 58.333333 Tz (and ) Tj 1.205556 0 Td 59.953704 Tz (bone ) Tj 1.613889 0 Td 48.611111 Tz (cyst ) Tj 1.302778 0 Td 51.527778 Tz (perimeters ) Tj 3.266667 0 Td 49.691358 Tz (are ) Tj 1.069444 0 Td 56.712963 Tz (typi ) Tj 51.428571 0 0 51.428571 0 712.127175 Tm 6.358333 0 Td 46.666667 Tz (cally ) Tj 1.788889 0 Td 58.333333 Tz (of ) Tj 0.972222 0 Td 55.092593 Tz (a ) Tj 0.719444 0 Td 44.560185 Tz (cortical ) Tj 2.547222 0 Td 46.759259 Tz (nature. ) Tj 2.333333 0 Td 50.555556 Tz (Therefore, ) Tj 3.422222 0 Td 56.712963 Tz (an ) Tj 1.069444 0 Td 53.240741 Tz (implant ) Tj 2.586111 0 Td 56.712963 Tz (system ) Tj 51.428571 0 0 51.428571 0 672.379653 Tm 6.358333 0 Td 53.703704 Tz (capable ) Tj 2.488889 0 Td 55.092593 Tz (of ) Tj 0.816667 0 Td 53.06713 Tz (inducing ) Tj 2.780556 0 Td 53.472222 Tz (both ) Tj 1.555556 0 Td 48.611111 Tz (resorption ) Tj 3.188889 0 Td 56.712963 Tz (of ) Tj 0.855556 0 Td 52.211934 Tz (unhealthy ) Tj 3.072222 0 Td 59.143519 Tz (bone ) Tj 1.652778 0 Td 60.493827 Tz (and ) Tj 51.428571 0 0 51.428571 0 627.138422 Tm 6.358333 0 Td 52.932099 Tz (formation ) Tj 3.188889 0 Td 56.712963 Tz (of ) Tj 0.894444 0 Td 50.925926 Tz (healthy ) Tj 2.469444 0 Td 59.143519 Tz (bone ) Tj 1.75 0 Td 37.268519 Tz (is ) Tj 0.797222 0 Td 50.87963 Tz (warranted, ) Tj 3.344444 0 Td 60.493827 Tz (and ) Tj 1.4 0 Td 49.907407 Tz (these ) Tj 1.769444 0 Td 51.311728 Tz (events ) Tj 51.428571 0 0 51.428571 0 587.477032 Tm 6.358333 0 Td 50.636574 Tz (describe ) Tj 2.566667 0 Td 48.611111 Tz (the ) Tj 0.972222 0 Td 46.8107 Tz (potential ) Tj 2.663889 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 49.691358 Tz (the ) Tj 0.991667 0 Td 45.561002 Tz (alginate/platelet ) Tj 4.802778 0 Td 53.240741 Tz (releasate/CaPO ) Tj 45.714286 0 0 45.714286 0 549 Tm 7.175 0 Td 57.8125 Tz (system. ) Tj 48.571429 0 0 48.571429 0 503 Tm 7.535294 0 Td 49.754902 Tz (In ) Tj 0.844118 0 Td 61.764706 Tz (one ) Tj 1.338235 0 Td 61.14082 Tz (embodiment, ) Tj 4.302941 0 Td 44.607843 Tz (this ) Tj 1.338235 0 Td 52.614379 Tz (invention ) Tj 3.108824 0 Td 47.058824 Tz (relates ) Tj 2.223529 0 Td 48.039216 Tz (to ) Tj 0.823529 0 Td 58.333333 Tz (a ) Tj 0.597059 0 Td 63.480392 Tz (method ) Tj 2.532353 0 Td 48.039216 Tz (to ) Tj 51.428571 0 0 51.428571 0 462.304348 Tm 6.358333 0 Td 42.453704 Tz (facilitate ) Tj 2.702778 0 Td 59.143519 Tz (bone ) Tj 1.594444 0 Td 47.530864 Tz (repair ) Tj 1.847222 0 Td 59.953704 Tz (by ) Tj 0.875 0 Td 50.231481 Tz (administrating ) Tj 4.375 0 Td 45.37037 Tz (to ) Tj 0.738889 0 Td 51.851852 Tz (a ) Tj 0.525 0 Td 62.654321 Tz (mammalian ) Tj 3.558333 0 Td 69.135802 Tz (Sub ) Tj 51.428571 0 0 51.428571 0 419.695761 Tm 6.319444 0 Td 46.180556 Tz (ject ) Tj 1.322222 0 Td 43.75 Tz (in ) Tj 0.777778 0 Td 55.902778 Tz (need ) Tj 1.555556 0 Td 58.333333 Tz (of ) Tj 0.797222 0 Td 51.131687 Tz (treatment ) Tj 2.955556 0 Td 55.092593 Tz (an ) Tj 0.855556 0 Td 46.8107 Tz (effective ) Tj 2.741667 0 Td 60.493827 Tz (amount ) Tj 2.391667 0 Td 58.333333 Tz (of ) Tj 0.797222 0 Td 49.691358 Tz (the ) Tj 1.108333 0 Td 73.240741 Tz (compo ) Tj 51.428571 0 0 51.428571 0 377.910931 Tm 6.377778 0 Td 44.290123 Tz (sition ) Tj 2.022222 0 Td 59.953704 Tz (of ) Tj 1.069444 0 Td 42.939815 Tz (this ) Tj 1.477778 0 Td 47.638889 Tz (invention. ) Tj 3.325 0 Td 65.625 Tz (More ) Tj 2.002778 0 Td 44.123932 Tz (specifically, ) Tj 3.908333 0 Td 41.319444 Tz (this ) Tj 1.477778 0 Td 61.033951 Tz (method ) Tj 51.428571 0 0 51.428571 0 338.130024 Tm 6.358333 0 Td 50.231481 Tz (involves ) Tj 2.547222 0 Td 43.75 Tz (facilitating ) Tj 3.305556 0 Td 51.851852 Tz (osteochondral ) Tj 4.2 0 Td 59.143519 Tz (bone ) Tj 1.575 0 Td 54.768519 Tz (remodeling ) Tj 3.441667 0 Td 45.37037 Tz (in ) Tj 0.661111 0 Td 58.333333 Tz (a ) Tj 0.486111 0 Td 69.135802 Tz (Sub ) Tj 51.428571 0 0 51.428571 0 298.279799 Tm 6.319444 0 Td 46.180556 Tz (ject ) Tj 1.302778 0 Td 59.953704 Tz (by ) Tj 0.933333 0 Td 53.06713 Tz (inducing ) Tj 2.780556 0 Td 49.691358 Tz (regeneration ) Tj 3.811111 0 Td 58.333333 Tz (of ) Tj 0.797222 0 Td 43.75 Tz (this ) Tj 1.263889 0 Td 59.953704 Tz (bone ) Tj 1.613889 0 Td 46.990741 Tz (to ) Tj 0.777778 0 Td 58.333333 Tz (a ) Tj 0.525 0 Td 47.395833 Tz (healthy, ) Tj 2.527778 0 Td 64.814815 Tz (Vas ) Tj 51.428571 0 0 51.428571 0 256.231504 Tm 6.358333 0 Td 49.691358 Tz (cularized ) Tj 2.858333 0 Td 44.722222 Tz (state ) Tj 1.536111 0 Td 52.777778 Tz (capable ) Tj 2.411111 0 Td 58.333333 Tz (of ) Tj 0.797222 0 Td 51.851852 Tz (Supporting ) Tj 3.286111 0 Td 50.771605 Tz (the ) Tj 1.088889 0 Td 52.824074 Tz (underlying ) Tj 3.344444 0 Td 51.388889 Tz (hyaline ) Tj 51.428571 0 0 51.428571 0 212.832941 Tm 6.358333 0 Td 46.090535 Tz (cartilage ) Tj 2.644444 0 Td 51.851852 Tz (of ) Tj 0.7 0 Td 45.37037 Tz (articular ) Tj 2.527778 0 Td 45.37037 Tz (joints ) Tj 1.769444 0 Td 58.333333 Tz (and ) Tj 1.166667 0 Td 48.070988 Tz (spinal ) Tj 1.847222 0 Td 45.37037 Tz (discs, ) Tj 1.769444 0 Td 52.662037 Tz (both ) Tj 1.380556 0 Td 60.925926 Tz (biomechani ) Tj 51.428571 0 0 51.428571 0 172.685139 Tm 6.358333 0 Td 46.666667 Tz (cally ) Tj 1.652778 0 Td 58.333333 Tz (and ) Tj 1.302778 0 Td 48.148148 Tz (metabolically. ) Tj 4.277778 0 Td 54.282407 Tz (This ) Tj 1.555556 0 Td 51.388889 Tz (process ) Tj 2.411111 0 Td 49.826389 Tz (involves ) Tj 2.644444 0 Td 49.691358 Tz (the ) Tj 1.127778 0 Td 47.962963 Tz (steps ) Tj 1.691667 0 Td 42.12963 Tz (of: ) Tj 30 0 0 30 0 2547 Tm 41.666667 0 Td 75 Tz (10 ) Tj 30 0 0 30 0 2339 Tm 41.666667 0 Td 72.222222 Tz (15 ) Tj 30 0 0 30 0 1923 Tm 41.6 0 Td 77.777778 Tz (25 ) Tj 30 0 0 30 0 1716 Tm 41.6 0 Td 80.555556 Tz (30 ) Tj 30 0 0 30 0 1507 Tm 41.6 0 Td 77.777778 Tz (35 ) Tj 30 0 0 30 0 1299 Tm 41.566667 0 Td 83.333333 Tz (40 ) Tj 30 0 0 30 0 1091 Tm 41.566667 0 Td 80.555556 Tz (45 ) Tj 30 0 0 30 0 883 Tm 41.6 0 Td 80.555556 Tz (50 ) Tj 30 0 0 30 0 675 Tm 41.6 0 Td 77.777778 Tz (55 ) Tj 30 0 0 30 0 467 Tm 41.633333 0 Td 77.777778 Tz (60 ) Tj 30 0 0 30 0 259 Tm 41.633333 0 Td 75 Tz (65 ) Tj 51.428571 0 0 51.428571 0 2969 Tm 33.775 0 Td 77.777778 Tz (4 ) Tj 51.428571 0 0 51.428571 0 2916.604621 Tm 25.297222 0 Td 51.353276 Tz (administering ) Tj 4.297222 0 Td 55.092593 Tz (an ) Tj 0.972222 0 Td 46.450617 Tz (effective ) Tj 2.780556 0 Td 61.574074 Tz (amount ) Tj 2.508333 0 Td 56.712963 Tz (of ) Tj 0.875 0 Td 56.712963 Tz (an ) Tj 1.011111 0 Td 46.990741 Tz (injectable ) Tj 3.130556 0 Td 43.75 Tz (in ) Tj 0.836111 0 Td 43.75 Tz (situ ) Tj 51.428571 0 0 51.428571 0 2874.054254 Tm 25.297222 0 Td 51.851852 Tz (curing ) Tj 2.197222 0 Td 50.084175 Tz (biomaterial ) Tj 3.675 0 Td 52.175926 Tz (composite. ) Tj 3.461111 0 Td 63.734568 Tz (The ) Tj 1.477778 0 Td 50.378788 Tz (biomaterial ) Tj 3.675 0 Td 55.452675 Tz (composite ) Tj 51.428571 0 0 51.428571 0 2831.846154 Tm 25.297222 0 Td 53.240741 Tz (product ) Tj 2.372222 0 Td 37.268519 Tz (is ) Tj 0.583333 0 Td 53.06713 Tz (prepared ) Tj 2.663889 0 Td 59.953704 Tz (by ) Tj 0.836111 0 Td 58.333333 Tz (a ) Tj 0.466667 0 Td 51.388889 Tz (process ) Tj 2.313889 0 Td 50.771605 Tz (involving ) Tj 2.858333 0 Td 48.611111 Tz (the ) Tj 1.030556 0 Td 47.314815 Tz (steps ) Tj 1.575 0 Td 50.231481 Tz (of ) Tj 0.680556 0 Td 69.351852 Tz (admix ) Tj 51.428571 0 0 51.428571 0 2783.751979 Tm 25.297222 0 Td 50.771605 Tz (ing ) Tj 1.166667 0 Td 56.712963 Tz (an ) Tj 0.913889 0 Td 48.206019 Tz (alginate ) Tj 2.547222 0 Td 49.016204 Tz (solution ) Tj 2.586111 0 Td 53.472222 Tz (with ) Tj 1.536111 0 Td 43.75 Tz (at ) Tj 0.777778 0 Td 44.074074 Tz (least ) Tj 1.575 0 Td 57.253086 Tz (one ) Tj 1.283333 0 Td 53.703704 Tz (calcium ) Tj 2.527778 0 Td 39.537037 Tz (salt, ) Tj 1.438889 0 Td 45.37037 Tz (in ) Tj 0.797222 0 Td 55.092593 Tz (a ) Tj 51.428571 0 0 51.428571 0 2741.159334 Tm 25.316667 0 Td 44.722222 Tz (Sufficient ) Tj 2.8 0 Td 61.574074 Tz (amount ) Tj 2.333333 0 Td 46.990741 Tz (to ) Tj 0.758333 0 Td 41.724537 Tz (initiate ) Tj 2.119444 0 Td 52.546296 Tz (polymerization ) Tj 4.569444 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 48.611111 Tz (Solution ) Tj 2.488889 0 Td 46.990741 Tz (to ) Tj 51.428571 0 0 51.428571 0 2697.920635 Tm 25.297222 0 Td 58.333333 Tz (form ) Tj 1.536111 0 Td 58.333333 Tz (a ) Tj 0.466667 0 Td 51.49177 Tz (hydrogel, ) Tj 2.936111 0 Td 55.555556 Tz (wherein ) Tj 2.488889 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 53.703704 Tz (calcium ) Tj 2.430556 0 Td 41.319444 Tz (salt ) Tj 1.166667 0 Td 38.888889 Tz (is ) Tj 0.641667 0 Td 56.17284 Tz (nonporous ) Tj 3.325 0 Td 46.666667 Tz (B-tri ) Tj 51.428571 0 0 51.428571 0 2653.709518 Tm 25.297222 0 Td 54.166667 Tz (calcium ) Tj 2.508333 0 Td 51.851852 Tz (phosphate. ) Tj 3.344444 0 Td 63.734568 Tz (The ) Tj 1.361111 0 Td 52.777778 Tz (product ) Tj 2.430556 0 Td 56.17284 Tz (can ) Tj 1.225 0 Td 48.611111 Tz (also ) Tj 1.4 0 Td 50.925926 Tz (include ) Tj 2.333333 0 Td 58.333333 Tz (a ) Tj 0.563889 0 Td 55.092593 Tz (calcium ) Tj 51.428571 0 0 51.428571 0 2609.103118 Tm 25.316667 0 Td 38.888889 Tz (salt, ) Tj 1.302778 0 Td 54.282407 Tz (Such ) Tj 1.438889 0 Td 50.231481 Tz (as ) Tj 0.758333 0 Td 53.703704 Tz (calcium ) Tj 2.411111 0 Td 45.833333 Tz (Sulfate ) Tj 2.041667 0 Td 46.990741 Tz (to ) Tj 0.680556 0 Td 53.240741 Tz (provide ) Tj 2.333333 0 Td 56.712963 Tz (an ) Tj 0.797222 0 Td 48.611111 Tz (additional ) Tj 3.072222 0 Td 52.391975 Tz (source ) Tj 2.022222 0 Td 56.712963 Tz (of ) Tj 51.428571 0 0 51.428571 0 2567.407855 Tm 25.297222 0 Td 54.166667 Tz (calcium ) Tj 2.488889 0 Td 43.75 Tz (to ) Tj 0.738889 0 Td 49.691358 Tz (the ) Tj 1.069444 0 Td 50.771605 Tz (hydrogel. ) Tj 51.428571 0 0 51.428571 0 2522.37037 Tm 26.016667 0 Td 53.472222 Tz (This ) Tj 1.438889 0 Td 47.962963 Tz (invention, ) Tj 3.052778 0 Td 43.75 Tz (in ) Tj 0.661111 0 Td 53.240741 Tz (another ) Tj 2.352778 0 Td 57.744108 Tz (embodiment, ) Tj 3.947222 0 Td 51.851852 Tz (provides ) Tj 2.625 0 Td 58.333333 Tz (a ) Tj 0.486111 0 Td 51.851852 Tz (process ) Tj 51.428571 0 0 51.428571 0 2481.561983 Tm 25.277778 0 Td 46.990741 Tz (to ) Tj 0.7 0 Td 48.148148 Tz (release ) Tj 2.1 0 Td 58.333333 Tz (a ) Tj 0.447222 0 Td 48.971193 Tz (bioactive ) Tj 2.741667 0 Td 54.594017 Tz (agentoragents ) Tj 4.375 0 Td 46.990741 Tz (to ) Tj 0.680556 0 Td 55.092593 Tz (a ) Tj 0.427778 0 Td 46.180556 Tz (subject. ) Tj 2.372222 0 Td 50.231481 Tz (In ) Tj 0.7 0 Td 44.191919 Tz (particular, ) Tj 51.428571 0 0 51.428571 0 2436.843137 Tm 25.277778 0 Td 43.75 Tz (this ) Tj 1.263889 0 Td 51.851852 Tz (process ) Tj 2.430556 0 Td 49.421296 Tz (involves ) Tj 2.586111 0 Td 50.771605 Tz (the ) Tj 1.127778 0 Td 47.314815 Tz (steps ) Tj 1.652778 0 Td 58.333333 Tz (of ) Tj 0.797222 0 Td 51.602564 Tz (administering ) Tj 4.2 0 Td 58.333333 Tz (an ) Tj 0.933333 0 Td 46.450617 Tz (effective ) Tj 51.428571 0 0 51.428571 0 2392.783394 Tm 25.297222 0 Td 61.033951 Tz (amount ) Tj 2.352778 0 Td 58.333333 Tz (of ) Tj 0.758333 0 Td 59.953704 Tz (an ) Tj 0.894444 0 Td 46.990741 Tz (injectable ) Tj 2.994444 0 Td 45.37037 Tz (in ) Tj 0.738889 0 Td 42.939815 Tz (situ ) Tj 1.244444 0 Td 50.771605 Tz (curing ) Tj 1.983333 0 Td 50.378788 Tz (biomaterial ) Tj 3.519444 0 Td 55.452675 Tz (composite ) Tj 51.428571 0 0 51.428571 0 2351.134615 Tm 25.277778 0 Td 46.990741 Tz (to ) Tj 0.7 0 Td 50.771605 Tz (the ) Tj 1.030556 0 Td 46.180556 Tz (Subject. ) Tj 2.333333 0 Td 63.734568 Tz (The ) Tj 1.302778 0 Td 49.016204 Tz (material ) Tj 2.488889 0 Td 38.888889 Tz (is ) Tj 0.602778 0 Td 53.06713 Tz (prepared ) Tj 2.663889 0 Td 59.953704 Tz (by ) Tj 0.836111 0 Td 58.333333 Tz (a ) Tj 0.466667 0 Td 51.851852 Tz (process ) Tj 2.333333 0 Td 50.411523 Tz (involving ) Tj 51.428571 0 0 51.428571 0 2305.40404 Tm 25.277778 0 Td 50.771605 Tz (the ) Tj 1.166667 0 Td 47.314815 Tz (steps ) Tj 1.672222 0 Td 58.333333 Tz (of ) Tj 0.816667 0 Td 58.333333 Tz (admixing ) Tj 3.013889 0 Td 55.092593 Tz (an ) Tj 0.894444 0 Td 48.611111 Tz (alginate ) Tj 2.566667 0 Td 48.611111 Tz (Solution ) Tj 2.605556 0 Td 51.851852 Tz (with ) Tj 1.497222 0 Td 51.851852 Tz (a ) Tj 0.583333 0 Td 44.722222 Tz (sufficient ) Tj 51.428571 0 0 51.428571 0 2258.839949 Tm 25.297222 0 Td 61.033951 Tz (amount ) Tj 2.411111 0 Td 56.712963 Tz (of ) Tj 0.797222 0 Td 51.851852 Tz (a ) Tj 0.544444 0 Td 48.971193 Tz (bioactive ) Tj 2.838889 0 Td 53.148148 Tz (agent ) Tj 1.769444 0 Td 59.41358 Tz (and ) Tj 1.283333 0 Td 44.722222 Tz (least ) Tj 1.536111 0 Td 59.41358 Tz (one ) Tj 1.283333 0 Td 53.703704 Tz (calcium ) Tj 2.488889 0 Td 38.888889 Tz (salt, ) Tj 1.438889 0 Td 43.75 Tz (in ) Tj 0.758333 0 Td 55.092593 Tz (a ) Tj 51.428571 0 0 51.428571 0 2217.135231 Tm 25.316667 0 Td 44.722222 Tz (Sufficient ) Tj 2.780556 0 Td 61.033951 Tz (amount ) Tj 2.313889 0 Td 45.37037 Tz (to ) Tj 0.719444 0 Td 41.724537 Tz (initiate ) Tj 2.138889 0 Td 52.083333 Tz (polymerization ) Tj 4.530556 0 Td 53.472222 Tz (of ) Tj 0.719444 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 46.090535 Tz (Solution, ) Tj 2.625 0 Td 46.990741 Tz (to ) Tj 51.428571 0 0 51.428571 0 2174.137255 Tm 25.297222 0 Td 58.333333 Tz (form ) Tj 1.691667 0 Td 55.092593 Tz (a ) Tj 0.583333 0 Td 51.49177 Tz (hydrogel, ) Tj 3.091667 0 Td 55.092593 Tz (wherein ) Tj 2.605556 0 Td 49.691358 Tz (the ) Tj 1.166667 0 Td 54.166667 Tz (calcium ) Tj 2.586111 0 Td 42.939815 Tz (salt ) Tj 1.302778 0 Td 38.888889 Tz (is ) Tj 0.758333 0 Td 55.092593 Tz (a ) Tj 0.622222 0 Td 56.532922 Tz (nonporous ) Tj 51.428571 0 0 51.428571 0 2130.934247 Tm 25.433333 0 Td 47.800926 Tz (B-tricalcium ) Tj 4.219444 0 Td 51.527778 Tz (phosphate. ) Tj 3.869444 0 Td 62.654321 Tz (The ) Tj 1.905556 0 Td 54.012346 Tz (inflammatory ) Tj 4.705556 0 Td 55.092593 Tz (mediated ) Tj 51.428571 0 0 51.428571 0 2086.156863 Tm 25.297222 0 Td 53.06713 Tz (response ) Tj 2.741667 0 Td 49.907407 Tz (associated ) Tj 3.188889 0 Td 52.662037 Tz (with ) Tj 1.497222 0 Td 55.092593 Tz (a ) Tj 0.544444 0 Td 46.585648 Tz (fracture ) Tj 2.430556 0 Td 48.070988 Tz (repair ) Tj 1.905556 0 Td 55.092593 Tz (can ) Tj 1.186111 0 Td 58.333333 Tz (be ) Tj 0.913889 0 Td 56.893004 Tz (modulated ) Tj 51.428571 0 0 51.428571 0 2043.474227 Tm 25.277778 0 Td 61.574074 Tz (by ) Tj 0.933333 0 Td 55.497685 Tz (changing ) Tj 2.838889 0 Td 49.691358 Tz (the ) Tj 1.088889 0 Td 44.290123 Tz (ratios ) Tj 1.808333 0 Td 50.771605 Tz (within ) Tj 2.022222 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 50.084175 Tz (biomaterial ) Tj 3.5 0 Td 52.5 Tz (composite. ) Tj 51.428571 0 0 51.428571 0 1997.309645 Tm 26.036111 0 Td 48.611111 Tz (In ) Tj 0.797222 0 Td 52.314815 Tz (another ) Tj 2.372222 0 Td 57.744108 Tz (embodiment, ) Tj 4.005556 0 Td 43.75 Tz (this ) Tj 1.263889 0 Td 49.691358 Tz (invention ) Tj 2.877778 0 Td 52.256944 Tz (provides ) Tj 2.683333 0 Td 55.092593 Tz (a ) Tj 0.583333 0 Td 59.953704 Tz (method ) Tj 51.428571 0 0 51.428571 0 1954.45541 Tm 25.297222 0 Td 53.472222 Tz (of ) Tj 0.719444 0 Td 46.990741 Tz (treating ) Tj 2.333333 0 Td 58.333333 Tz (a ) Tj 0.466667 0 Td 50.411523 Tz (condition ) Tj 2.877778 0 Td 61.574074 Tz (whose ) Tj 1.944444 0 Td 45.833333 Tz (effects ) Tj 2.061111 0 Td 56.17284 Tz (can ) Tj 1.108333 0 Td 59.953704 Tz (be ) Tj 0.816667 0 Td 55.092593 Tz (reduced ) Tj 2.430556 0 Td 59.953704 Tz (by ) Tj 0.855556 0 Td 61.574074 Tz (stimu ) Tj 51.428571 0 0 51.428571 0 1912.8327 Tm 25.297222 0 Td 45.910494 Tz (lating ) Tj 1.963889 0 Td 49.691358 Tz (the ) Tj 1.225 0 Td 46.180556 Tz (cellular ) Tj 2.469444 0 Td 52.211934 Tz (responses ) Tj 3.15 0 Td 55.092593 Tz (induced ) Tj 2.605556 0 Td 59.953704 Tz (by ) Tj 1.05 0 Td 44.965278 Tz (platelet ) Tj 2.45 0 Td 47.530864 Tz (releasate ) Tj 51.428571 0 0 51.428571 0 1870.385542 Tm 25.297222 0 Td 51.49177 Tz (exposure. ) Tj 2.936111 0 Td 53.472222 Tz (This ) Tj 1.477778 0 Td 60.493827 Tz (method ) Tj 2.333333 0 Td 49.826389 Tz (involves ) Tj 2.566667 0 Td 51.353276 Tz (administering ) Tj 4.161111 0 Td 45.37037 Tz (to ) Tj 0.719444 0 Td 55.092593 Tz (a ) Tj 0.486111 0 Td 49.074074 Tz (Subject ) Tj 2.236111 0 Td 46.990741 Tz (in ) Tj 51.428571 0 0 51.428571 0 1824.26242 Tm 25.297222 0 Td 57.523148 Tz (need ) Tj 1.672222 0 Td 59.953704 Tz (of ) Tj 0.933333 0 Td 51.131687 Tz (treatment ) Tj 3.033333 0 Td 55.092593 Tz (a ) Tj 0.641667 0 Td 47.962963 Tz (therapeutically ) Tj 4.647222 0 Td 46.450617 Tz (effective ) Tj 2.780556 0 Td 61.574074 Tz (amount ) Tj 2.527778 0 Td 58.333333 Tz (of ) Tj 0.913889 0 Td 55.092593 Tz (a ) Tj 51.428571 0 0 51.428571 0 1781.368932 Tm 25.277778 0 Td 50.378788 Tz (biomaterial ) Tj 3.616667 0 Td 55.812757 Tz (composite ) Tj 3.305556 0 Td 51.851852 Tz (composition. ) Tj 4.025 0 Td 63.734568 Tz (The ) Tj 1.438889 0 Td 50.084175 Tz (biomaterial ) Tj 3.597222 0 Td 83.179012 Tz (com ) Tj 51.428571 0 0 51.428571 0 1739.169448 Tm 25.297222 0 Td 48.611111 Tz (posite ) Tj 2.002778 0 Td 54.208754 Tz (composition ) Tj 3.869444 0 Td 38.888889 Tz (is ) Tj 0.777778 0 Td 64.00463 Tz (made ) Tj 1.808333 0 Td 56.712963 Tz (of ) Tj 0.836111 0 Td 58.333333 Tz (a ) Tj 0.583333 0 Td 53.472222 Tz (hydrogel ) Tj 2.858333 0 Td 58.873457 Tz (formed ) Tj 2.372222 0 Td 57.523148 Tz (from ) Tj 1.652778 0 Td 59.953704 Tz (an ) Tj 51.428571 0 0 51.428571 0 1695.245922 Tm 25.297222 0 Td 48.206019 Tz (alginate ) Tj 2.586111 0 Td 50.771605 Tz (gel ) Tj 1.225 0 Td 54.552469 Tz (having ) Tj 2.275 0 Td 62.384259 Tz (embedded ) Tj 3.266667 0 Td 48.148148 Tz (therein ) Tj 2.372222 0 Td 56.17284 Tz (nonporous ) Tj 3.480556 0 Td 46.990741 Tz (B-trical ) Tj 52.857143 0 0 52.857143 0 1649.331695 Tm 24.613514 0 Td 57.545045 Tz (cium ) Tj 1.608108 0 Td 53.603604 Tz (phosphate ) Tj 3.083784 0 Td 46.036036 Tz (solid ) Tj 1.57027 0 Td 49.924925 Tz (phase, ) Tj 1.986486 0 Td 58.858859 Tz (and ) Tj 1.22973 0 Td 50.45045 Tz (a ) Tj 0.548649 0 Td 47.647648 Tz (bioactive ) Tj 2.743243 0 Td 47.822823 Tz (agent, ) Tj 1.948649 0 Td 54.504505 Tz (wherein ) Tj 51.428571 0 0 51.428571 0 1605.864686 Tm 25.277778 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 48.971193 Tz (bioactive ) Tj 2.819444 0 Td 53.796296 Tz (agent ) Tj 1.769444 0 Td 40.509259 Tz (is ) Tj 0.7 0 Td 50.925926 Tz (derived ) Tj 2.313889 0 Td 57.523148 Tz (from ) Tj 1.575 0 Td 43.425926 Tz (platelets. ) Tj 38.571429 0 0 38.571429 0 1530 Tm 36.633333 0 Td 90.740741 Tz (BRIEF ) Tj 3.007407 0 Td 94.668911 Tz (DESCRIPTION ) Tj 6.533333 0 Td 101.54321 Tz (OF ) Tj 1.451852 0 Td 105.144033 Tz (THE ) Tj 2.125926 0 Td 103.703704 Tz (SEVERAL ) Tj 38.571429 0 0 38.571429 0 1486 Tm 39.32963 0 Td 101.975309 Tz (VIEWS ) Tj 3.318519 0 Td 103.703704 Tz (OF ) Tj 1.451852 0 Td 106.584362 Tz (THE ) Tj 2.177778 0 Td 106.404321 Tz (DRAWINGS ) Tj 51.428571 0 0 51.428571 0 1393.008186 Tm 26.036111 0 Td 55.092593 Tz (FIG. ) Tj 1.652778 0 Td 48.611111 Tz (1 ) Tj 0.622222 0 Td 64.166667 Tz (Shows ) Tj 2.197222 0 Td 54.208754 Tz (composition ) Tj 3.947222 0 Td 35.648148 Tz (1 ) Tj 0.563889 0 Td 53.472222 Tz (with ) Tj 1.575 0 Td 54.444444 Tz (water ) Tj 1.886111 0 Td 47.43266 Tz (substituted ) Tj 3.441667 0 Td 48.611111 Tz (for ) Tj 51.428571 0 0 51.428571 0 1351.279953 Tm 25.297222 0 Td 51.58179 Tz (concentrated ) Tj 3.869444 0 Td 45.37037 Tz (platelet ) Tj 2.333333 0 Td 47.170782 Tz (releasate ) Tj 2.683333 0 Td 55.092593 Tz (being ) Tj 1.808333 0 Td 47.800926 Tz (injected ) Tj 2.508333 0 Td 56.712963 Tz (from ) Tj 1.633333 0 Td 55.092593 Tz (16-gauge ) Tj 51.428571 0 0 51.428571 0 1305.673054 Tm 25.297222 0 Td 52.932099 Tz (needle ) Tj 2.061111 0 Td 45.37037 Tz (in ) Tj 0.719444 0 Td 45.37037 Tz (air ) Tj 0.933333 0 Td 59.41358 Tz (and ) Tj 1.205556 0 Td 55.092593 Tz (phosphate ) Tj 3.111111 0 Td 51.041667 Tz (buffered ) Tj 2.625 0 Td 46.990741 Tz (saline ) Tj 1.847222 0 Td 58.333333 Tz (showing ) Tj 2.625 0 Td 55.555556 Tz (consoli ) Tj 51.428571 0 0 51.428571 0 1264.441379 Tm 25.297222 0 Td 50.231481 Tz (dation ) Tj 2.002778 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 50 Tz (ejected ) Tj 2.255556 0 Td 42.939815 Tz (gel. ) Tj 51.428571 0 0 51.428571 0 1219.505732 Tm 26.036111 0 Td 55.092593 Tz (FIG. ) Tj 1.497222 0 Td 58.333333 Tz (2 ) Tj 0.525 0 Td 59.62963 Tz (shows ) Tj 2.002778 0 Td 41.049383 Tz (rat ) Tj 0.913889 0 Td 59.806397 Tz (mesenchymal ) Tj 4.141667 0 Td 55.902778 Tz (stem ) Tj 1.497222 0 Td 42.939815 Tz (cell ) Tj 1.225 0 Td 66.759259 Tz (\(MCS\) ) Tj 2.177778 0 Td 50.771605 Tz (prolifera ) Tj 51.428571 0 0 51.428571 0 1174.453039 Tm 25.277778 0 Td 46.990741 Tz (tion ) Tj 1.438889 0 Td 58.333333 Tz (on ) Tj 1.011111 0 Td 58.333333 Tz (and ) Tj 1.322222 0 Td 56.712963 Tz (around ) Tj 2.391667 0 Td 32.407407 Tz (1 ) Tj 0.583333 0 Td 59.953704 Tz (ml ) Tj 1.011111 0 Td 49.421296 Tz (gels ) Tj 1.477778 0 Td 58.333333 Tz (and ) Tj 1.361111 0 Td 43.75 Tz (in ) Tj 0.836111 0 Td 57.973251 Tz (monolayer ) Tj 3.402778 0 Td 58.333333 Tz (on ) Tj 0.991667 0 Td 45.910494 Tz (tissue ) Tj 57.142857 0 0 57.142857 0 1132.002421 Tm 22.7675 0 Td 43.333333 Tz (culture ) Tj 1.96 0 Td 40.416667 Tz (plastic ) Tj 1.8375 0 Td 49.583333 Tz (\(TCP\). ) Tj 1.9425 0 Td 59.305556 Tz (Day ) Tj 1.225 0 Td 52.5 Tz (0 ) Tj 0.455 0 Td 44.333333 Tz (represents ) Tj 2.8 0 Td 53.083333 Tz (5x10" ) Tj 1.715 0 Td 40.25 Tz (cells ) Tj 1.33 0 Td 40.833333 Tz (at ) Tj 0.6125 0 Td 48.854167 Tz (time ) Tj 1.2775 0 Td 53.958333 Tz (of ) Tj 51.428571 0 0 51.428571 0 1093 Tm 25.316667 0 Td 49.016204 Tz (Seeding. ) Tj 48.571429 0 0 48.571429 0 1043 Tm 27.567647 0 Td 58.333333 Tz (FIG. ) Tj 1.914706 0 Td 60.906863 Tz (3A-I ) Tj 1.976471 0 Td 63.137255 Tz (shows ) Tj 2.429412 0 Td 49.754902 Tz (Sox. ) Tj 1.667647 0 Td 49.754902 Tz (9, ) Tj 1.152941 0 Td 61.19281 Tz (Runx2, ) Tj 2.717647 0 Td 52.900327 Tz (Osteocalcin, ) Tj 4.302941 0 Td 63.480392 Tz (ALP, ) Tj 51.428571 0 0 51.428571 0 1002.775904 Tm 25.297222 0 Td 50.771605 Tz (Osteonectin, ) Tj 3.85 0 Td 55.092593 Tz (Aggrecan, ) Tj 3.188889 0 Td 54.6875 Tz (Collagen ) Tj 2.858333 0 Td 34.027778 Tz (I, ) Tj 0.622222 0 Td 63.194444 Tz (CD105, ) Tj 2.488889 0 Td 58.333333 Tz (and ) Tj 1.302778 0 Td 52.5 Tz (Fibroblast ) Tj 51.428571 0 0 51.428571 0 961.173638 Tm 25.316667 0 Td 45.775463 Tz (specific ) Tj 2.45 0 Td 49.074074 Tz (Protein ) Tj 2.333333 0 Td 85.069444 Tz (mRNA ) Tj 2.255556 0 Td 49.421296 Tz (fold ) Tj 1.4 0 Td 57.253086 Tz (change ) Tj 2.275 0 Td 46.990741 Tz (in ) Tj 0.797222 0 Td 75.347222 Tz (MSCs ) Tj 2.041667 0 Td 60.925926 Tz (grown ) Tj 2.061111 0 Td 58.333333 Tz (on ) Tj 0.933333 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 921.47343 Tm 25.297222 0 Td 50.462963 Tz (various ) Tj 2.313889 0 Td 53.742284 Tz (compositions ) Tj 4.102778 0 Td 59.548611 Tz (compared ) Tj 3.033333 0 Td 46.990741 Tz (to ) Tj 0.758333 0 Td 56.17284 Tz (second ) Tj 2.216667 0 Td 54.166667 Tz (passage ) Tj 2.45 0 Td 76.157407 Tz (MSCs ) Tj 2.041667 0 Td 46.990741 Tz (in ) Tj 51.428571 0 0 51.428571 0 878.528846 Tm 25.297222 0 Td 58.333333 Tz (monolayer ) Tj 3.266667 0 Td 54.282407 Tz (over ) Tj 1.419444 0 Td 48.611111 Tz (time. ) Tj 1.691667 0 Td 49.537037 Tz (B-actin ) Tj 2.255556 0 Td 62.654321 Tz (was ) Tj 1.263889 0 Td 52.5 Tz (taken ) Tj 1.75 0 Td 50.231481 Tz (as ) Tj 0.777778 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 55.362654 Tz (housekeeping ) Tj 51.428571 0 0 51.428571 0 835.74092 Tm 25.297222 0 Td 51.851852 Tz (gene, ) Tj 1.711111 0 Td 59.41358 Tz (and ) Tj 1.244444 0 Td 59.806397 Tz (mesenchymal ) Tj 4.102778 0 Td 56.712963 Tz (stem ) Tj 1.516667 0 Td 44.074074 Tz (cells ) Tj 1.516667 0 Td 63.194444 Tz (\(MSCs\) ) Tj 2.411111 0 Td 46.990741 Tz (at ) Tj 0.7 0 Td 54.282407 Tz (time ) Tj 1.438889 0 Td 52.662037 Tz (Zero ) Tj 1.438889 0 Td 58.333333 Tz (were ) Tj 51.428571 0 0 51.428571 0 794.233129 Tm 25.277778 0 Td 52.5 Tz (taken ) Tj 1.769444 0 Td 50.231481 Tz (as ) Tj 0.797222 0 Td 49.826389 Tz (baseline ) Tj 2.566667 0 Td 49.151235 Tz (\(y=1\). ) Tj 51.428571 0 0 51.428571 0 751.035409 Tm 26.036111 0 Td 55.092593 Tz (FIG. ) Tj 1.458333 0 Td 61.574074 Tz (4 ) Tj 0.486111 0 Td 58.333333 Tz (shows ) Tj 1.886111 0 Td 53.148148 Tz (cumulative ) Tj 3.344444 0 Td 47.685185 Tz (release ) Tj 2.1 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 47.395833 Tz (Platelet-derived ) Tj 4.627778 0 Td 57.253086 Tz (growth ) Tj 51.428571 0 0 51.428571 0 709 Tm 25.297222 0 Td 46.990741 Tz (factor ) Tj 2.002778 0 Td 64.814815 Tz (\(PDGF\) ) Tj 2.605556 0 Td 48.611111 Tz (as ) Tj 0.933333 0 Td 32.407407 Tz (it ) Tj 0.7 0 Td 48.611111 Tz (diffused ) Tj 2.663889 0 Td 56.712963 Tz (from ) Tj 1.730556 0 Td 60.493827 Tz (F1+ ) Tj 1.419444 0 Td 58.333333 Tz (and ) Tj 1.380556 0 Td 54.282407 Tz (F2+. ) Tj 1.594444 0 Td 50.636574 Tz (Platelet ) Tj 51.428571 0 0 51.428571 0 668.044335 Tm 25.297222 0 Td 51.388889 Tz (derived ) Tj 2.313889 0 Td 57.253086 Tz (growth ) Tj 2.236111 0 Td 48.070988 Tz (factor ) Tj 1.886111 0 Td 64.814815 Tz (\(-BB ) Tj 1.75 0 Td 59.41358 Tz (and ) Tj 1.263889 0 Td 68.865741 Tz (-AB\) ) Tj 1.866667 0 Td 47.222222 Tz (release ) Tj 2.177778 0 Td 57.523148 Tz (from ) Tj 1.613889 0 Td 61.574074 Tz (F1+ ) Tj 1.283333 0 Td 60.493827 Tz (and ) Tj 51.428571 0 0 51.428571 0 629.20403 Tm 25.316667 0 Td 62.654321 Tz (F2+ ) Tj 1.361111 0 Td 61.574074 Tz (was ) Tj 1.361111 0 Td 50.462963 Tz (tracked ) Tj 2.313889 0 Td 48.611111 Tz (for ) Tj 1.05 0 Td 55.902778 Tz (each ) Tj 1.575 0 Td 56.712963 Tz (specimen ) Tj 2.975 0 Td 48.070988 Tz (individually ) Tj 3.694444 0 Td 54.444444 Tz (\(n=4\) ) Tj 1.827778 0 Td 43.75 Tz (at ) Tj 0.738889 0 Td 43.75 Tz (5, ) Tj 48.571429 0 0 48.571429 0 586 Tm 26.785294 0 Td 52.614379 Tz (24, ) Tj 1.173529 0 Td 52.614379 Tz (48, ) Tj 1.173529 0 Td 52.614379 Tz (72, ) Tj 1.152941 0 Td 61.764706 Tz (and ) Tj 1.379412 0 Td 61.764706 Tz (120 ) Tj 1.276471 0 Td 52.614379 Tz (hours. ) Tj 2.1 0 Td 56.221719 Tz (Concentration ) Tj 4.611765 0 Td 53.186275 Tz (values ) Tj 2.161765 0 Td 61.764706 Tz (were ) Tj 1.688235 0 Td 59.019608 Tz (deter ) Tj 51.428571 0 0 51.428571 0 544.881988 Tm 25.297222 0 Td 60.925926 Tz (mined ) Tj 2.002778 0 Td 53.703704 Tz (through ) Tj 2.488889 0 Td 60.493827 Tz (ELISA. ) Tj 51.428571 0 0 51.428571 0 502.551247 Tm 26.036111 0 Td 55.092593 Tz (FIG. ) Tj 1.633333 0 Td 61.574074 Tz (5 ) Tj 0.661111 0 Td 59.62963 Tz (shows ) Tj 2.1 0 Td 50.411523 Tz (depletion ) Tj 3.013889 0 Td 56.712963 Tz (of ) Tj 0.913889 0 Td 76.967593 Tz (PDGF ) Tj 2.177778 0 Td 56.712963 Tz (from ) Tj 1.691667 0 Td 46.296296 Tz (eluent. ) Tj 2.294444 0 Td 54.282407 Tz (Loss ) Tj 1.633333 0 Td 58.333333 Tz (of ) Tj 51.428571 0 0 51.428571 0 461.608905 Tm 25.316667 0 Td 54.484954 Tz (ELISA-detectable ) Tj 5.444444 0 Td 76.967593 Tz (PDGF ) Tj 2.061111 0 Td 50.771605 Tz (within ) Tj 2.041667 0 Td 48.611111 Tz (the ) Tj 1.069444 0 Td 52.391975 Tz (experimental ) Tj 4.005556 0 Td 49.907407 Tz (setup ) Tj 1.711111 0 Td 61.574074 Tz (was ) Tj 51.428571 0 0 51.428571 0 419.174684 Tm 25.297222 0 Td 55.452675 Tz (monitored ) Tj 3.227778 0 Td 53.703704 Tz (through ) Tj 2.527778 0 Td 48.611111 Tz (the ) Tj 1.108333 0 Td 49.421296 Tz (addition ) Tj 2.605556 0 Td 58.333333 Tz (of ) Tj 0.855556 0 Td 61.574074 Tz (400 ) Tj 1.361111 0 Td 64.814815 Tz (uL ) Tj 1.011111 0 Td 72.916667 Tz (hCPR ) Tj 1.983333 0 Td 45.37037 Tz (to ) Tj 0.797222 0 Td 66.111111 Tz (40-mL ) Tj 51.428571 0 0 51.428571 0 377.910891 Tm 25.297222 0 Td 49.691358 Tz (eluent ) Tj 1.886111 0 Td 60.493827 Tz (and ) Tj 1.205556 0 Td 57.87037 Tz (sampled ) Tj 2.547222 0 Td 45.37037 Tz (at ) Tj 0.661111 0 Td 54.282407 Tz (time ) Tj 1.438889 0 Td 47.314815 Tz (Zero, ) Tj 1.555556 0 Td 58.333333 Tz (5h, ) Tj 1.244444 0 Td 40.509259 Tz (1, ) Tj 0.622222 0 Td 43.75 Tz (2, ) Tj 0.661111 0 Td 45.37037 Tz (3, ) Tj 0.661111 0 Td 60.493827 Tz (and ) Tj 1.225 0 Td 55.092593 Tz (5 ) Tj 0.486111 0 Td 55.092593 Tz (days ) Tj 1.458333 0 Td 49.691358 Tz (\(n=3\). ) Tj 51.428571 0 0 51.428571 0 337.254717 Tm 25.297222 0 Td 63.734568 Tz (The ) Tj 1.283333 0 Td 55.092593 Tz (system ) Tj 2.138889 0 Td 54.552469 Tz (demonstrated ) Tj 4.044444 0 Td 51.851852 Tz (a ) Tj 0.427778 0 Td 49.583333 Tz (consistent ) Tj 3.091667 0 Td 49.331276 Tz (depletion ) Tj 2.819444 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 77.777778 Tz (PDGF ) Tj 1.983333 0 Td 52.932099 Tz (out ) Tj 51.428571 0 0 51.428571 0 294.787966 Tm 25.277778 0 Td 45.37037 Tz (to ) Tj 1.011111 0 Td 58.333333 Tz (72 ) Tj 1.127778 0 Td 49.691358 Tz (hours; ) Tj 2.236111 0 Td 42.939815 Tz (this ) Tj 1.458333 0 Td 46.990741 Tz (loss ) Tj 1.555556 0 Td 53.472222 Tz (with ) Tj 1.711111 0 Td 54.282407 Tz (time ) Tj 1.730556 0 Td 60.493827 Tz (was ) Tj 1.536111 0 Td 55.740741 Tz (found ) Tj 2.1 0 Td 45.959596 Tz (significant ) Tj 51.428571 0 0 51.428571 0 253.814994 Tm 25.316667 0 Td 51.557239 Tz (\(p<0.0001\). ) Tj 3.538889 0 Td 59.953704 Tz (At ) Tj 0.913889 0 Td 57.253086 Tz (120 ) Tj 1.127778 0 Td 53.148148 Tz (hours ) Tj 1.75 0 Td 58.333333 Tz (an ) Tj 0.816667 0 Td 53.703704 Tz (average ) Tj 2.411111 0 Td 71.296296 Tz (65% ) Tj 1.419444 0 Td 56.712963 Tz (of ) Tj 0.777778 0 Td 49.691358 Tz (the ) Tj 0.991667 0 Td 40.277778 Tz (initial ) Tj 1.847222 0 Td 76.157407 Tz (PDGF ) Tj 51.428571 0 0 51.428571 0 211.200517 Tm 25.277778 0 Td 62.654321 Tz (was ) Tj 1.438889 0 Td 49.109687 Tz (undetectable. ) Tj 4.2 0 Td 55.902778 Tz (Data ) Tj 1.594444 0 Td 50.771605 Tz (are ) Tj 1.225 0 Td 61.574074 Tz (shown ) Tj 2.158333 0 Td 50.231481 Tz (as ) Tj 0.894444 0 Td 53.703704 Tz (average ) Tj 2.566667 0 Td 50.771605 Tz (values ) Tj 2.119444 0 Td 53.472222 Tz (with ) Tj 51.428571 0 0 51.428571 0 169.721785 Tm 25.297222 0 Td 47.962963 Tz (error ) Tj 1.594444 0 Td 50.231481 Tz (bars ) Tj 1.419444 0 Td 48.611111 Tz (indicated ) Tj 2.819444 0 Td 53.148148 Tz (plus/minus ) Tj 3.402778 0 Td 57.253086 Tz (one ) Tj 1.205556 0 Td 51.446759 Tz (standard ) Tj 2.644444 0 Td 47.638889 Tz (deviation. ) Tj ET Q endstream endobj 454 0 obj << /Length 1 >> stream q endstream endobj 455 0 obj << /Length 48442 >> stream Q q 0.24 0 0 0.24 0 0 cm BT /F1 1 Tf 3 Tr 62.857143 0 0 62.857143 0 3023 Tm 17.325 0 Td 83.522727 Tz (US ) Tj 1.240909 0 Td 58.333333 Tz (8,404,256 ) Tj 3.388636 0 Td 74.242424 Tz (B2 ) Tj 51.428571 0 0 51.428571 0 2968 Tm 14.875 0 Td 74.537037 Tz (5 ) Tj 51.428571 0 0 51.428571 0 2914.013942 Tm 7.116667 0 Td 54.282407 Tz (FIG. ) Tj 1.497222 0 Td 71.296296 Tz (6A ) Tj 1.030556 0 Td 58.981481 Tz (shows ) Tj 1.944444 0 Td 54.012346 Tz (simple ) Tj 2.061111 0 Td 55.092593 Tz (chemotaxis ) Tj 3.5 0 Td 44.444444 Tz (results ) Tj 2.022222 0 Td 53.472222 Tz (of ) Tj 0.719444 0 Td 57.79321 Tz (TIB-71 ) Tj 2.333333 0 Td 87.5 Tz (RAW ) Tj 51.428571 0 0 51.428571 0 2874.634615 Tm 6.358333 0 Td 54.444444 Tz (264.7 ) Tj 1.827778 0 Td 57.253086 Tz (monocytes ) Tj 3.266667 0 Td 52.662037 Tz (with ) Tj 1.419444 0 Td 53.240741 Tz (average ) Tj 2.430556 0 Td 49.691358 Tz (values ) Tj 1.944444 0 Td 53.472222 Tz (with ) Tj 1.419444 0 Td 48.611111 Tz (error ) Tj 1.555556 0 Td 51.041667 Tz (bars ) Tj 1.4 0 Td 52.314815 Tz (indicat ) Tj 51.428571 0 0 51.428571 0 2833.137546 Tm 6.358333 0 Td 50.771605 Tz (ing ) Tj 1.108333 0 Td 53.148148 Tz (plus/minus ) Tj 3.402778 0 Td 57.253086 Tz (one ) Tj 1.205556 0 Td 51.446759 Tz (standard ) Tj 2.644444 0 Td 47.638889 Tz (deviation. ) Tj 51.428571 0 0 51.428571 0 2788 Tm 7.116667 0 Td 54.282407 Tz (FIG. ) Tj 1.477778 0 Td 66.435185 Tz (6B ) Tj 0.952778 0 Td 58.981481 Tz (shows ) Tj 1.905556 0 Td 65.462963 Tz (human ) Tj 2.1 0 Td 50.771605 Tz (umbilical ) Tj 2.897222 0 Td 48.611111 Tz (vein ) Tj 1.322222 0 Td 48.905724 Tz (endothelial ) Tj 3.344444 0 Td 43.75 Tz (cell ) Tj 1.166667 0 Td 85.339506 Tz (\(HU ) Tj 51.428571 0 0 51.428571 0 2748.914174 Tm 6.358333 0 Td 71.296296 Tz (VEC\) ) Tj 2.119444 0 Td 48.179012 Tz (tranendothelial ) Tj 4.783333 0 Td 52.211934 Tz (migration ) Tj 3.188889 0 Td 63.194444 Tz (by ) Tj 1.127778 0 Td 57.79321 Tz (TIB-71 ) Tj 2.586111 0 Td 88.580247 Tz (RAW ) Tj 2.002778 0 Td 55.092593 Tz (264.7 ) Tj 51.428571 0 0 51.428571 0 2709.340534 Tm 6.358333 0 Td 57.973251 Tz (monocytes ) Tj 3.461111 0 Td 52.662037 Tz (with ) Tj 1.575 0 Td 53.703704 Tz (average ) Tj 2.586111 0 Td 49.691358 Tz (values ) Tj 2.119444 0 Td 52.662037 Tz (with ) Tj 1.575 0 Td 48.611111 Tz (error ) Tj 1.711111 0 Td 51.041667 Tz (bars ) Tj 1.536111 0 Td 48.611111 Tz (indicating ) Tj 51.428571 0 0 51.428571 0 2666.666667 Tm 6.358333 0 Td 53.148148 Tz (plus/minus ) Tj 3.383333 0 Td 58.333333 Tz (one ) Tj 1.225 0 Td 51.446759 Tz (standard ) Tj 2.644444 0 Td 47.638889 Tz (deviation. ) Tj 51.428571 0 0 51.428571 0 2624.602667 Tm 7.116667 0 Td 54.282407 Tz (FIG. ) Tj 1.613889 0 Td 71.296296 Tz (7A ) Tj 1.127778 0 Td 59.62963 Tz (shows ) Tj 2.061111 0 Td 49.691358 Tz (the ) Tj 1.147222 0 Td 54.012346 Tz (simple ) Tj 2.216667 0 Td 51.851852 Tz (migration ) Tj 3.072222 0 Td 59.953704 Tz (of ) Tj 0.875 0 Td 69.907407 Tz (HUVECs. ) Tj 3.266667 0 Td 55.902778 Tz (Data ) Tj 51.428571 0 0 51.428571 0 2583.679426 Tm 6.377778 0 Td 59.953704 Tz (show ) Tj 1.633333 0 Td 53.240741 Tz (average ) Tj 2.411111 0 Td 49.691358 Tz (values ) Tj 1.925 0 Td 53.472222 Tz (with ) Tj 1.438889 0 Td 47.314815 Tz (error ) Tj 1.536111 0 Td 49.421296 Tz (bars ) Tj 1.361111 0 Td 48.971193 Tz (indicated ) Tj 2.741667 0 Td 53.472222 Tz (plus/minus ) Tj 3.363889 0 Td 58.333333 Tz (one ) Tj 40 0 0 40 0 2547 Tm 8.2 0 Td 65.625 Tz (standard ) Tj 3.4 0 Td 60.833333 Tz (deviation. ) Tj 51.428571 0 0 51.428571 0 2500.513648 Tm 7.116667 0 Td 54.282407 Tz (FIG. ) Tj 1.477778 0 Td 66.435185 Tz (7B ) Tj 0.991667 0 Td 43.981481 Tz (\(right\) ) Tj 2.022222 0 Td 58.981481 Tz (shows ) Tj 1.925 0 Td 52.459491 Tz (enzymatic-driven ) Tj 5.230556 0 Td 82.962963 Tz (HUVEC ) Tj 2.663889 0 Td 50.636574 Tz (invasion ) Tj 51.428571 0 0 51.428571 0 2459.575309 Tm 6.377778 0 Td 47.800926 Tz (\(haptotaxis\) ) Tj 3.733333 0 Td 53.703704 Tz (through ) Tj 2.527778 0 Td 57.928241 Tz (basement ) Tj 3.052778 0 Td 64.00463 Tz (membrane ) Tj 3.325 0 Td 44.722222 Tz (gels. ) Tj 1.672222 0 Td 55.902778 Tz (Data ) Tj 1.613889 0 Td 63.194444 Tz (show ) Tj 51.428571 0 0 51.428571 0 2418.051948 Tm 6.358333 0 Td 53.703704 Tz (average ) Tj 2.391667 0 Td 49.691358 Tz (values ) Tj 1.925 0 Td 52.662037 Tz (with ) Tj 1.4 0 Td 47.962963 Tz (error ) Tj 1.516667 0 Td 50.231481 Tz (bars ) Tj 1.322222 0 Td 48.611111 Tz (indicating ) Tj 3.033333 0 Td 52.824074 Tz (plus/minus ) Tj 3.305556 0 Td 58.333333 Tz (one ) Tj 1.186111 0 Td 57.523148 Tz (stan ) Tj 40 0 0 40 0 2381 Tm 8.175 0 Td 69.791667 Tz (dard ) Tj 1.9 0 Td 60.833333 Tz (deviation. ) Tj 51.428571 0 0 51.428571 0 2334.153061 Tm 7.116667 0 Td 54.282407 Tz (FIG. ) Tj 1.477778 0 Td 76.157407 Tz (8A ) Tj 1.088889 0 Td 59.62963 Tz (shows ) Tj 1.983333 0 Td 43.75 Tz (in ) Tj 0.758333 0 Td 44.074074 Tz (vitro ) Tj 1.516667 0 Td 51.851852 Tz (angiogenesis ) Tj 3.908333 0 Td 51.851852 Tz (assessed ) Tj 2.625 0 Td 54.166667 Tz (through ) Tj 2.469444 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 2293.589311 Tm 6.358333 0 Td 43.981481 Tz (ability ) Tj 2.391667 0 Td 58.333333 Tz (and ) Tj 1.594444 0 Td 49.151235 Tz (extent ) Tj 2.294444 0 Td 59.953704 Tz (of ) Tj 1.147222 0 Td 54.503367 Tz (spontaneous ) Tj 4.141667 0 Td 51.851852 Tz (tube ) Tj 1.788889 0 Td 52.572016 Tz (formation ) Tj 3.363889 0 Td 63.194444 Tz (by ) Tj 38.571429 0 0 38.571429 0 2256 Tm 8.52963 0 Td 92.592593 Tz (HUVECS. ) Tj 51.428571 0 0 51.428571 0 2209.167305 Tm 7.116667 0 Td 54.282407 Tz (FIG. ) Tj 1.477778 0 Td 71.296296 Tz (8B ) Tj 1.05 0 Td 60.277778 Tz (shows ) Tj 2.002778 0 Td 43.75 Tz (in ) Tj 0.758333 0 Td 44.074074 Tz (vitro ) Tj 1.497222 0 Td 52.121914 Tz (angiogenesis ) Tj 3.927778 0 Td 51.851852 Tz (assessed ) Tj 2.644444 0 Td 54.166667 Tz (through ) Tj 2.469444 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 2168.589311 Tm 6.358333 0 Td 43.981481 Tz (ability ) Tj 2.391667 0 Td 58.333333 Tz (and ) Tj 1.594444 0 Td 49.151235 Tz (extent ) Tj 2.294444 0 Td 59.953704 Tz (of ) Tj 1.147222 0 Td 54.503367 Tz (spontaneous ) Tj 4.141667 0 Td 51.851852 Tz (tube ) Tj 1.788889 0 Td 52.572016 Tz (formation ) Tj 3.363889 0 Td 63.194444 Tz (by ) Tj 38.571429 0 0 38.571429 0 2131 Tm 8.52963 0 Td 92.592593 Tz (HUVECS. ) Tj 51.428571 0 0 51.428571 0 2085.163265 Tm 7.116667 0 Td 54.282407 Tz (FIG. ) Tj 1.477778 0 Td 72.916667 Tz (8C ) Tj 1.05 0 Td 60.277778 Tz (shows ) Tj 2.002778 0 Td 43.75 Tz (in ) Tj 0.758333 0 Td 44.074074 Tz (vitro ) Tj 1.497222 0 Td 52.121914 Tz (angiogenesis ) Tj 3.927778 0 Td 51.851852 Tz (assessed ) Tj 2.644444 0 Td 54.166667 Tz (through ) Tj 2.469444 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 2043.589311 Tm 6.358333 0 Td 43.981481 Tz (ability ) Tj 2.391667 0 Td 58.333333 Tz (and ) Tj 1.594444 0 Td 49.151235 Tz (extent ) Tj 2.294444 0 Td 59.953704 Tz (of ) Tj 1.147222 0 Td 54.503367 Tz (spontaneous ) Tj 4.141667 0 Td 51.851852 Tz (tube ) Tj 1.788889 0 Td 52.572016 Tz (formation ) Tj 3.363889 0 Td 63.194444 Tz (by ) Tj 38.571429 0 0 38.571429 0 2007 Tm 8.52963 0 Td 92.592593 Tz (HUVECS. ) Tj 51.428571 0 0 51.428571 0 1959.277854 Tm 7.116667 0 Td 54.282407 Tz (FIG. ) Tj 1.652778 0 Td 74.537037 Tz (8D ) Tj 1.244444 0 Td 58.333333 Tz (shows ) Tj 2.138889 0 Td 84.259259 Tz (D ) Tj 0.875 0 Td 47.916667 Tz (representative ) Tj 4.375 0 Td 58.333333 Tz (F ) Tj 0.427778 0 Td 59.953704 Tz (1b ) Tj 1.069444 0 Td 53.472222 Tz (tube ) Tj 1.594444 0 Td 50.084175 Tz (formations, ) Tj 52.857143 0 0 52.857143 0 1915 Tm 6.167568 0 Td 58.018018 Tz (which ) Tj 1.92973 0 Td 57.545045 Tz (were ) Tj 1.551351 0 Td 47.297297 Tz (similar ) Tj 2.137838 0 Td 45.720721 Tz (to ) Tj 0.775676 0 Td 59.121622 Tz (F2b. ) Tj 51.428571 0 0 51.428571 0 1876.054591 Tm 7.116667 0 Td 59.259259 Tz (FIG.8E: ) Tj 2.722222 0 Td 58.981481 Tz (shows ) Tj 1.983333 0 Td 47.916667 Tz (representative ) Tj 4.219444 0 Td 62.037037 Tz (F1-tube ) Tj 2.8 0 Td 50.084175 Tz (formations, ) Tj 3.5 0 Td 61.574074 Tz (simi ) Tj 40 0 0 40 0 1840 Tm 8.175 0 Td 56.944444 Tz (lar ) Tj 1.225 0 Td 56.25 Tz (to ) Tj 0.975 0 Td 81.944444 Tz (F2- ) Tj 1.725 0 Td 75 Tz (and ) Tj 1.6 0 Td 93.75 Tz (M200 ) Tj 2.5 0 Td 76.785714 Tz (medium. ) Tj 51.428571 0 0 51.428571 0 1794.887179 Tm 7.116667 0 Td 54.282407 Tz (FIG. ) Tj 1.536111 0 Td 64.814815 Tz (8F ) Tj 0.991667 0 Td 59.62963 Tz (shows ) Tj 2.041667 0 Td 59.143519 Tz (BBE-supplemented ) Tj 5.872222 0 Td 72.106481 Tz (M200 ) Tj 1.944444 0 Td 56.388889 Tz (group ) Tj 1.905556 0 Td 54.282407 Tz (with ) Tj 1.536111 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 1752.019417 Tm 6.377778 0 Td 50 Tz (Image.J ) Tj 2.255556 0 Td 50 Tz (created ) Tj 2.275 0 Td 50.462963 Tz (overlay ) Tj 2.313889 0 Td 56.712963 Tz (of ) Tj 0.738889 0 Td 50.771605 Tz (the ) Tj 1.05 0 Td 55.632716 Tz (branch ) Tj 2.177778 0 Td 49.826389 Tz (skeleton ) Tj 2.586111 0 Td 55.092593 Tz (used ) Tj 1.458333 0 Td 46.990741 Tz (to ) Tj 0.758333 0 Td 60.925926 Tz (gener ) Tj 51.428571 0 0 51.428571 0 1710.012072 Tm 6.358333 0 Td 47.530864 Tz (ate ) Tj 1.011111 0 Td 50.771605 Tz (the ) Tj 1.108333 0 Td 57.253086 Tz (raw ) Tj 1.225 0 Td 55.092593 Tz (branch ) Tj 2.158333 0 Td 60.493827 Tz (and ) Tj 1.205556 0 Td 50.636574 Tz (junction ) Tj 2.625 0 Td 45.37037 Tz (data. ) Tj 51.428571 0 0 51.428571 0 1667.714286 Tm 7.116667 0 Td 54.282407 Tz (FIG. ) Tj 1.497222 0 Td 64.814815 Tz (9 ) Tj 0.622222 0 Td 59.62963 Tz (shows ) Tj 2.022222 0 Td 49.421296 Tz (data ) Tj 1.419444 0 Td 47.530864 Tz (for ) Tj 1.05 0 Td 89.12037 Tz (NO ) Tj 1.244444 0 Td 52.5 Tz (production ) Tj 3.422222 0 Td 55.092593 Tz (measures ) Tj 2.877778 0 Td 48.611111 Tz (as ) Tj 0.836111 0 Td 41.203704 Tz (nitrite ) Tj 52.857143 0 0 52.857143 0 1624.463659 Tm 6.186486 0 Td 52.815315 Tz (accumulation ) Tj 4.124324 0 Td 44.144144 Tz (in ) Tj 0.813514 0 Td 55.705706 Tz (TIB-71 ) Tj 2.383784 0 Td 65.822072 Tz (RAW264.7 ) Tj 3.481081 0 Td 60.540541 Tz (mouse ) Tj 2.118919 0 Td 57.545045 Tz (monocyte ) Tj 3.045946 0 Td 42.567568 Tz (cell ) Tj 40 0 0 40 0 1590 Tm 8.175 0 Td 59.375 Tz (line ) Tj 1.6 0 Td 61.458333 Tz (cultures ) Tj 3.125 0 Td 65.277778 Tz (are ) Tj 1.375 0 Td 80 Tz (shown ) Tj 2.6 0 Td 57.8125 Tz (relative ) Tj 3 0 Td 56.25 Tz (to ) Tj 0.9 0 Td 63.888889 Tz (the ) Tj 1.3 0 Td 65 Tz (basal ) Tj 2.15 0 Td 69.791667 Tz (time ) Tj 1.875 0 Td 65.625 Tz (Zero ) Tj 1.8 0 Td 58.333333 Tz (level ) Tj 1.975 0 Td 72.916667 Tz (of ) Tj 51.428571 0 0 51.428571 0 1540.912189 Tm 6.358333 0 Td 52.314815 Tz (3.5+0.9 ) Tj 2.508333 0 Td 81.018519 Tz (uM ) Tj 1.244444 0 Td 41.203704 Tz (nitrite ) Tj 1.963889 0 Td 59.41358 Tz (and ) Tj 1.322222 0 Td 49.331276 Tz (indicated ) Tj 2.897222 0 Td 46.990741 Tz (in ) Tj 0.816667 0 Td 50.771605 Tz (the ) Tj 1.166667 0 Td 46.990741 Tz (figure ) Tj 1.925 0 Td 45.37037 Tz (at ) Tj 0.797222 0 Td 48.611111 Tz (the ) Tj 1.205556 0 Td 67.24537 Tz (100% ) Tj 51.428571 0 0 51.428571 0 1501.773964 Tm 6.358333 0 Td 47.530864 Tz (value. ) Tj 1.866667 0 Td 62.037037 Tz (Columns ) Tj 2.741667 0 Td 61.574074 Tz (show ) Tj 1.613889 0 Td 53.703704 Tz (average ) Tj 2.352778 0 Td 52.146465 Tz (percentages ) Tj 3.577778 0 Td 58.333333 Tz (and ) Tj 1.147222 0 Td 51.041667 Tz (bars ) Tj 1.341667 0 Td 48.206019 Tz (indicate ) Tj 2.469444 0 Td 29.166667 Tz (it ) Tj 51.428571 0 0 51.428571 0 1459.826347 Tm 6.358333 0 Td 59.41358 Tz (one ) Tj 1.205556 0 Td 51.446759 Tz (standard ) Tj 2.625 0 Td 47.314815 Tz (deviation. ) Tj 3.052778 0 Td 55.902778 Tz (Data ) Tj 1.497222 0 Td 59.143519 Tz (were ) Tj 1.555556 0 Td 48.341049 Tz (investigated ) Tj 3.616667 0 Td 53.703704 Tz (through ) Tj 2.411111 0 Td 58.333333 Tz (a ) Tj 0.486111 0 Td 42.12963 Tz (full ) Tj 51.428571 0 0 51.428571 0 1417.17561 Tm 6.358333 0 Td 44.650206 Tz (factorial ) Tj 2.605556 0 Td 59.722222 Tz (two-way ) Tj 2.683333 0 Td 82.314815 Tz (ANOVA ) Tj 2.663889 0 Td 58.333333 Tz (and ) Tj 1.186111 0 Td 60.925926 Tz (Tukey ) Tj 2.022222 0 Td 42.12963 Tz (tests ) Tj 1.477778 0 Td 50.231481 Tz (\(a=0.05, ) Tj 2.625 0 Td 47.530864 Tz (for ) Tj 0.991667 0 Td 38.240741 Tz (all\). ) Tj 51.428571 0 0 51.428571 0 1376.594315 Tm 7.116667 0 Td 54.282407 Tz (FIG. ) Tj 1.516667 0 Td 58.333333 Tz (10 ) Tj 0.894444 0 Td 59.62963 Tz (shows ) Tj 1.983333 0 Td 54.62963 Tz (reduced ) Tj 2.469444 0 Td 66.666667 Tz (qRT-PCR ) Tj 2.975 0 Td 49.421296 Tz (data ) Tj 1.4 0 Td 48.611111 Tz (for ) Tj 1.011111 0 Td 48.611111 Tz (the ) Tj 1.069444 0 Td 47.800926 Tz (fold ) Tj 1.322222 0 Td 57.79321 Tz (change ) Tj 51.428571 0 0 51.428571 0 1334.909091 Tm 6.358333 0 Td 69.675926 Tz (iNOS ) Tj 1.788889 0 Td 51.851852 Tz (expression ) Tj 3.266667 0 Td 45.37037 Tz (in ) Tj 0.641667 0 Td 57.253086 Tz (TIB-71 ) Tj 2.313889 0 Td 67.24537 Tz (RAW264.7 ) Tj 3.383333 0 Td 64.166667 Tz (mouse ) Tj 2.041667 0 Td 59.953704 Tz (monocyte ) Tj 2.975 0 Td 43.75 Tz (cell ) Tj 51.428571 0 0 51.428571 0 1292.301435 Tm 6.358333 0 Td 46.180556 Tz (line ) Tj 1.205556 0 Td 47.800926 Tz (cultures ) Tj 2.411111 0 Td 50.231481 Tz (as ) Tj 0.738889 0 Td 51.851852 Tz (a ) Tj 0.466667 0 Td 43.75 Tz (result ) Tj 1.711111 0 Td 53.472222 Tz (of ) Tj 0.719444 0 Td 49.016204 Tz (material ) Tj 2.488889 0 Td 51.851852 Tz (or ) Tj 0.758333 0 Td 66.975309 Tz (LPS ) Tj 1.322222 0 Td 53.652263 Tz (endotoxin ) Tj 3.013889 0 Td 55.092593 Tz (exposure ) Tj 51.428571 0 0 51.428571 0 1251.496084 Tm 6.358333 0 Td 54.282407 Tz (over ) Tj 1.594444 0 Td 53.472222 Tz (time ) Tj 1.613889 0 Td 38.888889 Tz (is ) Tj 0.797222 0 Td 56.17284 Tz (shown. ) Tj 2.391667 0 Td 55.092593 Tz (Data ) Tj 1.652778 0 Td 50.771605 Tz (reduction ) Tj 3.072222 0 Td 61.574074 Tz (was ) Tj 1.4 0 Td 56.532922 Tz (performed ) Tj 3.363889 0 Td 53.148148 Tz (using ) Tj 52.857143 0 0 52.857143 0 1208.194614 Tm 6.186486 0 Td 75.675676 Tz (AAC ) Tj 1.797297 0 Td 48.085586 Tz (analysis ) Tj 2.478378 0 Td 48.698699 Tz (\(standard ) Tj 2.8 0 Td 66.216216 Tz (AAC, ) Tj 1.816216 0 Td 52.027027 Tz (with ) Tj 1.418919 0 Td 49.399399 Tz (the ) Tj 1.040541 0 Td 47.297297 Tz (additional ) Tj 3.045946 0 Td 53.997748 Tz (consider ) Tj 51.428571 0 0 51.428571 0 1170.21957 Tm 6.358333 0 Td 47.962963 Tz (ation ) Tj 1.652778 0 Td 54.552469 Tz (primer ) Tj 2.1 0 Td 49.421296 Tz (pair ) Tj 1.341667 0 Td 45.100309 Tz (efficiency\). ) Tj 3.422222 0 Td 83.611111 Tz (ANOVA ) Tj 2.683333 0 Td 54.6875 Tz (\(C-0.05\) ) Tj 2.819444 0 Td 62.654321 Tz (was ) Tj 1.283333 0 Td 52.314815 Tz (applied ) Tj 51.428571 0 0 51.428571 0 1126.382134 Tm 6.338889 0 Td 46.990741 Tz (to ) Tj 0.758333 0 Td 58.333333 Tz (a ) Tj 0.544444 0 Td 52.932099 Tz (second-order ) Tj 4.005556 0 Td 53.06713 Tz (response ) Tj 2.722222 0 Td 50 Tz (Surface ) Tj 2.333333 0 Td 58.981481 Tz (model ) Tj 2.002778 0 Td 43.75 Tz (in ) Tj 0.738889 0 Td 50.462963 Tz (regards ) Tj 2.313889 0 Td 46.990741 Tz (to ) Tj 0.758333 0 Td 55.092593 Tz (time ) Tj 51.428571 0 0 51.428571 0 1083 Tm 6.338889 0 Td 54.282407 Tz (with ) Tj 1.458333 0 Td 51.131687 Tz (treatment ) Tj 2.877778 0 Td 51.851852 Tz (as ) Tj 0.777778 0 Td 55.092593 Tz (a ) Tj 0.486111 0 Td 46.450617 Tz (linear ) Tj 1.808333 0 Td 48.611111 Tz (covariate ) Tj 2.761111 0 Td 52.121914 Tz (onlinearized ) Tj 3.908333 0 Td 79.398148 Tz (AC ) Tj 1.4 0 Td 47.222222 Tz (values. ) Tj 51.428571 0 0 51.428571 0 1042.893671 Tm 7.116667 0 Td 54.282407 Tz (FIG. ) Tj 1.497222 0 Td 48.611111 Tz (11. ) Tj 1.069444 0 Td 63.518519 Tz (Shows ) Tj 2.1 0 Td 69.135802 Tz (uCT ) Tj 1.438889 0 Td 50.501543 Tz (radiographic ) Tj 3.811111 0 Td 54.552469 Tz (\(a.and ) Tj 2.119444 0 Td 43.209877 Tz (b.\) ) Tj 0.894444 0 Td 60.493827 Tz (and ) Tj 1.244444 0 Td 53.472222 Tz (rendered ) Tj 51.428571 0 0 51.428571 0 1002.308261 Tm 6.377778 0 Td 40.509259 Tz (\(c.\) ) Tj 1.147222 0 Td 56.17284 Tz (images ) Tj 2.177778 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 46.8107 Tz (injection ) Tj 2.683333 0 Td 50.05144 Tz (delivered ) Tj 2.819444 0 Td 59.41358 Tz (and ) Tj 1.225 0 Td 45.37037 Tz (in ) Tj 0.7 0 Td 43.75 Tz (situ ) Tj 1.225 0 Td 53.148148 Tz (cured ) Tj 1.788889 0 Td 53.472222 Tz (F1 ) Tj 0.738889 0 Td 58.333333 Tz (+ ) Tj 0.563889 0 Td 67.24537 Tz (mate ) Tj 51.428571 0 0 51.428571 0 958.994652 Tm 6.358333 0 Td 40.509259 Tz (rial ) Tj 1.302778 0 Td 50.771605 Tz (within ) Tj 2.177778 0 Td 48.611111 Tz (the ) Tj 1.186111 0 Td 50.87963 Tz (cancellous ) Tj 3.422222 0 Td 50.231481 Tz (region ) Tj 2.119444 0 Td 59.953704 Tz (of ) Tj 0.933333 0 Td 51.851852 Tz (a ) Tj 0.661111 0 Td 42.12963 Tz (rat ) Tj 1.05 0 Td 58.333333 Tz (Ca-2 ) Tj 1.769444 0 Td 46.990741 Tz (vertebrae. ) Tj 51.428571 0 0 51.428571 0 919.75153 Tm 6.377778 0 Td 50.501543 Tz (B-tricalcium ) Tj 3.830556 0 Td 55.092593 Tz (phosphate ) Tj 3.130556 0 Td 51.851852 Tz (granules ) Tj 2.683333 0 Td 55.092593 Tz (can ) Tj 1.186111 0 Td 58.333333 Tz (be ) Tj 0.875 0 Td 52.662037 Tz (seen ) Tj 1.497222 0 Td 48.611111 Tz (as ) Tj 0.777778 0 Td 49.691358 Tz (the ) Tj 1.108333 0 Td 49.016204 Tz (material ) Tj 51.428571 0 0 51.428571 0 878.707317 Tm 6.338889 0 Td 54.282407 Tz (with ) Tj 1.555556 0 Td 52.932099 Tz (higher ) Tj 2.138889 0 Td 49.200337 Tz (radiopacity ) Tj 3.519444 0 Td 58.333333 Tz (and ) Tj 1.322222 0 Td 48.971193 Tz (indicated ) Tj 2.897222 0 Td 53.240741 Tz (implant ) Tj 2.469444 0 Td 45.619658 Tz (localization. ) Tj 51.428571 0 0 51.428571 0 835.43038 Tm 6.358333 0 Td 54.768519 Tz (Morselized ) Tj 3.558333 0 Td 49.583333 Tz (trabeculae ) Tj 3.208333 0 Td 57.253086 Tz (can ) Tj 1.283333 0 Td 59.953704 Tz (be ) Tj 0.991667 0 Td 52.662037 Tz (seen ) Tj 1.516667 0 Td 51.041667 Tz (adjacent ) Tj 2.702778 0 Td 45.37037 Tz (to ) Tj 0.855556 0 Td 48.611111 Tz (the ) Tj 1.147222 0 Td 52.777778 Tz (implant ) Tj 51.428571 0 0 51.428571 0 794.368932 Tm 6.377778 0 Td 48.251029 Tz (\(arrows\), ) Tj 2.780556 0 Td 48.611111 Tz (indicating ) Tj 3.111111 0 Td 51.851852 Tz (cohesiveness ) Tj 3.947222 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 49.691358 Tz (the ) Tj 1.088889 0 Td 50.771605 Tz (curing ) Tj 2.061111 0 Td 48.611111 Tz (material ) Tj 2.488889 0 Td 51.851852 Tz (as ) Tj 0.816667 0 Td 34.027778 Tz (it ) Tj 51.428571 0 0 51.428571 0 754.858182 Tm 6.358333 0 Td 38.888889 Tz (is ) Tj 0.661111 0 Td 54.444444 Tz (being ) Tj 1.827778 0 Td 47.314815 Tz (delivered. ) Tj 3.052778 0 Td 40.509259 Tz (a. ) Tj 0.738889 0 Td 53.742284 Tz (Radiographic ) Tj 4.083333 0 Td 59.62963 Tz (image ) Tj 1.963889 0 Td 58.333333 Tz (showing ) Tj 2.625 0 Td 52.256944 Tz (anterior ) Tj 51.428571 0 0 51.428571 0 714.228944 Tm 6.358333 0 Td 48.611111 Tz (posterior ) Tj 2.761111 0 Td 46.180556 Tz (section. ) Tj 2.391667 0 Td 42.12963 Tz (b. ) Tj 0.758333 0 Td 53.742284 Tz (Radiographic ) Tj 4.063889 0 Td 58.981481 Tz (image ) Tj 1.944444 0 Td 58.333333 Tz (showing ) Tj 2.605556 0 Td 49.259259 Tz (transverse ) Tj 51.428571 0 0 51.428571 0 669.485237 Tm 6.377778 0 Td 45.775463 Tz (section. ) Tj 2.547222 0 Td 38.888889 Tz (c. ) Tj 0.855556 0 Td 57.118056 Tz (Rendered ) Tj 3.052778 0 Td 59.62963 Tz (image ) Tj 2.080556 0 Td 58.333333 Tz (of ) Tj 0.933333 0 Td 54.012346 Tz (implanted ) Tj 3.266667 0 Td 46.090535 Tz (material. ) Tj 2.858333 0 Td 61.574074 Tz (22.8% ) Tj 51.428571 0 0 51.428571 0 628.825316 Tm 6.377778 0 Td 46.018519 Tz (\(V/v\) ) Tj 1.730556 0 Td 62.654321 Tz (was ) Tj 1.497222 0 Td 48.935185 Tz (calculated ) Tj 3.266667 0 Td 48.611111 Tz (for ) Tj 1.225 0 Td 50.231481 Tz (B-tricalcium ) Tj 3.966667 0 Td 55.092593 Tz (phosphate ) Tj 3.305556 0 Td 51.041667 Tz (granules ) Tj 51.428571 0 0 51.428571 0 585.456335 Tm 6.358333 0 Td 59.953704 Tz (compared ) Tj 3.091667 0 Td 43.75 Tz (to ) Tj 0.758333 0 Td 48.611111 Tz (the ) Tj 1.069444 0 Td 51.262626 Tz (formulation ) Tj 3.616667 0 Td 46.254209 Tz (theoretical ) Tj 3.286111 0 Td 51.203704 Tz (value ) Tj 1.730556 0 Td 58.333333 Tz (of ) Tj 0.797222 0 Td 72.376543 Tz (20% ) Tj 1.536111 0 Td 43.209877 Tz (\(v/v\), ) Tj 51.428571 0 0 51.428571 0 545.696682 Tm 6.358333 0 Td 49.691358 Tz (indicated ) Tj 2.8 0 Td 56.018519 Tz (homogenization ) Tj 4.9 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 48.611111 Tz (the ) Tj 1.088889 0 Td 58.981481 Tz (mixed ) Tj 1.944444 0 Td 49.421296 Tz (material ) Tj 2.547222 0 Td 38.888889 Tz (is ) Tj 0.602778 0 Td 53.292181 Tz (preserved ) Tj 51.428571 0 0 51.428571 0 509 Tm 6.358333 0 Td 52.932099 Tz (during ) Tj 2.080556 0 Td 45.046296 Tz (injection. ) Tj 51.428571 0 0 51.428571 0 459 Tm 7.116667 0 Td 54.282407 Tz (FIG. ) Tj 1.497222 0 Td 58.333333 Tz (12 ) Tj 0.875 0 Td 58.981481 Tz (shows ) Tj 1.963889 0 Td 47.314815 Tz (linearized ) Tj 3.013889 0 Td 77.777778 Tz (AC ) Tj 1.419444 0 Td 55.497685 Tz (measures ) Tj 2.838889 0 Td 47.530864 Tz (for ) Tj 0.972222 0 Td 45.802469 Tz (iducible-nitric ) Tj 51.428571 0 0 51.428571 0 420.05102 Tm 6.358333 0 Td 54.444444 Tz (oxide ) Tj 1.847222 0 Td 52.662037 Tz (synthase ) Tj 2.761111 0 Td 58.333333 Tz (\(iNOS\) ) Tj 2.372222 0 Td 52.662037 Tz (with ) Tj 1.536111 0 Td 68.055556 Tz (Rn ) Tj 0.894444 0 Td 50.771605 Tz (18s ) Tj 1.147222 0 Td 50.462963 Tz (histone ) Tj 2.352778 0 Td 57.523148 Tz (gene ) Tj 1.594444 0 Td 51.851852 Tz (taken ) Tj 1.788889 0 Td 50.231481 Tz (as ) Tj 0.855556 0 Td 55.092593 Tz (a ) Tj 51.428571 0 0 51.428571 0 380.657143 Tm 6.338889 0 Td 55.902778 Tz (housekeeping ) Tj 4.161111 0 Td 58.333333 Tz (gene ) Tj 1.555556 0 Td 48.148148 Tz (plotted ) Tj 2.216667 0 Td 49.074074 Tz (against ) Tj 2.216667 0 Td 58.333333 Tz (coded ) Tj 1.905556 0 Td 51.851852 Tz (treatment ) Tj 2.975 0 Td 49.691358 Tz (values ) Tj 1.963889 0 Td 40.509259 Tz (is ) Tj 51.428571 0 0 51.428571 0 335 Tm 6.377778 0 Td 61.574074 Tz (shown ) Tj 2.352778 0 Td 57.523148 Tz (\(F1+=-1; ) Tj 3.286111 0 Td 56.712963 Tz (F2+=-0.98: ) Tj 3.947222 0 Td 51.851852 Tz (Basal ) Tj 2.061111 0 Td 51.203704 Tz (\(time ) Tj 2.041667 0 Td 51.851852 Tz (zero\)=-0.94: ) Tj 51.428571 0 0 51.428571 0 293.745205 Tm 6.377778 0 Td 56.388889 Tz (F2-=-0.57; ) Tj 3.791667 0 Td 56.944444 Tz (LPS=1\). ) Tj 2.722222 0 Td 63.518519 Tz (Coded ) Tj 2.255556 0 Td 49.691358 Tz (values ) Tj 2.119444 0 Td 55.092593 Tz (span ) Tj 1.672222 0 Td 56.712963 Tz (-1 ) Tj 1.088889 0 Td 43.75 Tz (to ) Tj 0.933333 0 Td 32.407407 Tz (1 ) Tj 0.583333 0 Td 60.493827 Tz (and ) Tj 1.380556 0 Td 50.771605 Tz (are ) Tj 51.428571 0 0 51.428571 0 255.52218 Tm 6.358333 0 Td 49.305556 Tz (proportionally ) Tj 4.491667 0 Td 54.552469 Tz (spaced ) Tj 2.236111 0 Td 55.740741 Tz (based ) Tj 1.983333 0 Td 58.333333 Tz (on ) Tj 1.011111 0 Td 50.771605 Tz (the ) Tj 1.205556 0 Td 64.814815 Tz (mean ) Tj 1.866667 0 Td 53.472222 Tz (response ) Tj 2.838889 0 Td 50.771605 Tz (differ ) Tj 51.428571 0 0 51.428571 0 212.36 Tm 6.358333 0 Td 49.151235 Tz (ences. ) Tj 1.983333 0 Td 84.259259 Tz (A ) Tj 0.758333 0 Td 46.450617 Tz (linear ) Tj 1.866667 0 Td 57.361111 Tz (dependence ) Tj 3.655556 0 Td 58.333333 Tz (of ) Tj 0.855556 0 Td 49.016204 Tz (material ) Tj 2.586111 0 Td 38.888889 Tz (is ) Tj 0.7 0 Td 50.925926 Tz (evident ) Tj 2.352778 0 Td 45.37037 Tz (after ) Tj 1.555556 0 Td 63.734568 Tz (per ) Tj 51.428571 0 0 51.428571 0 170.818859 Tm 6.358333 0 Td 56.018519 Tz (forming ) Tj 2.605556 0 Td 43.75 Tz (this ) Tj 1.361111 0 Td 46.450617 Tz (linear ) Tj 1.905556 0 Td 50.925926 Tz (transformation ) Tj 4.569444 0 Td 56.712963 Tz (of ) Tj 0.875 0 Td 50.771605 Tz (the ) Tj 1.166667 0 Td 48.021886 Tz (categorical ) Tj 3.422222 0 Td 51.851852 Tz (treat ) Tj 30 0 0 30 0 2547 Tm 41.666667 0 Td 75 Tz (10 ) Tj 30 0 0 30 0 2339 Tm 41.666667 0 Td 72.222222 Tz (15 ) Tj 30 0 0 30 0 1923 Tm 41.6 0 Td 77.777778 Tz (25 ) Tj 30 0 0 30 0 1716 Tm 41.6 0 Td 80.555556 Tz (30 ) Tj 30 0 0 30 0 1507 Tm 41.6 0 Td 77.777778 Tz (35 ) Tj 30 0 0 30 0 1299 Tm 41.566667 0 Td 83.333333 Tz (40 ) Tj 30 0 0 30 0 1091 Tm 41.566667 0 Td 80.555556 Tz (45 ) Tj 30 0 0 30 0 883 Tm 41.6 0 Td 80.555556 Tz (50 ) Tj 30 0 0 30 0 675 Tm 41.6 0 Td 77.777778 Tz (55 ) Tj 30 0 0 30 0 467 Tm 41.633333 0 Td 77.777778 Tz (60 ) Tj 30 0 0 30 0 259 Tm 41.633333 0 Td 75 Tz (65 ) Tj 52.857143 0 0 52.857143 0 2968 Tm 32.9 0 Td 69.369369 Tz (6 ) Tj 51.428571 0 0 51.428571 0 2914.335045 Tm 25.297222 0 Td 60.763889 Tz (ment ) Tj 1.691667 0 Td 50.231481 Tz (data ) Tj 1.4 0 Td 60.493827 Tz (and ) Tj 1.302778 0 Td 61.574074 Tz (was ) Tj 1.341667 0 Td 52.256944 Tz (included ) Tj 2.741667 0 Td 48.611111 Tz (as ) Tj 0.836111 0 Td 55.092593 Tz (Such ) Tj 1.555556 0 Td 43.75 Tz (in ) Tj 0.797222 0 Td 49.691358 Tz (the ) Tj 1.108333 0 Td 53.472222 Tz (formal ) Tj 2.138889 0 Td 46.8107 Tz (analysis. ) Tj 51.428571 0 0 51.428571 0 2873.643282 Tm 25.297222 0 Td 52.932099 Tz (Upregulation ) Tj 3.966667 0 Td 53.472222 Tz (of ) Tj 0.719444 0 Td 68.055556 Tz (iNOS ) Tj 1.808333 0 Td 46.616809 Tz (transcription ) Tj 3.791667 0 Td 38.888889 Tz (is ) Tj 0.583333 0 Td 50.925926 Tz (evident ) Tj 2.275 0 Td 48.611111 Tz (for ) Tj 0.952778 0 Td 50.771605 Tz (the ) Tj 1.05 0 Td 63.734568 Tz (F2- ) Tj 1.244444 0 Td 60.493827 Tz (and ) Tj 50 0 0 50 0 2828.444444 Tm 26.04 0 Td 70 Tz (LPS ) Tj 1.48 0 Td 52.592593 Tz (treatment ) Tj 2.98 0 Td 52.380952 Tz (groups. ) Tj 51.428571 0 0 51.428571 0 2784 Tm 26.036111 0 Td 55.092593 Tz (FIG. ) Tj 1.672222 0 Td 53.472222 Tz (13 ) Tj 0.952778 0 Td 59.62963 Tz (shows ) Tj 2.080556 0 Td 47.962963 Tz (linearized ) Tj 3.15 0 Td 81.018519 Tz (AC ) Tj 1.536111 0 Td 55.902778 Tz (measures ) Tj 2.994444 0 Td 47.530864 Tz (for ) Tj 1.127778 0 Td 67.24537 Tz (iNOS ) Tj 1.925 0 Td 53.472222 Tz (with ) Tj 51.428571 0 0 51.428571 0 2748.928741 Tm 25.316667 0 Td 69.675926 Tz (Rn ) Tj 0.913889 0 Td 50.771605 Tz (18s ) Tj 1.05 0 Td 51.851852 Tz (taken ) Tj 1.711111 0 Td 48.611111 Tz (as ) Tj 0.719444 0 Td 58.333333 Tz (a ) Tj 0.447222 0 Td 55.902778 Tz (housekeeping ) Tj 4.141667 0 Td 56.712963 Tz (gene ) Tj 1.477778 0 Td 49.074074 Tz (plotted ) Tj 2.177778 0 Td 49.074074 Tz (against ) Tj 2.197222 0 Td 54.6875 Tz (exposure ) Tj 51.428571 0 0 51.428571 0 2702.478261 Tm 25.277778 0 Td 55.092593 Tz (time ) Tj 1.458333 0 Td 37.268519 Tz (is ) Tj 0.583333 0 Td 56.712963 Tz (shown. ) Tj 2.177778 0 Td 58.333333 Tz (Given ) Tj 1.886111 0 Td 50.771605 Tz (the ) Tj 1.030556 0 Td 53.240741 Tz (average ) Tj 2.391667 0 Td 43.75 Tz (relative ) Tj 2.255556 0 Td 49.421296 Tz (increase ) Tj 2.508333 0 Td 45.37037 Tz (in ) Tj 0.680556 0 Td 68.055556 Tz (iNOS ) Tj 1.769444 0 Td 61.574074 Tz (by ) Tj 51.428571 0 0 51.428571 0 2662.656679 Tm 25.277778 0 Td 50.771605 Tz (the ) Tj 1.127778 0 Td 56.712963 Tz (24 ) Tj 0.875 0 Td 57.523148 Tz (hour ) Tj 1.555556 0 Td 54.282407 Tz (time ) Tj 1.458333 0 Td 51.203704 Tz (point ) Tj 1.711111 0 Td 53.877315 Tz (followed ) Tj 2.741667 0 Td 61.574074 Tz (by ) Tj 0.952778 0 Td 65.625 Tz (down ) Tj 1.827778 0 Td 48.287037 Tz (regulation ) Tj 3.072222 0 Td 59.143519 Tz (back ) Tj 1.594444 0 Td 45.37037 Tz (to ) Tj 51.428571 0 0 51.428571 0 2621.228792 Tm 25.297222 0 Td 45.910494 Tz (levels ) Tj 1.925 0 Td 56.712963 Tz (comparable ) Tj 3.694444 0 Td 43.75 Tz (to ) Tj 0.816667 0 Td 46.180556 Tz (that ) Tj 1.341667 0 Td 59.953704 Tz (of ) Tj 0.875 0 Td 49.907407 Tz (basal ) Tj 1.769444 0 Td 51.851852 Tz (\(time ) Tj 1.827778 0 Td 45.910494 Tz (zero\), ) Tj 1.925 0 Td 51.851852 Tz (a ) Tj 0.583333 0 Td 50.05144 Tz (parabolic ) Tj 51.428571 0 0 51.428571 0 2577.316583 Tm 25.297222 0 Td 50.231481 Tz (function ) Tj 2.663889 0 Td 60.493827 Tz (was ) Tj 1.322222 0 Td 55.632716 Tz (chosen ) Tj 2.197222 0 Td 48.611111 Tz (to ) Tj 0.797222 0 Td 50.636574 Tz (describe ) Tj 2.625 0 Td 43.75 Tz (this ) Tj 1.283333 0 Td 53.06713 Tz (response ) Tj 2.780556 0 Td 43.75 Tz (in ) Tj 0.738889 0 Td 50.771605 Tz (the ) Tj 1.127778 0 Td 53.472222 Tz (formal ) Tj 51.428571 0 0 51.428571 0 2543 Tm 25.297222 0 Td 46.450617 Tz (analysis. ) Tj 38.571429 0 0 38.571429 0 2464 Tm 35.181481 0 Td 97.762346 Tz (DETAILED ) Tj 4.977778 0 Td 94.276094 Tz (DESCRIPTION ) Tj 6.533333 0 Td 99.382716 Tz (OF ) Tj 1.4 0 Td 106.584362 Tz (THE ) Tj 2.177778 0 Td 98.902606 Tz (INVENTION ) Tj 51.428571 0 0 51.428571 0 2378.405405 Tm 26.016667 0 Td 75.617284 Tz (Now ) Tj 1.575 0 Td 46.8107 Tz (referring ) Tj 2.683333 0 Td 45.37037 Tz (to ) Tj 0.758333 0 Td 53.472222 Tz (FIG. ) Tj 1.497222 0 Td 42.12963 Tz (1, ) Tj 0.680556 0 Td 55.092593 Tz (an ) Tj 0.836111 0 Td 45.664983 Tz (injectable, ) Tj 3.169444 0 Td 45.37037 Tz (in ) Tj 0.719444 0 Td 46.180556 Tz (situ-curing, ) Tj 3.480556 0 Td 55.902778 Tz (algi ) Tj 51.428571 0 0 51.428571 0 2335.344242 Tm 25.297222 0 Td 51.851852 Tz (nate-based ) Tj 3.325 0 Td 53.877315 Tz (hydrogel ) Tj 2.819444 0 Td 38.888889 Tz (is ) Tj 0.738889 0 Td 55.632716 Tz (shown. ) Tj 2.236111 0 Td 58.333333 Tz (These ) Tj 1.983333 0 Td 50 Tz (alginate-based ) Tj 4.433333 0 Td 64.166667 Tz (hydro ) Tj 51.428571 0 0 51.428571 0 2293.394541 Tm 25.297222 0 Td 48.611111 Tz (gels ) Tj 1.361111 0 Td 55.092593 Tz (can ) Tj 1.166667 0 Td 58.333333 Tz (be ) Tj 0.875 0 Td 55.902778 Tz (used ) Tj 1.516667 0 Td 45.37037 Tz (in ) Tj 0.719444 0 Td 58.333333 Tz (a ) Tj 0.525 0 Td 51.851852 Tz (process ) Tj 2.372222 0 Td 47.530864 Tz (for ) Tj 1.030556 0 Td 55.092593 Tz (remodeling ) Tj 3.480556 0 Td 51.446759 Tz (diseased ) Tj 2.644444 0 Td 58.981481 Tz (osteo ) Tj 51.428571 0 0 51.428571 0 2250.643478 Tm 25.297222 0 Td 52.662037 Tz (chondral ) Tj 2.975 0 Td 59.953704 Tz (bone ) Tj 1.905556 0 Td 45.37037 Tz (to ) Tj 1.030556 0 Td 45.37037 Tz (that ) Tj 1.516667 0 Td 58.333333 Tz (of ) Tj 1.05 0 Td 55.092593 Tz (a ) Tj 0.777778 0 Td 51.388889 Tz (healthy ) Tj 2.644444 0 Td 40.509259 Tz (state, ) Tj 1.944444 0 Td 54.166667 Tz (evading ) Tj 2.722222 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 2211.574194 Tm 25.297222 0 Td 51.262626 Tz (progression ) Tj 3.694444 0 Td 55.092593 Tz (of ) Tj 0.816667 0 Td 46.666667 Tz (joint ) Tj 1.652778 0 Td 51.851852 Tz (degeneration ) Tj 4.005556 0 Td 46.990741 Tz (in ) Tj 0.875 0 Td 57.253086 Tz (one ) Tj 1.302778 0 Td 47.222222 Tz (aspect, ) Tj 2.275 0 Td 51.851852 Tz (or ) Tj 0.894444 0 Td 54.012346 Tz (can ) Tj 1.244444 0 Td 59.953704 Tz (be ) Tj 51.428571 0 0 51.428571 0 2168.13422 Tm 25.297222 0 Td 50.925926 Tz (applied ) Tj 2.255556 0 Td 48.611111 Tz (to ) Tj 0.738889 0 Td 55.092593 Tz (an ) Tj 0.816667 0 Td 49.537037 Tz (injured ) Tj 2.216667 0 Td 51.851852 Tz (or ) Tj 0.738889 0 Td 51.446759 Tz (diseased ) Tj 2.605556 0 Td 53.703704 Tz (osseous ) Tj 2.411111 0 Td 42.12963 Tz (site ) Tj 1.166667 0 Td 50.771605 Tz (within ) Tj 1.963889 0 Td 58.333333 Tz (a ) Tj 0.486111 0 Td 49.537037 Tz (subject ) Tj 51.428571 0 0 51.428571 0 2126.395683 Tm 25.277778 0 Td 46.990741 Tz (to ) Tj 0.758333 0 Td 42.453704 Tz (facilitate ) Tj 2.722222 0 Td 47.800926 Tz (healing. ) Tj 51.428571 0 0 51.428571 0 2084.57754 Tm 26.016667 0 Td 53.472222 Tz (This ) Tj 1.516667 0 Td 50.378788 Tz (biomaterial ) Tj 3.597222 0 Td 55.452675 Tz (composite ) Tj 3.227778 0 Td 54.012346 Tz (can ) Tj 1.244444 0 Td 58.333333 Tz (be ) Tj 0.952778 0 Td 55.092593 Tz (used ) Tj 1.555556 0 Td 45.37037 Tz (to ) Tj 0.836111 0 Td 46.990741 Tz (repair ) Tj 1.905556 0 Td 59.41358 Tz (and ) Tj 1.302778 0 Td 53.472222 Tz (or ) Tj 51.428571 0 0 51.428571 0 2041.561952 Tm 25.297222 0 Td 50.555556 Tz (regenerate ) Tj 3.247222 0 Td 59.143519 Tz (bone ) Tj 1.633333 0 Td 59.41358 Tz (and ) Tj 1.302778 0 Td 37.808642 Tz (its ) Tj 0.913889 0 Td 50.231481 Tz (associated ) Tj 3.227778 0 Td 43.75 Tz (tissues, ) Tj 2.372222 0 Td 54.282407 Tz (such ) Tj 1.555556 0 Td 48.611111 Tz (as ) Tj 0.855556 0 Td 55.555556 Tz (marrow, ) Tj 51.428571 0 0 51.428571 0 2002.96478 Tm 25.297222 0 Td 50.084175 Tz (vasculature ) Tj 3.577778 0 Td 59.41358 Tz (and ) Tj 1.361111 0 Td 53.472222 Tz (or ) Tj 0.913889 0 Td 50.636574 Tz (adjacent ) Tj 2.722222 0 Td 44.722222 Tz (cartilage, ) Tj 2.994444 0 Td 53.06713 Tz (ligament ) Tj 2.819444 0 Td 53.472222 Tz (or ) Tj 0.913889 0 Td 50.925926 Tz (tendon, ) Tj 51.428571 0 0 51.428571 0 1957.871481 Tm 25.316667 0 Td 53.324916 Tz (Subchondral ) Tj 3.713889 0 Td 53.472222 Tz (or ) Tj 0.777778 0 Td 50.87963 Tz (cancellous ) Tj 3.227778 0 Td 52.5 Tz (bone, ) Tj 1.769444 0 Td 58.333333 Tz (and ) Tj 1.205556 0 Td 48.070988 Tz (spinal ) Tj 1.925 0 Td 47.800926 Tz (disc ) Tj 1.341667 0 Td 50.231481 Tz (or ) Tj 0.758333 0 Td 45.37037 Tz (to ) Tj 0.738889 0 Td 48.148148 Tz (deliver ) Tj 51.428571 0 0 51.428571 0 1917.776699 Tm 25.297222 0 Td 49.907407 Tz (other ) Tj 1.691667 0 Td 49.691358 Tz (bioactive ) Tj 2.858333 0 Td 52.391975 Tz (agents ) Tj 2.119444 0 Td 48.611111 Tz (for ) Tj 1.030556 0 Td 51.851852 Tz (other ) Tj 1.769444 0 Td 54.166667 Tz (medical ) Tj 2.488889 0 Td 47.115385 Tz (applications. ) Tj 3.947222 0 Td 65.625 Tz (More ) Tj 51.428571 0 0 51.428571 0 1878.333736 Tm 25.316667 0 Td 43.874644 Tz (specifically, ) Tj 3.577778 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 50.378788 Tz (biomaterial ) Tj 3.519444 0 Td 55.092593 Tz (composite ) Tj 3.15 0 Td 55.092593 Tz (can ) Tj 1.166667 0 Td 58.333333 Tz (be ) Tj 0.855556 0 Td 48.206019 Tz (injected ) Tj 2.508333 0 Td 46.180556 Tz (into ) Tj 1.283333 0 Td 55.092593 Tz (a ) Tj 51.428571 0 0 51.428571 0 1835.867008 Tm 25.316667 0 Td 41.319444 Tz (site ) Tj 1.186111 0 Td 46.990741 Tz (in ) Tj 0.777778 0 Td 55.092593 Tz (a ) Tj 0.525 0 Td 46.180556 Tz (Subject. ) Tj 2.411111 0 Td 54.282407 Tz (This ) Tj 1.536111 0 Td 41.319444 Tz (site ) Tj 1.205556 0 Td 54.012346 Tz (can ) Tj 1.186111 0 Td 58.333333 Tz (be ) Tj 0.875 0 Td 59.953704 Tz (bone ) Tj 1.633333 0 Td 51.446759 Tz (adjacent ) Tj 2.625 0 Td 46.990741 Tz (to ) Tj 0.797222 0 Td 44.398148 Tz (cartilage; ) Tj 51.428571 0 0 51.428571 0 1795.439898 Tm 25.316667 0 Td 54.282407 Tz (Such ) Tj 1.594444 0 Td 44.290123 Tz (as, ) Tj 1.108333 0 Td 46.450617 Tz (for ) Tj 1.127778 0 Td 54.6875 Tz (example, ) Tj 2.897222 0 Td 45.730453 Tz (articular ) Tj 2.761111 0 Td 46.090535 Tz (cartilage ) Tj 2.761111 0 Td 58.333333 Tz (of ) Tj 0.913889 0 Td 55.092593 Tz (an ) Tj 0.952778 0 Td 46.180556 Tz (articulating ) Tj 51.428571 0 0 51.428571 0 1751.594595 Tm 25.258333 0 Td 43.209877 Tz (joint, ) Tj 1.730556 0 Td 45.37037 Tz (in ) Tj 0.719444 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 58.333333 Tz (knee ) Tj 1.555556 0 Td 58.333333 Tz (and ) Tj 1.244444 0 Td 44.560185 Tz (hip. ) Tj 1.244444 0 Td 64.814815 Tz (The ) Tj 1.341667 0 Td 46.450617 Tz (cartilage ) Tj 2.683333 0 Td 55.092593 Tz (can ) Tj 1.166667 0 Td 51.388889 Tz (include ) Tj 2.313889 0 Td 45.730453 Tz (articular ) Tj 51.428571 0 0 51.428571 0 1712.778808 Tm 25.297222 0 Td 58.333333 Tz (and ) Tj 1.380556 0 Td 44.722222 Tz (fibrocartilage. ) Tj 4.355556 0 Td 35.648148 Tz (It ) Tj 0.738889 0 Td 55.092593 Tz (can ) Tj 1.283333 0 Td 59.953704 Tz (be ) Tj 1.030556 0 Td 47.800926 Tz (injected ) Tj 2.605556 0 Td 46.180556 Tz (into ) Tj 1.4 0 Td 59.41358 Tz (and ) Tj 1.361111 0 Td 51.041667 Tz (adjacent ) Tj 2.741667 0 Td 45.37037 Tz (to ) Tj 51.428571 0 0 51.428571 0 1669.012107 Tm 25.297222 0 Td 50.771605 Tz (endplates ) Tj 2.936111 0 Td 58.333333 Tz (of ) Tj 0.816667 0 Td 47.170782 Tz (vertebral ) Tj 2.761111 0 Td 43.425926 Tz (disc, ) Tj 1.516667 0 Td 53.472222 Tz (with ) Tj 1.497222 0 Td 48.611111 Tz (the ) Tj 1.05 0 Td 47.890947 Tz (intention ) Tj 2.780556 0 Td 59.953704 Tz (of ) Tj 0.816667 0 Td 55.092593 Tz (remodeling ) Tj 52.857143 0 0 52.857143 0 1625.731946 Tm 24.594595 0 Td 49.399399 Tz (the ) Tj 1.116216 0 Td 51.081081 Tz (underlying ) Tj 3.291892 0 Td 52.457002 Tz (subchondral ) Tj 3.689189 0 Td 58.333333 Tz (bone ) Tj 1.589189 0 Td 45.720721 Tz (to ) Tj 0.813514 0 Td 53.603604 Tz (a ) Tj 0.52973 0 Td 46.114865 Tz (healthy, ) Tj 2.440541 0 Td 49.399399 Tz (vascularized ) Tj 51.428571 0 0 51.428571 0 1583.863415 Tm 25.316667 0 Td 41.049383 Tz (state. ) Tj 1.633333 0 Td 62.654321 Tz (The ) Tj 1.302778 0 Td 50.084175 Tz (biomaterial ) Tj 3.480556 0 Td 55.452675 Tz (composite ) Tj 3.15 0 Td 55.092593 Tz (can ) Tj 1.147222 0 Td 59.953704 Tz (be ) Tj 0.875 0 Td 49.331276 Tz (delivered ) Tj 2.838889 0 Td 43.75 Tz (to ) Tj 0.719444 0 Td 59.143519 Tz (bone ) Tj 1.575 0 Td 59.953704 Tz (by ) Tj 51.428571 0 0 51.428571 0 1542.498065 Tm 25.297222 0 Td 44.965278 Tz (drilling ) Tj 2.333333 0 Td 53.472222 Tz (hole ) Tj 1.497222 0 Td 52.662037 Tz (with ) Tj 1.477778 0 Td 51.851852 Tz (a ) Tj 0.525 0 Td 39.537037 Tz (drill ) Tj 1.4 0 Td 44.290123 Tz (bit ) Tj 0.972222 0 Td 55.092593 Tz (or ) Tj 0.836111 0 Td 50.555556 Tz (other ) Tj 1.691667 0 Td 46.990741 Tz (tool ) Tj 1.361111 0 Td 53.472222 Tz (such ) Tj 1.497222 0 Td 51.851852 Tz (as ) Tj 0.836111 0 Td 51.851852 Tz (a ) Tj 0.486111 0 Td 53.06713 Tz (jamshidi ) Tj 51.428571 0 0 51.428571 0 1500.352113 Tm 25.316667 0 Td 55.092593 Tz (\(bone ) Tj 1.788889 0 Td 50 Tz (needle\) ) Tj 2.236111 0 Td 51.851852 Tz (accessing ) Tj 2.916667 0 Td 50.771605 Tz (the ) Tj 1.05 0 Td 53.324916 Tz (Subchondral ) Tj 3.655556 0 Td 59.41358 Tz (and ) Tj 1.186111 0 Td 51.203704 Tz (cancellous ) Tj 3.169444 0 Td 59.953704 Tz (bone ) Tj 51.428571 0 0 51.428571 0 1460.424426 Tm 25.297222 0 Td 50.636574 Tz (adjacent ) Tj 2.586111 0 Td 48.611111 Tz (to ) Tj 0.777778 0 Td 56.388889 Tz (upper ) Tj 1.827778 0 Td 59.41358 Tz (and ) Tj 1.244444 0 Td 55.740741 Tz (lower ) Tj 1.847222 0 Td 47.170782 Tz (vertebral ) Tj 2.722222 0 Td 51.131687 Tz (endplates ) Tj 2.955556 0 Td 58.333333 Tz (and ) Tj 1.225 0 Td 54.166667 Tz (annulus ) Tj 51.428571 0 0 51.428571 0 1418.861386 Tm 25.297222 0 Td 47.800926 Tz (fibrosus ) Tj 2.508333 0 Td 58.333333 Tz (of ) Tj 0.797222 0 Td 58.333333 Tz (a ) Tj 0.563889 0 Td 48.070988 Tz (spinal ) Tj 1.963889 0 Td 47.800926 Tz (disc ) Tj 1.380556 0 Td 51.851852 Tz (or ) Tj 0.797222 0 Td 45.730453 Tz (articular ) Tj 2.644444 0 Td 46.450617 Tz (cartilage ) Tj 2.722222 0 Td 45.37037 Tz (in ) Tj 0.758333 0 Td 46.450617 Tz (articulating ) Tj 51.428571 0 0 51.428571 0 1377.323944 Tm 25.258333 0 Td 45.910494 Tz (joints ) Tj 1.75 0 Td 60.493827 Tz (and ) Tj 1.205556 0 Td 49.691358 Tz (the ) Tj 0.991667 0 Td 50.378788 Tz (biomaterial ) Tj 3.461111 0 Td 55.092593 Tz (composite ) Tj 3.111111 0 Td 55.092593 Tz (can ) Tj 1.108333 0 Td 58.333333 Tz (be ) Tj 0.816667 0 Td 49.691358 Tz (delivered ) Tj 2.8 0 Td 53.703704 Tz (through ) Tj 51.428571 0 0 51.428571 0 1334.706173 Tm 25.277778 0 Td 43.75 Tz (this ) Tj 1.244444 0 Td 53.472222 Tz (hole ) Tj 1.477778 0 Td 51.851852 Tz (or ) Tj 0.797222 0 Td 55.555556 Tz (opening ) Tj 2.547222 0 Td 43.75 Tz (in ) Tj 0.719444 0 Td 50.771605 Tz (the ) Tj 1.108333 0 Td 51.851852 Tz (bone. ) Tj 1.75 0 Td 63.734568 Tz (The ) Tj 1.322222 0 Td 50.378788 Tz (biomaterial ) Tj 3.538889 0 Td 55.452675 Tz (composite ) Tj 51.428571 0 0 51.428571 0 1294.838752 Tm 25.297222 0 Td 55.092593 Tz (can ) Tj 1.263889 0 Td 58.333333 Tz (be ) Tj 0.991667 0 Td 49.691358 Tz (delivered ) Tj 2.936111 0 Td 51.041667 Tz (adjacent ) Tj 2.722222 0 Td 45.37037 Tz (to ) Tj 0.855556 0 Td 51.851852 Tz (or ) Tj 0.875 0 Td 46.180556 Tz (into ) Tj 1.419444 0 Td 44.074074 Tz (cartilage. ) Tj 2.936111 0 Td 62.654321 Tz (The ) Tj 1.4 0 Td 49.537037 Tz (Subject ) Tj 51.428571 0 0 51.428571 0 1250.227673 Tm 25.297222 0 Td 50.231481 Tz (includes ) Tj 2.722222 0 Td 61.979167 Tz (mammals, ) Tj 3.247222 0 Td 55.092593 Tz (such ) Tj 1.575 0 Td 50.231481 Tz (as ) Tj 0.894444 0 Td 57.87037 Tz (humans, ) Tj 2.702778 0 Td 49.421296 Tz (equines, ) Tj 2.663889 0 Td 48.611111 Tz (canines, ) Tj 2.625 0 Td 42.12963 Tz (etc. ) Tj 51.428571 0 0 51.428571 0 1211.742857 Tm 25.297222 0 Td 63.734568 Tz (The ) Tj 1.283333 0 Td 50.925926 Tz (present ) Tj 2.294444 0 Td 50.05144 Tz (invention ) Tj 2.877778 0 Td 48.611111 Tz (also ) Tj 1.341667 0 Td 52.256944 Tz (provides ) Tj 2.663889 0 Td 58.333333 Tz (a ) Tj 0.505556 0 Td 50.378788 Tz (biomaterial ) Tj 3.519444 0 Td 55.452675 Tz (composite ) Tj 51.428571 0 0 51.428571 0 1168.816901 Tm 25.297222 0 Td 49.691358 Tz (for ) Tj 1.030556 0 Td 49.691358 Tz (the ) Tj 1.069444 0 Td 49.259259 Tz (controlled ) Tj 3.15 0 Td 48.611111 Tz (delivery ) Tj 2.527778 0 Td 56.712963 Tz (of ) Tj 0.797222 0 Td 48.611111 Tz (bioactive ) Tj 2.780556 0 Td 48.611111 Tz (agents. ) Tj 51.428571 0 0 51.428571 0 1128.517879 Tm 26.016667 0 Td 63.734568 Tz (The ) Tj 1.283333 0 Td 55.812757 Tz (composite ) Tj 3.15 0 Td 48.251029 Tz (scaffolds ) Tj 2.780556 0 Td 49.691358 Tz (are ) Tj 1.030556 0 Td 50.05144 Tz (primarily ) Tj 2.897222 0 Td 64.814815 Tz (made ) Tj 1.691667 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 53.148148 Tz (analginate ) Tj 51.428571 0 0 51.428571 0 1085.864198 Tm 25.277778 0 Td 53.877315 Tz (hydrogel ) Tj 2.916667 0 Td 52.662037 Tz (with ) Tj 1.594444 0 Td 55.092593 Tz (dense ) Tj 1.963889 0 Td 56.532922 Tz (nonporous ) Tj 3.383333 0 Td 51.851852 Tz (micron-scale ) Tj 4.161111 0 Td 48.070988 Tz (B-tricalcium ) Tj 51.428571 0 0 51.428571 0 1044.906005 Tm 25.297222 0 Td 54.73251 Tz (phosphate ) Tj 3.266667 0 Td 49.789562 Tz (aggregates. ) Tj 3.655556 0 Td 50.231481 Tz (See, ) Tj 1.555556 0 Td 42.939815 Tz (e.g. ) Tj 1.4 0 Td 53.472222 Tz (U.S. ) Tj 1.672222 0 Td 42.939815 Tz (Pat. ) Tj 1.419444 0 Td 57.253086 Tz (No. ) Tj 1.4 0 Td 51.203704 Tz (7.754,246. ) Tj 51.428571 0 0 51.428571 0 1003.19585 Tm 25.316667 0 Td 60.277778 Tz (Dense ) Tj 2.411111 0 Td 55.812757 Tz (nonporous ) Tj 3.636111 0 Td 51.851852 Tz (micron-scale ) Tj 4.394444 0 Td 48.070988 Tz (B-tricalcium ) Tj 4.044444 0 Td 55.092593 Tz (phosphate ) Tj 51.428571 0 0 51.428571 0 963.767901 Tm 25.297222 0 Td 52.211934 Tz (aggregate ) Tj 3.052778 0 Td 37.268519 Tz (is ) Tj 0.680556 0 Td 55.092593 Tz (a ) Tj 0.525 0 Td 45.100309 Tz (particulate. ) Tj 3.441667 0 Td 64.814815 Tz (The ) Tj 1.380556 0 Td 52.211934 Tz (aggregate ) Tj 3.013889 0 Td 45.730453 Tz (particles ) Tj 2.702778 0 Td 50.462963 Tz (contain ) Tj 2.352778 0 Td 55.092593 Tz (a ) Tj 51.428571 0 0 51.428571 0 919.676923 Tm 25.297222 0 Td 57.407407 Tz (minimal ) Tj 2.741667 0 Td 61.574074 Tz (amount ) Tj 2.547222 0 Td 56.712963 Tz (of ) Tj 0.913889 0 Td 49.826389 Tz (porosity ) Tj 2.761111 0 Td 57.685185 Tz (which ) Tj 2.1 0 Td 38.888889 Tz (is ) Tj 0.797222 0 Td 49.691358 Tz (generally ) Tj 3.033333 0 Td 53.877315 Tz (achieved ) Tj 51.428571 0 0 51.428571 0 880.003745 Tm 25.277778 0 Td 54.166667 Tz (through ) Tj 2.586111 0 Td 51.851852 Tz (a ) Tj 0.641667 0 Td 46.8107 Tz (sintering ) Tj 2.819444 0 Td 51.388889 Tz (process ) Tj 2.469444 0 Td 46.342593 Tz (\(typically ) Tj 3.091667 0 Td 57.87037 Tz (between ) Tj 2.780556 0 Td 57.253086 Tz (1180-1450 ) Tj 51.428571 0 0 51.428571 0 838.298204 Tm 25.297222 0 Td 52.314815 Tz (degrees ) Tj 2.411111 0 Td 50.673401 Tz (Centigrade, ) Tj 3.538889 0 Td 49.907407 Tz (preferably ) Tj 3.188889 0 Td 46.990741 Tz (at ) Tj 0.816667 0 Td 57.253086 Tz (1300-1400 ) Tj 3.286111 0 Td 52.777778 Tz (degrees ) Tj 2.391667 0 Td 60.925926 Tz (Centi ) Tj 51.428571 0 0 51.428571 0 795.459716 Tm 25.297222 0 Td 51.311728 Tz (grade\) ) Tj 2.022222 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 51.851852 Tz (smaller ) Tj 2.333333 0 Td 46.018519 Tz (\(typically ) Tj 2.936111 0 Td 53.619529 Tz (Sub-micron\) ) Tj 3.713889 0 Td 50.555556 Tz (sized ) Tj 1.652778 0 Td 46.090535 Tz (particles ) Tj 2.663889 0 Td 58.333333 Tz (from ) Tj 51.428571 0 0 51.428571 0 751.945137 Tm 25.297222 0 Td 55.092593 Tz (a ) Tj 0.583333 0 Td 53.148148 Tz (green ) Tj 1.886111 0 Td 48.611111 Tz (\(unsintered\) ) Tj 3.752778 0 Td 45.775463 Tz (particle ) Tj 2.430556 0 Td 48.611111 Tz (to ) Tj 0.855556 0 Td 51.851852 Tz (a ) Tj 0.602778 0 Td 50.771605 Tz (consolidated ) Tj 3.927778 0 Td 51.851852 Tz (\(continuous ) Tj 51.428571 0 0 51.428571 0 712.489796 Tm 25.316667 0 Td 45.37037 Tz (solid\) ) Tj 1.944444 0 Td 43.209877 Tz (particle. ) Tj 2.683333 0 Td 55.740741 Tz (Other ) Tj 1.983333 0 Td 57.87037 Tz (methods ) Tj 2.741667 0 Td 58.333333 Tz (of ) Tj 0.913889 0 Td 52.5 Tz (production ) Tj 3.519444 0 Td 64.814815 Tz (may ) Tj 1.516667 0 Td 51.388889 Tz (include ) Tj 51.428571 0 0 51.428571 0 671.019324 Tm 25.297222 0 Td 54.73251 Tz (producing ) Tj 3.091667 0 Td 58.333333 Tz (a ) Tj 0.505556 0 Td 54.166667 Tz (calcium ) Tj 2.45 0 Td 54.73251 Tz (phosphate ) Tj 3.15 0 Td 52.662037 Tz (melt ) Tj 1.419444 0 Td 58.333333 Tz (which ) Tj 1.925 0 Td 60.925926 Tz (would ) Tj 2.002778 0 Td 45.37037 Tz (solidify ) Tj 2.352778 0 Td 45.37037 Tz (to ) Tj 51.428571 0 0 51.428571 0 626.646983 Tm 25.297222 0 Td 55.092593 Tz (a ) Tj 0.583333 0 Td 52.824074 Tz (continuous ) Tj 3.441667 0 Td 43.209877 Tz (solid. ) Tj 1.808333 0 Td 53.472222 Tz (This ) Tj 1.575 0 Td 46.018519 Tz (solid ) Tj 1.652778 0 Td 49.331276 Tz (construct ) Tj 2.897222 0 Td 53.796296 Tz (could ) Tj 1.847222 0 Td 51.851852 Tz (then ) Tj 1.516667 0 Td 56.712963 Tz (be ) Tj 0.913889 0 Td 68.055556 Tz (pro ) Tj 51.428571 0 0 51.428571 0 589.68059 Tm 25.297222 0 Td 52.932099 Tz (cessed ) Tj 2.138889 0 Td 45.37037 Tz (to ) Tj 0.797222 0 Td 53.240741 Tz (through ) Tj 2.508333 0 Td 46.990741 Tz (milling, ) Tj 2.488889 0 Td 48.971193 Tz (grinding, ) Tj 2.897222 0 Td 51.851852 Tz (or ) Tj 0.836111 0 Td 50.555556 Tz (other ) Tj 1.711111 0 Td 52.599715 Tz (fragmentation ) Tj 51.428571 0 0 51.428571 0 545.666667 Tm 25.277778 0 Td 51.851852 Tz (techniques ) Tj 3.286111 0 Td 53.148148 Tz (alone ) Tj 1.788889 0 Td 51.851852 Tz (or ) Tj 0.777778 0 Td 43.75 Tz (in ) Tj 0.738889 0 Td 54.503367 Tz (combination ) Tj 3.791667 0 Td 53.472222 Tz (with ) Tj 1.458333 0 Td 55.092593 Tz (a ) Tj 0.525 0 Td 49.074074 Tz (sieving ) Tj 2.236111 0 Td 53.472222 Tz (or ) Tj 0.777778 0 Td 58.333333 Tz (separa ) Tj 51.428571 0 0 51.428571 0 505.506509 Tm 25.277778 0 Td 46.990741 Tz (tion ) Tj 1.244444 0 Td 52.314815 Tz (process ) Tj 2.294444 0 Td 48.611111 Tz (to ) Tj 0.719444 0 Td 48.003472 Tz (generate/collect ) Tj 4.705556 0 Td 45.37037 Tz (particles ) Tj 2.605556 0 Td 51.851852 Tz (of ) Tj 0.7 0 Td 55.092593 Tz (a ) Tj 0.466667 0 Td 49.537037 Tz (desired ) Tj 2.197222 0 Td 42.777778 Tz (size. ) Tj 1.419444 0 Td 63.734568 Tz (The ) Tj 51.428571 0 0 51.428571 0 462.145959 Tm 25.316667 0 Td 46.666667 Tz (Solid ) Tj 1.555556 0 Td 49.331276 Tz (construct ) Tj 2.838889 0 Td 66.975309 Tz (may ) Tj 1.361111 0 Td 59.953704 Tz (be ) Tj 0.875 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 49.691358 Tz (the ) Tj 1.166667 0 Td 47.800926 Tz (B-tricalcium ) Tj 3.616667 0 Td 55.092593 Tz (phosphate ) Tj 3.130556 0 Td 51.388889 Tz (crystal ) Tj 51.428571 0 0 51.428571 0 421.436686 Tm 25.297222 0 Td 45.37037 Tz (line ) Tj 1.186111 0 Td 56.388889 Tz (phase ) Tj 1.788889 0 Td 53.472222 Tz (or ) Tj 0.738889 0 Td 49.691358 Tz (the ) Tj 0.972222 0 Td 53.652263 Tz (processed ) Tj 2.994444 0 Td 45.730453 Tz (particles ) Tj 2.586111 0 Td 54.444444 Tz (could ) Tj 1.730556 0 Td 59.953704 Tz (be ) Tj 0.855556 0 Td 44.444444 Tz (refired ) Tj 1.963889 0 Td 48.611111 Tz (to ) Tj 0.719444 0 Td 54.012346 Tz (induce ) Tj 51.428571 0 0 51.428571 0 379.36694 Tm 25.297222 0 Td 52.932099 Tz (formation ) Tj 3.013889 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 54.282407 Tz (such ) Tj 1.419444 0 Td 56.388889 Tz (phase ) Tj 1.808333 0 Td 51.851852 Tz (before ) Tj 2.002778 0 Td 51.851852 Tz (or ) Tj 0.7 0 Td 46.018519 Tz (after ) Tj 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42.592593 Tz (lattice ) Tj 1.963889 0 Td 54.444444 Tz (struc ) Tj 40 0 0 40 0 218 Tm 32.5 0 Td 55.833333 Tz (ture. ) Tj 2 0 Td 63.425926 Tz (Variation ) Tj 3.8 0 Td 73.958333 Tz (from ) Tj 2.175 0 Td 59.469697 Tz (theoretical ) Tj 4.275 0 Td 65.277778 Tz (values ) Tj 2.7 0 Td 52.083333 Tz (is ) Tj 0.975 0 Td 70.833333 Tz (a ) Tj 0.8 0 Td 57.638889 Tz (result ) Tj 2.425 0 Td 70.833333 Tz (of ) Tj 1.125 0 Td 75 Tz (minor ) Tj 51.428571 0 0 51.428571 0 170.809015 Tm 25.297222 0 Td 51.103989 Tz (imperfections ) Tj 4.141667 0 Td 47.395833 Tz (\(cracks, ) Tj 2.45 0 Td 58.981481 Tz (doped ) Tj 1.905556 0 Td 53.472222 Tz (elements ) Tj 2.722222 0 Td 52.5 Tz (\(such ) Tj 1.730556 0 Td 50.231481 Tz (as ) Tj 0.777778 0 Td 43.209877 Tz (silicon\), ) Tj 2.469444 0 Td 53.472222 Tz (and/ ) Tj ET Q endstream endobj 456 0 obj << /Length 1 >> stream q endstream endobj 457 0 obj << /Length 47601 >> stream Q q 0.24 0 0 0.24 0 0 cm BT /F1 1 Tf 3 Tr 62.857143 0 0 62.857143 0 3023 Tm 17.325 0 Td 83.522727 Tz 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2831.487242 Tm 6.358333 0 Td 46.990741 Tz (acts ) Tj 1.263889 0 Td 46.990741 Tz (to ) Tj 0.719444 0 Td 49.826389 Tz (increase ) Tj 2.547222 0 Td 49.691358 Tz (the ) Tj 1.030556 0 Td 54.282407 Tz (bulk ) Tj 1.438889 0 Td 59.953704 Tz (volume ) Tj 2.313889 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 51.851852 Tz (a ) Tj 0.486111 0 Td 55.092593 Tz (composite ) Tj 3.091667 0 Td 53.472222 Tz (and/or ) Tj 2.022222 0 Td 59.41358 Tz (add ) Tj 1.225 0 Td 51.851852 Tz (or ) Tj 51.428571 0 0 51.428571 0 2792.430925 Tm 6.358333 0 Td 44.074074 Tz (alter ) Tj 1.652778 0 Td 54.444444 Tz (mechanical ) Tj 3.577778 0 Td 48.611111 Tz (strengths ) Tj 2.975 0 Td 55.092593 Tz (of ) Tj 0.875 0 Td 50.771605 Tz (the ) Tj 1.244444 0 Td 52.5 Tz (composite; ) Tj 3.480556 0 Td 56.712963 Tz (examples ) Tj 3.072222 0 Td 56.712963 Tz (of ) Tj 51.428571 0 0 51.428571 0 2749.288462 Tm 6.377778 0 Td 54.282407 Tz (Such ) Tj 1.438889 0 Td 50.771605 Tz (are ) Tj 1.030556 0 Td 49.691358 Tz (the ) Tj 1.030556 0 Td 49.421296 Tz (addition ) Tj 2.508333 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 55.902778 Tz (sand ) Tj 1.477778 0 Td 58.333333 Tz (and ) Tj 1.166667 0 Td 50.231481 Tz (gravel ) Tj 1.944444 0 Td 52.211934 Tz (aggregate ) Tj 2.955556 0 Td 46.990741 Tz (to ) Tj 0.719444 0 Td 51.041667 Tz (concrete ) Tj 51.428571 0 0 51.428571 0 2708.346154 Tm 6.358333 0 Td 58.333333 Tz (and ) Tj 1.186111 0 Td 50.771605 Tz (the ) Tj 1.05 0 Td 49.826389 Tz (addition ) Tj 2.586111 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 54.552469 Tz (carbon ) Tj 2.158333 0 Td 50.925926 Tz (fiberaggregate ) Tj 4.433333 0 Td 48.611111 Tz (to ) Tj 0.738889 0 Td 55.092593 Tz (a ) Tj 0.447222 0 Td 59.259259 Tz (polymer ) Tj 2.605556 0 Td 83.179012 Tz (com ) Tj 51.428571 0 0 51.428571 0 2672 Tm 6.358333 0 Td 44.907407 Tz (posite. ) Tj 51.428571 0 0 51.428571 0 2623.390428 Tm 7.116667 0 Td 46.990741 Tz (In ) Tj 0.758333 0 Td 48.611111 Tz (the ) Tj 1.05 0 Td 49.691358 Tz (preferred ) Tj 2.858333 0 Td 58.038721 Tz (embodiment, ) Tj 4.005556 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 53.877315 Tz (diameter ) Tj 2.722222 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 51.851852 Tz (the ) Tj 1.108333 0 Td 49.016204 Tz (f-trical ) Tj 51.428571 0 0 51.428571 0 2585.075094 Tm 6.358333 0 Td 59.953704 Tz (cium ) Tj 1.730556 0 Td 55.092593 Tz (phosphate ) Tj 3.286111 0 Td 51.203704 Tz (aggregates ) Tj 3.402778 0 Td 51.851852 Tz (ranges ) Tj 2.197222 0 Td 57.523148 Tz (from ) Tj 1.672222 0 Td 57.87037 Tz (between ) Tj 2.819444 0 Td 56.481481 Tz (125-150 ) Tj 51.428571 0 0 51.428571 0 2542.064871 Tm 6.416667 0 Td 57.253086 Tz (um. ) Tj 1.244444 0 Td 64.814815 Tz (The ) Tj 1.4 0 Td 53.06713 Tz (diameter ) Tj 2.741667 0 Td 58.333333 Tz (of ) Tj 0.816667 0 Td 50.771605 Tz (the ) Tj 1.244444 0 Td 47.800926 Tz (B-tricalcium ) Tj 3.675 0 Td 55.092593 Tz (phosphate ) Tj 3.169444 0 Td 51.851852 Tz (aggregates ) Tj 51.428571 0 0 51.428571 0 2500.377309 Tm 6.358333 0 Td 55.092593 Tz (can ) Tj 1.244444 0 Td 59.953704 Tz (be ) Tj 0.952778 0 Td 50.636574 Tz (adjusted ) Tj 2.663889 0 Td 59.953704 Tz (by ) Tj 1.011111 0 Td 57.253086 Tz (one ) Tj 1.302778 0 Td 45.833333 Tz (skilled ) Tj 2.177778 0 Td 45.37037 Tz (in ) Tj 0.797222 0 Td 50.771605 Tz (the ) Tj 1.127778 0 Td 44.290123 Tz (art ) Tj 1.030556 0 Td 56.893004 Tz (depending ) Tj 3.305556 0 Td 58.333333 Tz (on ) Tj 0.972222 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 2460.619289 Tm 6.358333 0 Td 46.8107 Tz (injection ) Tj 2.955556 0 Td 52.391975 Tz (canula ) Tj 2.275 0 Td 50.968013 Tz (geometries. ) Tj 3.772222 0 Td 62.654321 Tz (The ) Tj 1.633333 0 Td 48.070988 Tz (B-tricalcium ) Tj 3.888889 0 Td 54.73251 Tz (phosphate ) Tj 51.428571 0 0 51.428571 0 2417.743652 Tm 6.358333 0 Td 52.314815 Tz (granule ) Tj 2.411111 0 Td 51.527778 Tz (aggregates ) Tj 3.325 0 Td 53.240741 Tz (provide ) Tj 2.469444 0 Td 55.092593 Tz (an ) Tj 0.913889 0 Td 52.283951 Tz (osteoconductive ) Tj 4.938889 0 Td 48.611111 Tz (Scaffold ) Tj 2.586111 0 Td 47.530864 Tz (for ) Tj 51.428571 0 0 51.428571 0 2377.072202 Tm 6.338889 0 Td 50.771605 Tz (the ) Tj 1.030556 0 Td 60.763889 Tz (bone ) Tj 1.613889 0 Td 54.768519 Tz (remodeling ) Tj 3.441667 0 Td 49.016204 Tz (process; ) Tj 2.508333 0 Td 51.851852 Tz (as ) Tj 0.758333 0 Td 50.771605 Tz (the ) Tj 1.030556 0 Td 53.877315 Tz (hydrogel ) Tj 2.761111 0 Td 38.888889 Tz (is ) Tj 0.641667 0 Td 49.331276 Tz (resorbed, ) Tj 2.819444 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 2336.785366 Tm 6.358333 0 Td 51.527778 Tz (aggregates ) Tj 3.286111 0 Td 50.771605 Tz (are ) Tj 1.088889 0 Td 57.407407 Tz (exposed ) Tj 2.586111 0 Td 46.990741 Tz (to ) Tj 0.777778 0 Td 50.771605 Tz (the ) Tj 1.108333 0 Td 53.324916 Tz (Surrounding ) Tj 3.713889 0 Td 59.41358 Tz (and ) Tj 1.263889 0 Td 52.256944 Tz (invading ) Tj 2.683333 0 Td 52.932099 Tz (tis ) Tj 51.428571 0 0 51.428571 0 2295.069212 Tm 6.377778 0 Td 46.180556 Tz (Sue. ) Tj 1.244444 0 Td 58.796296 Tz (Calcium ) Tj 2.625 0 Td 51.203704 Tz (containing ) Tj 3.208333 0 Td 46.296296 Tz (setting ) Tj 2.061111 0 Td 51.851852 Tz (agents ) Tj 2.022222 0 Td 68.055556 Tz (may ) Tj 1.361111 0 Td 58.333333 Tz (be ) Tj 0.816667 0 Td 61.574074 Tz (powder ) Tj 2.294444 0 Td 59.41358 Tz (and ) Tj 1.225 0 Td 51.851852 Tz (or ) Tj 51.428571 0 0 51.428571 0 2253.310924 Tm 6.358333 0 Td 46.548822 Tz (particulate ) Tj 3.266667 0 Td 51.851852 Tz (form. ) Tj 51.428571 0 0 51.428571 0 2210.243655 Tm 7.233333 0 Td 47.530864 Tz (B-tricalcium ) Tj 3.675 0 Td 54.73251 Tz (phosphate ) Tj 3.188889 0 Td 60.493827 Tz (Volume ) Tj 2.372222 0 Td 51.851852 Tz (percent ) Tj 2.411111 0 Td 56.712963 Tz (of ) Tj 0.816667 0 Td 50.771605 Tz (the ) Tj 1.147222 0 Td 55.452675 Tz (composite ) Tj 51.428571 0 0 51.428571 0 2169.767386 Tm 6.358333 0 Td 55.092593 Tz (can ) Tj 1.147222 0 Td 58.333333 Tz (be ) Tj 0.816667 0 Td 47.685185 Tz (altered ) Tj 2.119444 0 Td 46.990741 Tz (to ) Tj 0.719444 0 Td 53.06713 Tz (optimize ) Tj 2.683333 0 Td 52.777778 Tz (implant ) Tj 2.352778 0 Td 48.611111 Tz (for ) Tj 0.991667 0 Td 52.314815 Tz (optimal ) Tj 2.352778 0 Td 59.143519 Tz (bone ) Tj 1.575 0 Td 56.30787 Tz (regenera ) Tj 51.428571 0 0 51.428571 0 2125.074074 Tm 6.338889 0 Td 47.800926 Tz (tion ) Tj 1.380556 0 Td 48.316498 Tz (conditions, ) Tj 3.383333 0 Td 50.231481 Tz (as ) Tj 0.816667 0 Td 59.143519 Tz (bone ) Tj 1.633333 0 Td 52.314815 Tz (mineral ) Tj 2.411111 0 Td 49.537037 Tz (density ) Tj 2.313889 0 Td 46.990741 Tz (varies ) Tj 1.886111 0 Td 66.759259 Tz (among ) Tj 2.236111 0 Td 59.143519 Tz (loca ) Tj 40 0 0 40 0 2090 Tm 8.15 0 Td 60.833333 Tz (tions ) Tj 2.15 0 Td 65.972222 Tz (within ) Tj 2.65 0 Td 76.388889 Tz (and ) Tj 1.675 0 Td 73.214286 Tz (between ) Tj 3.375 0 Td 73.333333 Tz (bones ) Tj 2.5 0 Td 68.75 Tz (so ) Tj 1.125 0 Td 59.375 Tz (that ) Tj 1.7 0 Td 59.722222 Tz (different ) Tj 3.5 0 Td 65.277778 Tz (values ) Tj 2.65 0 Td 65.277778 Tz (are ) Tj 51.428571 0 0 51.428571 0 2043.677725 Tm 6.358333 0 Td 51.851852 Tz (presented ) Tj 2.936111 0 Td 57.253086 Tz (due ) Tj 1.127778 0 Td 46.990741 Tz (to ) Tj 0.7 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 51.388889 Tz (varying ) Tj 2.255556 0 Td 53.877315 Tz (presence ) Tj 2.722222 0 Td 50.231481 Tz (of ) Tj 0.680556 0 Td 51.203704 Tz (other ) Tj 1.652778 0 Td 53.240741 Tz (osseous ) Tj 2.372222 0 Td 47.685185 Tz (related ) Tj 40 0 0 40 0 2007 Tm 8.15 0 Td 60.416667 Tz (tissue ) Tj 2.425 0 Td 69.791667 Tz (Such ) Tj 1.9 0 Td 64.583333 Tz (as ) Tj 1.05 0 Td 64.015152 Tz (vasculature ) Tj 4.45 0 Td 75 Tz (and ) Tj 1.6 0 Td 71.428571 Tz (marrow. ) Tj 51.428571 0 0 51.428571 0 1959.60441 Tm 7.097222 0 Td 62.654321 Tz (The ) Tj 1.302778 0 Td 48.206019 Tz (alginate ) Tj 2.508333 0 Td 53.472222 Tz (hydrogel ) Tj 2.761111 0 Td 49.691358 Tz (serves ) Tj 2.002778 0 Td 48.611111 Tz (as ) Tj 0.797222 0 Td 51.851852 Tz (a ) Tj 0.525 0 Td 47.890947 Tz (diffusion ) Tj 2.780556 0 Td 46.759259 Tz (barrier ) Tj 2.119444 0 Td 48.611111 Tz (for ) Tj 1.069444 0 Td 48.611111 Tz (the ) Tj 51.428571 0 0 51.428571 0 1916.047382 Tm 6.358333 0 Td 48.148148 Tz (release ) Tj 2.313889 0 Td 56.712963 Tz (of ) Tj 0.913889 0 Td 48.971193 Tz (bioactive ) Tj 2.916667 0 Td 48.611111 Tz (agents, ) Tj 2.372222 0 Td 45.37037 Tz (carrier ) Tj 2.158333 0 Td 58.333333 Tz (of ) Tj 0.913889 0 Td 49.691358 Tz (the ) Tj 1.186111 0 Td 52.283951 Tz (osteoconductive ) Tj 51.428571 0 0 51.428571 0 1877.566265 Tm 6.358333 0 Td 51.527778 Tz (aggregates ) Tj 3.266667 0 Td 60.493827 Tz (and ) Tj 1.225 0 Td 49.331276 Tz (bioactive ) Tj 2.8 0 Td 51.851852 Tz (agents ) Tj 2.041667 0 Td 46.990741 Tz (to ) Tj 0.758333 0 Td 49.691358 Tz (the ) Tj 1.069444 0 Td 53.240741 Tz (implant ) Tj 2.391667 0 Td 39.537037 Tz (site, ) Tj 1.361111 0 Td 47.170782 Tz (localizes ) Tj 51.428571 0 0 51.428571 0 1836.078624 Tm 6.338889 0 Td 50.771605 Tz (the ) Tj 1.186111 0 Td 53.240741 Tz (implant ) Tj 2.508333 0 Td 59.41358 Tz (and ) Tj 1.361111 0 Td 52.067901 Tz (osteoconductive ) Tj 4.958333 0 Td 49.494949 Tz (aggregates, ) Tj 3.538889 0 Td 60.493827 Tz (and ) Tj 1.361111 0 Td 53.472222 Tz (degrades ) Tj 51.428571 0 0 51.428571 0 1795.014652 Tm 6.358333 0 Td 45.046296 Tz (relatively ) Tj 2.897222 0 Td 47.962963 Tz (early ) Tj 1.613889 0 Td 45.37037 Tz (after ) Tj 1.497222 0 Td 49.358974 Tz (implantation, ) Tj 4.044444 0 Td 54.282407 Tz (such ) Tj 1.497222 0 Td 48.611111 Tz (as ) Tj 0.758333 0 Td 59.41358 Tz (one ) Tj 1.205556 0 Td 48.611111 Tz (to ) Tj 0.777778 0 Td 51.041667 Tz (four ) Tj 1.361111 0 Td 60.277778 Tz (weeks ) Tj 51.428571 0 0 51.428571 0 1753.831373 Tm 6.358333 0 Td 45.37037 Tz (after ) Tj 1.711111 0 Td 49.109687 Tz (implantation. ) Tj 4.2 0 Td 64.814815 Tz (As ) Tj 1.166667 0 Td 50.771605 Tz (the ) Tj 1.263889 0 Td 54.282407 Tz (hydrogel ) Tj 2.994444 0 Td 53.06713 Tz (degrades ) Tj 2.936111 0 Td 49.691358 Tz (the ) Tj 1.283333 0 Td 54.012346 Tz (slower ) Tj 51.428571 0 0 51.428571 0 1712.437884 Tm 6.358333 0 Td 53.652263 Tz (degrading ) Tj 3.208333 0 Td 52.067901 Tz (osteoconductive ) Tj 4.958333 0 Td 51.203704 Tz (aggregates ) Tj 3.344444 0 Td 50.771605 Tz (are ) Tj 1.186111 0 Td 51.851852 Tz (presented ) Tj 3.072222 0 Td 45.37037 Tz (at ) Tj 0.816667 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 1670.403402 Tm 6.338889 0 Td 54.282407 Tz (hydrogel ) Tj 2.8 0 Td 51.557239 Tz (degradation ) Tj 3.636111 0 Td 43.209877 Tz (front, ) Tj 1.788889 0 Td 46.666667 Tz (ideal ) Tj 1.613889 0 Td 47.530864 Tz (for ) Tj 1.030556 0 Td 49.691358 Tz (the ) Tj 1.127778 0 Td 51.851852 Tz ("creeping ) Tj 2.994444 0 Td 51.851852 Tz (Substitu ) Tj 52.857143 0 0 52.857143 0 1625.653563 Tm 6.167568 0 Td 47.297297 Tz (tion ) Tj 1.645946 0 Td 48.873874 Tz (nature ) Tj 1.92973 0 Td 56.756757 Tz (of ) Tj 0.813514 0 Td 48.611111 Tz (regenerating ) Tj 3.67027 0 Td 57.545045 Tz (bone ) Tj 1.57027 0 Td 51.238739 Tz (with ) Tj 1.418919 0 Td 58.333333 Tz (bone ) Tj 1.57027 0 Td 44.66967 Tz (grafts ) Tj 1.797297 0 Td 57.807808 Tz (and ) Tj 1.22973 0 Td 57.545045 Tz (bone ) Tj 51.428571 0 0 51.428571 0 1584.396624 Tm 6.358333 0 Td 46.018519 Tz (graft ) Tj 1.555556 0 Td 44.830247 Tz (Substitutes. ) Tj 51.428571 0 0 51.428571 0 1545.226804 Tm 7.097222 0 Td 62.654321 Tz (The ) Tj 1.302778 0 Td 54.282407 Tz (hydrogel ) Tj 2.819444 0 Td 38.888889 Tz (is ) Tj 0.7 0 Td 55.092593 Tz (an ) Tj 0.875 0 Td 48.611111 Tz (alginate ) Tj 2.547222 0 Td 48.206019 Tz (Solution ) Tj 2.547222 0 Td 61.168981 Tz (composed ) Tj 3.169444 0 Td 55.092593 Tz (of ) Tj 0.777778 0 Td 47.222222 Tz (alginic ) Tj 51.428571 0 0 51.428571 0 1500.980676 Tm 6.358333 0 Td 45.37037 Tz (acid. ) Tj 1.497222 0 Td 64.814815 Tz (The ) Tj 1.263889 0 Td 47.222222 Tz (alginic ) Tj 2.119444 0 Td 51.851852 Tz (acid ) Tj 1.361111 0 Td 40.509259 Tz (is ) Tj 0.661111 0 Td 64.814815 Tz (made ) Tj 1.672222 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 55.092593 Tz (a ) Tj 0.505556 0 Td 56.388889 Tz (monovalent ) Tj 3.5 0 Td 42.777778 Tz (salts ) Tj 1.438889 0 Td 53.472222 Tz (of ) Tj 0.7 0 Td 47.685185 Tz (alginic ) Tj 51.428571 0 0 51.428571 0 1462.001209 Tm 6.358333 0 Td 46.018519 Tz (acid, ) Tj 1.594444 0 Td 53.472222 Tz (Such ) Tj 1.458333 0 Td 50.231481 Tz (as ) Tj 0.797222 0 Td 58.873457 Tz (Sodium ) Tj 2.313889 0 Td 48.206019 Tz (alginate ) Tj 2.469444 0 Td 53.472222 Tz (or ) Tj 0.758333 0 Td 54.372428 Tz (potassium ) Tj 3.111111 0 Td 49.016204 Tz (alginate ) Tj 2.547222 0 Td 58.333333 Tz (Polymer ) Tj 51.428571 0 0 51.428571 0 1418.688243 Tm 6.338889 0 Td 56.17284 Tz (weight ) Tj 2.313889 0 Td 51.851852 Tz (percent ) Tj 2.488889 0 Td 59.953704 Tz (of ) Tj 0.952778 0 Td 48.611111 Tz (the ) Tj 1.186111 0 Td 53.877315 Tz (hydrogel ) Tj 2.916667 0 Td 56.17284 Tz (can ) Tj 1.322222 0 Td 59.953704 Tz (be ) Tj 1.05 0 Td 47.222222 Tz (altered ) Tj 2.294444 0 Td 46.990741 Tz (to ) Tj 0.894444 0 Td 49.074074 Tz (control ) Tj 40 0 0 40 0 1382 Tm 8.175 0 Td 61.904762 Tz (release ) Tj 2.825 0 Td 57.87037 Tz (kinetics. ) Tj 51.428571 0 0 51.428571 0 1335.447073 Tm 7.097222 0 Td 57.87037 Tz (Calcium ) Tj 2.586111 0 Td 55.092593 Tz (phosphate ) Tj 3.130556 0 Td 49.691358 Tz (serves ) Tj 1.963889 0 Td 48.611111 Tz (as ) Tj 0.758333 0 Td 48.611111 Tz (the ) Tj 1.069444 0 Td 48.206019 Tz (scaffold ) Tj 2.45 0 Td 49.691358 Tz (for ) Tj 1.011111 0 Td 52.121914 Tz (angiogenesis ) Tj 51.428571 0 0 51.428571 0 1293.730769 Tm 6.358333 0 Td 58.333333 Tz (and ) Tj 1.205556 0 Td 49.109687 Tz (osteogenesis. ) Tj 4.005556 0 Td 58.796296 Tz (Calcium ) Tj 2.644444 0 Td 45.833333 Tz (Sulfate ) Tj 2.080556 0 Td 50.231481 Tz (serves ) Tj 1.963889 0 Td 50.231481 Tz (as ) Tj 0.758333 0 Td 50.771605 Tz (the ) Tj 1.088889 0 Td 56.388889 Tz (major ) Tj 1.847222 0 Td 52.391975 Tz (source ) Tj 51.428571 0 0 51.428571 0 1249.97417 Tm 6.358333 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 55.092593 Tz (calcium ) Tj 2.527778 0 Td 50.231481 Tz (ions ) Tj 1.4 0 Td 46.990741 Tz (to ) Tj 0.777778 0 Td 54.012346 Tz (induce ) Tj 2.119444 0 Td 50.771605 Tz (the ) Tj 1.127778 0 Td 45.833333 Tz (setting ) Tj 2.138889 0 Td 46.018519 Tz (reactions. ) Tj 2.936111 0 Td 64.814815 Tz (The ) Tj 1.361111 0 Td 55.092593 Tz (calcium ) Tj 51.428571 0 0 51.428571 0 1214.714451 Tm 6.358333 0 Td 60.133745 Tz (component ) Tj 3.344444 0 Td 52.256944 Tz (provides ) Tj 2.644444 0 Td 58.333333 Tz (a ) Tj 0.466667 0 Td 49.945534 Tz (tissue-engineered ) Tj 5.230556 0 Td 49.494949 Tz (scaffolding ) Tj 3.422222 0 Td 49.016204 Tz (material ) Tj 51.428571 0 0 51.428571 0 1170.964539 Tm 6.358333 0 Td 48.611111 Tz (for ) Tj 0.952778 0 Td 52.391975 Tz (angiogenesis ) Tj 3.908333 0 Td 59.41358 Tz (and ) Tj 1.205556 0 Td 48.860399 Tz (osteogenesis. ) Tj 4.025 0 Td 54.012346 Tz (For ) Tj 1.088889 0 Td 54.6875 Tz (example, ) Tj 2.780556 0 Td 53.703704 Tz (calcium ) Tj 2.430556 0 Td 59.41358 Tz (Sul ) Tj 51.428571 0 0 51.428571 0 1126.973717 Tm 6.358333 0 Td 45.37037 Tz (fate ) Tj 1.283333 0 Td 55.092593 Tz (can ) Tj 1.205556 0 Td 56.712963 Tz (be ) Tj 0.855556 0 Td 58.333333 Tz (added ) Tj 1.944444 0 Td 45.37037 Tz (to ) Tj 0.738889 0 Td 50.771605 Tz (the ) Tj 1.127778 0 Td 53.914141 Tz (composition ) Tj 3.752778 0 Td 46.990741 Tz (to ) Tj 0.777778 0 Td 52.777778 Tz (provide ) Tj 2.372222 0 Td 50.771605 Tz (the ) Tj 1.108333 0 Td 55.092593 Tz (calcium ) Tj 51.428571 0 0 51.428571 0 1087.788798 Tm 6.358333 0 Td 56.388889 Tz (component. ) Tj 3.538889 0 Td 46.990741 Tz (Similarly, ) Tj 2.936111 0 Td 54.62963 Tz (calcium ) Tj 2.411111 0 Td 46.296296 Tz (sulfate ) Tj 2.022222 0 Td 52.256944 Tz (provides ) Tj 2.605556 0 Td 58.333333 Tz (a ) Tj 0.466667 0 Td 52.662037 Tz (high ) Tj 1.4 0 Td 50 Tz (Surface ) Tj 40 0 0 40 0 1049 Tm 8.175 0 Td 65.625 Tz (area ) Tj 1.8 0 Td 62.5 Tz (for ) Tj 1.325 0 Td 69.047619 Tz (calcium ) Tj 3.175 0 Td 61.742424 Tz (dissolution ) Tj 4.325 0 Td 59.375 Tz (into ) Tj 1.675 0 Td 63.888889 Tz (the ) Tj 1.4 0 Td 62.5 Tz (matrix. ) Tj 2.875 0 Td 75 Tz (Calcium ) Tj 3.425 0 Td 58.928571 Tz (sulfate ) Tj 51.428571 0 0 51.428571 0 1002.360494 Tm 6.358333 0 Td 48.971193 Tz (dissolves ) Tj 2.877778 0 Td 65.625 Tz (much ) Tj 1.788889 0 Td 45.37037 Tz (faster ) Tj 1.788889 0 Td 59.41358 Tz (and ) Tj 1.263889 0 Td 52.932099 Tz (has ) Tj 1.147222 0 Td 58.333333 Tz (a ) Tj 0.563889 0 Td 65.625 Tz (much ) Tj 1.769444 0 Td 52.932099 Tz (higher ) Tj 2.061111 0 Td 45.694444 Tz (solubility ) Tj 2.936111 0 Td 54.282407 Tz (than ) Tj 51.428571 0 0 51.428571 0 962.634921 Tm 6.358333 0 Td 55.092593 Tz (does ) Tj 1.730556 0 Td 54.166667 Tz (calcium ) Tj 2.683333 0 Td 55.092593 Tz (phosphate ) Tj 3.344444 0 Td 46.990741 Tz (\(for ) Tj 1.497222 0 Td 52.572016 Tz (example\). ) Tj 3.286111 0 Td 46.990741 Tz (In ) Tj 0.972222 0 Td 49.691358 Tz (the ) Tj 1.283333 0 Td 50.05144 Tz (preferred ) Tj 48.571429 0 0 48.571429 0 919 Tm 6.732353 0 Td 60.828877 Tz (embodiment, ) Tj 4.302941 0 Td 56.862745 Tz (calcium ) Tj 2.676471 0 Td 39.460784 Tz (is ) Tj 0.741176 0 Td 54.411765 Tz (derived ) Tj 2.511765 0 Td 60.906863 Tz (from ) Tj 1.708824 0 Td 61.764706 Tz (a ) Tj 0.617647 0 Td 57.352941 Tz (calcium ) Tj 2.655882 0 Td 45.465686 Tz (salt ) Tj 1.317647 0 Td 55.473856 Tz (and/or ) Tj 51.428571 0 0 51.428571 0 878.16707 Tm 6.338889 0 Td 55.092593 Tz (base ) Tj 1.458333 0 Td 55.092593 Tz (having ) Tj 2.119444 0 Td 58.333333 Tz (an ) Tj 0.855556 0 Td 52.572016 Tz (elemental ) Tj 3.013889 0 Td 54.166667 Tz (calcium ) Tj 2.469444 0 Td 59.143519 Tz (mass ) Tj 1.594444 0 Td 46.990741 Tz (in ) Tj 0.719444 0 Td 58.981481 Tz (grams ) Tj 1.925 0 Td 48.611111 Tz (to ) Tj 0.719444 0 Td 54.282407 Tz (hydrogel ) Tj 51.428571 0 0 51.428571 0 833.935961 Tm 6.358333 0 Td 60.493827 Tz (volume ) Tj 2.333333 0 Td 45.37037 Tz (in ) Tj 0.758333 0 Td 41.049383 Tz (milliliters, ) Tj 3.15 0 Td 55.555556 Tz (wherein ) Tj 2.508333 0 Td 51.851852 Tz (a ) Tj 0.505556 0 Td 43.425926 Tz (ratio ) Tj 1.516667 0 Td 51.311728 Tz (ranges ) Tj 2.022222 0 Td 58.333333 Tz (from ) Tj 1.575 0 Td 56.018519 Tz (0.00238 ) Tj 2.527778 0 Td 46.990741 Tz (to ) Tj 51.428571 0 0 51.428571 0 795.182692 Tm 6.358333 0 Td 54.79798 Tz (0.00076824. ) Tj 3.830556 0 Td 48.361823 Tz (Additionally, ) Tj 4.005556 0 Td 45.37037 Tz (in ) Tj 0.777778 0 Td 48.611111 Tz (the ) Tj 1.069444 0 Td 49.331276 Tz (preferred ) Tj 2.877778 0 Td 57.744108 Tz (embodiment, ) Tj 4.025 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 751.50828 Tm 6.358333 0 Td 54.166667 Tz (calcium ) Tj 2.605556 0 Td 52.932099 Tz (source ) Tj 2.197222 0 Td 38.888889 Tz (is ) Tj 0.777778 0 Td 50.925926 Tz (derived ) Tj 2.430556 0 Td 58.333333 Tz (from ) Tj 1.730556 0 Td 53.703704 Tz (calcium ) Tj 2.566667 0 Td 46.296296 Tz (sulfate ) Tj 2.255556 0 Td 47.962963 Tz (dihydrate. ) Tj 51.428571 0 0 51.428571 0 710.382716 Tm 6.358333 0 Td 58.333333 Tz (Calcium ) Tj 2.683333 0 Td 45.37037 Tz (sulfate ) Tj 2.138889 0 Td 50.411523 Tz (dihydrate ) Tj 2.916667 0 Td 54.012346 Tz (has ) Tj 1.147222 0 Td 59.953704 Tz (an ) Tj 0.913889 0 Td 52.211934 Tz (elemental ) Tj 3.033333 0 Td 53.703704 Tz (calcium ) Tj 2.527778 0 Td 59.143519 Tz (mass ) Tj 1.613889 0 Td 40.509259 Tz (is ) Tj 51.428571 0 0 51.428571 0 669.807799 Tm 6.358333 0 Td 54.444444 Tz (23.28 ) Tj 1.827778 0 Td 50.925926 Tz (percent ) Tj 2.313889 0 Td 59.953704 Tz (of ) Tj 0.797222 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 54.62963 Tz (calcium ) Tj 2.508333 0 Td 45.833333 Tz (sulfate ) Tj 2.1 0 Td 51.131687 Tz (dihydrate ) Tj 2.955556 0 Td 53.148148 Tz (mass. ) Tj 51.428571 0 0 51.428571 0 630.470588 Tm 7.097222 0 Td 58.981481 Tz (Alpha ) Tj 1.944444 0 Td 57.87037 Tz (hydroxy ) Tj 2.644444 0 Td 50.87963 Tz (carboxylic ) Tj 3.247222 0 Td 49.907407 Tz (acids ) Tj 1.730556 0 Td 51.851852 Tz (or ) Tj 0.797222 0 Td 42.12963 Tz (salts ) Tj 1.477778 0 Td 51.388889 Tz (thereof ) Tj 2.275 0 Td 69.135802 Tz (may ) Tj 1.438889 0 Td 48.611111 Tz (also ) Tj 51.428571 0 0 51.428571 0 589.431629 Tm 6.338889 0 Td 59.953704 Tz (be ) Tj 0.894444 0 Td 50.771605 Tz (incorporated ) Tj 3.791667 0 Td 46.990741 Tz (to ) Tj 0.738889 0 Td 57.407407 Tz (improve ) Tj 2.586111 0 Td 46.843434 Tz (flowability ) Tj 3.247222 0 Td 60.493827 Tz (and ) Tj 1.244444 0 Td 46.296296 Tz (setting ) Tj 2.1 0 Td 53.292181 Tz (character ) Tj 51.428571 0 0 51.428571 0 542.979592 Tm 6.358333 0 Td 41.049383 Tz (istics ) Tj 1.75 0 Td 45.37037 Tz (in ) Tj 0.797222 0 Td 64.814815 Tz (some ) Tj 1.788889 0 Td 56.712963 Tz (embodiments, ) Tj 4.316667 0 Td 51.851852 Tz (as ) Tj 0.875 0 Td 51.851852 Tz (a ) Tj 0.583333 0 Td 53.472222 Tz (slower ) Tj 2.138889 0 Td 51.851852 Tz (curing ) Tj 2.119444 0 Td 49.421296 Tz (material ) Tj 2.605556 0 Td 40.509259 Tz (is ) Tj 51.428571 0 0 51.428571 0 505.523039 Tm 6.358333 0 Td 48.070988 Tz (easier ) Tj 1.925 0 Td 43.75 Tz (to ) Tj 0.777778 0 Td 41.666667 Tz (inject. ) Tj 1.963889 0 Td 63.734568 Tz (The ) Tj 1.322222 0 Td 53.148148 Tz (alpha ) Tj 1.808333 0 Td 57.407407 Tz (hydroxy ) Tj 2.605556 0 Td 51.203704 Tz (carboxylic ) Tj 3.305556 0 Td 55.092593 Tz (can ) Tj 1.205556 0 Td 56.712963 Tz (be ) Tj 0.875 0 Td 47.530864 Tz (either ) Tj 40 0 0 40 0 467 Tm 8.2 0 Td 61.979167 Tz (Solution ) Tj 3.225 0 Td 68.75 Tz (or ) Tj 1.025 0 Td 54.166667 Tz (salt ) Tj 1.55 0 Td 66.666667 Tz (form. ) Tj 51.428571 0 0 51.428571 0 421.753604 Tm 7.116667 0 Td 51.131687 Tz (Bioactive ) Tj 2.936111 0 Td 51.311728 Tz (agents ) Tj 2.1 0 Td 65.895062 Tz (may ) Tj 1.380556 0 Td 59.953704 Tz (be ) Tj 0.894444 0 Td 58.981481 Tz (added ) Tj 1.983333 0 Td 52.391975 Tz (during ) Tj 2.1 0 Td 56.712963 Tz (mixing ) Tj 2.255556 0 Td 47.530864 Tz (for ) Tj 1.05 0 Td 43.75 Tz (in ) Tj 0.758333 0 Td 51.851852 Tz (vivo ) Tj 51.428571 0 0 51.428571 0 378.651163 Tm 6.358333 0 Td 49.421296 Tz (delivery ) Tj 2.469444 0 Td 59.41358 Tz (and ) Tj 1.186111 0 Td 49.259259 Tz (controlled ) Tj 3.052778 0 Td 48.611111 Tz (release ) Tj 2.138889 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 48.021886 Tz (bio-agents. ) Tj 3.305556 0 Td 62.654321 Tz (The ) Tj 1.244444 0 Td 50.084175 Tz (biomaterial ) Tj 51.428571 0 0 51.428571 0 339.895377 Tm 6.358333 0 Td 55.452675 Tz (composite ) Tj 3.130556 0 Td 40.509259 Tz (is ) Tj 0.661111 0 Td 53.472222 Tz (designed ) Tj 2.683333 0 Td 48.611111 Tz (to ) Tj 0.738889 0 Td 56.712963 Tz (be ) Tj 0.836111 0 Td 59.62963 Tz (mixed ) Tj 1.963889 0 Td 43.75 Tz (in ) Tj 0.661111 0 Td 58.333333 Tz (a ) Tj 0.486111 0 Td 42.592593 Tz (sterile ) Tj 1.925 0 Td 47.800926 Tz (Surgical ) Tj 2.469444 0 Td 45.833333 Tz (setting ) Tj 51.428571 0 0 51.428571 0 295.745198 Tm 6.358333 0 Td 58.333333 Tz (and ) Tj 1.302778 0 Td 49.691358 Tz (delivered ) Tj 2.916667 0 Td 48.611111 Tz (as ) Tj 0.836111 0 Td 51.851852 Tz (a ) Tj 0.583333 0 Td 46.450617 Tz (Sol ) Tj 1.05 0 Td 54.166667 Tz (through ) Tj 2.527778 0 Td 55.092593 Tz (a ) Tj 0.602778 0 Td 54.372428 Tz (minimally ) Tj 3.208333 0 Td 49.016204 Tz (invasive ) Tj 2.605556 0 Td 60.925926 Tz (means ) Tj 51.428571 0 0 51.428571 0 253.201274 Tm 6.377778 0 Td 45.664983 Tz (\(injection\) ) Tj 3.227778 0 Td 46.990741 Tz (to ) Tj 0.797222 0 Td 56.712963 Tz (an ) Tj 0.913889 0 Td 53.703704 Tz (osseous ) Tj 2.508333 0 Td 41.319444 Tz (site ) Tj 1.205556 0 Td 59.62963 Tz (where ) Tj 2.002778 0 Td 34.027778 Tz (it ) Tj 0.602778 0 Td 49.421296 Tz (gels ) Tj 1.438889 0 Td 47.530864 Tz (for ) Tj 1.069444 0 Td 47.314815 Tz (intentions ) Tj 3.091667 0 Td 56.712963 Tz (of ) Tj 51.428571 0 0 51.428571 0 212.647343 Tm 6.358333 0 Td 48.070988 Tz (repair ) Tj 1.905556 0 Td 52.932099 Tz (and/or ) Tj 2.061111 0 Td 49.96142 Tz (regeneration ) Tj 3.811111 0 Td 58.333333 Tz (of ) Tj 0.797222 0 Td 59.953704 Tz (bone ) Tj 1.613889 0 Td 59.41358 Tz (and ) Tj 1.263889 0 Td 37.808642 Tz (its ) Tj 0.855556 0 Td 50.87963 Tz (associated ) Tj 3.227778 0 Td 45.833333 Tz (tissues ) Tj 51.428571 0 0 51.428571 0 171.794994 Tm 6.377778 0 Td 54.282407 Tz (\(marrow, ) Tj 2.8 0 Td 48.070988 Tz (vasculature, ) Tj 3.636111 0 Td 52.391975 Tz (and/or ) Tj 2.041667 0 Td 50.231481 Tz (adjacent ) Tj 2.625 0 Td 44.074074 Tz (cartilage, ) Tj 2.858333 0 Td 49.691358 Tz (ligament, ) Tj 2.897222 0 Td 51.851852 Tz (or ) Tj 30 0 0 30 0 2755 Tm 41.833333 0 Td 72.222222 Tz (5 ) Tj 30 0 0 30 0 2547 Tm 41.666667 0 Td 75 Tz (10 ) Tj 30 0 0 30 0 2339 Tm 41.666667 0 Td 72.222222 Tz (15 ) Tj 30 0 0 30 0 1923 Tm 41.6 0 Td 77.777778 Tz (25 ) Tj 30 0 0 30 0 1716 Tm 41.6 0 Td 80.555556 Tz (30 ) Tj 30 0 0 30 0 1507 Tm 41.6 0 Td 77.777778 Tz (35 ) Tj 30 0 0 30 0 1299 Tm 41.566667 0 Td 83.333333 Tz (40 ) Tj 30 0 0 30 0 1091 Tm 41.566667 0 Td 80.555556 Tz (45 ) Tj 30 0 0 30 0 883 Tm 41.6 0 Td 80.555556 Tz (50 ) Tj 30 0 0 30 0 675 Tm 41.6 0 Td 77.777778 Tz (55 ) Tj 30 0 0 30 0 467 Tm 41.633333 0 Td 77.777778 Tz (60 ) Tj 30 0 0 30 0 259 Tm 41.633333 0 Td 75 Tz (65 ) Tj 52.857143 0 0 52.857143 0 2968 Tm 32.881081 0 Td 72.522523 Tz (8 ) Tj 51.428571 0 0 51.428571 0 2914.256545 Tm 25.277778 0 Td 49.826389 Tz (tendon\). ) Tj 2.702778 0 Td 53.472222 Tz (This ) Tj 1.575 0 Td 59.953704 Tz (bone ) Tj 1.691667 0 Td 48.611111 Tz (grafting ) Tj 2.644444 0 Td 49.016204 Tz (material ) Tj 2.644444 0 Td 56.17284 Tz (can ) Tj 1.283333 0 Td 49.421296 Tz (also ) Tj 1.497222 0 Td 56.712963 Tz (be ) Tj 0.991667 0 Td 55.092593 Tz (used ) Tj 1.633333 0 Td 47.530864 Tz (for ) Tj 51.428571 0 0 51.428571 0 2879.026764 Tm 25.297222 0 Td 48.420479 Tz (osseointegration, ) Tj 5.172222 0 Td 55.092593 Tz (such ) Tj 1.555556 0 Td 51.851852 Tz (as ) Tj 0.875 0 Td 50.636574 Tz (required ) Tj 2.683333 0 Td 52.391975 Tz (during ) Tj 2.1 0 Td 48.341049 Tz (arthroplasty ) Tj 3.752778 0 Td 43.425926 Tz (\(hip, ) Tj 51.428571 0 0 51.428571 0 2831.354601 Tm 25.297222 0 Td 51.851852 Tz (knee, ) Tj 1.75 0 Td 44.722222 Tz (disc, ) Tj 1.516667 0 Td 49.151235 Tz (ankle, ) Tj 1.983333 0 Td 57.037037 Tz (elbow ) Tj 1.944444 0 Td 58.333333 Tz (and ) Tj 1.263889 0 Td 48.287037 Tz (shoulder\), ) Tj 3.130556 0 Td 48.611111 Tz (dental ) Tj 1.944444 0 Td 49.691358 Tz (implants, ) Tj 2.858333 0 Td 60.493827 Tz (and ) Tj 51.428571 0 0 51.428571 0 2791.728248 Tm 25.297222 0 Td 53.472222 Tz (attachment ) Tj 3.383333 0 Td 49.826389 Tz (\(tendon, ) Tj 2.605556 0 Td 50.411523 Tz (ligament, ) Tj 2.916667 0 Td 46.450617 Tz (cartilage ) Tj 2.722222 0 Td 49.421296 Tz (\(hyaline ) Tj 2.527778 0 Td 60.493827 Tz (and ) Tj 1.263889 0 Td 42.12963 Tz (fibro\)\). ) Tj 51.428571 0 0 51.428571 0 2750.398058 Tm 25.297222 0 Td 48.860399 Tz (Additionally, ) Tj 4.005556 0 Td 48.611111 Tz (the ) Tj 1.030556 0 Td 50.378788 Tz (biomaterial ) Tj 3.519444 0 Td 55.452675 Tz (composite ) Tj 3.169444 0 Td 55.092593 Tz (can ) Tj 1.186111 0 Td 58.333333 Tz (be ) Tj 0.894444 0 Td 55.092593 Tz (used ) Tj 1.497222 0 Td 48.611111 Tz (for ) Tj 1.030556 0 Td 62.654321 Tz (ath ) Tj 51.428571 0 0 51.428571 0 2706.512195 Tm 25.297222 0 Td 46.585648 Tz (rodesis, ) Tj 2.45 0 Td 54.282407 Tz (such ) Tj 1.497222 0 Td 50.231481 Tz (as ) Tj 0.797222 0 Td 48.935185 Tz (athrodesis ) Tj 3.130556 0 Td 50.231481 Tz (across ) Tj 2.022222 0 Td 46.990741 Tz (diarthrodial ) Tj 3.538889 0 Td 45.37037 Tz (joints ) Tj 1.866667 0 Td 55.092593 Tz (of ) Tj 0.758333 0 Td 46.018519 Tz (hips, ) Tj 51.428571 0 0 51.428571 0 2667.270886 Tm 25.297222 0 Td 50.771605 Tz (knees, ) Tj 2.138889 0 Td 44.907407 Tz (wrists, ) Tj 2.177778 0 Td 59.41358 Tz (and ) Tj 1.322222 0 Td 48.611111 Tz (ankles, ) Tj 2.352778 0 Td 51.851852 Tz (or ) Tj 0.855556 0 Td 51.419753 Tz (amphiarthrodial ) Tj 4.9 0 Td 45.910494 Tz (joints ) Tj 1.944444 0 Td 59.953704 Tz (of ) Tj 0.875 0 Td 50.771605 Tz (the ) Tj 51.428571 0 0 51.428571 0 2630 Tm 25.316667 0 Td 46.450617 Tz (spine. ) Tj 51.428571 0 0 51.428571 0 2586.032765 Tm 26.016667 0 Td 63.734568 Tz (The ) Tj 1.361111 0 Td 54.282407 Tz (term ) Tj 1.575 0 Td 51.311728 Tz (angiogenic ) Tj 4.044444 0 Td 60.277778 Tz (means ) Tj 2.061111 0 Td 55.092593 Tz (a ) Tj 0.544444 0 Td 53.148148 Tz (agent ) Tj 1.808333 0 Td 52.777778 Tz (causing ) Tj 2.411111 0 Td 50.771605 Tz (the ) Tj 1.108333 0 Td 60.925926 Tz (physi ) Tj 51.428571 0 0 51.428571 0 2543.696078 Tm 25.297222 0 Td 48.611111 Tz (ological ) Tj 2.508333 0 Td 52.314815 Tz (process ) Tj 2.372222 0 Td 51.131687 Tz (involving ) Tj 2.916667 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 56.17284 Tz (growth ) Tj 2.275 0 Td 55.092593 Tz (of ) Tj 0.797222 0 Td 61.574074 Tz (new ) Tj 1.322222 0 Td 55.740741 Tz (blood ) Tj 1.866667 0 Td 45.775463 Tz (vessels. ) Tj 51.428571 0 0 51.428571 0 2497.616993 Tm 26.016667 0 Td 63.734568 Tz (The ) Tj 1.361111 0 Td 55.092593 Tz (term ) Tj 1.575 0 Td 53.472222 Tz (annulus ) Tj 2.838889 0 Td 47.890947 Tz (fibrosus' ) Tj 2.858333 0 Td 60.925926 Tz (means ) Tj 2.061111 0 Td 47.685185 Tz (fibrous ) Tj 2.275 0 Td 47.800926 Tz (ring ) Tj 1.380556 0 Td 59.953704 Tz (of ) Tj 0.836111 0 Td 51.851852 Tz (inter ) Tj 51.428571 0 0 51.428571 0 2458.401022 Tm 25.297222 0 Td 47.530864 Tz (vertebral ) Tj 2.916667 0 Td 44.074074 Tz (disc; ) Tj 1.711111 0 Td 48.611111 Tz (the ) Tj 1.166667 0 Td 49.475309 Tz (circumferential ) Tj 4.841667 0 Td 45.730453 Tz (ring-like ) Tj 2.761111 0 Td 50 Tz (portion ) Tj 2.45 0 Td 59.953704 Tz (of ) Tj 0.933333 0 Td 59.953704 Tz (an ) Tj 40 0 0 40 0 2422 Tm 32.525 0 Td 59.821429 Tz (intervertebral ) Tj 5.275 0 Td 56.666667 Tz (disc. ) Tj 51.428571 0 0 51.428571 0 2376.960591 Tm 26.016667 0 Td 63.734568 Tz (The ) Tj 1.244444 0 Td 55.902778 Tz (term ) Tj 1.516667 0 Td 46.018519 Tz (articular ) Tj 2.877778 0 Td 46.666667 Tz (cartilage' ) Tj 2.955556 0 Td 60.925926 Tz (means ) Tj 1.963889 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 46.090535 Tz (cartilage ) Tj 2.625 0 Td 52.662037 Tz (covering ) Tj 51.428571 0 0 51.428571 0 2332.840964 Tm 25.277778 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 45.37037 Tz (articular ) Tj 2.586111 0 Td 49.421296 Tz (surfaces ) Tj 2.547222 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 56.388889 Tz (bones ) Tj 1.847222 0 Td 47.61396 Tz (participating ) Tj 3.888889 0 Td 43.75 Tz (in ) Tj 0.680556 0 Td 58.333333 Tz (a ) Tj 0.505556 0 Td 53.06713 Tz (diarthro ) Tj 51.428571 0 0 51.428571 0 2298 Tm 25.297222 0 Td 47.863248 Tz (dial/synovial ) Tj 3.869444 0 Td 43.209877 Tz (joint. ) Tj 51.428571 0 0 51.428571 0 2251.636364 Tm 26.016667 0 Td 63.734568 Tz (The ) Tj 1.4 0 Td 55.092593 Tz (term ) Tj 1.594444 0 Td 51.851852 Tz ("hyaline ) Tj 2.741667 0 Td 46.990741 Tz (cartilage' ) Tj 3.111111 0 Td 66.975309 Tz (may ) Tj 1.497222 0 Td 58.333333 Tz (be ) Tj 0.972222 0 Td 55.092593 Tz (used ) Tj 1.594444 0 Td 45.37037 Tz (to ) Tj 0.816667 0 Td 50.636574 Tz (describe ) Tj 2.683333 0 Td 58.333333 Tz (a ) Tj 51.428571 0 0 51.428571 0 2209.123623 Tm 25.297222 0 Td 46.090535 Tz (cartilage ) Tj 2.644444 0 Td 58.333333 Tz (which ) Tj 1.905556 0 Td 38.888889 Tz (is ) Tj 0.602778 0 Td 46.180556 Tz (rich ) Tj 1.263889 0 Td 46.990741 Tz (in ) Tj 0.7 0 Td 50.636574 Tz (collagen ) Tj 2.547222 0 Td 53.472222 Tz (type ) Tj 1.438889 0 Td 37.268519 Tz (II ) Tj 0.583333 0 Td 54.282407 Tz (Such ) Tj 1.458333 0 Td 48.611111 Tz (as ) Tj 0.719444 0 Td 50.771605 Tz (the ) Tj 1.05 0 Td 47.170782 Tz (vertebral ) Tj 51.428571 0 0 51.428571 0 2169.735426 Tm 25.297222 0 Td 51.446759 Tz (endplate ) Tj 2.644444 0 Td 58.333333 Tz (and ) Tj 1.205556 0 Td 46.090535 Tz (articular ) Tj 2.625 0 Td 44.398148 Tz (cartilage. ) Tj 51.428571 0 0 51.428571 0 2126.468085 Tm 26.016667 0 Td 63.734568 Tz (The ) Tj 1.361111 0 Td 55.092593 Tz (term ) Tj 1.575 0 Td 49.259259 Tz (bioactive ) Tj 3.169444 0 Td 53.148148 Tz (agent ) Tj 2.158333 0 Td 66.975309 Tz (may ) Tj 1.438889 0 Td 51.388889 Tz (include ) Tj 2.352778 0 Td 58.333333 Tz (an ) Tj 0.933333 0 Td 52.5 Tz (agent ) Tj 1.788889 0 Td 54.552469 Tz (having ) Tj 51.428571 0 0 51.428571 0 2086.430769 Tm 25.277778 0 Td 46.843434 Tz (bioactivity ) Tj 3.286111 0 Td 54.282407 Tz (Such ) Tj 1.497222 0 Td 43.209877 Tz (as, ) Tj 0.952778 0 Td 48.611111 Tz (for ) Tj 0.991667 0 Td 55.092593 Tz (example, ) Tj 2.819444 0 Td 50.968013 Tz (angiogenic, ) Tj 3.538889 0 Td 48.611111 Tz (osteoinductive, ) Tj 51.428571 0 0 51.428571 0 2043.383908 Tm 25.297222 0 Td 50.83912 Tz (osteoconductive, ) Tj 5.036111 0 Td 53.472222 Tz (orpharmaceutical ) Tj 5.269444 0 Td 48.148148 Tz (agents. ) Tj 2.158333 0 Td 50.87963 Tz (Therefore, ) Tj 3.169444 0 Td 60.925926 Tz (bioac ) Tj 51.428571 0 0 51.428571 0 2001.857837 Tm 25.277778 0 Td 45.37037 Tz (tive ) Tj 1.263889 0 Td 51.851852 Tz (agents ) Tj 2.1 0 Td 68.055556 Tz (may ) Tj 1.458333 0 Td 50.462963 Tz (include ) Tj 2.352778 0 Td 47.530864 Tz (for ) Tj 1.030556 0 Td 54.6875 Tz (example, ) Tj 2.838889 0 Td 48.179012 Tz (antimicrobials, ) Tj 4.55 0 Td 52.391975 Tz (antibi ) Tj 51.428571 0 0 51.428571 0 1962.905855 Tm 25.297222 0 Td 43.209877 Tz (otics, ) Tj 1.691667 0 Td 49.56427 Tz (antimyobacterial, ) Tj 5.191667 0 Td 47.260802 Tz (antifungals, ) Tj 3.519444 0 Td 43.602694 Tz (antivirals, ) Tj 3.013889 0 Td 48.148148 Tz (antineoplastic ) Tj 51.428571 0 0 51.428571 0 1919.645503 Tm 25.297222 0 Td 48.611111 Tz (agents, ) Tj 2.488889 0 Td 53.292181 Tz (antitumor ) Tj 3.325 0 Td 48.611111 Tz (agents, ) Tj 2.508333 0 Td 51.851852 Tz (agents ) Tj 2.333333 0 Td 47.890947 Tz (affecting ) Tj 3.052778 0 Td 49.691358 Tz (the ) Tj 1.380556 0 Td 66.435185 Tz (immune ) Tj 51.428571 0 0 51.428571 0 1878.908213 Tm 25.297222 0 Td 50.411523 Tz (response, ) Tj 2.916667 0 Td 55.092593 Tz (blood ) Tj 1.847222 0 Td 53.703704 Tz (calcium ) Tj 2.469444 0 Td 46.548822 Tz (regulators, ) Tj 3.266667 0 Td 51.311728 Tz (agents ) Tj 2.041667 0 Td 49.151235 Tz (useful ) Tj 1.963889 0 Td 45.37037 Tz (in ) Tj 0.738889 0 Td 53.240741 Tz (glucose ) Tj 51.428571 0 0 51.428571 0 1837.652695 Tm 25.297222 0 Td 47.138047 Tz (regulation, ) Tj 3.519444 0 Td 48.82716 Tz (anticoagulants, ) Tj 4.802778 0 Td 49.623843 Tz (antithrombotics, ) Tj 5.172222 0 Td 50.854701 Tz (antihyperlipi ) Tj 51.428571 0 0 51.428571 0 1796.512852 Tm 25.297222 0 Td 58.981481 Tz (demic ) Tj 2.022222 0 Td 48.611111 Tz (agents, ) Tj 2.333333 0 Td 50 Tz (cardiac ) Tj 2.352778 0 Td 49.691358 Tz (drugs, ) Tj 2.061111 0 Td 54.282407 Tz (thyromimetic ) Tj 4.180556 0 Td 59.41358 Tz (and ) Tj 1.322222 0 Td 48.611111 Tz (antithyroid ) Tj 51.428571 0 0 51.428571 0 1757 Tm 25.297222 0 Td 49.691358 Tz (drugs, ) Tj 2.236111 0 Td 48.881173 Tz (adrenergics, ) Tj 3.966667 0 Td 50.028935 Tz (antihypertensive ) Tj 5.230556 0 Td 48.611111 Tz (agents, ) Tj 2.527778 0 Td 48.341049 Tz (cholnergics, ) Tj 51.428571 0 0 51.428571 0 1713.64988 Tm 25.297222 0 Td 47.086057 Tz (anticholinergics, ) Tj 5.113889 0 Td 51.419753 Tz (antispasmodics, ) Tj 4.938889 0 Td 47.530864 Tz (antiulcer ) Tj 2.858333 0 Td 48.148148 Tz (agents, ) Tj 2.352778 0 Td 45.775463 Tz (skeletal ) Tj 51.428571 0 0 51.428571 0 1667.066667 Tm 25.297222 0 Td 58.333333 Tz (and ) Tj 1.225 0 Td 59.41358 Tz (Smooth ) Tj 2.352778 0 Td 56.712963 Tz (muscle ) Tj 2.236111 0 Td 46.342593 Tz (relaxants, ) Tj 2.975 0 Td 50.925926 Tz (general ) Tj 2.352778 0 Td 45.694444 Tz (inhibitors ) Tj 2.936111 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 51.203704 Tz (aller ) Tj 52.857143 0 0 52.857143 0 1626.393643 Tm 24.613514 0 Td 48.348348 Tz (gic ) Tj 1.172973 0 Td 48.698699 Tz (response, ) Tj 2.913514 0 Td 47.507508 Tz (antihistamines, ) Tj 4.597297 0 Td 44.774775 Tz (local ) Tj 1.627027 0 Td 45.720721 Tz (anesthetics, ) Tj 3.594595 0 Td 46.437346 Tz (analgesics, ) Tj 51.428571 0 0 51.428571 0 1587.144876 Tm 25.297222 0 Td 48.611111 Tz (narcotic ) Tj 2.566667 0 Td 48.070988 Tz (antagonists, ) Tj 3.675 0 Td 45.619658 Tz (antitussives, ) Tj 3.830556 0 Td 50.136166 Tz (sedative-hypnotic ) Tj 5.327778 0 Td 48.611111 Tz (agents, ) Tj 51.428571 0 0 51.428571 0 1544.756757 Tm 25.297222 0 Td 48.611111 Tz (anticonvulsants, ) Tj 4.880556 0 Td 48.395062 Tz (antipsychotics, ) Tj 4.588889 0 Td 48.070988 Tz (anti-anxiety ) Tj 3.713889 0 Td 48.148148 Tz (agents, ) Tj 2.236111 0 Td 56.712963 Tz (antide ) Tj 51.428571 0 0 51.428571 0 1503.125436 Tm 25.297222 0 Td 49.826389 Tz (pressant ) Tj 2.527778 0 Td 48.611111 Tz (agents, ) Tj 2.236111 0 Td 50 Tz (anorexigenics, ) Tj 4.394444 0 Td 48.611111 Tz (non-steroidal ) Tj 3.986111 0 Td 55.34188 Tz (anti-inflamma ) Tj 51.428571 0 0 51.428571 0 1462.034965 Tm 25.277778 0 Td 50.231481 Tz (tory ) Tj 1.341667 0 Td 48.148148 Tz (agents, ) Tj 2.197222 0 Td 45.730453 Tz (steroidal ) Tj 2.625 0 Td 51.470588 Tz (anti-inflammatory ) Tj 5.405556 0 Td 48.148148 Tz (agents, ) Tj 2.177778 0 Td 47.61396 Tz (antioxidants, ) Tj 51.428571 0 0 51.428571 0 1418.590244 Tm 25.297222 0 Td 49.789562 Tz (vaso-active ) Tj 3.538889 0 Td 49.074074 Tz (agents, ) Tj 2.352778 0 Td 51.262626 Tz (bone-active ) Tj 3.675 0 Td 48.148148 Tz (agents, ) Tj 2.352778 0 Td 51.203704 Tz (osteogenic ) Tj 3.402778 0 Td 44.155093 Tz (factors, ) Tj 51.428571 0 0 51.428571 0 1377.956044 Tm 25.297222 0 Td 42.777778 Tz (antiarthritics, ) Tj 4.122222 0 Td 53.148148 Tz (drugs ) Tj 1.866667 0 Td 56.17284 Tz (can ) Tj 1.283333 0 Td 47.800926 Tz (include, ) Tj 2.566667 0 Td 49.691358 Tz (for ) Tj 1.088889 0 Td 55.497685 Tz (example, ) Tj 2.897222 0 Td 50.231481 Tz (hyaluronate, ) Tj 51.428571 0 0 51.428571 0 1334.515971 Tm 25.297222 0 Td 53.240741 Tz (Vitamin ) Tj 2.45 0 Td 53.472222 Tz (D. ) Tj 0.855556 0 Td 44.965278 Tz (Statins, ) Tj 2.372222 0 Td 52.314815 Tz (vitamin ) Tj 2.411111 0 Td 87.5 Tz (K ) Tj 0.680556 0 Td 58.333333 Tz (and ) Tj 1.263889 0 Td 51.851852 Tz (bisphosphonates, ) Tj 5.191667 0 Td 51.851852 Tz (or ) Tj 0.816667 0 Td 78.858025 Tz (mus ) Tj 51.428571 0 0 51.428571 0 1292.014493 Tm 25.297222 0 Td 57.523148 Tz (cone ) Tj 1.536111 0 Td 55.902778 Tz (\(She ) Tj 1.497222 0 Td 56.712963 Tz (Xiang\) ) Tj 2.177778 0 Td 59.41358 Tz (and ) Tj 1.244444 0 Td 38.888889 Tz (salt, ) Tj 1.380556 0 Td 48.341049 Tz (metabolites, ) Tj 3.675 0 Td 48.206019 Tz (derivate ) Tj 2.488889 0 Td 46.180556 Tz (thereof; ) Tj 2.391667 0 Td 60.493827 Tz (and ) Tj 51.428571 0 0 51.428571 0 1250.527981 Tm 25.297222 0 Td 50.87963 Tz (progenitor ) Tj 3.227778 0 Td 41.049383 Tz (cells, ) Tj 1.652778 0 Td 55.092593 Tz (such ) Tj 1.516667 0 Td 48.611111 Tz (as ) Tj 0.797222 0 Td 55.902778 Tz (stem ) Tj 1.536111 0 Td 41.589506 Tz (cells; ) Tj 1.711111 0 Td 47.222222 Tz (natural ) Tj 2.158333 0 Td 59.41358 Tz (and ) Tj 1.225 0 Td 55.387205 Tz (recombinant ) Tj 51.428571 0 0 51.428571 0 1212.973236 Tm 25.297222 0 Td 56.17284 Tz (growth ) Tj 2.255556 0 Td 44.965278 Tz (factors, ) Tj 2.352778 0 Td 59.41358 Tz (and ) Tj 1.283333 0 Td 57.253086 Tz (exogenous ) Tj 3.286111 0 Td 57.253086 Tz (growth ) Tj 2.294444 0 Td 46.759259 Tz (factors ) Tj 2.197222 0 Td 51.131687 Tz (including ) Tj 2.975 0 Td 47.530864 Tz (for ) Tj 51.428571 0 0 51.428571 0 1171.475248 Tm 25.297222 0 Td 54.282407 Tz (example: ) Tj 2.936111 0 Td 51.851852 Tz (transforming ) Tj 3.966667 0 Td 56.712963 Tz (growth ) Tj 2.333333 0 Td 47.530864 Tz (factor ) Tj 2.061111 0 Td 43.75 Tz (B- ) Tj 0.777778 0 Td 56.712963 Tz (\(TGF-B\), ) Tj 3.013889 0 Td 49.421296 Tz (platelet ) Tj 51.428571 0 0 51.428571 0 1126.097867 Tm 25.297222 0 Td 51.388889 Tz (derived ) Tj 2.469444 0 Td 56.712963 Tz (growth ) Tj 2.391667 0 Td 48.070988 Tz (factor ) Tj 2.041667 0 Td 59.259259 Tz (\(PDGF\), ) Tj 2.858333 0 Td 46.180556 Tz (insulin-like ) Tj 3.636111 0 Td 56.17284 Tz (growth ) Tj 2.372222 0 Td 48.070988 Tz (factor ) Tj 51.428571 0 0 51.428571 0 1085.458537 Tm 25.316667 0 Td 50.231481 Tz (\(IGF\), ) Tj 2.080556 0 Td 49.259259 Tz (sonic ) Tj 1.75 0 Td 59.143519 Tz (hedgehog ) Tj 3.072222 0 Td 58.873457 Tz (\(SHH\), ) Tj 2.391667 0 Td 50.231481 Tz (vascular ) Tj 2.625 0 Td 48.905724 Tz (endothelial ) Tj 3.480556 0 Td 57.253086 Tz (growth ) Tj 51.428571 0 0 51.428571 0 1044.241706 Tm 25.297222 0 Td 47.530864 Tz (factor ) Tj 1.847222 0 Td 61.111111 Tz (\(VEGF\), ) Tj 2.722222 0 Td 59.953704 Tz (bone ) Tj 1.633333 0 Td 55.34188 Tz (morphogenetic ) Tj 4.491667 0 Td 48.611111 Tz (proteins ) Tj 2.527778 0 Td 58.333333 Tz (\(BMPs\), ) Tj 2.644444 0 Td 53.148148 Tz (fibro ) Tj 51.428571 0 0 51.428571 0 1002.653892 Tm 25.277778 0 Td 46.666667 Tz (blast ) Tj 1.555556 0 Td 56.712963 Tz (growth ) Tj 2.255556 0 Td 47.530864 Tz (factor ) Tj 1.866667 0 Td 60.925926 Tz (\(FGF\) ) Tj 1.983333 0 Td 59.41358 Tz (and ) Tj 1.244444 0 Td 50.673401 Tz (parathyroid ) Tj 3.519444 0 Td 62.037037 Tz (hormone ) Tj 2.8 0 Td 53.240741 Tz (\(PTH\)\), ) Tj 40 0 0 40 0 966 Tm 32.525 0 Td 67.5 Tz (alone ) Tj 2.25 0 Td 68.75 Tz (or ) Tj 1.025 0 Td 58.333333 Tz (in ) Tj 0.975 0 Td 64.285714 Tz (various ) Tj 2.975 0 Td 66.987179 Tz (combinations. ) Tj 51.428571 0 0 51.428571 0 920.791123 Tm 26.016667 0 Td 63.734568 Tz (The ) Tj 1.341667 0 Td 55.092593 Tz (term ) Tj 1.536111 0 Td 47.863248 Tz (bioactivity ) Tj 4.005556 0 Td 60.277778 Tz (means ) Tj 2.041667 0 Td 48.611111 Tz (the ) Tj 1.069444 0 Td 45.37037 Tz (effect ) Tj 1.827778 0 Td 59.953704 Tz (of ) Tj 0.816667 0 Td 55.092593 Tz (a ) Tj 0.544444 0 Td 52.5 Tz (given ) Tj 1.788889 0 Td 48.611111 Tz (agent, ) Tj 51.428571 0 0 51.428571 0 877.54678 Tm 25.316667 0 Td 54.282407 Tz (Such ) Tj 1.438889 0 Td 50.231481 Tz (as ) Tj 0.738889 0 Td 51.851852 Tz (a ) Tj 0.486111 0 Td 48.611111 Tz (vaccine, ) Tj 2.488889 0 Td 56.271044 Tz (medicament, ) Tj 3.869444 0 Td 51.851852 Tz (or ) Tj 0.719444 0 Td 56.17284 Tz (growth ) Tj 2.197222 0 Td 46.990741 Tz (factor ) Tj 1.808333 0 Td 59.143519 Tz (upon ) Tj 1.575 0 Td 55.092593 Tz (a ) Tj 0.466667 0 Td 45.910494 Tz (living ) Tj 51.428571 0 0 51.428571 0 837.293578 Tm 25.297222 0 Td 52.932099 Tz (organism, ) Tj 3.052778 0 Td 46.990741 Tz (living ) Tj 1.847222 0 Td 43.981481 Tz (tissue, ) Tj 2.061111 0 Td 50.231481 Tz (or ) Tj 0.777778 0 Td 41.049383 Tz (cells. ) Tj 51.428571 0 0 51.428571 0 795.698595 Tm 26.016667 0 Td 63.734568 Tz (The ) Tj 1.322222 0 Td 53.472222 Tz (term ) Tj 1.477778 0 Td 47.193287 Tz (fibrocartilage' ) Tj 4.725 0 Td 60.925926 Tz (means ) Tj 2.022222 0 Td 51.851852 Tz (a ) Tj 0.505556 0 Td 52.662037 Tz (kind ) Tj 1.438889 0 Td 58.333333 Tz (of ) Tj 0.797222 0 Td 46.450617 Tz (cartilage ) Tj 2.663889 0 Td 54.282407 Tz (with ) Tj 1.458333 0 Td 58.333333 Tz (a ) Tj 51.428571 0 0 51.428571 0 751.821297 Tm 25.297222 0 Td 47.685185 Tz (fibrous ) Tj 2.255556 0 Td 52.391975 Tz (matrix ) Tj 2.080556 0 Td 59.41358 Tz (and ) Tj 1.283333 0 Td 54.79798 Tz (approaching ) Tj 3.85 0 Td 47.222222 Tz (fibrous ) Tj 2.216667 0 Td 52.175926 Tz (connective ) Tj 3.344444 0 Td 45.37037 Tz (tissue ) Tj 1.886111 0 Td 46.990741 Tz (in ) Tj 34.285714 0 0 34.285714 0 717 Tm 37.975 0 Td 67.083333 Tz (Structure. ) Tj 51.428571 0 0 51.428571 0 669.726368 Tm 26.016667 0 Td 63.734568 Tz (The ) Tj 1.341667 0 Td 54.282407 Tz (term ) Tj 1.477778 0 Td 49.331276 Tz (osteoinductivity ) Tj 5.541667 0 Td 60.925926 Tz (means ) Tj 2.002778 0 Td 49.151235 Tz (acceleration ) Tj 3.713889 0 Td 55.092593 Tz (or ) Tj 0.836111 0 Td 60.925926 Tz (induc ) Tj 51.428571 0 0 51.428571 0 626.969697 Tm 25.277778 0 Td 46.990741 Tz (tion ) Tj 1.322222 0 Td 58.333333 Tz (of ) Tj 0.797222 0 Td 63.734568 Tz (new ) Tj 1.322222 0 Td 59.953704 Tz (bone ) Tj 1.594444 0 Td 52.932099 Tz (formation ) Tj 3.052778 0 Td 59.953704 Tz (by ) Tj 0.875 0 Td 54.6875 Tz (chemical ) Tj 2.8 0 Td 53.472222 Tz (or ) Tj 0.777778 0 Td 53.914141 Tz (biochemical ) Tj 3.713889 0 Td 68.055556 Tz (pro ) Tj 51.428571 0 0 51.428571 0 586.523297 Tm 25.297222 0 Td 45.37037 Tz (cess. ) Tj 1.497222 0 Td 87.5 Tz (A ) Tj 0.680556 0 Td 69.675926 Tz (common ) Tj 2.663889 0 Td 42.939815 Tz (test ) Tj 1.147222 0 Td 48.611111 Tz (for ) Tj 0.991667 0 Td 50.326797 Tz (osteoconductivity ) Tj 5.288889 0 Td 40.509259 Tz (is ) Tj 0.641667 0 Td 49.691358 Tz (the ) Tj 0.991667 0 Td 61.574074 Tz (bone ) Tj 1.575 0 Td 66.111111 Tz (forma ) Tj 51.428571 0 0 51.428571 0 544.597403 Tm 25.277778 0 Td 46.990741 Tz (tion ) Tj 1.361111 0 Td 43.75 Tz (in ) Tj 0.7 0 Td 58.333333 Tz (a ) Tj 0.544444 0 Td 56.712963 Tz (muscle ) Tj 2.216667 0 Td 54.012346 Tz (pouch. ) Tj 51.428571 0 0 51.428571 0 504.608247 Tm 26.016667 0 Td 63.734568 Tz (The ) Tj 1.361111 0 Td 55.092593 Tz (term ) Tj 1.555556 0 Td 50.82846 Tz (osteoconductivity ) Tj 6.047222 0 Td 45.37037 Tz (refers ) Tj 1.886111 0 Td 43.75 Tz (to ) Tj 0.777778 0 Td 56.17284 Tz (any ) Tj 1.283333 0 Td 47.170782 Tz (structure ) Tj 2.741667 0 Td 46.180556 Tz (that ) Tj 51.428571 0 0 51.428571 0 460.577332 Tm 25.297222 0 Td 42.718855 Tz (facilitates ) Tj 2.936111 0 Td 49.691358 Tz (the ) Tj 1.011111 0 Td 52.932099 Tz (formation ) Tj 2.994444 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 58.333333 Tz (bone ) Tj 1.536111 0 Td 44.722222 Tz (structure. ) Tj 2.838889 0 Td 67.650463 Tz (Commonly ) Tj 3.383333 0 Td 55.902778 Tz (used ) Tj 1.438889 0 Td 46.990741 Tz (to ) Tj 51.428571 0 0 51.428571 0 421.145386 Tm 25.297222 0 Td 50.636574 Tz (describe ) Tj 2.663889 0 Td 50.771605 Tz (the ) Tj 1.147222 0 Td 47.962963 Tz (properties ) Tj 3.169444 0 Td 56.712963 Tz (of ) Tj 0.875 0 Td 49.537037 Tz (various ) Tj 2.333333 0 Td 51.203704 Tz (types ) Tj 1.788889 0 Td 58.333333 Tz (of ) Tj 0.836111 0 Td 60.763889 Tz (bone ) Tj 1.691667 0 Td 45.37037 Tz (grafts ) Tj 1.905556 0 Td 59.41358 Tz (and ) Tj 51.428571 0 0 51.428571 0 377.832258 Tm 25.277778 0 Td 59.953704 Tz (bone ) Tj 1.613889 0 Td 46.018519 Tz (graft ) Tj 1.575 0 Td 44.560185 Tz (Substitutes. ) Tj 51.428571 0 0 51.428571 0 337.869677 Tm 26.016667 0 Td 63.734568 Tz (The ) Tj 1.322222 0 Td 53.472222 Tz (term ) Tj 1.458333 0 Td 52.391975 Tz (osteogenic ) Tj 3.947222 0 Td 45.37037 Tz (refers ) Tj 1.808333 0 Td 45.37037 Tz (to ) Tj 0.719444 0 Td 54.444444 Tz (being ) Tj 1.808333 0 Td 46.759259 Tz (related ) Tj 2.138889 0 Td 45.37037 Tz (to ) Tj 0.7 0 Td 59.953704 Tz (bone ) Tj 1.575 0 Td 58.333333 Tz (be ) Tj 0.875 0 Td 34.027778 Tz (it ) Tj 51.428571 0 0 51.428571 0 295.83905 Tm 25.297222 0 Td 56.17284 Tz (growth ) Tj 2.236111 0 Td 44.560185 Tz (factors, ) Tj 2.352778 0 Td 42.12963 Tz (cell ) Tj 1.205556 0 Td 53.472222 Tz (phenotype, ) Tj 3.383333 0 Td 53.472222 Tz (or ) Tj 0.777778 0 Td 47.115385 Tz (extracellular ) Tj 3.85 0 Td 48.611111 Tz (matrix. ) Tj 51.428571 0 0 51.428571 0 253.867347 Tm 26.016667 0 Td 63.734568 Tz (The ) Tj 1.380556 0 Td 54.282407 Tz (term ) Tj 1.575 0 Td 51.851852 Tz (osteogenic ) Tj 3.675 0 Td 46.383102 Tz (differentiation' ) Tj 4.725 0 Td 60.925926 Tz (means ) Tj 2.061111 0 Td 43.75 Tz (cell ) Tj 1.283333 0 Td 68.055556 Tz (pheno ) Tj 51.428571 0 0 51.428571 0 214.271106 Tm 25.277778 0 Td 51.203704 Tz (types ) Tj 1.75 0 Td 48.935185 Tz (consistent ) Tj 3.130556 0 Td 56.712963 Tz (of ) Tj 0.777778 0 Td 55.902778 Tz (bone-forming ) Tj 4.238889 0 Td 46.990741 Tz (\(osteoblast\) ) Tj 3.616667 0 Td 51.851852 Tz (or ) Tj 0.797222 0 Td 58.693416 Tz (bone-regu ) Tj 51.428571 0 0 51.428571 0 174.148148 Tm 25.297222 0 Td 45.910494 Tz (lating ) Tj 1.866667 0 Td 48.316498 Tz (\(osteocyte\) ) Tj 3.402778 0 Td 40.509259 Tz (cells. ) Tj ET Q endstream endobj 458 0 obj << /Length 1 >> stream q endstream endobj 459 0 obj << /Length 47794 >> stream Q q 0.24 0 0 0.24 0 0 cm BT /F1 1 Tf 3 Tr 62.857143 0 0 62.857143 0 3023 Tm 17.325 0 Td 83.522727 Tz (US ) Tj 1.240909 0 Td 58.333333 Tz (8,404,256 ) Tj 3.388636 0 Td 74.242424 Tz (B2 ) Tj 51.428571 0 0 51.428571 0 2915.711514 Tm 7.097222 0 Td 62.654321 Tz (The ) Tj 1.283333 0 Td 54.282407 Tz (term ) Tj 1.458333 0 Td 46.342593 Tz (platelet' ) Tj 2.936111 0 Td 40.509259 Tz (is ) Tj 0.641667 0 Td 55.092593 Tz (used ) Tj 1.477778 0 Td 51.851852 Tz (here ) Tj 1.419444 0 Td 45.37037 Tz (to ) Tj 0.7 0 Td 46.018519 Tz (refer ) Tj 1.516667 0 Td 45.37037 Tz (to ) Tj 0.680556 0 Td 51.851852 Tz (a ) Tj 0.486111 0 Td 55.740741 Tz (blood ) Tj 1.788889 0 Td 42.849794 Tz (platelet. ) Tj 51.428571 0 0 51.428571 0 2874.962606 Tm 6.358333 0 Td 87.5 Tz (A ) Tj 0.661111 0 Td 45.37037 Tz (platelet ) Tj 2.333333 0 Td 54.012346 Tz (can ) Tj 1.127778 0 Td 61.574074 Tz (be ) Tj 0.875 0 Td 51.49177 Tz (described ) Tj 2.916667 0 Td 51.851852 Tz (as ) Tj 0.758333 0 Td 55.092593 Tz (a ) Tj 0.525 0 Td 54.73251 Tz (minuscule ) Tj 3.091667 0 Td 52.662037 Tz (protoplasmic ) Tj 3.986111 0 Td 51.041667 Tz (disk ) Tj 51.428571 0 0 51.428571 0 2832.098361 Tm 6.358333 0 Td 51.851852 Tz (occurring ) Tj 3.033333 0 Td 43.75 Tz (in ) Tj 0.797222 0 Td 47.962963 Tz (vertebrate ) Tj 3.072222 0 Td 50.771605 Tz (blood. ) Tj 2.080556 0 Td 45.37037 Tz (Platelets ) Tj 2.663889 0 Td 51.851852 Tz (play ) Tj 1.458333 0 Td 55.092593 Tz (a ) Tj 0.583333 0 Td 46.180556 Tz (role ) Tj 1.341667 0 Td 45.37037 Tz (in ) Tj 0.758333 0 Td 57.037037 Tz (blood ) Tj 51.428571 0 0 51.428571 0 2791.376058 Tm 6.358333 0 Td 43.930041 Tz (clotting. ) Tj 2.547222 0 Td 62.654321 Tz (The ) Tj 1.263889 0 Td 44.965278 Tz (platelet ) Tj 2.313889 0 Td 66.975309 Tz (may ) Tj 1.361111 0 Td 58.333333 Tz (be ) Tj 0.836111 0 Td 51.388889 Tz (derived ) Tj 2.313889 0 Td 57.523148 Tz (from ) Tj 1.516667 0 Td 58.333333 Tz (any ) Tj 1.225 0 Td 52.391975 Tz (source ) Tj 2.041667 0 Td 56.712963 Tz (includ ) Tj 51.428571 0 0 51.428571 0 2749.704626 Tm 6.358333 0 Td 52.932099 Tz (ing ) Tj 1.030556 0 Td 58.333333 Tz (a ) Tj 0.447222 0 Td 66.111111 Tz (human ) Tj 2.119444 0 Td 53.472222 Tz (or ) Tj 0.758333 0 Td 63.374486 Tz (mammalian ) Tj 3.538889 0 Td 55.092593 Tz (blood ) Tj 1.769444 0 Td 49.537037 Tz (Supply, ) Tj 2.216667 0 Td 51.851852 Tz (or ) Tj 0.7 0 Td 50.771605 Tz (the ) Tj 1.011111 0 Td 52.256944 Tz (patients ) Tj 2.644444 0 Td 70.216049 Tz (own ) Tj 51.428571 0 0 51.428571 0 2707.940334 Tm 6.338889 0 Td 51.311728 Tz (blood. ) Tj 1.983333 0 Td 53.796296 Tz (Thus, ) Tj 1.75 0 Td 49.691358 Tz (the ) Tj 1.030556 0 Td 44.650206 Tz (platelets ) Tj 2.566667 0 Td 42.12963 Tz (in ) Tj 0.680556 0 Td 49.691358 Tz (the ) Tj 0.991667 0 Td 54.208754 Tz (composition ) Tj 3.733333 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 48.611111 Tz (the ) Tj 0.991667 0 Td 49.907407 Tz (inventions ) Tj 51.428571 0 0 51.428571 0 2666.24235 Tm 6.358333 0 Td 69.135802 Tz (may ) Tj 1.4 0 Td 58.333333 Tz (be ) Tj 0.816667 0 Td 53.472222 Tz (autologous ) Tj 3.383333 0 Td 35.648148 Tz (i.e. ) Tj 1.05 0 Td 56.712963 Tz (from ) Tj 1.536111 0 Td 49.691358 Tz (the ) Tj 1.069444 0 Td 61.574074 Tz (same ) Tj 1.613889 0 Td 65.462963 Tz (human ) Tj 2.119444 0 Td 47.685185 Tz (treated ) Tj 2.138889 0 Td 53.472222 Tz (with ) Tj 1.458333 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 2625.15092 Tm 6.358333 0 Td 54.208754 Tz (composition ) Tj 3.772222 0 Td 51.851852 Tz (or ) Tj 0.738889 0 Td 49.331276 Tz (allogenic ) Tj 2.858333 0 Td 34.837963 Tz (i.e. ) Tj 1.05 0 Td 56.712963 Tz (from ) Tj 1.516667 0 Td 58.333333 Tz (a ) Tj 0.486111 0 Td 66.111111 Tz (human ) Tj 2.138889 0 Td 46.180556 Tz (but, ) Tj 1.302778 0 Td 50.771605 Tz (not ) Tj 1.05 0 Td 50.771605 Tz (the ) Tj 1.05 0 Td 63.194444 Tz (same ) Tj 51.428571 0 0 51.428571 0 2584.743243 Tm 6.338889 0 Td 65.462963 Tz (human ) Tj 2.177778 0 Td 55.092593 Tz (being ) Tj 1.808333 0 Td 47.222222 Tz (treated ) Tj 2.177778 0 Td 52.662037 Tz (with ) Tj 1.438889 0 Td 50.771605 Tz (the ) Tj 1.108333 0 Td 51.851852 Tz (composition. ) Tj 51.428571 0 0 51.428571 0 2542.549738 Tm 7.097222 0 Td 61.574074 Tz (The ) Tj 1.380556 0 Td 55.092593 Tz (term ) Tj 1.594444 0 Td 47.7657 Tz (platelet-rich-plasma, ) Tj 6.863889 0 Td 64.166667 Tz (PRP ) Tj 2.197222 0 Td 58.333333 Tz (and ) Tj 1.322222 0 Td 48.611111 Tz (the ) Tj 1.147222 0 Td 44.560185 Tz (like ) Tj 1.322222 0 Td 50.771605 Tz (are ) Tj 51.428571 0 0 51.428571 0 2500.149144 Tm 6.338889 0 Td 56.712963 Tz (used ) Tj 1.555556 0 Td 51.203704 Tz (interchangeable ) Tj 4.802778 0 Td 52.662037 Tz (here ) Tj 1.438889 0 Td 46.990741 Tz (to ) Tj 0.797222 0 Td 64.00463 Tz (mean ) Tj 1.75 0 Td 55.092593 Tz (a ) Tj 0.544444 0 Td 50.356125 Tz (concentration ) Tj 4.161111 0 Td 55.092593 Tz (of ) Tj 0.777778 0 Td 55.092593 Tz (plate ) Tj 51.428571 0 0 51.428571 0 2457.222494 Tm 6.358333 0 Td 42.12963 Tz (lets ) Tj 1.186111 0 Td 45.37037 Tz (in ) Tj 0.7 0 Td 55.092593 Tz (a ) Tj 0.505556 0 Td 45.833333 Tz (carrier ) Tj 2.061111 0 Td 58.333333 Tz (which ) Tj 1.925 0 Td 50.605413 Tz (concentration ) Tj 4.141667 0 Td 38.888889 Tz (is ) Tj 0.622222 0 Td 57.685185 Tz (above ) Tj 1.905556 0 Td 45.37037 Tz (that ) Tj 1.225 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 45.010288 Tz (platelets ) Tj 51.428571 0 0 51.428571 0 2417.790524 Tm 6.358333 0 Td 53.877315 Tz (normally ) Tj 2.877778 0 Td 55.740741 Tz (found ) Tj 1.905556 0 Td 45.37037 Tz (in ) Tj 0.777778 0 Td 50.231481 Tz (blood. ) Tj 2.1 0 Td 55.092593 Tz (For ) Tj 1.147222 0 Td 55.092593 Tz (example, ) Tj 2.877778 0 Td 48.611111 Tz (the ) Tj 1.088889 0 Td 44.965278 Tz (platelet ) Tj 2.372222 0 Td 64.274691 Tz (concen ) Tj 51.428571 0 0 51.428571 0 2373.691771 Tm 6.338889 0 Td 45.37037 Tz (tration ) Tj 2.255556 0 Td 66.975309 Tz (may ) Tj 1.516667 0 Td 58.333333 Tz (be ) Tj 1.030556 0 Td 55.092593 Tz (5 ) Tj 0.661111 0 Td 47.530864 Tz (times, ) Tj 2.1 0 Td 58.333333 Tz (10 ) Tj 0.972222 0 Td 48.611111 Tz (times, ) Tj 2.158333 0 Td 57.253086 Tz (100 ) Tj 1.302778 0 Td 52.5 Tz (times ) Tj 1.866667 0 Td 55.092593 Tz (or ) Tj 0.952778 0 Td 61.574074 Tz (more ) Tj 1.788889 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 2332.810427 Tm 6.358333 0 Td 57.253086 Tz (normal ) Tj 2.158333 0 Td 51.103989 Tz (concentration ) Tj 4.083333 0 Td 48.611111 Tz (in ) Tj 0.661111 0 Td 50.771605 Tz (blood. ) Tj 1.963889 0 Td 62.654321 Tz (The ) Tj 1.283333 0 Td 70.216049 Tz (PRP ) Tj 1.4 0 Td 66.975309 Tz (may ) Tj 1.341667 0 Td 54.012346 Tz (use ) Tj 1.088889 0 Td 48.611111 Tz (the ) Tj 0.972222 0 Td 52.256944 Tz (patients ) Tj 51.428571 0 0 51.428571 0 2293.960494 Tm 6.358333 0 Td 68.055556 Tz (own ) Tj 1.4 0 Td 57.253086 Tz (plasma ) Tj 2.197222 0 Td 51.851852 Tz (as ) Tj 0.777778 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 45.833333 Tz (carrier ) Tj 2.041667 0 Td 60.493827 Tz (and ) Tj 1.244444 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 44.650206 Tz (platelets ) Tj 2.625 0 Td 66.975309 Tz (may ) Tj 1.380556 0 Td 58.333333 Tz (be ) Tj 0.836111 0 Td 50.462963 Tz (present ) Tj 2.294444 0 Td 48.611111 Tz (in ) Tj 51.428571 0 0 51.428571 0 2249.884712 Tm 6.338889 0 Td 50.771605 Tz (the ) Tj 1.05 0 Td 57.79321 Tz (plasma ) Tj 2.236111 0 Td 45.37037 Tz (at ) Tj 0.7 0 Td 51.851852 Tz (a ) Tj 0.525 0 Td 52.5 Tz (range ) Tj 1.75 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 57.523148 Tz (from ) Tj 1.555556 0 Td 53.796296 Tz (about ) Tj 1.788889 0 Td 56.944444 Tz (200,000 ) Tj 2.527778 0 Td 51.851852 Tz (or ) Tj 0.777778 0 Td 44.560185 Tz (less ) Tj 1.263889 0 Td 45.37037 Tz (to ) Tj 0.738889 0 Td 49.151235 Tz (2,000, ) Tj 51.428571 0 0 51.428571 0 2208.337855 Tm 6.358333 0 Td 62.654321 Tz (000 ) Tj 1.244444 0 Td 55.092593 Tz (or ) Tj 0.855556 0 Td 60.763889 Tz (more ) Tj 1.613889 0 Td 45.010288 Tz (platelets ) Tj 2.605556 0 Td 54.012346 Tz (per ) Tj 1.088889 0 Td 53.148148 Tz (cubic ) Tj 1.788889 0 Td 48.316498 Tz (centimeter. ) Tj 3.363889 0 Td 62.654321 Tz (The ) Tj 1.341667 0 Td 71.296296 Tz (PRP ) Tj 1.477778 0 Td 66.975309 Tz (may ) Tj 1.380556 0 Td 59.953704 Tz (be ) Tj 51.428571 0 0 51.428571 0 2167.572152 Tm 6.358333 0 Td 58.333333 Tz (formed ) Tj 2.352778 0 Td 56.712963 Tz (from ) Tj 1.672222 0 Td 58.333333 Tz (whole ) Tj 2.002778 0 Td 55.740741 Tz (blood ) Tj 1.905556 0 Td 42.12963 Tz (e.g. ) Tj 1.283333 0 Td 59.953704 Tz (by ) Tj 0.991667 0 Td 53.472222 Tz (technology ) Tj 3.480556 0 Td 49.691358 Tz (disclosed ) Tj 2.955556 0 Td 47.530864 Tz (for ) Tj 51.428571 0 0 51.428571 0 2125.07771 Tm 6.358333 0 Td 58.796296 Tz (example ) Tj 2.644444 0 Td 46.990741 Tz (in ) Tj 0.758333 0 Td 52.662037 Tz (U.S. ) Tj 1.458333 0 Td 45.37037 Tz (Pat. ) Tj 1.283333 0 Td 59.41358 Tz (No. ) Tj 1.263889 0 Td 50.87963 Tz (5,614,106. ) Tj 51.428571 0 0 51.428571 0 2083.321101 Tm 7.097222 0 Td 61.574074 Tz (The ) Tj 1.341667 0 Td 53.472222 Tz (term ) Tj 1.516667 0 Td 45.37037 Tz (platelet ) Tj 2.683333 0 Td 47.314815 Tz (releasate ) Tj 3.052778 0 Td 37.268519 Tz (is ) Tj 0.680556 0 Td 49.691358 Tz (the ) Tj 1.088889 0 Td 72.376543 Tz (PRP ) Tj 1.477778 0 Td 51.851852 Tz (as ) Tj 0.836111 0 Td 50.925926 Tz (defined ) Tj 2.313889 0 Td 58.333333 Tz (above ) Tj 51.428571 0 0 51.428571 0 2041.063473 Tm 6.338889 0 Td 52.932099 Tz (but ) Tj 1.05 0 Td 47.685185 Tz (treated ) Tj 2.1 0 Td 53.472222 Tz (so ) Tj 0.777778 0 Td 46.180556 Tz (that ) Tj 1.225 0 Td 58.333333 Tz (what ) Tj 1.536111 0 Td 38.888889 Tz (is ) Tj 0.583333 0 Td 48.611111 Tz (inside ) Tj 1.866667 0 Td 49.691358 Tz (the ) Tj 1.011111 0 Td 44.965278 Tz (platelet ) Tj 2.275 0 Td 45.37037 Tz (shells ) Tj 1.769444 0 Td 49.691358 Tz (are ) Tj 0.991667 0 Td 55.555556 Tz (allowed ) Tj 51.428571 0 0 51.428571 0 2002.723785 Tm 6.338889 0 Td 46.990741 Tz (to ) Tj 0.855556 0 Td 64.814815 Tz (come ) Tj 1.808333 0 Td 44.560185 Tz (out. ) Tj 1.322222 0 Td 62.654321 Tz (The ) Tj 1.4 0 Td 46.8107 Tz (releasate ) Tj 2.8 0 Td 66.975309 Tz (may ) Tj 1.438889 0 Td 59.953704 Tz (be ) Tj 0.972222 0 Td 50.411523 Tz (subjected ) Tj 2.955556 0 Td 46.990741 Tz (to ) Tj 0.797222 0 Td 52.5 Tz (processing ) Tj 51.428571 0 0 51.428571 0 1960.96962 Tm 6.338889 0 Td 59.722222 Tz (whereby ) Tj 2.838889 0 Td 48.611111 Tz (the ) Tj 1.205556 0 Td 44.560185 Tz (platelet ) Tj 2.430556 0 Td 55.092593 Tz (cytoplasm ) Tj 3.286111 0 Td 50.771605 Tz (are ) Tj 1.244444 0 Td 59.722222 Tz (removed ) Tj 2.819444 0 Td 52.391975 Tz (and/or ) Tj 2.138889 0 Td 51.851852 Tz (other ) Tj 51.428571 0 0 51.428571 0 1917.228571 Tm 6.338889 0 Td 56.388889 Tz (blood ) Tj 1.827778 0 Td 58.333333 Tz (components ) Tj 3.636111 0 Td 50.771605 Tz (are ) Tj 1.069444 0 Td 55.902778 Tz (removed, ) Tj 2.819444 0 Td 43.75 Tz (e.g. ) Tj 1.244444 0 Td 53.148148 Tz (white ) Tj 1.730556 0 Td 55.740741 Tz (blood ) Tj 1.827778 0 Td 44.074074 Tz (cells ) Tj 1.458333 0 Td 52.932099 Tz (and/or ) Tj 51.428571 0 0 51.428571 0 1876.706027 Tm 6.358333 0 Td 51.851852 Tz (red ) Tj 1.108333 0 Td 54.444444 Tz (blood ) Tj 1.827778 0 Td 41.589506 Tz (cells, ) Tj 1.691667 0 Td 43.209877 Tz (fibrin ) Tj 1.730556 0 Td 42.777778 Tz (clot, ) Tj 1.458333 0 Td 52.391975 Tz (and/or ) Tj 2.061111 0 Td 54.012346 Tz (remaining ) Tj 3.111111 0 Td 46.450617 Tz (\(or ) Tj 1.011111 0 Td 50.636574 Tz (majority ) Tj 2.625 0 Td 46.450617 Tz (of\) ) Tj 51.428571 0 0 51.428571 0 1835.274559 Tm 6.358333 0 Td 56.712963 Tz (plasma ) Tj 2.275 0 Td 38.888889 Tz (is ) Tj 0.7 0 Td 55.497685 Tz (removed. ) Tj 2.897222 0 Td 62.654321 Tz (The ) Tj 1.322222 0 Td 74.537037 Tz (pH ) Tj 1.108333 0 Td 59.953704 Tz (of ) Tj 0.816667 0 Td 49.691358 Tz (the ) Tj 1.069444 0 Td 45.37037 Tz (platelet ) Tj 2.391667 0 Td 47.170782 Tz (releasate ) Tj 2.780556 0 Td 66.975309 Tz (may ) Tj 1.419444 0 Td 58.333333 Tz (be ) Tj 51.428571 0 0 51.428571 0 1795.534954 Tm 6.358333 0 Td 50.636574 Tz (adjusted ) Tj 2.586111 0 Td 48.611111 Tz (to ) Tj 0.758333 0 Td 50.356125 Tz (physiological ) Tj 4.102778 0 Td 74.537037 Tz (pH ) Tj 1.088889 0 Td 51.851852 Tz (or ) Tj 0.777778 0 Td 50.555556 Tz (other ) Tj 1.652778 0 Td 51.851852 Tz (as ) Tj 0.836111 0 Td 52.314815 Tz (needed. ) Tj 51.428571 0 0 51.428571 0 1750.29 Tm 7.097222 0 Td 62.654321 Tz (The ) Tj 1.302778 0 Td 53.472222 Tz (term ) Tj 1.477778 0 Td 53.742284 Tz (subchondral ) Tj 4.005556 0 Td 58.333333 Tz (bone' ) Tj 1.963889 0 Td 60.277778 Tz (means ) Tj 1.963889 0 Td 59.953704 Tz (bone ) Tj 1.575 0 Td 58.981481 Tz (which ) Tj 1.925 0 Td 52.256944 Tz (provides ) Tj 51.428571 0 0 51.428571 0 1711.418886 Tm 6.377778 0 Td 51.851852 Tz (Support ) Tj 2.333333 0 Td 48.611111 Tz (for ) Tj 0.972222 0 Td 51.851852 Tz (the ) Tj 1.05 0 Td 51.851852 Tz (hyaline ) Tj 2.333333 0 Td 46.450617 Tz (cartilage ) Tj 2.663889 0 Td 58.333333 Tz (on ) Tj 0.855556 0 Td 50.771605 Tz (the ) Tj 1.05 0 Td 45.730453 Tz (articular ) Tj 2.605556 0 Td 50 Tz (Surface ) Tj 2.275 0 Td 51.851852 Tz (or ) Tj 0.758333 0 Td 48.611111 Tz (in ) Tj 51.428571 0 0 51.428571 0 1667.490539 Tm 6.338889 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 51.851852 Tz (endplate ) Tj 2.644444 0 Td 60.493827 Tz (and ) Tj 1.263889 0 Td 53.703704 Tz (annulus ) Tj 2.430556 0 Td 50.106838 Tz (fibrosus/bone ) Tj 4.102778 0 Td 45.046296 Tz (interface. ) Tj 52.857143 0 0 52.857143 0 1625.463918 Tm 6.905405 0 Td 88.288288 Tz (A ) Tj 0.794595 0 Td 47.100225 Tz (therapeutically ) Tj 4.805405 0 Td 45.405405 Tz (effective ) Tj 3.027027 0 Td 59.384384 Tz (amount ) Tj 2.402703 0 Td 56.756757 Tz (of ) Tj 0.851351 0 Td 48.348348 Tz (the ) Tj 1.172973 0 Td 48.730549 Tz (biomaterial ) Tj 51.428571 0 0 51.428571 0 1585.257831 Tm 6.358333 0 Td 55.452675 Tz (composite ) Tj 3.188889 0 Td 54.208754 Tz (composition ) Tj 3.772222 0 Td 38.888889 Tz (is ) Tj 0.641667 0 Td 58.333333 Tz (an ) Tj 0.894444 0 Td 46.450617 Tz (effective ) Tj 2.663889 0 Td 61.574074 Tz (amount ) Tj 2.391667 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 69.351852 Tz (bioma ) Tj 51.428571 0 0 51.428571 0 1544.765957 Tm 6.338889 0 Td 42.12963 Tz (terial ) Tj 1.672222 0 Td 55.092593 Tz (composite ) Tj 3.130556 0 Td 54.208754 Tz (composition ) Tj 3.713889 0 Td 46.990741 Tz (to ) Tj 0.738889 0 Td 48.971193 Tz (stimulate ) Tj 2.761111 0 Td 45.100309 Tz (bioactivity, ) Tj 3.383333 0 Td 55.092593 Tz (such ) Tj 1.477778 0 Td 50.231481 Tz (as ) Tj 51.428571 0 0 51.428571 0 1502.943396 Tm 6.358333 0 Td 49.691358 Tz (for ) Tj 0.991667 0 Td 58.796296 Tz (example ) Tj 2.586111 0 Td 46.585648 Tz (cellular ) Tj 2.372222 0 Td 51.851852 Tz (responses ) Tj 2.975 0 Td 50.05144 Tz (inducible ) Tj 2.838889 0 Td 58.333333 Tz (by ) Tj 0.855556 0 Td 44.965278 Tz (platelet ) Tj 2.333333 0 Td 46.8107 Tz (releasate ) Tj 38.571429 0 0 38.571429 0 1463 Tm 8.477778 0 Td 68.655693 Tz (exposure. ) Tj 51.428571 0 0 51.428571 0 1418.403206 Tm 7.116667 0 Td 46.990741 Tz (In ) Tj 0.719444 0 Td 58.333333 Tz (one ) Tj 1.186111 0 Td 57.744108 Tz (embodiment, ) Tj 3.966667 0 Td 55.092593 Tz (a ) Tj 0.466667 0 Td 50.084175 Tz (biomaterial ) Tj 3.480556 0 Td 55.092593 Tz (composite ) Tj 3.130556 0 Td 51.851852 Tz (composition, ) Tj 51.428571 0 0 51.428571 0 1377.220645 Tm 6.358333 0 Td 48.206019 Tz (referred ) Tj 2.488889 0 Td 45.37037 Tz (to ) Tj 0.738889 0 Td 50.231481 Tz (as ) Tj 0.777778 0 Td 56.860269 Tz (Composition ) Tj 4.005556 0 Td 38.888889 Tz (1 ) Tj 0.486111 0 Td 38.888889 Tz (is ) Tj 0.7 0 Td 64.814815 Tz (made ) Tj 1.691667 0 Td 45.37037 Tz (of: ) Tj 1.030556 0 Td 55.092593 Tz (2 ) Tj 0.525 0 Td 61.574074 Tz (wt ) Tj 0.913889 0 Td 97.222222 Tz (% ) Tj 0.777778 0 Td 48.206019 Tz (alginate ) Tj 2.508333 0 Td 45.37037 Tz (\(as ) Tj 51.428571 0 0 51.428571 0 1334.236025 Tm 6.377778 0 Td 58.333333 Tz (sodium ) Tj 2.333333 0 Td 45.694444 Tz (alginate\); ) Tj 2.975 0 Td 63.194444 Tz (20 ) Tj 0.972222 0 Td 57.037037 Tz (vol%. ) Tj 2.022222 0 Td 56.481481 Tz (125-150 ) Tj 2.605556 0 Td 72.916667 Tz (um ) Tj 1.225 0 Td 48.070988 Tz (B-tricalcium ) Tj 3.675 0 Td 68.055556 Tz (phos ) Tj 51.428571 0 0 51.428571 0 1295.443373 Tm 6.358333 0 Td 53.148148 Tz (phate ) Tj 1.711111 0 Td 49.331276 Tz (granules; ) Tj 2.858333 0 Td 58.333333 Tz (50 ) Tj 0.875 0 Td 67.24537 Tz (vol% ) Tj 1.788889 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 48.611111 Tz (gel ) Tj 1.030556 0 Td 55.740741 Tz (phase ) Tj 1.827778 0 Td 37.268519 Tz (is ) Tj 0.602778 0 Td 51.851852 Tz (concentrated ) Tj 3.869444 0 Td 44.965278 Tz (platelet ) Tj 51.428571 0 0 51.428571 0 1251.784876 Tm 6.358333 0 Td 45.37037 Tz (releasate, ) Tj 3.188889 0 Td 55.632716 Tz (0.0100 ) Tj 2.391667 0 Td 54.166667 Tz (calcium ) Tj 2.722222 0 Td 45.833333 Tz (sulfate ) Tj 2.352778 0 Td 44.290123 Tz (\(as ) Tj 1.263889 0 Td 49.583333 Tz (dihydrate\) ) Tj 3.363889 0 Td 61.574074 Tz (powder ) Tj 51.428571 0 0 51.428571 0 1210.332506 Tm 6.338889 0 Td 56.17284 Tz (weight ) Tj 2.197222 0 Td 45.37037 Tz (to ) Tj 0.758333 0 Td 48.611111 Tz (gel ) Tj 1.088889 0 Td 55.740741 Tz (phase ) Tj 1.866667 0 Td 59.953704 Tz (Volume ) Tj 2.352778 0 Td 41.049383 Tz (ratio. ) Tj 1.75 0 Td 45.37037 Tz (In ) Tj 0.738889 0 Td 53.240741 Tz (another ) Tj 2.391667 0 Td 57.744108 Tz (embodiment, ) Tj 4.025 0 Td 55.092593 Tz (a ) Tj 51.428571 0 0 51.428571 0 1170.769231 Tm 6.338889 0 Td 50.378788 Tz (biomaterial ) Tj 3.558333 0 Td 55.452675 Tz (composite ) Tj 3.208333 0 Td 52.391975 Tz (composition, ) Tj 4.025 0 Td 47.395833 Tz (referred ) Tj 2.469444 0 Td 48.611111 Tz (to ) Tj 0.797222 0 Td 58.333333 Tz (a ) Tj 0.544444 0 Td 68.518519 Tz (Composi ) Tj 51.428571 0 0 51.428571 0 1126.619647 Tm 6.338889 0 Td 47.800926 Tz (tion ) Tj 1.283333 0 Td 58.333333 Tz (2 ) Tj 0.525 0 Td 37.268519 Tz (is ) Tj 0.622222 0 Td 64.814815 Tz (made ) Tj 1.652778 0 Td 56.712963 Tz (of ) Tj 0.952778 0 Td 61.574074 Tz (8 ) Tj 0.525 0 Td 59.953704 Tz (wt ) Tj 0.836111 0 Td 97.222222 Tz (% ) Tj 0.738889 0 Td 48.206019 Tz (alginate ) Tj 2.45 0 Td 46.450617 Tz (\(as ) Tj 0.972222 0 Td 58.873457 Tz (sodium ) Tj 2.255556 0 Td 45.694444 Tz (alginate\); ) Tj 2.955556 0 Td 56.712963 Tz (10 ) Tj 0.816667 0 Td 49.691358 Tz (vol ) Tj 51.428571 0 0 51.428571 0 1085.818955 Tm 6.358333 0 Td 51.851852 Tz (%. ) Tj 0.836111 0 Td 56.481481 Tz (125-150 ) Tj 2.566667 0 Td 71.296296 Tz (um ) Tj 1.147222 0 Td 47.800926 Tz (B-tricalcium ) Tj 3.616667 0 Td 55.452675 Tz (phosphate ) Tj 3.130556 0 Td 48.971193 Tz (granules; ) Tj 2.858333 0 Td 59.953704 Tz (50 ) Tj 0.875 0 Td 68.865741 Tz (vol% ) Tj 1.827778 0 Td 58.333333 Tz (of ) Tj 51.428571 0 0 51.428571 0 1043.363858 Tm 6.358333 0 Td 48.611111 Tz (gel ) Tj 1.069444 0 Td 55.740741 Tz (phase ) Tj 1.866667 0 Td 37.268519 Tz (is ) Tj 0.641667 0 Td 51.851852 Tz (concentrated ) Tj 3.908333 0 Td 44.965278 Tz (platelet ) Tj 2.333333 0 Td 45.046296 Tz (releasate; ) Tj 2.936111 0 Td 55.092593 Tz (5 ) Tj 0.544444 0 Td 98.842593 Tz (mM ) Tj 1.361111 0 Td 52.211934 Tz (trisodium ) Tj 51.428571 0 0 51.428571 0 1003.014194 Tm 6.358333 0 Td 43.981481 Tz (citrate ) Tj 2.119444 0 Td 45.37037 Tz (in ) Tj 0.836111 0 Td 48.611111 Tz (gel ) Tj 1.147222 0 Td 50.771605 Tz (phase; ) Tj 2.158333 0 Td 54.552469 Tz (0.0033 ) Tj 2.275 0 Td 54.62963 Tz (calcium ) Tj 2.625 0 Td 45.833333 Tz (sulfate ) Tj 2.216667 0 Td 45.37037 Tz (\(as ) Tj 1.108333 0 Td 49.583333 Tz (dihydrate\) ) Tj 51.428571 0 0 51.428571 0 962.942857 Tm 6.358333 0 Td 61.033951 Tz (powder ) Tj 2.372222 0 Td 54.552469 Tz (weight ) Tj 2.138889 0 Td 45.37037 Tz (to ) Tj 0.738889 0 Td 48.611111 Tz (gel ) Tj 1.069444 0 Td 56.388889 Tz (phase ) Tj 1.866667 0 Td 59.953704 Tz (Volume ) Tj 2.352778 0 Td 40.509259 Tz (ratio. ) Tj 51.428571 0 0 51.428571 0 922.277019 Tm 7.097222 0 Td 62.654321 Tz (The ) Tj 1.322222 0 Td 51.851852 Tz (implantable ) Tj 3.597222 0 Td 50.231481 Tz (\(ready ) Tj 2.022222 0 Td 48.611111 Tz (for ) Tj 1.05 0 Td 47.890947 Tz (delivery\) ) Tj 2.722222 0 Td 50.378788 Tz (biomaterial ) Tj 3.5 0 Td 69.675926 Tz (compos ) Tj 51.428571 0 0 51.428571 0 876.527181 Tm 6.358333 0 Td 42.12963 Tz (ites ) Tj 1.225 0 Td 50.771605 Tz (are ) Tj 1.186111 0 Td 64.814815 Tz (made ) Tj 1.769444 0 Td 59.953704 Tz (by ) Tj 0.952778 0 Td 52.777778 Tz (passing ) Tj 2.469444 0 Td 48.971193 Tz (materials ) Tj 2.858333 0 Td 59.143519 Tz (back ) Tj 1.633333 0 Td 59.41358 Tz (and ) Tj 1.322222 0 Td 46.018519 Tz (forth ) Tj 1.633333 0 Td 58.333333 Tz (between ) Tj 51.428571 0 0 51.428571 0 837.465786 Tm 6.338889 0 Td 60.493827 Tz (two ) Tj 1.244444 0 Td 54.62963 Tz (coupled ) Tj 2.488889 0 Td 56.17284 Tz (mixing ) Tj 2.177778 0 Td 49.259259 Tz (containers ) Tj 3.130556 0 Td 54.282407 Tz (such ) Tj 1.438889 0 Td 50.231481 Tz (as ) Tj 0.758333 0 Td 47.890947 Tz (Syringes. ) Tj 2.761111 0 Td 66.975309 Tz (One ) Tj 1.361111 0 Td 50.925926 Tz (Syringe ) Tj 51.428571 0 0 51.428571 0 793.140097 Tm 6.358333 0 Td 50.231481 Tz (contains ) Tj 2.547222 0 Td 58.333333 Tz (a ) Tj 0.505556 0 Td 58.873457 Tz (sodium ) Tj 2.294444 0 Td 47.800926 Tz (alginate ) Tj 2.45 0 Td 48.611111 Tz (Solution ) Tj 2.488889 0 Td 45.37037 Tz (at ) Tj 0.661111 0 Td 53.148148 Tz (twice ) Tj 1.730556 0 Td 49.691358 Tz (the ) Tj 1.069444 0 Td 42.12963 Tz (final ) Tj 1.419444 0 Td 63.734568 Tz (concen ) Tj 51.428571 0 0 51.428571 0 751.041816 Tm 6.338889 0 Td 43.75 Tz (tration, ) Tj 2.236111 0 Td 59.41358 Tz (and ) Tj 1.205556 0 Td 50.771605 Tz (the ) Tj 1.011111 0 Td 51.203704 Tz (other ) Tj 1.672222 0 Td 50.462963 Tz (syringe ) Tj 2.255556 0 Td 50.231481 Tz (contains ) Tj 2.547222 0 Td 50.771605 Tz (the ) Tj 1.05 0 Td 54.166667 Tz (calcium ) Tj 2.430556 0 Td 40.509259 Tz (salts. ) Tj 1.594444 0 Td 82.098765 Tz (Con ) Tj 51.428571 0 0 51.428571 0 711.234414 Tm 6.358333 0 Td 47.800926 Tz (centrate ) Tj 2.488889 0 Td 45.37037 Tz (platelet ) Tj 2.391667 0 Td 46.8107 Tz (releasate ) Tj 2.741667 0 Td 37.268519 Tz (is ) Tj 0.661111 0 Td 46.990741 Tz (either ) Tj 1.866667 0 Td 58.333333 Tz (added ) Tj 1.963889 0 Td 45.37037 Tz (directly ) Tj 2.411111 0 Td 45.37037 Tz (to ) Tj 0.758333 0 Td 49.691358 Tz (the ) Tj 1.088889 0 Td 60.493827 Tz (cal ) Tj 51.428571 0 0 51.428571 0 670.513287 Tm 6.358333 0 Td 59.953704 Tz (cium ) Tj 1.963889 0 Td 42.12963 Tz (salt ) Tj 1.516667 0 Td 50.925926 Tz (Syringe ) Tj 2.644444 0 Td 55.092593 Tz (\(Composition ) Tj 4.530556 0 Td 45.37037 Tz (1\) ) Tj 1.05 0 Td 53.472222 Tz (or ) Tj 1.108333 0 Td 59.548611 Tz (combined ) Tj 3.363889 0 Td 54.282407 Tz (with ) Tj 51.428571 0 0 51.428571 0 628.25393 Tm 6.338889 0 Td 51.49177 Tz (trisodium ) Tj 2.994444 0 Td 43.055556 Tz (citrate ) Tj 1.944444 0 Td 46.990741 Tz (in ) Tj 0.719444 0 Td 58.333333 Tz (a ) Tj 0.505556 0 Td 49.826389 Tz (separate ) Tj 2.547222 0 Td 50.925926 Tz (Syringe ) Tj 2.255556 0 Td 60.493827 Tz (and ) Tj 1.244444 0 Td 51.041667 Tz (then ) Tj 1.380556 0 Td 48.021886 Tz (transferred ) Tj 3.325 0 Td 46.990741 Tz (to ) Tj 51.428571 0 0 51.428571 0 588.342857 Tm 6.358333 0 Td 54.166667 Tz (calcium ) Tj 2.527778 0 Td 42.12963 Tz (salt ) Tj 1.225 0 Td 50.925926 Tz (Syringe ) Tj 2.372222 0 Td 55.092593 Tz (\(Composition ) Tj 4.2 0 Td 41.049383 Tz (2\). ) Tj 0.952778 0 Td 64.814815 Tz (The ) Tj 1.361111 0 Td 59.41358 Tz (two ) Tj 1.322222 0 Td 49.826389 Tz (Syringes ) Tj 2.625 0 Td 49.691358 Tz (are ) Tj 51.428571 0 0 51.428571 0 545.249406 Tm 6.338889 0 Td 52.662037 Tz (then ) Tj 1.419444 0 Td 54.62963 Tz (coupled ) Tj 2.411111 0 Td 50.231481 Tz (together ) Tj 2.508333 0 Td 58.333333 Tz (and ) Tj 1.205556 0 Td 48.611111 Tz (materials ) Tj 2.761111 0 Td 49.691358 Tz (are ) Tj 1.030556 0 Td 54.012346 Tz (passed ) Tj 2.041667 0 Td 59.143519 Tz (back ) Tj 1.497222 0 Td 59.41358 Tz (and ) Tj 1.225 0 Td 46.666667 Tz (forth ) Tj 51.428571 0 0 51.428571 0 503.561308 Tm 6.338889 0 Td 57.87037 Tz (between ) Tj 2.838889 0 Td 50.771605 Tz (the ) Tj 1.283333 0 Td 53.472222 Tz (two, ) Tj 1.711111 0 Td 43.75 Tz (initiating ) Tj 2.994444 0 Td 50.771605 Tz (the ) Tj 1.322222 0 Td 46.296296 Tz (setting ) Tj 2.333333 0 Td 46.450617 Tz (reaction. ) Tj 2.955556 0 Td 48.611111 Tz (In ) Tj 0.972222 0 Td 44.560185 Tz (this ) Tj 51.428571 0 0 51.428571 0 461.225032 Tm 6.358333 0 Td 57.449495 Tz (embodiment, ) Tj 4.025 0 Td 49.691358 Tz (the ) Tj 1.108333 0 Td 50.925926 Tz (Syringe ) Tj 2.352778 0 Td 52.932099 Tz (has ) Tj 1.147222 0 Td 58.333333 Tz (a ) Tj 0.583333 0 Td 59.953704 Tz (Volume ) Tj 2.372222 0 Td 56.712963 Tz (of ) Tj 0.797222 0 Td 55.092593 Tz (3 ) Tj 0.583333 0 Td 44.290123 Tz (cc. ) Tj 1.011111 0 Td 63.734568 Tz (The ) Tj 1.361111 0 Td 50.925926 Tz (Syringe ) Tj 51.428571 0 0 51.428571 0 420.075188 Tm 6.358333 0 Td 50.231481 Tz (includes ) Tj 2.586111 0 Td 58.333333 Tz (a ) Tj 0.544444 0 Td 60.925926 Tz (means ) Tj 2.022222 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 49.691358 Tz (attaching ) Tj 2.858333 0 Td 55.092593 Tz (a ) Tj 0.525 0 Td 48.611111 Tz (needle. ) Tj 51.428571 0 0 51.428571 0 379.096076 Tm 7.097222 0 Td 62.654321 Tz (The ) Tj 1.380556 0 Td 50.084175 Tz (biomaterial ) Tj 3.558333 0 Td 55.452675 Tz (composite ) Tj 3.266667 0 Td 37.268519 Tz (is ) Tj 0.738889 0 Td 53.472222 Tz (designed ) Tj 2.8 0 Td 46.990741 Tz (to ) Tj 0.816667 0 Td 59.953704 Tz (be ) Tj 0.991667 0 Td 59.62963 Tz (mixed ) Tj 2.061111 0 Td 43.75 Tz (in ) Tj 0.797222 0 Td 55.092593 Tz (a ) Tj 51.428571 0 0 51.428571 0 338.315855 Tm 6.377778 0 Td 42.12963 Tz (sterile ) Tj 1.963889 0 Td 46.990741 Tz (Surgical ) Tj 2.45 0 Td 46.296296 Tz (setting ) Tj 2.1 0 Td 59.41358 Tz (and ) Tj 1.244444 0 Td 49.691358 Tz (delivered ) Tj 2.858333 0 Td 48.611111 Tz (as ) Tj 0.777778 0 Td 55.092593 Tz (a ) Tj 0.525 0 Td 44.290123 Tz (Sol ) Tj 1.011111 0 Td 53.703704 Tz (through ) Tj 2.430556 0 Td 55.092593 Tz (a ) Tj 0.525 0 Td 64.814815 Tz (mini ) Tj 51.428571 0 0 51.428571 0 295.132005 Tm 6.358333 0 Td 55.092593 Tz (mally ) Tj 1.847222 0 Td 48.611111 Tz (invasive ) Tj 2.527778 0 Td 55.632716 Tz (means, ) Tj 2.216667 0 Td 53.472222 Tz (such ) Tj 1.458333 0 Td 51.851852 Tz (as ) Tj 0.777778 0 Td 58.333333 Tz (an ) Tj 0.894444 0 Td 46.450617 Tz (injection ) Tj 2.702778 0 Td 45.37037 Tz (to ) Tj 0.738889 0 Td 56.712963 Tz (an ) Tj 0.855556 0 Td 53.703704 Tz (osseous ) Tj 2.430556 0 Td 42.939815 Tz (site ) Tj 51.428571 0 0 51.428571 0 252.73456 Tm 6.358333 0 Td 45.37037 Tz (in ) Tj 0.816667 0 Td 51.851852 Tz (a ) Tj 0.544444 0 Td 48.148148 Tz (patient ) Tj 2.255556 0 Td 45.37037 Tz (in ) Tj 0.855556 0 Td 55.902778 Tz (need ) Tj 1.594444 0 Td 45.775463 Tz (thereof, ) Tj 2.469444 0 Td 59.62963 Tz (where ) Tj 2.041667 0 Td 34.027778 Tz (it ) Tj 0.622222 0 Td 49.421296 Tz (gels ) Tj 1.438889 0 Td 49.691358 Tz (for ) Tj 1.108333 0 Td 47.638889 Tz (intentions ) Tj 3.111111 0 Td 58.333333 Tz (of ) Tj 51.428571 0 0 51.428571 0 212.647343 Tm 6.358333 0 Td 48.070988 Tz (repair ) Tj 1.905556 0 Td 52.932099 Tz (and/or ) Tj 2.061111 0 Td 49.96142 Tz (regeneration ) Tj 3.811111 0 Td 58.333333 Tz (of ) Tj 0.797222 0 Td 59.953704 Tz (bone ) Tj 1.613889 0 Td 59.41358 Tz (and ) Tj 1.263889 0 Td 37.808642 Tz (its ) Tj 0.855556 0 Td 50.87963 Tz (associated ) Tj 3.227778 0 Td 45.833333 Tz (tissues ) Tj 51.428571 0 0 51.428571 0 171.960494 Tm 6.377778 0 Td 54.282407 Tz (\(marrow, ) Tj 2.975 0 Td 47.800926 Tz (vasculature, ) Tj 3.752778 0 Td 52.932099 Tz (and/or ) Tj 2.216667 0 Td 50.636574 Tz (adjacent ) Tj 2.761111 0 Td 44.722222 Tz (cartilage, ) Tj 3.033333 0 Td 49.691358 Tz (ligament, ) Tj 30 0 0 30 0 2755 Tm 41.833333 0 Td 72.222222 Tz (5 ) Tj 30 0 0 30 0 2547 Tm 41.666667 0 Td 75 Tz (10 ) Tj 30 0 0 30 0 2339 Tm 41.666667 0 Td 72.222222 Tz (15 ) Tj 30 0 0 30 0 1923 Tm 41.6 0 Td 77.777778 Tz (25 ) Tj 30 0 0 30 0 1716 Tm 41.6 0 Td 80.555556 Tz (30 ) Tj 30 0 0 30 0 1507 Tm 41.6 0 Td 77.777778 Tz (35 ) Tj 30 0 0 30 0 1299 Tm 41.566667 0 Td 83.333333 Tz (40 ) Tj 30 0 0 30 0 1091 Tm 41.566667 0 Td 80.555556 Tz (45 ) Tj 30 0 0 30 0 883 Tm 41.6 0 Td 80.555556 Tz (50 ) Tj 30 0 0 30 0 675 Tm 41.6 0 Td 77.777778 Tz (55 ) Tj 30 0 0 30 0 467 Tm 41.633333 0 Td 77.777778 Tz (60 ) Tj 30 0 0 30 0 259 Tm 41.633333 0 Td 75 Tz (65 ) Tj 52.857143 0 0 52.857143 0 2968 Tm 32.672973 0 Td 74.099099 Tz (10 ) Tj 51.428571 0 0 51.428571 0 2915.998791 Tm 25.316667 0 Td 46.990741 Tz (spinal ) Tj 1.925 0 Td 46.990741 Tz (disc ) Tj 1.341667 0 Td 51.851852 Tz (or ) Tj 0.777778 0 Td 49.421296 Tz (tendon\). ) Tj 2.566667 0 Td 84.259259 Tz (A ) Tj 0.661111 0 Td 48.179012 Tz (therapeutically ) Tj 4.511111 0 Td 47.170782 Tz (effective ) Tj 2.683333 0 Td 61.033951 Tz (amount ) Tj 2.391667 0 Td 56.712963 Tz (of ) Tj 51.428571 0 0 51.428571 0 2873.375447 Tm 25.277778 0 Td 50.771605 Tz (the ) Tj 1.030556 0 Td 53.914141 Tz (composition ) Tj 3.752778 0 Td 37.268519 Tz (is ) Tj 0.583333 0 Td 56.712963 Tz (an ) Tj 0.816667 0 Td 61.033951 Tz (amount ) Tj 2.313889 0 Td 45.37037 Tz (to ) Tj 0.680556 0 Td 54.444444 Tz (cause ) Tj 1.730556 0 Td 50.771605 Tz (the ) Tj 1.030556 0 Td 50 Tz (desired ) Tj 2.216667 0 Td 55.416667 Tz (physiologi ) Tj 51.428571 0 0 51.428571 0 2827.509965 Tm 25.297222 0 Td 46.450617 Tz (cal ) Tj 0.972222 0 Td 42.592593 Tz (effect, ) Tj 1.944444 0 Td 54.282407 Tz (such ) Tj 1.438889 0 Td 50.231481 Tz (as ) Tj 0.7 0 Td 52.175926 Tz (osteogenic ) Tj 3.247222 0 Td 44.965278 Tz (differentiation. ) Tj 4.433333 0 Td 54.282407 Tz (This ) Tj 1.419444 0 Td 50.084175 Tz (biomaterial ) Tj 51.428571 0 0 51.428571 0 2784.297974 Tm 25.297222 0 Td 55.452675 Tz (composite ) Tj 3.111111 0 Td 56.17284 Tz (can ) Tj 1.127778 0 Td 58.333333 Tz (be ) Tj 0.816667 0 Td 55.092593 Tz (used ) Tj 1.438889 0 Td 46.990741 Tz (in ) Tj 0.680556 0 Td 53.472222 Tz (both ) Tj 1.438889 0 Td 53.703704 Tz (medical ) Tj 2.372222 0 Td 60.493827 Tz (and ) Tj 1.225 0 Td 47.260802 Tz (Veterinarian ) Tj 3.558333 0 Td 56.388889 Tz (appli ) Tj 40 0 0 40 0 2747 Tm 32.525 0 Td 59.375 Tz (cations. ) Tj 51.428571 0 0 51.428571 0 2697.965605 Tm 26.036111 0 Td 55.092593 Tz (Underlying ) Tj 3.538889 0 Td 56.018519 Tz (changes ) Tj 2.586111 0 Td 43.75 Tz (in ) Tj 0.797222 0 Td 53.619529 Tz (Subchondral ) Tj 3.772222 0 Td 59.41358 Tz (and ) Tj 1.302778 0 Td 50.87963 Tz (cancellous ) Tj 3.305556 0 Td 59.143519 Tz (bone ) Tj 51.428571 0 0 51.428571 0 2656.399751 Tm 25.297222 0 Td 54.166667 Tz (precede ) Tj 2.45 0 Td 46.666667 Tz (joint ) Tj 1.613889 0 Td 51.851852 Tz (degeneration ) Tj 3.986111 0 Td 46.990741 Tz (in ) Tj 0.797222 0 Td 48.070988 Tz (spinal ) Tj 2.002778 0 Td 44.290123 Tz (discs, ) Tj 1.847222 0 Td 50.771605 Tz (knees, ) Tj 2.080556 0 Td 46.666667 Tz (hips, ) Tj 1.613889 0 Td 60.493827 Tz (and ) Tj 51.428571 0 0 51.428571 0 2614.070039 Tm 25.297222 0 Td 50.555556 Tz (other ) Tj 1.788889 0 Td 43.518519 Tz (joints. ) Tj 2.197222 0 Td 61.574074 Tz (To ) Tj 1.108333 0 Td 58.796296 Tz (example ) Tj 2.819444 0 Td 42.939815 Tz (this ) Tj 1.4 0 Td 55.092593 Tz (a ) Tj 0.680556 0 Td 51.262626 Tz (preliminary ) Tj 3.772222 0 Td 51.311728 Tz (fourteen-day ) Tj 51.428571 0 0 51.428571 0 2565.043915 Tm 25.297222 0 Td 56.17284 Tz (growth ) Tj 2.430556 0 Td 58.333333 Tz (and ) Tj 1.419444 0 Td 52.350427 Tz (osteochondral ) Tj 4.472222 0 Td 45.802469 Tz (differentiation ) Tj 4.511111 0 Td 48.148148 Tz (studies ) Tj 2.430556 0 Td 73.240741 Tz (demon ) Tj 51.428571 0 0 51.428571 0 2525.773585 Tm 25.316667 0 Td 45.833333 Tz (strated ) Tj 2.1 0 Td 49.826389 Tz (biocompatibility ) Tj 4.958333 0 Td 59.41358 Tz (and ) Tj 1.244444 0 Td 46.548822 Tz (bioactivity ) Tj 3.266667 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 56.17284 Tz (Compositions ) Tj 51.428571 0 0 51.428571 0 2480.154791 Tm 25.355556 0 Td 35.648148 Tz (1 ) Tj 0.466667 0 Td 59.41358 Tz (and ) Tj 1.244444 0 Td 58.333333 Tz (2 ) Tj 0.544444 0 Td 45.37037 Tz (in ) Tj 0.758333 0 Td 56.17284 Tz (second ) Tj 2.158333 0 Td 54.62963 Tz (passage ) Tj 2.488889 0 Td 42.12963 Tz (rat ) Tj 0.952778 0 Td 55.092593 Tz (marrow-derived ) Tj 4.841667 0 Td 59.806397 Tz (mesenchymal ) Tj 51.428571 0 0 51.428571 0 2435.290196 Tm 25.316667 0 Td 55.902778 Tz (stem ) Tj 1.691667 0 Td 43.425926 Tz (cells ) Tj 1.633333 0 Td 55.092593 Tz (seeded ) Tj 2.313889 0 Td 52.662037 Tz (onto ) Tj 1.575 0 Td 58.333333 Tz (and ) Tj 1.380556 0 Td 57.253086 Tz (around ) Tj 2.352778 0 Td 53.796296 Tz (cured ) Tj 1.905556 0 Td 49.421296 Tz (gels ) Tj 1.516667 0 Td 51.203704 Tz (containing ) Tj 51.428571 0 0 51.428571 0 2390.626617 Tm 25.277778 0 Td 65.462963 Tz (human ) Tj 2.197222 0 Td 51.851852 Tz (concentrated ) Tj 3.888889 0 Td 44.560185 Tz (platelet ) Tj 2.333333 0 Td 44.722222 Tz (releasate. ) Tj 51.428571 0 0 51.428571 0 2348.353523 Tm 26.016667 0 Td 53.472222 Tz (This ) Tj 1.477778 0 Td 58.796296 Tz (example ) Tj 2.663889 0 Td 51.851852 Tz (introduced ) Tj 3.344444 0 Td 51.851852 Tz (novel ) Tj 1.75 0 Td 44.965278 Tz (platelet ) Tj 2.352778 0 Td 50.517429 Tz (releasate-contain ) Tj 51.428571 0 0 51.428571 0 2307.79669 Tm 25.297222 0 Td 50.771605 Tz (ing ) Tj 1.088889 0 Td 49.826389 Tz (alginate/calcium ) Tj 4.977778 0 Td 54.73251 Tz (phosphate ) Tj 3.111111 0 Td 54.444444 Tz (composites ) Tj 3.461111 0 Td 47.530864 Tz (for ) Tj 0.972222 0 Td 44.560185 Tz (this ) Tj 1.244444 0 Td 54.282407 Tz (interven ) Tj 51.428571 0 0 51.428571 0 2261.817958 Tm 25.277778 0 Td 46.990741 Tz (tion ) Tj 1.341667 0 Td 51.388889 Tz (therapy ) Tj 2.372222 0 Td 58.333333 Tz (and ) Tj 1.225 0 Td 51.49177 Tz (evaluated ) Tj 2.955556 0 Td 59.143519 Tz (them ) Tj 1.672222 0 Td 46.990741 Tz (in ) Tj 0.777778 0 Td 44.074074 Tz (vitro ) Tj 1.536111 0 Td 53.703704 Tz (through ) Tj 2.469444 0 Td 65.219907 Tz (mesenchy ) Tj 51.428571 0 0 51.428571 0 2218.015198 Tm 25.297222 0 Td 60.493827 Tz (mal ) Tj 1.905556 0 Td 55.902778 Tz (stem ) Tj 2.138889 0 Td 43.75 Tz (cell ) Tj 1.827778 0 Td 50.231481 Tz (biocompatibility ) Tj 5.580556 0 Td 60.493827 Tz (and ) Tj 1.886111 0 Td 50.05144 Tz (induction ) Tj 3.519444 0 Td 58.333333 Tz (of ) Tj 51.428571 0 0 51.428571 0 2173.637993 Tm 25.297222 0 Td 52.10114 Tz (osteochondral ) Tj 4.316667 0 Td 44.560185 Tz (differentiation. ) Tj 4.569444 0 Td 82.098765 Tz (DNA ) Tj 1.75 0 Td 47.222222 Tz (quantification ) Tj 4.161111 0 Td 60.493827 Tz (and ) Tj 1.283333 0 Td 58.333333 Tz (gene ) Tj 51.428571 0 0 51.428571 0 2132.992762 Tm 25.297222 0 Td 51.527778 Tz (expression ) Tj 3.286111 0 Td 45.37037 Tz (profiles ) Tj 2.391667 0 Td 53.292181 Tz (suggested ) Tj 3.111111 0 Td 59.511785 Tz (mesenchymal ) Tj 4.180556 0 Td 55.092593 Tz (stem ) Tj 1.575 0 Td 43.425926 Tz (cells ) Tj 1.516667 0 Td 59.143519 Tz (were ) Tj 51.428571 0 0 51.428571 0 2086.184576 Tm 25.297222 0 Td 49.016204 Tz (directed ) Tj 2.741667 0 Td 53.796296 Tz (along ) Tj 2.022222 0 Td 55.092593 Tz (an ) Tj 1.108333 0 Td 51.851852 Tz (osteochondral ) Tj 4.472222 0 Td 45.802469 Tz (differentiation ) Tj 4.511111 0 Td 53.877315 Tz (pathway, ) Tj 51.428571 0 0 51.428571 0 2043.948417 Tm 25.297222 0 Td 62.384259 Tz (more ) Tj 1.652778 0 Td 43.874644 Tz (specifically, ) Tj 3.558333 0 Td 46.990741 Tz (to ) Tj 0.7 0 Td 50.771605 Tz (the ) Tj 1.05 0 Td 58.333333 Tz (immature ) Tj 2.955556 0 Td 52.932099 Tz (nonhypertrophic ) Tj 4.919444 0 Td 62.5 Tz (chondro ) Tj 51.428571 0 0 51.428571 0 1998.255901 Tm 25.297222 0 Td 50.231481 Tz (cyte ) Tj 1.438889 0 Td 53.472222 Tz (phenotype. ) Tj 3.461111 0 Td 58.333333 Tz (These ) Tj 2.022222 0 Td 51.557239 Tz (conclusions ) Tj 3.694444 0 Td 58.333333 Tz (were ) Tj 1.633333 0 Td 55.740741 Tz (based ) Tj 1.944444 0 Td 58.333333 Tz (on ) Tj 0.972222 0 Td 55.092593 Tz (reduced ) Tj 51.428571 0 0 51.428571 0 1959.58156 Tm 25.297222 0 Td 53.652263 Tz (mitogenic ) Tj 3.033333 0 Td 42.849794 Tz (activity, ) Tj 2.45 0 Td 58.333333 Tz (a ) Tj 0.486111 0 Td 50.555556 Tz (rapid ) Tj 1.652778 0 Td 50.501543 Tz (upregulation ) Tj 3.791667 0 Td 53.472222 Tz (followed ) Tj 2.702778 0 Td 59.953704 Tz (by ) Tj 0.894444 0 Td 58.333333 Tz (Suppres ) Tj 51.428571 0 0 51.428571 0 1911.891795 Tm 25.316667 0 Td 50.231481 Tz (sion ) Tj 1.380556 0 Td 55.092593 Tz (of ) Tj 0.758333 0 Td 63.518519 Tz (Runx2 ) Tj 2.080556 0 Td 46.866097 Tz (transcription ) Tj 3.830556 0 Td 48.070988 Tz (factor ) Tj 1.866667 0 Td 74.537037 Tz (mRNA, ) Tj 2.411111 0 Td 50.05144 Tz (Sustained ) Tj 2.858333 0 Td 62.654321 Tz (upregu ) Tj 51.428571 0 0 51.428571 0 1868.716804 Tm 25.297222 0 Td 45.37037 Tz (lation ) Tj 1.788889 0 Td 53.472222 Tz (of ) Tj 0.719444 0 Td 61.574074 Tz (Sox9 ) Tj 1.633333 0 Td 46.828704 Tz (transcription-factor ) Tj 5.755556 0 Td 73.240741 Tz (mRNA, ) Tj 2.352778 0 Td 58.333333 Tz (and ) Tj 1.147222 0 Td 50.771605 Tz (the ) Tj 1.030556 0 Td 55.092593 Tz (absence ) Tj 2.430556 0 Td 58.333333 Tz (of ) Tj 51.428571 0 0 51.428571 0 1823.718519 Tm 25.297222 0 Td 43.75 Tz (late ) Tj 1.341667 0 Td 56.712963 Tz (marker ) Tj 2.255556 0 Td 51.262626 Tz (expressions ) Tj 3.655556 0 Td 48.611111 Tz (for ) Tj 1.069444 0 Td 54.282407 Tz (both ) Tj 1.594444 0 Td 55.632716 Tz (mature ) Tj 2.236111 0 Td 45.730453 Tz (articular ) Tj 2.683333 0 Td 62.5 Tz (chondro ) Tj 51.428571 0 0 51.428571 0 1779.645467 Tm 25.297222 0 Td 49.259259 Tz (cytes ) Tj 1.672222 0 Td 58.333333 Tz (and ) Tj 1.244444 0 Td 48.021886 Tz (osteoblasts ) Tj 3.363889 0 Td 54.282407 Tz (over ) Tj 1.438889 0 Td 49.691358 Tz (the ) Tj 1.069444 0 Td 52.932099 Tz (course ) Tj 2.1 0 Td 58.333333 Tz (of ) Tj 0.797222 0 Td 49.826389 Tz (fourteen ) Tj 2.605556 0 Td 49.259259 Tz (days. ) Tj 51.428571 0 0 51.428571 0 1704.337931 Tm 32.472222 0 Td 61.574074 Tz (Example ) Tj 2.819444 0 Td 38.888889 Tz (1 ) Tj 51.428571 0 0 51.428571 0 1611.138201 Tm 26.036111 0 Td 57.253086 Tz (Rat ) Tj 1.225 0 Td 60.395623 Tz (mesenchymal ) Tj 4.219444 0 Td 55.902778 Tz (stem ) Tj 1.575 0 Td 44.074074 Tz (cells ) Tj 1.555556 0 Td 57.523148 Tz (from ) Tj 1.613889 0 Td 59.41358 Tz (two ) Tj 1.302778 0 Td 54.552469 Tz (donors ) Tj 2.197222 0 Td 58.333333 Tz (which ) Tj 1.983333 0 Td 59.41358 Tz (had ) Tj 51.428571 0 0 51.428571 0 1569.640994 Tm 25.277778 0 Td 57.523148 Tz (been ) Tj 1.652778 0 Td 52.849003 Tz (cryopreserved ) Tj 4.413889 0 Td 59.143519 Tz (upon ) Tj 1.730556 0 Td 51.446759 Tz (reaching ) Tj 2.741667 0 Td 72.376543 Tz (80% ) Tj 1.594444 0 Td 52.824074 Tz (confluence ) Tj 3.422222 0 Td 59.953704 Tz (of ) Tj 0.855556 0 Td 59.41358 Tz (pri ) Tj 51.428571 0 0 51.428571 0 1524.790637 Tm 25.297222 0 Td 61.574074 Tz (mary ) Tj 1.730556 0 Td 47.800926 Tz (cultures ) Tj 2.547222 0 Td 58.981481 Tz (where ) Tj 2.022222 0 Td 59.548611 Tz (combined ) Tj 3.072222 0 Td 45.37037 Tz (at ) Tj 0.758333 0 Td 58.333333 Tz (a ) Tj 0.641667 0 Td 43.209877 Tz (1:1 ) Tj 1.108333 0 Td 43.425926 Tz (ratio ) Tj 1.555556 0 Td 58.333333 Tz (and ) Tj 1.302778 0 Td 54.552469 Tz (seeded ) Tj 2.177778 0 Td 46.990741 Tz (at ) Tj 52.857143 0 0 52.857143 0 1483.3723 Tm 24.689189 0 Td 67.267267 Tz (104 ) Tj 1.437838 0 Td 54.304304 Tz (cells/cm2 ) Tj 3.197297 0 Td 44.144144 Tz (in ) Tj 0.756757 0 Td 43.793794 Tz (T-flasks. ) Tj 2.610811 0 Td 47.927928 Tz (Cells ) Tj 1.702703 0 Td 56.756757 Tz (were ) Tj 1.589189 0 Td 53.209459 Tz (passaged ) Tj 2.781081 0 Td 42.567568 Tz (at ) Tj 0.718919 0 Td 72.522523 Tz (80% ) Tj 1.532432 0 Td 56.756757 Tz (of ) Tj 51.428571 0 0 51.428571 0 1445.168766 Tm 25.277778 0 Td 43.75 Tz (this ) Tj 1.341667 0 Td 37.592593 Tz (first ) Tj 1.361111 0 Td 54.166667 Tz (passage ) Tj 2.508333 0 Td 59.41358 Tz (and ) Tj 1.322222 0 Td 55.902778 Tz (used ) Tj 1.594444 0 Td 48.611111 Tz (for ) Tj 1.108333 0 Td 52.067901 Tz (experimentation ) Tj 4.977778 0 Td 58.333333 Tz (upon ) Tj 1.672222 0 Td 53.796296 Tz (again ) Tj 51.428571 0 0 51.428571 0 1403.456576 Tm 25.297222 0 Td 52.256944 Tz (reaching ) Tj 2.741667 0 Td 62.384259 Tz (80%. ) Tj 1.75 0 Td 59.953704 Tz (At ) Tj 0.952778 0 Td 49.691358 Tz (the ) Tj 1.127778 0 Td 53.652263 Tz (beginning ) Tj 3.15 0 Td 58.333333 Tz (of ) Tj 0.816667 0 Td 55.902778 Tz (each ) Tj 1.575 0 Td 55.416667 Tz (experiment ) Tj 3.558333 0 Td 74.537037 Tz (MSCs ) Tj 51.428571 0 0 51.428571 0 1359.054591 Tm 25.297222 0 Td 58.333333 Tz (from ) Tj 1.613889 0 Td 59.41358 Tz (two ) Tj 1.302778 0 Td 54.552469 Tz (donors ) Tj 2.177778 0 Td 58.333333 Tz (were ) Tj 1.594444 0 Td 59.548611 Tz (combined ) Tj 3.052778 0 Td 58.333333 Tz (and ) Tj 1.225 0 Td 58.333333 Tz (expanded ) Tj 3.013889 0 Td 48.611111 Tz (for ) Tj 1.05 0 Td 57.253086 Tz (one ) Tj 1.205556 0 Td 68.055556 Tz (pas ) Tj 51.428571 0 0 51.428571 0 1320.724566 Tm 25.316667 0 Td 53.472222 Tz (sage ) Tj 1.497222 0 Td 46.990741 Tz (in ) Tj 0.816667 0 Td 57.973251 Tz (monolayer ) Tj 3.325 0 Td 58.333333 Tz (and ) Tj 1.302778 0 Td 58.333333 Tz (were ) Tj 1.613889 0 Td 50.231481 Tz (entering ) Tj 2.605556 0 Td 46.018519 Tz (their ) Tj 1.594444 0 Td 56.17284 Tz (second ) Tj 2.236111 0 Td 50.636574 Tz (passage. ) Tj 51.428571 0 0 51.428571 0 1277.703524 Tm 25.297222 0 Td 48.611111 Tz (Cells ) Tj 1.691667 0 Td 58.333333 Tz (were ) Tj 1.555556 0 Td 62.87037 Tz (grown ) Tj 2.061111 0 Td 48.611111 Tz (in ) Tj 0.816667 0 Td 70.216049 Tz (10% ) Tj 1.477778 0 Td 47.314815 Tz (\(v/v\) ) Tj 1.633333 0 Td 57.523148 Tz (FBS-supplemented ) Tj 5.755556 0 Td 102.893519 Tz (DMEM ) Tj 51.428571 0 0 51.428571 0 1239.95098 Tm 25.316667 0 Td 84.259259 Tz (HG ) Tj 1.361111 0 Td 50.87963 Tz (containing ) Tj 3.363889 0 Td 44.191919 Tz (penicillin, ) Tj 3.247222 0 Td 50.605413 Tz (Streptomycin, ) Tj 4.258333 0 Td 59.41358 Tz (and ) Tj 1.361111 0 Td 53.742284 Tz (amphotericin ) Tj 51.428571 0 0 51.428571 0 1191 Tm 25.277778 0 Td 56.388889 Tz (under ) Tj 1.808333 0 Td 56.17284 Tz (normal ) Tj 2.119444 0 Td 49.074074 Tz (culture ) Tj 2.158333 0 Td 50.231481 Tz (conditions ) Tj 3.169444 0 Td 49.421296 Tz (\(37 ) Tj 1.322222 0 Td 48.611111 Tz (C.; ) Tj 1.011111 0 Td 58.796296 Tz (5%\(v/v\) ) Tj 2.586111 0 Td 83.179012 Tz (CO; ) Tj 1.672222 0 Td 68.055556 Tz (100% ) Tj 51.428571 0 0 51.428571 0 1149 Tm 25.297222 0 Td 58.333333 Tz (RH\). ) Tj 51.428571 0 0 51.428571 0 1113.929293 Tm 26.036111 0 Td 54.282407 Tz (Experimental ) Tj 4.044444 0 Td 57.154882 Tz (Composition ) Tj 3.986111 0 Td 38.888889 Tz (1 ) Tj 0.447222 0 Td 59.41358 Tz (and ) Tj 1.225 0 Td 56.565657 Tz (Composition ) Tj 3.927778 0 Td 55.092593 Tz (2 ) Tj 0.505556 0 Td 50.771605 Tz (\(Table ) Tj 2.061111 0 Td 43.75 Tz (1\) ) Tj 51.428571 0 0 51.428571 0 1068.666667 Tm 25.277778 0 Td 59.953704 Tz (were ) Tj 1.652778 0 Td 58.333333 Tz (formed ) Tj 2.294444 0 Td 46.990741 Tz (in ) Tj 0.758333 0 Td 50.771605 Tz (3.6 ) Tj 1.186111 0 Td 59.953704 Tz (ml ) Tj 0.933333 0 Td 58.333333 Tz (and ) Tj 1.263889 0 Td 47.530864 Tz (3.3 ) Tj 1.147222 0 Td 59.953704 Tz (ml ) Tj 0.952778 0 Td 42.592593 Tz (sterile ) Tj 1.963889 0 Td 45.664983 Tz (quantities, ) Tj 3.266667 0 Td 57.253086 Tz (respec ) Tj 51.428571 0 0 51.428571 0 1027.214748 Tm 25.277778 0 Td 41.666667 Tz (tively. ) Tj 2.002778 0 Td 58.333333 Tz (Calcium ) Tj 2.702778 0 Td 42.12963 Tz (salts ) Tj 1.536111 0 Td 58.333333 Tz (were ) Tj 1.613889 0 Td 54.012346 Tz (loaded ) Tj 2.177778 0 Td 45.37037 Tz (in ) Tj 0.797222 0 Td 43.75 Tz (to ) Tj 0.758333 0 Td 55.092593 Tz (3 ) Tj 0.583333 0 Td 53.472222 Tz (cc ) Tj 0.894444 0 Td 50.231481 Tz (syringes ) Tj 2.663889 0 Td 58.981481 Tz (which ) Tj 51.428571 0 0 51.428571 0 984.037736 Tm 25.277778 0 Td 59.953704 Tz (were ) Tj 1.555556 0 Td 44.722222 Tz (sterilized ) Tj 2.8 0 Td 52.662037 Tz (with ) Tj 1.419444 0 Td 71.296296 Tz (gamma ) Tj 2.275 0 Td 45.664983 Tz (irradiation ) Tj 3.169444 0 Td 50.555556 Tz (\(-2.5 ) Tj 1.691667 0 Td 54.012346 Tz (Mrad\). ) Tj 2.080556 0 Td 69.135802 Tz (Concen ) Tj 51.428571 0 0 51.428571 0 943.085575 Tm 25.277778 0 Td 45.910494 Tz (trated ) Tj 1.847222 0 Td 66.111111 Tz (human ) Tj 2.197222 0 Td 44.965278 Tz (platelet ) Tj 2.372222 0 Td 46.8107 Tz (releasate ) Tj 2.702778 0 Td 62.114198 Tz (\(hCPR\) ) Tj 2.411111 0 Td 58.333333 Tz (and ) Tj 1.263889 0 Td 54.444444 Tz (water ) Tj 1.827778 0 Td 58.333333 Tz (were ) Tj 1.594444 0 Td 52.662037 Tz (ster ) Tj 51.428571 0 0 51.428571 0 902.446941 Tm 25.297222 0 Td 44.830247 Tz (ilized ) Tj 1.827778 0 Td 54.166667 Tz (through ) Tj 2.508333 0 Td 50.771605 Tz (0.2 ) Tj 1.205556 0 Td 71.296296 Tz (um ) Tj 1.127778 0 Td 40.06734 Tz (filtration. ) Tj 2.877778 0 Td 51.851852 Tz (Alginate ) Tj 2.722222 0 Td 48.611111 Tz (solutions ) Tj 2.838889 0 Td 46.990741 Tz (at ) Tj 0.777778 0 Td 53.148148 Tz (twice ) Tj 51.428571 0 0 51.428571 0 861.382199 Tm 25.277778 0 Td 50.771605 Tz (the ) Tj 1.225 0 Td 43.425926 Tz (final ) Tj 1.652778 0 Td 54.012346 Tz (weight ) Tj 2.275 0 Td 52.5 Tz (percentage ) Tj 3.5 0 Td 49.421296 Tz (\(per ) Tj 1.458333 0 Td 53.796296 Tz (Table ) Tj 2.002778 0 Td 45.37037 Tz (1\) ) Tj 0.894444 0 Td 58.333333 Tz (were ) Tj 1.691667 0 Td 51.851852 Tz (autoclave ) Tj 51.428571 0 0 51.428571 0 818.944107 Tm 25.316667 0 Td 44.074074 Tz (sterilized ) Tj 2.761111 0 Td 48.611111 Tz (for ) Tj 1.011111 0 Td 55.092593 Tz (15 ) Tj 0.797222 0 Td 54.166667 Tz (minutes ) Tj 2.372222 0 Td 46.990741 Tz (at ) Tj 0.738889 0 Td 52.662037 Tz (120 ) Tj 1.419444 0 Td 55.092593 Tz (C. ) Tj 0.777778 0 Td 58.333333 Tz (on ) Tj 0.836111 0 Td 49.691358 Tz (the ) Tj 0.991667 0 Td 46.759259 Tz (liquids ) Tj 2.119444 0 Td 45.833333 Tz (setting ) Tj 2.041667 0 Td 52.5 Tz (\(slow ) Tj 51.428571 0 0 51.428571 0 776.131611 Tm 25.297222 0 Td 45.37037 Tz (vent\). ) Tj 1.808333 0 Td 55.452675 Tz (Trisodium ) Tj 3.208333 0 Td 43.518519 Tz (citrate ) Tj 2.022222 0 Td 60.493827 Tz (was ) Tj 1.302778 0 Td 44.398148 Tz (sterilized ) Tj 2.858333 0 Td 53.240741 Tz (through ) Tj 2.45 0 Td 49.691358 Tz (0.2 ) Tj 1.108333 0 Td 48.611111 Tz (Lum ) Tj 1.108333 0 Td 44.290123 Tz (filtra ) Tj 51.428571 0 0 51.428571 0 736.406659 Tm 25.277778 0 Td 46.990741 Tz (tion ) Tj 1.244444 0 Td 51.851852 Tz (as ) Tj 0.738889 0 Td 51.851852 Tz (a ) Tj 0.466667 0 Td 48.611111 Tz (solution ) Tj 2.430556 0 Td 53.877315 Tz (followed ) Tj 2.683333 0 Td 59.953704 Tz (by ) Tj 0.875 0 Td 41.666667 Tz (sterile ) Tj 1.886111 0 Td 46.666667 Tz (lyophillization ) Tj 4.336111 0 Td 48.611111 Tz (for ) Tj 0.991667 0 Td 60.925926 Tz (recon ) Tj 51.428571 0 0 51.428571 0 694.461538 Tm 25.316667 0 Td 43.569959 Tz (stitution ) Tj 2.527778 0 Td 46.990741 Tz (at ) Tj 0.719444 0 Td 54.282407 Tz (time ) Tj 1.497222 0 Td 56.712963 Tz (of ) Tj 0.816667 0 Td 51.851852 Tz (mixing. ) Tj 38.571429 0 0 38.571429 0 613 Tm 43.477778 0 Td 101.975309 Tz (TABLE ) Tj 3.396296 0 Td 47.530864 Tz (1 ) Tj 40 0 0 40 0 546 Tm 41.725 0 Td 55.902778 Tz (Compositions ) Tj 31.428571 0 0 31.428571 0 480 Tm 66.690909 0 Td 75 Tz (Calcium ) Tj 30.952381 0 0 30.952381 0 446 Tm 60.415385 0 Td 72.692308 Tz (B- ) Tj 3.230769 0 Td 78.974359 Tz (Sodium ) Tj 4.361538 0 Td 62.307692 Tz (Sulfate ) Tj 40 0 0 40 0 411.00396 Tm 32.525 0 Td 56.439394 Tz (Composition ) Tj 4.425 0 Td 52.777778 Tz (Liquid ) Tj 9.275 0 Td 73.611111 Tz (TCP ) Tj 2.525 0 Td 51.851852 Tz (Alginate: ) Tj 2.9 0 Td 45.833333 Tz (* ) Tj 0.675 0 Td 61.904762 Tz (\(LP***\) ) Tj 32.727273 0 0 32.727273 0 349.265823 Tm 39.813889 0 Td 50.925926 Tz (1 ) Tj 0.641667 0 Td 61.111111 Tz (or ) Tj 0.916667 0 Td 78.08642 Tz (F1+ ) Tj 4.155556 0 Td 89.969136 Tz (50% ) Tj 1.894444 0 Td 56.018519 Tz (\(v/v\) ) Tj 1.894444 0 Td 61.111111 Tz (water: ) Tj 7.455556 0 Td 71.296296 Tz (2O ) Tj 3.513889 0 Td 66.203704 Tz (2 ) Tj 0.641667 0 Td 78.935185 Tz (wt ) Tj 1.191667 0 Td 101.851852 Tz (% ) Tj 2.75 0 Td 72.993827 Tz (300 ) Tj 37.857143 0 0 37.857143 0 314.645161 Tm 39.358491 0 Td 77.777778 Tz (50% ) Tj 1.637736 0 Td 49.308176 Tz (\(v/v\) ) Tj 1.664151 0 Td 78.144654 Tz (hCPR ) Tj 6.154717 0 Td 74.842767 Tz (vol% ) Tj 32.727273 0 0 32.727273 0 282.265823 Tm 39.752778 0 Td 71.296296 Tz (2 ) Tj 0.702778 0 Td 61.111111 Tz (or ) Tj 0.947222 0 Td 78.08642 Tz (F2+ ) Tj 4.125 0 Td 89.969136 Tz (50% ) Tj 1.894444 0 Td 56.018519 Tz (\(v/v\) ) Tj 1.894444 0 Td 61.111111 Tz (water: ) Tj 7.516667 0 Td 66.203704 Tz (10 ) Tj 3.483333 0 Td 66.203704 Tz (8 ) Tj 0.641667 0 Td 76.388889 Tz (wt ) Tj 1.130556 0 Td 106.944444 Tz (% ) Tj 2.841667 0 Td 69.598765 Tz (100 ) Tj 37.857143 0 0 37.857143 0 247.612648 Tm 39.358491 0 Td 77.777778 Tz (50% ) Tj 1.637736 0 Td 49.308176 Tz (\(v/v\) ) Tj 1.664151 0 Td 66.918239 Tz (hCPR: ) Tj 6.154717 0 Td 74.842767 Tz (vol% ) Tj 31.428571 0 0 31.428571 0 219 Tm 46.963636 0 Td 68.939394 Tz (5 ) Tj 0.668182 0 Td 70.055821 Tz (mMtrisodium-citrate ) Tj ET Q endstream endobj 460 0 obj << /Length 1 >> stream q endstream endobj 461 0 obj << /Length 48750 >> stream Q q 0.24 0 0 0.24 0 0 cm BT /F1 1 Tf 3 Tr 62.857143 0 0 62.857143 0 3023 Tm 17.325 0 Td 83.522727 Tz (US ) Tj 1.240909 0 Td 58.333333 Tz (8,404,256 ) Tj 3.388636 0 Td 74.242424 Tz (B2 ) Tj 51.428571 0 0 51.428571 0 2969 Tm 14.661111 0 Td 71.296296 Tz (11 ) Tj 40 0 0 40 0 2913 Tm 15.65 0 Td 97.5 Tz (TABLE ) Tj 3.225 0 Td 67.045455 Tz (1-continued ) Tj 40 0 0 40 0 2846 Tm 17.425 0 Td 56.25 Tz (Compositions ) Tj 31.428571 0 0 31.428571 0 2780 Tm 35.7 0 Td 75 Tz (Calcium ) Tj 30.952381 0 0 30.952381 0 2747 Tm 28.98 0 Td 70 Tz (B- ) Tj 3.198462 0 Td 78.974359 Tz (Sodium ) Tj 4.361538 0 Td 62.307692 Tz (Sulfate ) Tj 40 0 0 40 0 2711.011905 Tm 8.175 0 Td 56.439394 Tz (Composition ) Tj 4.425 0 Td 52.777778 Tz (Liquid ) Tj 9.275 0 Td 73.611111 Tz (TCP ) Tj 2.525 0 Td 51.851852 Tz (Alginate: ) Tj 2.9 0 Td 45.833333 Tz (* ) Tj 0.7 0 Td 61.309524 Tz (\(LP***\) ) Tj 34.444444 0 0 34.444444 0 2647.946281 Tm 9.493548 0 Td 61.143695 Tz (F1-Material ) Tj 5.109677 0 Td 79.83871 Tz (100% ) Tj 2.148387 0 Td 54.193548 Tz (\(v/v\) ) Tj 1.829032 0 Td 63.870968 Tz (water ) Tj 7.025806 0 Td 80.645161 Tz (20% ) Tj 3.396774 0 Td 62.903226 Tz (2 ) Tj 0.609677 0 Td 75 Tz (wt ) Tj 1.132258 0 Td 96.774194 Tz (% ) Tj 2.612903 0 Td 69.354839 Tz (300 ) Tj 31.428571 0 0 31.428571 0 2619 Tm 10.404545 0 Td 67.424242 Tz (Control ) Tj 36.666667 0 0 36.666667 0 2545.596264 Tm 8.945455 0 Td 57.792208 Tz (*Volume ) Tj 2.563636 0 Td 48.701299 Tz (percent ) Tj 2.209091 0 Td 51.818182 Tz (based ) Tj 1.718182 0 Td 49.090909 Tz (on-B-tricalcium ) Tj 4.581818 0 Td 51.515152 Tz (phosphate ) Tj 2.945455 0 Td 48.051948 Tz (density ) Tj 2.154545 0 Td 54.545455 Tz (of ) Tj 0.709091 0 Td 50 Tz (3.14 ) Tj 1.363636 0 Td 62.727273 Tz (g/cm3 ) Tj 34.285714 0 0 34.285714 0 2519.459016 Tm 9.566667 0 Td 60.763889 Tz (**wt ) Tj 1.575 0 Td 106.944444 Tz (% ) Tj 0.816667 0 Td 56.388889 Tz (based ) Tj 1.8375 0 Td 60.763889 Tz (on ) Tj 0.875 0 Td 51.851852 Tz (gel ) Tj 1.108333 0 Td 50 Tz (portion ) Tj 2.275 0 Td 55.902778 Tz (only ) Tj 34.285714 0 0 34.285714 0 2485.131579 Tm 9.566667 0 Td 70.972222 Tz (***LP ) Tj 2.304167 0 Td 41.319444 Tz (is ) Tj 0.7 0 Td 51.527778 Tz (equal ) Tj 1.720833 0 Td 46.180556 Tz (to ) Tj 0.758333 0 Td 48.611111 Tz (gel ) Tj 1.020833 0 Td 61.574074 Tz (volume ) Tj 2.391667 0 Td 46.180556 Tz (in ) Tj 0.758333 0 Td 42.866162 Tz (milliliters ) Tj 3.033333 0 Td 51.388889 Tz (divided ) Tj 2.304167 0 Td 65.625 Tz (by ) Tj 0.933333 0 Td 54.166667 Tz (calcium ) Tj 2.479167 0 Td 45.833333 Tz (sulfate ) Tj 2.1 0 Td 60.763889 Tz (mass ) Tj 1.633333 0 Td 46.180556 Tz (in ) Tj 0.729167 0 Td 60.277778 Tz (grams ) Tj 34.285714 0 0 34.285714 0 2453.760814 Tm 9.566667 0 Td 55.902778 Tz (****Molarity ) Tj 4.170833 0 Td 38.888889 Tz (is ) Tj 0.6125 0 Td 57.361111 Tz (based ) Tj 1.866667 0 Td 60.763889 Tz (on ) Tj 0.904167 0 Td 50.231481 Tz (gel ) Tj 1.05 0 Td 61.574074 Tz (volume ) Tj 2.391667 0 Td 46.666667 Tz (only, ) Tj 51.428571 0 0 51.428571 0 2376.318681 Tm 7.116667 0 Td 46.990741 Tz (In ) Tj 0.836111 0 Td 51.851852 Tz (a ) Tj 0.583333 0 Td 42.12963 Tz (cell ) Tj 1.302778 0 Td 48.148148 Tz (culture ) Tj 2.255556 0 Td 61.574074 Tz (hood ) Tj 1.788889 0 Td 46.450617 Tz (1.5 ) Tj 1.147222 0 Td 59.953704 Tz (ml ) Tj 0.991667 0 Td 59.953704 Tz (of ) Tj 0.855556 0 Td 42.592593 Tz (sterile ) Tj 2.022222 0 Td 48.611111 Tz (alginate ) Tj 2.586111 0 Td 49.421296 Tz (solution ) Tj 51.428571 0 0 51.428571 0 2332.249666 Tm 6.338889 0 Td 59.953704 Tz (were ) Tj 1.711111 0 Td 54.012346 Tz (loaded ) Tj 2.216667 0 Td 46.990741 Tz (into ) Tj 1.4 0 Td 55.092593 Tz (a ) Tj 0.622222 0 Td 42.12963 Tz (sterile ) Tj 2.041667 0 Td 51.851852 Tz (3 ) Tj 0.641667 0 Td 51.851852 Tz (cc ) Tj 0.952778 0 Td 47.395833 Tz (syringe. ) Tj 2.586111 0 Td 45.37037 Tz (Sterile ) Tj 2.158333 0 Td 72.106481 Tz (hCPR ) Tj 2.041667 0 Td 61.574074 Tz (was ) Tj 51.428571 0 0 51.428571 0 2292.686642 Tm 6.358333 0 Td 58.333333 Tz (added ) Tj 1.925 0 Td 45.37037 Tz (to ) Tj 0.758333 0 Td 51.851852 Tz (a ) Tj 0.525 0 Td 42.12963 Tz (sterile ) Tj 1.925 0 Td 58.333333 Tz (3 ) Tj 0.563889 0 Td 53.472222 Tz (cc ) Tj 0.836111 0 Td 51.388889 Tz (syringe ) Tj 2.333333 0 Td 51.203704 Tz (containing ) Tj 3.266667 0 Td 49.691358 Tz (the ) Tj 1.088889 0 Td 42.12963 Tz (sterile ) Tj 1.944444 0 Td 55.092593 Tz (calcium ) Tj 51.428571 0 0 51.428571 0 2249.92279 Tm 6.377778 0 Td 40.509259 Tz (salts; ) Tj 1.652778 0 Td 45.37037 Tz (in ) Tj 0.738889 0 Td 48.611111 Tz (the ) Tj 1.030556 0 Td 51.851852 Tz (case ) Tj 1.419444 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 56.860269 Tz (Composition ) Tj 3.947222 0 Td 58.333333 Tz (2 ) Tj 0.525 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 72.106481 Tz (hCPR ) Tj 1.925 0 Td 60.493827 Tz (was ) Tj 1.263889 0 Td 38.240741 Tz (first ) Tj 1.322222 0 Td 60.925926 Tz (mixed ) Tj 51.428571 0 0 51.428571 0 2209.211334 Tm 6.338889 0 Td 54.282407 Tz (with ) Tj 1.497222 0 Td 48.611111 Tz (the ) Tj 1.011111 0 Td 50.673401 Tz (appropriate ) Tj 3.480556 0 Td 61.574074 Tz (amount ) Tj 2.352778 0 Td 59.953704 Tz (of ) Tj 0.797222 0 Td 40.509259 Tz (sterile, ) Tj 2.138889 0 Td 49.494949 Tz (lyophilized ) Tj 3.422222 0 Td 51.851852 Tz (trisodium ) Tj 51.428571 0 0 51.428571 0 2167.039801 Tm 6.358333 0 Td 43.518519 Tz (citrate ) Tj 2.041667 0 Td 52.391975 Tz (before ) Tj 2.080556 0 Td 55.092593 Tz (being ) Tj 1.886111 0 Td 60.277778 Tz (mixed ) Tj 2.022222 0 Td 53.472222 Tz (with ) Tj 1.555556 0 Td 53.703704 Tz (calcium ) Tj 2.527778 0 Td 39.969136 Tz (salts. ) Tj 1.672222 0 Td 63.734568 Tz (The ) Tj 1.361111 0 Td 48.611111 Tz (alginate ) Tj 51.428571 0 0 51.428571 0 2126.690107 Tm 6.358333 0 Td 51.203704 Tz (containing ) Tj 3.247222 0 Td 51.388889 Tz (Syringe ) Tj 2.313889 0 Td 60.493827 Tz (was ) Tj 1.244444 0 Td 52.662037 Tz (then ) Tj 1.458333 0 Td 54.62963 Tz (coupled ) Tj 2.45 0 Td 53.472222 Tz (with ) Tj 1.458333 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 59.143519 Tz (hCPR/calcium ) Tj 51.428571 0 0 51.428571 0 2085.46472 Tm 6.377778 0 Td 42.12963 Tz (salt ) Tj 1.166667 0 Td 50.925926 Tz (Syringe ) Tj 2.294444 0 Td 58.333333 Tz (and ) Tj 1.205556 0 Td 51.851852 Tz (curing ) Tj 2.022222 0 Td 61.574074 Tz (was ) Tj 1.283333 0 Td 43.930041 Tz (initiated ) Tj 2.508333 0 Td 61.574074 Tz (by ) Tj 0.913889 0 Td 51.851852 Tz (passing ) Tj 2.333333 0 Td 49.691358 Tz (the ) Tj 1.088889 0 Td 48.611111 Tz (materials ) Tj 51.428571 0 0 51.428571 0 2042.232558 Tm 6.338889 0 Td 59.143519 Tz (back ) Tj 1.555556 0 Td 58.333333 Tz (and ) Tj 1.244444 0 Td 47.314815 Tz (forth ) Tj 1.575 0 Td 57.87037 Tz (between ) Tj 2.625 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 50.231481 Tz (syringes ) Tj 2.663889 0 Td 55.740741 Tz (15-20 ) Tj 1.808333 0 Td 48.148148 Tz (passes. ) Tj 2.177778 0 Td 51.851852 Tz (All ) Tj 1.088889 0 Td 59.953704 Tz (of ) Tj 0.797222 0 Td 50.771605 Tz (the ) Tj 51.428571 0 0 51.428571 0 2002.91198 Tm 6.358333 0 Td 51.388889 Tz (content ) Tj 2.352778 0 Td 61.574074 Tz (was ) Tj 1.302778 0 Td 50.555556 Tz (positioned ) Tj 3.266667 0 Td 43.75 Tz (in ) Tj 0.758333 0 Td 58.333333 Tz (one ) Tj 1.225 0 Td 48.611111 Tz (syringe, ) Tj 2.488889 0 Td 50.231481 Tz (junction ) Tj 2.625 0 Td 52.314815 Tz (broken, ) Tj 2.391667 0 Td 60.493827 Tz (and ) Tj 51.428571 0 0 51.428571 0 1960.98005 Tm 6.416667 0 Td 55.497685 Tz (16-gauge ) Tj 2.838889 0 Td 52.932099 Tz (needle ) Tj 2.041667 0 Td 53.472222 Tz (added. ) Tj 2.177778 0 Td 35.648148 Tz (1 ) Tj 0.505556 0 Td 63.194444 Tz (ml ) Tj 0.933333 0 Td 47.800926 Tz (aliquots ) Tj 2.488889 0 Td 59.953704 Tz (of ) Tj 0.777778 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 49.691358 Tz (gel ) Tj 1.108333 0 Td 58.333333 Tz (were ) Tj 1.594444 0 Td 52.932099 Tz (spread ) Tj 51.428571 0 0 51.428571 0 1917.436548 Tm 6.358333 0 Td 53.796296 Tz (completely ) Tj 3.5 0 Td 54.282407 Tz (over ) Tj 1.555556 0 Td 48.611111 Tz (the ) Tj 1.186111 0 Td 49.074074 Tz (surface ) Tj 2.333333 0 Td 59.953704 Tz (of ) Tj 0.952778 0 Td 50.925926 Tz (12-well ) Tj 2.430556 0 Td 48.148148 Tz (culture ) Tj 2.275 0 Td 47.962963 Tz (plate ) Tj 1.691667 0 Td 51.203704 Tz (wells ) Tj 51.428571 0 0 51.428571 0 1877.29816 Tm 6.338889 0 Td 54.166667 Tz (through ) Tj 2.45 0 Td 44.398148 Tz (injection. ) Tj 2.877778 0 Td 55.092593 Tz (Gels ) Tj 1.477778 0 Td 59.143519 Tz (were ) Tj 1.555556 0 Td 53.148148 Tz (given ) Tj 1.75 0 Td 55.092593 Tz (a ) Tj 0.525 0 Td 65.740741 Tz (minimum ) Tj 2.994444 0 Td 55.092593 Tz (15 ) Tj 0.875 0 Td 53.703704 Tz (minutes ) Tj 2.411111 0 Td 46.990741 Tz (to ) Tj 40 0 0 40 0 1840 Tm 8.175 0 Td 67.708333 Tz (cure ) Tj 1.85 0 Td 56.25 Tz (in ) Tj 0.95 0 Td 47.916667 Tz (air. ) Tj 51.428571 0 0 51.428571 0 1795.812261 Tm 7.097222 0 Td 52.662037 Tz (This ) Tj 1.477778 0 Td 48.206019 Tz (resulted ) Tj 2.527778 0 Td 43.75 Tz (in ) Tj 0.738889 0 Td 56.712963 Tz (an ) Tj 0.894444 0 Td 50 Tz (alginate-based ) Tj 4.413889 0 Td 55.092593 Tz (composite ) Tj 3.188889 0 Td 55.092593 Tz (system ) Tj 2.197222 0 Td 71.296296 Tz (con ) Tj 51.428571 0 0 51.428571 0 1752.881936 Tm 6.338889 0 Td 48.611111 Tz (taining ) Tj 2.236111 0 Td 56.018519 Tz (125-150 ) Tj 2.527778 0 Td 71.296296 Tz (um ) Tj 1.127778 0 Td 47.530864 Tz (B-tricalcium ) Tj 3.577778 0 Td 55.092593 Tz (phosphate ) Tj 3.072222 0 Td 61.574074 Tz (\(TCP\) ) Tj 1.944444 0 Td 49.583333 Tz (aggregate. ) Tj 51.428571 0 0 51.428571 0 1710.933985 Tm 6.358333 0 Td 54.012346 Tz (Osteoconductive ) Tj 5.133333 0 Td 51.446759 Tz (granules ) Tj 2.741667 0 Td 55.555556 Tz (\(Plasma ) Tj 2.663889 0 Td 47.530864 Tz (Biotal ) Tj 2.041667 0 Td 48.611111 Tz (Ltd; ) Tj 1.497222 0 Td 50.968013 Tz (Derbyshire, ) Tj 51.428571 0 0 51.428571 0 1668.989117 Tm 6.377778 0 Td 70.216049 Tz (UK\) ) Tj 1.458333 0 Td 59.143519 Tz (were ) Tj 1.594444 0 Td 51.311728 Tz (incorporated ) Tj 3.869444 0 Td 50.771605 Tz (within ) Tj 2.041667 0 Td 51.851852 Tz (a ) Tj 0.525 0 Td 52.824074 Tz (continuous ) Tj 3.363889 0 Td 48.206019 Tz (alginate ) Tj 2.488889 0 Td 49.537037 Tz (\(Fluka; ) Tj 52.857143 0 0 52.857143 0 1624.365854 Tm 6.205405 0 Td 39.93994 Tz (St. ) Tj 1.021622 0 Td 48.873874 Tz (Louis, ) Tj 2.024324 0 Td 59.121622 Tz (Mo.\) ) Tj 1.645946 0 Td 52.421171 Tz (hydrogel ) Tj 2.8 0 Td 47.747748 Tz (matrix. ) Tj 2.251351 0 Td 81.981982 Tz (A ) Tj 0.756757 0 Td 50.45045 Tz (small ) Tj 1.759459 0 Td 59.384384 Tz (amount ) Tj 2.402703 0 Td 55.18018 Tz (of ) Tj 0.813514 0 Td 50.45045 Tz (Terra ) Tj 51.428571 0 0 51.428571 0 1583.355247 Tm 6.358333 0 Td 58.333333 Tz (Alba ) Tj 1.652778 0 Td 54.62963 Tz (calcium ) Tj 2.586111 0 Td 45.833333 Tz (sulfate ) Tj 2.177778 0 Td 62.222222 Tz (\(USG; ) Tj 2.158333 0 Td 54.282407 Tz (Chicago, ) Tj 2.877778 0 Td 35.648148 Tz (Ill.\) ) Tj 1.302778 0 Td 62.654321 Tz (was ) Tj 1.4 0 Td 51.851852 Tz (included ) Tj 2.741667 0 Td 50.231481 Tz (in ) Tj 51.428571 0 0 51.428571 0 1542.909988 Tm 6.358333 0 Td 55.092593 Tz (each ) Tj 1.555556 0 Td 51.851852 Tz (formulation ) Tj 3.694444 0 Td 43.75 Tz (to ) Tj 0.797222 0 Td 53.472222 Tz (induce ) Tj 2.119444 0 Td 48.611111 Tz (curing. ) Tj 2.294444 0 Td 72.916667 Tz (hCPR ) Tj 1.983333 0 Td 60.493827 Tz (was ) Tj 1.361111 0 Td 51.311728 Tz (incorporated ) Tj 51.428571 0 0 51.428571 0 1500.294404 Tm 6.377778 0 Td 54.282407 Tz (such ) Tj 1.477778 0 Td 45.37037 Tz (that ) Tj 1.244444 0 Td 32.407407 Tz (it ) Tj 0.525 0 Td 54.73251 Tz (accounted ) Tj 3.111111 0 Td 46.450617 Tz (for ) Tj 0.972222 0 Td 50.231481 Tz (half ) Tj 1.283333 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 49.691358 Tz (the ) Tj 1.011111 0 Td 53.877315 Tz (hydrogel ) Tj 2.780556 0 Td 59.41358 Tz (volume ) Tj 2.275 0 Td 46.990741 Tz (in ) Tj 0.7 0 Td 55.902778 Tz (both ) Tj 51.428571 0 0 51.428571 0 1459.998802 Tm 6.358333 0 Td 52.391975 Tz (experimental ) Tj 3.966667 0 Td 51.311728 Tz (formulations ) Tj 3.85 0 Td 59.41358 Tz (and ) Tj 1.244444 0 Td 51.851852 Tz (served ) Tj 2.022222 0 Td 51.851852 Tz (as ) Tj 0.777778 0 Td 55.092593 Tz (a ) Tj 0.505556 0 Td 52.391975 Tz (source ) Tj 2.022222 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 55.092593 Tz (biologi ) Tj 51.428571 0 0 51.428571 0 1416.811321 Tm 6.358333 0 Td 46.666667 Tz (cally ) Tj 1.594444 0 Td 47.530864 Tz (active ) Tj 1.925 0 Td 51.203704 Tz (molecules. ) Tj 51.428571 0 0 51.428571 0 1377.962716 Tm 7.077778 0 Td 72.916667 Tz (hCPR ) Tj 2.041667 0 Td 52.175926 Tz (processing ) Tj 3.441667 0 Td 42.939815 Tz (lots ) Tj 1.361111 0 Td 58.333333 Tz (were ) Tj 1.691667 0 Td 52.662037 Tz (acquired ) Tj 2.838889 0 Td 52.5 Tz (using ) Tj 1.866667 0 Td 56.712963 Tz (an ) Tj 1.011111 0 Td 52.256944 Tz (in-house ) Tj 51.428571 0 0 51.428571 0 1335.445115 Tm 6.358333 0 Td 47.727273 Tz (centrifugal ) Tj 3.383333 0 Td 57.449495 Tz (methodology ) Tj 4.005556 0 Td 55.740741 Tz (under ) Tj 1.847222 0 Td 56.712963 Tz (an ) Tj 0.913889 0 Td 44.781145 Tz (Institution ) Tj 3.208333 0 Td 58.873457 Tz (Review ) Tj 2.391667 0 Td 58.333333 Tz (Board ) Tj 51.428571 0 0 51.428571 0 1294.45 Tm 6.358333 0 Td 56.30787 Tz (approved ) Tj 2.916667 0 Td 50.636574 Tz (protocol ) Tj 2.663889 0 Td 58.333333 Tz (and ) Tj 1.283333 0 Td 50.771605 Tz (consisted ) Tj 2.916667 0 Td 59.953704 Tz (of ) Tj 0.855556 0 Td 52.5 Tz (pools ) Tj 1.808333 0 Td 58.333333 Tz (from ) Tj 1.672222 0 Td 51.041667 Tz (four ) Tj 1.438889 0 Td 51.851852 Tz (or ) Tj 0.836111 0 Td 45.37037 Tz (five ) Tj 51.428571 0 0 51.428571 0 1253.798088 Tm 6.358333 0 Td 47.530864 Tz (participants ) Tj 3.791667 0 Td 51.851852 Tz (draws. ) Tj 2.080556 0 Td 51.851852 Tz (Processing ) Tj 3.266667 0 Td 61.574074 Tz (was ) Tj 1.263889 0 Td 55.452675 Tz (conducted ) Tj 3.130556 0 Td 45.37037 Tz (in ) Tj 0.7 0 Td 59.773663 Tz (polypropy ) Tj 40 0 0 40 0 1216 Tm 8.175 0 Td 65.625 Tz (lene ) Tj 1.95 0 Td 61.742424 Tz (containers. ) Tj 4.575 0 Td 78.125 Tz (~120 ) Tj 2.3 0 Td 79.166667 Tz (ml ) Tj 1.4 0 Td 70.833333 Tz (of ) Tj 1.125 0 Td 72.5 Tz (blood ) Tj 2.575 0 Td 79.166667 Tz (was ) Tj 1.875 0 Td 75.833333 Tz (drawn ) Tj 2.725 0 Td 73.958333 Tz (from ) Tj 2.225 0 Td 71.875 Tz (each ) Tj 51.428571 0 0 51.428571 0 1166 Tm 6.358333 0 Td 48.611111 Tz (individual ) Tj 3.208333 0 Td 46.180556 Tz (into ) Tj 1.361111 0 Td 51.851852 Tz (acid ) Tj 1.497222 0 Td 43.518519 Tz (citrate ) Tj 2.080556 0 Td 51.041667 Tz (dextrose ) Tj 2.702778 0 Td 54.62963 Tz (formula ) Tj 2.527778 0 Td 87.5 Tz (A ) Tj 0.797222 0 Td 66.111111 Tz (\(ACD\) ) Tj 2.216667 0 Td 61.574074 Tz (and ) Tj 51.428571 0 0 51.428571 0 1128.93401 Tm 6.377778 0 Td 50.771605 Tz (separated ) Tj 2.936111 0 Td 45.37037 Tz (in ) Tj 0.719444 0 Td 63.194444 Tz (30 ) Tj 0.952778 0 Td 61.574074 Tz (ml ) Tj 0.933333 0 Td 46.090535 Tz (aliquots. ) Tj 2.663889 0 Td 51.446759 Tz (Aliquots ) Tj 2.683333 0 Td 59.143519 Tz (were ) Tj 1.594444 0 Td 56.712963 Tz (spun ) Tj 1.555556 0 Td 43.75 Tz (at ) Tj 0.797222 0 Td 57.253086 Tz (160 ) Tj 1.205556 0 Td 64.814815 Tz (g ) Tj 0.583333 0 Td 47.530864 Tz (for ) Tj 51.428571 0 0 51.428571 0 1084.871698 Tm 6.338889 0 Td 49.691358 Tz (ten ) Tj 1.186111 0 Td 50.636574 Tz (minutes, ) Tj 2.644444 0 Td 60.493827 Tz (and ) Tj 1.283333 0 Td 57.79321 Tz (plasma ) Tj 2.313889 0 Td 59.41358 Tz (and ) Tj 1.283333 0 Td 51.203704 Tz (buffy ) Tj 1.808333 0 Td 49.421296 Tz (coat ) Tj 1.419444 0 Td 59.143519 Tz (were ) Tj 1.633333 0 Td 48.971193 Tz (collected ) Tj 2.838889 0 Td 61.574074 Tz (and ) Tj 51.428571 0 0 51.428571 0 1045.1 Tm 6.377778 0 Td 50.771605 Tz (separated ) Tj 2.936111 0 Td 46.990741 Tz (into ) Tj 1.419444 0 Td 56.712963 Tz (10 ) Tj 0.913889 0 Td 59.953704 Tz (ml ) Tj 0.952778 0 Td 45.37037 Tz (aliquots. ) Tj 2.722222 0 Td 45.010288 Tz (Platelets ) Tj 2.683333 0 Td 58.333333 Tz (were ) Tj 1.613889 0 Td 51.041667 Tz (then ) Tj 1.458333 0 Td 51.49177 Tz (separated ) Tj 51.428571 0 0 51.428571 0 1002.757921 Tm 6.358333 0 Td 58.333333 Tz (from ) Tj 1.652778 0 Td 49.691358 Tz (the ) Tj 1.127778 0 Td 58.333333 Tz (serum ) Tj 2.002778 0 Td 53.703704 Tz (through ) Tj 2.508333 0 Td 47.685185 Tz (further ) Tj 2.236111 0 Td 51.851852 Tz (spinning ) Tj 2.702778 0 Td 46.990741 Tz (at ) Tj 0.777778 0 Td 55.092593 Tz (2,000 ) Tj 1.866667 0 Td 45.37037 Tz (g, ) Tj 0.816667 0 Td 59.62963 Tz (serum ) Tj 51.428571 0 0 51.428571 0 960.127108 Tm 6.338889 0 Td 62.654321 Tz (was ) Tj 1.380556 0 Td 51.311728 Tz (pulled ) Tj 2.080556 0 Td 54.012346 Tz (off ) Tj 1.108333 0 Td 59.953704 Tz (of ) Tj 0.875 0 Td 55.092593 Tz (each ) Tj 1.594444 0 Td 52.662037 Tz (tube ) Tj 1.536111 0 Td 50.925926 Tz (leaving ) Tj 2.391667 0 Td 55.092593 Tz (2 ) Tj 0.641667 0 Td 59.953704 Tz (ml ) Tj 0.991667 0 Td 50.925926 Tz (behind. ) Tj 2.469444 0 Td 44.650206 Tz (Platelets ) Tj 51.428571 0 0 51.428571 0 921.421818 Tm 6.338889 0 Td 59.953704 Tz (were ) Tj 1.633333 0 Td 54.208754 Tz (resuspended ) Tj 3.752778 0 Td 59.41358 Tz (and ) Tj 1.244444 0 Td 50.925926 Tz (pooled. ) Tj 2.333333 0 Td 49.826389 Tz (Clotting ) Tj 2.566667 0 Td 61.574074 Tz (was ) Tj 1.283333 0 Td 52.662037 Tz (then ) Tj 1.477778 0 Td 54.62963 Tz (induced ) Tj 2.45 0 Td 63.194444 Tz (by ) Tj 51.428571 0 0 51.428571 0 875.508906 Tm 6.338889 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 49.826389 Tz (addition ) Tj 2.605556 0 Td 58.333333 Tz (of ) Tj 0.797222 0 Td 61.574074 Tz (50 ) Tj 0.952778 0 Td 40.509259 Tz (ul ) Tj 0.777778 0 Td 68.055556 Tz (10% ) Tj 1.458333 0 Td 53.148148 Tz (\(wfv\) ) Tj 1.769444 0 Td 54.62963 Tz (calcium ) Tj 2.508333 0 Td 50.231481 Tz (chloride ) Tj 2.547222 0 Td 54.012346 Tz (per ) Tj 1.205556 0 Td 45.37037 Tz (1.2 ) Tj 1.05 0 Td 61.574074 Tz (ml ) Tj 51.428571 0 0 51.428571 0 838.485993 Tm 6.358333 0 Td 44.965278 Tz (platelet ) Tj 2.372222 0 Td 50.378788 Tz (Suspension. ) Tj 3.538889 0 Td 50.231481 Tz (Clotting ) Tj 2.605556 0 Td 62.654321 Tz (was ) Tj 1.322222 0 Td 56.532922 Tz (performed ) Tj 3.247222 0 Td 48.611111 Tz (for ) Tj 1.05 0 Td 59.41358 Tz (one ) Tj 1.244444 0 Td 57.523148 Tz (hour ) Tj 1.536111 0 Td 46.990741 Tz (at ) Tj 48.571429 0 0 48.571429 0 794 Tm 6.732353 0 Td 58.333333 Tz (37C., ) Tj 2.223529 0 Td 61.764706 Tz (and ) Tj 1.235294 0 Td 54.901961 Tz (then ) Tj 1.420588 0 Td 53.75817 Tz (the ) Tj 1.070588 0 Td 46.895425 Tz (fibrin ) Tj 1.791176 0 Td 48.039216 Tz (clot ) Tj 1.276471 0 Td 65.196078 Tz (was ) Tj 1.317647 0 Td 63.72549 Tz (removed ) Tj 2.779412 0 Td 61.764706 Tz (from ) Tj 1.626471 0 Td 52.614379 Tz (the ) Tj 1.05 0 Td 50.326797 Tz (releasate ) Tj 51.428571 0 0 51.428571 0 755.277228 Tm 6.338889 0 Td 53.148148 Tz (using ) Tj 1.866667 0 Td 55.092593 Tz (a ) Tj 0.583333 0 Td 58.009259 Tz (hypodermic ) Tj 3.752778 0 Td 52.391975 Tz (needle ) Tj 2.119444 0 Td 48.611111 Tz (to ) Tj 0.836111 0 Td 52.5 Tz (expel ) Tj 1.847222 0 Td 47.170782 Tz (releasate ) Tj 2.8 0 Td 51.58179 Tz (incorporated ) Tj 51.428571 0 0 51.428571 0 710.024911 Tm 6.338889 0 Td 51.851852 Tz (within ) Tj 2.022222 0 Td 58.333333 Tz (weakly ) Tj 2.236111 0 Td 50.87963 Tz (contracted ) Tj 3.169444 0 Td 43.209877 Tz (clots. ) Tj 1.711111 0 Td 72.916667 Tz (hCPR ) Tj 1.905556 0 Td 61.574074 Tz (was ) Tj 1.263889 0 Td 42.592593 Tz (sterile ) Tj 1.925 0 Td 42.939815 Tz (filtered ) Tj 2.177778 0 Td 61.574074 Tz (and ) Tj 51.428571 0 0 51.428571 0 668.430303 Tm 6.377778 0 Td 49.151235 Tz (stored ) Tj 1.944444 0 Td 45.37037 Tz (at ) Tj 0.7 0 Td 62.654321 Tz (-80C. ) Tj 2.469444 0 Td 45.37037 Tz (in ) Tj 0.816667 0 Td 35.648148 Tz (1 ) Tj 0.486111 0 Td 61.574074 Tz (ml ) Tj 0.933333 0 Td 47.800926 Tz (aliquots ) Tj 2.488889 0 Td 43.425926 Tz (until ) Tj 1.497222 0 Td 54.282407 Tz (time ) Tj 1.458333 0 Td 59.953704 Tz (of ) Tj 0.797222 0 Td 47.800926 Tz (use. ) Tj 51.428571 0 0 51.428571 0 629.246819 Tm 7.097222 0 Td 49.691358 Tz (All ) Tj 1.108333 0 Td 55.812757 Tz (specimens ) Tj 3.208333 0 Td 58.333333 Tz (were ) Tj 1.594444 0 Td 65.625 Tz (made ) Tj 1.730556 0 Td 52.5 Tz (using ) Tj 1.730556 0 Td 42.592593 Tz (sterile ) Tj 2.002778 0 Td 48.611111 Tz (material ) Tj 2.488889 0 Td 60.493827 Tz (and ) Tj 1.244444 0 Td 53.796296 Tz (origi ) Tj 51.428571 0 0 51.428571 0 585.412888 Tm 6.358333 0 Td 52.5 Tz (nated ) Tj 1.691667 0 Td 58.333333 Tz (from ) Tj 1.536111 0 Td 49.691358 Tz (the ) Tj 0.991667 0 Td 62.384259 Tz (same ) Tj 1.652778 0 Td 55.092593 Tz (raw ) Tj 1.166667 0 Td 48.611111 Tz (material ) Tj 2.508333 0 Td 40.185185 Tz (lots, ) Tj 1.302778 0 Td 53.292181 Tz (excluding ) Tj 2.994444 0 Td 72.106481 Tz (hCPR ) Tj 1.866667 0 Td 59.62963 Tz (which ) Tj 51.428571 0 0 51.428571 0 543.331613 Tm 6.358333 0 Td 64.00463 Tz (came ) Tj 1.886111 0 Td 57.523148 Tz (from ) Tj 1.75 0 Td 48.611111 Tz (individual ) Tj 3.266667 0 Td 55.092593 Tz (blood ) Tj 1.983333 0 Td 47.638889 Tz (collection ) Tj 3.208333 0 Td 52.256944 Tz (poolings ) Tj 2.858333 0 Td 48.611111 Tz (for ) Tj 1.186111 0 Td 55.902778 Tz (each ) Tj 51.428571 0 0 51.428571 0 505.618654 Tm 6.358333 0 Td 51.851852 Tz (experiment. ) Tj 3.538889 0 Td 64.814815 Tz (The ) Tj 1.322222 0 Td 49.074074 Tz (various ) Tj 2.197222 0 Td 58.333333 Tz (components ) Tj 3.636111 0 Td 59.143519 Tz (were ) Tj 1.555556 0 Td 59.62963 Tz (mixed ) Tj 1.944444 0 Td 43.75 Tz (in ) Tj 0.661111 0 Td 47.890947 Tz (Syringes, ) Tj 51.428571 0 0 51.428571 0 460.360202 Tm 6.358333 0 Td 58.333333 Tz (and ) Tj 1.244444 0 Td 38.888889 Tz (1 ) Tj 0.447222 0 Td 61.574074 Tz (ml ) Tj 0.875 0 Td 53.472222 Tz (of ) Tj 0.719444 0 Td 49.691358 Tz (gel ) Tj 1.030556 0 Td 61.574074 Tz (was ) Tj 1.263889 0 Td 49.331276 Tz (delivered ) Tj 2.8 0 Td 47.530864 Tz (via ) Tj 0.991667 0 Td 51.851852 Tz (a ) Tj 0.525 0 Td 51.851852 Tz (16 ) Tj 0.777778 0 Td 57.685185 Tz (gauge ) Tj 1.886111 0 Td 51.851852 Tz (needle ) Tj 1.983333 0 Td 46.990741 Tz (to ) Tj 0.758333 0 Td 51.388889 Tz (12-well ) Tj 51.428571 0 0 51.428571 0 420.091371 Tm 6.358333 0 Td 48.148148 Tz (culture ) Tj 2.236111 0 Td 46.990741 Tz (plates ) Tj 1.925 0 Td 58.981481 Tz (where ) Tj 1.963889 0 Td 49.691358 Tz (gel ) Tj 1.108333 0 Td 48.611111 Tz (discs ) Tj 1.691667 0 Td 58.333333 Tz (were ) Tj 1.594444 0 Td 54.62963 Tz (allowed ) Tj 2.527778 0 Td 45.37037 Tz (to ) Tj 0.758333 0 Td 52.662037 Tz (cure ) Tj 1.477778 0 Td 47.314815 Tz (prior ) Tj 1.613889 0 Td 46.990741 Tz (to ) Tj 51.428571 0 0 51.428571 0 377.554717 Tm 6.358333 0 Td 55.632716 Tz (adding ) Tj 2.177778 0 Td 64.814815 Tz (medium ) Tj 2.527778 0 Td 59.41358 Tz (and ) Tj 1.263889 0 Td 75.347222 Tz (MSCs ) Tj 2.022222 0 Td 43.75 Tz (to ) Tj 0.738889 0 Td 48.611111 Tz (the ) Tj 1.05 0 Td 46.990741 Tz (wells. ) Tj 51.428571 0 0 51.428571 0 339.747804 Tm 7.097222 0 Td 48.611111 Tz (Cell ) Tj 1.341667 0 Td 46.616809 Tz (proliferation ) Tj 3.830556 0 Td 61.574074 Tz (was ) Tj 1.263889 0 Td 51.851852 Tz (assessed ) Tj 2.663889 0 Td 52.777778 Tz (through ) Tj 2.391667 0 Td 47.530864 Tz (lysate ) Tj 1.886111 0 Td 85.339506 Tz (DNA ) Tj 1.711111 0 Td 68.055556 Tz (quan ) Tj 51.428571 0 0 51.428571 0 294.28 Tm 6.338889 0 Td 40.509259 Tz (tity ) Tj 1.225 0 Td 55.902778 Tz (measures ) Tj 2.936111 0 Td 53.148148 Tz (using ) Tj 1.827778 0 Td 48.611111 Tz (the ) Tj 1.108333 0 Td 70.486111 Tz (QUANT-IT ) Tj 3.597222 0 Td 75.257202 Tz (PICOGREEN ) Tj 4.336111 0 Td 82.314815 Tz (DSDNA ) Tj 51.428571 0 0 51.428571 0 253.917874 Tm 6.377778 0 Td 66.975309 Tz (KIT ) Tj 1.380556 0 Td 49.200337 Tz (\(Invitrogen ) Tj 3.441667 0 Td 54.444444 Tz (Corp; ) Tj 1.847222 0 Td 51.131687 Tz (Carlsbad, ) Tj 2.955556 0 Td 45.37037 Tz (Calif.\) ) Tj 2.061111 0 Td 55.092593 Tz (per ) Tj 1.166667 0 Td 48.611111 Tz (the ) Tj 1.108333 0 Td 58.009259 Tz (manufactur ) Tj 51.428571 0 0 51.428571 0 212.550303 Tm 6.358333 0 Td 45.37037 Tz (er's ) Tj 1.263889 0 Td 45.619658 Tz (instructions. ) Tj 3.772222 0 Td 59.143519 Tz (Specimen ) Tj 3.033333 0 Td 50.231481 Tz (values ) Tj 2.022222 0 Td 58.333333 Tz (were ) Tj 1.555556 0 Td 51.851852 Tz (taken ) Tj 1.730556 0 Td 50.231481 Tz (as ) Tj 0.777778 0 Td 50.771605 Tz (the ) Tj 1.05 0 Td 54.166667 Tz (average ) Tj 58.571429 0 0 58.571429 0 169.540943 Tm 5.582927 0 Td 52.642276 Tz (of ) Tj 0.734146 0 Td 43.631436 Tz (duplicate ) Tj 2.543902 0 Td 46.476965 Tz (measures. ) Tj 2.74878 0 Td 51.219512 Tz (5x10" ) Tj 1.758537 0 Td 66.869919 Tz (MSCs ) Tj 1.77561 0 Td 51.930894 Tz (were ) Tj 1.468293 0 Td 48.373984 Tz (seeded ) Tj 1.946341 0 Td 46.95122 Tz (onto ) Tj 1.34878 0 Td 55.96206 Tz (F1+ ) Tj 30 0 0 30 0 2755 Tm 41.833333 0 Td 72.222222 Tz (5 ) Tj 30 0 0 30 0 2547 Tm 41.666667 0 Td 75 Tz (10 ) Tj 30 0 0 30 0 2339 Tm 41.666667 0 Td 72.222222 Tz (15 ) Tj 30 0 0 30 0 1923 Tm 41.6 0 Td 77.777778 Tz (25 ) Tj 30 0 0 30 0 1716 Tm 41.6 0 Td 80.555556 Tz (30 ) Tj 30 0 0 30 0 1507 Tm 41.6 0 Td 77.777778 Tz (35 ) Tj 30 0 0 30 0 1299 Tm 41.566667 0 Td 83.333333 Tz (40 ) Tj 30 0 0 30 0 1091 Tm 41.566667 0 Td 80.555556 Tz (45 ) Tj 30 0 0 30 0 883 Tm 41.6 0 Td 80.555556 Tz (50 ) Tj 30 0 0 30 0 675 Tm 41.6 0 Td 77.777778 Tz (55 ) Tj 30 0 0 30 0 467 Tm 41.633333 0 Td 77.777778 Tz (60 ) Tj 30 0 0 30 0 259 Tm 41.633333 0 Td 75 Tz (65 ) Tj 51.428571 0 0 51.428571 0 2969 Tm 33.6 0 Td 72.916667 Tz (12 ) Tj 51.428571 0 0 51.428571 0 2914.904294 Tm 25.297222 0 Td 58.333333 Tz (and ) Tj 1.283333 0 Td 63.734568 Tz (F2+ ) Tj 1.361111 0 Td 52.391975 Tz (experimental ) Tj 4.044444 0 Td 49.016204 Tz (material ) Tj 2.586111 0 Td 49.016204 Tz (Surfaces ) Tj 2.586111 0 Td 58.333333 Tz (and ) Tj 1.283333 0 Td 51.041667 Tz (standard ) Tj 2.663889 0 Td 46.450617 Tz (tissue ) Tj 51.428571 0 0 51.428571 0 2877.454971 Tm 25.297222 0 Td 48.148148 Tz (culture ) Tj 2.138889 0 Td 44.907407 Tz (plastic ) Tj 2.041667 0 Td 60.277778 Tz (\(TCP\) ) Tj 1.925 0 Td 48.611111 Tz (for ) Tj 0.991667 0 Td 57.613169 Tz (monolayer ) Tj 3.227778 0 Td 54.012346 Tz (expansion ) Tj 3.052778 0 Td 48.611111 Tz (in ) Tj 0.680556 0 Td 51.851852 Tz (polystyrene ) Tj 51.428571 0 0 51.428571 0 2833.448931 Tm 25.277778 0 Td 46.990741 Tz (tissue ) Tj 1.827778 0 Td 48.148148 Tz (culture ) Tj 2.177778 0 Td 46.990741 Tz (plates ) Tj 1.866667 0 Td 51.49177 Tz (\(Corning; ) Tj 2.936111 0 Td 53.877315 Tz (Corning, ) Tj 2.761111 0 Td 47.530864 Tz (N.Y.\). ) Tj 1.866667 0 Td 51.446759 Tz (Cultures ) Tj 2.644444 0 Td 59.143519 Tz (were ) Tj 51.428571 0 0 51.428571 0 2789.686747 Tm 25.297222 0 Td 54.62963 Tz (allowed ) Tj 2.411111 0 Td 45.37037 Tz (to ) Tj 0.680556 0 Td 58.333333 Tz (expand ) Tj 2.255556 0 Td 47.530864 Tz (for ) Tj 0.972222 0 Td 46.666667 Tz (Zero, ) Tj 1.516667 0 Td 45.910494 Tz (three, ) Tj 1.769444 0 Td 49.691358 Tz (seven, ) Tj 1.925 0 Td 59.41358 Tz (and ) Tj 1.205556 0 Td 49.826389 Tz (fourteen ) Tj 2.566667 0 Td 54.282407 Tz (days ) Tj 1.438889 0 Td 61.574074 Tz (on ) Tj 51.428571 0 0 51.428571 0 2747.979798 Tm 25.277778 0 Td 50.771605 Tz (the ) Tj 1.225 0 Td 49.421296 Tz (material ) Tj 2.683333 0 Td 49.016204 Tz (Surfaces ) Tj 2.644444 0 Td 60.493827 Tz (and ) Tj 1.380556 0 Td 49.691358 Tz (the ) Tj 1.205556 0 Td 54.012346 Tz (manufacturer ) Tj 4.141667 0 Td 47.685185 Tz (treated ) Tj 2.313889 0 Td 44.907407 Tz (plastic ) Tj 40 0 0 40 0 2713 Tm 32.55 0 Td 60.416667 Tz (Surface. ) Tj 3.2 0 Td 65.625 Tz (Cultures ) Tj 3.475 0 Td 76.041667 Tz (were ) Tj 2.175 0 Td 68.333333 Tz (maintained ) Tj 4.425 0 Td 71.666667 Tz (under ) Tj 2.425 0 Td 63.888889 Tz (the ) Tj 1.45 0 Td 81.25 Tz (same ) Tj 2.25 0 Td 84.722222 Tz (medium ) Tj 48.571429 0 0 48.571429 0 2666 Tm 26.785294 0 Td 61.764706 Tz (and ) Tj 1.358824 0 Td 56.749623 Tz (environmental ) Tj 4.714706 0 Td 53.186275 Tz (conditions ) Tj 3.5 0 Td 54.520697 Tz (described ) Tj 3.191176 0 Td 56.045752 Tz (above, ) Tj 2.285294 0 Td 62.908497 Tz (and ) Tj 1.420588 0 Td 51.470588 Tz (2.2 ) Tj 1.235294 0 Td 65.196078 Tz (ml ) Tj 51.428571 0 0 51.428571 0 2624.021871 Tm 25.297222 0 Td 65.354938 Tz (medium ) Tj 2.527778 0 Td 54.012346 Tz (per ) Tj 1.108333 0 Td 51.041667 Tz (well ) Tj 1.380556 0 Td 61.574074 Tz (was ) Tj 1.283333 0 Td 50.05144 Tz (refreshed ) Tj 2.819444 0 Td 53.148148 Tz (every ) Tj 1.75 0 Td 48.611111 Tz (three ) Tj 1.594444 0 Td 53.472222 Tz (or ) Tj 0.758333 0 Td 51.851852 Tz (four ) Tj 1.380556 0 Td 49.259259 Tz (days. ) Tj 1.633333 0 Td 51.041667 Tz (Cell ) Tj 51.428571 0 0 51.428571 0 2582.91239 Tm 25.297222 0 Td 46.759259 Tz (lysates ) Tj 2.236111 0 Td 56.712963 Tz (from ) Tj 1.613889 0 Td 55.092593 Tz (each ) Tj 1.575 0 Td 50.771605 Tz (condition ) Tj 2.994444 0 Td 48.251029 Tz (\(material ) Tj 2.838889 0 Td 59.41358 Tz (and ) Tj 1.283333 0 Td 54.282407 Tz (time ) Tj 1.516667 0 Td 48.611111 Tz (point\) ) Tj 2.002778 0 Td 59.143519 Tz (were ) Tj 58.571429 0 0 58.571429 0 2540.709559 Tm 22.212195 0 Td 42.999097 Tz (collected ) Tj 2.492683 0 Td 44.105691 Tz (\(n-3\); ) Tj 1.77561 0 Td 45.528455 Tz (nine ) Tj 1.263415 0 Td 52.357724 Tz (5x10" ) Tj 1.741463 0 Td 73.98374 Tz (MSC ) Tj 1.519512 0 Td 49.006323 Tz (specimens ) Tj 2.817073 0 Td 52.642276 Tz (were ) Tj 1.45122 0 Td 45.528455 Tz (taken ) Tj 1.553659 0 Td 41.734417 Tz (for ) Tj 51.428571 0 0 51.428571 0 2501.11976 Tm 25.277778 0 Td 55.092593 Tz (time ) Tj 1.477778 0 Td 47.314815 Tz (Zero, ) Tj 1.575 0 Td 53.06713 Tz (allowing ) Tj 2.702778 0 Td 48.611111 Tz (for ) Tj 0.991667 0 Td 58.333333 Tz (a ) Tj 0.486111 0 Td 54.282407 Tz (balanced ) Tj 2.761111 0 Td 52.391975 Tz (experimental ) Tj 3.966667 0 Td 48.611111 Tz (design. ) Tj 2.216667 0 Td 72.376543 Tz (Col ) Tj 51.428571 0 0 51.428571 0 2457.354446 Tm 25.297222 0 Td 46.759259 Tz (lection ) Tj 2.119444 0 Td 50.05144 Tz (protocols ) Tj 2.877778 0 Td 49.691358 Tz (varied ) Tj 1.925 0 Td 58.333333 Tz (due ) Tj 1.205556 0 Td 45.37037 Tz (to ) Tj 0.719444 0 Td 48.148148 Tz (release ) Tj 2.138889 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 44.722222 Tz (cells ) Tj 1.477778 0 Td 57.523148 Tz (from ) Tj 1.575 0 Td 48.611111 Tz (the ) Tj 1.05 0 Td 67.24537 Tz (mate ) Tj 51.428571 0 0 51.428571 0 2417.09988 Tm 25.297222 0 Td 41.481481 Tz (rials ) Tj 1.4 0 Td 59.953704 Tz (becoming ) Tj 3.072222 0 Td 60.763889 Tz (more ) Tj 1.633333 0 Td 51.262626 Tz (challenging ) Tj 3.519444 0 Td 53.472222 Tz (with ) Tj 1.458333 0 Td 54.282407 Tz (time ) Tj 1.438889 0 Td 46.990741 Tz (in ) Tj 0.719444 0 Td 45.37037 Tz (culture. ) Tj 2.372222 0 Td 62.222222 Tz (Media ) Tj 51.428571 0 0 51.428571 0 2375.828087 Tm 25.277778 0 Td 62.654321 Tz (was ) Tj 1.302778 0 Td 38.240741 Tz (first ) Tj 1.283333 0 Td 48.971193 Tz (aspirated ) Tj 2.8 0 Td 58.333333 Tz (from ) Tj 1.575 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 50.555556 Tz (wells ) Tj 1.672222 0 Td 59.41358 Tz (and ) Tj 1.225 0 Td 56.17284 Tz (specimens ) Tj 3.208333 0 Td 59.143519 Tz (were ) Tj 1.575 0 Td 49.691358 Tz (rinsed ) Tj 51.428571 0 0 51.428571 0 2331.873449 Tm 25.277778 0 Td 54.282407 Tz (with ) Tj 1.575 0 Td 65.462963 Tz (HBSS. ) Tj 2.216667 0 Td 74.537037 Tz (MSCs ) Tj 2.002778 0 Td 59.953704 Tz (were ) Tj 1.633333 0 Td 47.530864 Tz (easily ) Tj 1.944444 0 Td 48.971193 Tz (collected ) Tj 2.858333 0 Td 58.333333 Tz (from ) Tj 1.613889 0 Td 75.617284 Tz (TCP ) Tj 1.575 0 Td 49.074074 Tz (control ) Tj 51.428571 0 0 51.428571 0 2295.022333 Tm 25.277778 0 Td 51.203704 Tz (wells ) Tj 1.827778 0 Td 46.450617 Tz (via ) Tj 1.108333 0 Td 54.282407 Tz (mild ) Tj 1.555556 0 Td 47.222222 Tz (trypsinization ) Tj 4.2 0 Td 46.990741 Tz (in ) Tj 0.797222 0 Td 48.611111 Tz (the ) Tj 1.088889 0 Td 53.877315 Tz (presence ) Tj 2.8 0 Td 59.953704 Tz (of ) Tj 0.816667 0 Td 54.768519 Tz (ethylenedi ) Tj 51.428571 0 0 51.428571 0 2248 Tm 25.297222 0 Td 49.623843 Tz (aminetetraacetic ) Tj 5.094444 0 Td 51.041667 Tz (acid ) Tj 1.575 0 Td 59.259259 Tz (\(EDTA\). ) Tj 2.819444 0 Td 51.203704 Tz (Cells ) Tj 1.847222 0 Td 59.953704 Tz (were ) Tj 1.75 0 Td 48.971193 Tz (collected ) Tj 2.975 0 Td 59.143519 Tz (from ) Tj 51.428571 0 0 51.428571 0 2210.388398 Tm 25.297222 0 Td 52.391975 Tz (experimental ) Tj 4.219444 0 Td 48.611111 Tz (materials ) Tj 3.013889 0 Td 54.166667 Tz (through ) Tj 2.702778 0 Td 50.231481 Tz (multiple ) Tj 2.8 0 Td 50.555556 Tz (treatments ) Tj 3.441667 0 Td 54.282407 Tz (with ) Tj 51.428571 0 0 51.428571 0 2168.838384 Tm 25.297222 0 Td 53.240741 Tz (varying ) Tj 2.566667 0 Td 50.462963 Tz (concentrations ) Tj 4.627778 0 Td 55.092593 Tz (of ) Tj 0.913889 0 Td 46.180556 Tz (trypsin, ) Tj 2.605556 0 Td 53.148148 Tz (never ) Tj 1.944444 0 Td 54.372428 Tz (exceeding ) Tj 3.286111 0 Td 63.194444 Tz (2.5% ) Tj 51.428571 0 0 51.428571 0 2124.3 Tm 25.316667 0 Td 48.070988 Tz (\(w/v\), ) Tj 1.983333 0 Td 45.37037 Tz (in ) Tj 0.777778 0 Td 49.691358 Tz (the ) Tj 1.108333 0 Td 55.555556 Tz (absence ) Tj 2.547222 0 Td 59.953704 Tz (of ) Tj 0.894444 0 Td 66.759259 Tz (EDTA, ) Tj 2.236111 0 Td 52.662037 Tz (avoiding ) Tj 2.741667 0 Td 48.316498 Tz (dissolution ) Tj 3.422222 0 Td 59.953704 Tz (of ) Tj 0.836111 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 2085.46634 Tm 25.277778 0 Td 50.555556 Tz (hydrogels. ) Tj 3.266667 0 Td 51.49177 Tz (Collected ) Tj 2.975 0 Td 44.074074 Tz (cells ) Tj 1.555556 0 Td 58.333333 Tz (were ) Tj 1.594444 0 Td 47.800926 Tz (pelleted ) Tj 2.469444 0 Td 60.493827 Tz (and ) Tj 1.263889 0 Td 51.851852 Tz (lysed ) Tj 1.730556 0 Td 46.990741 Tz (in ) Tj 0.777778 0 Td 61.574074 Tz (deion ) Tj 51.428571 0 0 51.428571 0 2041.980676 Tm 25.297222 0 Td 51.041667 Tz (ized ) Tj 1.341667 0 Td 48.070988 Tz (water. ) Tj 1.905556 0 Td 51.388889 Tz (Lysates ) Tj 2.313889 0 Td 58.333333 Tz (were ) Tj 1.536111 0 Td 49.151235 Tz (stored ) Tj 1.886111 0 Td 46.990741 Tz (at ) Tj 0.7 0 Td 60.493827 Tz (-80C. ) Tj 2.333333 0 Td 44.074074 Tz (until ) Tj 1.477778 0 Td 53.472222 Tz (time ) Tj 1.419444 0 Td 53.472222 Tz (of ) Tj 0.719444 0 Td 60.925926 Tz (analy ) Tj 27.142857 0 0 27.142857 0 2007 Tm 47.968421 0 Td 70.614035 Tz (S1S. ) Tj 51.428571 0 0 51.428571 0 1957.834783 Tm 26.016667 0 Td 85.339506 Tz (MSC ) Tj 1.769444 0 Td 45.802469 Tz (differentiation ) Tj 4.316667 0 Td 54.444444 Tz (along ) Tj 1.847222 0 Td 52.10114 Tz (osteochondral ) Tj 4.277778 0 Td 58.333333 Tz (and ) Tj 1.283333 0 Td 45.100309 Tz (fibroblastic ) Tj 51.428571 0 0 51.428571 0 1920.588785 Tm 25.297222 0 Td 49.421296 Tz (lineages ) Tj 2.488889 0 Td 62.654321 Tz (was ) Tj 1.263889 0 Td 49.537037 Tz (studied ) Tj 2.177778 0 Td 53.703704 Tz (through ) Tj 2.372222 0 Td 58.333333 Tz (gene ) Tj 1.516667 0 Td 51.527778 Tz (expression ) Tj 3.188889 0 Td 52.256944 Tz (changes. ) Tj 2.644444 0 Td 75.347222 Tz (Quan ) Tj 51.428571 0 0 51.428571 0 1878.176744 Tm 25.277778 0 Td 42.12963 Tz (titative ) Tj 2.158333 0 Td 49.074074 Tz (reverse ) Tj 2.177778 0 Td 47.364672 Tz (transcription ) Tj 3.791667 0 Td 56.388889 Tz (polymerase ) Tj 3.519444 0 Td 51.851852 Tz (chain ) Tj 1.672222 0 Td 49.016204 Tz (reaction ) Tj 2.508333 0 Td 68.865741 Tz (\(qPT ) Tj 51.428571 0 0 51.428571 0 1834 Tm 25.316667 0 Td 67.24537 Tz (PCR\) ) Tj 1.788889 0 Td 61.574074 Tz (was ) Tj 1.302778 0 Td 55.092593 Tz (used ) Tj 1.516667 0 Td 45.37037 Tz (to ) Tj 0.738889 0 Td 54.012346 Tz (determine ) Tj 3.111111 0 Td 48.611111 Tz (the ) Tj 1.088889 0 Td 46.666667 Tz (materials ) Tj 3.033333 0 Td 44.444444 Tz (ability ) Tj 2.061111 0 Td 45.37037 Tz (to ) Tj 0.758333 0 Td 68.055556 Tz (tempo ) Tj 51.428571 0 0 51.428571 0 1793.662906 Tm 25.297222 0 Td 44.074074 Tz (rally ) Tj 1.963889 0 Td 55.902778 Tz (modulate ) Tj 3.325 0 Td 85.069444 Tz (mRNA ) Tj 2.644444 0 Td 49.494949 Tz (expression. ) Tj 3.869444 0 Td 54.282407 Tz (This ) Tj 1.905556 0 Td 55.092593 Tz (allowed ) Tj 2.936111 0 Td 47.530864 Tz (for ) Tj 51.428571 0 0 51.428571 0 1752.505402 Tm 25.297222 0 Td 46.254209 Tz (distinction ) Tj 3.286111 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 50.771605 Tz (the ) Tj 1.127778 0 Td 50 Tz (various ) Tj 2.294444 0 Td 52.662037 Tz (chondrocytic ) Tj 3.986111 0 Td 55.092593 Tz (phenotypes ) Tj 3.538889 0 Td 56.712963 Tz (of ) Tj 0.797222 0 Td 68.865741 Tz (endo ) Tj 57.142857 0 0 57.142857 0 1709.224971 Tm 22.7675 0 Td 47.395833 Tz (chondral ) Tj 2.415 0 Td 41.5625 Tz (ossification ) Tj 3.08 0 Td 54.444444 Tz (and ) Tj 1.085 0 Td 41.157407 Tz (articular ) Tj 2.2925 0 Td 42.12963 Tz (cartilage ) Tj 2.38 0 Td 45.791667 Tz (formation. ) Tj 2.8875 0 Td 56.875 Tz (5x10 ) Tj 51.428571 0 0 51.428571 0 1666 Tm 25.297222 0 Td 75.347222 Tz (MSCs ) Tj 1.983333 0 Td 59.143519 Tz (were ) Tj 1.594444 0 Td 55.092593 Tz (seeded ) Tj 2.158333 0 Td 53.472222 Tz (onto ) Tj 1.477778 0 Td 55.092593 Tz (F1+, ) Tj 1.536111 0 Td 54.282407 Tz (F2+, ) Tj 1.477778 0 Td 53.472222 Tz (or ) Tj 0.797222 0 Td 48.148148 Tz (control ) Tj 2.255556 0 Td 49.016204 Tz (material ) Tj 2.527778 0 Td 55.092593 Tz (\(F1-\) ) Tj 52.857143 0 0 52.857143 0 1623.842615 Tm 24.613514 0 Td 44.144144 Tz (in ) Tj 0.7 0 Td 46.846847 Tz (culture ) Tj 2.118919 0 Td 45.720721 Tz (plates ) Tj 1.816216 0 Td 56.756757 Tz (and ) Tj 1.191892 0 Td 52.027027 Tz (maintained ) Tj 3.272973 0 Td 54.864865 Tz (under ) Tj 1.759459 0 Td 49.399399 Tz (the ) Tj 1.040541 0 Td 60.698198 Tz (same ) Tj 1.608108 0 Td 64.114114 Tz (medium ) Tj 2.459459 0 Td 57.807808 Tz (and ) Tj 40 0 0 40 0 1590 Tm 32.525 0 Td 68.589744 Tz (environmental ) Tj 5.525 0 Td 65 Tz (conditions ) Tj 4.075 0 Td 66.666667 Tz (described ) Tj 3.75 0 Td 66.666667 Tz (above. ) Tj 2.625 0 Td 83.333333 Tz (F ) Tj 0.625 0 Td 41.666667 Tz (1 ) Tj 0.375 0 Td 79.166667 Tz (- ) Tj 0.65 0 Td 79.166667 Tz (was ) Tj 1.625 0 Td 59.259259 Tz (identical ) Tj 51.428571 0 0 51.428571 0 1542.882427 Tm 25.277778 0 Td 45.37037 Tz (to ) Tj 0.855556 0 Td 63.734568 Tz (F1+ ) Tj 1.380556 0 Td 52.662037 Tz (with ) Tj 1.516667 0 Td 49.691358 Tz (the ) Tj 1.108333 0 Td 52.572016 Tz (exception ) Tj 3.072222 0 Td 56.712963 Tz (of ) Tj 0.855556 0 Td 42.12963 Tz (sterile ) Tj 2.002778 0 Td 54.444444 Tz (water ) Tj 1.847222 0 Td 50.05144 Tz (replacing ) Tj 2.916667 0 Td 63.518519 Tz (hCPR, ) Tj 51.428571 0 0 51.428571 0 1504.422434 Tm 25.297222 0 Td 53.148148 Tz (accounting ) Tj 3.344444 0 Td 48.611111 Tz (for ) Tj 1.011111 0 Td 67.24537 Tz (100% ) Tj 1.75 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 48.611111 Tz (the ) Tj 0.991667 0 Td 48.611111 Tz (gel ) Tj 1.030556 0 Td 46.990741 Tz (liquid ) Tj 1.808333 0 Td 56.388889 Tz (component. ) Tj 3.519444 0 Td 50.231481 Tz (Cell ) Tj 1.341667 0 Td 45.37037 Tz (pellets ) Tj 51.428571 0 0 51.428571 0 1457 Tm 25.297222 0 Td 58.333333 Tz (from ) Tj 1.613889 0 Td 55.902778 Tz (each ) Tj 1.536111 0 Td 49.421296 Tz (material ) Tj 2.586111 0 Td 53.796296 Tz (\(n=2\) ) Tj 1.788889 0 Td 59.143519 Tz (were ) Tj 1.594444 0 Td 49.331276 Tz (collected ) Tj 2.838889 0 Td 50.231481 Tz (as ) Tj 0.816667 0 Td 51.49177 Tz (described ) Tj 2.975 0 Td 57.685185 Tz (above ) Tj 51.428571 0 0 51.428571 0 1415.680203 Tm 25.297222 0 Td 45.37037 Tz (after ) Tj 1.536111 0 Td 45.37037 Tz (five ) Tj 1.263889 0 Td 49.691358 Tz (hours, ) Tj 2.041667 0 Td 51.041667 Tz (one, ) Tj 1.419444 0 Td 45.37037 Tz (three, ) Tj 1.847222 0 Td 50.231481 Tz (seven, ) Tj 2.002778 0 Td 59.41358 Tz (and ) Tj 1.302778 0 Td 49.826389 Tz (fourteen ) Tj 2.625 0 Td 54.282407 Tz (days ) Tj 1.536111 0 Td 45.37037 Tz (in ) Tj 0.777778 0 Td 61.574074 Tz (cul ) Tj 58.571429 0 0 58.571429 0 1376.800965 Tm 22.195122 0 Td 38.130081 Tz (ture. ) Tj 1.297561 0 Td 75.880759 Tz (MSC ) Tj 1.468293 0 Td 40.243902 Tz (pellets ) Tj 1.826829 0 Td 47.235772 Tz (\(n=2\) ) Tj 1.519512 0 Td 45.243902 Tz (containing ) Tj 2.85122 0 Td 54.776423 Tz (5x10 ) Tj 1.656098 0 Td 39.837398 Tz (cells ) Tj 1.314634 0 Td 51.930894 Tz (were ) Tj 1.365854 0 Td 43.038618 Tz (retained ) Tj 51.428571 0 0 51.428571 0 1332.762914 Tm 25.297222 0 Td 45.37037 Tz (at ) Tj 0.855556 0 Td 54.282407 Tz (time ) Tj 1.594444 0 Td 51.041667 Tz (zero ) Tj 1.555556 0 Td 42.12963 Tz (to ) Tj 0.855556 0 Td 50.555556 Tz (serve ) Tj 1.827778 0 Td 48.611111 Tz (as ) Tj 0.933333 0 Td 48.611111 Tz (the ) Tj 1.166667 0 Td 48.408565 Tz (undifferentiated ) Tj 4.919444 0 Td 49.537037 Tz (control ) Tj 2.372222 0 Td 58.333333 Tz (gene ) Tj 51.428571 0 0 51.428571 0 1293.516206 Tm 25.297222 0 Td 49.494949 Tz (expression. ) Tj 3.441667 0 Td 45.37037 Tz (Pellets ) Tj 2.061111 0 Td 59.143519 Tz (were ) Tj 1.555556 0 Td 49.151235 Tz (stored ) Tj 1.905556 0 Td 45.37037 Tz (at ) Tj 0.661111 0 Td 61.033951 Tz (-80C. ) Tj 2.372222 0 Td 44.074074 Tz (until ) Tj 1.497222 0 Td 53.472222 Tz (time ) Tj 1.4 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 60.925926 Tz (analy ) Tj 51.428571 0 0 51.428571 0 1249 Tm 25.316667 0 Td 38.078704 Tz (sis. ) Tj 1.225 0 Td 71.794872 Tz (SABIOSCIENCES ) Tj 5.930556 0 Td 57.79321 Tz (\(Germantown, ) Tj 4.472222 0 Td 59.953704 Tz (Md.\) ) Tj 1.711111 0 Td 59.41358 Tz (and ) Tj 1.341667 0 Td 77.237654 Tz (QIAGEN ) Tj 51.428571 0 0 51.428571 0 1209.723537 Tm 25.316667 0 Td 58.063272 Tz (\(Germantown, ) Tj 4.452778 0 Td 59.143519 Tz (Md.\) ) Tj 1.711111 0 Td 49.826389 Tz (reagents ) Tj 2.663889 0 Td 59.143519 Tz (were ) Tj 1.691667 0 Td 55.092593 Tz (used ) Tj 1.575 0 Td 54.012346 Tz (per ) Tj 1.225 0 Td 54.282407 Tz (manufactures ) Tj 51.428571 0 0 51.428571 0 1168.727044 Tm 25.297222 0 Td 46.990741 Tz (instructions ) Tj 3.675 0 Td 45.37037 Tz (to ) Tj 0.816667 0 Td 55.092593 Tz (perform ) Tj 2.605556 0 Td 61.574074 Tz (qRT-PCR. ) Tj 3.286111 0 Td 82.098765 Tz (RNA ) Tj 1.75 0 Td 61.574074 Tz (was ) Tj 1.4 0 Td 46.990741 Tz (isolated ) Tj 2.527778 0 Td 59.143519 Tz (from ) Tj 51.428571 0 0 51.428571 0 1126.88 Tm 25.297222 0 Td 43.75 Tz (cell ) Tj 1.225 0 Td 44.444444 Tz (pellets ) Tj 2.080556 0 Td 52.5 Tz (using ) Tj 1.75 0 Td 48.611111 Tz (the ) Tj 1.069444 0 Td 80.478395 Tz (RNEASY ) Tj 3.033333 0 Td 68.055556 Tz (MINI ) Tj 1.847222 0 Td 68.055556 Tz (KIT ) Tj 1.380556 0 Td 55.555556 Tz (\(Qiagen ) Tj 2.488889 0 Td 71.83642 Tz (German ) Tj 51.428571 0 0 51.428571 0 1085.98708 Tm 25.277778 0 Td 54.444444 Tz (town, ) Tj 1.944444 0 Td 52.5 Tz (Md.\). ) Tj 1.866667 0 Td 47.638889 Tz (Typically, ) Tj 3.188889 0 Td 51.851852 Tz (recovered ) Tj 3.111111 0 Td 82.098765 Tz (RNA ) Tj 1.788889 0 Td 60.493827 Tz (was ) Tj 1.380556 0 Td 51.131687 Tz (subjected ) Tj 3.052778 0 Td 43.75 Tz (to ) Tj 0.816667 0 Td 55.092593 Tz (a ) Tj 51.428571 0 0 51.428571 0 1042.224787 Tm 25.316667 0 Td 55.632716 Tz (second ) Tj 2.138889 0 Td 52.662037 Tz (pass ) Tj 1.438889 0 Td 54.282407 Tz (over ) Tj 1.458333 0 Td 63.734568 Tz (new ) Tj 1.361111 0 Td 49.421296 Tz (spin ) Tj 1.341667 0 Td 58.796296 Tz (columns ) Tj 2.663889 0 Td 45.37037 Tz (to ) Tj 0.758333 0 Td 59.41358 Tz (remove ) Tj 2.333333 0 Td 54.822531 Tz (PCR-interfer ) Tj 51.428571 0 0 51.428571 0 1002.389486 Tm 25.297222 0 Td 50.771605 Tz (ing ) Tj 1.108333 0 Td 51.851852 Tz (contaminants, ) Tj 4.238889 0 Td 58.333333 Tz (which ) Tj 1.925 0 Td 59.143519 Tz (were ) Tj 1.575 0 Td 45.37037 Tz (likely ) Tj 1.808333 0 Td 48.206019 Tz (alginate ) Tj 2.488889 0 Td 52.391975 Tz (and/or ) Tj 2.022222 0 Td 55.092593 Tz (calcium ) Tj 51.428571 0 0 51.428571 0 958.507463 Tm 25.297222 0 Td 50.231481 Tz (ions ) Tj 1.497222 0 Td 55.092593 Tz (per ) Tj 1.263889 0 Td 50.356125 Tz (conversations ) Tj 4.258333 0 Td 53.472222 Tz (with ) Tj 1.594444 0 Td 50.771605 Tz (the ) Tj 1.225 0 Td 50.854701 Tz (manufacturer. ) Tj 4.297222 0 Td 61.934156 Tz (On-column ) Tj 51.428571 0 0 51.428571 0 920.938607 Tm 25.297222 0 Td 51.557239 Tz (degradation ) Tj 3.538889 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 85.339506 Tz (DNA ) Tj 1.633333 0 Td 62.654321 Tz (was ) Tj 1.263889 0 Td 55.092593 Tz (conducted ) Tj 3.111111 0 Td 52.391975 Tz (during ) Tj 1.963889 0 Td 50.771605 Tz (the ) Tj 1.030556 0 Td 38.240741 Tz (first ) Tj 1.244444 0 Td 51.851852 Tz (pass ) Tj 1.361111 0 Td 52.5 Tz (using ) Tj 51.428571 0 0 51.428571 0 876.22449 Tm 25.277778 0 Td 50.771605 Tz (the ) Tj 1.361111 0 Td 72.916667 Tz (RNASE-FREE ) Tj 4.841667 0 Td 79.074074 Tz (DNASE ) Tj 2.8 0 Td 72.376543 Tz (SET ) Tj 1.75 0 Td 55.555556 Tz (\(Qiagen ) Tj 2.761111 0 Td 59.806397 Tz (Germantown, ) Tj 51.428571 0 0 51.428571 0 833 Tm 25.297222 0 Td 53.148148 Tz (Md.\). ) Tj 57.142857 0 0 57.142857 0 795.547558 Tm 23.415 0 Td 50 Tz (Reverse ) Tj 2.2575 0 Td 42.403846 Tz (transcription ) Tj 3.465 0 Td 55.416667 Tz (was ) Tj 1.155 0 Td 50.555556 Tz (performed ) Tj 2.905 0 Td 48.125 Tz (with ) Tj 1.33 0 Td 44.722222 Tz (the ) Tj 0.9975 0 Td 62.708333 Tz (RT ) Tj 1.19 0 Td 58.916667 Tz (FIRST ) Tj 51.428571 0 0 51.428571 0 750 Tm 25.316667 0 Td 82.314815 Tz (SRAND ) Tj 2.547222 0 Td 79.398148 Tz (cDNA ) Tj 2.022222 0 Td 66.975309 Tz (KIT ) Tj 1.322222 0 Td 55.092593 Tz (\(SABiosciences ) Tj 4.783333 0 Td 59.511785 Tz (Germantown, ) Tj 4.063889 0 Td 59.143519 Tz (Md.\) ) Tj 1.536111 0 Td 61.574074 Tz (on ) Tj 0.836111 0 Td 58.333333 Tz (a ) Tj 51.428571 0 0 51.428571 0 712.005834 Tm 25.297222 0 Td 81.018519 Tz (MASTERCYCLER ) Tj 6.008333 0 Td 71.296296 Tz (EP ) Tj 1.011111 0 Td 77.372685 Tz (GRADIENT ) Tj 3.869444 0 Td 56.388889 Tz (\(Eppendorf ) Tj 3.675 0 Td 59.953704 Tz (Hamburg, ) Tj 51.428571 0 0 51.428571 0 670.498173 Tm 25.316667 0 Td 48.611111 Tz (Del.\) ) Tj 1.652778 0 Td 52.314815 Tz (thermal ) Tj 2.411111 0 Td 45.37037 Tz (cycler. ) Tj 2.177778 0 Td 52.572016 Tz (Real-time ) Tj 3.033333 0 Td 46.527778 Tz (\(quantitative\) ) Tj 4.122222 0 Td 75.617284 Tz (PCR ) Tj 1.555556 0 Td 62.654321 Tz (was ) Tj 1.322222 0 Td 64.814815 Tz (per ) Tj 51.428571 0 0 51.428571 0 627.881773 Tm 25.297222 0 Td 58.333333 Tz (formed ) Tj 2.352778 0 Td 45.37037 Tz (in ) Tj 0.797222 0 Td 58.333333 Tz (custom ) Tj 2.352778 0 Td 45.37037 Tz (built ) Tj 1.575 0 Td 55.632716 Tz (primer ) Tj 2.197222 0 Td 47.530864 Tz (plates ) Tj 1.983333 0 Td 55.092593 Tz (\(SABiosciences ) Tj 4.861111 0 Td 74.537037 Tz (Ger ) Tj 51.428571 0 0 51.428571 0 584 Tm 25.316667 0 Td 58.738426 Tz (mantown, ) Tj 3.091667 0 Td 55.740741 Tz (Md.\)\) ) Tj 1.847222 0 Td 53.472222 Tz (with ) Tj 1.555556 0 Td 78.587963 Tz (SYBR ) Tj 2.1 0 Td 79.722222 Tz (GREEN ) Tj 2.644444 0 Td 82.098765 Tz (MASTER ) Tj 3.188889 0 Td 75.617284 Tz (MIX ) Tj 1.594444 0 Td 78.858025 Tz (\(SA ) Tj 51.428571 0 0 51.428571 0 542 Tm 25.316667 0 Td 52.146465 Tz (Biosciences ) Tj 3.597222 0 Td 60.395623 Tz (Germantown, ) Tj 4.180556 0 Td 55.740741 Tz (Md.\)\) ) Tj 1.808333 0 Td 61.574074 Tz (on ) Tj 0.913889 0 Td 56.712963 Tz (an ) Tj 0.894444 0 Td 73.148148 Tz (ICYCLER ) Tj 3.247222 0 Td 61.574074 Tz (iQ ) Tj 0.933333 0 Td 77.777778 Tz (REAL ) Tj 51.428571 0 0 51.428571 0 501.996337 Tm 25.297222 0 Td 74.537037 Tz (TIME ) Tj 2.002778 0 Td 76.697531 Tz (PCR ) Tj 1.575 0 Td 49.331276 Tz (detection ) Tj 2.897222 0 Td 55.092593 Tz (system ) Tj 2.216667 0 Td 53.652263 Tz (\(Bio-Rad; ) Tj 3.169444 0 Td 50.411523 Tz (Hercules, ) Tj 2.916667 0 Td 45.833333 Tz (Calif.\) ) Tj 2.119444 0 Td 46.990741 Tz (to ) Tj 51.428571 0 0 51.428571 0 461.484108 Tm 25.297222 0 Td 54.012346 Tz (determine ) Tj 3.111111 0 Td 51.851852 Tz (cycle ) Tj 1.808333 0 Td 49.691358 Tz (threshold ) Tj 2.936111 0 Td 47.800926 Tz (\(Ct\) ) Tj 1.4 0 Td 46.759259 Tz (values. ) Tj 2.216667 0 Td 64.814815 Tz (Gene ) Tj 1.769444 0 Td 51.262626 Tz (expressions ) Tj 3.616667 0 Td 58.333333 Tz (of ) Tj 51.428571 0 0 51.428571 0 420.998755 Tm 25.297222 0 Td 44.965278 Tz (interest ) Tj 2.333333 0 Td 51.851852 Tz (are ) Tj 1.108333 0 Td 44.290123 Tz (listed ) Tj 1.808333 0 Td 43.75 Tz (in ) Tj 0.719444 0 Td 54.444444 Tz (Table ) Tj 1.847222 0 Td 40.509259 Tz (2. ) Tj 0.7 0 Td 48.611111 Tz (Additionally, ) Tj 4.005556 0 Td 55.092593 Tz (each ) Tj 1.555556 0 Td 57.253086 Tz (sample ) Tj 2.275 0 Td 61.574074 Tz (was ) Tj 51.428571 0 0 51.428571 0 379.896471 Tm 25.316667 0 Td 50.771605 Tz (Subjected ) Tj 2.858333 0 Td 46.990741 Tz (to ) Tj 0.719444 0 Td 59.722222 Tz (genomic ) Tj 2.683333 0 Td 85.339506 Tz (DNA ) Tj 1.711111 0 Td 51.388889 Tz (contamination, ) Tj 4.491667 0 Td 49.074074 Tz (reverse ) Tj 2.177778 0 Td 51.851852 Tz (transcrip ) Tj 51.428571 0 0 51.428571 0 336.687805 Tm 25.277778 0 Td 44.074074 Tz (tion, ) Tj 1.497222 0 Td 60.493827 Tz (and ) Tj 1.263889 0 Td 56.064815 Tz (polymerase ) Tj 3.558333 0 Td 51.851852 Tz (chain ) Tj 1.75 0 Td 48.611111 Tz (reaction ) Tj 2.566667 0 Td 48.148148 Tz (control ) Tj 2.216667 0 Td 48.611111 Tz (reactions ) Tj 2.819444 0 Td 59.953704 Tz (on ) Tj 0.913889 0 Td 50.771605 Tz (the ) Tj 51.428571 0 0 51.428571 0 296.539823 Tm 25.297222 0 Td 57.79321 Tz (custom ) Tj 2.333333 0 Td 45.37037 Tz (built ) Tj 1.575 0 Td 44.444444 Tz (plates. ) Tj 2.158333 0 Td 58.333333 Tz (Ct ) Tj 0.972222 0 Td 50.231481 Tz (values ) Tj 2.080556 0 Td 48.611111 Tz (greater ) Tj 2.294444 0 Td 50.231481 Tz (than ) Tj 1.497222 0 Td 43.897306 Tz (thirty-five ) Tj 3.15 0 Td 59.143519 Tz (were ) Tj 40 0 0 40 0 259 Tm 32.5 0 Td 67.5 Tz (taken ) Tj 2.25 0 Td 66.666667 Tz (as ) Tj 1.025 0 Td 75 Tz (no ) Tj 1.575 0 Td 55.555556 Tz (call. ) Tj 51.428571 0 0 51.428571 0 211.285024 Tm 26.016667 0 Td 47.115385 Tz (Proliferation ) Tj 3.811111 0 Td 51.041667 Tz (data ) Tj 1.341667 0 Td 59.143519 Tz (were ) Tj 1.536111 0 Td 53.877315 Tz (analyzed ) Tj 2.722222 0 Td 52.662037 Tz (with ) Tj 1.4 0 Td 70.601852 Tz (JMPSTATISTICAL ) Tj 51.428571 0 0 51.428571 0 168.80305 Tm 25.297222 0 Td 75.617284 Tz (DISCOVERY ) Tj 4.491667 0 Td 79.803241 Tz (SOFTWARE ) Tj 4.238889 0 Td 65.625 Tz (\(SAS ) Tj 2.022222 0 Td 42.12963 Tz (Institute; ) Tj 2.955556 0 Td 51.203704 Tz (Cary, ) Tj 1.983333 0 Td 48.611111 Tz (N.C.\). ) Tj ET Q endstream endobj 462 0 obj << /Length 1 >> stream q endstream endobj 463 0 obj << /Length 51139 >> stream Q q 0.24 0 0 0.24 0 0 cm BT /F1 1 Tf 3 Tr 62.857143 0 0 62.857143 0 3023 Tm 17.325 0 Td 83.522727 Tz (US ) Tj 1.240909 0 Td 58.333333 Tz (8,404,256 ) Tj 3.388636 0 Td 74.242424 Tz (B2 ) Tj 52.857143 0 0 52.857143 0 2968 Tm 14.245946 0 Td 72.522523 Tz (13 ) Tj 51.428571 0 0 51.428571 0 2916.510303 Tm 6.377778 0 Td 85.339506 Tz (DNA ) Tj 1.691667 0 Td 50.231481 Tz (values ) Tj 1.983333 0 Td 58.333333 Tz (were ) Tj 1.555556 0 Td 50.411523 Tz (subjected ) Tj 2.877778 0 Td 43.75 Tz (to ) Tj 0.661111 0 Td 51.851852 Tz (a ) Tj 0.486111 0 Td 42.12963 Tz (full ) Tj 1.147222 0 Td 44.650206 Tz (factorial ) Tj 2.586111 0 Td 59.259259 Tz (two-way ) Tj 2.625 0 Td 60.925926 Tz (analy ) Tj 51.428571 0 0 51.428571 0 2872 Tm 6.377778 0 Td 41.049383 Tz (sis ) Tj 0.894444 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 51.041667 Tz (variance ) Tj 2.586111 0 Td 54.6875 Tz (\(C=0.05\) ) Tj 2.8 0 Td 45.37037 Tz (to ) Tj 0.738889 0 Td 48.070988 Tz (detect ) Tj 1.847222 0 Td 45.833333 Tz (effects ) Tj 2.1 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 48.611111 Tz (culture ) Tj 2.197222 0 Td 45.694444 Tz (substrate, ) Tj 51.428571 0 0 51.428571 0 2831.995227 Tm 6.338889 0 Td 55.092593 Tz (time ) Tj 1.458333 0 Td 45.37037 Tz (in ) Tj 0.719444 0 Td 45.37037 Tz (culture, ) Tj 2.333333 0 Td 58.333333 Tz (and ) Tj 1.166667 0 Td 46.018519 Tz (their ) Tj 1.516667 0 Td 45.100309 Tz (interaction. ) Tj 3.441667 0 Td 52.256944 Tz (Post-hoc ) Tj 2.644444 0 Td 51.446759 Tz (analyses ) Tj 2.625 0 Td 53.148148 Tz (using ) Tj 51.428571 0 0 51.428571 0 2791.871605 Tm 6.358333 0 Td 55.555556 Tz (Tukey's ) Tj 2.625 0 Td 77.777778 Tz (HSD ) Tj 1.633333 0 Td 60.493827 Tz (method ) Tj 2.411111 0 Td 54.282407 Tz (\(C0.05\) ) Tj 2.838889 0 Td 58.333333 Tz (were ) Tj 1.633333 0 Td 56.17284 Tz (performed ) Tj 3.266667 0 Td 45.37037 Tz (to ) Tj 0.816667 0 Td 46.990741 Tz (identify ) Tj 51.428571 0 0 51.428571 0 2748.924731 Tm 6.358333 0 Td 48.611111 Tz (differences ) Tj 3.616667 0 Td 57.87037 Tz (between ) Tj 2.897222 0 Td 46.990741 Tz (factor ) Tj 2.1 0 Td 43.055556 Tz (levels. ) Tj 2.275 0 Td 55.092593 Tz (Fold ) Tj 1.711111 0 Td 57.79321 Tz (change ) Tj 2.527778 0 Td 43.75 Tz (in ) Tj 0.952778 0 Td 58.333333 Tz (gene ) Tj 51.428571 0 0 51.428571 0 2707.462324 Tm 6.358333 0 Td 51.527778 Tz (expression ) Tj 3.383333 0 Td 61.574074 Tz (was ) Tj 1.419444 0 Td 48.935185 Tz (calculated ) Tj 3.208333 0 Td 53.148148 Tz (using ) Tj 1.847222 0 Td 50.771605 Tz (the ) Tj 1.205556 0 Td 46.018519 Tz (delta ) Tj 1.672222 0 Td 47.314815 Tz (delta ) Tj 1.672222 0 Td 39.969136 Tz (Clt ) Tj 0.991667 0 Td 57.253086 Tz (\(AACt\) ) Tj 51.428571 0 0 51.428571 0 2666.10687 Tm 6.358333 0 Td 56.018519 Tz (method, ) Tj 2.683333 0 Td 58.981481 Tz (where ) Tj 2.158333 0 Td 45.833333 Tz (B-actin ) Tj 2.255556 0 Td 51.851852 Tz (served ) Tj 2.158333 0 Td 50.231481 Tz (as ) Tj 0.875 0 Td 50.771605 Tz (the ) Tj 1.205556 0 Td 55.902778 Tz (housekeeping ) Tj 4.297222 0 Td 50.771605 Tz (\(refer ) Tj 51.428571 0 0 51.428571 0 2622.702101 Tm 6.358333 0 Td 53.148148 Tz (ence\) ) Tj 1.769444 0 Td 57.523148 Tz (gene ) Tj 1.575 0 Td 48.611111 Tz (for ) Tj 1.05 0 Td 51.353276 Tz (normalization ) Tj 4.2 0 Td 59.41358 Tz (and ) Tj 1.263889 0 Td 53.472222 Tz (time ) Tj 1.477778 0 Td 51.041667 Tz (Zero ) Tj 1.438889 0 Td 75.347222 Tz (MSCs ) Tj 2.022222 0 Td 52.391975 Tz (served ) Tj 2.061111 0 Td 50.231481 Tz (as ) Tj 51.428571 0 0 51.428571 0 2583.435669 Tm 6.338889 0 Td 50.771605 Tz (the ) Tj 1.147222 0 Td 49.074074 Tz (control ) Tj 2.294444 0 Td 52.391975 Tz (group. ) Tj 2.177778 0 Td 50.555556 Tz (Delta ) Tj 1.769444 0 Td 58.333333 Tz (Ct ) Tj 0.933333 0 Td 54.444444 Tz (\(ACt\) ) Tj 1.886111 0 Td 50.231481 Tz (values ) Tj 2.061111 0 Td 59.143519 Tz (were ) Tj 1.652778 0 Td 51.131687 Tz (subjected ) Tj 2.994444 0 Td 46.990741 Tz (to ) Tj 51.428571 0 0 51.428571 0 2540.895238 Tm 6.377778 0 Td 41.245791 Tz (statistical ) Tj 2.838889 0 Td 49.016204 Tz (analysis ) Tj 2.488889 0 Td 52.662037 Tz (with ) Tj 1.419444 0 Td 74.537037 Tz (SASR ) Tj 2.022222 0 Td 54.282407 Tz (Software ) Tj 2.722222 0 Td 64.814815 Tz (\(SAS ) Tj 1.730556 0 Td 42.12963 Tz (Institute; ) Tj 2.683333 0 Td 50.555556 Tz (Cary, ) Tj 51.428571 0 0 51.428571 0 2498.92629 Tm 6.358333 0 Td 53.796296 Tz (N.C.\) ) Tj 1.827778 0 Td 53.148148 Tz (using ) Tj 1.827778 0 Td 45.910494 Tz (linear ) Tj 1.886111 0 Td 50.356125 Tz (mixed-effects ) Tj 4.180556 0 Td 57.79321 Tz (models ) Tj 2.313889 0 Td 52.777778 Tz (through ) Tj 2.45 0 Td 49.691358 Tz (the ) Tj 1.127778 0 Td 76.967593 Tz (PROC ) Tj 51.428571 0 0 51.428571 0 2457.430274 Tm 6.358333 0 Td 79.722222 Tz (MIXED ) Tj 2.566667 0 Td 54.012346 Tz (procedure ) Tj 3.091667 0 Td 59.41358 Tz (and ) Tj 1.263889 0 Td 68.518519 Tz (Minimum ) Tj 3.072222 0 Td 52.662037 Tz (Variance ) Tj 2.741667 0 Td 52.572016 Tz (Quadratic ) Tj 3.033333 0 Td 70.486111 Tz (Unbi ) Tj 51.428571 0 0 51.428571 0 2418.240741 Tm 6.358333 0 Td 54.282407 Tz (ased ) Tj 1.477778 0 Td 51.203704 Tz (Estimation ) Tj 3.208333 0 Td 73.321759 Tz (\(MIVQUEO ) Tj 3.636111 0 Td 46.990741 Tz (option\). ) Tj 2.391667 0 Td 57.79321 Tz (Weight ) Tj 2.197222 0 Td 50.771605 Tz (percentalginate ) Tj 51.428571 0 0 51.428571 0 2374.284672 Tm 6.358333 0 Td 58.333333 Tz (and ) Tj 1.186111 0 Td 53.472222 Tz (presence ) Tj 2.702778 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 72.916667 Tz (hCPR ) Tj 1.925 0 Td 58.333333 Tz (were ) Tj 1.555556 0 Td 51.203704 Tz (taken ) Tj 1.672222 0 Td 51.851852 Tz (as ) Tj 0.777778 0 Td 48.611111 Tz (fixed ) Tj 1.575 0 Td 46.296296 Tz (effects ) Tj 2.080556 0 Td 59.41358 Tz (and ) Tj 1.225 0 Td 54.282407 Tz (time ) Tj 1.438889 0 Td 50.231481 Tz (in ) Tj 40 0 0 40 0 2339 Tm 8.175 0 Td 61.904762 Tz (culture ) Tj 2.85 0 Td 77.777778 Tz (was ) Tj 1.65 0 Td 66.666667 Tz (taken ) Tj 2.225 0 Td 64.583333 Tz (as ) Tj 1 0 Td 75 Tz (a ) Tj 0.675 0 Td 78.472222 Tz (random ) Tj 3.05 0 Td 55.952381 Tz (effect. ) Tj 2.625 0 Td 60.984848 Tz (Interaction ) Tj 4.25 0 Td 69.166667 Tz (terms ) Tj 2.35 0 Td 75 Tz (were ) Tj 51.428571 0 0 51.428571 0 2292.352941 Tm 6.358333 0 Td 52.932099 Tz (not ) Tj 1.127778 0 Td 49.789562 Tz (considered. ) Tj 3.5 0 Td 48.021886 Tz (Significant ) Tj 3.344444 0 Td 47.962963 Tz (fixed ) Tj 1.613889 0 Td 45.37037 Tz (effects ) Tj 2.1 0 Td 59.143519 Tz (were ) Tj 1.594444 0 Td 50.636574 Tz (detected ) Tj 2.605556 0 Td 53.148148 Tz (using ) Tj 51.428571 0 0 51.428571 0 2249.804296 Tm 6.358333 0 Td 59.953704 Tz (Type ) Tj 1.613889 0 Td 37.808642 Tz (III ) Tj 0.836111 0 Td 67.24537 Tz (Sums ) Tj 1.730556 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 53.240741 Tz (Squares ) Tj 2.430556 0 Td 45.37037 Tz (F-tests ) Tj 2.061111 0 Td 54.6875 Tz (\(C=0.05\) ) Tj 2.780556 0 Td 52.662037 Tz (with ) Tj 1.419444 0 Td 49.109687 Tz (Satterthwaite ) Tj 51.428571 0 0 51.428571 0 2209.245283 Tm 6.358333 0 Td 53.703704 Tz (approximations ) Tj 4.686111 0 Td 48.611111 Tz (for ) Tj 0.991667 0 Td 56.271044 Tz (denominator ) Tj 3.85 0 Td 51.851852 Tz (degrees ) Tj 2.352778 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 56.944444 Tz (freedom ) Tj 2.527778 0 Td 60.493827 Tz (and ) Tj 1.244444 0 Td 58.333333 Tz (fur ) Tj 51.428571 0 0 51.428571 0 2168.994865 Tm 6.338889 0 Td 49.421296 Tz (ther ) Tj 1.458333 0 Td 50.411523 Tz (subjected ) Tj 3.033333 0 Td 43.75 Tz (to ) Tj 0.797222 0 Td 55.555556 Tz (Tukey's ) Tj 2.663889 0 Td 78.858025 Tz (HSD ) Tj 1.672222 0 Td 52.256944 Tz (post-hoc ) Tj 2.819444 0 Td 42.777778 Tz (tests ) Tj 1.594444 0 Td 54.282407 Tz (\(C=0.05\) ) Tj 2.877778 0 Td 46.990741 Tz (to ) Tj 51.428571 0 0 51.428571 0 2125.710588 Tm 6.358333 0 Td 48.611111 Tz (detect ) Tj 1.866667 0 Td 48.611111 Tz (differences ) Tj 3.325 0 Td 66.759259 Tz (among ) Tj 2.138889 0 Td 48.611111 Tz (the ) Tj 0.991667 0 Td 48.070988 Tz (factor ) Tj 1.847222 0 Td 43.055556 Tz (levels. ) Tj 1.963889 0 Td 49.96142 Tz (Significance ) Tj 3.713889 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 2082.907331 Tm 6.358333 0 Td 61.033951 Tz (random ) Tj 2.741667 0 Td 48.206019 Tz (variable ) Tj 2.8 0 Td 61.574074 Tz (was ) Tj 1.613889 0 Td 50.636574 Tz (detected ) Tj 2.897222 0 Td 53.148148 Tz (using ) Tj 2.061111 0 Td 50.771605 Tz (the ) Tj 1.380556 0 Td 63.194444 Tz (Wald ) Tj 1.983333 0 Td 47.222222 Tz (Z-tests ) Tj 51.428571 0 0 51.428571 0 2042.705314 Tm 6.377778 0 Td 38.888889 Tz (\(C. ) Tj 1.088889 0 Td 50.555556 Tz (0.05\) ) Tj 1.672222 0 Td 58.333333 Tz (on ) Tj 0.816667 0 Td 50.771605 Tz (the ) Tj 1.05 0 Td 51.041667 Tz (variance ) Tj 2.566667 0 Td 54.372428 Tz (parameter ) Tj 3.013889 0 Td 50.411523 Tz (estimates ) Tj 2.858333 0 Td 61.574074 Tz (on ) Tj 0.836111 0 Td 50.771605 Tz (the ) Tj 1.030556 0 Td 52.256944 Tz (diagonal ) Tj 51.428571 0 0 51.428571 0 2001.470886 Tm 6.358333 0 Td 58.333333 Tz (of ) Tj 0.991667 0 Td 48.611111 Tz (the ) Tj 1.205556 0 Td 52.5 Tz (covariance ) Tj 3.538889 0 Td 50.231481 Tz (matrices ) Tj 2.780556 0 Td 72.511574 Tz (\(COVTEST ) Tj 3.85 0 Td 47.395833 Tz (option\); ) Tj 2.625 0 Td 51.446759 Tz (p-values ) Tj 51.428571 0 0 51.428571 0 1959.188034 Tm 6.338889 0 Td 59.953704 Tz (were ) Tj 1.594444 0 Td 51.203704 Tz (taken ) Tj 1.672222 0 Td 50.231481 Tz (as ) Tj 0.738889 0 Td 49.421296 Tz (half ) Tj 1.263889 0 Td 55.092593 Tz (of ) Tj 0.758333 0 Td 45.37037 Tz (that ) Tj 1.225 0 Td 50.231481 Tz (reported ) Tj 2.547222 0 Td 57.253086 Tz (due ) Tj 1.186111 0 Td 45.37037 Tz (to ) Tj 0.680556 0 Td 46.296296 Tz (testing ) Tj 2.061111 0 Td 45.37037 Tz (at ) Tj 0.7 0 Td 48.611111 Tz (the ) Tj 0.991667 0 Td 68.703704 Tz (bound ) Tj 51.428571 0 0 51.428571 0 1916.957611 Tm 6.358333 0 Td 50.771605 Tz (ary ) Tj 1.127778 0 Td 50.411523 Tz (condition ) Tj 2.936111 0 Td 49.691358 Tz (\(variance ) Tj 2.858333 0 Td 51.851852 Tz (equal ) Tj 1.730556 0 Td 46.990741 Tz (to ) Tj 0.758333 0 Td 45.910494 Tz (Zero\). ) Tj 51.428571 0 0 51.428571 0 1877.590909 Tm 7.097222 0 Td 75.617284 Tz (Now ) Tj 1.769444 0 Td 46.8107 Tz (referring ) Tj 2.877778 0 Td 45.37037 Tz (to ) Tj 0.952778 0 Td 52.662037 Tz (FIG. ) Tj 1.652778 0 Td 43.75 Tz (2. ) Tj 0.913889 0 Td 54.62963 Tz (reduced ) Tj 2.644444 0 Td 46.367521 Tz (proliferation ) Tj 4.005556 0 Td 44.560185 Tz (rate ) Tj 1.419444 0 Td 40.509259 Tz (is ) Tj 51.428571 0 0 51.428571 0 1836.929193 Tm 6.358333 0 Td 52.662037 Tz (apparent ) Tj 2.722222 0 Td 43.75 Tz (in ) Tj 0.719444 0 Td 50.771605 Tz (the ) Tj 1.088889 0 Td 53.06713 Tz (presence ) Tj 2.722222 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 49.421296 Tz (gels ) Tj 1.380556 0 Td 45.37037 Tz (in ) Tj 0.758333 0 Td 56.388889 Tz (comparison ) Tj 3.577778 0 Td 46.990741 Tz (to ) Tj 0.797222 0 Td 76.967593 Tz (mono ) Tj 51.428571 0 0 51.428571 0 1794.252196 Tm 6.358333 0 Td 49.259259 Tz (layer ) Tj 1.691667 0 Td 54.372428 Tz (expansion ) Tj 3.169444 0 Td 61.574074 Tz (on ) Tj 1.030556 0 Td 45.910494 Tz (tissue ) Tj 1.886111 0 Td 48.148148 Tz (culture ) Tj 2.255556 0 Td 44.907407 Tz (plastic ) Tj 2.177778 0 Td 54.012346 Tz (\(TCP\). ) Tj 2.177778 0 Td 87.5 Tz (A ) Tj 0.797222 0 Td 55.092593 Tz (reduced ) Tj 51.428571 0 0 51.428571 0 1752.046707 Tm 6.358333 0 Td 46.367521 Tz (proliferation ) Tj 3.791667 0 Td 44.560185 Tz (rate ) Tj 1.166667 0 Td 51.851852 Tz (as ) Tj 0.758333 0 Td 53.472222 Tz (seen ) Tj 1.438889 0 Td 43.75 Tz (in ) Tj 0.641667 0 Td 51.851852 Tz (the ) Tj 1.030556 0 Td 53.472222 Tz (presence ) Tj 2.683333 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 49.691358 Tz (the ) Tj 1.011111 0 Td 49.691358 Tz (biomaterials ) Tj 3.713889 0 Td 40.509259 Tz (is ) Tj 51.428571 0 0 51.428571 0 1710 Tm 6.358333 0 Td 48.611111 Tz (consistent ) Tj 3.188889 0 Td 52.662037 Tz (with ) Tj 1.575 0 Td 59.806397 Tz (mesenchymal ) Tj 4.219444 0 Td 56.712963 Tz (stem ) Tj 1.613889 0 Td 44.560185 Tz (cell ) Tj 1.341667 0 Td 45.802469 Tz (differentiation ) Tj 4.413889 0 Td 46.180556 Tz (into ) Tj 51.428571 0 0 51.428571 0 1672.594465 Tm 6.358333 0 Td 53.364198 Tz (nonhypertrophic ) Tj 5.075 0 Td 51.353276 Tz (chondrocytes. ) Tj 4.297222 0 Td 56.712963 Tz (Data ) Tj 1.594444 0 Td 61.574074 Tz (show ) Tj 1.75 0 Td 55.092593 Tz (groups ) Tj 2.216667 0 Td 52.662037 Tz (averages ) Tj 52.857143 0 0 52.857143 0 1623 Tm 6.205405 0 Td 52.342342 Tz (\(n-3\) ) Tj 1.740541 0 Td 56.756757 Tz (and ) Tj 1.210811 0 Td 53.603604 Tz (-/- ) Tj 1.154054 0 Td 56.756757 Tz (one ) Tj 1.210811 0 Td 49.662162 Tz (standard ) Tj 2.572973 0 Td 46.036036 Tz (deviation. ) Tj 57.142857 0 0 57.142857 0 1585.34375 Tm 6.405 0 Td 52.5 Tz (5x10" ) Tj 1.82 0 Td 50.069444 Tz (second ) Tj 2.03 0 Td 48.333333 Tz (passage ) Tj 2.275 0 Td 39.861111 Tz (rat ) Tj 0.91 0 Td 54.356061 Tz (mesenchymal ) Tj 3.8325 0 Td 51.770833 Tz (stem ) Tj 1.4525 0 Td 40.25 Tz (cells ) Tj 1.4525 0 Td 53.229167 Tz (were ) Tj 51.428571 0 0 51.428571 0 1543.237113 Tm 6.377778 0 Td 54.552469 Tz (seeded ) Tj 2.216667 0 Td 52.662037 Tz (onto ) Tj 1.594444 0 Td 32.407407 Tz (1 ) Tj 0.544444 0 Td 61.574074 Tz (ml ) Tj 1.011111 0 Td 55.092593 Tz (samples ) Tj 2.586111 0 Td 56.712963 Tz (of ) Tj 0.816667 0 Td 50.771605 Tz (the ) Tj 1.127778 0 Td 52.662037 Tz (experimental ) Tj 4.083333 0 Td 48.251029 Tz (materials ) Tj 2.877778 0 Td 51.851852 Tz (or ) Tj 51.428571 0 0 51.428571 0 1499.954933 Tm 6.358333 0 Td 53.472222 Tz (onto ) Tj 1.516667 0 Td 45.910494 Tz (tissue ) Tj 1.866667 0 Td 48.148148 Tz (culture ) Tj 2.216667 0 Td 45.37037 Tz (plastic ) Tj 2.119444 0 Td 55.092593 Tz (\(TCP\). ) Tj 2.197222 0 Td 51.446759 Tz (Cultures ) Tj 2.702778 0 Td 59.143519 Tz (were ) Tj 1.652778 0 Td 53.796296 Tz (maintained ) Tj 51.428571 0 0 51.428571 0 1459.494845 Tm 6.358333 0 Td 49.691358 Tz (for ) Tj 1.127778 0 Td 48.611111 Tz (the ) Tj 1.166667 0 Td 49.537037 Tz (various ) Tj 2.352778 0 Td 53.472222 Tz (time ) Tj 1.516667 0 Td 49.691358 Tz (points ) Tj 2.080556 0 Td 56.17284 Tz (shown. ) Tj 2.313889 0 Td 85.339506 Tz (DNA ) Tj 1.808333 0 Td 60.493827 Tz (was ) Tj 1.361111 0 Td 48.611111 Tz (quantified ) Tj 3.169444 0 Td 50.231481 Tz (in ) Tj 51.428571 0 0 51.428571 0 1418.237392 Tm 6.358333 0 Td 46.759259 Tz (lysates ) Tj 2.216667 0 Td 52.5 Tz (using ) Tj 1.788889 0 Td 51.851852 Tz (a ) Tj 0.563889 0 Td 56.712963 Tz (commercial ) Tj 3.636111 0 Td 43.209877 Tz (kit ) Tj 1.011111 0 Td 57.685185 Tz (which ) Tj 1.963889 0 Td 59.548611 Tz (employed ) Tj 3.111111 0 Td 48.316498 Tz (fluorescent ) Tj 51.428571 0 0 51.428571 0 1376.964143 Tm 6.358333 0 Td 59.41358 Tz (dye ) Tj 1.361111 0 Td 49.421296 Tz (binding. ) Tj 2.702778 0 Td 49.691358 Tz (All ) Tj 1.205556 0 Td 55.092593 Tz (groups ) Tj 2.275 0 Td 50.411523 Tz (exhibited ) Tj 3.033333 0 Td 55.092593 Tz (an ) Tj 1.011111 0 Td 39.351852 Tz (initial ) Tj 1.983333 0 Td 52.5 Tz (Small ) Tj 1.886111 0 Td 46.990741 Tz (loss ) Tj 1.458333 0 Td 48.611111 Tz (in ) Tj 51.428571 0 0 51.428571 0 1337.762712 Tm 6.358333 0 Td 44.650206 Tz (viability ) Tj 2.605556 0 Td 58.333333 Tz (by ) Tj 0.913889 0 Td 56.17284 Tz (day ) Tj 1.186111 0 Td 49.259259 Tz (three ) Tj 1.652778 0 Td 54.12037 Tz (\(p=0.0002\) ) Tj 3.383333 0 Td 52.932099 Tz (but ) Tj 1.108333 0 Td 56.388889 Tz (group ) Tj 1.866667 0 Td 52.256944 Tz (averages ) Tj 2.683333 0 Td 57.253086 Tz (contin ) Tj 51.428571 0 0 51.428571 0 1292.877653 Tm 6.338889 0 Td 59.41358 Tz (ued ) Tj 1.302778 0 Td 45.37037 Tz (to ) Tj 0.777778 0 Td 42.939815 Tz (rise ) Tj 1.205556 0 Td 44.191919 Tz (thereafter. ) Tj 3.130556 0 Td 62.654321 Tz (The ) Tj 1.302778 0 Td 46.866097 Tz (proliferation ) Tj 3.908333 0 Td 44.074074 Tz (rates ) Tj 1.536111 0 Td 59.953704 Tz (on ) Tj 0.952778 0 Td 48.611111 Tz (the ) Tj 1.05 0 Td 64.274691 Tz (concen ) Tj 51.428571 0 0 51.428571 0 1256.160434 Tm 6.338889 0 Td 46.990741 Tz (trated ) Tj 2.002778 0 Td 45.37037 Tz (platelet ) Tj 2.527778 0 Td 48.611111 Tz (releasate-containing ) Tj 6.163889 0 Td 50.028935 Tz (alginate/calcium ) Tj 5.172222 0 Td 67.24537 Tz (phos ) Tj 51.428571 0 0 51.428571 0 1212.970964 Tm 6.358333 0 Td 53.148148 Tz (phate ) Tj 1.691667 0 Td 55.452675 Tz (composite ) Tj 3.111111 0 Td 48.971193 Tz (materials ) Tj 2.761111 0 Td 59.143519 Tz (were ) Tj 1.536111 0 Td 47.530864 Tz (similarly ) Tj 2.722222 0 Td 54.166667 Tz (reduced ) Tj 2.391667 0 Td 54.12037 Tz (\(p=0.1082\) ) Tj 51.428571 0 0 51.428571 0 1170.503113 Tm 6.358333 0 Td 45.37037 Tz (in ) Tj 0.816667 0 Td 56.064815 Tz (comparison ) Tj 3.597222 0 Td 46.990741 Tz (to ) Tj 0.797222 0 Td 50.771605 Tz (the ) Tj 1.127778 0 Td 47.800926 Tz (positive ) Tj 2.508333 0 Td 48.611111 Tz (control ) Tj 2.313889 0 Td 57.973251 Tz (monolayer ) Tj 3.344444 0 Td 47.685185 Tz (culture ) Tj 2.236111 0 Td 63.194444 Tz (on ) Tj 51.428571 0 0 51.428571 0 1127.590244 Tm 6.338889 0 Td 50.771605 Tz (the ) Tj 1.147222 0 Td 45.910494 Tz (tissue ) Tj 1.847222 0 Td 48.611111 Tz (culture ) Tj 2.255556 0 Td 44.907407 Tz (plastic ) Tj 2.138889 0 Td 51.557239 Tz (\(p<0.0001\). ) Tj 3.616667 0 Td 54.282407 Tz (This ) Tj 1.536111 0 Td 53.292181 Tz (continued ) Tj 3.072222 0 Td 51.851852 Tz (prolif ) Tj 51.428571 0 0 51.428571 0 1086.778313 Tm 6.358333 0 Td 47.685185 Tz (eration ) Tj 2.216667 0 Td 38.888889 Tz (is ) Tj 0.661111 0 Td 48.611111 Tz (consistent ) Tj 3.091667 0 Td 51.851852 Tz (with ) Tj 1.458333 0 Td 58.333333 Tz (gene ) Tj 1.555556 0 Td 51.527778 Tz (expression ) Tj 3.286111 0 Td 44.907407 Tz (results ) Tj 2.080556 0 Td 52.175926 Tz (suggesting ) Tj 51.428571 0 0 51.428571 0 1048.196262 Tm 6.358333 0 Td 44.074074 Tz (cells ) Tj 1.497222 0 Td 51.851852 Tz (displayed ) Tj 2.936111 0 Td 58.333333 Tz (a ) Tj 0.486111 0 Td 46.118234 Tz (proliferative ) Tj 3.791667 0 Td 52.716049 Tz (nonhypertrophic ) Tj 4.919444 0 Td 58.038721 Tz (chondrocyte ) Tj 51.428571 0 0 51.428571 0 1005.972851 Tm 6.358333 0 Td 45.37037 Tz (like ) Tj 1.244444 0 Td 53.472222 Tz (phenotype. ) Tj 51.428571 0 0 51.428571 0 961.206266 Tm 7.097222 0 Td 62.654321 Tz (The ) Tj 1.438889 0 Td 84.259259 Tz (MSC ) Tj 1.808333 0 Td 56.712963 Tz (growth ) Tj 2.352778 0 Td 58.333333 Tz (example ) Tj 2.741667 0 Td 54.552469 Tz (demonstrated ) Tj 4.238889 0 Td 49.016204 Tz (material ) Tj 2.663889 0 Td 61.574074 Tz (cyto ) Tj 51.428571 0 0 51.428571 0 922.733493 Tm 6.358333 0 Td 49.608262 Tz (compatibility ) Tj 4.083333 0 Td 53.703704 Tz (through ) Tj 2.488889 0 Td 53.796296 Tz (maintained ) Tj 3.461111 0 Td 43.930041 Tz (viability ) Tj 2.566667 0 Td 48.611111 Tz (early ) Tj 1.691667 0 Td 45.37037 Tz (after ) Tj 1.516667 0 Td 68.865741 Tz (expo ) Tj 51.428571 0 0 51.428571 0 879.949569 Tm 6.377778 0 Td 49.421296 Tz (sure ) Tj 1.438889 0 Td 43.75 Tz (to ) Tj 0.758333 0 Td 50.771605 Tz (the ) Tj 1.147222 0 Td 48.611111 Tz (material ) Tj 2.566667 0 Td 59.41358 Tz (and ) Tj 1.283333 0 Td 49.826389 Tz (biocompatibility ) Tj 5.036111 0 Td 53.240741 Tz (through ) Tj 2.45 0 Td 51.131687 Tz (prolifera ) Tj 51.428571 0 0 51.428571 0 835.438662 Tm 6.338889 0 Td 45.37037 Tz (tive ) Tj 1.283333 0 Td 53.472222 Tz (behavior ) Tj 2.741667 0 Td 43.425926 Tz (later ) Tj 1.477778 0 Td 45.37037 Tz (in ) Tj 0.758333 0 Td 49.691358 Tz (the ) Tj 1.088889 0 Td 58.796296 Tz (example ) Tj 2.663889 0 Td 51.851852 Tz (\(FIG. ) Tj 1.788889 0 Td 42.12963 Tz (2\). ) Tj 0.952778 0 Td 61.033951 Tz (Growth ) Tj 2.411111 0 Td 48.206019 Tz (patterns ) Tj 51.428571 0 0 51.428571 0 794.969267 Tm 6.358333 0 Td 49.907407 Tz (associated ) Tj 3.188889 0 Td 52.662037 Tz (with ) Tj 1.438889 0 Td 54.282407 Tz (both ) Tj 1.477778 0 Td 52.662037 Tz (experimental ) Tj 3.986111 0 Td 51.58179 Tz (formulations ) Tj 3.869444 0 Td 59.41358 Tz (and ) Tj 1.205556 0 Td 48.206019 Tz (positive ) Tj 51.428571 0 0 51.428571 0 752.565111 Tm 6.358333 0 Td 49.074074 Tz (control ) Tj 2.294444 0 Td 75.617284 Tz (TCP ) Tj 1.575 0 Td 54.552469 Tz (demonstrated ) Tj 4.180556 0 Td 52.662037 Tz (very ) Tj 1.536111 0 Td 48.148148 Tz (similar ) Tj 2.216667 0 Td 46.450617 Tz (patterns, ) Tj 2.780556 0 Td 54.282407 Tz (drop ) Tj 1.536111 0 Td 46.018519 Tz (after ) Tj 51.428571 0 0 51.428571 0 714.005848 Tm 6.377778 0 Td 52.777778 Tz (seeding ) Tj 2.294444 0 Td 54.282407 Tz (followed ) Tj 2.683333 0 Td 61.574074 Tz (by ) Tj 0.894444 0 Td 43.055556 Tz (rising, ) Tj 1.925 0 Td 53.472222 Tz (with ) Tj 1.458333 0 Td 48.611111 Tz (material ) Tj 2.45 0 Td 52.211934 Tz (presences ) Tj 2.936111 0 Td 52.211934 Tz (hindering ) Tj 51.428571 0 0 51.428571 0 670.197957 Tm 6.338889 0 Td 50.771605 Tz (the ) Tj 1.166667 0 Td 54.372428 Tz (expansion ) Tj 3.227778 0 Td 42.12963 Tz (rates. ) Tj 1.808333 0 Td 47.727273 Tz (Significant ) Tj 3.402778 0 Td 45.37037 Tz (effects ) Tj 2.177778 0 Td 59.953704 Tz (of ) Tj 0.855556 0 Td 54.282407 Tz (both ) Tj 1.575 0 Td 49.691358 Tz (the ) Tj 1.166667 0 Td 53.472222 Tz (time ) Tj 1.536111 0 Td 50.231481 Tz (in ) Tj 40 0 0 40 0 634 Tm 8.175 0 Td 61.904762 Tz (culture ) Tj 2.875 0 Td 76.388889 Tz (and ) Tj 1.675 0 Td 61.309524 Tz (culture ) Tj 2.85 0 Td 61.574074 Tz (Substrate ) Tj 3.625 0 Td 76.041667 Tz (main ) Tj 2.125 0 Td 60.119048 Tz (factors ) Tj 2.825 0 Td 75 Tz (and ) Tj 1.625 0 Td 58.333333 Tz (their ) Tj 2.025 0 Td 67.261905 Tz (interac ) Tj 51.428571 0 0 51.428571 0 585.811094 Tm 6.338889 0 Td 47.800926 Tz (tion ) Tj 1.711111 0 Td 54.372428 Tz (\(p<0.0001 ) Tj 3.577778 0 Td 47.530864 Tz (for ) Tj 1.380556 0 Td 40.509259 Tz (all\) ) Tj 1.516667 0 Td 59.143519 Tz (were ) Tj 1.983333 0 Td 50.231481 Tz (detected ) Tj 2.955556 0 Td 45.37037 Tz (in ) Tj 1.108333 0 Td 50.771605 Tz (the ) Tj 1.477778 0 Td 58.981481 Tz (model ) Tj 51.428571 0 0 51.428571 0 546.229885 Tm 6.377778 0 Td 51.557239 Tz (\(p<0.0001\). ) Tj 3.830556 0 Td 39.861111 Tz (Initially, ) Tj 2.741667 0 Td 53.703704 Tz (average ) Tj 2.644444 0 Td 84.259259 Tz (DNA ) Tj 1.905556 0 Td 50.231481 Tz (values ) Tj 2.158333 0 Td 51.446759 Tz (declined ) Tj 2.819444 0 Td 46.018519 Tz (after ) Tj 51.428571 0 0 51.428571 0 505.698565 Tm 6.377778 0 Td 53.240741 Tz (seeding ) Tj 2.333333 0 Td 64.00463 Tz (when ) Tj 1.711111 0 Td 59.143519 Tz (compared ) Tj 2.994444 0 Td 45.37037 Tz (to ) Tj 0.680556 0 Td 58.333333 Tz (day ) Tj 1.205556 0 Td 47.962963 Tz (three ) Tj 1.575 0 Td 51.851852 Tz (\(p=0.0002\), ) Tj 3.538889 0 Td 50.771605 Tz (but ) Tj 1.05 0 Td 46.018519 Tz (after ) Tj 1.477778 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 463.506739 Tm 6.358333 0 Td 57.253086 Tz (day ) Tj 1.477778 0 Td 49.259259 Tz (three ) Tj 1.905556 0 Td 55.092593 Tz (time ) Tj 1.730556 0 Td 51.203704 Tz (point ) Tj 1.944444 0 Td 41.049383 Tz (all ) Tj 1.186111 0 Td 55.092593 Tz (groups ) Tj 2.430556 0 Td 45.868946 Tz (significantly ) Tj 4.063889 0 Td 51.131687 Tz (increased ) Tj 51.428571 0 0 51.428571 0 422.432564 Tm 6.338889 0 Td 53.796296 Tz (throughout ) Tj 3.402778 0 Td 49.691358 Tz (the ) Tj 1.088889 0 Td 54.282407 Tz (example. ) Tj 2.838889 0 Td 50.555556 Tz (Sequential ) Tj 3.247222 0 Td 45.910494 Tz (levels ) Tj 1.847222 0 Td 59.953704 Tz (of ) Tj 0.797222 0 Td 54.282407 Tz (time ) Tj 1.516667 0 Td 43.75 Tz (in ) Tj 0.719444 0 Td 48.611111 Tz (culture ) Tj 51.428571 0 0 51.428571 0 376.721485 Tm 6.377778 0 Td 47.260802 Tz (\(differences ) Tj 3.888889 0 Td 57.407407 Tz (between ) Tj 2.877778 0 Td 55.092593 Tz (days ) Tj 1.808333 0 Td 47.962963 Tz (three ) Tj 1.847222 0 Td 60.493827 Tz (and ) Tj 1.536111 0 Td 49.691358 Tz (seven; ) Tj 2.275 0 Td 48.611111 Tz (differences ) Tj 51.428571 0 0 51.428571 0 337.933655 Tm 6.338889 0 Td 57.87037 Tz (between ) Tj 2.644444 0 Td 57.253086 Tz (day ) Tj 1.225 0 Td 53.796296 Tz (seven ) Tj 1.808333 0 Td 59.41358 Tz (and ) Tj 1.263889 0 Td 49.331276 Tz (fourteen\) ) Tj 2.858333 0 Td 50.636574 Tz (revealed ) Tj 2.605556 0 Td 51.311728 Tz (highly ) Tj 2.061111 0 Td 45.959596 Tz (significant ) Tj 51.428571 0 0 51.428571 0 295.96 Tm 6.358333 0 Td 49.331276 Tz (increases ) Tj 2.936111 0 Td 43.75 Tz (in ) Tj 0.816667 0 Td 84.259259 Tz (DNA ) Tj 1.769444 0 Td 46.990741 Tz (quantities ) Tj 3.091667 0 Td 54.372428 Tz (\(p<0.0001 ) Tj 3.247222 0 Td 48.611111 Tz (for ) Tj 1.069444 0 Td 47.530864 Tz (both\). ) Tj 2.022222 0 Td 52.256944 Tz (Post-hoc ) Tj 51.428571 0 0 51.428571 0 252.949821 Tm 6.338889 0 Td 46.759259 Tz (testing ) Tj 2.138889 0 Td 56.712963 Tz (of ) Tj 0.738889 0 Td 50.771605 Tz (the ) Tj 1.088889 0 Td 46.843434 Tz (interaction ) Tj 3.266667 0 Td 45.910494 Tz (levels ) Tj 1.827778 0 Td 66.759259 Tz (among ) Tj 2.158333 0 Td 48.148148 Tz (culture ) Tj 2.197222 0 Td 47.314815 Tz (Substrates ) Tj 2.994444 0 Td 61.574074 Tz (and ) Tj 51.428571 0 0 51.428571 0 211.362319 Tm 6.338889 0 Td 53.148148 Tz (times ) Tj 1.769444 0 Td 47.685185 Tz (further ) Tj 2.119444 0 Td 44.290123 Tz (clarified ) Tj 2.547222 0 Td 49.691358 Tz (the ) Tj 1.088889 0 Td 48.251029 Tz (materials ) Tj 2.819444 0 Td 45.37037 Tz (roles ) Tj 1.555556 0 Td 43.75 Tz (in ) Tj 0.7 0 Td 47.222222 Tz (altered ) Tj 2.138889 0 Td 50.771605 Tz (prolifera ) Tj 51.428571 0 0 51.428571 0 169.611794 Tm 6.338889 0 Td 47.800926 Tz (tion ) Tj 1.341667 0 Td 41.589506 Tz (rates. ) Tj 1.652778 0 Td 50.555556 Tz (Cells ) Tj 1.672222 0 Td 59.953704 Tz (on ) Tj 0.913889 0 Td 44.830247 Tz (tissue ) Tj 1.788889 0 Td 48.148148 Tz (culture ) Tj 2.177778 0 Td 44.907407 Tz (plastic ) Tj 2.080556 0 Td 60.277778 Tz (\(TCP\) ) Tj 1.944444 0 Td 59.143519 Tz (were ) Tj 1.575 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 54.282407 Tz (only ) Tj 30 0 0 30 0 2547 Tm 41.666667 0 Td 75 Tz (10 ) Tj 30 0 0 30 0 2339 Tm 41.666667 0 Td 72.222222 Tz (15 ) Tj 30 0 0 30 0 1923 Tm 41.6 0 Td 77.777778 Tz (25 ) Tj 30 0 0 30 0 1716 Tm 41.6 0 Td 80.555556 Tz (30 ) Tj 30 0 0 30 0 1507 Tm 41.6 0 Td 77.777778 Tz (35 ) Tj 30 0 0 30 0 1299 Tm 41.566667 0 Td 83.333333 Tz (40 ) Tj 30 0 0 30 0 1091 Tm 41.566667 0 Td 80.555556 Tz (45 ) Tj 30 0 0 30 0 883 Tm 41.6 0 Td 80.555556 Tz (50 ) Tj 30 0 0 30 0 675 Tm 41.6 0 Td 77.777778 Tz (55 ) Tj 30 0 0 30 0 467 Tm 41.633333 0 Td 77.777778 Tz (60 ) Tj 30 0 0 30 0 259 Tm 41.633333 0 Td 75 Tz (65 ) Tj 51.428571 0 0 51.428571 0 2969 Tm 33.6 0 Td 74.537037 Tz (14 ) Tj 51.428571 0 0 51.428571 0 2915.308789 Tm 25.297222 0 Td 56.388889 Tz (group ) Tj 1.827778 0 Td 48.611111 Tz (to ) Tj 0.758333 0 Td 53.914141 Tz (demonstrate ) Tj 3.694444 0 Td 58.333333 Tz (a ) Tj 0.505556 0 Td 45.959596 Tz (significant ) Tj 3.208333 0 Td 46.548822 Tz (interaction ) Tj 3.247222 0 Td 45.910494 Tz (effect ) Tj 1.808333 0 Td 57.87037 Tz (between ) Tj 51.428571 0 0 51.428571 0 2871.538462 Tm 25.297222 0 Td 55.092593 Tz (days ) Tj 1.497222 0 Td 48.611111 Tz (three ) Tj 1.594444 0 Td 58.333333 Tz (and ) Tj 1.225 0 Td 49.151235 Tz (seven; ) Tj 1.963889 0 Td 48.611111 Tz (differences ) Tj 3.344444 0 Td 57.87037 Tz (between ) Tj 2.625 0 Td 53.472222 Tz (time ) Tj 1.419444 0 Td 49.691358 Tz (points ) Tj 1.944444 0 Td 51.851852 Tz (within ) Tj 51.428571 0 0 51.428571 0 2827.964912 Tm 25.277778 0 Td 50.771605 Tz (the ) Tj 1.186111 0 Td 59.41358 Tz (two ) Tj 1.322222 0 Td 52.932099 Tz (experimental ) Tj 4.102778 0 Td 48.611111 Tz (material ) Tj 2.625 0 Td 47.800926 Tz (cultures ) Tj 2.586111 0 Td 58.981481 Tz (where ) Tj 2.061111 0 Td 50.771605 Tz (not ) Tj 1.205556 0 Td 50.231481 Tz (detected ) Tj 51.428571 0 0 51.428571 0 2785.491985 Tm 25.277778 0 Td 44.074074 Tz (until ) Tj 1.516667 0 Td 49.691358 Tz (the ) Tj 1.069444 0 Td 42.777778 Tz (later ) Tj 1.458333 0 Td 56.17284 Tz (day ) Tj 1.205556 0 Td 53.148148 Tz (seven ) Tj 1.788889 0 Td 45.37037 Tz (to ) Tj 0.738889 0 Td 49.826389 Tz (fourteen ) Tj 2.566667 0 Td 55.976431 Tz (comparisons ) Tj 3.85 0 Td 51.851852 Tz (\(p<0.01 ) Tj 2.469444 0 Td 47.530864 Tz (for ) Tj 51.428571 0 0 51.428571 0 2740.861575 Tm 25.277778 0 Td 47.530864 Tz (both\). ) Tj 1.886111 0 Td 75.347222 Tz (MSCs ) Tj 1.963889 0 Td 49.826389 Tz (cultured ) Tj 2.547222 0 Td 58.333333 Tz (on ) Tj 0.875 0 Td 48.611111 Tz (the ) Tj 1.030556 0 Td 52.662037 Tz (experimental ) Tj 3.966667 0 Td 51.557239 Tz (formulation ) Tj 3.597222 0 Td 67.24537 Tz (mate ) Tj 51.428571 0 0 51.428571 0 2699.444444 Tm 25.297222 0 Td 41.481481 Tz (rials ) Tj 1.4 0 Td 54.012346 Tz (demonstrated ) Tj 3.986111 0 Td 58.333333 Tz (a ) Tj 0.466667 0 Td 44.290123 Tz (partially ) Tj 2.566667 0 Td 52.824074 Tz (Suppressed ) Tj 3.266667 0 Td 47.115385 Tz (proliferation ) Tj 3.752778 0 Td 43.425926 Tz (rate, ) Tj 1.419444 0 Td 50.231481 Tz (as ) Tj 51.428571 0 0 51.428571 0 2653.026036 Tm 25.297222 0 Td 47.395833 Tz (positive ) Tj 2.391667 0 Td 49.074074 Tz (control ) Tj 2.197222 0 Td 45.37037 Tz (tissue ) Tj 1.769444 0 Td 47.685185 Tz (culture ) Tj 2.1 0 Td 44.907407 Tz (plastic ) Tj 2.061111 0 Td 60.277778 Tz (\(TCP\) ) Tj 1.925 0 Td 85.339506 Tz (DNA ) Tj 1.691667 0 Td 49.691358 Tz (values ) Tj 1.905556 0 Td 59.953704 Tz (were ) Tj 51.428571 0 0 51.428571 0 2612.794045 Tm 25.316667 0 Td 45.37037 Tz (significantly ) Tj 3.830556 0 Td 49.074074 Tz (greater ) Tj 2.294444 0 Td 51.041667 Tz (than ) Tj 1.477778 0 Td 52.662037 Tz (experimental ) Tj 4.083333 0 Td 50.771605 Tz (values ) Tj 2.080556 0 Td 46.990741 Tz (at ) Tj 0.797222 0 Td 55.902778 Tz (each ) Tj 1.575 0 Td 55.092593 Tz (time ) Tj 51.428571 0 0 51.428571 0 2565.390036 Tm 25.297222 0 Td 49.259259 Tz (point ) Tj 1.633333 0 Td 46.018519 Tz (after ) Tj 1.497222 0 Td 48.611111 Tz (three ) Tj 1.633333 0 Td 54.282407 Tz (days ) Tj 1.477778 0 Td 45.37037 Tz (in ) Tj 0.680556 0 Td 45.775463 Tz (culture. ) Tj 2.411111 0 Td 71.296296 Tz (No ) Tj 1.011111 0 Td 41.540404 Tz (statistical ) Tj 2.916667 0 Td 48.935185 Tz (difference ) Tj 3.091667 0 Td 61.574074 Tz (was ) Tj 51.428571 0 0 51.428571 0 2523.303529 Tm 25.297222 0 Td 50.636574 Tz (detected ) Tj 2.547222 0 Td 57.87037 Tz (between ) Tj 2.566667 0 Td 50.771605 Tz (the ) Tj 1.030556 0 Td 52.662037 Tz (experimental ) Tj 3.947222 0 Td 51.557239 Tz (formulation ) Tj 3.538889 0 Td 55.092593 Tz (groups ) Tj 2.138889 0 Td 43.75 Tz (at ) Tj 0.641667 0 Td 58.333333 Tz (any ) Tj 51.428571 0 0 51.428571 0 2482.675214 Tm 25.297222 0 Td 52.5 Tz (given ) Tj 1.75 0 Td 55.092593 Tz (time ) Tj 1.497222 0 Td 45.910494 Tz (point. ) Tj 51.428571 0 0 51.428571 0 2435.564496 Tm 26.016667 0 Td 75.617284 Tz (Now ) Tj 1.575 0 Td 46.8107 Tz (referring ) Tj 2.644444 0 Td 45.37037 Tz (to ) Tj 0.738889 0 Td 55.092593 Tz (FIG.3, ) Tj 2.119444 0 Td 49.691358 Tz (the ) Tj 1.030556 0 Td 49.421296 Tz (data ) Tj 1.341667 0 Td 59.62963 Tz (shows ) Tj 1.944444 0 Td 47.530864 Tz (the ) Tj 1.011111 0 Td 48.611111 Tz (fold ) Tj 1.283333 0 Td 56.018519 Tz (changes ) Tj 2.508333 0 Td 46.990741 Tz (in ) Tj 51.428571 0 0 51.428571 0 2393.669856 Tm 25.297222 0 Td 86.689815 Tz (mRNA ) Tj 2.236111 0 Td 51.527778 Tz (expression ) Tj 3.305556 0 Td 57.685185 Tz (which ) Tj 1.925 0 Td 49.691358 Tz (are ) Tj 1.05 0 Td 46.720679 Tz (collectively ) Tj 3.558333 0 Td 50.555556 Tz (suggestive ) Tj 3.227778 0 Td 55.092593 Tz (of ) Tj 0.758333 0 Td 77.777778 Tz (mes ) Tj 51.428571 0 0 51.428571 0 2348.265839 Tm 25.297222 0 Td 58.738426 Tz (enchymal ) Tj 3.111111 0 Td 55.902778 Tz (stem ) Tj 1.672222 0 Td 42.12963 Tz (cell ) Tj 1.322222 0 Td 45.802469 Tz (differentiation ) Tj 4.413889 0 Td 53.796296 Tz (along ) Tj 1.905556 0 Td 55.092593 Tz (an ) Tj 0.952778 0 Td 52.350427 Tz (osteochondral ) Tj 51.428571 0 0 51.428571 0 2307.147503 Tm 25.297222 0 Td 53.877315 Tz (pathway, ) Tj 2.741667 0 Td 43.625356 Tz (specifically, ) Tj 3.558333 0 Td 45.37037 Tz (to ) Tj 0.680556 0 Td 58.333333 Tz (immature ) Tj 2.936111 0 Td 53.148148 Tz (nonhypertrophic ) Tj 4.919444 0 Td 62.5 Tz (chondro ) Tj 51.428571 0 0 51.428571 0 2260.967273 Tm 25.297222 0 Td 45.910494 Tz (cytes; ) Tj 1.808333 0 Td 53.703704 Tz (average ) Tj 2.430556 0 Td 48.611111 Tz (fold ) Tj 1.322222 0 Td 56.712963 Tz (change ) Tj 2.197222 0 Td 66.435185 Tz (\(n2\) ) Tj 1.75 0 Td 58.333333 Tz (and ) Tj 1.225 0 Td 55.092593 Tz (+/- ) Tj 1.147222 0 Td 57.253086 Tz (one ) Tj 1.166667 0 Td 51.446759 Tz (standard ) Tj 2.625 0 Td 59.62963 Tz (devia ) Tj 51.428571 0 0 51.428571 0 2220.521127 Tm 25.277778 0 Td 46.990741 Tz (tion ) Tj 1.244444 0 Td 51.851852 Tz (as ) Tj 0.758333 0 Td 59.953704 Tz (compared ) Tj 2.975 0 Td 46.990741 Tz (to ) Tj 0.7 0 Td 56.17284 Tz (second ) Tj 2.119444 0 Td 54.62963 Tz (passage ) Tj 2.430556 0 Td 57.973251 Tz (monolayer ) Tj 3.208333 0 Td 44.290123 Tz (rat ) Tj 0.913889 0 Td 65.219907 Tz (mesenchy ) Tj 51.428571 0 0 51.428571 0 2171.858926 Tm 25.297222 0 Td 60.493827 Tz (mal ) Tj 1.302778 0 Td 55.092593 Tz (stem ) Tj 1.516667 0 Td 44.722222 Tz (cells ) Tj 1.536111 0 Td 50.771605 Tz (are ) Tj 1.088889 0 Td 56.712963 Tz (shown. ) Tj 2.313889 0 Td 49.537037 Tz (Results ) Tj 2.275 0 Td 58.333333 Tz (from ) Tj 1.613889 0 Td 49.691358 Tz (the ) Tj 1.069444 0 Td 50.462963 Tz (studied ) Tj 2.275 0 Td 55.740741 Tz (genes ) Tj 1.866667 0 Td 58.333333 Tz (of ) Tj 51.428571 0 0 51.428571 0 2129.373737 Tm 25.297222 0 Td 44.965278 Tz (interest ) Tj 2.333333 0 Td 50.771605 Tz (are ) Tj 1.127778 0 Td 61.574074 Tz (shown ) Tj 2.080556 0 Td 45.37037 Tz (in ) Tj 0.738889 0 Td 50.771605 Tz (the ) Tj 1.108333 0 Td 52.932099 Tz (bar ) Tj 1.127778 0 Td 50.925926 Tz (graphs; ) Tj 2.372222 0 Td 50.636574 Tz (collagen ) Tj 2.663889 0 Td 38.888889 Tz (II ) Tj 0.680556 0 Td 58.333333 Tz (and ) Tj 1.244444 0 Td 47.222222 Tz (osterix ) Tj 51.428571 0 0 51.428571 0 2085.568434 Tm 25.297222 0 Td 86.689815 Tz (mRNA ) Tj 2.275 0 Td 44.444444 Tz (results ) Tj 2.061111 0 Td 49.691358 Tz (are ) Tj 1.088889 0 Td 50.771605 Tz (not ) Tj 1.088889 0 Td 61.574074 Tz (shown ) Tj 2.041667 0 Td 50.231481 Tz (as ) Tj 0.797222 0 Td 44.722222 Tz (their ) Tj 1.497222 0 Td 51.527778 Tz (expression ) Tj 3.286111 0 Td 61.574074 Tz (was ) Tj 1.283333 0 Td 48.148148 Tz (absent. ) Tj 58.571429 0 0 58.571429 0 2043.834887 Tm 22.229268 0 Td 54.776423 Tz (5x10 ) Tj 1.878049 0 Td 48.373984 Tz (second ) Tj 2.065854 0 Td 47.560976 Tz (passage ) Tj 2.304878 0 Td 37.940379 Tz (rat ) Tj 1.02439 0 Td 52.512934 Tz (mesenchymal ) Tj 3.790244 0 Td 49.085366 Tz (stem ) Tj 1.502439 0 Td 39.837398 Tz (cells ) Tj 1.519512 0 Td 51.930894 Tz (were ) Tj 51.428571 0 0 51.428571 0 1999.088235 Tm 25.316667 0 Td 54.552469 Tz (seeded ) Tj 2.119444 0 Td 52.662037 Tz (onto ) Tj 1.497222 0 Td 38.888889 Tz (1 ) Tj 0.466667 0 Td 59.953704 Tz (ml ) Tj 0.894444 0 Td 56.018519 Tz (samples ) Tj 2.508333 0 Td 55.092593 Tz (of ) Tj 0.758333 0 Td 49.691358 Tz (the ) Tj 1.030556 0 Td 52.391975 Tz (experimental ) Tj 3.966667 0 Td 48.611111 Tz (materials ) Tj 2.819444 0 Td 57.523148 Tz (\(F1+ ) Tj 51.428571 0 0 51.428571 0 1953 Tm 25.297222 0 Td 58.333333 Tz (and ) Tj 1.225 0 Td 57.523148 Tz (F2+\) ) Tj 1.536111 0 Td 53.472222 Tz (or ) Tj 0.758333 0 Td 52.662037 Tz (onto ) Tj 1.438889 0 Td 51.851852 Tz (a ) Tj 0.466667 0 Td 49.074074 Tz (control ) Tj 2.255556 0 Td 48.611111 Tz (material ) Tj 2.508333 0 Td 55.092593 Tz (\(F1-\) ) Tj 1.788889 0 Td 49.074074 Tz (similar ) Tj 2.177778 0 Td 45.37037 Tz (to ) Tj 0.738889 0 Td 61.574074 Tz (F1+ ) Tj 1.302778 0 Td 54.282407 Tz (with ) Tj 51.428571 0 0 51.428571 0 1912.570732 Tm 25.277778 0 Td 55.092593 Tz (water ) Tj 1.905556 0 Td 49.691358 Tz (replacing ) Tj 2.955556 0 Td 49.691358 Tz (the ) Tj 1.147222 0 Td 51.851852 Tz (concentrated ) Tj 3.986111 0 Td 44.965278 Tz (platelet ) Tj 2.411111 0 Td 47.170782 Tz (releasate ) Tj 2.780556 0 Td 47.395833 Tz (portion. ) Tj 51.428571 0 0 51.428571 0 1866.892121 Tm 25.297222 0 Td 51.041667 Tz (Cultures ) Tj 2.625 0 Td 59.953704 Tz (were ) Tj 1.613889 0 Td 53.472222 Tz (maintained ) Tj 3.402778 0 Td 47.530864 Tz (for ) Tj 0.991667 0 Td 49.691358 Tz (the ) Tj 1.108333 0 Td 49.537037 Tz (various ) Tj 2.255556 0 Td 54.282407 Tz (time ) Tj 1.438889 0 Td 49.691358 Tz (points ) Tj 1.983333 0 Td 56.17284 Tz (shown. ) Tj 51.428571 0 0 51.428571 0 1826.278129 Tm 26.016667 0 Td 87.5 Tz (A ) Tj 0.719444 0 Td 54.444444 Tz (quick ) Tj 1.769444 0 Td 49.259259 Tz (burst ) Tj 1.633333 0 Td 58.333333 Tz (of ) Tj 0.836111 0 Td 62.87037 Tz (Runx2 ) Tj 2.061111 0 Td 51.527778 Tz (expression ) Tj 3.305556 0 Td 53.472222 Tz (followed ) Tj 2.722222 0 Td 61.574074 Tz (by ) Tj 0.933333 0 Td 57.523148 Tz (upregula ) Tj 51.428571 0 0 51.428571 0 1778.754717 Tm 25.277778 0 Td 46.990741 Tz (tion ) Tj 1.380556 0 Td 61.574074 Tz (of ) Tj 0.913889 0 Td 60.763889 Tz (Sox9 ) Tj 1.75 0 Td 85.069444 Tz (mRNA ) Tj 2.333333 0 Td 37.268519 Tz (is ) Tj 0.7 0 Td 48.935185 Tz (consistent ) Tj 3.188889 0 Td 53.472222 Tz (with ) Tj 1.536111 0 Td 48.611111 Tz (early ) Tj 1.711111 0 Td 55.092593 Tz (endochondral ) Tj 51.428571 0 0 51.428571 0 1738.497487 Tm 25.277778 0 Td 59.953704 Tz (bone ) Tj 1.75 0 Td 52.572016 Tz (formation ) Tj 3.169444 0 Td 51.851852 Tz (processes ) Tj 3.130556 0 Td 59.62963 Tz (where ) Tj 2.119444 0 Td 60.10101 Tz (mesenchymal ) Tj 4.316667 0 Td 55.092593 Tz (stem ) Tj 1.672222 0 Td 44.074074 Tz (cells ) Tj 51.428571 0 0 51.428571 0 1696.718412 Tm 25.277778 0 Td 51.041667 Tz (take ) Tj 1.438889 0 Td 58.333333 Tz (on ) Tj 0.913889 0 Td 50.771605 Tz (the ) Tj 1.147222 0 Td 52.932099 Tz (nonhypertrophic ) Tj 4.997222 0 Td 54.208754 Tz (chondrocyte ) Tj 3.772222 0 Td 53.472222 Tz (phenotype. ) Tj 3.441667 0 Td 73.726852 Tz (Main ) Tj 52.857143 0 0 52.857143 0 1648.611247 Tm 24.594595 0 Td 47.747748 Tz (taining ) Tj 2.1 0 Td 48.348348 Tz (the ) Tj 0.964865 0 Td 50.765766 Tz (expression ) Tj 3.178378 0 Td 53.603604 Tz (of ) Tj 0.718919 0 Td 59.121622 Tz (Sox9 ) Tj 1.551351 0 Td 47.297297 Tz (as ) Tj 0.7 0 Td 39.414414 Tz (is ) Tj 0.605405 0 Td 51.238739 Tz (seen ) Tj 1.362162 0 Td 45.720721 Tz (in ) Tj 0.737838 0 Td 58.858859 Tz (F2+ ) Tj 1.210811 0 Td 45.720721 Tz (cultures ) Tj 2.345946 0 Td 44.144144 Tz (in ) Tj 0.643243 0 Td 49.399399 Tz (the ) Tj 51.428571 0 0 51.428571 0 1606.546296 Tm 25.297222 0 Td 55.092593 Tz (absence ) Tj 2.45 0 Td 53.472222 Tz (of ) Tj 0.719444 0 Td 43.425926 Tz (later ) Tj 1.4 0 Td 51.311728 Tz (hypertrophic ) Tj 3.85 0 Td 52.121914 Tz (chondrocyte, ) Tj 3.85 0 Td 45.730453 Tz (articular ) Tj 2.566667 0 Td 62.5 Tz (chondro ) Tj 51.428571 0 0 51.428571 0 1559.707347 Tm 25.297222 0 Td 46.018519 Tz (cyte, ) Tj 1.613889 0 Td 58.333333 Tz (and ) Tj 1.263889 0 Td 48.935185 Tz (osteoblast ) Tj 3.188889 0 Td 54.166667 Tz (markers ) Tj 2.547222 0 Td 48.287037 Tz (\(indicated ) Tj 3.130556 0 Td 43.75 Tz (in ) Tj 0.738889 0 Td 54.444444 Tz (Table ) Tj 1.847222 0 Td 50.231481 Tz (2\) ) Tj 0.836111 0 Td 48.206019 Tz (indicate ) Tj 51.428571 0 0 51.428571 0 1518.021546 Tm 25.277778 0 Td 46.990741 Tz (that ) Tj 1.419444 0 Td 43.425926 Tz (cells ) Tj 1.594444 0 Td 50.771605 Tz (are ) Tj 1.225 0 Td 47.890947 Tz (retaining ) Tj 2.877778 0 Td 42.939815 Tz (this ) Tj 1.361111 0 Td 57.928241 Tz (immature ) Tj 3.052778 0 Td 53.472222 Tz (phenotype. ) Tj 3.558333 0 Td 49.826389 Tz (Interest ) Tj 51.428571 0 0 51.428571 0 1476.789677 Tm 25.297222 0 Td 44.830247 Tz (ingly, ) Tj 2.022222 0 Td 60.10101 Tz (mesenchymal ) Tj 4.375 0 Td 55.092593 Tz (stem ) Tj 1.730556 0 Td 42.939815 Tz (cell ) Tj 1.458333 0 Td 54.166667 Tz (markers ) Tj 2.663889 0 Td 59.143519 Tz (were ) Tj 1.788889 0 Td 52.146465 Tz (upregulated ) Tj 51.428571 0 0 51.428571 0 1430.770686 Tm 25.277778 0 Td 53.796296 Tz (throughout ) Tj 3.305556 0 Td 50.771605 Tz (the ) Tj 1.030556 0 Td 58.796296 Tz (example ) Tj 2.566667 0 Td 59.41358 Tz (due ) Tj 1.186111 0 Td 46.990741 Tz (to ) Tj 0.680556 0 Td 48.611111 Tz (the ) Tj 1.011111 0 Td 48.971193 Tz (materials ) Tj 2.780556 0 Td 53.148148 Tz (themselves ) Tj 3.325 0 Td 51.851852 Tz (but ) Tj 1.011111 0 Td 46.990741 Tz (to ) Tj 51.428571 0 0 51.428571 0 1389.110588 Tm 25.297222 0 Td 55.092593 Tz (a ) Tj 0.447222 0 Td 46.450617 Tz (lesser ) Tj 1.788889 0 Td 52.932099 Tz (degree ) Tj 2.041667 0 Td 56.712963 Tz (from ) Tj 1.516667 0 Td 49.691358 Tz (the ) Tj 1.011111 0 Td 52.572016 Tz (Suspected ) Tj 2.955556 0 Td 52.121914 Tz (fastergrowth ) Tj 3.869444 0 Td 47.530864 Tz (factor ) Tj 1.788889 0 Td 49.074074 Tz (eluting ) Tj 51.428571 0 0 51.428571 0 1345.236407 Tm 25.316667 0 Td 62.654321 Tz (F1+ ) Tj 1.263889 0 Td 51.851852 Tz (group. ) Tj 2.061111 0 Td 55.092593 Tz (Both ) Tj 1.477778 0 Td 49.691358 Tz (the ) Tj 0.991667 0 Td 52.662037 Tz (experimental ) Tj 3.947222 0 Td 48.971193 Tz (materials ) Tj 2.780556 0 Td 50.87963 Tz (containing ) Tj 3.188889 0 Td 72.916667 Tz (hCPR ) Tj 51.428571 0 0 51.428571 0 1297 Tm 25.316667 0 Td 57.523148 Tz (\(F1+ ) Tj 1.555556 0 Td 58.333333 Tz (and ) Tj 1.263889 0 Td 58.333333 Tz (F2+\) ) Tj 1.536111 0 Td 60.493827 Tz (and ) Tj 1.263889 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 49.074074 Tz (control ) Tj 2.275 0 Td 49.016204 Tz (material ) Tj 2.527778 0 Td 52.777778 Tz (without ) Tj 2.391667 0 Td 72.106481 Tz (hCPR ) Tj 1.925 0 Td 55.092593 Tz (\(F1-\) ) Tj 51.428571 0 0 51.428571 0 1256.304348 Tm 25.297222 0 Td 54.552469 Tz (demonstrated ) Tj 4.063889 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 44.444444 Tz (ability ) Tj 2.041667 0 Td 45.37037 Tz (to ) Tj 0.738889 0 Td 44.074074 Tz (alter ) Tj 1.438889 0 Td 50.771605 Tz (the ) Tj 1.05 0 Td 58.333333 Tz (gene ) Tj 1.555556 0 Td 51.262626 Tz (expressions ) Tj 3.577778 0 Td 56.712963 Tz (of ) Tj 0.777778 0 Td 63.734568 Tz (sec ) Tj 51.428571 0 0 51.428571 0 1212.863469 Tm 25.297222 0 Td 60.493827 Tz (ond ) Tj 1.263889 0 Td 54.166667 Tz (passage ) Tj 2.488889 0 Td 74.537037 Tz (MSCs ) Tj 2.002778 0 Td 45.37037 Tz (in ) Tj 0.758333 0 Td 56.388889 Tz (comparison ) Tj 3.597222 0 Td 45.37037 Tz (to ) Tj 0.758333 0 Td 54.282407 Tz (time ) Tj 1.497222 0 Td 51.041667 Tz (Zero ) Tj 1.438889 0 Td 50.771605 Tz (undifferenti ) Tj 51.428571 0 0 51.428571 0 1168.576779 Tm 25.297222 0 Td 51.041667 Tz (ated ) Tj 1.497222 0 Td 57.973251 Tz (monolayer ) Tj 3.363889 0 Td 46.450617 Tz (tissue ) Tj 1.925 0 Td 48.611111 Tz (culture ) Tj 2.313889 0 Td 44.907407 Tz (plastic ) Tj 2.177778 0 Td 60.277778 Tz (\(TCP\) ) Tj 2.1 0 Td 49.691358 Tz (reference ) Tj 2.955556 0 Td 62.654321 Tz (cul ) Tj 51.428571 0 0 51.428571 0 1128.993407 Tm 25.277778 0 Td 47.314815 Tz (tures ) Tj 1.633333 0 Td 46.018519 Tz (\(fold ) Tj 1.594444 0 Td 56.712963 Tz (change ) Tj 2.197222 0 Td 53.148148 Tz (equal ) Tj 1.788889 0 Td 43.75 Tz (to ) Tj 0.758333 0 Td 44.907407 Tz (unity\). ) Tj 51.428571 0 0 51.428571 0 1085.02 Tm 26.036111 0 Td 49.826389 Tz (Relative ) Tj 2.586111 0 Td 85.069444 Tz (mRNA ) Tj 2.216667 0 Td 46.450617 Tz (levels ) Tj 1.847222 0 Td 59.953704 Tz (of ) Tj 0.797222 0 Td 49.691358 Tz (the ) Tj 1.069444 0 Td 54.552469 Tz (master ) Tj 2.119444 0 Td 46.759259 Tz (transcriptions ) Tj 4.141667 0 Td 46.296296 Tz (factors ) Tj 51.428571 0 0 51.428571 0 1044.239583 Tm 25.297222 0 Td 58.333333 Tz (of ) Tj 1.030556 0 Td 53.045809 Tz (osteochondrogenesis ) Tj 6.494444 0 Td 55.092593 Tz (Sox9. ) Tj 2.158333 0 Td 57.253086 Tz (Runx2, ) Tj 2.469444 0 Td 60.493827 Tz (and ) Tj 1.497222 0 Td 47.222222 Tz (osterix ) Tj 2.411111 0 Td 58.333333 Tz (were ) Tj 51.428571 0 0 51.428571 0 1002.906367 Tm 25.297222 0 Td 58.738426 Tz (examined ) Tj 3.033333 0 Td 45.37037 Tz (in ) Tj 0.797222 0 Td 61.111111 Tz (Example ) Tj 2.858333 0 Td 37.268519 Tz (1. ) Tj 0.7 0 Td 61.574074 Tz (Sox9 ) Tj 1.691667 0 Td 51.527778 Tz (expression ) Tj 3.325 0 Td 50.771605 Tz (increased ) Tj 2.955556 0 Td 45.37037 Tz (in ) Tj 0.777778 0 Td 55.902778 Tz (each ) Tj 51.428571 0 0 51.428571 0 960.388206 Tm 25.297222 0 Td 49.421296 Tz (material ) Tj 2.547222 0 Td 55.740741 Tz (group ) Tj 1.827778 0 Td 55.092593 Tz (over ) Tj 1.477778 0 Td 47.530864 Tz (the ) Tj 1.030556 0 Td 37.592593 Tz (first ) Tj 1.283333 0 Td 48.611111 Tz (three ) Tj 1.613889 0 Td 55.092593 Tz (days ) Tj 1.497222 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 45.37037 Tz (culture. ) Tj 2.411111 0 Td 61.574074 Tz (F2+ ) Tj 1.283333 0 Td 57.685185 Tz (speci ) Tj 51.428571 0 0 51.428571 0 918.145655 Tm 25.297222 0 Td 63.194444 Tz (mens ) Tj 1.730556 0 Td 54.552469 Tz (demonstrated ) Tj 4.102778 0 Td 51.851852 Tz (a ) Tj 0.544444 0 Td 52.256944 Tz (dramatic ) Tj 2.761111 0 Td 57.253086 Tz (175 ) Tj 1.205556 0 Td 54.166667 Tz (average ) Tj 2.45 0 Td 49.421296 Tz (fold ) Tj 1.380556 0 Td 49.421296 Tz (increase ) Tj 2.566667 0 Td 63.194444 Tz (on ) Tj 51.428571 0 0 51.428571 0 878.261146 Tm 25.297222 0 Td 58.333333 Tz (day ) Tj 1.380556 0 Td 48.611111 Tz (three ) Tj 1.75 0 Td 60.493827 Tz (and ) Tj 1.380556 0 Td 50.925926 Tz (heavily ) Tj 2.469444 0 Td 50.084175 Tz (contributed ) Tj 3.616667 0 Td 46.990741 Tz (to ) Tj 0.894444 0 Td 54.6875 Tz (observed ) Tj 2.955556 0 Td 45.959596 Tz (significant ) Tj 51.428571 0 0 51.428571 0 837.842667 Tm 25.297222 0 Td 45.910494 Tz (effect ) Tj 2.022222 0 Td 58.333333 Tz (of ) Tj 0.991667 0 Td 48.206019 Tz (alginate ) Tj 2.683333 0 Td 55.092593 Tz (weight ) Tj 2.333333 0 Td 51.851852 Tz (percent ) Tj 2.566667 0 Td 45.37037 Tz (in ) Tj 0.913889 0 Td 50.771605 Tz (the ) Tj 1.283333 0 Td 54.012346 Tz (formal ) Tj 2.313889 0 Td 49.016204 Tz (analysis ) Tj 51.428571 0 0 51.428571 0 795.638073 Tm 25.316667 0 Td 54.282407 Tz (\(p=0.022 ) Tj 2.761111 0 Td 47.530864 Tz (factor ) Tj 1.808333 0 Td 43.75 Tz (level; ) Tj 1.711111 0 Td 57.407407 Tz (p=0.026 ) Tj 2.527778 0 Td 52.256944 Tz (post-hoc ) Tj 2.663889 0 Td 48.935185 Tz (difference ) Tj 3.033333 0 Td 57.87037 Tz (between ) Tj 2.547222 0 Td 61.574074 Tz (8 ) Tj 51.428571 0 0 51.428571 0 753.811821 Tm 25.297222 0 Td 58.333333 Tz (and ) Tj 1.205556 0 Td 64.814815 Tz (0 ) Tj 0.525 0 Td 54.552469 Tz (weight ) Tj 2.119444 0 Td 47.170782 Tz (percent\). ) Tj 2.761111 0 Td 58.693416 Tz (Remaining ) Tj 3.305556 0 Td 57.037037 Tz (above ) Tj 1.905556 0 Td 49.331276 Tz (reference ) Tj 2.838889 0 Td 85.339506 Tz (MSC ) Tj 1.711111 0 Td 60.493827 Tz (lev ) Tj 51.428571 0 0 51.428571 0 709.528117 Tm 25.297222 0 Td 44.290123 Tz (els ) Tj 0.913889 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 61.574074 Tz (Sox9 ) Tj 1.633333 0 Td 74.537037 Tz (mRNA, ) Tj 2.391667 0 Td 62.654321 Tz (F2+ ) Tj 1.244444 0 Td 59.41358 Tz (and ) Tj 1.225 0 Td 61.574074 Tz (F ) Tj 0.447222 0 Td 35.648148 Tz (1 ) Tj 0.311111 0 Td 58.333333 Tz (- ) Tj 0.505556 0 Td 50.771605 Tz (values ) Tj 1.944444 0 Td 51.446759 Tz (declined ) Tj 2.566667 0 Td 46.018519 Tz (after ) Tj 1.497222 0 Td 48.611111 Tz (the ) Tj 0.991667 0 Td 58.333333 Tz (day ) Tj 51.428571 0 0 51.428571 0 668.607748 Tm 25.277778 0 Td 49.259259 Tz (three ) Tj 1.652778 0 Td 51.203704 Tz (peak; ) Tj 1.75 0 Td 56.481481 Tz (whereas ) Tj 2.527778 0 Td 50.771605 Tz (the ) Tj 1.108333 0 Td 62.654321 Tz (F1+ ) Tj 1.302778 0 Td 56.388889 Tz (group ) Tj 1.886111 0 Td 54.552469 Tz (demonstrated ) Tj 4.083333 0 Td 56.712963 Tz (an ) Tj 0.855556 0 Td 55.555556 Tz (absence ) Tj 51.428571 0 0 51.428571 0 628.454913 Tm 25.297222 0 Td 58.333333 Tz (of ) Tj 0.991667 0 Td 60.763889 Tz (Sox9 ) Tj 1.827778 0 Td 51.851852 Tz (expression ) Tj 3.480556 0 Td 61.574074 Tz (on ) Tj 1.108333 0 Td 55.092593 Tz (days ) Tj 1.711111 0 Td 53.796296 Tz (seven ) Tj 2.002778 0 Td 58.333333 Tz (and ) Tj 1.438889 0 Td 47.890947 Tz (fourteen. ) Tj 3.013889 0 Td 62.222222 Tz (Runx2 ) Tj 51.428571 0 0 51.428571 0 585.614124 Tm 25.297222 0 Td 51.851852 Tz (expression ) Tj 3.363889 0 Td 47.530864 Tz (for ) Tj 1.127778 0 Td 61.574074 Tz (F1+ ) Tj 1.341667 0 Td 60.493827 Tz (and ) Tj 1.361111 0 Td 61.574074 Tz (F2+ ) Tj 1.380556 0 Td 47.800926 Tz (cultures ) Tj 2.566667 0 Td 50.771605 Tz (increased ) Tj 2.975 0 Td 54.282407 Tz (over ) Tj 1.536111 0 Td 75.347222 Tz (MSCs ) Tj 51.428571 0 0 51.428571 0 545.630689 Tm 25.297222 0 Td 58.333333 Tz (and ) Tj 1.283333 0 Td 57.79321 Tz (peaked ) Tj 2.313889 0 Td 46.990741 Tz (at ) Tj 0.797222 0 Td 58.333333 Tz (day ) Tj 1.322222 0 Td 58.333333 Tz (one ) Tj 1.283333 0 Td 59.41358 Tz (and ) Tj 1.322222 0 Td 45.37037 Tz (five ) Tj 1.322222 0 Td 50.231481 Tz (hours, ) Tj 2.080556 0 Td 46.866097 Tz (respectively; ) Tj 3.947222 0 Td 35.648148 Tz (it ) Tj 0.680556 0 Td 61.574074 Tz (was ) Tj 40 0 0 40 0 509 Tm 32.5 0 Td 65.972222 Tz (undetectable ) Tj 5.15 0 Td 56.25 Tz (in ) Tj 1.025 0 Td 57.5 Tz (cells ) Tj 2.125 0 Td 64.0625 Tz (cultured ) Tj 3.45 0 Td 75 Tz (on ) Tj 1.3 0 Td 62.5 Tz (the ) Tj 1.525 0 Td 80.555556 Tz (F1- ) Tj 1.8 0 Td 63.690476 Tz (control ) Tj 3.1 0 Td 62.5 Tz (material ) Tj 51.428571 0 0 51.428571 0 463.743621 Tm 25.277778 0 Td 53.796296 Tz (throughout ) Tj 3.402778 0 Td 50.771605 Tz (the ) Tj 1.127778 0 Td 58.796296 Tz (example ) Tj 2.683333 0 Td 58.333333 Tz (and ) Tj 1.283333 0 Td 43.75 Tz (in ) Tj 0.758333 0 Td 52.662037 Tz (experimental ) Tj 4.025 0 Td 48.971193 Tz (materials ) Tj 2.858333 0 Td 46.018519 Tz (after ) Tj 51.428571 0 0 51.428571 0 420.569636 Tm 25.297222 0 Td 51.041667 Tz (peaking. ) Tj 2.741667 0 Td 45.37037 Tz (In ) Tj 0.777778 0 Td 48.206019 Tz (contrast ) Tj 2.488889 0 Td 48.611111 Tz (to ) Tj 0.816667 0 Td 62.384259 Tz (Sox9 ) Tj 1.691667 0 Td 46.8107 Tz (findings, ) Tj 2.780556 0 Td 55.092593 Tz (F1 ) Tj 0.738889 0 Td 64.814815 Tz (+ ) Tj 0.641667 0 Td 48.611111 Tz (material ) Tj 2.586111 0 Td 73.240741 Tz (demon ) Tj 51.428571 0 0 51.428571 0 379.674362 Tm 25.316667 0 Td 45.833333 Tz (strated ) Tj 2.080556 0 Td 51.851852 Tz (a ) Tj 0.505556 0 Td 49.826389 Tz (superior ) Tj 2.547222 0 Td 43.981481 Tz (ability ) Tj 2.041667 0 Td 43.75 Tz (to ) Tj 0.719444 0 Td 54.012346 Tz (induce ) Tj 2.158333 0 Td 62.222222 Tz (Runx2 ) Tj 2.061111 0 Td 86.689815 Tz (mRNA ) Tj 2.236111 0 Td 51.851852 Tz (expression ) Tj 51.428571 0 0 51.428571 0 338.537061 Tm 25.297222 0 Td 45.37037 Tz (at ) Tj 0.836111 0 Td 47.530864 Tz (8.3 ) Tj 1.186111 0 Td 53.703704 Tz (average ) Tj 2.566667 0 Td 48.611111 Tz (fold ) Tj 1.477778 0 Td 46.450617 Tz (increase. ) Tj 2.819444 0 Td 51.388889 Tz (Osterix ) Tj 2.469444 0 Td 51.203704 Tz (expression ) Tj 3.402778 0 Td 61.574074 Tz (was ) Tj 1.419444 0 Td 53.472222 Tz (only ) Tj 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51.428571 0 0 51.428571 0 211.007194 Tm 25.316667 0 Td 56.712963 Tz (sample ) Tj 2.197222 0 Td 48.251029 Tz (reactions ) Tj 2.761111 0 Td 48.611111 Tz (\(data ) Tj 1.633333 0 Td 50.771605 Tz (not ) Tj 1.069444 0 Td 53.703704 Tz (shown\). ) Tj 2.430556 0 Td 57.685185 Tz (Given ) Tj 1.866667 0 Td 50.771605 Tz (the ) Tj 1.05 0 Td 49.537037 Tz (limited ) Tj 2.255556 0 Td 61.574074 Tz (number ) Tj 48.571429 0 0 48.571429 0 170 Tm 26.785294 0 Td 61.764706 Tz (of ) Tj 0.802941 0 Td 54.044118 Tz (observations ) Tj 4.076471 0 Td 49.754902 Tz (in ) Tj 0.823529 0 Td 51.470588 Tz (the ) Tj 1.132353 0 Td 66.568627 Tz (Runx2 ) Tj 2.182353 0 Td 62.908497 Tz (and ) Tj 1.317647 0 Td 50 Tz (osterix ) Tj 2.326471 0 Td 56.372549 Tz (models, ) Tj 2.573529 0 Td 65.196078 Tz (n=7 ) Tj 1.358824 0 Td 62.908497 Tz (and ) Tj 1.4 0 Td 39.460784 Tz (1, ) Tj ET Q endstream endobj 464 0 obj << /Length 1 >> stream q endstream endobj 465 0 obj << /Length 37798 >> stream Q q 0.24 0 0 0.24 0 0 cm BT /F1 1 Tf 3 Tr 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(was ) Tj 1.283333 0 Td 52.441077 Tz (upregulated ) Tj 51.428571 0 0 51.428571 0 2083.140025 Tm 6.358333 0 Td 61.574074 Tz (on ) Tj 0.894444 0 Td 53.240741 Tz (average ) Tj 2.372222 0 Td 57.87037 Tz (between ) Tj 2.663889 0 Td 44.290123 Tz (1.5 ) Tj 0.972222 0 Td 59.41358 Tz (and ) Tj 1.244444 0 Td 50.771605 Tz (2.0 ) Tj 1.069444 0 Td 47.800926 Tz (fold ) Tj 1.302778 0 Td 54.282407 Tz (over ) Tj 1.438889 0 Td 75.347222 Tz (MSCs ) Tj 1.944444 0 Td 46.990741 Tz (at ) Tj 0.719444 0 Td 44.560185 Tz (five ) Tj 1.225 0 Td 53.796296 Tz (hours ) Tj 51.428571 0 0 51.428571 0 2042.140127 Tm 6.358333 0 Td 58.333333 Tz (and ) Tj 1.302778 0 Td 55.092593 Tz (day ) Tj 1.244444 0 Td 51.041667 Tz (one; ) Tj 1.477778 0 Td 46.018519 Tz (after ) Tj 1.594444 0 Td 53.472222 Tz (which, ) Tj 2.158333 0 Td 51.527778 Tz (expression ) Tj 3.344444 0 Td 51.041667 Tz (declined ) Tj 2.702778 0 Td 43.75 Tz (to ) Tj 0.758333 0 Td 57.685185 Tz (below ) Tj 2.002778 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 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Tz (highly ) Tj 2.119444 0 Td 47.800926 Tz (variable ) Tj 2.527778 0 Td 45.37037 Tz (at ) Tj 0.758333 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 1875.301144 Tm 6.358333 0 Td 45.37037 Tz (five ) Tj 1.244444 0 Td 56.712963 Tz (hour ) Tj 1.516667 0 Td 59.41358 Tz (and ) Tj 1.263889 0 Td 56.17284 Tz (day ) Tj 1.186111 0 Td 59.41358 Tz (one ) Tj 1.244444 0 Td 54.282407 Tz (time ) Tj 1.477778 0 Td 49.259259 Tz (point ) Tj 1.672222 0 Td 48.611111 Tz (for ) Tj 0.991667 0 Td 39.969136 Tz (all ) Tj 0.933333 0 Td 53.148148 Tz (\(F1+, ) Tj 1.808333 0 Td 53.472222 Tz (F2+, ) Tj 1.477778 0 Td 59.41358 Tz (and ) Tj 1.263889 0 Td 57.523148 Tz (F1-\) ) Tj 51.428571 0 0 51.428571 0 1835.912258 Tm 6.358333 0 Td 58.333333 Tz (and ) Tj 1.438889 0 Td 52.391975 Tz (experimental ) Tj 4.180556 0 Td 57.523148 Tz (\(F1+ ) Tj 1.769444 0 Td 59.41358 Tz (and ) Tj 1.458333 0 Td 57.523148 Tz (F2+\) ) Tj 1.788889 0 Td 46.990741 Tz (materials, ) Tj 3.208333 0 Td 46.118234 Tz (respectively. ) Tj 51.428571 0 0 51.428571 0 1795.624113 Tm 6.358333 0 Td 59.143519 Tz (Complete ) Tj 3.013889 0 Td 46.990741 Tz (inhibition ) Tj 3.013889 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 48.611111 Tz (alkaline ) Tj 2.508333 0 Td 53.914141 Tz (phosphatase ) Tj 3.713889 0 Td 51.851852 Tz (expression ) Tj 3.344444 0 Td 60.493827 Tz (was ) Tj 51.428571 0 0 51.428571 0 1750.318681 Tm 6.377778 0 Td 52.662037 Tz (seen ) Tj 1.380556 0 Td 46.018519 Tz (after ) Tj 1.497222 0 Td 55.092593 Tz (5 ) Tj 0.466667 0 Td 53.796296 Tz (hours ) Tj 1.769444 0 Td 47.530864 Tz (for ) Tj 0.972222 0 Td 56.712963 Tz (F1 ) Tj 0.777778 0 Td 64.814815 Tz (- ) Tj 0.505556 0 Td 48.611111 Tz (control ) Tj 2.216667 0 Td 49.016204 Tz (material ) Tj 2.488889 0 Td 50.771605 Tz (but ) Tj 1.011111 0 Td 48.611111 Tz (persisted ) Tj 2.761111 0 Td 50.771605 Tz (out ) Tj 1.030556 0 Td 46.990741 Tz (to ) Tj 51.428571 0 0 51.428571 0 1712.923858 Tm 6.338889 0 Td 50.771605 Tz (the ) Tj 1.186111 0 Td 58.333333 Tz (day ) Tj 1.322222 0 Td 58.333333 Tz (one ) Tj 1.361111 0 Td 54.282407 Tz (time ) Tj 1.555556 0 Td 49.907407 Tz (point ) Tj 1.769444 0 Td 48.611111 Tz (for ) Tj 1.127778 0 Td 52.391975 Tz (experimental ) Tj 4.063889 0 Td 72.916667 Tz (hCPR ) Tj 2.002778 0 Td 51.851852 Tz (containing ) Tj 51.428571 0 0 51.428571 0 1671.272727 Tm 6.358333 0 Td 46.990741 Tz (materials. ) Tj 2.994444 0 Td 58.333333 Tz (Aggrecan ) Tj 2.955556 0 Td 58.333333 Tz (gene ) Tj 1.536111 0 Td 51.527778 Tz (expression ) Tj 3.305556 0 Td 66.975309 Tz (may ) Tj 1.380556 0 Td 56.712963 Tz (have ) Tj 1.516667 0 Td 56.712963 Tz (been ) Tj 1.555556 0 Td 61.574074 Tz (upregu ) Tj 52.857143 0 0 52.857143 0 1623.84058 Tm 6.186486 0 Td 46.036036 Tz (lated ) Tj 1.494595 0 Td 45.720721 Tz (in ) Tj 0.643243 0 Td 50.45045 Tz (the ) Tj 1.059459 0 Td 53.603604 Tz (F1-material ) Tj 3.651351 0 Td 54.864865 Tz (group ) Tj 1.759459 0 Td 45.720721 Tz (at ) Tj 0.681081 0 Td 43.355856 Tz (five ) Tj 1.135135 0 Td 52.342342 Tz (hours ) Tj 1.702703 0 Td 44.144144 Tz (in ) Tj 0.681081 0 Td 46.846847 Tz (culture ) Tj 2.081081 0 Td 49.399399 Tz (but ) Tj 1.002703 0 Td 60.960961 Tz (was ) Tj 51.428571 0 0 51.428571 0 1586.914729 Tm 6.377778 0 Td 53.148148 Tz (Suppressed ) Tj 3.363889 0 Td 45.37037 Tz (in ) Tj 0.719444 0 Td 41.049383 Tz (all ) Tj 0.933333 0 Td 54.552469 Tz (groups ) Tj 2.158333 0 Td 46.018519 Tz (after ) Tj 1.536111 0 Td 56.17284 Tz (day ) Tj 1.205556 0 Td 50.231481 Tz (one. ) Tj 51.428571 0 0 51.428571 0 1544.497462 Tm 7.097222 0 Td 48.611111 Tz (Additionally, ) Tj 4.083333 0 Td 48.611111 Tz (the ) Tj 1.186111 0 Td 56.565657 Tz (MSC-related ) Tj 4.005556 0 Td 50 Tz (surface ) Tj 2.372222 0 Td 46.585648 Tz (integrin ) Tj 2.527778 0 Td 53.877315 Tz (endoglin ) Tj 51.428571 0 0 51.428571 0 1504.625442 Tm 6.377778 0 Td 57.118056 Tz (\(CD105\), ) Tj 2.936111 0 Td 48.611111 Tz (fibrogenic ) Tj 3.091667 0 Td 49.074074 Tz (protein ) Tj 2.255556 0 Td 59.953704 Tz (S100a4 ) Tj 2.352778 0 Td 45.208333 Tz (\(fibroblast-specific ) Tj 5.6 0 Td 66.975309 Tz (pro ) Tj 51.428571 0 0 51.428571 0 1462.955659 Tm 6.338889 0 Td 46.180556 Tz (tein ) Tj 1.302778 0 Td 49.537037 Tz (\(FSP\)\), ) Tj 2.255556 0 Td 59.41358 Tz (and ) Tj 1.225 0 Td 53.009259 Tz (glyceraldehyde ) Tj 4.608333 0 Td 54.208754 Tz (3-phosphate ) Tj 3.733333 0 Td 56.089744 Tz (dehydrogenase ) Tj 51.428571 0 0 51.428571 0 1418.417928 Tm 6.377778 0 Td 70.833333 Tz (\(GAPDH\) ) Tj 3.111111 0 Td 85.87963 Tz (mRNA ) Tj 2.177778 0 Td 51.262626 Tz (expressions ) Tj 3.519444 0 Td 58.333333 Tz (were ) Tj 1.555556 0 Td 52.175926 Tz (monitored. ) Tj 3.305556 0 Td 44.675926 Tz (Interestingly, ) Tj 51.428571 0 0 51.428571 0 1377.887668 Tm 6.358333 0 Td 69.351852 Tz (CD105 ) Tj 2.313889 0 Td 85.87963 Tz (mRNA ) Tj 2.236111 0 Td 61.574074 Tz (was ) Tj 1.322222 0 Td 56.388889 Tz (found ) Tj 1.866667 0 Td 45.37037 Tz (to ) Tj 0.738889 0 Td 58.333333 Tz (be ) Tj 0.894444 0 Td 45.619658 Tz (significantly ) Tj 3.752778 0 Td 52.146465 Tz (upregulated ) Tj 3.597222 0 Td 61.574074 Tz (by ) Tj 51.428571 0 0 51.428571 0 1334.966184 Tm 6.338889 0 Td 50.771605 Tz (the ) Tj 1.030556 0 Td 53.472222 Tz (presence ) Tj 2.702778 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 72.106481 Tz (hCPR ) Tj 1.905556 0 Td 51.557239 Tz (\(p<0.0001\), ) Tj 3.538889 0 Td 60.493827 Tz (and ) Tj 1.244444 0 Td 56.712963 Tz (no ) Tj 0.816667 0 Td 46.450617 Tz (effect ) Tj 1.827778 0 Td 60.493827 Tz (was ) Tj 1.263889 0 Td 56.388889 Tz (found ) Tj 1.827778 0 Td 48.611111 Tz (in ) Tj 51.428571 0 0 51.428571 0 1295.209567 Tm 6.338889 0 Td 50.771605 Tz (the ) Tj 1.127778 0 Td 66.975309 Tz (FSP ) Tj 1.341667 0 Td 58.333333 Tz (gene ) Tj 1.575 0 Td 51.527778 Tz (expression ) Tj 3.325 0 Td 53.472222 Tz (model. ) Tj 38.571429 0 0 38.571429 0 1211 Tm 18.251852 0 Td 101.111111 Tz (TABLE ) Tj 3.266667 0 Td 77.777778 Tz (2 ) Tj 41.428571 0 0 41.428571 0 1140.685796 Tm 9.437931 0 Td 63.793103 Tz (Summary ) Tj 2.872414 0 Td 56.321839 Tz (of ) Tj 0.796552 0 Td 71.072797 Tz (PCR ) Tj 1.52069 0 Td 45.59387 Tz (reactions ) Tj 2.655172 0 Td 48.850575 Tz (applied ) Tj 2.244828 0 Td 44.252874 Tz (to ) Tj 0.748276 0 Td 73.41954 Tz (cDNA ) Tj 1.97931 0 Td 55.316092 Tz (from ) Tj 1.544828 0 Td 52.298851 Tz (each ) Tj 1.448276 0 Td 51.851852 Tz (specimen. ) Tj 30 0 0 30 0 1078 Tm 10.9 0 Td 86.111111 Tz (Gene ) Tj 37.142857 0 0 37.142857 0 1044.006623 Tm 8.830769 0 Td 67.307692 Tz (Symbol ) Tj 3.15 0 Td 53.846154 Tz (Alias ) Tj 7 0 Td 59.82906 Tz (Refseq ) Tj 2.396154 0 Td 67.307692 Tz (# ) Tj 3.096154 0 Td 48.798077 Tz (Official ) Tj 2.638462 0 Td 67.86859 Tz (FullName ) Tj 35.918367 0 0 35.918367 0 981.821555 Tm 9.131818 0 Td 70.530303 Tz (Runx2 ) Tj 3.229545 0 Td 63.106061 Tz (Cbfa1 ) Tj 7.238636 0 Td 113.683712 Tz (XM ) Tj 1.726136 0 Td 68.055556 Tz (346O16 ) Tj 3.98125 0 Td 67.282197 Tz (Runt ) Tj 1.781818 0 Td 52.367424 Tz (related ) Tj 2.394318 0 Td 52.82634 Tz (transcription ) Tj 31.428571 0 0 31.428571 0 951 Tm 28.922727 0 Td 60.984848 Tz (factor ) Tj 2.386364 0 Td 74.242424 Tz (2 ) Tj 37.959184 0 0 37.959184 0 913.679435 Tm 8.64086 0 Td 56.200717 Tz (Sparc ) Tj 3.02957 0 Td 140.501792 Tz ( ) Tj 6.902151 0 Td 105.376344 Tz (NM ) Tj 1.633333 0 Td 63.664875 Tz (012656 ) Tj 3.767204 0 Td 54.334677 Tz (Secreted ) Tj 2.792473 0 Td 49.395161 Tz (protein, ) Tj 2.58172 0 Td 47.670251 Tz (acidic, ) Tj 40 0 0 40 0 884 Tm 22.725 0 Td 47.75641 Tz (cysteine-rich ) Tj 40 0 0 40 0 845 Tm 22.75 0 Td 48.076923 Tz (\(Osteonectin\) ) Tj 36.734694 0 0 36.734694 0 816.317526 Tm 8.928889 0 Td 66.54321 Tz (Sp7 ) Tj 3.157778 0 Td 71.080247 Tz (OSX ) Tj 7.105 0 Td 108.888889 Tz (NM ) Tj 1.687778 0 Td 66.54321 Tz (181374 ) Tj 3.892778 0 Td 66.54321 Tz (Sp7 ) Tj 1.415556 0 Td 50.954416 Tz (transcription ) Tj 4.219444 0 Td 51.419753 Tz (factor ) Tj 40 0 0 40 0 781.859112 Tm 8.15 0 Td 55.208333 Tz (Alpl ) Tj 2.925 0 Td 64.880952 Tz (Akp2/MGC93545, ) Tj 6.575 0 Td 95.833333 Tz (NM ) Tj 1.55 0 Td 59.722222 Tz (013059 ) Tj 3.5 0 Td 52.604167 Tz (Alkaline ) Tj 2.725 0 Td 51.736111 Tz (phosphatase, ) Tj 36.666667 0 0 36.666667 0 749.94382 Tm 12.163636 0 Td 86.363636 Tz (PHOA ) Tj 12.845455 0 Td 53.409091 Tz (iver ) Tj 1.418182 0 Td 59.504132 Tz (bone/kidney ) Tj 39.52381 0 0 39.52381 0 715.97153 Tm 8.273494 0 Td 58.192771 Tz (Bglap ) Tj 2.985542 0 Td 58.473896 Tz (Bglap2/Bgp/Bgpr ) Tj 6.578313 0 Td 96.987952 Tz (NM ) Tj 1.593976 0 Td 61.144578 Tz (013414 ) Tj 3.693976 0 Td 60.090361 Tz (Bone ) Tj 1.593976 0 Td 80.120482 Tz (gamma ) Tj 39.52381 0 0 39.52381 0 685 Tm 11.259036 0 Td 59.036145 Tz (Bgpra ) Tj 11.739759 0 Td 55.082831 Tz (carboxyglutamate ) Tj 5.490361 0 Td 44.698795 Tz (\(gla\) ) Tj 31.428571 0 0 31.428571 0 656 Tm 29.018182 0 Td 60.606061 Tz (protein ) Tj 38.095238 0 0 38.095238 0 615.819364 Tm 8.58375 0 Td 59.791667 Tz (Col2a1 ) Tj 3.07125 0 Td 72.355769 Tz (CG2A1AfCOLLII ) Tj 6.8775 0 Td 102.8125 Tz (NM ) Tj 1.60125 0 Td 63.4375 Tz (012929 ) Tj 3.7275 0 Td 54.444444 Tz (Collagen, ) Tj 3.15 0 Td 54.6875 Tz (type ) Tj 1.5225 0 Td 37.916667 Tz (II, ) Tj 0.84 0 Td 55.125 Tz (alpha ) Tj 1.91625 0 Td 43.75 Tz (1 ) Tj 36.825397 0 0 36.825397 0 584.012324 Tm 8.879741 0 Td 61.853448 Tz (Col1a1 ) Tj 3.20431 0 Td 74.676724 Tz (COLLA1 ) Tj 7.03319 0 Td 110.883621 Tz (XM ) Tj 1.737931 0 Td 64.87069 Tz (213440 ) Tj 3.828879 0 Td 56.321839 Tz (Collagen, ) Tj 3.258621 0 Td 56.573276 Tz (type ) Tj 1.575 0 Td 36.206897 Tz (I, ) Tj 0.597414 0 Td 57.931034 Tz (alpha ) Tj 2.009483 0 Td 45.258621 Tz (1 ) Tj 36.190476 0 0 36.190476 0 550.051522 Tm 9.007895 0 Td 71.381579 Tz (Acan ) Tj 3.232895 0 Td 62.171053 Tz (Agci ) Tj 1.575 0 Td 70.230263 Tz (Agc1 ) Tj 5.664474 0 Td 110.526316 Tz (NM ) Tj 1.713158 0 Td 66.776316 Tz (022190 ) Tj 3.896053 0 Td 66.200658 Tz (Aggrecan ) Tj 37.714286 0 0 37.714286 0 515.79668 Tm 8.670455 0 Td 69.234007 Tz (Eng ) Tj 3.102273 0 Td 75.126263 Tz (MGC124909 ) Tj 6.893939 0 Td 106.060606 Tz (NM ) Tj 1.69697 0 Td 63.341751 Tz (001010968 ) Tj 3.791667 0 Td 58.001894 Tz (Endoglin ) Tj 36.428571 0 0 36.428571 0 482.84 Tm 9.003922 0 Td 66.339869 Tz (Sox9 ) Tj 10.321569 0 Td 112.091503 Tz (XM ) Tj 1.729412 0 Td 64.814815 Tz (343981 ) Tj 3.92549 0 Td 67.973856 Tz (SRY-box ) Tj 3.047059 0 Td 56.732026 Tz (containing ) Tj 3.568627 0 Td 62.908497 Tz (gene ) Tj 30 0 0 30 0 452 Tm 30.3 0 Td 77.777778 Tz (9 ) Tj 37.142857 0 0 37.142857 0 415.588496 Tm 8.830769 0 Td 65.064103 Tz (S100a4 ) Tj 3.176923 0 Td 71.794872 Tz (18A2.42ACAPL, ) Tj 6.973077 0 Td 107.692308 Tz (NM ) Tj 1.696154 0 Td 64.316239 Tz (012618 ) Tj 3.823077 0 Td 65.064103 Tz (S100 ) Tj 1.776923 0 Td 57.136752 Tz (calcium-binding ) Tj 31.428571 0 0 31.428571 0 386.234375 Tm 14.127273 0 Td 94.128788 Tz (MTS1 ) Tj 2.704545 0 Td 70.265152 Tz (P9ka ) Tj 2.1 0 Td 71.590909 Tz (PEL98. ) Tj 10.086364 0 Td 59.848485 Tz (protein ) Tj 2.704545 0 Td 95.454545 Tz (A4 ) Tj 30 0 0 30 0 352 Tm 14.8 0 Td 104.62963 Tz (RNP9KA ) Tj 35 0 0 35 0 316.219941 Tm 9.314286 0 Td 67.857143 Tz (Actb ) Tj 3.342857 0 Td 69.047619 Tz (ActX ) Tj 7.457143 0 Td 116.666667 Tz (NM ) Tj 1.828571 0 Td 69.047619 Tz (031144 ) Tj 4 0 Td 57.936508 Tz (Actin, ) Tj 2.514286 0 Td 80.952381 Tz (3 ) Tj 37.714286 0 0 37.714286 0 284.176829 Tm 8.670455 0 Td 68.055556 Tz (Gapdh ) Tj 3.102273 0 Td 69.602273 Tz (Gapd ) Tj 6.920455 0 Td 106.060606 Tz (NM ) Tj 1.643939 0 Td 64.078283 Tz (O17008 ) Tj 3.765152 0 Td 56.409685 Tz (Glyceraldehyde-3- ) Tj 40 0 0 40 0 252 Tm 22.8 0 Td 53.240741 Tz (phosphate ) Tj 40 0 0 40 0 219 Tm 22.725 0 Td 56.089744 Tz (dehydrogenase ) Tj 30 0 0 30 0 2547 Tm 41.666667 0 Td 75 Tz (10 ) Tj 30 0 0 30 0 2339 Tm 41.666667 0 Td 72.222222 Tz (15 ) Tj 30 0 0 30 0 1923 Tm 41.6 0 Td 77.777778 Tz (25 ) Tj 30 0 0 30 0 1716 Tm 41.6 0 Td 80.555556 Tz (30 ) Tj 30 0 0 30 0 1507 Tm 41.6 0 Td 77.777778 Tz (35 ) Tj 30 0 0 30 0 1299 Tm 41.566667 0 Td 83.333333 Tz (40 ) Tj 30 0 0 30 0 1091 Tm 41.566667 0 Td 80.555556 Tz (45 ) Tj 30 0 0 30 0 883 Tm 41.6 0 Td 80.555556 Tz (50 ) Tj 30 0 0 30 0 675 Tm 41.6 0 Td 77.777778 Tz (55 ) Tj 30 0 0 30 0 467 Tm 41.633333 0 Td 77.777778 Tz (60 ) Tj 30 0 0 30 0 259 Tm 41.633333 0 Td 75 Tz (65 ) Tj 52.857143 0 0 52.857143 0 2968 Tm 32.691892 0 Td 72.522523 Tz (16 ) Tj 40 0 0 40 0 2913 Tm 39.975 0 Td 98.333333 Tz (TABLE ) Tj 3.175 0 Td 68.181818 Tz (2-continued ) Tj 40 0 0 40 0 2842.684892 Tm 34.15 0 Td 66.666667 Tz (Summary ) Tj 3 0 Td 54.166667 Tz (of ) Tj 0.825 0 Td 73.611111 Tz (PCR ) Tj 1.55 0 Td 47.685185 Tz (reactions ) Tj 2.8 0 Td 50 Tz (applied ) Tj 2.3 0 Td 45.833333 Tz (to ) Tj 0.775 0 Td 77.083333 Tz (cDNA ) Tj 2.05 0 Td 57.291667 Tz (from ) Tj 1.6 0 Td 53.125 Tz (each ) Tj 1.5 0 Td 53.240741 Tz (Specimen. ) Tj 30 0 0 30 0 2780 Tm 43.366667 0 Td 84.722222 Tz (Gene ) Tj 37.142857 0 0 37.142857 0 2746.004425 Tm 35.053846 0 Td 66.559829 Tz (Symbol ) Tj 3.123077 0 Td 53.846154 Tz (Alias ) Tj 7.026923 0 Td 59.82906 Tz (Refseq ) Tj 2.369231 0 Td 67.307692 Tz (# ) Tj 3.123077 0 Td 48.798077 Tz (Official ) Tj 2.638462 0 Td 67.86859 Tz (FullName ) Tj 30.714286 0 0 30.714286 0 2685 Tm 42.35814 0 Td 108.527132 Tz (RGDC ) Tj 3.776744 0 Td 109.883721 Tz (RGDC ) Tj 8.562791 0 Td 83.204134 Tz (U26919 ) Tj 6.64186 0 Td 72.351421 Tz (Rat ) Tj 1.562791 0 Td 81.395349 Tz (Genomic ) Tj 3.711628 0 Td 112.144703 Tz (DNA ) Tj 31.428571 0 0 31.428571 0 2652 Tm 59.913636 0 Td 70.571096 Tz (Contamination ) Tj 34.761905 0 0 34.761905 0 2617.10661 Tm 37.426027 0 Td 86.30137 Tz (RTC ) Tj 3.336986 0 Td 87.899543 Tz (RTC ) Tj 7.565753 0 Td 86.30137 Tz (SA ) Tj 1.438356 0 Td 70.958904 Tz (OO104 ) Tj 4.40137 0 Td 63.69863 Tz (Reverse ) Tj 2.847945 0 Td 57.903056 Tz (Transcription ) Tj 31.428571 0 0 31.428571 0 2585 Tm 59.913636 0 Td 67.424242 Tz (Control ) Tj 31.836735 0 0 31.836735 0 2551.268623 Tm 40.896154 0 Td 87.250712 Tz (PPC ) Tj 3.64359 0 Td 88.995726 Tz (PPC ) Tj 8.229487 0 Td 94.230769 Tz (SA ) Tj 1.570513 0 Td 75.384615 Tz (OO103 ) Tj 4.86859 0 Td 59.548611 Tz (Positive ) Tj 3.109615 0 Td 95.975783 Tz (PCR ) Tj 1.916026 0 Td 65.811966 Tz (Control ) Tj 51.428571 0 0 51.428571 0 2430.310174 Tm 26.036111 0 Td 46.866097 Tz (Proliferation ) Tj 3.811111 0 Td 49.421296 Tz (data ) Tj 1.322222 0 Td 60.493827 Tz (was ) Tj 1.225 0 Td 53.472222 Tz (analyzed ) Tj 2.722222 0 Td 52.662037 Tz (with ) Tj 1.4 0 Td 62.222222 Tz (JMPR\) ) Tj 2.022222 0 Td 43.013468 Tz (Statistical ) Tj 3.013889 0 Td 66.975309 Tz (Dis ) Tj 51.428571 0 0 51.428571 0 2386.826531 Tm 25.297222 0 Td 54.012346 Tz (covery ) Tj 2.255556 0 Td 54.282407 Tz (Software ) Tj 2.916667 0 Td 64.00463 Tz (\(SAS ) Tj 1.866667 0 Td 42.12963 Tz (Institute; ) Tj 2.838889 0 Td 51.203704 Tz (Cary, ) Tj 1.827778 0 Td 49.151235 Tz (N.C.\). ) Tj 2.119444 0 Td 84.259259 Tz (DNA ) Tj 1.827778 0 Td 50.231481 Tz (values ) Tj 51.428571 0 0 51.428571 0 2345.706161 Tm 25.277778 0 Td 59.953704 Tz (were ) Tj 1.555556 0 Td 50.771605 Tz (subjected ) Tj 2.838889 0 Td 46.990741 Tz (to ) Tj 0.7 0 Td 51.851852 Tz (a ) Tj 0.466667 0 Td 41.319444 Tz (full ) Tj 1.127778 0 Td 44.290123 Tz (factorial ) Tj 2.527778 0 Td 59.722222 Tz (two-way ) Tj 2.625 0 Td 49.016204 Tz (analysis ) Tj 2.469444 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 50.636574 Tz (variance ) Tj 51.428571 0 0 51.428571 0 2298 Tm 25.316667 0 Td 48.251029 Tz (\(C.-0.05\) ) Tj 2.761111 0 Td 43.75 Tz (to ) Tj 0.661111 0 Td 48.611111 Tz (detect ) Tj 1.866667 0 Td 45.37037 Tz (effects ) Tj 2.061111 0 Td 53.472222 Tz (of ) Tj 0.738889 0 Td 48.148148 Tz (culture ) Tj 2.138889 0 Td 46.018519 Tz (substrate, ) Tj 2.897222 0 Td 54.282407 Tz (time ) Tj 1.419444 0 Td 46.990741 Tz (in ) Tj 0.7 0 Td 45.37037 Tz (culture, ) Tj 51.428571 0 0 51.428571 0 2257.16687 Tm 25.297222 0 Td 58.333333 Tz (and ) Tj 1.244444 0 Td 45.37037 Tz (their ) Tj 1.536111 0 Td 45.640432 Tz (interaction. ) Tj 3.519444 0 Td 52.662037 Tz (Post-hoc ) Tj 2.741667 0 Td 50.636574 Tz (analyses ) Tj 2.663889 0 Td 52.5 Tz (using ) Tj 1.769444 0 Td 55.092593 Tz (Tukey's ) Tj 2.566667 0 Td 78.858025 Tz (HSD ) Tj 51.428571 0 0 51.428571 0 2213.772379 Tm 25.297222 0 Td 60.493827 Tz (method ) Tj 2.566667 0 Td 54.282407 Tz (\(C=0.05\) ) Tj 2.975 0 Td 59.143519 Tz (were ) Tj 1.769444 0 Td 56.17284 Tz (performed ) Tj 3.383333 0 Td 45.37037 Tz (to ) Tj 0.933333 0 Td 46.585648 Tz (identify ) Tj 2.644444 0 Td 48.316498 Tz (differences ) Tj 40 0 0 40 0 2175 Tm 32.5 0 Td 74.404762 Tz (between ) Tj 3.375 0 Td 61.111111 Tz (factor ) Tj 2.4 0 Td 55.952381 Tz (levels. ) Tj 51.428571 0 0 51.428571 0 2115.413793 Tm 26.036111 0 Td 55.092593 Tz (Fold ) Tj 1.497222 0 Td 57.253086 Tz (change ) Tj 2.275 0 Td 43.75 Tz (in ) Tj 0.719444 0 Td 57.523148 Tz (gene ) Tj 1.555556 0 Td 51.262626 Tz (expressions ) Tj 3.577778 0 Td 61.574074 Tz (was ) Tj 1.302778 0 Td 49.259259 Tz (calculated ) Tj 3.15 0 Td 52.5 Tz (using ) Tj 1.75 0 Td 50.771605 Tz (the ) Tj 51.428571 0 0 51.428571 0 2067 Tm 25.297222 0 Td 46.666667 Tz (delta ) Tj 1.672222 0 Td 46.666667 Tz (delta ) Tj 1.672222 0 Td 59.953704 Tz (Ct ) Tj 0.952778 0 Td 57.253086 Tz (\(AACt\) ) Tj 2.333333 0 Td 55.092593 Tz (method, ) Tj 2.605556 0 Td 59.62963 Tz (where ) Tj 2.138889 0 Td 45.833333 Tz (B-actin ) Tj 2.197222 0 Td 52.391975 Tz (served ) Tj 2.138889 0 Td 50.231481 Tz (as ) Tj 0.855556 0 Td 50.771605 Tz (the ) Tj 51.428571 0 0 51.428571 0 2025.745973 Tm 25.277778 0 Td 55.902778 Tz (housekeeping ) Tj 4.277778 0 Td 56.712963 Tz (gene ) Tj 1.633333 0 Td 47.530864 Tz (for ) Tj 1.108333 0 Td 51.353276 Tz (normalization ) Tj 4.258333 0 Td 59.41358 Tz (and ) Tj 1.302778 0 Td 55.092593 Tz (time ) Tj 1.575 0 Td 51.851852 Tz (Zero ) Tj 1.516667 0 Td 75.347222 Tz (MSCs ) Tj 51.428571 0 0 51.428571 0 1983.785166 Tm 25.316667 0 Td 51.851852 Tz (served ) Tj 2.080556 0 Td 51.851852 Tz (as ) Tj 0.875 0 Td 49.691358 Tz (the ) Tj 1.127778 0 Td 50.05144 Tz (reference ) Tj 2.916667 0 Td 57.037037 Tz (group ) Tj 1.983333 0 Td 46.018519 Tz (\(fold ) Tj 1.652778 0 Td 56.712963 Tz (change ) Tj 2.275 0 Td 51.851852 Tz (equal ) Tj 1.808333 0 Td 45.37037 Tz (to ) Tj 0.816667 0 Td 44.907407 Tz (unity\). ) Tj 51.428571 0 0 51.428571 0 1938.749073 Tm 25.316667 0 Td 51.203704 Tz (Delta ) Tj 1.769444 0 Td 58.333333 Tz (Ct ) Tj 0.913889 0 Td 53.796296 Tz (\(ACt\) ) Tj 1.827778 0 Td 50.231481 Tz (values ) Tj 2.041667 0 Td 59.143519 Tz (were ) Tj 1.613889 0 Td 51.131687 Tz (subjected ) Tj 2.975 0 Td 45.37037 Tz (to ) Tj 0.777778 0 Td 40.951178 Tz (statistical ) Tj 2.916667 0 Td 55.092593 Tz (analyzed ) Tj 51.428571 0 0 51.428571 0 1894.538089 Tm 25.277778 0 Td 54.282407 Tz (with ) Tj 1.477778 0 Td 74.537037 Tz (SASR ) Tj 2.022222 0 Td 53.877315 Tz (Software ) Tj 2.761111 0 Td 64.00463 Tz (\(SAS ) Tj 1.730556 0 Td 41.805556 Tz (Institute; ) Tj 2.683333 0 Td 51.203704 Tz (Cary, ) Tj 1.711111 0 Td 53.148148 Tz (N.C.\) ) Tj 1.75 0 Td 51.851852 Tz (using ) Tj 1.711111 0 Td 46.450617 Tz (linear ) Tj 51.428571 0 0 51.428571 0 1851.529833 Tm 25.297222 0 Td 50.854701 Tz (mixed-effects ) Tj 4.161111 0 Td 57.253086 Tz (models ) Tj 2.294444 0 Td 52.777778 Tz (through ) Tj 2.430556 0 Td 49.691358 Tz (the ) Tj 1.069444 0 Td 77.777778 Tz (PROC ) Tj 2.061111 0 Td 78.425926 Tz (MIXED ) Tj 2.527778 0 Td 54.372428 Tz (procedure ) 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Tm 17.325 0 Td 83.522727 Tz (US ) Tj 1.240909 0 Td 58.333333 Tz (8,404,256 ) Tj 3.388636 0 Td 74.242424 Tz (B2 ) Tj 52.857143 0 0 52.857143 0 2968 Tm 14.264865 0 Td 74.099099 Tz (17 ) Tj 40 0 0 40 0 2913 Tm 15.65 0 Td 97.5 Tz (TABLE ) Tj 3.125 0 Td 68.560606 Tz (3-continued ) Tj 40 0 0 40 0 2841.224242 Tm 12.75 0 Td 61.309524 Tz (Reduced ) Tj 2.75 0 Td 55.208333 Tz (Data ) Tj 1.575 0 Td 57.291667 Tz (from ) Tj 1.525 0 Td 52.777778 Tz (the ) Tj 1.15 0 Td 46.474359 Tz (Proliferation ) Tj 3.875 0 Td 60.119048 Tz (Example ) Tj 38.571429 0 0 38.571429 0 2775.674419 Tm 22.4 0 Td 90.740741 Tz (DNA ) Tj 2.67037 0 Td 69.135802 Tz (Avg ) Tj 1.477778 0 Td 87.860082 Tz (DNA ) Tj 40 0 0 40 0 2747 Tm 21.775 0 Td 48.958333 Tz (\(ng\) ) Tj 3.275 0 Td 48.958333 Tz (\(ng\) ) Tj 30 0 0 30 0 2780 Tm 37.733333 0 Td 69.444444 Tz (St ) Tj 1.1 0 Td 85.185185 Tz (Dew ) Tj 37.5 0 0 37.5 0 2747 Tm 10.026667 0 Td 72.592593 Tz (Day ) Tj 3.733333 0 Td 57.777778 Tz (Condition ) Tj 5.093333 0 Td 62.777778 Tz (Specimen ) Tj 11.76 0 Td 52.222222 Tz (\(ng\) ) Tj 30 0 0 30 0 2685 Tm 29.4 0 Td 75.925926 Tz (186 ) Tj 30 0 0 30 0 2652 Tm 29.333333 0 Td 79.62963 Tz (227 ) Tj 30 0 0 30 0 2619 Tm 29.333333 0 Td 79.62963 Tz (288 ) Tj 30 0 0 30 0 2585 Tm 28.9 0 Td 79.166667 Tz (1274 ) Tj 30 0 0 30 0 2552 Tm 28.9 0 Td 77.777778 Tz (1636 ) Tj 30 0 0 30 0 2519 Tm 28.9 0 Td 79.166667 Tz (1504 ) Tj 30 0 0 30 0 2486 Tm 29.333333 0 Td 75.925926 Tz (541 ) Tj 30 0 0 30 0 2452 Tm 29.333333 0 Td 79.62963 Tz (396 ) Tj 30 0 0 30 0 2419 Tm 29.333333 0 Td 77.777778 Tz (365 ) Tj 30 0 0 30 0 2386 Tm 29.333333 0 Td 79.62963 Tz (519 ) Tj 30 0 0 30 0 2353 Tm 29.366667 0 Td 77.777778 Tz (68O ) Tj 30 0 0 30 0 2319 Tm 29.3 0 Td 81.481481 Tz (422 ) Tj 35 0 0 35 0 2684.639485 Tm 13.942857 0 Td 64.502165 Tz (Composition ) Tj 4.514286 0 Td 66.666667 Tz (2 ) Tj 10.428571 0 Td 69.84127 Tz (234 ) Tj 4.371429 0 Td 61.904762 Tz (51 ) Tj 34 0 0 34 0 2584.283186 Tm 10.294118 0 Td 66.176471 Tz (14 ) Tj 1.029412 0 Td 63.72549 Tz (days ) Tj 4.794118 0 Td 86.601307 Tz (TCP ) Tj 13.235294 0 Td 66.176471 Tz (1471 ) Tj 4.5 0 Td 65.359477 Tz (183 ) Tj 35 0 0 35 0 2485.742489 Tm 13.942857 0 Td 64.502165 Tz (Composition ) Tj 4.571429 0 Td 47.619048 Tz (1 ) Tj 10.342857 0 Td 71.428571 Tz (434 ) Tj 4.4 0 Td 69.047619 Tz (94 ) Tj 35 0 0 35 0 2385.659919 Tm 13.942857 0 Td 64.502165 Tz (Composition ) Tj 4.514286 0 Td 66.666667 Tz (2 ) Tj 10.428571 0 Td 68.253968 Tz (S4O ) Tj 4 0 Td 65.079365 Tz (130 ) Tj 51.428571 0 0 51.428571 0 2178.04 Tm 13.552778 0 Td 61.111111 Tz (Example ) Tj 2.741667 0 Td 58.333333 Tz (2 ) Tj 51.428571 0 0 51.428571 0 2085.534653 Tm 7.097222 0 Td 53.148148 Tz (Table ) Tj 1.788889 0 Td 35.648148 Tz (1 ) Tj 0.447222 0 Td 44.444444 Tz (details ) Tj 2.002778 0 Td 49.691358 Tz (the ) Tj 1.030556 0 Td 50.084175 Tz (biomaterial ) Tj 3.422222 0 Td 55.812757 Tz (composite ) Tj 3.130556 0 Td 51.58179 Tz (formulations ) Tj 3.830556 0 Td 61.574074 Tz (and ) Tj 51.428571 0 0 51.428571 0 2041.754839 Tm 6.358333 0 Td 50.084175 Tz (preparation ) Tj 3.597222 0 Td 59.953704 Tz (of ) Tj 0.913889 0 Td 49.691358 Tz (the ) Tj 1.205556 0 Td 51.041667 Tz (formulations ) Tj 4.005556 0 Td 63.734568 Tz (F1+ ) Tj 1.438889 0 Td 59.41358 Tz (and ) Tj 1.4 0 Td 61.574074 Tz (F2+ ) Tj 1.438889 0 Td 57.685185 Tz (which ) Tj 2.061111 0 Td 59.143519 Tz (were ) Tj 51.428571 0 0 51.428571 0 2001.703529 Tm 6.358333 0 Td 50 Tz (created ) Tj 2.236111 0 Td 53.240741 Tz (through ) Tj 2.372222 0 Td 51.388889 Tz (syringe ) Tj 2.313889 0 Td 51.851852 Tz (mixing. ) Tj 2.313889 0 Td 84.259259 Tz (A ) Tj 0.622222 0 Td 51.388889 Tz (Syringe ) Tj 2.294444 0 Td 50.87963 Tz (containing ) Tj 3.188889 0 Td 59.41358 Tz (sodium ) Tj 51.428571 0 0 51.428571 0 1955.420513 Tm 6.358333 0 Td 48.206019 Tz (alginate ) Tj 2.547222 0 Td 54.166667 Tz (\(Sigma; ) Tj 2.566667 0 Td 41.049383 Tz (St. ) Tj 1.05 0 Td 49.691358 Tz (Louis, ) Tj 2.061111 0 Td 58.333333 Tz (Mo.\) ) Tj 1.652778 0 Td 49.016204 Tz (Solution ) Tj 2.586111 0 Td 43.75 Tz (at ) Tj 0.758333 0 Td 53.148148 Tz (twice ) Tj 1.827778 0 Td 49.691358 Tz (the ) Tj 1.127778 0 Td 42.777778 Tz (final ) Tj 51.428571 0 0 51.428571 0 1911.742574 Tm 6.358333 0 Td 50.605413 Tz (concentration ) Tj 4.219444 0 Td 60.493827 Tz (was ) Tj 1.361111 0 Td 54.62963 Tz (coupled ) Tj 2.508333 0 Td 46.990741 Tz (to ) Tj 0.797222 0 Td 58.333333 Tz (a ) Tj 0.602778 0 Td 55.632716 Tz (second ) Tj 2.236111 0 Td 51.527778 Tz (containing ) Tj 3.383333 0 Td 56.481481 Tz (125-150 ) Tj 51.428571 0 0 51.428571 0 1868.607477 Tm 6.416667 0 Td 54.398148 Tz (umf-tricalcium ) Tj 4.725 0 Td 54.73251 Tz (phosphate ) Tj 3.072222 0 Td 58.796296 Tz (\(B-TCP\) ) Tj 2.566667 0 Td 51.446759 Tz (granules ) Tj 2.625 0 Td 55.092593 Tz (\(Plasma ) Tj 2.527778 0 Td 44.444444 Tz (Biotal, ) Tj 51.428571 0 0 51.428571 0 1826.14 Tm 6.377778 0 Td 44.074074 Tz (Ltd.: ) Tj 1.691667 0 Td 51.262626 Tz (Derbyshire, ) Tj 3.694444 0 Td 55.092593 Tz (United ) Tj 2.275 0 Td 58.333333 Tz (Kingdom\), ) Tj 3.441667 0 Td 53.703704 Tz (calcium ) Tj 2.586111 0 Td 46.296296 Tz (sulfate ) Tj 2.236111 0 Td 63.194444 Tz (dihy ) Tj 51.428571 0 0 51.428571 0 1781.168224 Tm 6.358333 0 Td 48.611111 Tz (drate ) Tj 1.594444 0 Td 69.675926 Tz (\(USG ) Tj 1.788889 0 Td 50.231481 Tz (Corp.; ) Tj 1.944444 0 Td 54.282407 Tz (Chicago, ) Tj 2.761111 0 Td 33.487654 Tz (Ill.\), ) Tj 1.322222 0 Td 66.111111 Tz (human ) Tj 2.080556 0 Td 51.851852 Tz (concentrated ) Tj 3.811111 0 Td 45.37037 Tz (platelet ) Tj 51.428571 0 0 51.428571 0 1735 Tm 6.358333 0 Td 47.170782 Tz (releasate ) Tj 2.955556 0 Td 56.944444 Tz (\(hCPR\), ) Tj 2.8 0 Td 51.851852 Tz (and, ) Tj 1.652778 0 Td 48.611111 Tz (for ) Tj 1.302778 0 Td 54.282407 Tz (F2+, ) Tj 1.730556 0 Td 58.333333 Tz (sodium ) Tj 2.508333 0 Td 43.518519 Tz (citrate ) Tj 2.275 0 Td 46.180556 Tz (\(Fisher; ) Tj 51.428571 0 0 51.428571 0 1695.043011 Tm 6.358333 0 Td 57.523148 Tz (Waltham, ) Tj 2.936111 0 Td 50.462963 Tz (Mass.\). ) Tj 2.275 0 Td 61.574074 Tz (Components ) Tj 3.869444 0 Td 59.143519 Tz (were ) Tj 1.594444 0 Td 58.333333 Tz (mixed ) Tj 1.925 0 Td 58.333333 Tz (by ) Tj 0.894444 0 Td 54.552469 Tz (twenty ) Tj 2.1 0 Td 51.851852 Tz (passes ) Tj 52.857143 0 0 52.857143 0 1648.514286 Tm 6.167568 0 Td 56.306306 Tz (between ) Tj 2.572973 0 Td 49.399399 Tz (the ) Tj 1.097297 0 Td 53.603604 Tz (coupled ) Tj 2.459459 0 Td 46.946947 Tz (syringes, ) Tj 2.743243 0 Td 57.387387 Tz (which ) Tj 1.948649 0 Td 46.509009 Tz (also ) Tj 1.381081 0 Td 42.742743 Tz (initiated ) Tj 2.516216 0 Td 53.603604 Tz (an ) Tj 0.889189 0 Td 46.666667 Tz (ionic ) Tj 51.428571 0 0 51.428571 0 1607.093943 Tm 6.358333 0 Td 52.314815 Tz (polymerization ) Tj 4.647222 0 Td 46.296296 Tz (setting ) Tj 2.197222 0 Td 45.37037 Tz (reaction. ) Tj 2.722222 0 Td 53.472222 Tz (This ) Tj 1.575 0 Td 53.240741 Tz (mixture ) Tj 2.45 0 Td 56.17284 Tz (can ) Tj 1.244444 0 Td 58.333333 Tz (be ) Tj 0.933333 0 Td 47.530864 Tz (easily ) Tj 51.428571 0 0 51.428571 0 1564.894737 Tm 6.358333 0 Td 49.691358 Tz (delivered ) Tj 3.091667 0 Td 54.166667 Tz (through ) Tj 2.741667 0 Td 46.450617 Tz (injection ) Tj 2.975 0 Td 48.611111 Tz (via ) Tj 1.302778 0 Td 55.092593 Tz (a ) Tj 0.816667 0 Td 55.092593 Tz (16-gauge ) Tj 3.072222 0 Td 57.685185 Tz (hypodermic ) Tj 51.428571 0 0 51.428571 0 1518.688504 Tm 6.358333 0 Td 52.932099 Tz (needle ) Tj 2.158333 0 Td 47.962963 Tz (prior ) Tj 1.633333 0 Td 48.611111 Tz (to ) Tj 0.836111 0 Td 48.148148 Tz (curing. ) Tj 2.275 0 Td 48.611111 Tz (Additionally, ) Tj 4.063889 0 Td 49.016204 Tz (material ) Tj 2.605556 0 Td 48.611111 Tz (controls ) Tj 2.605556 0 Td 54.282407 Tz (with ) Tj 51.428571 0 0 51.428571 0 1474.807018 Tm 6.358333 0 Td 52.572016 Tz (deionized ) Tj 2.955556 0 Td 55.092593 Tz (water ) Tj 1.769444 0 Td 49.691358 Tz (replacing ) Tj 2.8 0 Td 72.106481 Tz (hCPR ) Tj 1.886111 0 Td 59.143519 Tz (were ) Tj 1.536111 0 Td 50 Tz (created ) Tj 2.236111 0 Td 58.333333 Tz (and ) Tj 1.166667 0 Td 52.5 Tz (designated ) Tj 51.428571 0 0 51.428571 0 1429.338403 Tm 6.358333 0 Td 50.231481 Tz (as ) Tj 0.875 0 Td 62.654321 Tz (F1- ) Tj 1.322222 0 Td 60.493827 Tz (and ) Tj 1.341667 0 Td 53.472222 Tz (F2-. ) Tj 1.516667 0 Td 72.916667 Tz (hCPR ) Tj 1.983333 0 Td 61.574074 Tz (was ) Tj 1.322222 0 Td 53.472222 Tz (prepared ) Tj 2.8 0 Td 55.740741 Tz (under ) Tj 1.866667 0 Td 55.092593 Tz (an ) Tj 0.952778 0 Td 44.622507 Tz (Institutional ) Tj 51.428571 0 0 51.428571 0 1389.006017 Tm 6.377778 0 Td 59.953704 Tz (Review ) Tj 2.411111 0 Td 55.324074 Tz (Board-approved ) Tj 4.822222 0 Td 50.636574 Tz (protocol ) Tj 2.625 0 Td 45.37037 Tz (at ) Tj 0.719444 0 Td 48.611111 Tz (the ) Tj 1.069444 0 Td 50.555556 Tz (University ) Tj 3.227778 0 Td 58.333333 Tz (of ) Tj 0.797222 0 Td 98.302469 Tz (Mem ) Tj 51.428571 0 0 51.428571 0 1346.206675 Tm 6.358333 0 Td 50.231481 Tz (phis ) Tj 1.516667 0 Td 50.771605 Tz (\(approval ) Tj 3.091667 0 Td 63.734568 Tz (IRB ) Tj 1.497222 0 Td 45.833333 Tz (#/date: ) Tj 2.294444 0 Td 55.092593 Tz (H11-16/Sep. ) Tj 3.966667 0 Td 42.12963 Tz (9, ) Tj 0.836111 0 Td 57.037037 Tz (2010\) ) Tj 2.022222 0 Td 54.166667 Tz (through ) Tj 51.428571 0 0 51.428571 0 1301.247953 Tm 6.358333 0 Td 58.038721 Tz (methodology ) Tj 3.966667 0 Td 54.62963 Tz (adapted ) Tj 2.411111 0 Td 57.523148 Tz (from ) Tj 1.497222 0 Td 59.62963 Tz (White ) Tj 1.886111 0 Td 60.493827 Tz (and ) Tj 1.205556 0 Td 50.05144 Tz (Jennings. ) Tj 2.858333 0 Td 59.773663 Tz (McCanless ) Tj 3.344444 0 Td 51.851852 Tz (J ) Tj 51.428571 0 0 51.428571 0 1257.336996 Tm 6.377778 0 Td 56.712963 Tz (D, ) Tj 0.816667 0 Td 52.662037 Tz (Jennings ) Tj 2.702778 0 Td 34.027778 Tz (L. ) Tj 0.563889 0 Td 55.092593 Tz (K. ) Tj 0.777778 0 Td 59.143519 Tz (Cole ) Tj 1.536111 0 Td 59.41358 Tz (JA, ) Tj 1.244444 0 Td 60.277778 Tz (Bumgardner ) Tj 3.694444 0 Td 62.654321 Tz (JD, ) Tj 1.263889 0 Td 59.259259 Tz (Haggard ) Tj 2.625 0 Td 51.851852 Tz (W. ) Tj 0.777778 0 Td 56.712963 Tz (O. ) Tj 0.855556 0 Td 51.851852 Tz (In ) Tj 51.428571 0 0 51.428571 0 1214.359223 Tm 6.358333 0 Td 45.37037 Tz (vitro ) Tj 1.672222 0 Td 46.018519 Tz (differentiation ) Tj 4.433333 0 Td 59.41358 Tz (and ) Tj 1.361111 0 Td 49.826389 Tz (biocompatibility ) Tj 5.094444 0 Td 59.953704 Tz (of ) Tj 0.952778 0 Td 59.806397 Tz (mesenchymal ) Tj 51.428571 0 0 51.428571 0 1171.387952 Tm 6.377778 0 Td 55.902778 Tz (stem ) Tj 1.536111 0 Td 44.074074 Tz (cells ) Tj 1.516667 0 Td 59.953704 Tz (on ) Tj 0.913889 0 Td 55.092593 Tz (a ) Tj 0.544444 0 Td 51.851852 Tz (novel ) Tj 1.730556 0 Td 44.965278 Tz (platelet ) Tj 2.333333 0 Td 48.935185 Tz (releasate-containing ) Tj 6.047222 0 Td 47.314815 Tz (injectable ) Tj 51.428571 0 0 51.428571 0 1125.73174 Tm 6.358333 0 Td 52.824074 Tz (composite. ) Tj 3.558333 0 Td 48.611111 Tz (J ) Tj 0.7 0 Td 63.194444 Tz (Biomed ) Tj 2.683333 0 Td 58.333333 Tz (Mater ) Tj 2.119444 0 Td 59.41358 Tz (Res ) Tj 1.419444 0 Td 58.333333 Tz (A. ) Tj 1.108333 0 Td 54.444444 Tz (2012: ) Tj 2.119444 0 Td 52.121914 Tz (100\(1\):220-9 ) Tj 51.428571 0 0 51.428571 0 1089.078947 Tm 6.338889 0 Td 55.632716 Tz (hereby ) Tj 2.177778 0 Td 47.890947 Tz (specially ) Tj 2.8 0 Td 51.041667 Tz (incorporated ) Tj 3.830556 0 Td 59.953704 Tz (by ) Tj 0.933333 0 Td 47.314815 Tz (reference. ) Tj 51.428571 0 0 51.428571 0 1044.641232 Tm 7.116667 0 Td 52.175926 Tz (Processing ) Tj 3.5 0 Td 59.41358 Tz (was ) Tj 1.458333 0 Td 55.452675 Tz (conducted ) Tj 3.325 0 Td 45.37037 Tz (at ) Tj 0.913889 0 Td 62.384259 Tz (room ) Tj 1.866667 0 Td 52.73569 Tz (temperature ) Tj 3.830556 0 Td 50.231481 Tz (unless ) Tj 51.428571 0 0 51.428571 0 1003.390525 Tm 6.358333 0 Td 54.444444 Tz (noted ) Tj 1.866667 0 Td 44.020062 Tz (differently. ) Tj 3.519444 0 Td 60.763889 Tz (~120 ) Tj 1.691667 0 Td 59.953704 Tz (ml ) Tj 1.011111 0 Td 59.953704 Tz (of ) Tj 0.855556 0 Td 55.740741 Tz (blood ) Tj 1.925 0 Td 61.574074 Tz (was ) Tj 1.361111 0 Td 52.662037 Tz (acquired ) Tj 2.741667 0 Td 61.574074 Tz (by ) Tj 1.05 0 Td 59.953704 Tz (Veni ) Tj 51.428571 0 0 51.428571 0 960.514851 Tm 6.358333 0 Td 53.06713 Tz (puncture ) Tj 2.722222 0 Td 46.180556 Tz (into ) Tj 1.283333 0 Td 63.194444 Tz (30 ) Tj 0.894444 0 Td 51.851852 Tz (cc ) Tj 0.816667 0 Td 49.826389 Tz (syringes ) Tj 2.566667 0 Td 59.41358 Tz (and ) Tj 1.244444 0 Td 47.43266 Tz (transferred ) Tj 3.305556 0 Td 43.75 Tz (to ) Tj 0.719444 0 Td 61.574074 Tz (50 ) Tj 0.913889 0 Td 59.953704 Tz (ml ) Tj 0.894444 0 Td 50 Tz (conical ) Tj 51.428571 0 0 51.428571 0 920.711443 Tm 6.358333 0 Td 53.846154 Tz (polypropylene ) Tj 4.491667 0 Td 51.203704 Tz (tubes ) Tj 1.866667 0 Td 50.87963 Tz (containing ) Tj 3.363889 0 Td 43.055556 Tz (citrate ) Tj 2.1 0 Td 44.444444 Tz (buffer. ) Tj 2.216667 0 Td 57.253086 Tz (Plasma ) Tj 2.352778 0 Td 61.574074 Tz (and ) Tj 51.428571 0 0 51.428571 0 879.660955 Tm 6.338889 0 Td 51.851852 Tz (buffy ) Tj 1.866667 0 Td 49.421296 Tz (coat ) Tj 1.458333 0 Td 50.231481 Tz (Supernatants ) Tj 3.908333 0 Td 59.143519 Tz (were ) Tj 1.672222 0 Td 48.971193 Tz (collected ) Tj 2.877778 0 Td 46.018519 Tz (after ) Tj 1.652778 0 Td 48.611111 Tz (centrifugation ) Tj 51.428571 0 0 51.428571 0 836.309002 Tm 6.377778 0 Td 54.166667 Tz (\(600g\). ) Tj 2.547222 0 Td 44.650206 Tz (Platelets ) Tj 2.625 0 Td 59.143519 Tz (were ) Tj 1.613889 0 Td 51.851852 Tz (then ) Tj 1.458333 0 Td 51.851852 Tz (concentrated ) Tj 3.908333 0 Td 46.990741 Tz (in ) Tj 0.758333 0 Td 56.712963 Tz (plasma ) Tj 2.275 0 Td 54.62963 Tz (through ) Tj 51.428571 0 0 51.428571 0 795.35741 Tm 6.358333 0 Td 47.685185 Tz (further ) Tj 2.197222 0 Td 48.611111 Tz (centrifugation ) Tj 4.375 0 Td 52.391975 Tz (\(2,000 ) Tj 2.080556 0 Td 44.290123 Tz (g\), ) Tj 1.088889 0 Td 54.62963 Tz (removal ) Tj 2.547222 0 Td 59.953704 Tz (of ) Tj 0.875 0 Td 48.611111 Tz (the ) Tj 1.127778 0 Td 55.740741 Tz (upper ) Tj 1.886111 0 Td 53.472222 Tz (cell ) Tj 51.428571 0 0 51.428571 0 752.898409 Tm 6.358333 0 Td 47.800926 Tz (free ) Tj 1.341667 0 Td 56.712963 Tz (plasma ) Tj 2.255556 0 Td 65.625 Tz (\(80% ) Tj 1.827778 0 Td 58.873457 Tz (volume ) Tj 2.352778 0 Td 47.43266 Tz (reduction\), ) Tj 3.344444 0 Td 58.333333 Tz (and ) Tj 1.283333 0 Td 51.58179 Tz (resuspension ) Tj 3.947222 0 Td 46.180556 Tz (into ) Tj 51.428571 0 0 51.428571 0 713.474736 Tm 6.338889 0 Td 50.771605 Tz (the ) Tj 1.05 0 Td 54.372428 Tz (remaining ) Tj 3.052778 0 Td 53.240741 Tz (plasma. ) Tj 2.411111 0 Td 45.121083 Tz (Platelet-rich ) Tj 3.655556 0 Td 56.712963 Tz (plasma ) Tj 2.197222 0 Td 58.981481 Tz (\(PRP\) ) Tj 1.905556 0 Td 60.493827 Tz (was ) Tj 1.205556 0 Td 56.712963 Tz (pooled ) Tj 51.428571 0 0 51.428571 0 667 Tm 6.377778 0 Td 49.691358 Tz (\(n=5\), ) Tj 1.886111 0 Td 59.41358 Tz (and ) Tj 1.186111 0 Td 47.222222 Tz (clotted ) Tj 2.080556 0 Td 53.472222 Tz (with ) Tj 1.477778 0 Td 69.135802 Tz (10% ) Tj 1.380556 0 Td 49.691358 Tz (\(wt/v\) ) Tj 1.905556 0 Td 54.166667 Tz (calcium ) Tj 2.391667 0 Td 50.231481 Tz (chloride ) Tj 2.527778 0 Td 55.092593 Tz (\(50 ) Tj 1.108333 0 Td 49.907407 Tz (ul/ml ) Tj 51.428571 0 0 51.428571 0 627.226205 Tm 6.358333 0 Td 58.333333 Tz (of ) Tj 0.797222 0 Td 56.388889 Tz (PRP\). ) Tj 1.847222 0 Td 50.231481 Tz (Clotting ) Tj 2.566667 0 Td 53.06713 Tz (occurred ) Tj 2.761111 0 Td 46.450617 Tz (for ) Tj 0.972222 0 Td 61.574074 Tz (one ) Tj 1.244444 0 Td 56.712963 Tz (hour ) Tj 1.516667 0 Td 43.75 Tz (at ) Tj 0.7 0 Td 55.092593 Tz (37 ) Tj 1.186111 0 Td 55.092593 Tz (C. ) Tj 0.816667 0 Td 72.916667 Tz (hCPR ) Tj 1.905556 0 Td 63.734568 Tz (was ) Tj 51.428571 0 0 51.428571 0 585.226277 Tm 6.358333 0 Td 51.041667 Tz (expelled ) Tj 2.605556 0 Td 57.523148 Tz (from ) Tj 1.555556 0 Td 48.611111 Tz (the ) Tj 1.011111 0 Td 42.12963 Tz (clot, ) Tj 1.419444 0 Td 42.12963 Tz (sterile ) Tj 1.905556 0 Td 42.939815 Tz (filtered ) Tj 2.216667 0 Td 50.555556 Tz (\(0.20 ) Tj 1.691667 0 Td 52.662037 Tz (um\), ) Tj 1.438889 0 Td 58.333333 Tz (and ) Tj 1.186111 0 Td 49.691358 Tz (stored ) Tj 1.886111 0 Td 46.990741 Tz (at ) Tj 51.428571 0 0 51.428571 0 544.431274 Tm 6.377778 0 Td 57.523148 Tz (-80 ) Tj 1.613889 0 Td 53.472222 Tz (C. ) Tj 0.894444 0 Td 57.613169 Tz (Specimens ) Tj 3.383333 0 Td 46.450617 Tz (for ) Tj 1.05 0 Td 43.75 Tz (this ) Tj 1.263889 0 Td 63.194444 Tz (work ) Tj 1.711111 0 Td 58.333333 Tz (were ) Tj 1.613889 0 Td 50.925926 Tz (created ) Tj 2.352778 0 Td 52.5 Tz (using ) Tj 1.808333 0 Td 42.12963 Tz (sterile ) Tj 51.428571 0 0 51.428571 0 502.224199 Tm 6.358333 0 Td 46.990741 Tz (materials. ) Tj 2.994444 0 Td 52.256944 Tz (Alginate ) Tj 2.663889 0 Td 48.611111 Tz (solutions ) Tj 2.819444 0 Td 58.333333 Tz (were ) Tj 1.575 0 Td 49.789562 Tz (autoclaved. ) Tj 3.461111 0 Td 58.796296 Tz (Calcium ) Tj 2.663889 0 Td 42.777778 Tz (salts ) Tj 51.428571 0 0 51.428571 0 460.041032 Tm 6.338889 0 Td 59.953704 Tz (were ) Tj 1.536111 0 Td 71.944444 Tz (gamma ) Tj 2.313889 0 Td 44.191919 Tz (irradiated. ) Tj 3.072222 0 Td 62.114198 Tz (Sodium ) Tj 2.372222 0 Td 41.319444 Tz (citrate. ) Tj 2.138889 0 Td 64.166667 Tz (hCPR, ) Tj 2.041667 0 Td 59.41358 Tz (and ) Tj 1.186111 0 Td 52.932099 Tz (deionized ) Tj 51.428571 0 0 51.428571 0 418 Tm 6.338889 0 Td 55.092593 Tz (water ) Tj 1.788889 0 Td 46.180556 Tz (\(for ) Tj 1.263889 0 Td 62.654321 Tz (F1- ) Tj 1.283333 0 Td 58.333333 Tz (and ) Tj 1.225 0 Td 57.253086 Tz (F2-formulas\) ) Tj 4.277778 0 Td 58.333333 Tz (were ) Tj 1.536111 0 Td 42.592593 Tz (sterile ) Tj 1.944444 0 Td 41.049383 Tz (filtered; ) Tj 2.411111 0 Td 42.12963 Tz (sterile ) Tj 51.428571 0 0 51.428571 0 379.217631 Tm 6.377778 0 Td 58.333333 Tz (Sodium ) Tj 2.683333 0 Td 43.055556 Tz (citrate ) Tj 2.352778 0 Td 47.890947 Tz (Solutions ) Tj 3.15 0 Td 59.143519 Tz (were ) Tj 1.925 0 Td 49.789562 Tz (lyophilized ) Tj 3.830556 0 Td 43.75 Tz (in ) Tj 1.108333 0 Td 49.826389 Tz (Syringes ) Tj 51.428571 0 0 51.428571 0 337.435208 Tm 6.358333 0 Td 55.902778 Tz (equipped ) Tj 2.819444 0 Td 52.662037 Tz (with ) Tj 1.419444 0 Td 54.282407 Tz (0.20 ) Tj 1.477778 0 Td 72.916667 Tz (um ) Tj 1.011111 0 Td 51.388889 Tz (syringe ) Tj 2.313889 0 Td 38.425926 Tz (filters ) Tj 1.788889 0 Td 45.37037 Tz (to ) Tj 0.680556 0 Td 50.771605 Tz (obtain ) Tj 1.983333 0 Td 51.851852 Tz (a ) Tj 0.466667 0 Td 42.12963 Tz (sterile ) Tj 1.925 0 Td 43.209877 Tz (solid. ) Tj 51.428571 0 0 51.428571 0 294.565657 Tm 6.358333 0 Td 51.041667 Tz (Test ) Tj 1.438889 0 Td 55.812757 Tz (specimens ) Tj 3.227778 0 Td 59.143519 Tz (were ) Tj 1.613889 0 Td 50.925926 Tz (created ) Tj 2.352778 0 Td 53.472222 Tz (with ) Tj 1.516667 0 Td 59.953704 Tz (~3 ) Tj 1.011111 0 Td 59.953704 Tz (ml ) Tj 0.972222 0 Td 56.388889 Tz (mixes ) Tj 1.925 0 Td 58.981481 Tz (which ) Tj 2.002778 0 Td 59.143519 Tz (were ) Tj 51.428571 0 0 51.428571 0 253.320303 Tm 6.358333 0 Td 54.62963 Tz (allowed ) Tj 2.488889 0 Td 43.75 Tz (to ) Tj 0.777778 0 Td 51.041667 Tz (cure ) Tj 1.419444 0 Td 51.311728 Tz (within ) Tj 2.022222 0 Td 58.333333 Tz (3 ) Tj 0.583333 0 Td 53.472222 Tz (cc ) Tj 0.836111 0 Td 48.251029 Tz (syringes; ) Tj 2.8 0 Td 46.018519 Tz (after ) Tj 1.555556 0 Td 51.851852 Tz (curing ) Tj 2.061111 0 Td 56.712963 Tz (\(~10 ) Tj 1.594444 0 Td 73.45679 Tz (min ) Tj 51.428571 0 0 51.428571 0 214.326139 Tm 6.338889 0 Td 44.830247 Tz (utes\), ) Tj 1.75 0 Td 51.388889 Tz (Syringe ) Tj 2.313889 0 Td 46.450617 Tz (injection ) Tj 2.644444 0 Td 48.611111 Tz (ports ) Tj 1.613889 0 Td 59.143519 Tz (were ) Tj 1.555556 0 Td 49.691358 Tz (cut ) Tj 1.011111 0 Td 49.691358 Tz (off ) Tj 0.972222 0 Td 59.41358 Tz (and ) Tj 1.205556 0 Td 48.971193 Tz (cylinders ) Tj 2.8 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 169.969231 Tm 6.358333 0 Td 49.421296 Tz (material ) Tj 2.566667 0 Td 59.143519 Tz (were ) Tj 1.575 0 Td 51.851852 Tz (expelled ) Tj 2.644444 0 Td 59.41358 Tz (and ) Tj 1.244444 0 Td 49.691358 Tz (cut ) Tj 1.05 0 Td 48.611111 Tz (to ) Tj 0.777778 0 Td 42.777778 Tz (size. ) Tj 30 0 0 30 0 2755 Tm 41.833333 0 Td 72.222222 Tz (5 ) Tj 30 0 0 30 0 2547 Tm 41.666667 0 Td 75 Tz (10 ) Tj 30 0 0 30 0 2339 Tm 41.666667 0 Td 72.222222 Tz (15 ) Tj 30 0 0 30 0 1923 Tm 41.6 0 Td 77.777778 Tz (25 ) Tj 30 0 0 30 0 1716 Tm 41.6 0 Td 80.555556 Tz (30 ) Tj 30 0 0 30 0 1507 Tm 41.6 0 Td 77.777778 Tz (35 ) Tj 30 0 0 30 0 1299 Tm 41.566667 0 Td 83.333333 Tz (40 ) Tj 30 0 0 30 0 1091 Tm 41.566667 0 Td 80.555556 Tz (45 ) Tj 30 0 0 30 0 883 Tm 41.6 0 Td 80.555556 Tz (50 ) Tj 30 0 0 30 0 675 Tm 41.6 0 Td 77.777778 Tz (55 ) Tj 30 0 0 30 0 467 Tm 41.633333 0 Td 77.777778 Tz (60 ) Tj 30 0 0 30 0 259 Tm 41.633333 0 Td 75 Tz (65 ) Tj 52.857143 0 0 52.857143 0 2968 Tm 32.672973 0 Td 74.099099 Tz (18 ) Tj 51.428571 0 0 51.428571 0 2913 Tm 26.016667 0 Td 49.21875 Tz (Platelet-Derived ) Tj 4.958333 0 Td 61.033951 Tz (Growth ) Tj 2.45 0 Td 51.311728 Tz (Factor ) Tj 2.061111 0 Td 64.814815 Tz (\(-BB ) Tj 1.788889 0 Td 58.333333 Tz (and ) Tj 1.283333 0 Td 68.055556 Tz (-AB\) ) Tj 1.866667 0 Td 64.814815 Tz (\(PDGF\) ) Tj 51.428571 0 0 51.428571 0 2873.116505 Tm 25.316667 0 Td 47.395833 Tz (Elution: ) Tj 2.488889 0 Td 61.574074 Tz (Growth ) Tj 2.45 0 Td 46.990741 Tz (factor ) Tj 1.886111 0 Td 47.222222 Tz (release ) Tj 2.197222 0 Td 60.493827 Tz (was ) Tj 1.283333 0 Td 51.851852 Tz (assessed ) Tj 2.683333 0 Td 53.240741 Tz (through ) Tj 2.430556 0 Td 48.611111 Tz (elution ) Tj 51.428571 0 0 51.428571 0 2832.065116 Tm 25.316667 0 Td 47.685185 Tz (studies ) Tj 2.138889 0 Td 52.662037 Tz (with ) Tj 1.438889 0 Td 76.967593 Tz (PDGF ) Tj 1.983333 0 Td 56.712963 Tz (sandwich ) Tj 2.858333 0 Td 55.34188 Tz (enzyme-linked ) Tj 4.472222 0 Td 58.582621 Tz (immunosorbent ) Tj 51.428571 0 0 51.428571 0 2791.583535 Tm 25.297222 0 Td 51.311728 Tz (assays ) Tj 2.022222 0 Td 59.143519 Tz (\(ELISAs\) ) Tj 2.994444 0 Td 53.324916 Tz (\(PeproTech: ) Tj 3.772222 0 Td 60.925926 Tz (Rocky ) Tj 2.041667 0 Td 42.12963 Tz (Hill, ) Tj 1.438889 0 Td 43.75 Tz (N.J.\). ) Tj 1.808333 0 Td 49.537037 Tz (Elution ) Tj 2.275 0 Td 61.574074 Tz (was ) Tj 51.428571 0 0 51.428571 0 2748.587708 Tm 25.297222 0 Td 55.452675 Tz (conducted ) Tj 3.208333 0 Td 57.523148 Tz (from ) Tj 1.652778 0 Td 47.530864 Tz (1.5 ) Tj 1.088889 0 Td 61.574074 Tz (ml ) Tj 0.952778 0 Td 55.812757 Tz (specimens ) Tj 3.247222 0 Td 46.180556 Tz (into ) Tj 1.322222 0 Td 46.450617 Tz (0.1 ) Tj 1.108333 0 Td 61.574074 Tz (wt ) Tj 0.933333 0 Td 97.222222 Tz (% ) Tj 0.816667 0 Td 56.018519 Tz (albumin ) Tj 2.566667 0 Td 48.611111 Tz (in ) Tj 51.428571 0 0 51.428571 0 2706.45541 Tm 25.277778 0 Td 55.092593 Tz (phosphate ) Tj 3.072222 0 Td 51.041667 Tz (buffered ) Tj 2.586111 0 Td 46.450617 Tz (saline ) Tj 1.788889 0 Td 59.62963 Tz (\(PBS\) ) Tj 1.866667 0 Td 50.231481 Tz (eluent ) Tj 1.905556 0 Td 45.37037 Tz (at ) Tj 0.641667 0 Td 59.62963 Tz (37C. ) Tj 1.925 0 Td 49.151235 Tz (\(n=4\). ) Tj 1.983333 0 Td 45.37037 Tz (1.5 ) Tj 0.972222 0 Td 61.574074 Tz (ml ) Tj 51.428571 0 0 51.428571 0 2666.212987 Tm 25.316667 0 Td 54.62963 Tz (samples ) Tj 2.488889 0 Td 59.953704 Tz (were ) Tj 1.575 0 Td 51.311728 Tz (pulled ) Tj 2.022222 0 Td 57.523148 Tz (from ) Tj 1.575 0 Td 56.712963 Tz (an ) Tj 0.875 0 Td 39.814815 Tz (initial ) Tj 1.866667 0 Td 61.574074 Tz (40 ) Tj 0.913889 0 Td 63.194444 Tz (ml ) Tj 0.933333 0 Td 43.75 Tz (at ) Tj 0.7 0 Td 58.333333 Tz (5 ) Tj 0.544444 0 Td 49.151235 Tz (hours, ) Tj 2.041667 0 Td 38.888889 Tz (1, ) Tj 0.661111 0 Td 42.12963 Tz (2, ) Tj 0.680556 0 Td 45.37037 Tz (3, ) Tj 51.428571 0 0 51.428571 0 2626.074349 Tm 25.297222 0 Td 58.333333 Tz (and ) Tj 1.225 0 Td 58.333333 Tz (5 ) Tj 0.563889 0 Td 49.259259 Tz (days. ) Tj 1.633333 0 Td 51.041667 Tz (Additionally ) Tj 3.888889 0 Td 43.75 Tz (to ) Tj 0.738889 0 Td 55.092593 Tz (monitor ) Tj 2.488889 0 Td 76.967593 Tz (PDGF ) Tj 2.041667 0 Td 47.314815 Tz (depletion, ) Tj 3.052778 0 Td 61.574074 Tz (400 ) Tj 1.322222 0 Td 42.12963 Tz (ul ) Tj 51.428571 0 0 51.428571 0 2582.233046 Tm 25.297222 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 72.916667 Tz (hCPR ) Tj 1.886111 0 Td 60.493827 Tz (was ) Tj 1.244444 0 Td 58.333333 Tz (added ) Tj 1.905556 0 Td 45.37037 Tz (to ) Tj 0.7 0 Td 59.953704 Tz (40 ) Tj 0.894444 0 Td 61.574074 Tz (ml ) Tj 0.875 0 Td 49.691358 Tz (eluent ) Tj 1.925 0 Td 58.333333 Tz (and ) Tj 1.186111 0 Td 57.87037 Tz (sampled ) Tj 2.547222 0 Td 46.990741 Tz (at ) Tj 0.719444 0 Td 53.472222 Tz (time ) Tj 1.419444 0 Td 46.666667 Tz (Zero, ) Tj 51.428571 0 0 51.428571 0 2539.68 Tm 25.316667 0 Td 55.092593 Tz (5 ) Tj 0.486111 0 Td 49.691358 Tz (hours, ) Tj 2.002778 0 Td 40.509259 Tz (1, ) Tj 0.641667 0 Td 43.75 Tz (2, ) Tj 0.680556 0 Td 43.75 Tz (3, ) Tj 0.7 0 Td 58.333333 Tz (and ) Tj 1.205556 0 Td 55.092593 Tz (5 ) Tj 0.505556 0 Td 55.092593 Tz (days ) Tj 1.477778 0 Td 49.151235 Tz (\(n=3\). ) Tj 1.983333 0 Td 74.537037 Tz (PDGF ) Tj 1.963889 0 Td 50.231481 Tz (concentrations ) Tj 4.394444 0 Td 59.143519 Tz (were ) Tj 51.428571 0 0 51.428571 0 2499.621762 Tm 25.277778 0 Td 52.5 Tz (taken ) Tj 1.944444 0 Td 50.231481 Tz (as ) Tj 0.933333 0 Td 50.771605 Tz (the ) Tj 1.244444 0 Td 54.166667 Tz (average ) Tj 2.625 0 Td 56.712963 Tz (of ) Tj 0.972222 0 Td 48.971193 Tz (duplicate ) Tj 3.033333 0 Td 68.055556 Tz (ELISA ) Tj 2.430556 0 Td 54.843305 Tz (measurements. ) Tj 51.428571 0 0 51.428571 0 2458.605988 Tm 25.297222 0 Td 48.971193 Tz (Material, ) Tj 2.8 0 Td 57.118056 Tz (specimen ) Tj 2.916667 0 Td 56.018519 Tz (number, ) Tj 2.508333 0 Td 58.333333 Tz (and ) Tj 1.186111 0 Td 55.092593 Tz (time ) Tj 1.477778 0 Td 58.333333 Tz (were ) Tj 1.555556 0 Td 51.851852 Tz (taken ) Tj 1.691667 0 Td 51.851852 Tz (as ) Tj 0.777778 0 Td 60.648148 Tz (indepen ) Tj 51.428571 0 0 51.428571 0 2417.889724 Tm 25.297222 0 Td 52.662037 Tz (dent ) Tj 1.438889 0 Td 52.391975 Tz (experimental ) Tj 4.005556 0 Td 45.37037 Tz (variables. ) Tj 51.428571 0 0 51.428571 0 2374.161644 Tm 26.036111 0 Td 57.253086 Tz (Simple ) Tj 2.352778 0 Td 55.416667 Tz (chemotaxis ) Tj 3.694444 0 Td 60.493827 Tz (was ) Tj 1.438889 0 Td 51.446759 Tz (assessed ) Tj 2.819444 0 Td 51.851852 Tz (with ) Tj 1.613889 0 Td 48.611111 Tz (the ) Tj 1.263889 0 Td 85.339506 Tz (RAW ) Tj 1.925 0 Td 53.148148 Tz (264.7 ) Tj 51.428571 0 0 51.428571 0 2334.166473 Tm 25.297222 0 Td 52.5 Tz (clone ) Tj 1.672222 0 Td 58.333333 Tz (TIB-71 ) Tj 2.313889 0 Td 62.654321 Tz (\(ATCC; ) Tj 2.430556 0 Td 55.092593 Tz (Manassas, ) Tj 3.130556 0 Td 49.421296 Tz (Va.\) ) Tj 1.322222 0 Td 57.992203 Tz (monocyte/macrophage ) Tj 51.428571 0 0 51.428571 0 2295.090909 Tm 25.297222 0 Td 43.75 Tz (cell ) Tj 1.186111 0 Td 46.180556 Tz (line ) Tj 1.244444 0 Td 54.79798 Tz (\(monocytes\) ) Tj 3.772222 0 Td 52.5 Tz (using ) Tj 1.691667 0 Td 52.777778 Tz (24-well ) Tj 2.333333 0 Td 48.971193 Tz (Transwell\(R\)plates ) Tj 5.463889 0 Td 52.662037 Tz (with ) Tj 1.380556 0 Td 58.333333 Tz (3 ) Tj 51.428571 0 0 51.428571 0 2252.321019 Tm 25.355556 0 Td 71.296296 Tz (um ) Tj 1.186111 0 Td 55.092593 Tz (pore ) Tj 1.633333 0 Td 53.098291 Tz (polycarbonate ) Tj 4.491667 0 Td 44.444444 Tz (inserts ) Tj 2.236111 0 Td 51.49177 Tz (\(Corning; ) Tj 3.169444 0 Td 52.314815 Tz (Lowell, ) Tj 2.566667 0 Td 50 Tz (Mass.\). ) Tj 51.428571 0 0 51.428571 0 2207.07056 Tm 25.316667 0 Td 62.87037 Tz (Lower ) Tj 2.061111 0 Td 50.555556 Tz (wells ) Tj 1.730556 0 Td 58.333333 Tz (were ) Tj 1.594444 0 Td 53.472222 Tz (loaded ) Tj 2.119444 0 Td 53.472222 Tz (with ) Tj 1.516667 0 Td 54.444444 Tz (Dulbecco's ) Tj 3.5 0 Td 53.472222 Tz (modified ) Tj 2.780556 0 Td 50.925926 Tz (Eagle's ) Tj 57.142857 0 0 57.142857 0 2169.669109 Tm 22.7675 0 Td 58.819444 Tz (medium ) Tj 2.345 0 Td 62.5 Tz (\(DMEM\), ) Tj 2.87 0 Td 53.958333 Tz (10 ) Tj 1.3475 0 Td 96.25 Tz (M ) Tj 0.7875 0 Td 48.074713 Tz (n-formyl-methionyl-leucyl-phe ) Tj 51.428571 0 0 51.428571 0 2124.48503 Tm 25.316667 0 Td 50.231481 Tz (nylalanine ) Tj 3.15 0 Td 61.033951 Tz (\(fMLP\) ) Tj 2.333333 0 Td 48.611111 Tz (in ) Tj 0.7 0 Td 80.37037 Tz (DMEM, ) Tj 2.508333 0 Td 55.092593 Tz (or ) Tj 0.777778 0 Td 58.333333 Tz (48 ) Tj 0.816667 0 Td 57.523148 Tz (hour ) Tj 1.477778 0 Td 47.685185 Tz (eluates ) Tj 2.138889 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 55.092593 Tz (F1+, ) Tj 1.477778 0 Td 54.282407 Tz (F2+, ) Tj 51.428571 0 0 51.428571 0 2083.431907 Tm 25.316667 0 Td 54.282407 Tz (F1-, ) Tj 1.536111 0 Td 60.493827 Tz (and ) Tj 1.341667 0 Td 53.472222 Tz (F2-. ) Tj 1.613889 0 Td 49.537037 Tz (Eluates ) Tj 2.333333 0 Td 58.333333 Tz (were ) Tj 1.633333 0 Td 55.902778 Tz (produced ) Tj 2.955556 0 Td 52.662037 Tz (with ) Tj 1.575 0 Td 45.37037 Tz (1.5 ) Tj 1.108333 0 Td 59.953704 Tz (ml ) Tj 0.991667 0 Td 49.016204 Tz (material ) Tj 51.428571 0 0 51.428571 0 2041.480048 Tm 25.316667 0 Td 55.092593 Tz (specimens ) Tj 3.208333 0 Td 46.990741 Tz (in ) Tj 0.738889 0 Td 58.333333 Tz (5 ) Tj 0.544444 0 Td 63.194444 Tz (ml ) Tj 0.952778 0 Td 59.953704 Tz (of ) Tj 0.797222 0 Td 81.018519 Tz (DMEM. ) Tj 2.605556 0 Td 76.157407 Tz (CELL ) Tj 1.983333 0 Td 81.481481 Tz (TRACKER ) Tj 3.577778 0 Td 84.259259 Tz (ORANGE ) Tj 51.428571 0 0 51.428571 0 2000.551515 Tm 25.316667 0 Td 52.777778 Tz (\(Lonza; ) Tj 2.45 0 Td 48.070988 Tz (Basel, ) Tj 1.925 0 Td 51.851852 Tz (Switzerland\) ) Tj 3.908333 0 Td 49.537037 Tz (labeled ) Tj 2.275 0 Td 57.613169 Tz (monocytes ) Tj 3.305556 0 Td 45.37037 Tz (\(3 ) Tj 0.777778 0 Td 68.055556 Tz (uM\) ) Tj 1.419444 0 Td 58.333333 Tz (were ) Tj 58.571429 0 0 58.571429 0 1958.018421 Tm 22.212195 0 Td 47.425474 Tz (loaded ) Tj 1.997561 0 Td 39.837398 Tz (into ) Tj 1.263415 0 Td 48.943089 Tz (upper ) Tj 1.707317 0 Td 45.528455 Tz (wells ) Tj 1.621951 0 Td 42.682927 Tz (in ) Tj 0.768293 0 Td 83.231707 Tz (DMEM ) Tj 2.270732 0 Td 49.322493 Tz (\(10 ) Tj 1.34878 0 Td 37.398374 Tz (cells/insert\), ) Tj 3.431707 0 Td 52.168022 Tz (and ) Tj 51.428571 0 0 51.428571 0 1916.390887 Tm 25.297222 0 Td 52.211934 Tz (migration ) Tj 2.955556 0 Td 61.574074 Tz (was ) Tj 1.244444 0 Td 55.092593 Tz (allowed ) Tj 2.411111 0 Td 46.990741 Tz (to ) Tj 0.719444 0 Td 55.092593 Tz (occur ) Tj 1.75 0 Td 48.611111 Tz (for ) Tj 0.972222 0 Td 58.333333 Tz (4 ) Tj 0.486111 0 Td 53.796296 Tz (hours ) Tj 1.769444 0 Td 49.151235 Tz (\(n=2\). ) Tj 1.886111 0 Td 60.277778 Tz (Those ) Tj 1.925 0 Td 44.722222 Tz (cells ) Tj 51.428571 0 0 51.428571 0 1877.420147 Tm 25.277778 0 Td 59.62963 Tz (which ) Tj 1.983333 0 Td 50.771605 Tz (did ) Tj 1.108333 0 Td 50.771605 Tz (not ) Tj 1.088889 0 Td 52.314815 Tz (migrate ) Tj 2.372222 0 Td 58.333333 Tz (were ) Tj 1.594444 0 Td 59.259259 Tz (removed ) Tj 2.683333 0 Td 51.851852 Tz (using ) Tj 1.711111 0 Td 51.851852 Tz (a ) Tj 0.525 0 Td 69.135802 Tz (PBS ) Tj 1.419444 0 Td 62.789352 Tz (dampened ) Tj 51.428571 0 0 51.428571 0 1833.275362 Tm 25.297222 0 Td 50.231481 Tz (cotton ) Tj 1.963889 0 Td 55.740741 Tz (Swab, ) Tj 1.827778 0 Td 58.333333 Tz (and ) Tj 1.186111 0 Td 51.851852 Tz (those ) Tj 1.711111 0 Td 58.333333 Tz (which ) Tj 1.905556 0 Td 59.41358 Tz (had ) Tj 1.244444 0 Td 53.472222 Tz (migrated ) Tj 2.722222 0 Td 59.143519 Tz (were ) Tj 1.555556 0 Td 48.971193 Tz (collected ) Tj 2.8 0 Td 46.990741 Tz (in ) Tj 51.428571 0 0 51.428571 0 1794.45098 Tm 25.277778 0 Td 50.771605 Tz (the ) Tj 1.088889 0 Td 55.740741 Tz (lower ) Tj 1.827778 0 Td 51.041667 Tz (well ) Tj 1.438889 0 Td 53.240741 Tz (through ) Tj 2.391667 0 Td 48.611111 Tz (centrifugation ) Tj 4.277778 0 Td 56.712963 Tz (\(400 ) Tj 1.497222 0 Td 43.75 Tz (g, ) Tj 0.797222 0 Td 53.472222 Tz (15 ) Tj 0.855556 0 Td 52.256944 Tz (minutes\) ) Tj 2.702778 0 Td 58.333333 Tz (of ) Tj 51.428571 0 0 51.428571 0 1749.707022 Tm 25.277778 0 Td 50.771605 Tz (the ) Tj 1.030556 0 Td 47.314815 Tz (plate ) Tj 1.575 0 Td 52.662037 Tz (with ) Tj 1.438889 0 Td 44.907407 Tz (inserts ) Tj 2.041667 0 Td 43.75 Tz (installed. ) Tj 2.761111 0 Td 50.555556 Tz (Cells ) Tj 1.672222 0 Td 45.37037 Tz (in ) Tj 0.7 0 Td 55.740741 Tz (lower ) Tj 1.788889 0 Td 50.555556 Tz (wells ) Tj 1.672222 0 Td 59.143519 Tz (were ) Tj 1.555556 0 Td 53.472222 Tz (then ) Tj 51.428571 0 0 51.428571 0 1713.844252 Tm 25.316667 0 Td 55.812757 Tz (Suspended ) Tj 3.305556 0 Td 48.611111 Tz (via ) Tj 1.186111 0 Td 50.771605 Tz (micropipette ) Tj 3.947222 0 Td 56.712963 Tz (mixing ) Tj 2.294444 0 Td 59.41358 Tz (and ) Tj 1.361111 0 Td 54.62963 Tz (counted ) Tj 2.566667 0 Td 46.342593 Tz (indirectly ) Tj 51.428571 0 0 51.428571 0 1668.848329 Tm 25.277778 0 Td 53.148148 Tz (using ) Tj 1.963889 0 Td 50.231481 Tz (fluorescence ) Tj 3.947222 0 Td 52.662037 Tz (spectroscopy ) Tj 4.141667 0 Td 58.333333 Tz (and ) Tj 1.380556 0 Td 58.333333 Tz (a ) Tj 0.7 0 Td 50.636574 Tz (standard ) Tj 2.8 0 Td 53.796296 Tz (curve ) Tj 1.963889 0 Td 56.712963 Tz (of ) Tj 52.857143 0 0 52.857143 0 1625.636927 Tm 24.613514 0 Td 56.406406 Tz (monocytes ) Tj 3.235135 0 Td 57.387387 Tz (which ) Tj 1.910811 0 Td 57.807808 Tz (had ) Tj 1.22973 0 Td 55.18018 Tz (been ) Tj 1.532432 0 Td 53.078078 Tz (seeded ) Tj 2.1 0 Td 45.720721 Tz (at ) Tj 0.681081 0 Td 49.399399 Tz (the ) Tj 1.078378 0 Td 40.990991 Tz (start ) Tj 1.4 0 Td 56.756757 Tz (of ) Tj 0.756757 0 Td 49.399399 Tz (the ) Tj 1.078378 0 Td 55.18018 Tz (experi ) Tj 51.428571 0 0 51.428571 0 1585.053942 Tm 25.297222 0 Td 53.796296 Tz (ment. ) Tj 2.061111 0 Td 52.932099 Tz (Values ) Tj 2.352778 0 Td 59.143519 Tz (were ) Tj 1.827778 0 Td 52.5 Tz (taken ) Tj 2.002778 0 Td 50.231481 Tz (as ) Tj 1.05 0 Td 49.691358 Tz (the ) Tj 1.322222 0 Td 53.703704 Tz (average ) Tj 2.683333 0 Td 56.712963 Tz (of ) Tj 1.030556 0 Td 47.138047 Tz (duplicates. ) Tj 51.428571 0 0 51.428571 0 1542.723606 Tm 25.297222 0 Td 50.434028 Tz (Transendothelial ) Tj 5.036111 0 Td 51.49177 Tz (migration ) Tj 2.955556 0 Td 60.493827 Tz (was ) Tj 1.244444 0 Td 51.446759 Tz (assessed ) Tj 2.625 0 Td 51.851852 Tz (using ) Tj 1.672222 0 Td 58.333333 Tz (a ) Tj 0.505556 0 Td 51.851852 Tz (very ) Tj 1.4 0 Td 49.074074 Tz (similar ) Tj 51.428571 0 0 51.428571 0 1499 Tm 25.297222 0 Td 57.87037 Tz (methods ) Tj 2.761111 0 Td 51.851852 Tz (as ) Tj 0.933333 0 Td 51.851852 Tz (described ) Tj 3.111111 0 Td 57.685185 Tz (above ) Tj 2.061111 0 Td 52.662037 Tz (with ) Tj 1.594444 0 Td 58.333333 Tz (8 ) Tj 0.719444 0 Td 71.296296 Tz (um ) Tj 1.225 0 Td 44.444444 Tz (inserts ) Tj 2.216667 0 Td 49.151235 Tz (\(n=2\); ) Tj 2.1 0 Td 41.049383 Tz (all ) Tj 51.428571 0 0 51.428571 0 1458.57529 Tm 25.297222 0 Td 48.611111 Tz (differences ) Tj 3.519444 0 Td 49.691358 Tz (are ) Tj 1.205556 0 Td 53.796296 Tz (noted ) Tj 1.905556 0 Td 46.990741 Tz (in ) Tj 0.894444 0 Td 48.611111 Tz (the ) Tj 1.166667 0 Td 49.583333 Tz (following. ) Tj 3.325 0 Td 62.87037 Tz (Lower ) Tj 2.158333 0 Td 49.826389 Tz (surfaces ) Tj 2.683333 0 Td 58.333333 Tz (of ) Tj 51.428571 0 0 51.428571 0 1417.591411 Tm 25.297222 0 Td 44.907407 Tz (inserts ) Tj 2.022222 0 Td 60.493827 Tz (had ) Tj 1.205556 0 Td 55.902778 Tz (been ) Tj 1.477778 0 Td 53.472222 Tz (coated ) Tj 2.061111 0 Td 53.472222 Tz (with ) Tj 1.4 0 Td 55.632716 Tz (bovine ) Tj 2.1 0 Td 51.446759 Tz (collagen ) Tj 2.625 0 Td 52.662037 Tz (type ) Tj 1.438889 0 Td 32.407407 Tz (I ) Tj 0.330556 0 Td 55.092593 Tz (\(50 ) Tj 1.4 0 Td 58.333333 Tz (g/ml ) Tj 51.428571 0 0 51.428571 0 1374.914894 Tm 25.297222 0 Td 49.691358 Tz (for ) Tj 1.147222 0 Td 32.407407 Tz (1 ) Tj 0.505556 0 Td 48.611111 Tz (hour\), ) Tj 2.002778 0 Td 58.333333 Tz (and ) Tj 1.302778 0 Td 55.740741 Tz (upper ) Tj 1.886111 0 Td 49.537037 Tz (surface ) Tj 2.294444 0 Td 62.654321 Tz (was ) Tj 1.361111 0 Td 52.932099 Tz (coated ) Tj 2.119444 0 Td 53.472222 Tz (with ) Tj 1.555556 0 Td 59.953704 Tz (50 ) Tj 0.972222 0 Td 43.75 Tz (ul ) Tj 0.758333 0 Td 58.333333 Tz (of ) Tj 0.836111 0 Td 59.953704 Tz (50 ) Tj 51.428571 0 0 51.428571 0 1333.800948 Tm 25.355556 0 Td 54.444444 Tz (ug/ml ) Tj 1.788889 0 Td 57.253086 Tz (growth ) Tj 2.255556 0 Td 50 Tz (factor-reduced ) Tj 4.394444 0 Td 76.5625 Tz (MATRIGEL ) Tj 3.869444 0 Td 69.135802 Tz (\(BD ) Tj 1.477778 0 Td 49.96142 Tz (Biosciences: ) Tj 51.428571 0 0 51.428571 0 1292.508197 Tm 25.316667 0 Td 50.636574 Tz (Franklin ) Tj 2.586111 0 Td 52.391975 Tz (Lakes, ) Tj 2.022222 0 Td 43.209877 Tz (N.J.\). ) Tj 1.652778 0 Td 87.5 Tz (A ) Tj 0.622222 0 Td 50.771605 Tz (confluent ) Tj 2.858333 0 Td 48.905724 Tz (endothelial ) Tj 3.363889 0 Td 57.973251 Tz (monolayer ) Tj 3.227778 0 Td 61.574074 Tz (was ) Tj 51.428571 0 0 51.428571 0 1252.30967 Tm 25.297222 0 Td 49.200337 Tz (established ) Tj 3.441667 0 Td 58.333333 Tz (on ) Tj 0.913889 0 Td 56.388889 Tz (upper ) Tj 1.866667 0 Td 44.830247 Tz (insert ) Tj 1.808333 0 Td 49.421296 Tz (Surfaces ) Tj 2.586111 0 Td 52.5 Tz (using ) Tj 1.769444 0 Td 46.018519 Tz (third ) Tj 1.575 0 Td 53.703704 Tz (passage ) Tj 2.45 0 Td 58.333333 Tz (pri ) Tj 51.428571 0 0 51.428571 0 1208.777494 Tm 25.297222 0 Td 61.574074 Tz (mary ) Tj 1.827778 0 Td 66.111111 Tz (human ) Tj 2.372222 0 Td 50.771605 Tz (umbilical ) Tj 3.15 0 Td 49.421296 Tz (vein ) Tj 1.555556 0 Td 48.905724 Tz (endothelial ) Tj 3.597222 0 Td 44.074074 Tz (cells ) Tj 1.691667 0 Td 68.055556 Tz (\(HUVECs\) ) Tj 58.571429 0 0 58.571429 0 1166 Tm 22.229268 0 Td 55.01355 Tz (\(ATCC, ) Tj 2.202439 0 Td 48.690154 Tz (Manassas, ) Tj 2.834146 0 Td 39.837398 Tz (Va.\). ) Tj 1.485366 0 Td 51.219512 Tz (10 ) Tj 1.07561 0 Td 67.344173 Tz (HUVECs ) Tj 2.629268 0 Td 53.353659 Tz (were ) Tj 1.485366 0 Td 47.899729 Tz (seeded ) Tj 1.963415 0 Td 54.065041 Tz (on ) Tj 0.853659 0 Td 43.631436 Tz (the ) Tj 51.428571 0 0 51.428571 0 1126.690446 Tm 25.277778 0 Td 57.685185 Tz (upper ) Tj 1.925 0 Td 44.830247 Tz (insert ) Tj 1.847222 0 Td 49.537037 Tz (Surface ) Tj 2.294444 0 Td 58.333333 Tz (and ) Tj 1.263889 0 Td 55.092593 Tz (allowed ) Tj 2.508333 0 Td 46.990741 Tz (to ) Tj 0.816667 0 Td 58.333333 Tz (expand ) Tj 2.333333 0 Td 47.530864 Tz (for ) Tj 1.05 0 Td 45.37037 Tz (five ) Tj 1.322222 0 Td 54.282407 Tz (days ) Tj 1.575 0 Td 46.990741 Tz (in ) Tj 51.428571 0 0 51.428571 0 1084.130552 Tm 25.277778 0 Td 55.632716 Tz (bovine ) Tj 2.430556 0 Td 49.259259 Tz (brain ) Tj 1.925 0 Td 47.222222 Tz (extract ) Tj 2.391667 0 Td 48.905724 Tz (endothelial ) Tj 3.655556 0 Td 43.75 Tz (cell ) Tj 1.497222 0 Td 56.712963 Tz (growth ) Tj 2.488889 0 Td 43.209877 Tz (kit ) Tj 1.205556 0 Td 62.87037 Tz (\(BBE\) ) Tj 51.428571 0 0 51.428571 0 1045.896797 Tm 25.316667 0 Td 57.992203 Tz (\(ATCC\)-supplemented ) Tj 6.883333 0 Td 72.106481 Tz (M200 ) Tj 1.963889 0 Td 47.530864 Tz (\(Invitrogen; ) Tj 3.694444 0 Td 51.131687 Tz (Carlsbad, ) Tj 3.033333 0 Td 44.444444 Tz (Calif.\) ) Tj 51.428571 0 0 51.428571 0 1004.164103 Tm 25.277778 0 Td 54.282407 Tz (with ) Tj 1.497222 0 Td 47.962963 Tz (daily ) Tj 1.672222 0 Td 64.814815 Tz (medium ) Tj 2.527778 0 Td 52.256944 Tz (changes. ) Tj 51.428571 0 0 51.428571 0 959.886978 Tm 26.036111 0 Td 57.253086 Tz (Simple ) Tj 2.158333 0 Td 55.416667 Tz (chemotaxis ) Tj 3.441667 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 76.157407 Tz (HUVECs ) Tj 2.897222 0 Td 60.493827 Tz (was ) Tj 1.225 0 Td 51.446759 Tz (assessed ) Tj 2.586111 0 Td 58.333333 Tz (on ) Tj 0.836111 0 Td 55.740741 Tz (upper ) Tj 1.75 0 Td 60.493827 Tz (and ) Tj 51.428571 0 0 51.428571 0 919.258373 Tm 25.297222 0 Td 55.740741 Tz (lower ) Tj 1.769444 0 Td 50 Tz (Surface ) Tj 2.236111 0 Td 50.987654 Tz (collagen-coated ) Tj 4.744444 0 Td 55.092593 Tz (8 ) Tj 0.525 0 Td 71.296296 Tz (um ) Tj 1.030556 0 Td 42.534722 Tz (inserts. ) Tj 2.216667 0 Td 62.87037 Tz (Lower ) Tj 2.002778 0 Td 49.907407 Tz (wells ) Tj 1.652778 0 Td 71.296296 Tz (con ) Tj 51.428571 0 0 51.428571 0 877.751174 Tm 25.277778 0 Td 50.231481 Tz (tained ) Tj 1.944444 0 Td 62.87037 Tz (M200. ) Tj 2.080556 0 Td 59.346065 Tz (BBE-supplemented ) Tj 5.833333 0 Td 64.166667 Tz (M200, ) Tj 2.080556 0 Td 50.231481 Tz (or ) Tj 0.758333 0 Td 94.791667 Tz (DMEM ) Tj 2.411111 0 Td 49.421296 Tz (material ) Tj 51.428571 0 0 51.428571 0 834.416459 Tm 25.297222 0 Td 45.775463 Tz (eluates. ) Tj 2.469444 0 Td 75.617284 Tz (HUVECs ) Tj 2.955556 0 Td 58.333333 Tz (were ) Tj 1.633333 0 Td 49.537037 Tz (starved ) Tj 2.294444 0 Td 51.131687 Tz (overnight ) Tj 2.975 0 Td 46.990741 Tz (in ) Tj 0.797222 0 Td 48.611111 Tz (0.5 ) Tj 1.127778 0 Td 71.296296 Tz (vol% ) Tj 1.925 0 Td 42.777778 Tz (fetal ) Tj 57.142857 0 0 57.142857 0 795.76196 Tm 22.75 0 Td 50.069444 Tz (bovine ) Tj 1.9075 0 Td 51.916667 Tz (serum ) Tj 1.6975 0 Td 50.231481 Tz (\(FBS\)-supplemented ) Tj 5.565 0 Td 64.895833 Tz (M200 ) Tj 1.645 0 Td 51.527778 Tz (and ) Tj 1.0325 0 Td 45.208333 Tz (plated ) Tj 1.6975 0 Td 40.833333 Tz (at ) Tj 0.595 0 Td 53.666667 Tz (5x10' ) Tj 51.428571 0 0 51.428571 0 751.404005 Tm 25.297222 0 Td 44.020062 Tz (cells/insert ) Tj 3.266667 0 Td 45.37037 Tz (in ) Tj 0.661111 0 Td 63.518519 Tz (M200. ) Tj 2.041667 0 Td 53.652263 Tz (Migration ) Tj 3.033333 0 Td 61.574074 Tz (was ) Tj 1.225 0 Td 54.62963 Tz (allowed ) Tj 2.391667 0 Td 48.611111 Tz (for ) Tj 0.972222 0 Td 58.333333 Tz (4 ) Tj 0.447222 0 Td 49.691358 Tz (hours. ) Tj 1.886111 0 Td 52.5 Tz (After ) Tj 51.428571 0 0 51.428571 0 709.84058 Tm 25.277778 0 Td 54.552469 Tz (which, ) Tj 2.119444 0 Td 44.074074 Tz (cells ) Tj 1.458333 0 Td 59.953704 Tz (on ) Tj 0.894444 0 Td 48.611111 Tz (the ) Tj 0.991667 0 Td 56.388889 Tz (upper ) Tj 1.788889 0 Td 45.37037 Tz (insert ) Tj 1.75 0 Td 49.421296 Tz (surfaces ) Tj 2.527778 0 Td 59.143519 Tz (were ) Tj 1.555556 0 Td 59.722222 Tz (removed ) Tj 2.663889 0 Td 52.662037 Tz (with ) Tj 1.419444 0 Td 55.092593 Tz (a ) Tj 51.428571 0 0 51.428571 0 670.431257 Tm 25.316667 0 Td 62.384259 Tz (PBS-dampened ) Tj 4.608333 0 Td 50.231481 Tz (cotton ) Tj 1.944444 0 Td 55.092593 Tz (Swab, ) Tj 1.75 0 Td 59.41358 Tz (and ) Tj 1.166667 0 Td 51.851852 Tz (those ) Tj 1.672222 0 Td 58.981481 Tz (which ) Tj 1.905556 0 Td 57.253086 Tz (had ) Tj 1.127778 0 Td 54.282407 Tz (migrated ) Tj 2.683333 0 Td 46.990741 Tz (to ) Tj 40 0 0 40 0 634 Tm 32.5 0 Td 65.277778 Tz (the ) Tj 1.325 0 Td 71.666667 Tz (lower ) Tj 2.275 0 Td 64.285714 Tz (Surface ) Tj 2.875 0 Td 75 Tz (were ) Tj 1.95 0 Td 66.666667 Tz (cold ) Tj 1.775 0 Td 72.619048 Tz (shocked ) Tj 3.225 0 Td 68.75 Tz (with ) Tj 1.825 0 Td 76.041667 Tz (0C. ) Tj 2.075 0 Td 88.888889 Tz (PBS ) Tj 1.8 0 Td 73.611111 Tz (and ) Tj 1.5 0 Td 61.666667 Tz (fixed ) Tj 40 0 0 40 0 592 Tm 32.5 0 Td 69.791667 Tz (with ) Tj 1.925 0 Td 75 Tz (-20 ) Tj 2.05 0 Td 68.75 Tz (C. ) Tj 1.15 0 Td 71.354167 Tz (methanol ) Tj 3.675 0 Td 75 Tz (on ) Tj 1.175 0 Td 53.125 Tz (ice. ) Tj 1.6 0 Td 69.166667 Tz (Fixed ) Tj 2.275 0 Td 57.5 Tz (cells ) Tj 2 0 Td 75 Tz (were ) Tj 2.05 0 Td 64.285714 Tz (labeled ) Tj 2.95 0 Td 67.708333 Tz (with ) Tj 51.428571 0 0 51.428571 0 543.73494 Tm 25.375 0 Td 55.092593 Tz (10 ) Tj 0.855556 0 Td 61.898148 Tz (uMethidium ) Tj 3.908333 0 Td 58.922559 Tz (homodimer-1 ) Tj 4.141667 0 Td 54.6875 Tz (\(EthD-1\) ) Tj 2.780556 0 Td 48.611111 Tz (\(Invitrogen\) ) Tj 3.694444 0 Td 42.12963 Tz (in ) Tj 0.738889 0 Td 69.135802 Tz (PBS ) Tj 51.428571 0 0 51.428571 0 504.623932 Tm 25.297222 0 Td 58.333333 Tz (and ) Tj 1.283333 0 Td 59.41358 Tz (imaged ) Tj 2.352778 0 Td 53.148148 Tz (using ) Tj 1.808333 0 Td 50.771605 Tz (fluorescence ) Tj 3.888889 0 Td 52.73569 Tz (microscopy. ) Tj 3.752778 0 Td 56.712963 Tz (Four ) Tj 1.575 0 Td 54.552469 Tz (frames ) Tj 2.216667 0 Td 56.712963 Tz (of ) Tj 51.428571 0 0 51.428571 0 461.508475 Tm 25.297222 0 Td 56.712963 Tz (view ) Tj 1.575 0 Td 54.012346 Tz (\(100x\) ) Tj 2.1 0 Td 52.932099 Tz (per ) Tj 1.108333 0 Td 44.290123 Tz (insert ) Tj 1.75 0 Td 59.143519 Tz (were ) Tj 1.555556 0 Td 52.256944 Tz (captured ) Tj 2.644444 0 Td 59.41358 Tz (and ) Tj 1.205556 0 Td 44.074074 Tz (cells ) Tj 1.477778 0 Td 55.092593 Tz (counted ) Tj 2.469444 0 Td 52.5 Tz (using ) Tj 51.428571 0 0 51.428571 0 421.271868 Tm 25.316667 0 Td 47.800926 Tz (FIJI/Image.J ) Tj 3.636111 0 Td 51.851852 Tz (software ) Tj 2.625 0 Td 57.037037 Tz (\(NIH; ) Tj 1.925 0 Td 52.211934 Tz (Bethesda, ) Tj 2.975 0 Td 43.209877 Tz (N.J.\). ) Tj 1.730556 0 Td 63.734568 Tz (The ) Tj 1.283333 0 Td 54.768519 Tz (experiment ) Tj 51.428571 0 0 51.428571 0 377.68 Tm 25.277778 0 Td 62.654321 Tz (was ) Tj 1.341667 0 Td 51.041667 Tz (repeated ) Tj 2.625 0 Td 46.990741 Tz (to ) Tj 0.777778 0 Td 53.240741 Tz (confirm ) Tj 2.411111 0 Td 49.151235 Tz 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0.885294 0 Td 36.029412 Tz (it ) Tj 0.658824 0 Td 51.89951 Tz (diffused ) Tj 2.697059 0 Td 61.764706 Tz (from ) Tj 1.75 0 Td 66.339869 Tz (F1+ ) Tj 1.4 0 Td 64.052288 Tz (and ) Tj 1.4 0 Td 57.47549 Tz (F2+: ) Tj 51.428571 0 0 51.428571 0 1835.990291 Tm 6.358333 0 Td 50.05144 Tz (depletion ) Tj 2.877778 0 Td 56.712963 Tz (of ) Tj 0.836111 0 Td 75.347222 Tz (PDGF ) Tj 2.002778 0 Td 46.990741 Tz (in ) Tj 0.719444 0 Td 49.691358 Tz (the ) Tj 1.069444 0 Td 52.391975 Tz (experimental ) Tj 3.966667 0 Td 50.555556 Tz (setup ) Tj 1.730556 0 Td 60.493827 Tz (was ) Tj 1.302778 0 Td 55.452675 Tz (monitored ) Tj 51.428571 0 0 51.428571 0 1794.835206 Tm 6.377778 0 Td 46.254209 Tz (separately, ) Tj 3.305556 0 Td 53.703704 Tz (through ) Tj 2.508333 0 Td 51.388889 Tz (spiking ) Tj 2.372222 0 Td 49.074074 Tz (eluents ) Tj 2.333333 0 Td 52.662037 Tz (with ) Tj 1.536111 0 Td 62.87037 Tz (hCPR. ) Tj 2.216667 0 Td 55.092593 Tz (Both ) Tj 1.594444 0 Td 65.625 Tz (accu ) Tj 51.428571 0 0 51.428571 0 1751.066934 Tm 6.358333 0 Td 53.472222 Tz (mulation ) Tj 2.936111 0 Td 61.574074 Tz (and ) Tj 1.458333 0 Td 49.691358 Tz (depletion ) Tj 3.091667 0 Td 58.333333 Tz (were ) Tj 1.788889 0 Td 55.092593 Tz (monitored ) Tj 3.325 0 Td 55.092593 Tz (over ) Tj 1.672222 0 Td 51.851852 Tz (a ) Tj 0.680556 0 Td 45.37037 Tz (five ) Tj 1.458333 0 Td 59.41358 Tz (day ) Tj 51.428571 0 0 51.428571 0 1710.210393 Tm 6.358333 0 Td 48.148148 Tz (period. ) Tj 2.255556 0 Td 58.333333 Tz (Based ) Tj 1.963889 0 Td 58.333333 Tz (on ) Tj 0.894444 0 Td 58.333333 Tz (5 ) Tj 0.525 0 Td 60.493827 Tz (and ) Tj 1.283333 0 Td 59.953704 Tz (24 ) Tj 0.875 0 Td 57.523148 Tz (hour ) Tj 1.536111 0 Td 46.666667 Tz (data, ) Tj 1.613889 0 Td 62.654321 Tz (F1+ ) Tj 1.322222 0 Td 52.777778 Tz (appears ) Tj 2.430556 0 Td 45.37037 Tz (to ) Tj 0.738889 0 Td 48.611111 Tz (release ) Tj 51.428571 0 0 51.428571 0 1668.544994 Tm 6.358333 0 Td 56.17284 Tz (growth ) Tj 2.275 0 Td 46.759259 Tz (factors ) Tj 2.177778 0 Td 45.37037 Tz (at ) Tj 0.738889 0 Td 58.333333 Tz (an ) Tj 0.933333 0 Td 49.789562 Tz (accelerated ) Tj 3.5 0 Td 45.37037 Tz (rate ) Tj 1.322222 0 Td 59.548611 Tz (compared ) Tj 3.091667 0 Td 43.75 Tz (to ) Tj 0.797222 0 Td 54.282407 Tz (F2+: ) Tj 1.575 0 Td 43.75 Tz (this ) Tj 52.857143 0 0 52.857143 0 1625.714286 Tm 6.186486 0 Td 49.5905 Tz (observation ) Tj 3.518919 0 Td 39.414414 Tz (is ) Tj 0.718919 0 Td 44.594595 Tz (in-line ) Tj 2.062162 0 Td 52.815315 Tz (with ) Tj 1.57027 0 Td 50.45045 Tz (F1 ) Tj 0.7 0 Td 59.90991 Tz (+ ) Tj 0.586486 0 Td 54.129129 Tz (having ) Tj 2.156757 0 Td 56.756757 Tz (a ) Tj 0.586486 0 Td 53.603604 Tz (lower ) Tj 1.778378 0 Td 54.129129 Tz (weight ) Tj 2.175676 0 Td 63.063063 Tz (per ) Tj 51.428571 0 0 51.428571 0 1588.152258 Tm 6.358333 0 Td 53.703704 Tz (centage ) Tj 2.527778 0 Td 58.333333 Tz (of ) Tj 0.933333 0 Td 47.800926 Tz (alginate ) Tj 2.625 0 Td 59.548611 Tz (compared ) Tj 3.169444 0 Td 45.37037 Tz (to ) Tj 0.894444 0 Td 55.092593 Tz (F2+, ) Tj 1.633333 0 Td 53.596866 Tz (corresponding ) Tj 4.452778 0 Td 48.611111 Tz (to ) Tj 0.913889 0 Td 55.092593 Tz (a ) Tj 51.428571 0 0 51.428571 0 1541.669654 Tm 6.358333 0 Td 55.092593 Tz (lower ) Tj 2.1 0 Td 47.530864 Tz (diffusion ) Tj 3.033333 0 Td 44.560185 Tz (coefficient. ) Tj 3.675 0 Td 48.021886 Tz (Significant ) Tj 3.616667 0 Td 48.021886 Tz (differences ) Tj 3.636111 0 Td 59.143519 Tz (were ) Tj 51.428571 0 0 51.428571 0 1501.498765 Tm 6.358333 0 Td 57.037037 Tz (found ) Tj 1.983333 0 Td 65.625 Tz (when ) Tj 1.886111 0 Td 48.611111 Tz (individual ) Tj 3.227778 0 Td 56.712963 Tz (specimen ) Tj 3.013889 0 Td 45.775463 Tz (profiles ) Tj 2.488889 0 Td 59.953704 Tz (were ) Tj 1.711111 0 Td 53.148148 Tz (considered ) Tj 51.428571 0 0 51.428571 0 1459.742293 Tm 6.377778 0 Td 53.796296 Tz (\(p=0.0027\) ) Tj 3.461111 0 Td 58.333333 Tz (and ) Tj 1.302778 0 Td 51.851852 Tz (support ) Tj 2.430556 0 Td 55.092593 Tz (F1+s ) Tj 1.886111 0 Td 49.789562 Tz (accelerated ) Tj 3.519444 0 Td 48.148148 Tz (release ) Tj 2.255556 0 Td 43.75 Tz (rate ) Tj 1.322222 0 Td 54.282407 Tz (with ) Tj 51.428571 0 0 51.428571 0 1419.295758 Tm 6.338889 0 Td 60.493827 Tz (two ) Tj 1.302778 0 Td 62.654321 Tz (F2+ ) Tj 1.302778 0 Td 55.812757 Tz (specimens ) Tj 3.188889 0 Td 48.148148 Tz (eluting ) Tj 2.197222 0 Td 45.868946 Tz (significantly ) Tj 3.772222 0 Td 43.75 Tz (less ) Tj 1.263889 0 Td 76.157407 Tz (PDGF ) Tj 1.983333 0 Td 51.851852 Tz (than ) Tj 1.4 0 Td 60.493827 Tz (one ) Tj 51.428571 0 0 51.428571 0 1377.012107 Tm 6.377778 0 Td 56.712963 Tz (F1 ) Tj 0.758333 0 Td 64.814815 Tz (+ ) Tj 0.563889 0 Td 53.652263 Tz (specimen. ) Tj 3.033333 0 Td 57.407407 Tz (Average ) Tj 2.586111 0 Td 50.231481 Tz (values ) Tj 1.944444 0 Td 50.771605 Tz (are ) Tj 1.088889 0 Td 48.971193 Tz (indicated ) Tj 2.780556 0 Td 61.574074 Tz (by ) Tj 0.952778 0 Td 54.166667 Tz (markers ) Tj 2.45 0 Td 54.282407 Tz (with ) Tj 51.428571 0 0 51.428571 0 1332.618454 Tm 6.358333 0 Td 50.231481 Tz (plus ) Tj 1.419444 0 Td 58.333333 Tz (and ) Tj 1.302778 0 Td 57.685185 Tz (minus ) Tj 1.944444 0 Td 58.333333 Tz (one ) Tj 1.263889 0 Td 51.446759 Tz (standard ) Tj 2.663889 0 Td 49.691358 Tz (deviation ) Tj 2.877778 0 Td 48.611111 Tz (error ) Tj 1.613889 0 Td 51.041667 Tz (bars ) Tj 1.458333 0 Td 62.222222 Tz (shown ) Tj 2.1 0 Td 47.530864 Tz (for ) Tj 51.428571 0 0 51.428571 0 1295.010959 Tm 6.377778 0 Td 63.734568 Tz (F1+ ) Tj 1.322222 0 Td 59.41358 Tz (and ) Tj 1.263889 0 Td 55.092593 Tz (F2+, ) Tj 1.555556 0 Td 45.619658 Tz (respectively. ) Tj 51.428571 0 0 51.428571 0 1251.007884 Tm 7.116667 0 Td 51.203704 Tz (Early ) Tj 1.730556 0 Td 48.148148 Tz (release ) Tj 2.236111 0 Td 49.691358 Tz (values ) Tj 1.963889 0 Td 41.049383 Tz (\(5. ) Tj 0.991667 0 Td 48.611111 Tz (24, ) Tj 1.069444 0 Td 59.41358 Tz (and ) Tj 1.225 0 Td 59.953704 Tz (48 ) Tj 0.875 0 Td 49.074074 Tz (hours\); ) Tj 2.275 0 Td 51.851852 Tz (Suggest ) Tj 2.352778 0 Td 55.092593 Tz (a ) Tj 0.525 0 Td 61.574074 Tz (more ) Tj 51.428571 0 0 51.428571 0 1210.481074 Tm 6.377778 0 Td 50.05144 Tz (Sustained ) Tj 2.877778 0 Td 48.148148 Tz (release ) Tj 2.236111 0 Td 57.523148 Tz (from ) Tj 1.594444 0 Td 55.902778 Tz (F2+, ) Tj 1.536111 0 Td 49.691358 Tz (the ) Tj 1.069444 0 Td 55.092593 Tz (formula ) Tj 2.527778 0 Td 50.87963 Tz (containing ) Tj 3.227778 0 Td 55.092593 Tz (a ) Tj 0.525 0 Td 52.391975 Tz (higher ) Tj 51.428571 0 0 51.428571 0 1171.274725 Tm 6.338889 0 Td 56.17284 Tz (weight ) Tj 2.197222 0 Td 52.5 Tz (percentage ) Tj 3.363889 0 Td 55.092593 Tz (of ) Tj 0.758333 0 Td 45.730453 Tz (alginate, ) Tj 2.683333 0 Td 50.231481 Tz (as ) Tj 0.816667 0 Td 59.548611 Tz (compared ) Tj 3.013889 0 Td 48.611111 Tz (to ) Tj 0.797222 0 Td 55.092593 Tz (F1 ) Tj 0.758333 0 Td 45.37037 Tz (+. ) Tj 0.738889 0 Td 56.481481 Tz (Without ) Tj 51.428571 0 0 51.428571 0 1127.124324 Tm 6.338889 0 Td 54.282407 Tz (temporal ) Tj 3.227778 0 Td 48.37963 Tz (consideration, ) Tj 4.705556 0 Td 56.712963 Tz (no ) Tj 1.302778 0 Td 45.664983 Tz (significant ) Tj 3.597222 0 Td 48.935185 Tz (difference ) Tj 3.558333 0 Td 60.493827 Tz (was ) Tj 51.428571 0 0 51.428571 0 1084.817751 Tm 6.358333 0 Td 50.636574 Tz (detected ) Tj 2.566667 0 Td 57.407407 Tz (between ) Tj 2.586111 0 Td 48.611111 Tz (the ) Tj 1.011111 0 Td 47.314815 Tz (materials, ) Tj 2.955556 0 Td 51.851852 Tz (but ) Tj 1.069444 0 Td 44.444444 Tz (alternatively, ) Tj 3.927778 0 Td 49.691358 Tz (values ) Tj 1.944444 0 Td 59.143519 Tz (were ) Tj 51.428571 0 0 51.428571 0 1044.680608 Tm 6.358333 0 Td 57.037037 Tz (found ) Tj 2.002778 0 Td 45.37037 Tz (to ) Tj 0.875 0 Td 49.421296 Tz (increase ) Tj 2.683333 0 Td 52.662037 Tz (with ) Tj 1.594444 0 Td 53.472222 Tz (time ) Tj 1.594444 0 Td 51.557239 Tz (\(p=0.0033\). ) Tj 3.694444 0 Td 50.771605 Tz (Additionally ) Tj 3.947222 0 Td 61.574074 Tz (and ) Tj 51.428571 0 0 51.428571 0 1000.970273 Tm 6.338889 0 Td 54.282407 Tz (with ) Tj 1.438889 0 Td 64.00463 Tz (some ) Tj 1.652778 0 Td 48.905724 Tz (insinuation ) Tj 3.363889 0 Td 54.444444 Tz (about ) Tj 1.788889 0 Td 61.574074 Tz (F2+ ) Tj 1.244444 0 Td 55.092593 Tz (having ) Tj 2.080556 0 Td 55.092593 Tz (a ) Tj 0.447222 0 Td 54.552469 Tz (slower ) Tj 2.061111 0 Td 48.148148 Tz (release ) Tj 2.138889 0 Td 41.481481 Tz (rate, ) Tj 51.428571 0 0 51.428571 0 963.488372 Tm 6.358333 0 Td 51.041667 Tz (variance ) Tj 2.547222 0 Td 66.759259 Tz (among ) Tj 2.119444 0 Td 55.812757 Tz (specimens ) Tj 3.15 0 Td 61.574074 Tz (was ) Tj 1.244444 0 Td 56.388889 Tz (found ) Tj 1.808333 0 Td 46.254209 Tz (significant ) Tj 3.169444 0 Td 51.557239 Tz (\(p=0.0027\). ) Tj 51.428571 0 0 51.428571 0 922.57346 Tm 6.377778 0 Td 54.503367 Tz (Independent ) Tj 3.733333 0 Td 51.851852 Tz (post-hoc ) Tj 2.663889 0 Td 54.6875 Tz (grouping ) Tj 2.8 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 56.712963 Tz (specimen ) Tj 2.877778 0 Td 59.41358 Tz (and ) Tj 1.205556 0 Td 54.282407 Tz (time ) Tj 1.477778 0 Td 46.296296 Tz (factors ) Tj 51.428571 0 0 51.428571 0 878.263158 Tm 6.377778 0 Td 61.033951 Tz (showed ) Tj 2.333333 0 Td 59.41358 Tz (two ) Tj 1.263889 0 Td 62.654321 Tz (F2+ ) Tj 1.283333 0 Td 56.17284 Tz (specimens ) Tj 3.227778 0 Td 49.016204 Tz (released ) Tj 2.488889 0 Td 46.118234 Tz (significantly ) Tj 3.752778 0 Td 44.560185 Tz (less ) Tj 1.263889 0 Td 75.347222 Tz (PDGF ) Tj 51.428571 0 0 51.428571 0 834.37931 Tm 6.338889 0 Td 52.662037 Tz (than ) Tj 1.458333 0 Td 58.333333 Tz (one ) Tj 1.263889 0 Td 61.574074 Tz (F1+ ) Tj 1.322222 0 Td 56.712963 Tz (specimen ) Tj 2.877778 0 Td 59.41358 Tz (and ) Tj 1.244444 0 Td 58.333333 Tz (5 ) Tj 0.525 0 Td 56.712963 Tz (hour ) Tj 1.555556 0 Td 76.967593 Tz (PDGF ) Tj 2.022222 0 Td 54.282407 Tz (accumulation ) Tj 4.102778 0 Td 61.574074 Tz (was ) Tj 51.428571 0 0 51.428571 0 795.935657 Tm 6.377778 0 Td 45.619658 Tz (significantly ) Tj 3.752778 0 Td 55.740741 Tz (lower ) Tj 1.847222 0 Td 51.041667 Tz (than ) Tj 1.438889 0 Td 45.37037 Tz (that ) Tj 1.244444 0 Td 45.37037 Tz (at ) Tj 0.797222 0 Td 57.253086 Tz (120 ) Tj 1.166667 0 Td 50.231481 Tz (hours, ) Tj 2.022222 0 Td 45.868946 Tz (respectively. ) Tj 51.428571 0 0 51.428571 0 754.48329 Tm 7.097222 0 Td 75.617284 Tz (Now ) Tj 1.613889 0 Td 46.450617 Tz (referring ) Tj 2.702778 0 Td 43.75 Tz (to ) Tj 0.758333 0 Td 54.282407 Tz (FIG. ) Tj 1.497222 0 Td 58.333333 Tz (5 ) Tj 0.563889 0 Td 48.611111 Tz (the ) Tj 1.05 0 Td 50.05144 Tz (depletion ) Tj 2.897222 0 Td 54.444444 Tz (experiment ) Tj 3.441667 0 Td 62.114198 Tz (showed ) Tj 51.428571 0 0 51.428571 0 710.726098 Tm 6.358333 0 Td 55.092593 Tz (a ) Tj 0.602778 0 Td 45.37037 Tz (loss ) Tj 1.361111 0 Td 59.953704 Tz (of ) Tj 0.894444 0 Td 76.967593 Tz (PDGF ) Tj 2.119444 0 Td 52.662037 Tz (with ) Tj 1.575 0 Td 53.472222 Tz (time ) Tj 1.555556 0 Td 54.12037 Tz (\(p<0.0001\) ) Tj 3.5 0 Td 58.333333 Tz (which ) Tj 2.022222 0 Td 62.654321 Tz (was ) Tj 1.438889 0 Td 49.826389 Tz (indistin ) Tj 51.428571 0 0 51.428571 0 671.009479 Tm 6.358333 0 Td 51.851852 Tz (guishable ) Tj 2.975 0 Td 57.87037 Tz (between ) Tj 2.625 0 Td 52.824074 Tz (specimens. ) Tj 3.383333 0 Td 63.734568 Tz (The ) Tj 1.302778 0 Td 53.703704 Tz (average ) Tj 2.45 0 Td 50.356125 Tz (concentration ) Tj 4.141667 0 Td 56.712963 Tz (of ) Tj 51.428571 0 0 51.428571 0 629.051546 Tm 6.338889 0 Td 50.771605 Tz (the ) Tj 1.088889 0 Td 52.932099 Tz (spiked ) Tj 2.061111 0 Td 49.074074 Tz (eluents ) Tj 2.255556 0 Td 59.41358 Tz (had ) Tj 1.263889 0 Td 50.636574 Tz (depleted ) Tj 2.644444 0 Td 71.296296 Tz (65% ) Tj 1.497222 0 Td 58.333333 Tz (by ) Tj 0.972222 0 Td 58.333333 Tz (120 ) Tj 1.186111 0 Td 49.691358 Tz (hours. ) Tj 51.428571 0 0 51.428571 0 586.899128 Tm 7.097222 0 Td 75.617284 Tz (Now ) Tj 1.516667 0 Td 47.170782 Tz (referring ) Tj 2.663889 0 Td 43.75 Tz (to ) Tj 0.680556 0 Td 57.037037 Tz (FIGS. ) Tj 1.886111 0 Td 72.916667 Tz (6A ) Tj 0.952778 0 Td 93.981481 Tz (& ) Tj 0.738889 0 Td 55.092593 Tz (6B, ) Tj 1.127778 0 Td 57.613169 Tz (monocytes ) Tj 3.266667 0 Td 54.552469 Tz (demonstrated ) Tj 51.428571 0 0 51.428571 0 546.412811 Tm 6.358333 0 Td 51.203704 Tz (inflammatory-related ) Tj 6.436111 0 Td 51.635802 Tz (chemoattractant ) Tj 4.919444 0 Td 48.287037 Tz (properties ) Tj 3.208333 0 Td 56.712963 Tz (of ) Tj 0.875 0 Td 48.148148 Tz (eluates ) Tj 51.428571 0 0 51.428571 0 504.385343 Tm 6.358333 0 Td 58.333333 Tz (from ) Tj 1.536111 0 Td 53.472222 Tz (both ) Tj 1.419444 0 Td 52.391975 Tz (experimental ) Tj 3.927778 0 Td 59.41358 Tz (and ) Tj 1.225 0 Td 49.016204 Tz (material ) Tj 2.469444 0 Td 49.074074 Tz (control ) Tj 2.216667 0 Td 53.148148 Tz (specimens. ) Tj 3.344444 0 Td 73.45679 Tz (74% ) Tj 51.428571 0 0 51.428571 0 462.039164 Tm 6.358333 0 Td 58.333333 Tz (and ) Tj 1.341667 0 Td 73.45679 Tz (83% ) Tj 1.613889 0 Td 59.953704 Tz (of ) Tj 0.913889 0 Td 55.092593 Tz (seeded ) Tj 2.294444 0 Td 57.253086 Tz (monocytes ) Tj 3.402778 0 Td 59.41358 Tz (had ) Tj 1.380556 0 Td 53.472222 Tz (migrated ) Tj 2.858333 0 Td 45.37037 Tz (to ) Tj 0.836111 0 Td 49.691358 Tz (the ) Tj 1.205556 0 Td 55.092593 Tz (lower ) Tj 51.428571 0 0 51.428571 0 421.865707 Tm 6.338889 0 Td 51.851852 Tz (wells ) Tj 1.711111 0 Td 50.87963 Tz (containing ) Tj 3.208333 0 Td 63.734568 Tz (F1+ ) Tj 1.263889 0 Td 59.41358 Tz (and ) Tj 1.244444 0 Td 62.654321 Tz (F2+ ) Tj 1.263889 0 Td 48.148148 Tz (eluates ) Tj 2.177778 0 Td 45.37037 Tz (in ) Tj 0.661111 0 Td 50.771605 Tz (the ) Tj 1.05 0 Td 54.012346 Tz (simple ) Tj 2.1 0 Td 52.572016 Tz (migration ) Tj 51.428571 0 0 51.428571 0 380.109916 Tm 6.358333 0 Td 46.990741 Tz (assay, ) Tj 1.905556 0 Td 45.868946 Tz (respectively. ) Tj 3.791667 0 Td 54.282407 Tz (FIG. ) Tj 1.516667 0 Td 72.916667 Tz (6A ) Tj 1.05 0 Td 59.62963 Tz (shows ) Tj 2.002778 0 Td 57.253086 Tz (monocytes ) Tj 3.286111 0 Td 54.822531 Tz (demonstrated ) Tj 51.428571 0 0 51.428571 0 339.412811 Tm 6.358333 0 Td 51.203704 Tz (inflammatory-related ) Tj 6.436111 0 Td 51.635802 Tz (chemoattractant ) Tj 4.919444 0 Td 48.287037 Tz (properties ) Tj 3.208333 0 Td 56.712963 Tz (of ) Tj 0.875 0 Td 48.148148 Tz (eluates ) Tj 51.428571 0 0 51.428571 0 296.750953 Tm 6.358333 0 Td 58.333333 Tz (from ) Tj 1.730556 0 Td 53.472222 Tz (both ) Tj 1.652778 0 Td 52.662037 Tz (experimental ) Tj 4.122222 0 Td 59.41358 Tz (and ) Tj 1.458333 0 Td 48.611111 Tz (material ) Tj 2.702778 0 Td 48.611111 Tz (control ) Tj 2.391667 0 Td 51.262626 Tz (speciments. ) Tj 51.428571 0 0 51.428571 0 254.654438 Tm 6.377778 0 Td 70.486111 Tz (RAW264.7 ) Tj 3.558333 0 Td 53.148148 Tz (clone ) Tj 1.711111 0 Td 57.253086 Tz (TIB-71 ) Tj 2.294444 0 Td 62.87037 Tz (mouse ) Tj 2.061111 0 Td 59.143519 Tz (monocyte ) Tj 2.994444 0 Td 52.211934 Tz (migration ) Tj 2.975 0 Td 51.311728 Tz (assays ) Tj 51.428571 0 0 51.428571 0 211.628916 Tm 6.358333 0 Td 44.907407 Tz (results ) Tj 2.022222 0 Td 50.771605 Tz (are ) Tj 1.05 0 Td 56.17284 Tz (shown. ) Tj 2.216667 0 Td 55.092593 Tz (F1+, ) Tj 1.477778 0 Td 55.092593 Tz (F2+, ) Tj 1.497222 0 Td 62.654321 Tz (F1- ) Tj 1.263889 0 Td 59.41358 Tz (and ) Tj 1.205556 0 Td 60.853909 Tz (F2-groups ) Tj 3.422222 0 Td 47.800926 Tz (indicate ) Tj 2.430556 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 173.026713 Tm 6.338889 0 Td 55.092593 Tz (use ) Tj 1.186111 0 Td 56.712963 Tz (of ) Tj 0.797222 0 Td 49.421296 Tz (material ) Tj 2.566667 0 Td 45.37037 Tz (eluates. ) Tj 2.411111 0 Td 53.128507 Tz (n-formyl-methionyl-leucyl-phenyla ) Tj 30 0 0 30 0 2547 Tm 41.666667 0 Td 75 Tz (10 ) Tj 30 0 0 30 0 2339 Tm 41.666667 0 Td 72.222222 Tz (15 ) Tj 30 0 0 30 0 1923 Tm 41.6 0 Td 77.777778 Tz (25 ) Tj 30 0 0 30 0 1716 Tm 41.6 0 Td 80.555556 Tz (30 ) Tj 30 0 0 30 0 1507 Tm 41.6 0 Td 77.777778 Tz (35 ) Tj 30 0 0 30 0 1299 Tm 41.566667 0 Td 83.333333 Tz (40 ) Tj 30 0 0 30 0 1091 Tm 41.566667 0 Td 80.555556 Tz (45 ) Tj 30 0 0 30 0 883 Tm 41.6 0 Td 80.555556 Tz (50 ) Tj 30 0 0 30 0 675 Tm 41.6 0 Td 77.777778 Tz (55 ) Tj 30 0 0 30 0 467 Tm 41.633333 0 Td 77.777778 Tz (60 ) Tj 30 0 0 30 0 259 Tm 41.633333 0 Td 75 Tz (65 ) Tj 52.857143 0 0 52.857143 0 2968 Tm 32.635135 0 Td 77.252252 Tz (20 ) Tj 51.428571 0 0 51.428571 0 2914.170972 Tm 25.297222 0 Td 49.691358 Tz (lanine ) Tj 1.983333 0 Td 61.033951 Tz (\(fMLP\) ) Tj 2.391667 0 Td 61.574074 Tz (was ) Tj 1.341667 0 Td 55.092593 Tz (used ) Tj 1.477778 0 Td 51.851852 Tz (as ) Tj 0.797222 0 Td 51.851852 Tz (a ) Tj 0.505556 0 Td 48.206019 Tz (positive ) Tj 2.488889 0 Td 53.030303 Tz (chemotactic ) Tj 3.675 0 Td 49.537037 Tz (control ) Tj 2.255556 0 Td 46.990741 Tz (at ) Tj 57.142857 0 0 57.142857 0 2875.532194 Tm 22.82 0 Td 52.5 Tz (10 ) Tj 1.4525 0 Td 93.333333 Tz (M ) Tj 0.875 0 Td 43.75 Tz (unsuccessfully, ) Tj 4.235 0 Td 53.472222 Tz (and ) Tj 1.2775 0 Td 49.291667 Tz (Dulbecco's ) Tj 3.255 0 Td 48.489583 Tz (modified ) Tj 2.625 0 Td 46.25 Tz (Eagle's ) Tj 58.571429 0 0 58.571429 0 2831.382064 Tm 22.212195 0 Td 57.859079 Tz (medium ) Tj 2.219512 0 Td 67.344173 Tz (\(DMEM\) ) Tj 2.543902 0 Td 55.96206 Tz (was ) Tj 1.126829 0 Td 49.796748 Tz (used ) Tj 1.314634 0 Td 44.105691 Tz (as ) Tj 0.64878 0 Td 51.219512 Tz (a ) Tj 0.426829 0 Td 44.461382 Tz (negative ) Tj 2.253659 0 Td 40.54878 Tz (control. ) Tj 2.168293 0 Td 51.219512 Tz (10 ) Tj 0.973171 0 Td 69.004065 Tz (mono ) Tj 51.428571 0 0 51.428571 0 2790.653113 Tm 25.297222 0 Td 49.259259 Tz (cytes ) Tj 1.788889 0 Td 58.333333 Tz (were ) Tj 1.652778 0 Td 53.472222 Tz (placed ) Tj 2.197222 0 Td 46.990741 Tz (in ) Tj 0.875 0 Td 48.611111 Tz (the ) Tj 1.166667 0 Td 55.740741 Tz (upper ) Tj 1.925 0 Td 44.444444 Tz (inserts ) Tj 2.177778 0 Td 59.953704 Tz (of ) Tj 0.855556 0 Td 80.046296 Tz (TRANSWELLS ) Tj 51.428571 0 0 51.428571 0 2749.759281 Tm 25.316667 0 Td 47.395833 Tz (\(CoStar, ) Tj 2.45 0 Td 56.712963 Tz (Cambridge, ) Tj 3.577778 0 Td 50.462963 Tz (Mass.\). ) Tj 2.294444 0 Td 60.493827 Tz (and ) Tj 1.244444 0 Td 54.62963 Tz (allowed ) Tj 2.45 0 Td 46.990741 Tz (to ) Tj 0.777778 0 Td 52.314815 Tz (migrate ) Tj 2.352778 0 Td 54.62963 Tz (towards ) Tj 51.428571 0 0 51.428571 0 2707.152566 Tm 25.297222 0 Td 52.391975 Tz (experimental ) Tj 3.947222 0 Td 59.41358 Tz (and ) Tj 1.225 0 Td 49.537037 Tz (control ) Tj 2.294444 0 Td 47.890947 Tz (solutions ) Tj 2.780556 0 Td 46.990741 Tz (in ) Tj 0.758333 0 Td 49.691358 Tz (the ) Tj 1.088889 0 Td 55.092593 Tz (lower ) Tj 1.808333 0 Td 46.666667 Tz (well. ) Tj 51.428571 0 0 51.428571 0 2664.984456 Tm 26.036111 0 Td 57.523148 Tz (Both ) Tj 1.594444 0 Td 51.311728 Tz (assays ) Tj 2.080556 0 Td 54.552469 Tz (\(FIGS. ) Tj 2.236111 0 Td 69.675926 Tz (6A ) Tj 1.05 0 Td 58.333333 Tz (and ) Tj 1.283333 0 Td 59.41358 Tz (6B\) ) Tj 1.263889 0 Td 53.914141 Tz (demonstrate ) Tj 3.752778 0 Td 50.771605 Tz (the ) Tj 1.088889 0 Td 49.826389 Tz (inherent ) Tj 51.428571 0 0 51.428571 0 2626.36534 Tm 25.297222 0 Td 50.771605 Tz (nature ) Tj 1.944444 0 Td 53.472222 Tz (of ) Tj 0.719444 0 Td 47.530864 Tz (the ) Tj 0.972222 0 Td 48.302469 Tz (alginate/B-tricalcium ) Tj 6.202778 0 Td 55.092593 Tz (phosphate ) Tj 3.111111 0 Td 56.17284 Tz (raw ) Tj 1.186111 0 Td 48.971193 Tz (materials ) Tj 2.761111 0 Td 46.990741 Tz (to ) Tj 51.428571 0 0 51.428571 0 2582.593452 Tm 25.297222 0 Td 54.012346 Tz (induce ) Tj 2.411111 0 Td 63.194444 Tz (some ) Tj 1.983333 0 Td 59.143519 Tz (monocyte ) Tj 3.286111 0 Td 46.990741 Tz (activation ) Tj 3.286111 0 Td 58.333333 Tz (\(F1- ) Tj 1.827778 0 Td 58.333333 Tz (and ) Tj 1.536111 0 Td 58.333333 Tz (F2-\) ) Tj 1.866667 0 Td 53.472222 Tz (with ) Tj 51.428571 0 0 51.428571 0 2542.749693 Tm 25.297222 0 Td 57.928241 Tz (enhanced ) Tj 2.994444 0 Td 43.518519 Tz (ability ) Tj 2.080556 0 Td 53.796296 Tz (being ) Tj 1.886111 0 Td 60.925926 Tz (shown ) Tj 2.1 0 Td 48.611111 Tz (for ) Tj 1.069444 0 Td 50.771605 Tz (the ) Tj 1.127778 0 Td 51.103989 Tz (experimental, ) Tj 4.2 0 Td 67.407407 Tz (human ) Tj 51.428571 0 0 51.428571 0 2500.815242 Tm 25.297222 0 Td 51.58179 Tz (concentrated ) Tj 3.811111 0 Td 45.37037 Tz (platelet ) Tj 2.313889 0 Td 48.773148 Tz (releasate-containing ) Tj 6.008333 0 Td 48.251029 Tz (materials ) Tj 2.741667 0 Td 58.333333 Tz (\(F1+ ) Tj 1.536111 0 Td 60.493827 Tz (and ) Tj 51.428571 0 0 51.428571 0 2456 Tm 25.316667 0 Td 51.203704 Tz (F2+\). ) Tj 51.428571 0 0 51.428571 0 2417.56129 Tm 26.016667 0 Td 75.617284 Tz (Now ) Tj 1.613889 0 Td 46.8107 Tz (referring ) Tj 2.722222 0 Td 43.75 Tz (to ) Tj 0.758333 0 Td 53.472222 Tz (FIG. ) Tj 1.516667 0 Td 56.17284 Tz (6B, ) Tj 1.186111 0 Td 47.685185 Tz (eluates ) Tj 2.197222 0 Td 53.596866 Tz (corresponding ) Tj 4.355556 0 Td 45.37037 Tz (to ) Tj 0.758333 0 Td 68.055556 Tz (mate ) Tj 51.428571 0 0 51.428571 0 2373.985879 Tm 25.297222 0 Td 39.699074 Tz (rial ) Tj 1.186111 0 Td 48.611111 Tz (controls ) Tj 2.625 0 Td 62.654321 Tz (F1- ) Tj 1.322222 0 Td 60.493827 Tz (and ) Tj 1.322222 0 Td 63.734568 Tz (F2- ) Tj 1.361111 0 Td 48.611111 Tz (also ) Tj 1.419444 0 Td 61.033951 Tz (showed ) Tj 2.411111 0 Td 49.691358 Tz (the ) Tj 1.108333 0 Td 44.444444 Tz (ability ) Tj 2.119444 0 Td 43.75 Tz (to ) Tj 0.758333 0 Td 43.981481 Tz (attract ) Tj 51.428571 0 0 51.428571 0 2333.896057 Tm 25.297222 0 Td 55.416667 Tz (monocytes, ) Tj 3.422222 0 Td 53.472222 Tz (although ) Tj 2.683333 0 Td 46.990741 Tz (to ) Tj 0.719444 0 Td 48.611111 Tz (the ) Tj 0.991667 0 Td 46.990741 Tz (lesser ) Tj 1.769444 0 Td 46.759259 Tz (extent, ) Tj 2.061111 0 Td 46.990741 Tz (at ) Tj 0.7 0 Td 71.296296 Tz (23% ) Tj 1.438889 0 Td 58.333333 Tz (and ) Tj 1.166667 0 Td 73.45679 Tz (24% ) Tj 1.438889 0 Td 44.560185 Tz (cell ) Tj 51.428571 0 0 51.428571 0 2293.37931 Tm 25.297222 0 Td 49.583333 Tz (migration, ) Tj 3.266667 0 Td 47.364672 Tz (respectively; ) Tj 3.927778 0 Td 51.203704 Tz (these ) Tj 1.827778 0 Td 48.611111 Tz (material ) Tj 2.625 0 Td 48.611111 Tz (control ) Tj 2.333333 0 Td 50.231481 Tz (values ) Tj 2.1 0 Td 58.333333 Tz (were ) Tj 51.428571 0 0 51.428571 0 2250.772671 Tm 25.316667 0 Td 35.648148 Tz (still ) Tj 1.283333 0 Td 51.851852 Tz (a ) Tj 0.544444 0 Td 45.959596 Tz (significant ) Tj 3.266667 0 Td 49.421296 Tz (increase ) Tj 2.586111 0 Td 54.282407 Tz (over ) Tj 1.477778 0 Td 49.691358 Tz (the ) Tj 1.147222 0 Td 92.361111 Tz (DMEM ) Tj 2.469444 0 Td 49.826389 Tz (negative ) Tj 2.605556 0 Td 49.074074 Tz (control ) Tj 51.428571 0 0 51.428571 0 2210.756463 Tm 25.316667 0 Td 52.932099 Tz (\(ps0.03\). ) Tj 3.363889 0 Td 60.277778 Tz (Fewer ) Tj 2.236111 0 Td 57.253086 Tz (monocytes ) Tj 3.5 0 Td 60.493827 Tz (had ) Tj 1.555556 0 Td 53.06713 Tz (migrated ) Tj 2.994444 0 Td 45.37037 Tz (in ) Tj 0.991667 0 Td 48.611111 Tz (the ) Tj 1.322222 0 Td 61.574074 Tz (more ) Tj 51.428571 0 0 51.428571 0 2168.705463 Tm 25.297222 0 Td 60.133745 Tz (demanding ) Tj 3.383333 0 Td 48.611111 Tz (transendothelial ) Tj 4.880556 0 Td 51.131687 Tz (migration ) Tj 2.936111 0 Td 52.5 Tz (assay ) Tj 1.788889 0 Td 58.333333 Tz (where ) Tj 1.925 0 Td 72.376543 Tz (54% ) Tj 1.497222 0 Td 59.41358 Tz (and ) Tj 40 0 0 40 0 2131 Tm 32.55 0 Td 91.666667 Tz (67% ) Tj 1.9 0 Td 75 Tz (of ) Tj 1.025 0 Td 70.138889 Tz (seeded ) Tj 2.725 0 Td 56.666667 Tz (cells ) Tj 1.925 0 Td 76.388889 Tz (had ) Tj 1.6 0 Td 63.425926 Tz (relocated ) Tj 3.65 0 Td 58.333333 Tz (to ) Tj 0.925 0 Td 63.888889 Tz (the ) Tj 1.35 0 Td 71.666667 Tz (lower ) Tj 2.325 0 Td 65.833333 Tz (wells ) Tj 2.225 0 Td 61.111111 Tz (for ) Tj 1.325 0 Td 80.555556 Tz (F1+ ) Tj 51.428571 0 0 51.428571 0 2083.773494 Tm 25.297222 0 Td 58.333333 Tz (and ) Tj 1.225 0 Td 62.654321 Tz (F2+ ) Tj 1.263889 0 Td 48.148148 Tz (eluates ) Tj 2.216667 0 Td 45.868946 Tz (respectively. ) Tj 3.752778 0 Td 49.85755 Tz (Nevertheless, ) Tj 4.083333 0 Td 62.654321 Tz (F1- ) Tj 1.283333 0 Td 58.333333 Tz (and ) Tj 1.205556 0 Td 64.814815 Tz (F2- ) Tj 1.302778 0 Td 62.654321 Tz (elu ) Tj 51.428571 0 0 51.428571 0 2043.56939 Tm 25.297222 0 Td 46.990741 Tz (ates ) Tj 1.438889 0 Td 59.143519 Tz (were ) Tj 1.691667 0 Td 51.041667 Tz (able ) Tj 1.497222 0 Td 48.611111 Tz (to ) Tj 0.913889 0 Td 53.472222 Tz (induce ) Tj 2.216667 0 Td 46.118234 Tz (significantly ) Tj 3.927778 0 Td 61.574074 Tz (more ) Tj 1.808333 0 Td 57.253086 Tz (monocytes ) Tj 3.402778 0 Td 45.37037 Tz (to ) Tj 51.428571 0 0 51.428571 0 2001.727491 Tm 25.277778 0 Td 49.259259 Tz (transverse ) Tj 3.130556 0 Td 49.691358 Tz (the ) Tj 1.069444 0 Td 82.962963 Tz (HUVEC ) Tj 2.683333 0 Td 57.973251 Tz (monolayer ) Tj 3.286111 0 Td 50.231481 Tz (than ) Tj 1.419444 0 Td 48.611111 Tz (the ) Tj 1.05 0 Td 93.981481 Tz (DMEM ) Tj 2.469444 0 Td 49.826389 Tz (negative ) Tj 51.428571 0 0 51.428571 0 1961.720848 Tm 25.297222 0 Td 49.074074 Tz (control ) Tj 2.236111 0 Td 54.12037 Tz (\(ps0.004\). ) Tj 3.422222 0 Td 50 Tz (Unfortunately, ) Tj 4.394444 0 Td 48.611111 Tz (the ) Tj 1.011111 0 Td 73.726852 Tz (fMLP ) Tj 1.886111 0 Td 47.800926 Tz (positive ) Tj 2.45 0 Td 49.074074 Tz (control ) Tj 51.428571 0 0 51.428571 0 1916.530167 Tm 25.297222 0 Td 50.771605 Tz (did ) Tj 1.341667 0 Td 50.771605 Tz (not ) Tj 1.302778 0 Td 53.324916 Tz (demonstrate ) Tj 3.908333 0 Td 51.635802 Tz (chemoattractant ) Tj 5.016667 0 Td 48.287037 Tz (properties ) Tj 3.286111 0 Td 46.990741 Tz (in ) Tj 0.952778 0 Td 46.990741 Tz (either ) Tj 57.142857 0 0 57.142857 0 1874.777911 Tm 22.7675 0 Td 47.25 Tz (assay ) Tj 1.54 0 Td 39.375 Tz (at ) Tj 0.595 0 Td 43.75 Tz (the ) Tj 0.9275 0 Td 51.527778 Tz (low ) Tj 1.0675 0 Td 45.769231 Tz (concentration ) Tj 3.6925 0 Td 42.291667 Tz (tested ) Tj 1.645 0 Td 77 Tz (\(10M\) ) Tj 2.415 0 Td 53.472222 Tz (and ) Tj 1.0675 0 Td 55.416667 Tz (was ) Tj 1.155 0 Td 53.666667 Tz (undis ) Tj 51.428571 0 0 51.428571 0 1836.354557 Tm 25.277778 0 Td 49.421296 Tz (tinguishable ) Tj 3.85 0 Td 57.523148 Tz (from ) Tj 1.652778 0 Td 49.691358 Tz (the ) Tj 1.186111 0 Td 92.361111 Tz (DMEM ) Tj 2.508333 0 Td 49.826389 Tz (negative ) Tj 2.625 0 Td 49.537037 Tz (control ) Tj 2.352778 0 Td 43.75 Tz (in ) Tj 0.777778 0 Td 52.662037 Tz (post-hoc ) Tj 40 0 0 40 0 1799 Tm 32.5 0 Td 56.25 Tz (test ) Tj 1.575 0 Td 54.6875 Tz (results. ) Tj 51.428571 0 0 51.428571 0 1750.305106 Tm 25.997222 0 Td 77.777778 Tz (Now ) Tj 1.555556 0 Td 47.170782 Tz (referring ) Tj 2.625 0 Td 45.37037 Tz (to ) Tj 0.7 0 Td 57.037037 Tz (FIGS. ) Tj 1.847222 0 Td 74.537037 Tz (7A ) Tj 0.991667 0 Td 90.740741 Tz (& ) Tj 0.661111 0 Td 58.333333 Tz (7B, ) Tj 1.147222 0 Td 60.277778 Tz (which ) Tj 1.886111 0 Td 60.277778 Tz (shows ) Tj 1.944444 0 Td 44.444444 Tz (results ) Tj 1.983333 0 Td 59.953704 Tz (from ) Tj 51.428571 0 0 51.428571 0 1711.143219 Tm 25.277778 0 Td 50.771605 Tz (the ) Tj 1.225 0 Td 82.962963 Tz (HUVEC ) Tj 2.819444 0 Td 47.395833 Tz (motility ) Tj 2.566667 0 Td 52.391975 Tz (assays ) Tj 2.197222 0 Td 46.180556 Tz (that ) Tj 1.4 0 Td 54.552469 Tz (demonstrated ) Tj 4.238889 0 Td 48.611111 Tz (the ) Tj 1.166667 0 Td 62.222222 Tz (angio ) Tj 51.428571 0 0 51.428571 0 1670.285369 Tm 25.297222 0 Td 49.109687 Tz (genic-related ) Tj 4.083333 0 Td 51.851852 Tz (chemoattractant ) Tj 4.958333 0 Td 45.37037 Tz (qualities ) Tj 2.741667 0 Td 53.472222 Tz (with ) Tj 1.575 0 Td 50.555556 Tz (these ) Tj 1.788889 0 Td 55.555556 Tz (primary ) Tj 52.857143 0 0 52.857143 0 1625.020833 Tm 24.613514 0 Td 42.882883 Tz (cells ) Tj 1.627027 0 Td 48.348348 Tz (for ) Tj 1.191892 0 Td 52.027027 Tz (both ) Tj 1.589189 0 Td 50.975976 Tz (experimental ) Tj 4.02973 0 Td 58.858859 Tz (and ) Tj 1.381081 0 Td 47.747748 Tz (control ) Tj 2.402703 0 Td 47.297297 Tz (material ) Tj 2.62973 0 Td 44.144144 Tz (eluates. ) Tj 51.428571 0 0 51.428571 0 1584.8 Tm 25.316667 0 Td 57.037037 Tz (FIGS. ) Tj 2.061111 0 Td 72.916667 Tz (7A ) Tj 1.166667 0 Td 90.740741 Tz (& ) Tj 0.894444 0 Td 68.055556 Tz (7B ) Tj 1.147222 0 Td 61.574074 Tz (show ) Tj 1.788889 0 Td 50.411523 Tz (angiogenic-related ) Tj 5.716667 0 Td 52.067901 Tz (chemoattractant ) Tj 51.428571 0 0 51.428571 0 1542.995305 Tm 25.297222 0 Td 45.37037 Tz (qualities ) Tj 2.586111 0 Td 57.523148 Tz (from ) Tj 1.497222 0 Td 55.092593 Tz (both ) Tj 1.458333 0 Td 52.121914 Tz (experimental ) Tj 3.869444 0 Td 59.41358 Tz (and ) Tj 1.186111 0 Td 48.611111 Tz (control ) Tj 2.177778 0 Td 49.421296 Tz (material ) Tj 2.488889 0 Td 45.37037 Tz (eluates. ) Tj 51.428571 0 0 51.428571 0 1500.359721 Tm 25.316667 0 Td 70 Tz (Human ) Tj 2.255556 0 Td 50.05144 Tz (umbilical ) Tj 2.877778 0 Td 50.231481 Tz (vein ) Tj 1.283333 0 Td 49.200337 Tz (endothelial ) Tj 3.344444 0 Td 44.722222 Tz (cells ) Tj 1.497222 0 Td 68.055556 Tz (\(HUVECs\) ) Tj 3.383333 0 Td 52.572016 Tz (migration ) Tj 51.428571 0 0 51.428571 0 1459.073684 Tm 25.297222 0 Td 51.311728 Tz (assays ) Tj 2.158333 0 Td 44.444444 Tz (results ) Tj 2.158333 0 Td 49.691358 Tz (are ) Tj 1.166667 0 Td 56.712963 Tz (shown. ) Tj 2.352778 0 Td 55.092593 Tz (F1+, ) Tj 1.652778 0 Td 54.282407 Tz (F2+, ) Tj 1.633333 0 Td 54.282407 Tz (F1-, ) Tj 1.575 0 Td 59.41358 Tz (and ) Tj 1.361111 0 Td 63.734568 Tz (F2- ) Tj 1.4 0 Td 55.092593 Tz (groups ) Tj 40 0 0 40 0 1423 Tm 32.525 0 Td 61.979167 Tz (indicate ) Tj 3.425 0 Td 63.888889 Tz (the ) Tj 1.625 0 Td 69.444444 Tz (use ) Tj 1.75 0 Td 70.833333 Tz (of ) Tj 1.275 0 Td 62.5 Tz (material ) Tj 3.475 0 Td 58.333333 Tz (eluates. ) Tj 3.3 0 Td 94.791667 Tz (M200 ) Tj 2.75 0 Td 83.333333 Tz (medium ) Tj 3.475 0 Td 77.777778 Tz (and ) Tj 51.428571 0 0 51.428571 0 1376.45283 Tm 25.277778 0 Td 55.632716 Tz (bovine ) Tj 2.158333 0 Td 49.259259 Tz (brain ) Tj 1.652778 0 Td 46.759259 Tz (extract ) Tj 2.119444 0 Td 52.662037 Tz (kit-supplemented ) Tj 5.230556 0 Td 72.106481 Tz (M200 ) Tj 1.905556 0 Td 65.354938 Tz (medium ) Tj 2.527778 0 Td 52.932099 Tz (served ) Tj 51.428571 0 0 51.428571 0 1337.160648 Tm 25.297222 0 Td 50.231481 Tz (as ) Tj 0.894444 0 Td 49.421296 Tz (negative ) Tj 2.605556 0 Td 59.41358 Tz (and ) Tj 1.302778 0 Td 47.395833 Tz (positive ) Tj 2.508333 0 Td 46.450617 Tz (controls, ) Tj 2.8 0 Td 45.868946 Tz (respectively. ) Tj 3.869444 0 Td 56.712963 Tz (Both ) Tj 1.633333 0 Td 51.311728 Tz (assays ) Tj 51.428571 0 0 51.428571 0 1293.994041 Tm 25.297222 0 Td 53.619529 Tz (demonstrate ) Tj 3.752778 0 Td 49.691358 Tz (the ) Tj 1.108333 0 Td 49.826389 Tz (inherent ) Tj 2.625 0 Td 49.691358 Tz (nature ) Tj 2.002778 0 Td 56.712963 Tz (of ) Tj 0.777778 0 Td 50.771605 Tz (the ) Tj 1.108333 0 Td 48.45679 Tz (alginate/B-tricalcium ) Tj 51.428571 0 0 51.428571 0 1250.486553 Tm 25.297222 0 Td 54.73251 Tz (phosphate ) Tj 3.188889 0 Td 56.17284 Tz (raw ) Tj 1.283333 0 Td 48.251029 Tz (materials ) Tj 2.838889 0 Td 42.12963 Tz (to ) Tj 0.758333 0 Td 53.472222 Tz (induce ) Tj 2.119444 0 Td 64.814815 Tz (some ) Tj 1.75 0 Td 82.962963 Tz (HUVEC ) Tj 2.683333 0 Td 47.638889 Tz (activation ) Tj 51.428571 0 0 51.428571 0 1209.85974 Tm 25.316667 0 Td 58.333333 Tz (\(F1- ) Tj 1.652778 0 Td 59.41358 Tz (and ) Tj 1.380556 0 Td 57.523148 Tz (F2-\) ) Tj 1.633333 0 Td 52.662037 Tz (with ) Tj 1.575 0 Td 57.118056 Tz (enhanced ) Tj 3.013889 0 Td 43.981481 Tz (ability ) Tj 2.119444 0 Td 54.444444 Tz (being ) Tj 1.905556 0 Td 62.222222 Tz (shown ) Tj 2.158333 0 Td 48.611111 Tz (for ) Tj 1.108333 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 1168.870159 Tm 25.297222 0 Td 50.854701 Tz (experimental, ) Tj 4.063889 0 Td 67.407407 Tz (human ) Tj 2.119444 0 Td 51.851852 Tz (concentrated ) Tj 3.85 0 Td 44.965278 Tz (platelet ) Tj 2.236111 0 Td 50.898693 Tz (releasate-contain ) Tj 51.428571 0 0 51.428571 0 1126.705596 Tm 25.297222 0 Td 50.771605 Tz (ing ) Tj 1.108333 0 Td 48.251029 Tz (materials ) Tj 2.741667 0 Td 54.444444 Tz (being ) Tj 1.769444 0 Td 50.462963 Tz (evident ) Tj 2.275 0 Td 48.611111 Tz (for ) Tj 0.991667 0 Td 63.734568 Tz (F2+ ) Tj 1.283333 0 Td 53.472222 Tz (only ) Tj 1.458333 0 Td 43.75 Tz (in ) Tj 0.661111 0 Td 50.771605 Tz (the ) Tj 1.05 0 Td 49.826389 Tz (invasion ) Tj 2.547222 0 Td 52.5 Tz (assay ) Tj 51.428571 0 0 51.428571 0 1085.111524 Tm 25.316667 0 Td 51.851852 Tz (\(FIG. ) Tj 1.808333 0 Td 59.41358 Tz (7B\) ) Tj 1.263889 0 Td 59.41358 Tz (and ) Tj 1.283333 0 Td 62.654321 Tz (F1+ ) Tj 1.380556 0 Td 53.098291 Tz (demonstrating ) Tj 4.336111 0 Td 43.75 Tz (this ) Tj 1.263889 0 Td 58.333333 Tz (enhancement ) Tj 4.063889 0 Td 42.12963 Tz (in ) Tj 0.738889 0 Td 55.092593 Tz (both ) Tj 38.571429 0 0 38.571429 0 1047 Tm 33.72963 0 Td 64.197531 Tz (assayS. ) Tj 51.428571 0 0 51.428571 0 1004.039604 Tm 26.036111 0 Td 54.282407 Tz (Experimental ) Tj 4.044444 0 Td 56.712963 Tz (F1 ) Tj 0.777778 0 Td 61.574074 Tz (+ ) Tj 0.544444 0 Td 48.611111 Tz (material ) Tj 2.45 0 Td 49.151235 Tz (eluate ) Tj 1.866667 0 Td 46.759259 Tz (clearly ) Tj 2.1 0 Td 55.092593 Tz (induced ) Tj 2.430556 0 Td 51.851852 Tz (a ) Tj 0.447222 0 Td 49.074074 Tz (signifi ) Tj 51.428571 0 0 51.428571 0 960.456929 Tm 25.297222 0 Td 51.041667 Tz (cant ) Tj 1.438889 0 Td 49.421296 Tz (increase ) Tj 2.605556 0 Td 43.75 Tz (in ) Tj 0.777778 0 Td 82.962963 Tz (HUVEC ) Tj 2.722222 0 Td 51.851852 Tz (migration ) Tj 2.994444 0 Td 55.092593 Tz (over ) Tj 1.516667 0 Td 39.969136 Tz (all ) Tj 0.913889 0 Td 51.851852 Tz (other ) Tj 1.711111 0 Td 55.092593 Tz (groups ) Tj 2.216667 0 Td 48.611111 Tz (in ) Tj 51.428571 0 0 51.428571 0 923.15 Tm 25.277778 0 Td 50.771605 Tz (the ) Tj 1.205556 0 Td 54.552469 Tz (simple ) Tj 2.294444 0 Td 51.49177 Tz (migration ) Tj 3.072222 0 Td 52.5 Tz (assay ) Tj 1.925 0 Td 54.503367 Tz (\(ps0.0001\). ) Tj 3.927778 0 Td 55.902778 Tz (Whereas, ) Tj 3.013889 0 Td 56.712963 Tz (experi ) Tj 51.428571 0 0 51.428571 0 877.946365 Tm 25.297222 0 Td 54.012346 Tz (mental ) Tj 2.138889 0 Td 63.734568 Tz (F2+ ) Tj 1.341667 0 Td 48.611111 Tz (material ) Tj 2.469444 0 Td 48.611111 Tz (eluate ) Tj 1.866667 0 Td 60.493827 Tz (and ) Tj 1.225 0 Td 46.990741 Tz (positive ) Tj 2.411111 0 Td 48.611111 Tz (control ) Tj 2.255556 0 Td 62.546296 Tz (BBE-supple ) Tj 51.428571 0 0 51.428571 0 836.829891 Tm 25.297222 0 Td 59.41358 Tz (mented ) Tj 2.391667 0 Td 72.106481 Tz (M200 ) Tj 1.963889 0 Td 50.771605 Tz (values ) Tj 2.061111 0 Td 53.472222 Tz (only ) Tj 1.536111 0 Td 55.740741 Tz (approached ) Tj 3.597222 0 Td 45.959596 Tz (significant ) Tj 3.266667 0 Td 48.971193 Tz (increases ) Tj 51.428571 0 0 51.428571 0 795.564456 Tm 25.297222 0 Td 54.282407 Tz (over ) Tj 1.497222 0 Td 48.611111 Tz (the ) Tj 1.108333 0 Td 49.421296 Tz (negative ) Tj 2.566667 0 Td 49.074074 Tz (control ) Tj 2.294444 0 Td 72.916667 Tz (M200 ) Tj 1.944444 0 Td 43.75 Tz (in ) Tj 0.777778 0 Td 42.12963 Tz (this ) Tj 1.225 0 Td 51.851852 Tz (assay ) Tj 1.788889 0 Td 55.452675 Tz (\(p=0.0616 ) Tj 3.188889 0 Td 60.493827 Tz (and ) Tj 51.428571 0 0 51.428571 0 754.510968 Tm 25.297222 0 Td 51.851852 Tz (0.0672, ) Tj 2.488889 0 Td 45.833333 Tz (respectively\). ) Tj 4.122222 0 Td 57.118056 Tz (Although ) Tj 3.033333 0 Td 45.37037 Tz (to ) Tj 0.855556 0 Td 51.851852 Tz (a ) Tj 0.622222 0 Td 45.910494 Tz (lesser ) Tj 1.925 0 Td 52.932099 Tz (degree ) Tj 2.216667 0 Td 51.041667 Tz (than ) Tj 1.536111 0 Td 36.728395 Tz (its ) Tj 51.428571 0 0 51.428571 0 714.98568 Tm 25.277778 0 Td 56.388889 Tz (hCPR-containing ) Tj 5.288889 0 Td 52.391975 Tz (experimental ) Tj 3.986111 0 Td 50.673401 Tz (counterpart ) Tj 3.538889 0 Td 45.833333 Tz (eluate, ) Tj 2.177778 0 Td 55.092593 Tz (F1 ) Tj 0.758333 0 Td 64.814815 Tz (- ) Tj 0.602778 0 Td 62.654321 Tz (elu ) Tj 51.428571 0 0 51.428571 0 669.444444 Tm 25.297222 0 Td 47.530864 Tz (ate ) Tj 1.069444 0 Td 61.574074 Tz (was ) Tj 1.341667 0 Td 51.041667 Tz (able ) Tj 1.458333 0 Td 43.75 Tz (to ) Tj 0.777778 0 Td 54.62963 Tz (induced ) Tj 2.527778 0 Td 53.472222 Tz (simple ) Tj 2.177778 0 Td 82.314815 Tz (HUVEC ) Tj 2.722222 0 Td 51.49177 Tz (migration ) Tj 3.013889 0 Td 55.092593 Tz (over ) Tj 1.477778 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 630.4689 Tm 25.297222 0 Td 73.726852 Tz (M200 ) Tj 1.983333 0 Td 49.826389 Tz (negative ) Tj 2.566667 0 Td 49.537037 Tz (control ) Tj 2.313889 0 Td 51.262626 Tz (\(p=0.0455\). ) Tj 3.655556 0 Td 50.084175 Tz (Haptotactic ) Tj 3.5 0 Td 43.75 Tz (activities ) Tj 2.838889 0 Td 58.333333 Tz (of ) Tj 51.428571 0 0 51.428571 0 584.819216 Tm 25.316667 0 Td 76.157407 Tz (HUVECs ) Tj 3.013889 0 Td 43.75 Tz (in ) Tj 0.777778 0 Td 47.530864 Tz (the ) Tj 1.108333 0 Td 49.826389 Tz (invasion ) Tj 2.605556 0 Td 52.5 Tz (assay ) Tj 1.827778 0 Td 58.333333 Tz (were ) Tj 1.652778 0 Td 64.814815 Tz (much ) Tj 1.808333 0 Td 62.384259 Tz (more ) Tj 1.691667 0 Td 50.925926 Tz (evident ) Tj 2.352778 0 Td 58.333333 Tz (of ) Tj 51.428571 0 0 51.428571 0 544.71 Tm 25.297222 0 Td 45.37037 Tz (effects ) Tj 2.119444 0 Td 59.41358 Tz (and ) Tj 1.283333 0 Td 46.720679 Tz (differences. ) Tj 3.577778 0 Td 62.654321 Tz (The ) Tj 1.322222 0 Td 43.981481 Tz (ability ) Tj 2.080556 0 Td 45.37037 Tz (to ) Tj 0.777778 0 Td 54.012346 Tz (induce ) Tj 2.119444 0 Td 55.092593 Tz (a ) Tj 0.563889 0 Td 50.231481 Tz (strong ) Tj 2.002778 0 Td 67.24537 Tz (enzy ) Tj 51.428571 0 0 51.428571 0 502.265207 Tm 25.297222 0 Td 51.041667 Tz (matic-driven ) Tj 3.888889 0 Td 49.826389 Tz (invasion ) Tj 2.625 0 Td 56.712963 Tz (from ) Tj 1.613889 0 Td 76.157407 Tz (HUVECs ) Tj 2.975 0 Td 60.493827 Tz (was ) Tj 1.283333 0 Td 52.662037 Tz (apparent ) Tj 2.722222 0 Td 53.472222 Tz 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Tj 3.791667 0 Td 62.654321 Tz (F1- ) Tj 1.244444 0 Td 60.493827 Tz (and ) Tj 1.244444 0 Td 63.734568 Tz (F2- ) Tj 1.283333 0 Td 55.812757 Tz (\(p=0.0024 ) Tj 3.15 0 Td 60.493827 Tz (and ) Tj 1.225 0 Td 51.388889 Tz (0.0033, ) Tj 2.352778 0 Td 57.253086 Tz (respec ) Tj 51.428571 0 0 51.428571 0 215.923984 Tm 25.277778 0 Td 42.534722 Tz (tively\). ) Tj 2.488889 0 Td 48.611111 Tz (Additionally, ) Tj 4.277778 0 Td 63.734568 Tz (F2+ ) Tj 1.575 0 Td 49.691358 Tz (eluate ) Tj 2.236111 0 Td 52.932099 Tz (Suggested ) Tj 3.286111 0 Td 50.771605 Tz (the ) Tj 1.341667 0 Td 47.800926 Tz (greatest ) Tj 51.428571 0 0 51.428571 0 173.418139 Tm 25.297222 0 Td 50.084175 Tz (chemoattractantability ) Tj 6.727778 0 Td 49.691358 Tz (for ) Tj 0.972222 0 Td 49.907407 Tz (these ) Tj 1.613889 0 Td 41.589506 Tz (cells, ) Tj 1.613889 0 Td 54.444444 Tz (being ) Tj 1.75 0 Td 49.691358 Tz (the ) Tj 1.011111 0 Td 52.662037 Tz (only ) Tj 1.4 0 Td 55.740741 Tz (group ) Tj 1.808333 0 Td 45.37037 Tz (to ) Tj ET Q 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43.75 Tz (cell ) Tj 1.166667 0 Td 54.6875 Tz (coverage ) Tj 2.761111 0 Td 49.421296 Tz (area ) Tj 1.341667 0 Td 54.12037 Tz (\(p=0.0038\) ) Tj 51.428571 0 0 51.428571 0 2829.195954 Tm 6.338889 0 Td 59.62963 Tz (which ) Tj 2.041667 0 Td 49.421296 Tz (also ) Tj 1.477778 0 Td 54.282407 Tz (corresponded ) Tj 4.161111 0 Td 46.990741 Tz (to ) Tj 0.836111 0 Td 56.712963 Tz (an ) Tj 0.952778 0 Td 53.240741 Tz (average ) Tj 2.488889 0 Td 73.45679 Tz (22% ) Tj 1.613889 0 Td 49.826389 Tz (increase ) Tj 2.644444 0 Td 54.282407 Tz (over ) Tj 40 0 0 40 0 2790 Tm 8.2 0 Td 81.944444 Tz (F1+ ) Tj 1.725 0 Td 61.805556 Tz (eluate ) Tj 2.475 0 Td 60.714286 Tz (values. ) Tj 51.428571 0 0 51.428571 0 2742.731844 Tm 7.116667 0 Td 50.771605 Tz (Referring ) Tj 3.091667 0 Td 46.990741 Tz (to ) Tj 0.933333 0 Td 54.282407 Tz (FIG. ) Tj 1.633333 0 Td 46.990741 Tz (8, ) Tj 0.913889 0 Td 45.37037 Tz (in ) Tj 0.933333 0 Td 44.074074 Tz (vitro ) Tj 1.672222 0 Td 52.121914 Tz (angiogenesis ) Tj 4.083333 0 Td 62.654321 Tz (was ) Tj 1.477778 0 Td 47.685185 Tz (further ) Tj 51.428571 0 0 51.428571 0 2700.877301 Tm 6.358333 0 Td 51.446759 Tz (assessed ) Tj 2.683333 0 Td 53.703704 Tz (through ) Tj 2.45 0 Td 50.771605 Tz (the ) Tj 1.108333 0 Td 44.444444 Tz (ability ) Tj 2.080556 0 Td 59.41358 Tz (and ) Tj 1.283333 0 Td 49.151235 Tz (extent ) Tj 2.002778 0 Td 55.092593 Tz (of ) Tj 0.816667 0 Td 53.914141 Tz (spontaneous ) Tj 3.772222 0 Td 53.472222 Tz (tube ) Tj 51.428571 0 0 51.428571 0 2659.910152 Tm 6.358333 0 Td 52.932099 Tz (formation ) Tj 2.975 0 Td 59.953704 Tz (by ) Tj 0.894444 0 Td 69.444444 Tz (HUVECs. ) Tj 3.052778 0 Td 46.296296 Tz (Qualitatively, ) Tj 4.063889 0 Td 54.208754 Tz (micrographs ) Tj 3.694444 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 56.712963 Tz (experi ) Tj 51.428571 0 0 51.428571 0 2615.421995 Tm 6.358333 0 Td 55.092593 Tz (mental ) Tj 2.275 0 Td 49.016204 Tz (material ) Tj 2.586111 0 Td 48.611111 Tz (eluates ) Tj 2.313889 0 Td 57.523148 Tz (from ) Tj 1.711111 0 Td 61.574074 Tz (F1+ ) Tj 1.361111 0 Td 58.333333 Tz (and ) Tj 1.361111 0 Td 61.574074 Tz (F2+ ) Tj 1.4 0 Td 56.30787 Tz (produced ) Tj 2.994444 0 Td 61.574074 Tz (more ) Tj 51.428571 0 0 51.428571 0 2575.704572 Tm 6.338889 0 Td 56.018519 Tz (uniform ) Tj 2.488889 0 Td 42.939815 Tz (cell ) Tj 1.205556 0 Td 49.96142 Tz (organization ) Tj 3.772222 0 Td 51.49177 Tz (networks. ) Tj 2.916667 0 Td 52.350427 Tz (Organizations ) Tj 4.219444 0 Td 51.311728 Tz (within ) Tj 2.002778 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 2534.138298 Tm 6.358333 0 Td 52.391975 Tz (experimental ) Tj 4.491667 0 Td 54.552469 Tz (groups ) Tj 2.683333 0 Td 54.282407 Tz (demonstrated ) Tj 4.588889 0 Td 50.231481 Tz (wider, ) Tj 2.527778 0 Td 52.824074 Tz (continuous ) Tj 51.428571 0 0 51.428571 0 2492.906748 Tm 6.338889 0 Td 51.49177 Tz (branches, ) Tj 2.994444 0 Td 58.333333 Tz (and ) Tj 1.263889 0 Td 44.560185 Tz (less ) Tj 1.302778 0 Td 42.939815 Tz (cell ) Tj 1.225 0 Td 56.388889 Tz (compaction ) Tj 3.577778 0 Td 46.990741 Tz (at ) Tj 0.719444 0 Td 50.231481 Tz (junction ) Tj 2.625 0 Td 46.296296 Tz (points, ) Tj 2.177778 0 Td 53.796296 Tz (while ) Tj 51.428571 0 0 51.428571 0 2450.11 Tm 6.358333 0 Td 51.203704 Tz (other ) Tj 1.808333 0 Td 56.17284 Tz (groups ) Tj 2.352778 0 Td 54.552469 Tz (demonstrated ) Tj 4.277778 0 Td 50.925926 Tz (Smaller ) Tj 2.45 0 Td 53.877315 Tz (branches ) Tj 2.936111 0 Td 52.662037 Tz (with ) Tj 1.594444 0 Td 49.537037 Tz (greater ) Tj 51.428571 0 0 51.428571 0 2408.625298 Tm 6.338889 0 Td 55.092593 Tz (width ) Tj 1.866667 0 Td 44.486532 Tz (variability ) Tj 3.111111 0 Td 59.41358 Tz (and ) Tj 1.263889 0 Td 50.231481 Tz (multiple ) Tj 2.547222 0 Td 53.472222 Tz (branches ) Tj 2.741667 0 Td 58.981481 Tz (which ) Tj 1.963889 0 Td 51.851852 Tz (terminated ) Tj 3.247222 0 Td 64.814815 Tz (on ) Tj 51.428571 0 0 51.428571 0 2367.907655 Tm 6.338889 0 Td 50.771605 Tz (the ) Tj 1.127778 0 Td 47.530864 Tz (Substrate ) Tj 2.741667 0 Td 50.231481 Tz (as ) Tj 0.816667 0 Td 57.87037 Tz (opposed ) Tj 2.586111 0 Td 48.611111 Tz (to ) Tj 0.797222 0 Td 48.935185 Tz (continuity ) Tj 3.111111 0 Td 57.87037 Tz (between ) Tj 2.625 0 Td 60.493827 Tz (two ) Tj 1.263889 0 Td 51.041667 Tz (junction ) Tj 51.428571 0 0 51.428571 0 2326.216389 Tm 6.358333 0 Td 46.296296 Tz (points. ) Tj 2.275 0 Td 86.419753 Tz (MANOVA ) Tj 3.383333 0 Td 59.953704 Tz (of ) Tj 0.933333 0 Td 59.62963 Tz (image ) Tj 2.041667 0 Td 49.421296 Tz (analysis ) Tj 2.683333 0 Td 44.444444 Tz (results ) Tj 2.197222 0 Td 47.530864 Tz (for ) Tj 1.108333 0 Td 42.12963 Tz (total ) Tj 1.594444 0 Td 52.662037 Tz (tube ) Tj 51.428571 0 0 51.428571 0 2283.205562 Tm 6.358333 0 Td 47.685185 Tz (length, ) Tj 2.158333 0 Td 51.851852 Tz (tube ) Tj 1.4 0 Td 49.691358 Tz (count, ) Tj 1.925 0 Td 59.41358 Tz (and ) Tj 1.166667 0 Td 50.636574 Tz (junction ) Tj 2.605556 0 Td 53.148148 Tz (count ) Tj 1.75 0 Td 54.282407 Tz (demonstrated ) Tj 4.063889 0 Td 55.092593 Tz (a ) Tj 0.466667 0 Td 57.037037 Tz (Wilks ) Tj 51.428571 0 0 51.428571 0 2243.57385 Tm 6.358333 0 Td 59.259259 Tz (V=0.081 ) Tj 2.780556 0 Td 54.444444 Tz (\(p=0.0098\) ) Tj 3.461111 0 Td 53.472222 Tz (with ) Tj 1.516667 0 Td 49.691358 Tz (the ) Tj 1.088889 0 Td 48.611111 Tz (large ) Tj 1.672222 0 Td 51.041667 Tz (majority ) Tj 2.644444 0 Td 59.953704 Tz (of ) Tj 0.816667 0 Td 53.030303 Tz (accountable ) Tj 51.428571 0 0 51.428571 0 2199.589189 Tm 6.358333 0 Td 50.636574 Tz (variance ) Tj 2.819444 0 Td 50.771605 Tz (within ) Tj 2.275 0 Td 48.611111 Tz (the ) Tj 1.283333 0 Td 58.333333 Tz (model ) Tj 2.177778 0 Td 59.548611 Tz (stemming ) Tj 3.227778 0 Td 57.523148 Tz (from ) Tj 1.788889 0 Td 49.691358 Tz (the ) Tj 1.244444 0 Td 46.450617 Tz (effect ) Tj 2.041667 0 Td 58.333333 Tz (of ) Tj 51.428571 0 0 51.428571 0 2157 Tm 6.358333 0 Td 76.5625 Tz (MATRIGEL ) Tj 3.927778 0 Td 69.135802 Tz (\(BD ) Tj 1.516667 0 Td 50.501543 Tz (Biosciences; ) Tj 3.927778 0 Td 50.636574 Tz (Franklin ) Tj 2.702778 0 Td 51.311728 Tz (Lakes, ) Tj 2.119444 0 Td 47.314815 Tz (N.J.\) ) Tj 1.633333 0 Td 55.740741 Tz (blend ) Tj 51.428571 0 0 51.428571 0 2117.452632 Tm 6.338889 0 Td 53.472222 Tz (type ) Tj 1.555556 0 Td 59.953704 Tz (on ) Tj 1.05 0 Td 41.481481 Tz (total ) Tj 1.594444 0 Td 51.041667 Tz (tube ) Tj 1.536111 0 Td 47.222222 Tz (length; ) Tj 2.275 0 Td 54.444444 Tz (blend ) Tj 1.925 0 Td 52.662037 Tz (type ) Tj 1.555556 0 Td 51.851852 Tz (did ) Tj 1.244444 0 Td 50.771605 Tz (not ) Tj 1.186111 0 Td 54.208754 Tz (demonstrate ) Tj 51.428571 0 0 51.428571 0 2076.007444 Tm 6.377778 0 Td 48.070988 Tz (significance ) Tj 3.713889 0 Td 45.37037 Tz (in ) Tj 0.797222 0 Td 52.662037 Tz (tube ) Tj 1.477778 0 Td 60.493827 Tz (and ) Tj 1.263889 0 Td 50.636574 Tz (junction ) Tj 2.683333 0 Td 54.444444 Tz (count ) Tj 1.905556 0 Td 47.530864 Tz (variance. ) Tj 2.897222 0 Td 55.902778 Tz (However, ) Tj 51.428571 0 0 51.428571 0 2034.876074 Tm 6.358333 0 Td 58.333333 Tz (and ) Tj 1.225 0 Td 50.231481 Tz (as ) Tj 0.797222 0 Td 51.131687 Tz (expected, ) Tj 2.955556 0 Td 58.333333 Tz (from ) Tj 1.613889 0 Td 50.771605 Tz (the ) Tj 1.108333 0 Td 49.691358 Tz (nature ) Tj 1.963889 0 Td 56.712963 Tz (of ) Tj 0.797222 0 Td 55.092593 Tz (Such ) Tj 1.516667 0 Td 54.372428 Tz (branching ) Tj 3.130556 0 Td 56.944444 Tz (network ) Tj 51.428571 0 0 51.428571 0 1994.172099 Tm 6.377778 0 Td 51.446759 Tz (systems, ) Tj 2.780556 0 Td 53.472222 Tz (tube ) Tj 1.594444 0 Td 60.493827 Tz (and ) Tj 1.361111 0 Td 50.636574 Tz (junction ) Tj 2.8 0 Td 53.796296 Tz (count ) Tj 1.944444 0 Td 59.143519 Tz (were ) Tj 1.730556 0 Td 47.962963 Tz (predictive ) Tj 3.247222 0 Td 56.712963 Tz (of ) Tj 0.952778 0 Td 58.333333 Tz (one ) Tj 51.428571 0 0 51.428571 0 1951.269576 Tm 6.358333 0 Td 48.611111 Tz (another, ) Tj 2.780556 0 Td 55.092593 Tz (producing ) Tj 3.402778 0 Td 55.092593 Tz (high ) Tj 1.788889 0 Td 51.131687 Tz (canonical ) Tj 3.227778 0 Td 47.43266 Tz (correlation ) Tj 3.616667 0 Td 48.611111 Tz (\(0.9726\). ) Tj 51.428571 0 0 51.428571 0 1909.589474 Tm 6.377778 0 Td 49.583333 Tz (Reflecting ) Tj 3.266667 0 Td 58.333333 Tz (back ) Tj 1.672222 0 Td 56.712963 Tz (on ) Tj 1.011111 0 Td 50.231481 Tz (length ) Tj 2.138889 0 Td 52.932099 Tz (measures, ) Tj 3.169444 0 Td 63.734568 Tz (F1+ ) Tj 1.458333 0 Td 45.37037 Tz (eluate, ) Tj 2.177778 0 Td 60.493827 Tz (and ) Tj 1.341667 0 Td 48.611111 Tz (to ) Tj 0.894444 0 Td 55.092593 Tz (a ) Tj 51.428571 0 0 51.428571 0 1870.673317 Tm 6.358333 0 Td 46.990741 Tz (lesser ) Tj 1.944444 0 Td 49.537037 Tz (degree, ) Tj 2.391667 0 Td 62.654321 Tz (F2+ ) Tj 1.380556 0 Td 49.691358 Tz (eluate ) Tj 2.022222 0 Td 54.552469 Tz (demonstrated ) Tj 4.2 0 Td 47.43266 Tz (Superiority ) Tj 3.422222 0 Td 45.37037 Tz (in ) Tj 0.797222 0 Td 43.425926 Tz (total ) Tj 51.428571 0 0 51.428571 0 1830.49441 Tm 6.358333 0 Td 50.231481 Tz (length ) Tj 2.158333 0 Td 55.497685 Tz (measures ) Tj 2.955556 0 Td 54.282407 Tz (over ) Tj 1.575 0 Td 45.37037 Tz (their ) Tj 1.652778 0 Td 63.599537 Tz (non-hCPR ) Tj 3.344444 0 Td 51.203704 Tz (containing ) Tj 3.383333 0 Td 56.944444 Tz (counter ) Tj 51.428571 0 0 51.428571 0 1791.209246 Tm 6.338889 0 Td 47.314815 Tz (parts ) Tj 1.594444 0 Td 54.282407 Tz (with ) Tj 1.477778 0 Td 70.486111 Tz (255% ) Tj 1.886111 0 Td 58.333333 Tz (and ) Tj 1.186111 0 Td 72.106481 Tz (220% ) Tj 1.925 0 Td 48.251029 Tz (increases ) Tj 2.838889 0 Td 55.452675 Tz (\(p=0.0024 ) Tj 3.15 0 Td 58.333333 Tz (and ) Tj 1.263889 0 Td 50.925926 Tz (0.0192, ) Tj 51.428571 0 0 51.428571 0 1757 Tm 6.358333 0 Td 46.296296 Tz (respectively\). ) Tj 51.428571 0 0 51.428571 0 1710.825157 Tm 7.116667 0 Td 70 Tz (Human ) Tj 2.313889 0 Td 50.411523 Tz (umbilical ) Tj 2.975 0 Td 49.421296 Tz (vein ) Tj 1.380556 0 Td 48.905724 Tz (endothelial ) Tj 3.422222 0 Td 44.722222 Tz (cells ) Tj 1.536111 0 Td 68.460648 Tz (\(HUVECs\) ) Tj 3.480556 0 Td 66.435185 Tz (spon ) Tj 51.428571 0 0 51.428571 0 1672.06135 Tm 6.338889 0 Td 53.703704 Tz (taneous ) Tj 2.469444 0 Td 52.662037 Tz (tube ) Tj 1.516667 0 Td 52.572016 Tz (formation ) Tj 3.052778 0 Td 53.148148 Tz (assay ) Tj 1.827778 0 Td 61.574074 Tz (on ) Tj 0.972222 0 Td 51.851852 Tz (Matrigel ) Tj 2.722222 0 Td 55.812757 Tz (\(basement ) Tj 3.266667 0 Td 91.820988 Tz (mem ) Tj 52.857143 0 0 52.857143 0 1630.593711 Tm 6.167568 0 Td 53.603604 Tz (brane ) Tj 1.778378 0 Td 46.509009 Tz (gel\) ) Tj 1.286486 0 Td 51.501502 Tz (blends ) Tj 2.081081 0 Td 50.45045 Tz (with ) Tj 1.418919 0 Td 41.779279 Tz (test ) Tj 1.191892 0 Td 46.946947 Tz (solutions ) Tj 2.762162 0 Td 58.858859 Tz (was ) Tj 1.267568 0 Td 45.720721 Tz (studied. ) Tj 2.459459 0 Td 51.238739 Tz (Fl+, ) Tj 1.513514 0 Td 51.238739 Tz (F2+, ) Tj 51.428571 0 0 51.428571 0 1591.019108 Tm 6.377778 0 Td 55.092593 Tz (F1-, ) Tj 1.536111 0 Td 59.41358 Tz (and ) Tj 1.302778 0 Td 63.734568 Tz (F2- ) Tj 1.361111 0 Td 54.012346 Tz (groups ) Tj 2.216667 0 Td 47.800926 Tz (indicate ) Tj 2.508333 0 Td 49.691358 Tz (the ) Tj 1.127778 0 Td 52.932099 Tz (use ) Tj 1.166667 0 Td 58.333333 Tz (of ) Tj 0.855556 0 Td 48.611111 Tz (material ) Tj 2.547222 0 Td 48.148148 Tz (eluates ) Tj 2.275 0 Td 48.611111 Tz (in ) Tj 51.428571 0 0 51.428571 0 1552.743462 Tm 6.377778 0 Td 79.722222 Tz (DMEM. ) Tj 2.741667 0 Td 72.106481 Tz (M200 ) Tj 2.022222 0 Td 47.260802 Tz (\(Invitrogen; ) Tj 3.713889 0 Td 51.49177 Tz (Carlsbad, ) Tj 3.072222 0 Td 44.907407 Tz (Calif.\) ) Tj 2.216667 0 Td 64.274691 Tz (medium ) Tj 2.605556 0 Td 61.574074 Tz (and ) Tj 51.428571 0 0 51.428571 0 1513.462985 Tm 6.338889 0 Td 55.632716 Tz (bovine ) Tj 2.158333 0 Td 49.259259 Tz (brain ) Tj 1.652778 0 Td 46.759259 Tz (extract ) Tj 2.119444 0 Td 53.06713 Tz (kit-supplemented ) Tj 5.230556 0 Td 72.106481 Tz (M200 ) Tj 1.905556 0 Td 65.354938 Tz (medium ) Tj 2.527778 0 Td 53.472222 Tz (served ) Tj 51.428571 0 0 51.428571 0 1476.066826 Tm 6.358333 0 Td 50.231481 Tz (as ) Tj 0.816667 0 Td 49.826389 Tz (negative ) Tj 2.527778 0 Td 59.41358 Tz (and ) Tj 1.225 0 Td 47.800926 Tz (positive ) Tj 2.45 0 Td 46.450617 Tz (controls, ) Tj 2.683333 0 Td 46.118234 Tz (respectively. ) Tj 3.772222 0 Td 50.231481 Tz (Test ) Tj 1.361111 0 Td 48.611111 Tz (Solutions ) Tj 51.428571 0 0 51.428571 0 1433.267327 Tm 6.338889 0 Td 59.953704 Tz (were ) Tj 1.613889 0 Td 59.62963 Tz (mixed ) Tj 1.944444 0 Td 51.851852 Tz (with ) Tj 1.419444 0 Td 51.851852 Tz (Matrigel ) Tj 2.644444 0 Td 43.75 Tz (at ) Tj 0.680556 0 Td 46.450617 Tz (2:1 ) Tj 1.108333 0 Td 40.509259 Tz (ratio, ) Tj 1.594444 0 Td 59.41358 Tz (and ) Tj 1.244444 0 Td 58.333333 Tz (65ul ) Tj 1.555556 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 50.771605 Tz (the ) Tj 1.05 0 Td 52.391975 Tz (blends ) Tj 52.857143 0 0 52.857143 0 1393.851897 Tm 6.167568 0 Td 58.333333 Tz (were ) Tj 1.57027 0 Td 52.252252 Tz (allowed ) Tj 2.383784 0 Td 44.144144 Tz (to ) Tj 0.718919 0 Td 51.238739 Tz (cure ) Tj 1.4 0 Td 42.567568 Tz (in ) Tj 0.681081 0 Td 51.801802 Tz (96-well ) Tj 2.345946 0 Td 45.720721 Tz (plates ) Tj 1.816216 0 Td 48.348348 Tz (\(n=3\). ) Tj 1.948649 0 Td 67.477477 Tz (5x104 ) Tj 2.175676 0 Td 55.705706 Tz (second ) Tj 51.428571 0 0 51.428571 0 1354.582524 Tm 6.358333 0 Td 54.166667 Tz (passage ) Tj 2.469444 0 Td 76.157407 Tz (HUVECs ) Tj 2.955556 0 Td 58.333333 Tz (were ) Tj 1.594444 0 Td 54.552469 Tz (seeded ) Tj 2.119444 0 Td 63.194444 Tz (on ) Tj 0.913889 0 Td 49.691358 Tz (gel ) Tj 1.088889 0 Td 46.450617 Tz (surfaces. ) Tj 2.683333 0 Td 62.384259 Tz (Tube ) Tj 1.652778 0 Td 66.111111 Tz (forma ) Tj 51.428571 0 0 51.428571 0 1317.600998 Tm 6.338889 0 Td 47.800926 Tz (tion ) Tj 1.380556 0 Td 61.574074 Tz (was ) Tj 1.322222 0 Td 51.446759 Tz (assessed ) Tj 2.683333 0 Td 53.148148 Tz (using ) Tj 1.808333 0 Td 43.518519 Tz (digital ) Tj 2.041667 0 Td 62.654321 Tz (40x ) Tj 1.380556 0 Td 54.503367 Tz (micrographs ) Tj 3.811111 0 Td 59.953704 Tz (of ) Tj 0.816667 0 Td 49.691358 Tz (the ) Tj 1.127778 0 Td 61.574074 Tz (cul ) Tj 51.428571 0 0 51.428571 0 1276.945813 Tm 6.338889 0 Td 47.314815 Tz (tures ) Tj 1.691667 0 Td 53.472222 Tz (12 ) Tj 0.855556 0 Td 53.796296 Tz (hours ) Tj 1.808333 0 Td 45.37037 Tz (after ) Tj 1.536111 0 Td 49.421296 Tz (seeding. ) Tj 2.605556 0 Td 47.800926 Tz (FIJI/Image.J ) Tj 3.655556 0 Td 51.851852 Tz (software ) Tj 2.683333 0 Td 61.574074 Tz (was ) Tj 1.341667 0 Td 55.902778 Tz (used ) Tj 51.428571 0 0 51.428571 0 1237.033413 Tm 6.338889 0 Td 46.990741 Tz (to ) Tj 0.7 0 Td 49.016204 Tz (analysis ) Tj 2.488889 0 Td 52.662037 Tz (tube ) Tj 1.4 0 Td 50.084175 Tz (formations; ) Tj 3.441667 0 Td 42.777778 Tz (total ) Tj 1.438889 0 Td 52.662037 Tz (tube ) Tj 1.4 0 Td 49.691358 Tz (length ) Tj 1.944444 0 Td 52.5 Tz (\(Lm\), ) Tj 1.75 0 Td 61.574074 Tz (number ) Tj 2.333333 0 Td 56.712963 Tz (of ) Tj 51.428571 0 0 51.428571 0 1197.497462 Tm 6.338889 0 Td 48.070988 Tz (tubes, ) Tj 2.041667 0 Td 59.41358 Tz (and ) Tj 1.419444 0 Td 61.574074 Tz (number ) Tj 2.508333 0 Td 58.333333 Tz (of ) Tj 0.875 0 Td 50.05144 Tz (junctions ) Tj 3.033333 0 Td 59.143519 Tz (were ) Tj 1.75 0 Td 51.557239 Tz (determined. ) Tj 3.772222 0 Td 57.79321 Tz (Images ) Tj 51.428571 0 0 51.428571 0 1157.231504 Tm 6.338889 0 Td 59.953704 Tz (were ) Tj 1.575 0 Td 52.256944 Tz (captured ) Tj 2.605556 0 Td 45.37037 Tz (after ) Tj 1.555556 0 Td 55.092593 Tz (12 ) Tj 0.777778 0 Td 50.231481 Tz (hours. ) Tj 1.963889 0 Td 72.916667 Tz (IMAGEJ ) Tj 2.761111 0 Td 56.388889 Tz (\(NIH: ) Tj 1.886111 0 Td 51.851852 Tz (Bethesda, ) Tj 2.955556 0 Td 46.666667 Tz (N.J.\) ) Tj 51.428571 0 0 51.428571 0 1117.024876 Tm 6.377778 0 Td 51.851852 Tz (software ) Tj 2.722222 0 Td 60.493827 Tz (was ) Tj 1.322222 0 Td 55.902778 Tz (used ) Tj 1.575 0 Td 45.37037 Tz (to ) Tj 0.797222 0 Td 53.652263 Tz (determine ) Tj 3.130556 0 Td 42.12963 Tz (total ) Tj 1.477778 0 Td 55.092593 Tz (branch ) Tj 2.216667 0 Td 47.685185 Tz (length, ) Tj 2.216667 0 Td 56.17284 Tz (branch ) Tj 51.428571 0 0 51.428571 0 1079.342649 Tm 6.358333 0 Td 49.691358 Tz (count, ) Tj 1.963889 0 Td 58.333333 Tz (and ) Tj 1.147222 0 Td 50.636574 Tz (junction ) Tj 2.566667 0 Td 50.231481 Tz (count, ) Tj 2.002778 0 Td 61.574074 Tz (shown ) Tj 2.041667 0 Td 43.75 Tz (in ) Tj 0.738889 0 Td 56.388889 Tz (FIGS. ) Tj 1.847222 0 Td 66.759259 Tz (8A-8C ) Tj 2.158333 0 Td 50.231481 Tz (as ) Tj 0.758333 0 Td 53.703704 Tz (average ) Tj 51.428571 0 0 51.428571 0 1039.755981 Tm 6.358333 0 Td 50.771605 Tz (values ) Tj 2.002778 0 Td 53.472222 Tz (with ) Tj 1.438889 0 Td 50.231481 Tz (bars ) Tj 1.4 0 Td 57.87037 Tz (showing ) Tj 2.586111 0 Td 52.824074 Tz (plus/minus ) Tj 3.363889 0 Td 57.253086 Tz (one ) Tj 1.186111 0 Td 51.446759 Tz (standard ) Tj 2.625 0 Td 47.314815 Tz (deviation. ) Tj 51.428571 0 0 51.428571 0 999.142857 Tm 6.358333 0 Td 49.421296 Tz (Multivariate ) Tj 3.713889 0 Td 49.016204 Tz (analysis ) Tj 2.547222 0 Td 54.12037 Tz (determined ) Tj 3.422222 0 Td 48.611111 Tz (the ) Tj 1.030556 0 Td 51.58179 Tz (eluate/media ) Tj 3.908333 0 Td 42.12963 Tz (test ) Tj 1.166667 0 Td 47.530864 Tz (factor ) Tj 51.428571 0 0 51.428571 0 962.268015 Tm 6.338889 0 Td 45.37037 Tz (to ) Tj 0.777778 0 Td 57.523148 Tz (have ) Tj 1.575 0 Td 55.092593 Tz (a ) Tj 0.583333 0 Td 45.37037 Tz (significant ) Tj 3.208333 0 Td 46.450617 Tz (effect ) Tj 1.866667 0 Td 59.953704 Tz (on ) Tj 0.952778 0 Td 51.851852 Tz (tube ) Tj 1.497222 0 Td 49.691358 Tz (length ) Tj 2.002778 0 Td 51.851852 Tz (\(p=0.0098\). ) Tj 3.713889 0 Td 53.472222 Tz (FIG. ) Tj 51.428571 0 0 51.428571 0 921.048077 Tm 6.358333 0 Td 74.537037 Tz (8A ) Tj 1.030556 0 Td 58.981481 Tz (shows ) Tj 1.905556 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 43.981481 Tz (results ) Tj 1.963889 0 Td 58.333333 Tz (from ) Tj 1.536111 0 Td 49.691358 Tz (the ) Tj 1.011111 0 Td 42.777778 Tz (total ) Tj 1.419444 0 Td 54.552469 Tz (branch ) Tj 2.1 0 Td 50.231481 Tz (length ) Tj 1.944444 0 Td 48.611111 Tz (analysis ) Tj 2.45 0 Td 60.493827 Tz (and ) Tj 51.428571 0 0 51.428571 0 879.879331 Tm 6.358333 0 Td 53.20216 Tz (demonstrates ) Tj 3.986111 0 Td 50.411523 Tz (increased ) Tj 2.877778 0 Td 42.12963 Tz (total ) Tj 1.380556 0 Td 54.552469 Tz (branch ) Tj 2.119444 0 Td 49.691358 Tz (length ) Tj 1.925 0 Td 58.333333 Tz (from ) Tj 1.575 0 Td 62.654321 Tz (F1+ ) Tj 1.263889 0 Td 58.333333 Tz (and ) Tj 1.205556 0 Td 62.654321 Tz (F2+ ) Tj 51.428571 0 0 51.428571 0 843.485437 Tm 6.358333 0 Td 45.910494 Tz (elates ) Tj 1.808333 0 Td 46.990741 Tz (in ) Tj 0.738889 0 Td 56.388889 Tz (comparison ) Tj 3.538889 0 Td 46.990741 Tz (to ) Tj 0.719444 0 Td 46.018519 Tz (their ) Tj 1.497222 0 Td 49.96142 Tz (counterparts ) Tj 3.752778 0 Td 58.981481 Tz (which ) Tj 1.963889 0 Td 50.771605 Tz (did ) Tj 1.088889 0 Td 50.771605 Tz (not ) Tj 1.069444 0 Td 71.296296 Tz (con ) Tj 51.428571 0 0 51.428571 0 802.636704 Tm 6.338889 0 Td 46.180556 Tz (tain ) Tj 1.438889 0 Td 65.462963 Tz (human ) Tj 2.294444 0 Td 51.58179 Tz (concentrated ) Tj 4.044444 0 Td 44.560185 Tz (platelet ) Tj 2.469444 0 Td 47.170782 Tz (releasate ) Tj 2.858333 0 Td 55.452675 Tz (\(p=0.0024 ) Tj 3.305556 0 Td 61.574074 Tz (and ) Tj 51.428571 0 0 51.428571 0 766.126214 Tm 6.358333 0 Td 51.388889 Tz (0.0192, ) Tj 2.391667 0 Td 45.833333 Tz (respectively\). ) Tj 40 0 0 40 0 729 Tm 9.15 0 Td 73.333333 Tz (FIGS. ) Tj 2.475 0 Td 89.583333 Tz (8B ) Tj 1.375 0 Td 116.666667 Tz (& ) Tj 0.975 0 Td 93.75 Tz (8C ) Tj 1.4 0 Td 79.166667 Tz (show ) Tj 2.225 0 Td 63.888889 Tz (the ) Tj 1.4 0 Td 57.738095 Tz (results ) Tj 2.725 0 Td 73.958333 Tz (from ) Tj 2.075 0 Td 55 Tz (total ) Tj 1.95 0 Td 70.138889 Tz (branch ) Tj 2.8 0 Td 70 Tz (count ) Tj 51.428571 0 0 51.428571 0 684.983213 Tm 6.358333 0 Td 58.333333 Tz (and ) Tj 1.205556 0 Td 42.777778 Tz (total ) Tj 1.438889 0 Td 50.231481 Tz (junction ) Tj 2.586111 0 Td 54.444444 Tz (count ) Tj 1.808333 0 Td 52.932099 Tz (measures, ) Tj 3.052778 0 Td 46.616809 Tz (respectively; ) Tj 3.869444 0 Td 48.148148 Tz (neither ) Tj 2.177778 0 Td 55.092593 Tz (vari ) Tj 51.428571 0 0 51.428571 0 642.697789 Tm 6.358333 0 Td 51.041667 Tz (able ) Tj 1.361111 0 Td 60.493827 Tz (was ) Tj 1.263889 0 Td 56.388889 Tz (found ) Tj 1.847222 0 Td 45.37037 Tz (to ) Tj 0.7 0 Td 56.712963 Tz (be ) Tj 0.797222 0 Td 48.611111 Tz (effected ) Tj 2.488889 0 Td 50.231481 Tz (as ) Tj 0.738889 0 Td 51.851852 Tz (a ) Tj 0.505556 0 Td 44.290123 Tz (result ) Tj 1.730556 0 Td 53.472222 Tz (of ) Tj 0.719444 0 Td 51.851852 Tz (eluate/media ) Tj 3.908333 0 Td 46.018519 Tz (type. ) Tj 51.428571 0 0 51.428571 0 605.702179 Tm 6.377778 0 Td 61.111111 Tz (Example ) Tj 2.722222 0 Td 55.092593 Tz (2 ) Tj 0.486111 0 Td 58.333333 Tz (shows ) Tj 1.925 0 Td 49.691358 Tz (the ) Tj 1.011111 0 Td 46.990741 Tz (in ) Tj 0.719444 0 Td 44.722222 Tz (vitro ) Tj 1.497222 0 Td 48.611111 Tz (the ) Tj 1.011111 0 Td 47.638889 Tz (capability ) Tj 2.994444 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 50.771605 Tz (the ) Tj 1.030556 0 Td 50.084175 Tz (biomaterial ) Tj 51.428571 0 0 51.428571 0 568.667072 Tm 6.358333 0 Td 55.452675 Tz (composite ) Tj 3.188889 0 Td 53.796296 Tz (composites ) Tj 3.422222 0 Td 45.37037 Tz (to ) Tj 0.719444 0 Td 54.552469 Tz (induce ) Tj 2.158333 0 Td 46.990741 Tz (motility ) Tj 2.45 0 Td 43.75 Tz (in ) Tj 0.719444 0 Td 53.472222 Tz (both ) Tj 1.438889 0 Td 58.333333 Tz (a ) Tj 0.525 0 Td 59.548611 Tz (monocyte ) Tj 51.428571 0 0 51.428571 0 526.698565 Tm 6.358333 0 Td 43.75 Tz (cell ) Tj 1.225 0 Td 46.180556 Tz (line ) Tj 1.263889 0 Td 58.333333 Tz (and ) Tj 1.244444 0 Td 54.166667 Tz (primary ) Tj 2.469444 0 Td 58.333333 Tz (immature ) Tj 2.955556 0 Td 49.200337 Tz (endothelial ) Tj 3.422222 0 Td 42.12963 Tz (cells, ) Tj 1.711111 0 Td 53.472222 Tz (conjugated ) Tj 51.428571 0 0 51.428571 0 489.677749 Tm 6.358333 0 Td 47.800926 Tz (motility ) Tj 2.663889 0 Td 56.712963 Tz (of ) Tj 0.952778 0 Td 57.613169 Tz (monocytes ) Tj 3.441667 0 Td 54.166667 Tz (through ) Tj 2.605556 0 Td 58.333333 Tz (a ) Tj 0.7 0 Td 50.05144 Tz (confluent ) Tj 3.072222 0 Td 57.613169 Tz (monolayer ) Tj 3.441667 0 Td 56.712963 Tz (of ) Tj 51.428571 0 0 51.428571 0 447.839329 Tm 6.358333 0 Td 48.905724 Tz (endothelial ) Tj 3.538889 0 Td 42.12963 Tz (cells, ) Tj 1.866667 0 Td 58.796296 Tz (modeled ) Tj 2.761111 0 Td 50.487329 Tz (haptotactic-induced ) Tj 6.047222 0 Td 49.200337 Tz (endothelial ) Tj 51.428571 0 0 51.428571 0 407.809235 Tm 6.358333 0 Td 43.75 Tz (cell ) Tj 1.283333 0 Td 54.62963 Tz (passage ) Tj 2.527778 0 Td 54.166667 Tz (through ) Tj 2.547222 0 Td 57.523148 Tz (basement ) Tj 3.013889 0 Td 57.118056 Tz (membrane-derived ) Tj 5.716667 0 Td 43.75 Tz (gel, ) Tj 1.322222 0 Td 60.493827 Tz (and ) Tj 51.428571 0 0 51.428571 0 367.762874 Tm 6.358333 0 Td 54.282407 Tz (observed ) Tj 2.8 0 Td 54.208754 Tz (spontaneous ) Tj 3.772222 0 Td 50.771605 Tz (induction ) Tj 2.916667 0 Td 59.953704 Tz (of ) Tj 0.816667 0 Td 48.611111 Tz (endothelial ) Tj 3.402778 0 Td 43.75 Tz (cell ) Tj 1.244444 0 Td 52.662037 Tz (tube ) Tj 1.438889 0 Td 59.41358 Tz (for ) Tj 51.428571 0 0 51.428571 0 329.577396 Tm 6.358333 0 Td 55.632716 Tz (mation ) Tj 2.216667 0 Td 39.969136 Tz (all ) Tj 0.952778 0 Td 58.333333 Tz (of ) Tj 0.816667 0 Td 58.333333 Tz (which ) Tj 1.963889 0 Td 43.518519 Tz (reflect ) Tj 2.041667 0 Td 56.712963 Tz (on ) Tj 0.933333 0 Td 54.012346 Tz (inflammatory ) Tj 4.083333 0 Td 59.41358 Tz (and ) Tj 1.263889 0 Td 53.148148 Tz (angiogenic ) Tj 51.428571 0 0 51.428571 0 292.471503 Tm 6.358333 0 Td 49.907407 Tz (responses. ) Tj 3.363889 0 Td 48.881173 Tz (Collectively ) Tj 3.888889 0 Td 50.771605 Tz (the ) Tj 1.283333 0 Td 49.074074 Tz (current ) Tj 2.391667 0 Td 60.493827 Tz (and ) Tj 1.438889 0 Td 47.962963 Tz (prior ) Tj 1.808333 0 Td 48.206019 Tz (findings ) Tj 2.702778 0 Td 56.712963 Tz (of ) Tj 51.428571 0 0 51.428571 0 251.604534 Tm 6.358333 0 Td 59.548611 Tz (monocyte ) Tj 3.266667 0 Td 46.990741 Tz (activation ) Tj 3.208333 0 Td 60.493827 Tz (and ) Tj 1.497222 0 Td 52.875244 Tz (osteochondrogenesis ) Tj 6.436111 0 Td 56.712963 Tz (have ) Tj 1.769444 0 Td 71.296296 Tz (con ) Tj 51.428571 0 0 51.428571 0 214.574257 Tm 6.358333 0 Td 52.932099 Tz (firmed ) Tj 2.313889 0 Td 49.691358 Tz (the ) Tj 1.263889 0 Td 56.388889 Tz (hCPR-containing ) Tj 5.483333 0 Td 53.703704 Tz (alginate/B-TCP ) Tj 4.861111 0 Td 55.416667 Tz (composites ) Tj 51.428571 0 0 51.428571 0 170.356021 Tm 6.358333 0 Td 43.981481 Tz (ability ) Tj 2.119444 0 Td 45.37037 Tz (to ) Tj 0.816667 0 Td 53.472222 Tz (induce ) Tj 2.177778 0 Td 55.092593 Tz (an ) Tj 0.952778 0 Td 43.75 Tz (in ) Tj 0.816667 0 Td 44.722222 Tz (vitro ) Tj 1.613889 0 Td 52.662037 Tz (response ) Tj 2.8 0 Td 48.148148 Tz (similar ) Tj 2.236111 0 Td 46.990741 Tz (to ) Tj 0.816667 0 Td 46.180556 Tz (that ) Tj 1.361111 0 Td 59.62963 Tz (which ) Tj 30 0 0 30 0 2755 Tm 41.833333 0 Td 72.222222 Tz (5 ) Tj 30 0 0 30 0 2547 Tm 41.666667 0 Td 75 Tz (10 ) Tj 30 0 0 30 0 2339 Tm 41.666667 0 Td 72.222222 Tz (15 ) Tj 30 0 0 30 0 1923 Tm 41.6 0 Td 77.777778 Tz (25 ) Tj 30 0 0 30 0 1716 Tm 41.6 0 Td 80.555556 Tz (30 ) Tj 30 0 0 30 0 1507 Tm 41.6 0 Td 77.777778 Tz (35 ) Tj 30 0 0 30 0 1299 Tm 41.566667 0 Td 83.333333 Tz (40 ) Tj 30 0 0 30 0 1091 Tm 41.566667 0 Td 80.555556 Tz (45 ) Tj 30 0 0 30 0 883 Tm 41.6 0 Td 80.555556 Tz (50 ) Tj 30 0 0 30 0 675 Tm 41.6 0 Td 77.777778 Tz (55 ) Tj 30 0 0 30 0 467 Tm 41.633333 0 Td 77.777778 Tz (60 ) Tj 30 0 0 30 0 259 Tm 41.633333 0 Td 75 Tz (65 ) Tj 51.428571 0 0 51.428571 0 2969 Tm 33.541667 0 Td 77.777778 Tz (22 ) Tj 51.428571 0 0 51.428571 0 2915.600522 Tm 25.297222 0 Td 52.391975 Tz (occurs ) Tj 2.216667 0 Td 47.962963 Tz (early ) Tj 1.769444 0 Td 50.771605 Tz (within ) Tj 2.158333 0 Td 55.092593 Tz (a ) Tj 0.622222 0 Td 47.395833 Tz (fracture ) Tj 2.586111 0 Td 42.12963 Tz (site ) Tj 1.302778 0 Td 51.851852 Tz (and, ) Tj 1.575 0 Td 61.574074 Tz (more ) Tj 1.808333 0 Td 43.625356 Tz (specifically, ) Tj 51.428571 0 0 51.428571 0 2874.467005 Tm 25.277778 0 Td 52.572016 Tz (hematoma-initiated ) Tj 6.416667 0 Td 53.472222 Tz (inflammation ) Tj 4.588889 0 Td 59.41358 Tz (and ) Tj 1.769444 0 Td 52.5 Tz (angiogenic-type ) Tj 40 0 0 40 0 2838 Tm 32.5 0 Td 64.166667 Tz (behaviors. ) Tj 52.857143 0 0 52.857143 0 2790.934726 Tm 25.332432 0 Td 47.297297 Tz (In ) Tj 0.775676 0 Td 54.864865 Tz (FIGS. ) Tj 1.835135 0 Td 63.851351 Tz (8D-E ) Tj 1.740541 0 Td 60.960961 Tz (F1b ) Tj 1.437838 0 Td 57.807808 Tz (and ) Tj 1.210811 0 Td 63.063063 Tz (F2b ) Tj 1.475676 0 Td 53.603604 Tz (groups ) Tj 2.081081 0 Td 47.034535 Tz (consistently ) Tj 3.556757 0 Td 71.891892 Tz (demon ) Tj 40 0 0 40 0 2755 Tm 32.55 0 Td 58.928571 Tz (strated ) Tj 2.675 0 Td 70.833333 Tz (a ) Tj 0.625 0 Td 70.075758 Tz (well-formed ) Tj 4.9 0 Td 72.02381 Tz (network ) Tj 3.2 0 Td 72.916667 Tz (of ) Tj 0.975 0 Td 69.270833 Tz (branches ) Tj 3.55 0 Td 75.833333 Tz (which ) Tj 2.525 0 Td 66.666667 Tz (terminated ) Tj 51.428571 0 0 51.428571 0 2707.196078 Tm 25.297222 0 Td 45.37037 Tz (at ) Tj 0.641667 0 Td 50.231481 Tz (junction ) Tj 2.586111 0 Td 46.296296 Tz (points; ) Tj 2.138889 0 Td 42.939815 Tz (this ) Tj 1.225 0 Td 50.673401 Tz (observation ) Tj 3.519444 0 Td 61.574074 Tz (was ) Tj 1.283333 0 Td 48.611111 Tz (similar ) Tj 2.138889 0 Td 48.611111 Tz (to ) Tj 0.758333 0 Td 45.37037 Tz (that ) Tj 1.263889 0 Td 50.771605 Tz (see ) Tj 1.088889 0 Td 47.530864 Tz (for ) Tj 51.428571 0 0 51.428571 0 2666.039422 Tm 25.297222 0 Td 72.916667 Tz (M200 ) Tj 2.041667 0 Td 58.333333 Tz (b ) Tj 0.738889 0 Td 65.625 Tz (BBE. ) Tj 1.847222 0 Td 55.497685 Tz (Branches ) Tj 2.936111 0 Td 48.611111 Tz (for ) Tj 1.127778 0 Td 48.611111 Tz (the ) Tj 1.127778 0 Td 52.391975 Tz (experimental ) Tj 4.083333 0 Td 62.654321 Tz (F1b ) Tj 1.458333 0 Td 60.493827 Tz (and ) Tj 1.361111 0 Td 59.953704 Tz (F2 ) Tj 51.428571 0 0 51.428571 0 2625.439901 Tm 25.375 0 Td 55.092593 Tz (b ) Tj 0.661111 0 Td 55.632716 Tz (groups ) Tj 2.236111 0 Td 46.8107 Tz (typically ) Tj 2.780556 0 Td 59.143519 Tz (were ) Tj 1.613889 0 Td 49.074074 Tz (thicker ) Tj 2.275 0 Td 45.37037 Tz (in ) Tj 0.777778 0 Td 50.987654 Tz (diameter/width, ) Tj 4.822222 0 Td 52.777778 Tz (qualita ) Tj 51.428571 0 0 51.428571 0 2583.069498 Tm 25.277778 0 Td 41.666667 Tz (tively. ) Tj 2.080556 0 Td 54.282407 Tz (F1-, ) Tj 1.594444 0 Td 53.472222 Tz (F2-, ) Tj 1.536111 0 Td 60.493827 Tz (and ) Tj 1.341667 0 Td 72.106481 Tz (M200 ) Tj 1.963889 0 Td 51.388889 Tz (groups, ) Tj 2.45 0 Td 58.333333 Tz (which ) Tj 2.002778 0 Td 51.851852 Tz (did ) Tj 1.205556 0 Td 51.851852 Tz (not ) Tj 1.147222 0 Td 51.851852 Tz (contain ) Tj 51.428571 0 0 51.428571 0 2544.990244 Tm 25.297222 0 Td 56.17284 Tz (growth ) Tj 2.255556 0 Td 44.965278 Tz (factors, ) Tj 2.372222 0 Td 56.712963 Tz (produced ) Tj 2.916667 0 Td 52.932099 Tz (poorly ) Tj 2.138889 0 Td 58.873457 Tz (formed ) Tj 2.352778 0 Td 54.6875 Tz (networks ) Tj 2.838889 0 Td 60.493827 Tz (and ) Tj 1.283333 0 Td 72.376543 Tz (gen ) Tj 51.428571 0 0 51.428571 0 2503.875458 Tm 25.297222 0 Td 45.37037 Tz (erally ) Tj 1.788889 0 Td 47.530864 Tz (retracted ) Tj 2.702778 0 Td 46.180556 Tz (into ) Tj 1.263889 0 Td 42.939815 Tz (cell ) Tj 1.205556 0 Td 52.777778 Tz (masses, ) Tj 2.352778 0 Td 53.472222 Tz (seen ) Tj 1.438889 0 Td 45.37037 Tz (in ) Tj 0.7 0 Td 45.37037 Tz (\(E\). ) Tj 1.244444 0 Td 72.916667 Tz (On ) Tj 1.05 0 Td 49.259259 Tz (close ) Tj 1.613889 0 Td 58.333333 Tz (inspec ) Tj 51.428571 0 0 51.428571 0 2464.673469 Tm 25.277778 0 Td 46.990741 Tz (tion ) Tj 1.4 0 Td 56.712963 Tz (of ) Tj 0.836111 0 Td 44.560185 Tz (\(E\), ) Tj 1.302778 0 Td 35.648148 Tz (it ) Tj 0.641667 0 Td 38.888889 Tz (is ) Tj 0.7 0 Td 52.662037 Tz (apparent ) Tj 2.722222 0 Td 46.990741 Tz (that ) Tj 1.380556 0 Td 47.530864 Tz (multiple, ) Tj 2.8 0 Td 51.851852 Tz (Small ) Tj 1.769444 0 Td 53.877315 Tz (branches ) Tj 2.838889 0 Td 46.180556 Tz (that ) Tj 51.428571 0 0 51.428571 0 2425.002454 Tm 25.297222 0 Td 49.259259 Tz (often ) Tj 1.691667 0 Td 51.851852 Tz (terminated ) Tj 3.286111 0 Td 59.953704 Tz (on ) Tj 0.933333 0 Td 49.691358 Tz (the ) Tj 1.069444 0 Td 48.611111 Tz (gel ) Tj 1.069444 0 Td 43.209877 Tz (itself ) Tj 1.633333 0 Td 59.953704 Tz (were ) Tj 1.633333 0 Td 52.662037 Tz (seen ) Tj 1.458333 0 Td 51.527778 Tz (protruding ) Tj 3.286111 0 Td 59.143519 Tz (from ) Tj 51.428571 0 0 51.428571 0 2385.972521 Tm 25.297222 0 Td 48.935185 Tz (individual ) Tj 3.013889 0 Td 50.05144 Tz (junctions ) Tj 2.858333 0 Td 57.523148 Tz (from ) Tj 1.516667 0 Td 48.611111 Tz (the ) Tj 1.030556 0 Td 42.939815 Tz (cell ) Tj 1.186111 0 Td 56.17284 Tz (masses ) Tj 2.138889 0 Td 59.41358 Tz (and ) Tj 1.205556 0 Td 50.462963 Tz (explain ) Tj 2.255556 0 Td 68.055556 Tz (why ) Tj 1.361111 0 Td 50.771605 Tz (the ) Tj 51.428571 0 0 51.428571 0 2345.923172 Tm 25.297222 0 Td 62.654321 Tz (number ) Tj 2.430556 0 Td 56.712963 Tz (of ) Tj 0.777778 0 Td 53.877315 Tz (branches ) Tj 2.761111 0 Td 60.493827 Tz (and ) Tj 1.225 0 Td 50.05144 Tz (junctions ) Tj 2.916667 0 Td 59.143519 Tz (were ) Tj 1.613889 0 Td 51.851852 Tz (not ) Tj 1.108333 0 Td 57.037037 Tz (found ) Tj 1.886111 0 Td 46.990741 Tz (to ) Tj 0.777778 0 Td 56.712963 Tz (be ) Tj 0.894444 0 Td 59.41358 Tz (sig ) Tj 51.428571 0 0 51.428571 0 2308.121212 Tm 25.297222 0 Td 46.018519 Tz (nificantly ) Tj 2.936111 0 Td 48.611111 Tz (affected ) Tj 2.488889 0 Td 59.953704 Tz (by ) Tj 0.933333 0 Td 49.691358 Tz (the ) Tj 1.108333 0 Td 49.074074 Tz (various ) Tj 2.255556 0 Td 50.925926 Tz (groups. ) Tj 51.428571 0 0 51.428571 0 2267.41791 Tm 26.036111 0 Td 54.166667 Tz (Healthy ) Tj 2.469444 0 Td 52.146465 Tz (reformation ) Tj 3.655556 0 Td 55.092593 Tz (of ) Tj 0.758333 0 Td 59.143519 Tz (bone ) Tj 1.613889 0 Td 58.333333 Tz (and ) Tj 1.263889 0 Td 48.179012 Tz (vascularization ) Tj 4.530556 0 Td 51.041667 Tz (adjacent ) Tj 51.428571 0 0 51.428571 0 2232.18408 Tm 25.277778 0 Td 46.990741 Tz (to ) Tj 0.777778 0 Td 45.910494 Tz (joints ) Tj 1.905556 0 Td 49.259259 Tz (exhibiting ) Tj 3.188889 0 Td 47.962963 Tz (early ) Tj 1.691667 0 Td 48.611111 Tz (stages ) Tj 2.022222 0 Td 58.333333 Tz (of ) Tj 0.855556 0 Td 51.851852 Tz (degeneration ) Tj 3.966667 0 Td 52.256944 Tz (provides ) Tj 2.761111 0 Td 55.092593 Tz (a ) Tj 51.428571 0 0 51.428571 0 2188.90629 Tm 25.297222 0 Td 61.574074 Tz (means ) Tj 2.197222 0 Td 56.712963 Tz (of ) Tj 0.894444 0 Td 49.691358 Tz (reversing ) Tj 3.013889 0 Td 41.049383 Tz (or, ) Tj 1.069444 0 Td 46.990741 Tz (inhibiting ) Tj 3.130556 0 Td 49.691358 Tz (the ) Tj 1.186111 0 Td 58.627946 Tz (advancement ) Tj 4.161111 0 Td 58.333333 Tz (of ) Tj 0.913889 0 Td 50.771605 Tz (the ) Tj 51.428571 0 0 51.428571 0 2150.169412 Tm 25.297222 0 Td 48.905724 Tz (age-related ) Tj 3.441667 0 Td 50 Tz (disease ) Tj 2.275 0 Td 44.560185 Tz (\(osteoarthritis\) ) Tj 4.472222 0 Td 53.703704 Tz (through ) Tj 2.469444 0 Td 52.175926 Tz (addressing ) Tj 3.286111 0 Td 51.203704 Tz (these ) Tj 51.428571 0 0 51.428571 0 2110.105882 Tm 25.297222 0 Td 50.084175 Tz (detrimental ) Tj 3.422222 0 Td 59.953704 Tz (bone ) Tj 1.555556 0 Td 52.256944 Tz (changes. ) Tj 2.683333 0 Td 50.106838 Tz (Incorporation ) Tj 4.044444 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 72.916667 Tz (hCPR ) Tj 1.886111 0 Td 50.771605 Tz (within ) Tj 2.002778 0 Td 62.654321 Tz (F1+ ) Tj 51.428571 0 0 51.428571 0 2071.368862 Tm 25.297222 0 Td 58.333333 Tz (and ) Tj 1.302778 0 Td 62.654321 Tz (F2+ ) Tj 1.380556 0 Td 49.293372 Tz (alginate-containing ) Tj 5.852778 0 Td 50.084175 Tz (biomaterial ) Tj 3.558333 0 Td 54.12037 Tz (composites ) Tj 3.480556 0 Td 52.391975 Tz (allows ) Tj 51.428571 0 0 51.428571 0 2031.001183 Tm 25.297222 0 Td 48.611111 Tz (for ) Tj 1.030556 0 Td 49.259259 Tz (controlled ) Tj 3.111111 0 Td 48.148148 Tz (release ) Tj 2.216667 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 53.148148 Tz (autologous ) Tj 3.344444 0 Td 49.583333 Tz (bioagents. ) Tj 3.130556 0 Td 64.814815 Tz (The ) Tj 1.322222 0 Td 47.530864 Tz (releasate ) Tj 51.428571 0 0 51.428571 0 1990.07 Tm 25.316667 0 Td 55.902778 Tz (Supplemented ) Tj 4.394444 0 Td 50.771605 Tz (the ) Tj 1.302778 0 Td 50.231481 Tz (inherent ) Tj 2.819444 0 Td 51.041667 Tz (material-induced ) Tj 5.288889 0 Td 43.75 Tz (initiation ) Tj 3.052778 0 Td 56.712963 Tz (of ) Tj 51.428571 0 0 51.428571 0 1950.416867 Tm 25.297222 0 Td 59.548611 Tz (monocyte ) Tj 3.033333 0 Td 59.41358 Tz (and ) Tj 1.263889 0 Td 48.905724 Tz (endothelial ) Tj 3.422222 0 Td 42.939815 Tz (cell ) Tj 1.263889 0 Td 50.673401 Tz (recruitment ) Tj 3.5 0 Td 46.990741 Tz (that ) Tj 1.322222 0 Td 38.888889 Tz (is ) Tj 0.661111 0 Td 50.231481 Tz (associated ) Tj 51.428571 0 0 51.428571 0 1911.486624 Tm 25.277778 0 Td 54.282407 Tz (with ) Tj 1.594444 0 Td 49.691358 Tz (the ) Tj 1.186111 0 Td 47.962963 Tz (early ) Tj 1.769444 0 Td 52.146465 Tz (occurrences ) Tj 3.733333 0 Td 58.333333 Tz (of ) Tj 0.875 0 Td 59.953704 Tz (bone ) Tj 1.730556 0 Td 45.910494 Tz (tissue ) Tj 1.944444 0 Td 55.092593 Tz (remodeling ) Tj 3.577778 0 Td 60.493827 Tz (and ) Tj 51.428571 0 0 51.428571 0 1872.24 Tm 25.297222 0 Td 54.62963 Tz (induced ) Tj 2.605556 0 Td 58.333333 Tz (a ) Tj 0.641667 0 Td 51.851852 Tz (well-organized ) Tj 4.647222 0 Td 49.200337 Tz (endothelial ) Tj 3.577778 0 Td 51.851852 Tz (tube ) Tj 1.594444 0 Td 51.49177 Tz (networks. ) Tj 3.111111 0 Td 53.472222 Tz (This ) Tj 51.428571 0 0 51.428571 0 1832.895641 Tm 25.277778 0 Td 51.49177 Tz (treatment ) Tj 3.072222 0 Td 45.37037 Tz (in ) Tj 0.855556 0 Td 58.333333 Tz (a ) Tj 0.641667 0 Td 47.685185 Tz (patient ) Tj 2.333333 0 Td 66.975309 Tz (may ) Tj 1.536111 0 Td 50.231481 Tz (lead ) Tj 1.477778 0 Td 48.611111 Tz (to ) Tj 0.894444 0 Td 50.462963 Tz (healthy ) Tj 2.45 0 Td 53.703704 Tz (osseous ) Tj 2.566667 0 Td 46.450617 Tz (tissue ) Tj 51.428571 0 0 51.428571 0 1793.643478 Tm 25.297222 0 Td 50.231481 Tz (regeneration ) Tj 3.830556 0 Td 58.333333 Tz (and ) Tj 1.263889 0 Td 55.092593 Tz (be ) Tj 0.875 0 Td 50.231481 Tz (associated ) Tj 3.227778 0 Td 52.662037 Tz (with ) Tj 1.477778 0 Td 50.771605 Tz (the ) Tj 1.108333 0 Td 51.851852 Tz (delay ) Tj 1.769444 0 Td 53.472222 Tz (or ) Tj 0.855556 0 Td 46.585648 Tz (reversal ) Tj 2.45 0 Td 58.333333 Tz (of ) Tj 51.428571 0 0 51.428571 0 1758.134872 Tm 25.258333 0 Td 46.018519 Tz (joint ) Tj 1.633333 0 Td 51.851852 Tz (degeneration ) Tj 3.966667 0 Td 54.166667 Tz (through ) Tj 2.547222 0 Td 49.96142 Tz (rejuvenation ) Tj 3.869444 0 Td 58.333333 Tz (of ) Tj 0.855556 0 Td 42.939815 Tz (this ) Tj 1.322222 0 Td 49.789562 Tz (underlying, ) Tj 51.428571 0 0 51.428571 0 1716.892562 Tm 25.277778 0 Td 53.292181 Tz (unhealthy ) Tj 3.052778 0 Td 43.518519 Tz (tissue. ) Tj 52.857143 0 0 52.857143 0 1641.052632 Tm 31.575676 0 Td 60.36036 Tz (Example ) Tj 2.686486 0 Td 56.756757 Tz (3 ) Tj 51.428571 0 0 51.428571 0 1552.673267 Tm 26.016667 0 Td 57.685185 Tz (There ) Tj 1.808333 0 Td 46.450617 Tz (exists ) Tj 1.788889 0 Td 58.333333 Tz (a ) Tj 0.486111 0 Td 44.155093 Tz (clinical ) Tj 2.313889 0 Td 55.902778 Tz (need ) Tj 1.458333 0 Td 48.611111 Tz (for ) Tj 0.991667 0 Td 48.611111 Tz (consistent ) Tj 3.033333 0 Td 59.143519 Tz (bone ) Tj 1.575 0 Td 54.768519 Tz (remodeling ) Tj 51.428571 0 0 51.428571 0 1512.748474 Tm 25.277778 0 Td 51.851852 Tz (within ) Tj 2.061111 0 Td 52.73569 Tz (problematic ) Tj 3.675 0 Td 42.777778 Tz (sites ) Tj 1.497222 0 Td 55.092593 Tz (such ) Tj 1.536111 0 Td 48.611111 Tz (as ) Tj 0.797222 0 Td 51.203704 Tz (those ) Tj 1.75 0 Td 58.333333 Tz (of ) Tj 0.797222 0 Td 46.990741 Tz (fracture ) Tj 2.45 0 Td 53.652263 Tz (nonunion, ) Tj 51.428571 0 0 51.428571 0 1473.256039 Tm 25.297222 0 Td 50.771605 Tz (avascular ) Tj 2.955556 0 Td 46.8107 Tz (necrosis, ) Tj 2.722222 0 Td 53.472222 Tz (or ) Tj 0.816667 0 Td 46.8107 Tz (irregular ) Tj 2.683333 0 Td 59.953704 Tz (bone ) Tj 1.652778 0 Td 49.789562 Tz (formations. ) Tj 3.480556 0 Td 63.194444 Tz (To ) Tj 0.972222 0 Td 51.851852 Tz (address ) Tj 51.428571 0 0 51.428571 0 1436.47619 Tm 25.316667 0 Td 54.282407 Tz (Such ) Tj 1.477778 0 Td 49.691358 Tz (needs, ) Tj 1.963889 0 Td 55.092593 Tz (a ) Tj 0.447222 0 Td 50.084175 Tz (biomaterial ) Tj 3.5 0 Td 55.092593 Tz (composite ) Tj 3.130556 0 Td 53.914141 Tz (composition ) Tj 3.733333 0 Td 38.888889 Tz (is ) Tj 0.602778 0 Td 54.282407 Tz (provided ) Tj 51.428571 0 0 51.428571 0 1399.032138 Tm 25.277778 0 Td 46.990741 Tz (to ) Tj 0.894444 0 Td 53.472222 Tz (induce ) Tj 2.197222 0 Td 48.611111 Tz (early ) Tj 1.769444 0 Td 54.012346 Tz (inflammatory ) Tj 4.2 0 Td 52.211934 Tz (responses ) Tj 3.111111 0 Td 46.018519 Tz (after ) Tj 1.633333 0 Td 51.311728 Tz (implantation ) Tj 51.428571 0 0 51.428571 0 1355.858586 Tm 25.297222 0 Td 58.333333 Tz (and ) Tj 1.361111 0 Td 45.37037 Tz (to ) Tj 0.836111 0 Td 53.240741 Tz (provide ) Tj 2.566667 0 Td 43.425926 Tz (later ) Tj 1.555556 0 Td 52.5 Tz (osteoconductive ) Tj 5.016667 0 Td 49.494949 Tz (scaffolding ) Tj 3.577778 0 Td 46.450617 Tz (for ) Tj 1.127778 0 Td 59.953704 Tz (bone ) Tj 51.428571 0 0 51.428571 0 1318.481871 Tm 25.297222 0 Td 48.361823 Tz (regeneration. ) Tj 4.102778 0 Td 51.557239 Tz (Biomaterial ) Tj 3.675 0 Td 54.330065 Tz (composite-induced ) Tj 5.813889 0 Td 54.282407 Tz (inflammation ) Tj 51.428571 0 0 51.428571 0 1277.440658 Tm 25.297222 0 Td 45.37037 Tz (parallels ) Tj 2.586111 0 Td 50.771605 Tz (the ) Tj 1.030556 0 Td 49.259259 Tz (early ) Tj 1.652778 0 Td 49.259259 Tz (stage ) Tj 1.633333 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 58.130787 Tz (hematoma-induced ) Tj 5.716667 0 Td 47.395833 Tz (fracture ) Tj 2.430556 0 Td 47.530864 Tz (repair ) Tj 51.428571 0 0 51.428571 0 1240.576037 Tm 25.297222 0 Td 58.333333 Tz (and ) Tj 1.166667 0 Td 51.311728 Tz (allows ) Tj 2.002778 0 Td 52.423747 Tz (scaffold-promoted ) Tj 5.425 0 Td 56.28655 Tz (remodelingofosseous ) Tj 6.513889 0 Td 46.990741 Tz (tissue ) Tj 1.808333 0 Td 43.75 Tz (to ) Tj 51.428571 0 0 51.428571 0 1196.443099 Tm 25.297222 0 Td 55.092593 Tz (a ) Tj 0.447222 0 Td 50.231481 Tz (health ) Tj 1.963889 0 Td 41.049383 Tz (State. ) Tj 1.672222 0 Td 44.074074 Tz (Initiation ) Tj 2.819444 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 50.771605 Tz (the ) Tj 1.05 0 Td 65.462963 Tz (wound ) Tj 2.1 0 Td 51.388889 Tz (healing ) Tj 2.294444 0 Td 54.62963 Tz (cascade ) Tj 2.391667 0 Td 61.574074 Tz (by ) Tj 0.894444 0 Td 59.41358 Tz (two ) Tj 51.428571 0 0 51.428571 0 1161.314879 Tm 25.277778 0 Td 65.462963 Tz (human ) Tj 2.177778 0 Td 51.311728 Tz (concentrated ) Tj 3.830556 0 Td 45.37037 Tz (platelet ) Tj 2.313889 0 Td 48.935185 Tz (releasate-containing ) Tj 6.008333 0 Td 47.727273 Tz (alginate/B- ) Tj 51.428571 0 0 51.428571 0 1119.533333 Tm 25.277778 0 Td 49.259259 Tz (tricalcium ) Tj 3.091667 0 Td 55.452675 Tz (phosphate ) Tj 3.111111 0 Td 54.095442 Tz (biocomposites ) Tj 4.375 0 Td 52.932099 Tz (has ) Tj 1.108333 0 Td 56.712963 Tz (been ) Tj 1.555556 0 Td 47.222222 Tz (studies ) Tj 2.158333 0 Td 45.37037 Tz (in ) Tj 0.738889 0 Td 44.074074 Tz (vitro ) Tj 51.428571 0 0 51.428571 0 1079.292804 Tm 25.277778 0 Td 53.796296 Tz (using ) Tj 1.788889 0 Td 48.611111 Tz (the ) Tj 1.030556 0 Td 59.953704 Tz (TIB-71TM ) Tj 3.072222 0 Td 67.650463 Tz (RAW264.7 ) Tj 3.422222 0 Td 64.166667 Tz (mouse ) Tj 2.080556 0 Td 59.953704 Tz (monocyte ) Tj 3.033333 0 Td 42.939815 Tz (cell ) Tj 1.263889 0 Td 40.833333 Tz (line. ) Tj 51.428571 0 0 51.428571 0 1038.455587 Tm 26.016667 0 Td 53.472222 Tz (This ) Tj 1.730556 0 Td 46.990741 Tz (in ) Tj 1.030556 0 Td 44.722222 Tz (vitro ) Tj 1.769444 0 Td 58.796296 Tz (example ) Tj 2.936111 0 Td 44.444444 Tz (relates ) Tj 2.313889 0 Td 45.37037 Tz (to ) Tj 1.011111 0 Td 50.925926 Tz (tunable ) Tj 2.586111 0 Td 46.720679 Tz (interactions ) Tj 51.428571 0 0 51.428571 0 1000.328502 Tm 25.277778 0 Td 57.87037 Tz (between ) Tj 2.644444 0 Td 49.691358 Tz (the ) Tj 1.069444 0 Td 45.37037 Tz (platelet ) Tj 2.391667 0 Td 46.666667 Tz (releasates ) Tj 2.994444 0 Td 59.41358 Tz (and ) Tj 1.263889 0 Td 49.691358 Tz (the ) Tj 1.088889 0 Td 50.378788 Tz (biomaterial ) Tj 3.519444 0 Td 69.675926 Tz (compos ) Tj 51.428571 0 0 51.428571 0 960.390361 Tm 25.297222 0 Td 41.319444 Tz (ites ) Tj 1.244444 0 Td 48.611111 Tz (for ) Tj 1.05 0 Td 55.812757 Tz (enhancing ) Tj 3.247222 0 Td 55.840456 Tz (hematoma-like ) Tj 4.569444 0 Td 47.395833 Tz (fracture ) Tj 2.508333 0 Td 43.055556 Tz (repair. ) Tj 2.022222 0 Td 58.738426 Tz (Addition ) Tj 51.428571 0 0 51.428571 0 924.208633 Tm 25.297222 0 Td 40.833333 Tz (ally, ) Tj 1.380556 0 Td 54.372428 Tz (minimally ) Tj 3.111111 0 Td 48.611111 Tz (invasive ) Tj 2.469444 0 Td 48.206019 Tz (delivery ) Tj 2.45 0 Td 48.611111 Tz (for ) Tj 0.972222 0 Td 45.37037 Tz (in ) Tj 0.719444 0 Td 42.12963 Tz (situ ) Tj 1.147222 0 Td 51.851852 Tz (curing ) Tj 1.983333 0 Td 49.691358 Tz (the ) Tj 1.030556 0 Td 52.777778 Tz (implant ) Tj 51.428571 0 0 51.428571 0 881.343434 Tm 25.316667 0 Td 54.552469 Tz (system ) Tj 2.275 0 Td 48.611111 Tz (via ) Tj 1.108333 0 Td 47.170782 Tz (injection ) Tj 2.8 0 Td 62.654321 Tz (was ) Tj 1.380556 0 Td 54.552469 Tz (demonstrated ) Tj 4.161111 0 Td 46.990741 Tz (in ) Tj 0.816667 0 Td 44.290123 Tz (rat ) Tj 1.011111 0 Td 38.888889 Tz (tail ) Tj 1.244444 0 Td 49.331276 Tz (vertebrae ) Tj 51.428571 0 0 51.428571 0 846.632207 Tm 25.277778 0 Td 53.148148 Tz (using ) Tj 1.788889 0 Td 55.740741 Tz (micro ) Tj 1.866667 0 Td 59.953704 Tz (computed ) Tj 3.033333 0 Td 55.092593 Tz (tomography. ) Tj 51.428571 0 0 51.428571 0 807.357231 Tm 26.016667 0 Td 70.216049 Tz (Two ) Tj 1.536111 0 Td 46.990741 Tz (injectable ) Tj 3.052778 0 Td 48.611111 Tz (alginate ) Tj 2.586111 0 Td 50.308642 Tz (hydrogel/B-tricalcium ) Tj 6.611111 0 Td 55.092593 Tz (phosphate ) Tj 51.428571 0 0 51.428571 0 765.032147 Tm 25.297222 0 Td 52.314815 Tz (granule ) Tj 2.294444 0 Td 54.444444 Tz (composites ) Tj 3.402778 0 Td 51.203704 Tz (containing ) Tj 3.169444 0 Td 49.331276 Tz (bioactive ) Tj 2.8 0 Td 51.58179 Tz (concentrated ) Tj 3.791667 0 Td 67.407407 Tz (human ) Tj 51.428571 0 0 51.428571 0 723.765685 Tm 25.297222 0 Td 44.965278 Tz (platelet ) Tj 2.430556 0 Td 46.8107 Tz (releasate ) Tj 2.819444 0 Td 56.944444 Tz (\(hCPR\), ) Tj 2.683333 0 Td 51.527778 Tz (designated ) Tj 3.363889 0 Td 50.231481 Tz (as ) Tj 0.933333 0 Td 61.574074 Tz (F1+ ) Tj 1.4 0 Td 58.333333 Tz (and ) Tj 1.341667 0 Td 55.092593 Tz (F2+, ) Tj 1.575 0 Td 51.851852 Tz (are ) Tj 51.428571 0 0 51.428571 0 683.4489 Tm 25.297222 0 Td 51.851852 Tz (described ) Tj 3.091667 0 Td 43.75 Tz (in ) Tj 0.797222 0 Td 54.444444 Tz (Table ) Tj 1.983333 0 Td 32.407407 Tz (1 ) Tj 0.583333 0 Td 59.143519 Tz (were ) Tj 1.691667 0 Td 50.411523 Tz (prepared. ) Tj 3.013889 0 Td 61.574074 Tz (The ) Tj 1.419444 0 Td 51.311728 Tz (formulations ) Tj 3.966667 0 Td 51.851852 Tz (are ) Tj 51.428571 0 0 51.428571 0 647.267081 Tm 25.297222 0 Td 53.877315 Tz (designed ) Tj 2.819444 0 Td 55.092593 Tz (such ) Tj 1.555556 0 Td 46.990741 Tz (that ) Tj 1.322222 0 Td 51.851852 Tz (the ) Tj 1.147222 0 Td 48.206019 Tz (alginate ) Tj 2.547222 0 Td 53.703704 Tz (weights ) Tj 2.508333 0 Td 52.5 Tz (percentage ) Tj 3.383333 0 Td 58.333333 Tz (and ) Tj 1.283333 0 Td 50.771605 Tz (the ) Tj 51.428571 0 0 51.428571 0 607.707733 Tm 25.433333 0 Td 47.800926 Tz (B-tricalcium ) Tj 3.947222 0 Td 54.73251 Tz (phosphate ) Tj 3.461111 0 Td 60.493827 Tz (Volume ) Tj 2.644444 0 Td 52.824074 Tz (percentage ) Tj 3.655556 0 Td 40.509259 Tz (is ) Tj 1.011111 0 Td 48.251029 Tz (inversely ) Tj 51.428571 0 0 51.428571 0 566.333333 Tm 25.297222 0 Td 44.560185 Tz (related. ) Tj 2.333333 0 Td 72.916667 Tz (An ) Tj 1.108333 0 Td 46.843434 Tz (additional, ) Tj 3.344444 0 Td 51.041667 Tz (non-biologic ) Tj 3.908333 0 Td 51.851852 Tz (formulation ) Tj 3.655556 0 Td 52.175926 Tz (designated ) Tj 51.428571 0 0 51.428571 0 525.408313 Tm 25.316667 0 Td 63.734568 Tz (F2- ) Tj 1.302778 0 Td 61.574074 Tz (was ) Tj 1.263889 0 Td 49.537037 Tz (created ) Tj 2.236111 0 Td 46.450617 Tz (identical ) Tj 2.683333 0 Td 43.75 Tz (to ) Tj 0.7 0 Td 63.734568 Tz (F2+ ) Tj 1.322222 0 Td 51.851852 Tz (with ) Tj 1.438889 0 Td 48.611111 Tz (the ) Tj 1.011111 0 Td 51.851852 Tz (exception ) Tj 2.955556 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 42.12963 Tz (sterile ) Tj 51.428571 0 0 51.428571 0 485.356265 Tm 25.297222 0 Td 52.572016 Tz (deionized ) Tj 3.072222 0 Td 53.148148 Tz (water ) Tj 1.847222 0 Td 49.691358 Tz (replacing ) Tj 2.897222 0 Td 62.87037 Tz (hCPR. ) Tj 2.1 0 Td 64.814815 Tz (The ) Tj 1.4 0 Td 63.734568 Tz (F2- ) Tj 1.380556 0 Td 51.557239 Tz (formulation ) Tj 3.655556 0 Td 61.574074 Tz (was ) Tj 40 0 0 40 0 453 Tm 32.55 0 Td 62.5 Tz (selected ) Tj 3.325 0 Td 62.5 Tz (for ) Tj 1.45 0 Td 62.962963 Tz (inclusion ) Tj 3.75 0 Td 66.666667 Tz (as ) Tj 1.125 0 Td 75 Tz (a ) Tj 0.85 0 Td 62.5 Tz (material ) Tj 3.325 0 Td 63.690476 Tz (control ) Tj 3.05 0 Td 61.111111 Tz (for ) Tj 1.45 0 Td 62.5 Tz (the ) Tj 1.45 0 Td 63.690476 Tz (current ) Tj 51.428571 0 0 51.428571 0 408.620513 Tm 25.297222 0 Td 58.796296 Tz (example ) Tj 2.683333 0 Td 48.611111 Tz (as ) Tj 0.836111 0 Td 57.407407 Tz (opposed ) Tj 2.625 0 Td 45.37037 Tz (to ) Tj 0.797222 0 Td 55.092593 Tz (an ) Tj 0.913889 0 Td 64.814815 Tz (F1- ) Tj 1.4 0 Td 51.557239 Tz (formulation ) Tj 3.655556 0 Td 57.253086 Tz (due ) Tj 1.225 0 Td 48.611111 Tz (to ) Tj 0.797222 0 Td 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1.905556 0 Td 56.712963 Tz (Plasma ) Tj 2.216667 0 Td 59.41358 Tz (and ) Tj 1.205556 0 Td 51.203704 Tz (buffy ) Tj 1.730556 0 Td 51.041667 Tz (coat ) Tj 51.428571 0 0 51.428571 0 2424.468031 Tm 6.377778 0 Td 50.231481 Tz (Supernatants ) Tj 3.966667 0 Td 58.333333 Tz (were ) Tj 1.711111 0 Td 48.611111 Tz (collected ) Tj 2.936111 0 Td 46.018519 Tz (after ) Tj 1.672222 0 Td 55.092593 Tz (a ) Tj 0.622222 0 Td 49.691358 Tz (ten ) Tj 1.263889 0 Td 55.092593 Tz (minute ) Tj 2.333333 0 Td 55.092593 Tz (low-speed ) Tj 51.428571 0 0 51.428571 0 2383.483104 Tm 6.358333 0 Td 48.37963 Tz (centrifugation ) Tj 4.355556 0 Td 55.902778 Tz (\(600 ) Tj 1.594444 0 Td 42.12963 Tz (g\). ) Tj 1.088889 0 Td 44.650206 Tz (Platelets ) Tj 2.702778 0 Td 58.333333 Tz (were ) Tj 1.672222 0 Td 51.041667 Tz (then ) Tj 1.497222 0 Td 51.851852 Tz (concentrated ) Tj 3.986111 0 Td 50.231481 Tz (in ) Tj 51.428571 0 0 51.428571 0 2347.040189 Tm 6.358333 0 Td 56.712963 Tz (plasma ) Tj 2.177778 0 Td 59.953704 Tz (by ) Tj 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2.022222 0 Td 54.166667 Tz (calcium ) Tj 2.547222 0 Td 49.421296 Tz (chloride ) Tj 2.644444 0 Td 54.012346 Tz (\(50 ) Tj 1.263889 0 Td 49.907407 Tz (ul/ml ) Tj 1.75 0 Td 56.712963 Tz (of ) Tj 0.894444 0 Td 56.388889 Tz (PRP\). ) Tj 1.925 0 Td 53.472222 Tz (Clot ) Tj 1.555556 0 Td 53.292181 Tz (formation ) Tj 51.428571 0 0 51.428571 0 2106.257596 Tm 6.338889 0 Td 62.654321 Tz (was ) Tj 1.380556 0 Td 55.092593 Tz (allowed ) Tj 2.566667 0 Td 46.990741 Tz (to ) Tj 0.836111 0 Td 55.092593 Tz (proceed ) Tj 2.586111 0 Td 47.530864 Tz (for ) Tj 1.088889 0 Td 58.333333 Tz (one ) Tj 1.283333 0 Td 58.333333 Tz (hour ) Tj 1.594444 0 Td 46.990741 Tz (at ) Tj 0.797222 0 Td 56.17284 Tz (37 ) Tj 1.283333 0 Td 55.092593 Tz (C. ) Tj 0.933333 0 Td 72.916667 Tz (hCPR ) Tj 2.022222 0 Td 61.574074 Tz (was ) Tj 51.428571 0 0 51.428571 0 2067.313725 Tm 6.358333 0 Td 51.041667 Tz (expelled ) Tj 2.625 0 Td 58.333333 Tz (from ) Tj 1.594444 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 42.12963 Tz (clot, ) Tj 1.458333 0 Td 42.12963 Tz (sterile ) Tj 1.944444 0 Td 50.925926 Tz (syringe ) Tj 2.313889 0 Td 42.939815 Tz (filtered ) Tj 2.236111 0 Td 50.555556 Tz (\(0.20 ) Tj 1.730556 0 Td 52.662037 Tz (um\), ) Tj 1.438889 0 Td 61.574074 Tz (and ) Tj 40 0 0 40 0 2034 Tm 8.2 0 Td 63.194444 Tz (stored ) Tj 2.475 0 Td 58.333333 Tz (at ) Tj 0.925 0 Td 75 Tz (-80 ) Tj 2.075 0 Td 68.75 Tz (C. ) Tj 1.1 0 Td 55.833333 Tz (until ) Tj 1.925 0 Td 69.791667 Tz (time ) Tj 1.925 0 Td 72.916667 Tz (of ) Tj 0.975 0 Td 60.416667 Tz (use. ) Tj 51.428571 0 0 51.428571 0 1989.003995 Tm 7.097222 0 Td 62.654321 Tz (The ) Tj 1.361111 0 Td 46.990741 Tz (in ) Tj 0.836111 0 Td 44.722222 Tz (vitro ) Tj 1.613889 0 Td 42.12963 Tz (test ) Tj 1.244444 0 Td 55.812757 Tz (specimens ) Tj 3.266667 0 Td 59.953704 Tz (of ) Tj 0.875 0 Td 47.530864 Tz (the ) Tj 1.108333 0 Td 49.537037 Tz (current ) Tj 2.294444 0 Td 58.796296 Tz (example ) Tj 2.722222 0 Td 59.143519 Tz (were ) Tj 51.428571 0 0 51.428571 0 1951.032345 Tm 6.358333 0 Td 66.435185 Tz (made ) Tj 1.905556 0 Td 59.953704 Tz (by ) Tj 1.05 0 Td 51.388889 Tz (placing ) Tj 2.547222 0 Td 46.450617 Tz (1.5 ) Tj 1.205556 0 Td 63.194444 Tz (ml ) Tj 1.088889 0 Td 59.953704 Tz (of ) Tj 0.972222 0 Td 58.333333 Tz (sodium ) Tj 2.430556 0 Td 48.206019 Tz (alginate ) Tj 2.663889 0 Td 57.79321 Tz (\(Sigma ) Tj 2.469444 0 Td 63.734568 Tz (PIN ) Tj 51.428571 0 0 51.428571 0 1908.097204 Tm 6.358333 0 Td 55.632716 Tz (71238; ) Tj 2.333333 0 Td 43.209877 Tz (St. ) Tj 1.108333 0 Td 50.771605 Tz (Louis, ) Tj 2.158333 0 Td 59.143519 Tz (Mo.\) ) Tj 1.730556 0 Td 48.206019 Tz (solution ) Tj 2.625 0 Td 45.37037 Tz (at ) Tj 0.875 0 Td 51.851852 Tz (twice ) Tj 1.886111 0 Td 48.611111 Tz (the ) Tj 1.166667 0 Td 42.777778 Tz (final ) Tj 1.594444 0 Td 55.632716 Tz (weight ) Tj 51.428571 0 0 51.428571 0 1874.787879 Tm 6.338889 0 Td 53.148148 Tz (percentage ) Tj 3.402778 0 Td 45.37037 Tz (in ) Tj 0.777778 0 Td 51.851852 Tz (a ) Tj 0.525 0 Td 58.333333 Tz (3 ) Tj 0.622222 0 Td 50.231481 Tz (cc ) Tj 0.875 0 Td 47.800926 Tz (Syringe. ) Tj 2.488889 0 Td 87.5 Tz (A ) Tj 0.758333 0 Td 49.826389 Tz (separate ) Tj 2.625 0 Td 50.462963 Tz (Syringe ) Tj 2.352778 0 Td 51.203704 Tz (containing ) Tj 51.428571 0 0 51.428571 0 1830.647619 Tm 6.436111 0 Td 56.018519 Tz (125-150 ) Tj 2.527778 0 Td 71.296296 Tz (um ) Tj 1.147222 0 Td 47.800926 Tz (B-tricalcium ) Tj 3.558333 0 Td 54.73251 Tz (phosphate ) Tj 3.111111 0 Td 51.041667 Tz (granules ) Tj 2.586111 0 Td 56.018519 Tz (\(Plasma ) Tj 2.508333 0 Td 45.833333 Tz (Biotal; ) Tj 51.428571 0 0 51.428571 0 1793.659232 Tm 6.377778 0 Td 51.262626 Tz (Derbyshire, ) Tj 3.636111 0 Td 59.953704 Tz (UK\), ) Tj 1.711111 0 Td 49.691358 Tz (the ) Tj 1.147222 0 Td 57.037037 Tz (major ) Tj 1.944444 0 Td 45.37037 Tz (setting ) Tj 2.177778 0 Td 50.925926 Tz (reagent ) Tj 2.352778 0 Td 54.62963 Tz (calcium ) Tj 2.566667 0 Td 45.833333 Tz (sulfate ) Tj 51.428571 0 0 51.428571 0 1751.653514 Tm 6.358333 0 Td 50.771605 Tz (dihydrate ) Tj 2.994444 0 Td 46.450617 Tz (\(as ) Tj 1.108333 0 Td 78.858025 Tz (USG ) Tj 1.652778 0 Td 80.37037 Tz (TERRA ) Tj 2.605556 0 Td 81.018519 Tz (ALBA ) Tj 2.197222 0 Td 52.777778 Tz (\(United ) Tj 2.469444 0 Td 47.530864 Tz (States ) Tj 1.963889 0 Td 69.135802 Tz (Gypsum ) Tj 51.428571 0 0 51.428571 0 1712.824691 Tm 6.358333 0 Td 62.384259 Tz (Company; ) Tj 3.188889 0 Td 53.472222 Tz (Chicago, ) Tj 2.780556 0 Td 34.722222 Tz (Ill.\)\), ) Tj 1.613889 0 Td 59.41358 Tz (and ) Tj 1.302778 0 Td 45.37037 Tz (1.5 ) Tj 1.011111 0 Td 61.574074 Tz (ml ) Tj 0.913889 0 Td 55.092593 Tz (of ) Tj 0.758333 0 Td 72.916667 Tz (hCPR ) Tj 1.925 0 Td 49.907407 Tz (\(with ) Tj 1.652778 0 Td 55.092593 Tz (or ) Tj 0.777778 0 Td 64.00463 Tz (with ) Tj 51.428571 0 0 51.428571 0 1670.79521 Tm 6.358333 0 Td 52.932099 Tz (out ) Tj 1.069444 0 Td 74.537037 Tz (USP ) Tj 1.477778 0 Td 53.796296 Tz (grade ) Tj 1.769444 0 Td 58.873457 Tz (sodium ) Tj 2.275 0 Td 43.518519 Tz (citrate ) Tj 1.944444 0 Td 51.851852 Tz (\(Mallinckrodt ) Tj 4.238889 0 Td 57.037037 Tz (Baker ) Tj 1.866667 0 Td 41.481481 Tz (Inc.; ) Tj 1.438889 0 Td 57.523148 Tz (Phil ) Tj 52.857143 0 0 52.857143 0 1635.221675 Tm 6.186486 0 Td 45.545546 Tz (lipsburg, ) Tj 2.686486 0 Td 45.195195 Tz (N.J.\)\) ) Tj 1.835135 0 Td 59.90991 Tz (was ) Tj 1.286486 0 Td 49.662162 Tz (then ) Tj 1.418919 0 Td 54.054054 Tz (coupled ) Tj 2.459459 0 Td 45.720721 Tz (to ) Tj 0.775676 0 Td 49.399399 Tz (the ) Tj 1.078378 0 Td 47.297297 Tz (alginate ) Tj 2.440541 0 Td 50.45045 Tz (containing ) Tj 51.428571 0 0 51.428571 0 1596.086124 Tm 6.377778 0 Td 47.800926 Tz (Syringe, ) Tj 2.469444 0 Td 53.472222 Tz (followed ) Tj 2.683333 0 Td 61.574074 Tz (by ) Tj 0.933333 0 Td 56.17284 Tz (mixing ) Tj 2.197222 0 Td 55.092593 Tz (\(~15 ) Tj 1.497222 0 Td 46.990741 Tz (passes\). ) Tj 2.430556 0 Td 66.435185 Tz (Upon ) Tj 1.769444 0 Td 64.09465 Tz (homogeniz ) Tj 51.428571 0 0 51.428571 0 1554.331695 Tm 6.358333 0 Td 46.180556 Tz (ing, ) Tj 1.263889 0 Td 50.771605 Tz (the ) Tj 1.127778 0 Td 48.611111 Tz (material ) Tj 2.527778 0 Td 61.574074 Tz (was ) Tj 1.283333 0 Td 50.87963 Tz (positioned ) Tj 3.227778 0 Td 46.180556 Tz (into ) Tj 1.302778 0 Td 59.41358 Tz (one ) Tj 1.225 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 50.636574 Tz (Syringes ) Tj 2.605556 0 Td 60.493827 Tz (and ) Tj 51.428571 0 0 51.428571 0 1512.03354 Tm 6.358333 0 Td 54.62963 Tz (allowed ) Tj 2.469444 0 Td 45.37037 Tz (to ) Tj 0.777778 0 Td 51.041667 Tz (cure ) Tj 1.419444 0 Td 48.611111 Tz (for ) Tj 1.011111 0 Td 58.333333 Tz (a ) Tj 0.544444 0 Td 66.203704 Tz (minimum ) Tj 3.013889 0 Td 54.444444 Tz (often ) Tj 1.886111 0 Td 49.826389 Tz (minutes. ) Tj 2.605556 0 Td 51.203704 Tz (After ) Tj 1.691667 0 Td 48.148148 Tz (curing, ) Tj 51.428571 0 0 51.428571 0 1476.31068 Tm 6.338889 0 Td 50.771605 Tz (the ) Tj 1.088889 0 Td 47.800926 Tz (ejection ) Tj 2.45 0 Td 50.231481 Tz (port ) Tj 1.380556 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 50.771605 Tz (the ) Tj 1.088889 0 Td 50.925926 Tz (Syringe ) Tj 2.313889 0 Td 61.574074 Tz (was ) Tj 1.302778 0 Td 56.30787 Tz (removed, ) Tj 2.877778 0 Td 59.41358 Tz (and ) Tj 1.244444 0 Td 55.452675 Tz (composite ) Tj 51.428571 0 0 51.428571 0 1440 Tm 6.358333 0 Td 48.251029 Tz (expelled. ) Tj 51.428571 0 0 51.428571 0 1396.774359 Tm 7.097222 0 Td 49.691358 Tz (All ) Tj 1.108333 0 Td 45.37037 Tz (in ) Tj 0.777778 0 Td 44.074074 Tz (vitro ) Tj 1.536111 0 Td 55.812757 Tz (specimens ) Tj 3.208333 0 Td 58.333333 Tz (were ) Tj 1.555556 0 Td 53.472222 Tz (prepared ) Tj 2.741667 0 Td 52.5 Tz (using ) Tj 1.769444 0 Td 42.12963 Tz (sterile ) Tj 1.944444 0 Td 57.79321 Tz (materi ) Tj 51.428571 0 0 51.428571 0 1355.284302 Tm 6.358333 0 Td 40.509259 Tz (als. ) Tj 1.205556 0 Td 52.256944 Tz (Alginate ) Tj 2.761111 0 Td 48.251029 Tz (Solutions ) Tj 2.877778 0 Td 58.333333 Tz (were ) Tj 1.633333 0 Td 52.175926 Tz (autoclaved ) Tj 3.383333 0 Td 47.530864 Tz (for ) Tj 1.069444 0 Td 55.092593 Tz (twenty ) Tj 2.255556 0 Td 54.166667 Tz (minutes ) Tj 51.428571 0 0 51.428571 0 1317.304117 Tm 6.338889 0 Td 57.685185 Tz (under ) Tj 1.963889 0 Td 54.282407 Tz (slow ) Tj 1.633333 0 Td 51.388889 Tz (venting ) Tj 2.430556 0 Td 45.37037 Tz (in ) Tj 0.836111 0 Td 61.574074 Tz (40 ) Tj 1.05 0 Td 59.953704 Tz (ml ) Tj 0.972222 0 Td 48.206019 Tz (aliquots ) Tj 2.605556 0 Td 52.5 Tz (using ) Tj 1.847222 0 Td 61.574074 Tz (50 ) Tj 1.030556 0 Td 59.953704 Tz (ml ) Tj 1.011111 0 Td 50 Tz (conical ) Tj 51.428571 0 0 51.428571 0 1277.762887 Tm 6.358333 0 Td 53.846154 Tz (polypropylene ) Tj 4.569444 0 Td 47.530864 Tz (tubes. ) Tj 2.119444 0 Td 58.796296 Tz (Calcium ) Tj 2.877778 0 Td 42.777778 Tz (salts ) Tj 1.691667 0 Td 59.143519 Tz (were ) Tj 1.788889 0 Td 53.652263 Tz (preloaded ) Tj 3.305556 0 Td 46.180556 Tz (into ) Tj 51.428571 0 0 51.428571 0 1238.328671 Tm 6.377778 0 Td 49.826389 Tz (Syringes ) Tj 2.508333 0 Td 59.41358 Tz (and ) Tj 1.147222 0 Td 57.407407 Tz (exposed ) Tj 2.508333 0 Td 48.611111 Tz (to ) Tj 0.7 0 Td 55.092593 Tz (low-dose ) Tj 2.741667 0 Td 71.296296 Tz (gamma ) Tj 2.275 0 Td 44.020062 Tz (irradiation. ) Tj 3.305556 0 Td 63.194444 Tz (Sodium ) Tj 51.428571 0 0 51.428571 0 1195 Tm 6.358333 0 Td 43.981481 Tz (citrate ) Tj 2.080556 0 Td 46.180556 Tz (\(for ) Tj 1.341667 0 Td 63.734568 Tz (F2+ ) Tj 1.4 0 Td 58.333333 Tz (and ) Tj 1.322222 0 Td 58.333333 Tz (F2-\) ) Tj 1.652778 0 Td 61.574074 Tz (was ) Tj 1.361111 0 Td 42.592593 Tz (sterile ) Tj 2.022222 0 Td 42.939815 Tz (filtered ) Tj 2.313889 0 Td 50.231481 Tz (as ) Tj 0.836111 0 Td 51.851852 Tz (a ) Tj 0.602778 0 Td 46.450617 Tz (solution; ) Tj 51.428571 0 0 51.428571 0 1157.239157 Tm 6.358333 0 Td 50.636574 Tz (required ) Tj 2.586111 0 Td 58.796296 Tz (amounts ) Tj 2.663889 0 Td 58.333333 Tz (were ) Tj 1.575 0 Td 47.530864 Tz (for ) Tj 0.991667 0 Td 55.092593 Tz (each ) Tj 1.536111 0 Td 61.574074 Tz (mix ) Tj 1.283333 0 Td 58.333333 Tz (were ) Tj 1.575 0 Td 41.589506 Tz (filled ) Tj 1.652778 0 Td 46.180556 Tz (into ) Tj 1.302778 0 Td 42.12963 Tz (sterile ) Tj 1.944444 0 Td 55.092593 Tz (3 ) Tj 51.428571 0 0 51.428571 0 1117.216316 Tm 6.358333 0 Td 53.472222 Tz (cc ) Tj 0.816667 0 Td 49.826389 Tz (syringes ) Tj 2.566667 0 Td 51.851852 Tz (with ) Tj 1.438889 0 Td 51.851852 Tz (~1.5 ) Tj 1.438889 0 Td 61.574074 Tz (ml ) Tj 0.894444 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 60.358796 Tz (headroom ) Tj 3.052778 0 Td 59.41358 Tz (and ) Tj 1.205556 0 Td 41.589506 Tz (fitted ) Tj 1.652778 0 Td 52.662037 Tz (with ) Tj 1.419444 0 Td 51.851852 Tz (a ) Tj 0.486111 0 Td 42.12963 Tz (sterile ) Tj 51.428571 0 0 51.428571 0 1080.757282 Tm 6.377778 0 Td 51.388889 Tz (syringe ) Tj 2.294444 0 Td 36.111111 Tz (filter, ) Tj 1.711111 0 Td 50.771605 Tz (frozen ) Tj 2.022222 0 Td 53.652263 Tz (\(-80C.\), ) Tj 3.072222 0 Td 58.333333 Tz (and ) Tj 1.244444 0 Td 49.200337 Tz (lyophilized ) Tj 3.441667 0 Td 43.75 Tz (in ) Tj 0.7 0 Td 56.712963 Tz (an ) Tj 0.855556 0 Td 50.462963 Tz (upright ) Tj 51.428571 0 0 51.428571 0 1040.883212 Tm 6.358333 0 Td 46.450617 Tz (position. ) Tj 2.702778 0 Td 59.41358 Tz (Monocytes ) Tj 3.363889 0 Td 59.41358 Tz (and ) Tj 1.244444 0 Td 41.589506 Tz (nitric ) Tj 1.691667 0 Td 53.148148 Tz (oxide ) Tj 1.769444 0 Td 45.37037 Tz (in ) Tj 0.738889 0 Td 44.722222 Tz (vitro ) Tj 1.497222 0 Td 48.611111 Tz (assays: ) Tj 2.275 0 Td 60.493827 Tz (micros ) Tj 51.428571 0 0 51.428571 0 1002.601183 Tm 6.358333 0 Td 51.203704 Tz (copy, ) Tj 1.672222 0 Td 51.311728 Tz (Griess ) Tj 2.022222 0 Td 47.800926 Tz (reagent, ) Tj 2.430556 0 Td 58.333333 Tz (and ) Tj 1.186111 0 Td 63.194444 Tz (qRT-PCIn ) Tj 3.227778 0 Td 44.074074 Tz (vitro ) Tj 1.438889 0 Td 53.877315 Tz (temporal ) Tj 2.761111 0 Td 51.131687 Tz (induction ) Tj 51.428571 0 0 51.428571 0 962.693467 Tm 6.358333 0 Td 58.333333 Tz (of ) Tj 0.855556 0 Td 56.712963 Tz (an ) Tj 0.933333 0 Td 54.282407 Tz (inflammatory ) Tj 4.161111 0 Td 53.06713 Tz (response ) Tj 2.819444 0 Td 57.253086 Tz (due ) Tj 1.263889 0 Td 46.990741 Tz (to ) Tj 0.855556 0 Td 48.611111 Tz (material ) Tj 2.586111 0 Td 54.6875 Tz (exposure ) Tj 2.897222 0 Td 60.493827 Tz (was ) Tj 51.428571 0 0 51.428571 0 919.867725 Tm 6.358333 0 Td 51.446759 Tz (assessed ) Tj 2.858333 0 Td 53.148148 Tz (using ) Tj 1.963889 0 Td 49.691358 Tz (the ) Tj 1.225 0 Td 58.333333 Tz (TIB-71 ) Tj 2.566667 0 Td 68.055556 Tz (RAW264.7 ) Tj 3.616667 0 Td 44.560185 Tz (cell ) Tj 1.477778 0 Td 43.75 Tz (line ) Tj 1.477778 0 Td 62.654321 Tz (\(ATCC: ) Tj 51.428571 0 0 51.428571 0 879 Tm 6.358333 0 Td 55.812757 Tz (Manassas, ) Tj 3.422222 0 Td 44.722222 Tz (Va.\). ) Tj 1.827778 0 Td 51.851852 Tz (Culture ) Tj 2.644444 0 Td 57.87037 Tz (methods ) Tj 2.916667 0 Td 58.333333 Tz (were ) Tj 1.827778 0 Td 55.740741 Tz (based ) Tj 2.119444 0 Td 59.953704 Tz (on ) Tj 1.166667 0 Td 51.851852 Tz (those ) Tj 51.428571 0 0 51.428571 0 840.403101 Tm 6.358333 0 Td 51.851852 Tz (described ) Tj 3.013889 0 Td 63.194444 Tz (by ) Tj 1.011111 0 Td 51.388889 Tz (Nicolin ) Tj 2.372222 0 Td 45.37037 Tz (et ) Tj 0.777778 0 Td 38.888889 Tz (al. ) Tj 0.952778 0 Td 52.314815 Tz (Nicolin ) Tj 2.411111 0 Td 48.611111 Tz (V. ) Tj 0.836111 0 Td 49.907407 Tz (Ponti ) Tj 1.75 0 Td 56.712963 Tz (C, ) Tj 0.933333 0 Td 55.497685 Tz (Narducci ) Tj 2.916667 0 Td 38.888889 Tz (P. ) Tj 51.428571 0 0 51.428571 0 801.237449 Tm 6.358333 0 Td 44.722222 Tz (Grill ) Tj 1.613889 0 Td 50.231481 Tz (V. ) Tj 0.913889 0 Td 51.311728 Tz (Bortul ) Tj 2.158333 0 Td 50.231481 Tz (R. ) Tj 0.933333 0 Td 61.574074 Tz (Zweyer ) Tj 2.488889 0 Td 103.703704 Tz (M ) Tj 0.913889 0 Td 43.75 Tz (et ) Tj 0.797222 0 Td 38.888889 Tz (al. ) Tj 1.011111 0 Td 48.971193 Tz (Different ) Tj 2.916667 0 Td 45.910494 Tz (levels ) Tj 1.944444 0 Td 58.333333 Tz (of ) Tj 0.913889 0 Td 48.611111 Tz (the ) Tj 51.428571 0 0 51.428571 0 762.267327 Tm 6.358333 0 Td 53.06713 Tz (neuronal ) Tj 2.780556 0 Td 41.589506 Tz (nitric ) Tj 1.75 0 Td 53.148148 Tz (oxide ) Tj 1.827778 0 Td 51.851852 Tz (synthase ) Tj 2.702778 0 Td 53.240741 Tz (isoform ) Tj 2.508333 0 Td 55.902778 Tz (modulate ) Tj 2.877778 0 Td 50.771605 Tz (the ) Tj 1.127778 0 Td 44.560185 Tz (rate ) Tj 1.302778 0 Td 56.712963 Tz (of ) Tj 40 0 0 40 0 729 Tm 8.175 0 Td 60.416667 Tz (osteoclastic ) Tj 4.7 0 Td 58.611111 Tz (differentiation ) Tj 5.6 0 Td 75 Tz (of ) Tj 1.05 0 Td 73.611111 Tz (TIB-71 ) Tj 3.05 0 Td 75 Tz (and ) Tj 1.675 0 Td 82.8125 Tz (CRL-2278 ) Tj 4.325 0 Td 115.277778 Tz (RAW ) Tj 40 0 0 40 0 690 Tm 8.175 0 Td 70 Tz (264.7 ) Tj 2.375 0 Td 72.916667 Tz (murine ) Tj 2.85 0 Td 56.25 Tz (cell ) Tj 1.6 0 Td 61.309524 Tz (clones. ) Tj 2.8 0 Td 77.083333 Tz (Anat ) Tj 2.125 0 Td 93.75 Tz (RecA ) Tj 2.55 0 Td 70.138889 Tz (Discov ) Tj 2.85 0 Td 84.722222 Tz (Mol ) Tj 1.725 0 Td 65.625 Tz (Cell ) Tj 1.875 0 Td 70.833333 Tz (Evol ) Tj 51.428571 0 0 51.428571 0 642 Tm 6.358333 0 Td 47.962963 Tz (Biol. ) Tj 1.575 0 Td 54.444444 Tz (2005; ) Tj 1.788889 0 Td 50 Tz (286\(2\): ) Tj 2.177778 0 Td 52.314815 Tz (945-54. ) Tj 2.333333 0 Td 63.734568 Tz (The ) Tj 1.283333 0 Td 53.030303 Tz (transformed ) Tj 3.655556 0 Td 46.180556 Tz (line ) Tj 1.244444 0 Td 61.574074 Tz (was ) Tj 1.283333 0 Td 51.388889 Tz (derived ) Tj 40 0 0 40 0 611 Tm 8.175 0 Td 75 Tz (from ) Tj 2.375 0 Td 72.916667 Tz (an ) Tj 1.425 0 Td 73.809524 Tz (Abelson ) Tj 3.7 0 Td 71.354167 Tz (leukemia ) Tj 4 0 Td 66.025641 Tz (virus-induced ) Tj 5.7 0 Td 75.833333 Tz (tumor ) Tj 2.825 0 Td 58.333333 Tz (in ) Tj 1.3 0 Td 63.888889 Tz (the ) Tj 51.428571 0 0 51.428571 0 567.224076 Tm 6.377778 0 Td 57.407407 Tz (BALEB/c ) Tj 2.625 0 Td 43.75 Tz (strain ) Tj 1.769444 0 Td 59.41358 Tz (and ) Tj 1.244444 0 Td 47.800926 Tz (exhibits ) Tj 2.469444 0 Td 58.333333 Tz (a ) Tj 0.563889 0 Td 57.651072 Tz (monocyte/macrophage ) Tj 6.727778 0 Td 68.055556 Tz (pheno ) Tj 51.428571 0 0 51.428571 0 527.943662 Tm 6.338889 0 Td 53.472222 Tz (type ) Tj 1.438889 0 Td 53.472222 Tz (with ) Tj 1.477778 0 Td 49.826389 Tz (receptor ) Tj 2.508333 0 Td 47.530864 Tz (activator ) Tj 2.702778 0 Td 55.092593 Tz (of ) Tj 0.758333 0 Td 51.851852 Tz (nuclear ) Tj 2.313889 0 Td 47.530864 Tz (factor ) Tj 1.827778 0 Td 53.472222 Tz (kappa-B-ligand ) Tj 51.428571 0 0 51.428571 0 485.313013 Tm 6.377778 0 Td 61.358025 Tz (\(RANKL\)-induced ) Tj 5.794444 0 Td 46.882716 Tz (osteoclast-like ) Tj 4.511111 0 Td 45.802469 Tz (differentiation ) Tj 4.394444 0 Td 45.37037 Tz (potential. ) Tj 51.428571 0 0 51.428571 0 445 Tm 6.358333 0 Td 51.851852 Tz (All ) Tj 1.030556 0 Td 47.800926 Tz (cultures ) Tj 2.45 0 Td 58.333333 Tz (were ) Tj 1.516667 0 Td 53.796296 Tz (maintained ) Tj 3.325 0 Td 45.37037 Tz (in ) Tj 0.758333 0 Td 68.055556 Tz (10% ) Tj 1.380556 0 Td 46.666667 Tz (\(v/v\) ) Tj 1.497222 0 Td 42.777778 Tz (fetal ) Tj 1.419444 0 Td 54.552469 Tz (bovine ) Tj 2.061111 0 Td 67.407407 Tz (serum ) Tj 51.428571 0 0 51.428571 0 408.894869 Tm 6.377778 0 Td 56.17284 Tz (\(FBS-\) ) Tj 2.411111 0 Td 55.902778 Tz (supplemented ) Tj 4.375 0 Td 54.282407 Tz (high ) Tj 1.691667 0 Td 53.240741 Tz (glucose ) Tj 2.644444 0 Td 54.444444 Tz (Dulbecco's ) Tj 3.675 0 Td 55.902778 Tz (Modified ) Tj 51.428571 0 0 51.428571 0 369.382746 Tm 6.377778 0 Td 51.388889 Tz (Eagle's ) Tj 2.411111 0 Td 67.515432 Tz (Medium ) Tj 2.702778 0 Td 74.537037 Tz (\(DMEM-HG\) ) Tj 4.238889 0 Td 51.527778 Tz (containing ) Tj 3.363889 0 Td 59.41358 Tz (100 ) Tj 1.283333 0 Td 57.037037 Tz (IU/ml ) Tj 1.925 0 Td 63.194444 Tz (peni ) Tj 51.428571 0 0 51.428571 0 331.614035 Tm 6.358333 0 Td 39.814815 Tz (cillin, ) Tj 1.963889 0 Td 57.253086 Tz (100 ) Tj 1.302778 0 Td 54.444444 Tz (ug/ml ) Tj 1.847222 0 Td 51.103989 Tz (Streptomycin, ) Tj 4.2 0 Td 58.333333 Tz (and ) Tj 1.283333 0 Td 48.611111 Tz (2.5 ) Tj 1.419444 0 Td 57.523148 Tz (g/ml ) Tj 1.594444 0 Td 54.012346 Tz (amphotericin ) Tj 51.428571 0 0 51.428571 0 291.305455 Tm 6.377778 0 Td 51.851852 Tz (B. ) Tj 0.797222 0 Td 49.907407 Tz (Cells ) Tj 1.672222 0 Td 59.143519 Tz (were ) Tj 1.555556 0 Td 57.928241 Tz (expanded ) Tj 2.936111 0 Td 45.37037 Tz (in ) Tj 0.719444 0 Td 46.990741 Tz (T-flasks ) Tj 2.411111 0 Td 59.41358 Tz (and ) Tj 1.225 0 Td 54.6875 Tz (passaged ) Tj 2.761111 0 Td 63.194444 Tz (by ) Tj 0.913889 0 Td 51.446759 Tz (scraping ) Tj 51.428571 0 0 51.428571 0 249.908163 Tm 6.358333 0 Td 44.074074 Tz (cells ) Tj 1.555556 0 Td 52.932099 Tz (off ) Tj 1.069444 0 Td 50.771605 Tz (the ) Tj 1.127778 0 Td 46.018519 Tz (flask ) Tj 1.613889 0 Td 53.472222 Tz (with ) Tj 1.516667 0 Td 51.851852 Tz (a ) Tj 0.602778 0 Td 52.391975 Tz (rubber ) Tj 2.1 0 Td 55.452675 Tz (policeman ) Tj 3.227778 0 Td 60.493827 Tz (and ) Tj 1.341667 0 Td 47.170782 Tz (replating ) Tj 2.761111 0 Td 46.990741 Tz (at ) Tj 57.142857 0 0 57.142857 0 210.05364 Tm 5.7225 0 Td 53.666667 Tz (4x10" ) Tj 1.8725 0 Td 47.962963 Tz (cells/cm. ) Tj 2.905 0 Td 48.854167 Tz (Experimental ) Tj 3.7975 0 Td 43.020833 Tz (cultures ) Tj 2.3625 0 Td 52.5 Tz (of ) Tj 0.8925 0 Td 45.694444 Tz (10 ) Tj 1.1375 0 Td 39.666667 Tz (cells ) Tj 1.4875 0 Td 53.229167 Tz (were ) Tj 51.428571 0 0 51.428571 0 168.99115 Tm 6.377778 0 Td 54.552469 Tz (seeded ) Tj 2.138889 0 Td 52.662037 Tz (onto ) Tj 1.477778 0 Td 59.953704 Tz (60 ) Tj 0.894444 0 Td 90.740741 Tz (mm ) Tj 1.283333 0 Td 46.450617 Tz (tissue ) Tj 1.827778 0 Td 48.148148 Tz (culture ) Tj 2.177778 0 Td 45.37037 Tz (plastic ) Tj 2.119444 0 Td 44.074074 Tz (Petri ) Tj 1.497222 0 Td 50.231481 Tz (dishes ) Tj 2.022222 0 Td 43.75 Tz (in ) Tj 0.7 0 Td 58.333333 Tz (5 ) Tj 0.563889 0 Td 61.574074 Tz (ml ) Tj 30 0 0 30 0 2547 Tm 41.666667 0 Td 75 Tz (10 ) Tj 30 0 0 30 0 2339 Tm 41.666667 0 Td 72.222222 Tz (15 ) Tj 30 0 0 30 0 1923 Tm 41.6 0 Td 77.777778 Tz (25 ) Tj 30 0 0 30 0 1716 Tm 41.6 0 Td 80.555556 Tz (30 ) Tj 30 0 0 30 0 1507 Tm 41.6 0 Td 77.777778 Tz (35 ) Tj 30 0 0 30 0 1299 Tm 41.566667 0 Td 83.333333 Tz (40 ) Tj 30 0 0 30 0 1091 Tm 41.566667 0 Td 80.555556 Tz (45 ) Tj 30 0 0 30 0 883 Tm 41.6 0 Td 80.555556 Tz (50 ) Tj 30 0 0 30 0 675 Tm 41.6 0 Td 77.777778 Tz (55 ) Tj 30 0 0 30 0 467 Tm 41.633333 0 Td 77.777778 Tz (60 ) Tj 30 0 0 30 0 259 Tm 41.633333 0 Td 75 Tz (65 ) Tj 51.428571 0 0 51.428571 0 2969 Tm 33.541667 0 Td 79.398148 Tz (24 ) Tj 51.428571 0 0 51.428571 0 2915.839329 Tm 25.297222 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 65.354938 Tz (medium ) Tj 2.508333 0 Td 59.41358 Tz (and ) Tj 1.205556 0 Td 54.62963 Tz (allowed ) Tj 2.430556 0 Td 45.37037 Tz (to ) Tj 0.680556 0 Td 48.611111 Tz (attach ) Tj 1.886111 0 Td 49.259259 Tz (overnight. ) Tj 3.111111 0 Td 72.916667 Tz (On ) Tj 1.05 0 Td 49.691358 Tz (the ) Tj 1.030556 0 Td 51.851852 Tz (following ) Tj 51.428571 0 0 51.428571 0 2876.103286 Tm 25.297222 0 Td 48.611111 Tz (day, ) Tj 1.341667 0 Td 49.200337 Tz (Petridishes ) Tj 3.363889 0 Td 59.143519 Tz (were ) Tj 1.516667 0 Td 57.928241 Tz (randomly ) Tj 2.877778 0 Td 53.06713 Tz (assigned ) Tj 2.625 0 Td 46.990741 Tz (to ) Tj 0.719444 0 Td 57.253086 Tz (one ) Tj 1.127778 0 Td 50.231481 Tz (offive ) Tj 1.886111 0 Td 55.632716 Tz (groups ) Tj 51.428571 0 0 51.428571 0 2831.926115 Tm 25.316667 0 Td 51.041667 Tz (\(n=9/group\): ) Tj 4.063889 0 Td 43.75 Tz (1\) ) Tj 0.836111 0 Td 47.962963 Tz (untreated, ) Tj 3.15 0 Td 55.092593 Tz (time ) Tj 1.613889 0 Td 51.041667 Tz (Zero ) Tj 1.516667 0 Td 50.555556 Tz (basal ) Tj 1.808333 0 Td 48.611111 Tz (condition: ) Tj 3.247222 0 Td 50.231481 Tz (2-4\) ) Tj 51.428571 0 0 51.428571 0 2791.60105 Tm 25.297222 0 Td 54.282407 Tz (exposure ) Tj 2.838889 0 Td 45.37037 Tz (to ) Tj 0.816667 0 Td 47.530864 Tz (0.5 ) Tj 1.147222 0 Td 59.953704 Tz (ml ) Tj 0.952778 0 Td 48.37963 Tz (cylinders/dish ) Tj 4.297222 0 Td 59.953704 Tz (of ) Tj 0.855556 0 Td 55.092593 Tz (F1+, ) Tj 1.575 0 Td 55.092593 Tz (F2+, ) Tj 1.555556 0 Td 53.472222 Tz (or ) Tj 0.875 0 Td 62.654321 Tz (F2- ) Tj 1.361111 0 Td 48.611111 Tz (5\) ) Tj 0.836111 0 Td 51.851852 Tz (5 ) Tj 51.428571 0 0 51.428571 0 2751.679058 Tm 25.355556 0 Td 54.444444 Tz (ug/ml ) Tj 1.925 0 Td 50.771605 Tz (lipopolysaccharide ) Tj 5.755556 0 Td 58.333333 Tz (\(LPS\) ) Tj 2.022222 0 Td 57.253086 Tz (\(Simga ) Tj 2.372222 0 Td 61.574074 Tz (P/N ) Tj 1.4 0 Td 59.41358 Tz (L4516\) ) Tj 2.411111 0 Td 42.12963 Tz (nitric ) Tj 51.428571 0 0 51.428571 0 2707.970414 Tm 25.297222 0 Td 53.796296 Tz (oxide ) Tj 1.808333 0 Td 64.00463 Tz (\(NO\) ) Tj 1.711111 0 Td 54.228395 Tz (proinflammatory ) Tj 5.075 0 Td 53.292181 Tz (endotoxin ) Tj 3.072222 0 Td 46.585648 Tz (control. ) Tj 51.428571 0 0 51.428571 0 2666.756219 Tm 26.016667 0 Td 46.990741 Tz (Nitric ) Tj 1.847222 0 Td 54.444444 Tz (oxide ) Tj 1.808333 0 Td 64.00463 Tz (\(NO\) ) Tj 1.652778 0 Td 52.824074 Tz (production ) Tj 3.325 0 Td 62.654321 Tz (was ) Tj 1.263889 0 Td 51.851852 Tz (assessed ) Tj 2.663889 0 Td 52.777778 Tz (through ) Tj 2.430556 0 Td 58.981481 Tz (media ) Tj 40 0 0 40 0 2630 Tm 32.525 0 Td 53.571429 Tz (nitrite ) Tj 2.55 0 Td 55.952381 Tz (levels. ) Tj 2.725 0 Td 67.5 Tz (Basal ) Tj 2.4 0 Td 73.958333 Tz (measurements ) Tj 5.625 0 Td 76.041667 Tz (were ) Tj 2.125 0 Td 67.5 Tz (taken ) Tj 2.325 0 Td 64.583333 Tz (as ) Tj 1.1 0 Td 70.833333 Tz (time ) Tj 2 0 Td 65.625 Tz (Zero ) Tj 51.428571 0 0 51.428571 0 2581.936743 Tm 25.316667 0 Td 51.203704 Tz (\(n=9; ) Tj 1.827778 0 Td 56.388889 Tz (group ) Tj 2.041667 0 Td 45.37037 Tz (1\) ) Tj 0.797222 0 Td 59.41358 Tz (and ) Tj 1.322222 0 Td 46.666667 Tz (after ) Tj 1.633333 0 Td 42.12963 Tz (5, ) Tj 0.816667 0 Td 49.691358 Tz (24, ) Tj 1.147222 0 Td 59.41358 Tz (and ) Tj 1.361111 0 Td 56.712963 Tz (48 ) Tj 0.991667 0 Td 53.148148 Tz (hours ) Tj 1.886111 0 Td 58.333333 Tz (of ) Tj 0.875 0 Td 51.131687 Tz (treatment ) Tj 51.428571 0 0 51.428571 0 2544.521264 Tm 25.297222 0 Td 54.6875 Tz (exposure ) Tj 2.858333 0 Td 52.932099 Tz (\(n=3/group/time ) Tj 4.977778 0 Td 46.990741 Tz (point; ) Tj 1.963889 0 Td 54.552469 Tz (groups ) Tj 2.197222 0 Td 46.018519 Tz (2-5\). ) Tj 1.633333 0 Td 62.222222 Tz (Media ) Tj 2.138889 0 Td 40.740741 Tz (nitrite ) Tj 51.428571 0 0 51.428571 0 2500.816121 Tm 25.297222 0 Td 45.910494 Tz (levels ) Tj 1.944444 0 Td 59.953704 Tz (were ) Tj 1.730556 0 Td 51.041667 Tz (resolved ) Tj 2.722222 0 Td 52.5 Tz (using ) Tj 1.866667 0 Td 49.691358 Tz (the ) Tj 1.166667 0 Td 69.675926 Tz (GRIESS ) Tj 2.838889 0 Td 79.62963 Tz (REAGENT ) Tj 3.616667 0 Td 87.5 Tz (SYS ) Tj 51.428571 0 0 51.428571 0 2462.723404 Tm 25.297222 0 Td 85.339506 Tz (TEM ) Tj 1.672222 0 Td 58.738426 Tz (\(Promega ) Tj 2.975 0 Td 50.231481 Tz (Corp.; ) Tj 1.983333 0 Td 56.712963 Tz (Madison, ) Tj 2.858333 0 Td 52.5 Tz (Wis.\) ) Tj 1.691667 0 Td 54.012346 Tz (per ) Tj 1.088889 0 Td 49.691358 Tz (the ) Tj 1.069444 0 Td 52.849003 Tz (manufacture's ) Tj 51.428571 0 0 51.428571 0 2424.418968 Tm 25.297222 0 Td 47.890947 Tz (protocol. ) Tj 2.780556 0 Td 49.259259 Tz (Individual ) Tj 3.111111 0 Td 57.79321 Tz (sample ) Tj 2.255556 0 Td 50.231481 Tz (values ) Tj 1.963889 0 Td 59.143519 Tz (were ) Tj 1.555556 0 Td 53.148148 Tz (taken ) Tj 1.75 0 Td 50.231481 Tz (as ) Tj 0.758333 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 53.703704 Tz (average ) Tj 51.428571 0 0 51.428571 0 2385.626506 Tm 25.297222 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 50.05144 Tz (duplicate ) Tj 2.916667 0 Td 52.572016 Tz (measures. ) Tj 3.052778 0 Td 58.333333 Tz (Upstream ) Tj 3.033333 0 Td 55.092593 Tz (changes ) Tj 2.527778 0 Td 45.37037 Tz (in ) Tj 0.758333 0 Td 50.411523 Tz (inducible ) Tj 2.897222 0 Td 50.555556 Tz (\(type ) Tj 51.428571 0 0 51.428571 0 2345.449102 Tm 25.316667 0 Td 44.290123 Tz (IP-nitric ) Tj 2.586111 0 Td 53.796296 Tz (oxide ) Tj 1.788889 0 Td 51.851852 Tz (synthase ) Tj 2.663889 0 Td 59.953704 Tz (\(iNOS\) ) Tj 2.313889 0 Td 57.523148 Tz (gene ) Tj 1.536111 0 Td 52.175926 Tz (expression ) Tj 3.286111 0 Td 58.333333 Tz (were ) Tj 1.594444 0 Td 55.740741 Tz (deter ) Tj 51.428571 0 0 51.428571 0 2303.675889 Tm 25.297222 0 Td 60.925926 Tz (mined ) Tj 2.138889 0 Td 58.333333 Tz (on ) Tj 1.05 0 Td 46.843434 Tz (duplicates. ) Tj 3.480556 0 Td 84.259259 Tz (mRNA ) Tj 2.372222 0 Td 60.493827 Tz (was ) Tj 1.438889 0 Td 46.585648 Tz (isolated ) Tj 2.566667 0 Td 57.523148 Tz (from ) Tj 1.711111 0 Td 44.074074 Tz (cells ) Tj 1.633333 0 Td 60.493827 Tz (and ) Tj 51.428571 0 0 51.428571 0 2268.804124 Tm 25.316667 0 Td 50.771605 Tz (subjected ) Tj 3.033333 0 Td 43.75 Tz (to ) Tj 0.836111 0 Td 66.203704 Tz (qRT-PCR ) Tj 3.052778 0 Td 46.990741 Tz (to ) Tj 0.875 0 Td 46.843434 Tz (investigate ) Tj 3.363889 0 Td 55.555556 Tz (changes ) Tj 2.644444 0 Td 45.37037 Tz (in ) Tj 0.836111 0 Td 48.611111 Tz (the ) Tj 1.147222 0 Td 68.055556 Tz (iNOS ) Tj 51.428571 0 0 51.428571 0 2228.893168 Tm 25.297222 0 Td 57.523148 Tz (gene ) Tj 1.672222 0 Td 49.200337 Tz (expression. ) Tj 3.616667 0 Td 54.79798 Tz (Immediately ) Tj 3.927778 0 Td 45.37037 Tz (after ) Tj 1.633333 0 Td 54.768519 Tz (performing ) Tj 3.558333 0 Td 50.771605 Tz (the ) Tj 1.205556 0 Td 51.311728 Tz (Griess ) Tj 51.428571 0 0 51.428571 0 2184.856089 Tm 25.297222 0 Td 46.990741 Tz (assay, ) Tj 1.944444 0 Td 58.333333 Tz (media ) Tj 1.925 0 Td 59.41358 Tz (and ) Tj 1.283333 0 Td 48.971193 Tz (materials ) Tj 2.858333 0 Td 59.143519 Tz (were ) Tj 1.594444 0 Td 51.851852 Tz (discarded ) Tj 3.013889 0 Td 57.523148 Tz (from ) Tj 1.613889 0 Td 47.222222 Tz (dishes, ) Tj 2.177778 0 Td 59.41358 Tz (and ) Tj 51.428571 0 0 51.428571 0 2149.696815 Tm 25.297222 0 Td 56.388889 Tz (monolayers ) Tj 3.85 0 Td 58.333333 Tz (were ) Tj 1.788889 0 Td 50.771605 Tz (gently ) Tj 2.313889 0 Td 48.070988 Tz (rinsed ) Tj 2.177778 0 Td 52.662037 Tz (with ) Tj 1.691667 0 Td 52.391975 Tz (phosphate-buffered ) Tj 51.428571 0 0 51.428571 0 2106.326683 Tm 25.316667 0 Td 46.990741 Tz (saline ) Tj 1.847222 0 Td 59.62963 Tz (\(PBS\) ) Tj 1.963889 0 Td 58.333333 Tz (and ) Tj 1.225 0 Td 53.877315 Tz (scrapped ) Tj 2.761111 0 Td 46.180556 Tz (into ) Tj 1.302778 0 Td 49.691358 Tz (the ) Tj 1.088889 0 Td 42.669753 Tz (rinse. ) Tj 1.769444 0 Td 55.092593 Tz (For ) Tj 1.147222 0 Td 54.282407 Tz (time ) Tj 1.477778 0 Td 51.041667 Tz (Zero ) Tj 1.438889 0 Td 78.858025 Tz (mea ) Tj 51.428571 0 0 51.428571 0 2067.476658 Tm 25.316667 0 Td 45.37037 Tz (sures, ) Tj 1.847222 0 Td 44.722222 Tz (cells ) Tj 1.555556 0 Td 48.611111 Tz (for ) Tj 1.05 0 Td 55.092593 Tz (each ) Tj 1.536111 0 Td 67.12963 Tz (qRT-PRC ) Tj 2.994444 0 Td 57.79321 Tz (sample ) Tj 2.275 0 Td 50.411523 Tz (condition ) Tj 2.955556 0 Td 54.444444 Tz (\(n=2\) ) Tj 1.847222 0 Td 58.333333 Tz (were ) Tj 51.428571 0 0 51.428571 0 2029.551313 Tm 25.297222 0 Td 52.256944 Tz (obtained ) Tj 2.644444 0 Td 57.523148 Tz (from ) Tj 1.555556 0 Td 49.691358 Tz (the ) Tj 0.991667 0 Td 51.557239 Tz (populations ) Tj 3.558333 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 51.851852 Tz (four ) Tj 1.361111 0 Td 49.691358 Tz (dishes ) Tj 1.925 0 Td 59.953704 Tz (by ) Tj 0.875 0 Td 57.613169 Tz (combining ) Tj 3.247222 0 Td 48.611111 Tz (in ) Tj 51.428571 0 0 51.428571 0 1988.285714 Tm 25.297222 0 Td 55.092593 Tz (a ) Tj 0.622222 0 Td 51.851852 Tz (15 ) Tj 0.875 0 Td 59.953704 Tz (ml ) Tj 0.991667 0 Td 49.537037 Tz (conical ) Tj 2.294444 0 Td 47.962963 Tz (tube, ) Tj 1.652778 0 Td 59.41358 Tz (and ) Tj 1.283333 0 Td 48.611111 Tz (for ) Tj 1.05 0 Td 39.969136 Tz (all ) Tj 0.972222 0 Td 49.907407 Tz (other ) Tj 1.672222 0 Td 55.902778 Tz (time ) Tj 1.536111 0 Td 46.296296 Tz (points, ) Tj 2.177778 0 Td 55.555556 Tz (Samples ) Tj 51.428571 0 0 51.428571 0 1949.019324 Tm 25.316667 0 Td 55.902778 Tz (\(n=2 ) Tj 1.497222 0 Td 52.932099 Tz (per ) Tj 1.088889 0 Td 49.907407 Tz (condition\) ) Tj 3.15 0 Td 58.333333 Tz (were ) Tj 1.536111 0 Td 52.662037 Tz (obtained ) Tj 2.644444 0 Td 61.574074 Tz (by ) Tj 0.913889 0 Td 43.930041 Tz (splitting ) Tj 2.527778 0 Td 44.074074 Tz (cells ) Tj 1.477778 0 Td 57.523148 Tz (from ) Tj 1.555556 0 Td 59.41358 Tz (one ) Tj 51.428571 0 0 51.428571 0 1908.460154 Tm 25.297222 0 Td 58.333333 Tz (of ) Tj 0.875 0 Td 48.611111 Tz (the ) Tj 1.127778 0 Td 48.611111 Tz (three ) Tj 1.730556 0 Td 46.018519 Tz (replicates ) Tj 3.013889 0 Td 57.87037 Tz (between ) Tj 2.683333 0 Td 49.691358 Tz (the ) Tj 1.147222 0 Td 58.333333 Tz (two ) Tj 1.341667 0 Td 49.537037 Tz (conical ) Tj 2.372222 0 Td 46.450617 Tz (tubes. ) Tj 1.963889 0 Td 50.231481 Tz (Cell ) Tj 51.428571 0 0 51.428571 0 1873.204461 Tm 25.297222 0 Td 44.907407 Tz (pellets ) Tj 2.177778 0 Td 58.333333 Tz (were ) Tj 1.633333 0 Td 58.873457 Tz (formed ) Tj 2.333333 0 Td 61.574074 Tz (by ) Tj 1.030556 0 Td 46.882716 Tz (centrifugation, ) Tj 4.491667 0 Td 59.41358 Tz (and ) Tj 1.302778 0 Td 49.691358 Tz (the ) Tj 1.147222 0 Td 50.968013 Tz (Supernatant ) Tj 51.428571 0 0 51.428571 0 1831.419924 Tm 25.277778 0 Td 62.654321 Tz (was ) Tj 1.4 0 Td 46.342593 Tz (aspirated. ) Tj 3.033333 0 Td 50.555556 Tz (Cells ) Tj 1.769444 0 Td 59.143519 Tz (were ) Tj 1.652778 0 Td 51.851852 Tz (then ) Tj 1.516667 0 Td 50.231481 Tz (rinsed ) Tj 2.041667 0 Td 51.851852 Tz (again ) Tj 1.808333 0 Td 53.703704 Tz (through ) Tj 2.547222 0 Td 57.037037 Tz (resus ) Tj 51.428571 0 0 51.428571 0 1792.90559 Tm 25.297222 0 Td 56.018519 Tz (pending ) Tj 2.547222 0 Td 45.37037 Tz (in ) Tj 0.777778 0 Td 58.333333 Tz (PBS. ) Tj 1.613889 0 Td 49.907407 Tz (Cells ) Tj 1.711111 0 Td 58.333333 Tz (were ) Tj 1.594444 0 Td 51.041667 Tz (then ) Tj 1.419444 0 Td 48.611111 Tz (centrifuged, ) Tj 3.675 0 Td 59.41358 Tz (and ) Tj 1.263889 0 Td 48.611111 Tz (the ) Tj 1.069444 0 Td 59.62963 Tz (super ) Tj 51.428571 0 0 51.428571 0 1751.950898 Tm 25.297222 0 Td 49.691358 Tz (natant ) Tj 1.925 0 Td 48.611111 Tz (aspirated ) Tj 2.761111 0 Td 50.968013 Tz (completely. ) Tj 3.577778 0 Td 44.907407 Tz (Pellets ) Tj 2.061111 0 Td 58.333333 Tz (were ) Tj 1.575 0 Td 48.611111 Tz (stored ) Tj 1.925 0 Td 50.231481 Tz (frozen ) Tj 2.002778 0 Td 53.796296 Tz (\(-80 ) Tj 51.428571 0 0 51.428571 0 1712.982659 Tm 25.297222 0 Td 49.691358 Tz (C.\) ) Tj 1.108333 0 Td 43.425926 Tz (until ) Tj 1.477778 0 Td 55.092593 Tz (time ) Tj 1.497222 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 46.450617 Tz (analysis. ) Tj 51.428571 0 0 51.428571 0 1676.167353 Tm 26.036111 0 Td 66.435185 Tz (Upon ) Tj 1.983333 0 Td 47.962963 Tz (collecting ) Tj 3.208333 0 Td 60.493827 Tz (and ) Tj 1.458333 0 Td 49.826389 Tz (freezing ) Tj 2.741667 0 Td 41.049383 Tz (all ) Tj 1.108333 0 Td 51.388889 Tz (groups, ) Tj 2.527778 0 Td 45.37037 Tz (pellets ) Tj 2.294444 0 Td 59.143519 Tz (were ) Tj 52.857143 0 0 52.857143 0 1630.776331 Tm 24.594595 0 Td 57.282282 Tz (thawed ) Tj 2.175676 0 Td 57.807808 Tz (and ) Tj 1.172973 0 Td 80.930931 Tz (RNA ) Tj 1.57027 0 Td 60.960961 Tz (was ) Tj 1.248649 0 Td 46.114865 Tz (isolated ) Tj 2.308108 0 Td 51.711712 Tz (using ) Tj 1.683784 0 Td 47.297297 Tz (the ) Tj 1.002703 0 Td 78.303303 Tz (RNEASY ) Tj 2.951351 0 Td 68.918919 Tz (MINIKIT ) Tj 51.428571 0 0 51.428571 0 1592.485346 Tm 25.316667 0 Td 51.851852 Tz (\(Qiagen; ) Tj 2.644444 0 Td 60.395623 Tz (Germantown, ) Tj 4.122222 0 Td 60.763889 Tz (Md.\) ) Tj 1.575 0 Td 53.472222 Tz (with ) Tj 1.419444 0 Td 58.693416 Tz (on-column ) Tj 3.344444 0 Td 85.339506 Tz (DNA ) Tj 1.691667 0 Td 49.331276 Tz (digestion ) Tj 51.428571 0 0 51.428571 0 1553.640777 Tm 25.277778 0 Td 53.796296 Tz (using ) Tj 1.808333 0 Td 72.916667 Tz (RNASE-FREE ) Tj 4.588889 0 Td 79.722222 Tz (DNASE ) Tj 2.566667 0 Td 74.537037 Tz (SET ) Tj 1.516667 0 Td 50.771605 Tz (\(Qiagen\). ) Tj 2.994444 0 Td 83.179012 Tz (RNA ) Tj 1.711111 0 Td 46.990741 Tz (\(4.9 ) Tj 1.361111 0 Td 51.851852 Tz (ug\) ) Tj 58.571429 0 0 58.571429 0 1511.966844 Tm 22.195122 0 Td 55.01355 Tz (was ) Tj 1.280488 0 Td 43.089431 Tz (reverse ) Tj 2.065854 0 Td 43.200296 Tz (transcribed ) Tj 3.12439 0 Td 40.54878 Tz (into ) Tj 1.246341 0 Td 69.004065 Tz (cDNA ) Tj 1.929268 0 Td 46.097561 Tz (using ) Tj 1.639024 0 Td 41.734417 Tz (the ) Tj 1.07561 0 Td 61.178862 Tz (RT ) Tj 1.263415 0 Td 57.479675 Tz (FIRST ) Tj 51.428571 0 0 51.428571 0 1473.280385 Tm 25.316667 0 Td 80.478395 Tz (STRAND ) Tj 3.072222 0 Td 66.975309 Tz (KIT ) Tj 1.4 0 Td 53.877315 Tz (\(Qiagen\) ) Tj 2.761111 0 Td 58.333333 Tz (on ) Tj 0.894444 0 Td 55.092593 Tz (a ) Tj 0.505556 0 Td 81.28858 Tz (MASTERCYCLER ) Tj 6.027778 0 Td 69.675926 Tz (EP ) Tj 1.030556 0 Td 97.222222 Tz (GRA ) Tj 51.428571 0 0 51.428571 0 1437.804476 Tm 25.297222 0 Td 73.240741 Tz (DIENT ) Tj 2.294444 0 Td 52.73569 Tz (\(Eppendorf: ) Tj 3.694444 0 Td 58.738426 Tz (Hamburg, ) Tj 3.013889 0 Td 47.314815 Tz (Del.\) ) Tj 1.555556 0 Td 50.605413 Tz (thermocycler. ) Tj 4.083333 0 Td 72.106481 Tz (qPCR ) Tj 1.866667 0 Td 50.771605 Tz (\(25 ) Tj 51.428571 0 0 51.428571 0 1394.571965 Tm 25.355556 0 Td 42.12963 Tz (ul\) ) Tj 0.952778 0 Td 61.574074 Tz (was ) Tj 1.302778 0 Td 51.851852 Tz (then ) Tj 1.419444 0 Td 56.532922 Tz (performed ) Tj 3.247222 0 Td 59.953704 Tz (on ) Tj 0.894444 0 Td 58.333333 Tz (an ) Tj 0.933333 0 Td 73.611111 Tz (ICYCLER ) Tj 3.305556 0 Td 61.574074 Tz (IQ ) Tj 0.952778 0 Td 52.572016 Tz (\(Bio-Rad; ) Tj 3.111111 0 Td 71.296296 Tz (Her ) Tj 51.428571 0 0 51.428571 0 1354.56691 Tm 25.297222 0 Td 46.450617 Tz (cules, ) Tj 1.866667 0 Td 44.444444 Tz (Calif.\) ) Tj 2.041667 0 Td 48.611111 Tz (to ) Tj 0.777778 0 Td 54.012346 Tz (determine ) Tj 3.072222 0 Td 50.771605 Tz (threshold ) Tj 2.916667 0 Td 51.851852 Tz (cycle ) Tj 1.730556 0 Td 70.216049 Tz (\(C\) ) Tj 1.477778 0 Td 50.231481 Tz (values ) Tj 2.002778 0 Td 52.5 Tz (using ) Tj 51.428571 0 0 51.428571 0 1315.884131 Tm 25.375 0 Td 52.5 Tz (1.5ul ) Tj 1.769444 0 Td 58.333333 Tz (of ) Tj 0.797222 0 Td 55.092593 Tz (each ) Tj 1.497222 0 Td 79.398148 Tz (cDNA ) Tj 2.080556 0 Td 57.79321 Tz (sample ) Tj 2.275 0 Td 51.851852 Tz (using ) Tj 1.808333 0 Td 46.450617 Tz (1.5 ) Tj 1.069444 0 Td 43.75 Tz (ul ) Tj 0.7 0 Td 59.953704 Tz (of ) Tj 0.797222 0 Td 55.092593 Tz (each ) Tj 1.536111 0 Td 50.771605 Tz (\(sense ) Tj 2.002778 0 Td 59.41358 Tz (and ) Tj 51.428571 0 0 51.428571 0 1276.6171 Tm 25.297222 0 Td 48.021886 Tz (anti-sense\) ) Tj 3.461111 0 Td 56.712963 Tz (10 ) Tj 0.933333 0 Td 98.842593 Tz (mM ) Tj 1.419444 0 Td 55.092593 Tz (primer ) Tj 2.197222 0 Td 48.611111 Tz (solution ) Tj 2.625 0 Td 47.727273 Tz (\(Integrated ) Tj 3.422222 0 Td 85.339506 Tz (DNA ) Tj 1.75 0 Td 68.865741 Tz (Tech ) Tj 51.428571 0 0 51.428571 0 1236.356608 Tm 25.297222 0 Td 48.971193 Tz (nologies; ) Tj 2.877778 0 Td 46.843434 Tz (Coralville, ) Tj 3.344444 0 Td 54.444444 Tz (Iowa\) ) Tj 1.866667 0 Td 50.771605 Tz (\(Table ) Tj 2.041667 0 Td 42.12963 Tz (2\), ) Tj 1.030556 0 Td 50.231481 Tz (12.5 ) Tj 1.497222 0 Td 42.12963 Tz (ul ) Tj 0.719444 0 Td 79.398148 Tz (SYBR ) Tj 2.080556 0 Td 56.17284 Tz (Green/ ) Tj 51.428571 0 0 51.428571 0 1197.239609 Tm 25.316667 0 Td 50.968013 Tz (Fluorescein ) Tj 3.675 0 Td 71.296296 Tz (qPCR ) Tj 2.022222 0 Td 79.938272 Tz (MASTERMIX ) Tj 4.647222 0 Td 50.771605 Tz (\(Qiagen\), ) Tj 3.013889 0 Td 59.41358 Tz (and ) Tj 1.380556 0 Td 56.30787 Tz (nuclease ) Tj 51.428571 0 0 51.428571 0 1160.237624 Tm 25.297222 0 Td 46.990741 Tz (free ) Tj 1.361111 0 Td 47.530864 Tz (water. ) Tj 1.944444 0 Td 77.777778 Tz (cDNA ) Tj 2.080556 0 Td 53.347578 Tz (corresponding ) Tj 4.375 0 Td 45.37037 Tz (to ) Tj 0.797222 0 Td 57.253086 Tz (one ) Tj 1.225 0 Td 58.333333 Tz (gene ) Tj 1.594444 0 Td 61.574074 Tz (was ) Tj 1.341667 0 Td 51.131687 Tz (amplified ) Tj 51.428571 0 0 51.428571 0 1119.320388 Tm 25.297222 0 Td 52.932099 Tz (per ) Tj 1.127778 0 Td 46.666667 Tz (well, ) Tj 1.575 0 Td 59.41358 Tz (and ) Tj 1.205556 0 Td 79.398148 Tz (cDNA ) Tj 2.061111 0 Td 49.691358 Tz (for ) Tj 1.05 0 Td 67.24537 Tz (iNOS ) Tj 1.788889 0 Td 60.493827 Tz (and ) Tj 1.225 0 Td 55.902778 Tz (housekeeping ) Tj 4.161111 0 Td 55.740741 Tz (genes ) Tj 1.866667 0 Td 58.333333 Tz (ribo ) Tj 51.428571 0 0 51.428571 0 1078.373934 Tm 25.316667 0 Td 57.037037 Tz (somal ) Tj 1.925 0 Td 50.771605 Tz (18s ) Tj 1.069444 0 Td 60.493827 Tz (\(Rn ) Tj 1.166667 0 Td 49.421296 Tz (18s\) ) Tj 1.302778 0 Td 58.333333 Tz (and ) Tj 1.302778 0 Td 46.296296 Tz (B-actin ) Tj 2.080556 0 Td 77.12963 Tz (mRNAs ) Tj 2.469444 0 Td 59.143519 Tz (were ) Tj 1.536111 0 Td 50.771605 Tz (amplified ) Tj 2.897222 0 Td 48.611111 Tz (for ) Tj 0.972222 0 Td 39.969136 Tz (all ) Tj 51.428571 0 0 51.428571 0 1041.739241 Tm 25.316667 0 Td 51.851852 Tz (samples. ) Tj 2.819444 0 Td 48.611111 Tz (Additionally, ) Tj 4.161111 0 Td 46.990741 Tz (efficiency ) Tj 3.169444 0 Td 52.391975 Tz (curves ) Tj 2.255556 0 Td 58.333333 Tz (were ) Tj 1.75 0 Td 50 Tz (created ) Tj 2.45 0 Td 48.611111 Tz (for ) Tj 51.428571 0 0 51.428571 0 1000.308789 Tm 25.297222 0 Td 55.092593 Tz (each ) Tj 1.438889 0 Td 55.092593 Tz (primer ) Tj 2.061111 0 Td 48.611111 Tz (pair ) Tj 1.244444 0 Td 52.5 Tz (using ) Tj 1.691667 0 Td 49.691358 Tz (the ) Tj 1.011111 0 Td 43.209877 Tz (serial ) Tj 1.672222 0 Td 46.8107 Tz (dilutions ) Tj 2.663889 0 Td 53.472222 Tz (of ) Tj 0.719444 0 Td 55.092593 Tz (a ) Tj 0.447222 0 Td 69.675926 Tz (common ) Tj 2.663889 0 Td 77.777778 Tz (cDNA ) Tj 51.428571 0 0 51.428571 0 966 Tm 25.316667 0 Td 52.314815 Tz (sample. ) Tj 51.428571 0 0 51.428571 0 923.123077 Tm 26.036111 0 Td 55.295139 Tz (Photomicrographs ) Tj 5.541667 0 Td 58.333333 Tz (were ) Tj 1.613889 0 Td 52.256944 Tz (acquired ) Tj 2.702778 0 Td 43.75 Tz (at ) Tj 0.719444 0 Td 53.472222 Tz (40x. ) Tj 1.575 0 Td 53.796296 Tz (100x, ) Tj 1.827778 0 Td 58.333333 Tz (and ) Tj 1.263889 0 Td 61.574074 Tz (200x ) Tj 51.428571 0 0 51.428571 0 883.337349 Tm 25.297222 0 Td 49.691358 Tz (for ) Tj 0.972222 0 Td 39.969136 Tz (all ) Tj 0.855556 0 Td 54.552469 Tz (groups ) Tj 2.080556 0 Td 59.41358 Tz (and ) Tj 1.225 0 Td 52.662037 Tz (time ) Tj 1.380556 0 Td 49.151235 Tz (points ) Tj 1.925 0 Td 52.5 Tz (using ) Tj 1.672222 0 Td 56.712963 Tz (an ) Tj 0.894444 0 Td 74.537037 Tz (EVOS ) Tj 1.925 0 Td 81.018519 Tz (XL ) Tj 1.108333 0 Td 57.361111 Tz (Microscope ) Tj 51.428571 0 0 51.428571 0 843.486146 Tm 25.316667 0 Td 70 Tz (\(AMG; ) Tj 2.469444 0 Td 47.395833 Tz (Bothell, ) Tj 2.586111 0 Td 56.17284 Tz (Wash.\) ) Tj 2.333333 0 Td 45.37037 Tz (to ) Tj 0.875 0 Td 49.151235 Tz (assess ) Tj 2.119444 0 Td 54.095442 Tz (morphological ) Tj 4.530556 0 Td 52.256944 Tz (changes. ) Tj 51.428571 0 0 51.428571 0 802.891688 Tm 25.297222 0 Td 59.259259 Tz (Changes ) Tj 2.761111 0 Td 45.37037 Tz (in ) Tj 0.816667 0 Td 87.5 Tz (NO ) Tj 1.263889 0 Td 50.694444 Tz (concentrations ) Tj 4.530556 0 Td 58.333333 Tz (and ) Tj 1.302778 0 Td 68.055556 Tz (iNOS ) Tj 1.886111 0 Td 51.527778 Tz (expression ) Tj 3.344444 0 Td 50.231481 Tz (as ) Tj 0.855556 0 Td 59.953704 Tz (an ) Tj 51.428571 0 0 51.428571 0 761.856787 Tm 25.297222 0 Td 45.910494 Tz (effect ) Tj 1.886111 0 Td 55.092593 Tz (of ) Tj 0.797222 0 Td 51.131687 Tz (treatment ) Tj 2.975 0 Td 55.740741 Tz (group ) Tj 1.886111 0 Td 59.41358 Tz (and ) Tj 1.263889 0 Td 55.092593 Tz (time ) Tj 1.555556 0 Td 43.75 Tz (in ) Tj 0.777778 0 Td 48.148148 Tz (culture ) Tj 2.216667 0 Td 59.953704 Tz (were ) Tj 1.633333 0 Td 51.851852 Tz (assessed ) Tj 51.428571 0 0 51.428571 0 722.662864 Tm 25.277778 0 Td 53.796296 Tz (using ) Tj 1.905556 0 Td 74.537037 Tz (SAS ) Tj 1.633333 0 Td 80.208333 Tz (SOFTWARE ) Tj 4.141667 0 Td 65.625 Tz (\(SAS ) Tj 1.925 0 Td 42.453704 Tz (Institute; ) Tj 2.838889 0 Td 52.5 Tz (Cary, ) Tj 1.886111 0 Td 49.691358 Tz (N.C.\). ) Tj 2.1 0 Td 87.5 Tz (NO ) Tj 51.428571 0 0 51.428571 0 684.845144 Tm 25.297222 0 Td 50.462963 Tz (concentrations ) Tj 4.666667 0 Td 59.143519 Tz (were ) Tj 1.847222 0 Td 50.771605 Tz (subjected ) Tj 3.169444 0 Td 43.75 Tz (to ) Tj 0.991667 0 Td 41.319444 Tz (full ) Tj 1.438889 0 Td 43.930041 Tz (factorial ) Tj 2.838889 0 Td 60.185185 Tz (two-way ) Tj 51.428571 0 0 51.428571 0 645.103203 Tm 25.297222 0 Td 82.962963 Tz (ANOVA ) Tj 2.625 0 Td 55.092593 Tz (\(C-0.05\) ) Tj 2.8 0 Td 43.75 Tz (to ) Tj 0.680556 0 Td 48.611111 Tz (detect ) Tj 1.866667 0 Td 48.070988 Tz (factor ) Tj 1.866667 0 Td 48.070988 Tz (significance ) Tj 3.577778 0 Td 53.796296 Tz (using ) Tj 1.730556 0 Td 49.691358 Tz (the ) Tj 1.030556 0 Td 53.472222 Tz (proc ) Tj 51.428571 0 0 51.428571 0 606.979798 Tm 25.297222 0 Td 61.574074 Tz (glm ) Tj 1.4 0 Td 51.527778 Tz (procedure. ) Tj 3.422222 0 Td 51.311728 Tz (Factor ) Tj 2.119444 0 Td 45.910494 Tz (levels ) Tj 1.963889 0 Td 58.333333 Tz (were ) Tj 1.691667 0 Td 47.222222 Tz (further ) Tj 2.275 0 Td 48.070988 Tz (investigated ) Tj 3.772222 0 Td 47.530864 Tz (for ) Tj 51.428571 0 0 51.428571 0 564.741294 Tm 25.297222 0 Td 48.611111 Tz (differences ) Tj 3.5 0 Td 48.611111 Tz (\(C.-0.05\) ) Tj 2.877778 0 Td 52.5 Tz (using ) Tj 1.788889 0 Td 60.925926 Tz (Tukey ) Tj 2.138889 0 Td 78.858025 Tz (HSD ) Tj 1.672222 0 Td 56.388889 Tz (\(main ) Tj 1.983333 0 Td 45.775463 Tz (factors\) ) Tj 2.430556 0 Td 60.493827 Tz (and ) Tj 51.428571 0 0 51.428571 0 528.850764 Tm 25.297222 0 Td 59.683642 Tz (Tukey-Kramer ) Tj 4.433333 0 Td 46.450617 Tz (\(interaction ) Tj 3.538889 0 Td 45.37037 Tz (factor\) ) Tj 2.119444 0 Td 49.421296 Tz (multiple ) Tj 2.586111 0 Td 56.064815 Tz (comparison ) Tj 3.519444 0 Td 52.662037 Tz (test ) Tj 51.428571 0 0 51.428571 0 488.252163 Tm 25.297222 0 Td 50.771605 Tz (ing ) Tj 1.166667 0 Td 58.333333 Tz (of ) Tj 0.797222 0 Td 49.691358 Tz (the ) Tj 1.127778 0 Td 60.925926 Tz (means ) Tj 2.041667 0 Td 59.41358 Tz (and ) Tj 1.283333 0 Td 49.691358 Tz (the ) Tj 1.108333 0 Td 49.358974 Tz (least-squared ) Tj 4.102778 0 Td 55.092593 Tz (means, ) Tj 2.216667 0 Td 46.118234 Tz (respectively. ) Tj 51.428571 0 0 51.428571 0 447.11655 Tm 25.297222 0 Td 70.37037 Tz (QRT-PCR ) Tj 3.091667 0 Td 50.231481 Tz (data ) Tj 1.302778 0 Td 59.953704 Tz (were ) Tj 1.536111 0 Td 53.324916 Tz (transformed ) Tj 3.597222 0 Td 49.259259 Tz (prior ) Tj 1.575 0 Td 42.12963 Tz (to ) Tj 0.641667 0 Td 73.45679 Tz (ANOVA. ) Tj 2.780556 0 Td 48.935185 Tz (Efficiency ) Tj 51.428571 0 0 51.428571 0 407.017136 Tm 25.297222 0 Td 53.796296 Tz (curve ) Tj 1.788889 0 Td 49.421296 Tz (data ) Tj 1.4 0 Td 58.333333 Tz (were ) Tj 1.575 0 Td 55.092593 Tz (used ) Tj 1.497222 0 Td 45.37037 Tz (to ) Tj 0.758333 0 Td 53.652263 Tz (determine ) Tj 3.072222 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 50.636574 Tz (adjusted ) Tj 2.586111 0 Td 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49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 2006.300971 Tm 6.358333 0 Td 47.530864 Tz (vertebral ) Tj 2.741667 0 Td 49.074074 Tz (bodies. ) Tj 2.255556 0 Td 56.388889 Tz (Small ) Tj 1.866667 0 Td 54.282407 Tz (openings ) Tj 2.8 0 Td 58.333333 Tz (were ) Tj 1.575 0 Td 50 Tz (created ) Tj 2.275 0 Td 45.37037 Tz (in ) Tj 0.738889 0 Td 48.611111 Tz (the ) Tj 1.030556 0 Td 45.37037 Tz (cortical ) Tj 51.428571 0 0 51.428571 0 1968.603513 Tm 6.377778 0 Td 45.37037 Tz (shells ) Tj 1.866667 0 Td 54.095442 Tz (approximately ) Tj 4.55 0 Td 46.450617 Tz (1.5 ) Tj 1.108333 0 Td 90.740741 Tz (mm ) Tj 1.4 0 Td 42.669753 Tz (distal ) Tj 1.808333 0 Td 49.691358 Tz (the ) Tj 1.186111 0 Td 46.8107 Tz (vertebral ) Tj 2.761111 0 Td 51.131687 Tz (endplates ) Tj 51.428571 0 0 51.428571 0 1926.906077 Tm 6.338889 0 Td 53.148148 Tz (using ) Tj 1.925 0 Td 51.851852 Tz (a ) Tj 0.661111 0 Td 49.259259 Tz (tooth ) Tj 1.788889 0 Td 51.041667 Tz (burr ) Tj 1.516667 0 Td 58.333333 Tz (and ) Tj 1.380556 0 Td 38.348765 Tz (drill. ) Tj 1.672222 0 Td 87.5 Tz (A ) Tj 0.836111 0 Td 52.5 Tz (small ) Tj 1.886111 0 Td 61.574074 Tz (amount ) Tj 2.508333 0 Td 59.953704 Tz (of ) Tj 0.952778 0 Td 48.611111 Tz (the ) Tj 1.225 0 Td 62.654321 Tz (F1+ ) Tj 51.428571 0 0 51.428571 0 1890.015727 Tm 6.358333 0 Td 49.421296 Tz (material ) Tj 2.741667 0 Td 62.654321 Tz (was ) Tj 1.516667 0 Td 49.331276 Tz (delivered ) Tj 3.052778 0 Td 48.611111 Tz (via ) Tj 1.225 0 Td 46.8107 Tz (injection ) Tj 2.897222 0 Td 53.703704 Tz (through ) Tj 2.625 0 Td 58.333333 Tz (a ) Tj 0.758333 0 Td 55.497685 Tz (16-gauge ) Tj 51.428571 0 0 51.428571 0 1851.417437 Tm 6.358333 0 Td 49.074074 Tz (needle. ) Tj 2.469444 0 Td 58.333333 Tz (Using ) Tj 2.119444 0 Td 58.333333 Tz (a ) Tj 0.758333 0 Td 55.092593 Tz (micro ) Tj 2.080556 0 Td 59.548611 Tz (computed ) Tj 3.208333 0 Td 58.657407 Tz (tomography ) Tj 3.927778 0 Td 58.981481 Tz (\(LCT\) ) Tj 2.158333 0 Td 63.194444 Tz (40 ) Tj 51.428571 0 0 51.428571 0 1808.085575 Tm 6.377778 0 Td 54.62963 Tz (\(Scanco ) Tj 2.508333 0 Td 68.055556 Tz (USA, ) Tj 1.847222 0 Td 41.481481 Tz (Inc.; ) Tj 1.458333 0 Td 59.953704 Tz (Wayne, ) Tj 2.372222 0 Td 46.990741 Tz (Pa.\) ) Tj 1.283333 0 Td 58.333333 Tz (and ) Tj 1.225 0 Td 49.907407 Tz (associated ) Tj 3.188889 0 Td 52.662037 Tz (software ) Tj 2.683333 0 Td 72.916667 Tz (3D ) Tj 51.428571 0 0 51.428571 0 1770.191142 Tm 6.358333 0 Td 46.450617 Tz (reconstitution, ) Tj 4.297222 0 Td 48.611111 Tz (analysis ) Tj 2.469444 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 49.691358 Tz (the ) Tj 1.030556 0 Td 49.691358 Tz (vertebrae ) Tj 2.780556 0 Td 59.41358 Tz (and ) Tj 1.186111 0 Td 53.240741 Tz (implant ) Tj 2.333333 0 Td 48.611111 Tz (locations ) Tj 51.428571 0 0 51.428571 0 1733.109415 Tm 6.338889 0 Td 59.953704 Tz (were ) Tj 1.711111 0 Td 53.148148 Tz (performed. ) Tj 3.441667 0 Td 72.916667 Tz (An ) Tj 1.166667 0 Td 50.231481 Tz (area ) Tj 1.477778 0 Td 51.203704 Tz (containing ) Tj 3.363889 0 Td 52.662037 Tz (only ) Tj 1.536111 0 Td 50.771605 Tz (the ) Tj 1.166667 0 Td 53.240741 Tz (implant ) Tj 2.527778 0 Td 60.493827 Tz (was ) Tj 51.428571 0 0 51.428571 0 1693.176471 Tm 6.358333 0 Td 48.021886 Tz (partitioned ) Tj 3.383333 0 Td 60.493827 Tz (and ) Tj 1.302778 0 Td 50.771605 Tz (the ) Tj 1.186111 0 Td 59.953704 Tz (Volume ) Tj 2.391667 0 Td 51.388889 Tz (percent ) Tj 2.547222 0 Td 47.530864 Tz (B-tricalcium ) Tj 3.713889 0 Td 54.73251 Tz (phosphate ) Tj 51.428571 0 0 51.428571 0 1654.306329 Tm 6.358333 0 Td 58.333333 Tz (and ) Tj 1.516667 0 Td 48.070988 Tz (B-tricalcium ) Tj 3.830556 0 Td 54.73251 Tz (phosphate ) Tj 3.266667 0 Td 52.777778 Tz (granule ) Tj 2.566667 0 Td 53.472222 Tz (degree ) Tj 2.294444 0 Td 55.092593 Tz (of ) Tj 0.913889 0 Td 51.527778 Tz (anisotropy ) Tj 51.428571 0 0 51.428571 0 1611.06879 Tm 6.377778 0 Td 61.574074 Tz (\(DA\) ) Tj 1.769444 0 Td 61.574074 Tz (was ) Tj 1.419444 0 Td 49.826389 Tz (reported ) Tj 2.625 0 Td 61.574074 Tz (on ) Tj 1.05 0 Td 51.851852 Tz (using ) Tj 1.827778 0 Td 50.771605 Tz (the ) Tj 1.166667 0 Td 51.851852 Tz (software ) Tj 2.741667 0 Td 47.962963 Tz (functions. ) Tj 3.15 0 Td 57.037037 Tz (Three ) Tj 51.428571 0 0 51.428571 0 1573.076555 Tm 6.358333 0 Td 50.605413 Tz (implantations ) Tj 4.122222 0 Td 59.143519 Tz (were ) Tj 1.536111 0 Td 56.532922 Tz (performed ) Tj 3.188889 0 Td 59.41358 Tz (and ) Tj 1.244444 0 Td 51.851852 Tz (Scanned, ) Tj 2.644444 0 Td 51.851852 Tz (but ) Tj 1.069444 0 Td 53.472222 Tz (only ) Tj 1.477778 0 Td 57.253086 Tz (one ) Tj 1.166667 0 Td 42.939815 Tz (site ) Tj 51.428571 0 0 51.428571 0 1534.253416 Tm 6.338889 0 Td 62.654321 Tz (was ) Tj 1.4 0 Td 48.021886 Tz (partitioned ) Tj 3.422222 0 Td 51.851852 Tz (out ) Tj 1.166667 0 Td 49.691358 Tz (for ) Tj 1.088889 0 Td 50.771605 Tz (the ) Tj 1.283333 0 Td 48.070988 Tz (B-tricalcium ) Tj 3.733333 0 Td 54.73251 Tz (phosphate ) Tj 3.208333 0 Td 52.314815 Tz (granule ) Tj 51.428571 0 0 51.428571 0 1498 Tm 6.358333 0 Td 46.450617 Tz (analysis. ) Tj 51.428571 0 0 51.428571 0 1453.684211 Tm 7.097222 0 Td 51.041667 Tz (Cultures ) Tj 2.741667 0 Td 56.712963 Tz (from ) Tj 1.633333 0 Td 41.049383 Tz (all ) Tj 0.972222 0 Td 55.632716 Tz (groups ) Tj 2.275 0 Td 59.143519 Tz (were ) Tj 1.652778 0 Td 61.574074 Tz (composed ) Tj 3.188889 0 Td 61.574074 Tz (of ) Tj 0.894444 0 Td 44.074074 Tz (cells ) Tj 1.594444 0 Td 59.62963 Tz (which ) Tj 51.428571 0 0 51.428571 0 1415.499369 Tm 6.338889 0 Td 47.170782 Tz (typically ) Tj 2.819444 0 Td 50.411523 Tz (exhibited ) Tj 3.013889 0 Td 51.851852 Tz (a ) Tj 0.602778 0 Td 48.251029 Tz (spherical ) Tj 2.916667 0 Td 58.657407 Tz (morphology ) Tj 3.830556 0 Td 52.662037 Tz (with ) Tj 1.555556 0 Td 48.611111 Tz (the ) Tj 1.166667 0 Td 65.625 Tz (occa ) Tj 51.428571 0 0 51.428571 0 1374.855769 Tm 6.377778 0 Td 46.990741 Tz (sional ) Tj 1.925 0 Td 52.824074 Tz (occurrence ) Tj 3.363889 0 Td 56.712963 Tz (of ) Tj 0.777778 0 Td 42.939815 Tz (cell ) Tj 1.225 0 Td 48.125 Tz (spreading/flattening ) Tj 5.969444 0 Td 52.662037 Tz (onto ) Tj 1.458333 0 Td 49.691358 Tz (the ) Tj 1.069444 0 Td 46.450617 Tz (tissue ) Tj 51.428571 0 0 51.428571 0 1335.891381 Tm 6.358333 0 Td 48.148148 Tz (culture ) Tj 2.158333 0 Td 42.534722 Tz (plastic. ) Tj 2.216667 0 Td 63.734568 Tz (Fl+ ) Tj 1.263889 0 Td 58.333333 Tz (and ) Tj 1.244444 0 Td 60.133745 Tz (F2-groups ) Tj 3.383333 0 Td 54.282407 Tz (demonstrated ) Tj 4.025 0 Td 55.092593 Tz (a ) Tj 0.466667 0 Td 45.37037 Tz (relatively ) Tj 51.428571 0 0 51.428571 0 1296.613941 Tm 6.358333 0 Td 48.611111 Tz (consistent ) Tj 3.266667 0 Td 58.333333 Tz (morphology ) Tj 3.85 0 Td 43.75 Tz (at ) Tj 0.836111 0 Td 39.969136 Tz (all ) Tj 1.05 0 Td 54.282407 Tz (time ) Tj 1.613889 0 Td 49.151235 Tz (points ) Tj 2.1 0 Td 48.611111 Tz (as ) Tj 0.913889 0 Td 53.472222 Tz (only ) Tj 1.613889 0 Td 55.092593 Tz (a ) Tj 0.641667 0 Td 52.5 Tz (small ) Tj 51.428571 0 0 51.428571 0 1256.483568 Tm 6.358333 0 Td 49.537037 Tz (portion ) Tj 2.216667 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 48.611111 Tz (the ) Tj 0.991667 0 Td 43.425926 Tz (cells ) Tj 1.419444 0 Td 54.282407 Tz (demonstrated ) Tj 4.025 0 Td 50.231481 Tz (spreading, ) Tj 3.111111 0 Td 59.41358 Tz (and ) Tj 1.186111 0 Td 51.203704 Tz (those ) Tj 1.652778 0 Td 58.873457 Tz (spread ) Tj 51.428571 0 0 51.428571 0 1218.3663 Tm 6.358333 0 Td 50.771605 Tz (ing ) Tj 1.127778 0 Td 43.425926 Tz (cells ) Tj 1.458333 0 Td 46.8107 Tz (typically ) Tj 2.741667 0 Td 58.333333 Tz (had ) Tj 1.205556 0 Td 58.333333 Tz (a ) Tj 0.505556 0 Td 47.800926 Tz (spindle-like ) Tj 3.597222 0 Td 50.231481 Tz (shape. ) Tj 2.002778 0 Td 58.333333 Tz (These ) Tj 1.925 0 Td 56.388889 Tz (round ) Tj 1.847222 0 Td 60.493827 Tz (and ) Tj 51.428571 0 0 51.428571 0 1180.084507 Tm 6.377778 0 Td 50 Tz (spindle ) Tj 2.255556 0 Td 55.092593 Tz (morphologies ) Tj 4.122222 0 Td 58.333333 Tz (and ) Tj 1.166667 0 Td 51.851852 Tz (population ) Tj 3.266667 0 Td 51.851852 Tz (percentages ) Tj 3.577778 0 Td 59.143519 Tz (were ) Tj 1.555556 0 Td 62.384259 Tz (simi ) Tj 51.428571 0 0 51.428571 0 1137.724848 Tm 6.358333 0 Td 44.290123 Tz (lar ) Tj 0.913889 0 Td 45.37037 Tz (to ) Tj 0.719444 0 Td 48.611111 Tz (the ) Tj 0.991667 0 Td 50.591564 Tz (overnight-attached ) Tj 5.561111 0 Td 44.722222 Tz (cells ) Tj 1.497222 0 Td 43.75 Tz (at ) Tj 0.680556 0 Td 52.662037 Tz (time ) Tj 1.438889 0 Td 46.018519 Tz (zero. ) Tj 1.536111 0 Td 51.203704 Tz (After ) Tj 1.652778 0 Td 58.333333 Tz (48 ) Tj 0.855556 0 Td 53.796296 Tz (hours ) Tj 51.428571 0 0 51.428571 0 1099.40796 Tm 6.338889 0 Td 50.771605 Tz (the ) Tj 1.166667 0 Td 49.826389 Tz (majority ) Tj 2.644444 0 Td 58.333333 Tz (of ) Tj 0.816667 0 Td 43.425926 Tz (cells ) Tj 1.575 0 Td 35.648148 Tz (still ) Tj 1.302778 0 Td 50.05144 Tz (exhibited ) Tj 2.877778 0 Td 55.092593 Tz (a ) Tj 0.583333 0 Td 48.251029 Tz (spherical ) Tj 2.877778 0 Td 54.79798 Tz (morphology, ) Tj 51.428571 0 0 51.428571 0 1059.418546 Tm 6.338889 0 Td 52.932099 Tz (but ) Tj 1.127778 0 Td 44.074074 Tz (cells ) Tj 1.536111 0 Td 45.37037 Tz (in ) Tj 0.758333 0 Td 49.691358 Tz (the ) Tj 1.088889 0 Td 63.734568 Tz (F2+ ) Tj 1.322222 0 Td 60.493827 Tz (and ) Tj 1.302778 0 Td 66.975309 Tz (LPS ) Tj 1.4 0 Td 51.851852 Tz (groups, ) Tj 2.352778 0 Td 55.092593 Tz (appeared ) Tj 2.838889 0 Td 43.055556 Tz (larger. ) Tj 2.022222 0 Td 72.106481 Tz (Occa ) Tj 51.428571 0 0 51.428571 0 1017.315469 Tm 6.377778 0 Td 47.530864 Tz (sional ) Tj 1.847222 0 Td 44.722222 Tz (cells ) Tj 1.516667 0 Td 42.12963 Tz (in ) Tj 0.719444 0 Td 62.654321 Tz (F2+ ) Tj 1.263889 0 Td 60.493827 Tz (and ) Tj 1.244444 0 Td 66.975309 Tz (LPS ) Tj 1.361111 0 Td 47.800926 Tz (cultures ) Tj 2.45 0 Td 59.143519 Tz (were ) Tj 1.575 0 Td 43.75 Tz (irregular, ) Tj 2.8 0 Td 44.074074 Tz (flattened, ) Tj 51.428571 0 0 51.428571 0 980.711138 Tm 6.358333 0 Td 58.333333 Tz (and ) Tj 1.186111 0 Td 53.472222 Tz (spread ) Tj 2.061111 0 Td 52.662037 Tz (with ) Tj 1.438889 0 Td 62.384259 Tz (more ) Tj 1.633333 0 Td 47.530864 Tz (filopodia ) Tj 2.722222 0 Td 59.953704 Tz (compared ) Tj 3.033333 0 Td 42.12963 Tz (to ) Tj 0.680556 0 Td 49.691358 Tz (the ) Tj 1.069444 0 Td 62.654321 Tz (F1+ ) Tj 1.283333 0 Td 58.333333 Tz (and ) Tj 1.225 0 Td 93.981481 Tz (F2 ) Tj 40 0 0 40 0 945 Tm 8.175 0 Td 61.458333 Tz (cultures ) Tj 3.2 0 Td 75 Tz (and ) Tj 1.575 0 Td 64.166667 Tz (basal ) Tj 2.15 0 Td 69.791667 Tz (time ) Tj 1.925 0 Td 65.625 Tz (Zero ) Tj 1.825 0 Td 62.5 Tz (condition. ) Tj 51.428571 0 0 51.428571 0 899.372032 Tm 7.097222 0 Td 75.617284 Tz (Now ) Tj 1.672222 0 Td 46.8107 Tz (referring ) Tj 2.819444 0 Td 43.75 Tz (to ) Tj 0.836111 0 Td 54.282407 Tz (FIG. ) Tj 1.555556 0 Td 48.611111 Tz (9, ) Tj 0.894444 0 Td 57.253086 Tz (measurements ) Tj 4.375 0 Td 50.231481 Tz (associated ) Tj 3.286111 0 Td 54.282407 Tz (with ) Tj 51.428571 0 0 51.428571 0 861.83 Tm 6.358333 0 Td 87.5 Tz (NO ) Tj 1.322222 0 Td 52.175926 Tz (production ) Tj 3.441667 0 Td 57.523148 Tz (were ) Tj 1.691667 0 Td 54.12037 Tz (normalized ) Tj 3.519444 0 Td 49.074074 Tz (against ) Tj 2.313889 0 Td 50.771605 Tz (the ) Tj 1.166667 0 Td 53.703704 Tz (average ) Tj 2.488889 0 Td 50.555556 Tz (basal ) Tj 51.428571 0 0 51.428571 0 819 Tm 6.358333 0 Td 46.018519 Tz (level ) Tj 1.691667 0 Td 58.333333 Tz (of ) Tj 0.933333 0 Td 41.203704 Tz (nitrite ) Tj 2.022222 0 Td 45.37037 Tz (at ) Tj 0.875 0 Td 53.472222 Tz (time ) Tj 1.613889 0 Td 50.231481 Tz (zero ) Tj 1.555556 0 Td 50.636574 Tz (\(3.5+0.9 ) Tj 2.8 0 Td 57.523148 Tz (uM\). ) Tj 1.672222 0 Td 62.654321 Tz (The ) Tj 1.458333 0 Td 45.833333 Tz (effects ) Tj 2.236111 0 Td 58.333333 Tz (of ) Tj 48.571429 0 0 48.571429 0 781 Tm 6.711765 0 Td 51.813725 Tz (treatment, ) Tj 3.232353 0 Td 58.333333 Tz (time ) Tj 1.544118 0 Td 48.039216 Tz (in ) Tj 0.720588 0 Td 48.468137 Tz (culture, ) Tj 2.45 0 Td 62.908497 Tz (and ) Tj 1.276471 0 Td 49.59893 Tz (interaction ) Tj 3.417647 0 Td 57.47549 Tz (term ) Tj 1.564706 0 Td 61.764706 Tz (were ) Tj 1.585294 0 Td 43.464052 Tz (all ) Tj 0.947059 0 Td 60.392157 Tz (found ) Tj 51.428571 0 0 51.428571 0 743.194279 Tm 6.338889 0 Td 45.37037 Tz (to ) Tj 0.738889 0 Td 45.868946 Tz (significantly ) Tj 3.752778 0 Td 49.421296 Tz (increase ) Tj 2.547222 0 Td 85.87963 Tz (NO ) Tj 1.147222 0 Td 50.462963 Tz (concentrations ) Tj 4.413889 0 Td 54.372428 Tz (\(p<0.0001 ) Tj 3.169444 0 Td 48.611111 Tz (for ) Tj 0.972222 0 Td 41.049383 Tz (all ) Tj 51.428571 0 0 51.428571 0 702.023346 Tm 6.358333 0 Td 43.930041 Tz (factors\). ) Tj 2.663889 0 Td 56.712963 Tz (Exposure ) Tj 2.955556 0 Td 45.37037 Tz (to ) Tj 0.797222 0 Td 55.092593 Tz (F2+, ) Tj 1.594444 0 Td 55.092593 Tz (F2-, ) Tj 1.555556 0 Td 58.333333 Tz (and ) Tj 1.302778 0 Td 66.975309 Tz (LPS ) Tj 1.438889 0 Td 41.049383 Tz (all ) Tj 0.972222 0 Td 55.092593 Tz (induced ) Tj 2.527778 0 Td 55.092593 Tz (a ) Tj 0.583333 0 Td 59.41358 Tz (sig ) Tj 51.428571 0 0 51.428571 0 662.665463 Tm 6.358333 0 Td 45.775463 Tz (nificant ) Tj 2.313889 0 Td 54.822531 Tz (accumulation ) Tj 4.102778 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 87.5 Tz (NO ) Tj 1.186111 0 Td 59.953704 Tz (by ) Tj 0.894444 0 Td 45.37037 Tz (at ) Tj 0.680556 0 Td 45.37037 Tz (least ) Tj 1.497222 0 Td 59.953704 Tz (48 ) Tj 0.855556 0 Td 54.444444 Tz (hours ) Tj 1.808333 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 55.092593 Tz (exposure ) Tj 51.428571 0 0 51.428571 0 625.737218 Tm 6.377778 0 Td 52.824074 Tz (\(p<0.0001, ) Tj 3.363889 0 Td 47.962963 Tz (0.02, ) Tj 1.613889 0 Td 58.333333 Tz (and ) Tj 1.225 0 Td 51.388889 Tz (0.0001, ) Tj 2.372222 0 Td 45.833333 Tz (respectively\). ) Tj 4.005556 0 Td 55.497685 Tz (Averages ) Tj 2.838889 0 Td 56.17284 Tz (associ ) Tj 40 0 0 40 0 590 Tm 8.175 0 Td 65.625 Tz (ated ) Tj 1.85 0 Td 67.708333 Tz (with ) Tj 1.925 0 Td 80.555556 Tz (F2+ ) Tj 1.725 0 Td 76.388889 Tz (and ) Tj 1.65 0 Td 87.5 Tz (LPS ) Tj 1.875 0 Td 64.166667 Tz (treatments ) Tj 4.15 0 Td 69.047619 Tz (reached ) Tj 3.15 0 Td 62.5 Tz (ten ) Tj 1.4 0 Td 67.5 Tz (times ) Tj 2.3 0 Td 58.333333 Tz (that ) Tj 1.675 0 Td 72.916667 Tz (of ) Tj 51.428571 0 0 51.428571 0 543.255102 Tm 6.338889 0 Td 50.771605 Tz (the ) Tj 1.166667 0 Td 53.472222 Tz (time ) Tj 1.497222 0 Td 51.851852 Tz (Zero ) Tj 1.497222 0 Td 48.611111 Tz (basal ) Tj 1.75 0 Td 42.12963 Tz (level. ) Tj 1.769444 0 Td 51.446759 Tz (Cultures ) Tj 2.702778 0 Td 56.944444 Tz (exposed ) Tj 2.625 0 Td 43.75 Tz (to ) Tj 0.816667 0 Td 66.975309 Tz (LPS ) Tj 1.458333 0 Td 62.114198 Tz (showed ) Tj 51.428571 0 0 51.428571 0 505.230672 Tm 6.377778 0 Td 45.664983 Tz (significant ) Tj 3.305556 0 Td 54.282407 Tz (accumulation ) Tj 4.238889 0 Td 46.018519 Tz (after ) Tj 1.672222 0 Td 52.662037 Tz (only ) Tj 1.613889 0 Td 59.953704 Tz (24 ) Tj 1.030556 0 Td 53.148148 Tz (hours ) Tj 1.925 0 Td 56.712963 Tz (of ) Tj 0.894444 0 Td 51.49177 Tz (treatment ) Tj 51.428571 0 0 51.428571 0 467.171429 Tm 6.377778 0 Td 51.557239 Tz (\(p=0.0036\). ) Tj 3.558333 0 Td 56.712963 Tz (Although ) Tj 2.877778 0 Td 48.206019 Tz (cultures ) Tj 2.488889 0 Td 56.944444 Tz (exposed ) Tj 2.547222 0 Td 45.37037 Tz (to ) Tj 0.758333 0 Td 55.092593 Tz (F1 ) Tj 0.777778 0 Td 58.333333 Tz (+ ) Tj 0.525 0 Td 54.822531 Tz (demonstrated ) Tj 51.428571 0 0 51.428571 0 425.108434 Tm 6.358333 0 Td 56.712963 Tz (an ) Tj 0.836111 0 Td 53.240741 Tz (average ) Tj 2.372222 0 Td 55.681818 Tz (accumulated ) Tj 3.830556 0 Td 41.203704 Tz (nitrite ) Tj 1.866667 0 Td 46.018519 Tz (level ) Tj 1.497222 0 Td 53.796296 Tz (twice ) Tj 1.730556 0 Td 46.180556 Tz (that ) Tj 1.244444 0 Td 53.472222 Tz (of ) Tj 0.719444 0 Td 55.092593 Tz (time ) Tj 1.477778 0 Td 50.231481 Tz (Zero ) Tj 1.361111 0 Td 45.37037 Tz (at ) Tj 51.428571 0 0 51.428571 0 385.521472 Tm 6.358333 0 Td 61.574074 Tz (48 ) Tj 0.894444 0 Td 50.231481 Tz (hours, ) Tj 2.022222 0 Td 45.664983 Tz (significant ) Tj 3.208333 0 Td 48.611111 Tz (differences ) Tj 3.402778 0 Td 57.407407 Tz (between ) Tj 2.625 0 Td 56.712963 Tz (F1 ) Tj 0.758333 0 Td 61.574074 Tz (+ ) Tj 0.602778 0 Td 45.37037 Tz (levels ) Tj 1.808333 0 Td 60.493827 Tz (and ) Tj 1.263889 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 345.56917 Tm 6.338889 0 Td 49.907407 Tz (basal ) Tj 1.808333 0 Td 54.282407 Tz (time ) Tj 1.575 0 Td 51.041667 Tz (Zero ) Tj 1.497222 0 Td 51.131687 Tz (condition ) Tj 3.072222 0 Td 58.333333 Tz (were ) Tj 1.691667 0 Td 51.851852 Tz (not ) Tj 1.205556 0 Td 50.231481 Tz (detected ) Tj 2.683333 0 Td 46.990741 Tz (in ) Tj 0.836111 0 Td 58.333333 Tz (any ) Tj 1.341667 0 Td 59.953704 Tz (of ) Tj 0.894444 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 311.83871 Tm 6.358333 0 Td 50.636574 Tz (multiple ) Tj 2.741667 0 Td 55.387205 Tz (comparisons ) Tj 3.927778 0 Td 46.8107 Tz (analysis. ) Tj 2.819444 0 Td 56.712963 Tz (Although ) Tj 3.052778 0 Td 64.814815 Tz (F2- ) Tj 1.458333 0 Td 61.574074 Tz (was ) Tj 1.4 0 Td 49.074074 Tz (signifi ) Tj 51.428571 0 0 51.428571 0 266.86052 Tm 6.358333 0 Td 49.151235 Tz (cantly ) Tj 1.886111 0 Td 51.131687 Tz (increased ) Tj 2.877778 0 Td 53.472222 Tz (over ) Tj 1.361111 0 Td 46.180556 Tz (that ) Tj 1.225 0 Td 51.851852 Tz (of ) Tj 0.7 0 Td 48.611111 Tz (the ) Tj 1.011111 0 Td 48.611111 Tz (basal ) Tj 1.594444 0 Td 48.611111 Tz (condition, ) Tj 3.052778 0 Td 58.333333 Tz (no ) Tj 0.816667 0 Td 49.259259 Tz (difference ) Tj 40 0 0 40 0 234 Tm 8.175 0 Td 58.333333 Tz (in ) Tj 0.95 0 Td 69.791667 Tz (accumulation ) Tj 5.275 0 Td 80.555556 Tz (was ) Tj 1.725 0 Td 64.583333 Tz (detected ) Tj 3.3 0 Td 74.404762 Tz (between ) Tj 3.4 0 Td 41.666667 Tz (it ) Tj 0.725 0 Td 75 Tz (and ) Tj 1.6 0 Td 62.5 Tz (the ) Tj 1.4 0 Td 70.833333 Tz (F1 ) Tj 0.975 0 Td 79.166667 Tz (+ ) Tj 0.7 0 Td 80 Tz (condi ) Tj 51.428571 0 0 51.428571 0 191.81602 Tm 6.338889 0 Td 47.800926 Tz (tion ) Tj 1.361111 0 Td 43.75 Tz (at ) Tj 0.7 0 Td 58.333333 Tz (any ) Tj 1.244444 0 Td 55.092593 Tz (time ) Tj 1.516667 0 Td 45.910494 Tz (point. ) Tj 1.866667 0 Td 63.734568 Tz (The ) Tj 1.322222 0 Td 49.016204 Tz (analysis ) Tj 2.586111 0 Td 52.932099 Tz (Suggested ) Tj 3.033333 0 Td 46.990741 Tz (that ) Tj 1.283333 0 Td 50.771605 Tz (the ) Tj 1.127778 0 Td 59.953704 Tz (pres ) Tj 30 0 0 30 0 2547 Tm 41.666667 0 Td 75 Tz (10 ) Tj 30 0 0 30 0 2339 Tm 41.666667 0 Td 72.222222 Tz (15 ) Tj 30 0 0 30 0 1923 Tm 41.6 0 Td 77.777778 Tz (25 ) Tj 30 0 0 30 0 1716 Tm 41.6 0 Td 80.555556 Tz (30 ) Tj 30 0 0 30 0 1507 Tm 41.6 0 Td 77.777778 Tz (35 ) Tj 30 0 0 30 0 1299 Tm 41.566667 0 Td 83.333333 Tz (40 ) Tj 30 0 0 30 0 1091 Tm 41.566667 0 Td 80.555556 Tz (45 ) Tj 30 0 0 30 0 883 Tm 41.6 0 Td 80.555556 Tz (50 ) Tj 30 0 0 30 0 675 Tm 41.6 0 Td 77.777778 Tz (55 ) Tj 30 0 0 30 0 467 Tm 41.633333 0 Td 77.777778 Tz (60 ) Tj 30 0 0 30 0 259 Tm 41.633333 0 Td 75 Tz (65 ) Tj 52.857143 0 0 52.857143 0 2968 Tm 32.635135 0 Td 77.252252 Tz (26 ) Tj 51.428571 0 0 51.428571 0 2915.438662 Tm 25.297222 0 Td 55.902778 Tz (ence ) Tj 1.555556 0 Td 59.953704 Tz (of ) Tj 0.855556 0 Td 72.106481 Tz (hCPR ) Tj 1.963889 0 Td 51.49177 Tz (bioagents ) Tj 3.033333 0 Td 45.37037 Tz (in ) Tj 0.777778 0 Td 55.092593 Tz (combination ) Tj 3.908333 0 Td 52.662037 Tz (with ) Tj 1.536111 0 Td 55.740741 Tz (lower ) Tj 1.847222 0 Td 55.632716 Tz (weight ) Tj 51.428571 0 0 51.428571 0 2876.935129 Tm 25.297222 0 Td 51.388889 Tz (percent ) Tj 2.391667 0 Td 48.206019 Tz (alginate ) Tj 2.547222 0 Td 52.932099 Tz (and/or ) Tj 2.1 0 Td 52.932099 Tz (higher ) Tj 2.158333 0 Td 59.953704 Tz (Volume ) Tj 2.391667 0 Td 51.851852 Tz (percentages ) Tj 3.713889 0 Td 56.712963 Tz (of ) Tj 0.816667 0 Td 74.537037 Tz (TCP ) Tj 51.428571 0 0 51.428571 0 2833.101449 Tm 25.316667 0 Td 54.444444 Tz (\(F1+\) ) Tj 1.808333 0 Td 52.824074 Tz (maintained ) Tj 3.325 0 Td 51.851852 Tz (a ) Tj 0.447222 0 Td 49.907407 Tz (basal ) Tj 1.633333 0 Td 87.5 Tz (NO ) Tj 1.166667 0 Td 52.5 Tz (production ) Tj 3.325 0 Td 44.560185 Tz (rate ) Tj 1.205556 0 Td 45.37037 Tz (in ) Tj 0.738889 0 Td 57.253086 Tz (monocytes ) Tj 3.227778 0 Td 48.611111 Tz (in ) Tj 51.428571 0 0 51.428571 0 2790.494297 Tm 25.297222 0 Td 56.388889 Tz (comparison ) Tj 3.558333 0 Td 46.990741 Tz (to ) Tj 0.777778 0 Td 45.37037 Tz (that ) Tj 1.283333 0 Td 52.662037 Tz (seen ) Tj 1.438889 0 Td 50.231481 Tz (associated ) Tj 3.227778 0 Td 51.851852 Tz (with ) Tj 1.458333 0 Td 58.333333 Tz (5 ) Tj 0.836111 0 Td 58.333333 Tz (g/ml ) Tj 1.633333 0 Td 65.895062 Tz (LPS ) Tj 1.4 0 Td 61.574074 Tz (endot ) Tj 51.428571 0 0 51.428571 0 2748.321909 Tm 25.297222 0 Td 53.472222 Tz (oxin ) Tj 1.477778 0 Td 54.6875 Tz (exposure ) Tj 2.780556 0 Td 54.282407 Tz (\(and ) Tj 1.516667 0 Td 50.555556 Tz (F2+\). ) Tj 1.730556 0 Td 55.902778 Tz (Whereas, ) Tj 2.858333 0 Td 54.552469 Tz (accumulation ) Tj 4.122222 0 Td 45.37037 Tz (in ) Tj 0.758333 0 Td 48.611111 Tz (the ) Tj 1.088889 0 Td 62.654321 Tz (F2+ ) Tj 51.428571 0 0 51.428571 0 2710.079667 Tm 25.297222 0 Td 56.388889 Tz (group ) Tj 1.886111 0 Td 58.873457 Tz (\(hCPR, ) Tj 2.275 0 Td 52.932099 Tz (higher ) Tj 2.041667 0 Td 55.092593 Tz (weight ) Tj 2.119444 0 Td 50.028935 Tz (percentalginate, ) Tj 4.958333 0 Td 59.41358 Tz (and ) Tj 1.244444 0 Td 55.092593 Tz (lower ) Tj 1.808333 0 Td 62.654321 Tz (Vol ) Tj 51.428571 0 0 51.428571 0 2667.191919 Tm 25.277778 0 Td 71.296296 Tz (ume ) Tj 1.594444 0 Td 52.824074 Tz (percentage ) Tj 3.5 0 Td 56.712963 Tz (of ) Tj 1.127778 0 Td 46.990741 Tz (3-tricalcium ) Tj 3.694444 0 Td 55.092593 Tz (phosphate ) Tj 3.325 0 Td 53.703704 Tz (reached ) Tj 2.586111 0 Td 45.910494 Tz (levels ) Tj 51.428571 0 0 51.428571 0 2624.517986 Tm 25.297222 0 Td 56.712963 Tz (comparable ) Tj 3.577778 0 Td 45.37037 Tz (to ) Tj 0.758333 0 Td 45.37037 Tz (that ) Tj 1.263889 0 Td 58.333333 Tz (of ) Tj 0.797222 0 Td 68.055556 Tz (LPS ) Tj 1.419444 0 Td 45.37037 Tz (after ) Tj 1.536111 0 Td 58.333333 Tz (48 ) Tj 0.875 0 Td 49.691358 Tz (hours. ) Tj 51.428571 0 0 51.428571 0 2585.1181 Tm 26.016667 0 Td 49.608262 Tz (Transcription ) Tj 4.063889 0 Td 59.953704 Tz (of ) Tj 0.797222 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 68.865741 Tz (iNOS ) Tj 1.847222 0 Td 57.523148 Tz (gene ) Tj 1.575 0 Td 43.75 Tz (in ) Tj 0.738889 0 Td 53.06713 Tz (response ) Tj 2.722222 0 Td 45.37037 Tz (to ) Tj 0.738889 0 Td 49.691358 Tz (the ) Tj 1.088889 0 Td 50 Tz (various ) Tj 51.428571 0 0 51.428571 0 2539.766864 Tm 25.277778 0 Td 50.231481 Tz (treatments ) Tj 3.208333 0 Td 58.333333 Tz (and ) Tj 1.205556 0 Td 48.611111 Tz (culture ) Tj 2.138889 0 Td 53.148148 Tz (times ) Tj 1.75 0 Td 61.574074 Tz (was ) Tj 1.302778 0 Td 59.259259 Tz (modeled ) Tj 2.644444 0 Td 52.5 Tz (using ) Tj 1.711111 0 Td 53.914141 Tz (transformed ) Tj 51.428571 0 0 51.428571 0 2500.629454 Tm 25.297222 0 Td 66.666667 Tz (qRT-PCR ) Tj 2.936111 0 Td 44.722222 Tz (data. ) Tj 1.536111 0 Td 55.902778 Tz (Data ) Tj 1.477778 0 Td 50.05144 Tz (reduction ) Tj 2.838889 0 Td 62.654321 Tz (was ) Tj 1.244444 0 Td 56.532922 Tz (performed ) Tj 3.169444 0 Td 52.5 Tz (using ) Tj 1.691667 0 Td 47.530864 Tz (the ) Tj 1.011111 0 Td 65.625 Tz (AACt ) Tj 51.428571 0 0 51.428571 0 2459.777778 Tm 25.297222 0 Td 60.493827 Tz (method ) Tj 2.488889 0 Td 51.851852 Tz (\(Ct ) Tj 1.225 0 Td 47.890947 Tz (indicates ) Tj 2.877778 0 Td 55.092593 Tz (primer ) Tj 2.236111 0 Td 47.314815 Tz (efficiency ) Tj 3.169444 0 Td 50.771605 Tz (\(Table ) Tj 2.138889 0 Td 50.231481 Tz (4\) ) Tj 0.913889 0 Td 51.041667 Tz (adjusted ) Tj 51.428571 0 0 51.428571 0 2414.784906 Tm 25.277778 0 Td 50.771605 Tz (threshold ) Tj 2.936111 0 Td 50.555556 Tz (cycle ) Tj 1.730556 0 Td 50.231481 Tz (values ) Tj 2.041667 0 Td 58.333333 Tz (were ) Tj 1.594444 0 Td 52.5 Tz (used\) ) Tj 1.788889 0 Td 53.472222 Tz (with ) Tj 1.536111 0 Td 64.814815 Tz (Rn ) Tj 0.855556 0 Td 52.932099 Tz (18s ) Tj 1.166667 0 Td 48.611111 Tz (as ) Tj 0.816667 0 Td 48.611111 Tz (the ) Tj 1.069444 0 Td 64.814815 Tz (house ) Tj 51.428571 0 0 51.428571 0 2377.228717 Tm 25.297222 0 Td 54.62963 Tz (keeping ) Tj 2.586111 0 Td 51.203704 Tz (gene. ) Tj 1.886111 0 Td 57.685185 Tz (Given ) Tj 2.022222 0 Td 51.851852 Tz (the ) Tj 1.225 0 Td 47.962963 Tz (large ) Tj 1.75 0 Td 46.990741 Tz (efficiency ) Tj 3.169444 0 Td 51.203704 Tz (value ) Tj 1.827778 0 Td 50.231481 Tz (associated ) Tj 51.428571 0 0 51.428571 0 2333.479167 Tm 25.277778 0 Td 54.282407 Tz (with ) Tj 1.555556 0 Td 51.851852 Tz (the ) Tj 1.302778 0 Td 45.833333 Tz (B-actin ) Tj 2.216667 0 Td 54.552469 Tz (primer ) Tj 2.197222 0 Td 42.777778 Tz (pair, ) Tj 1.575 0 Td 50.231481 Tz (data ) Tj 1.477778 0 Td 49.907407 Tz (associated ) Tj 3.266667 0 Td 52.662037 Tz (with ) Tj 1.555556 0 Td 37.808642 Tz (its ) Tj 0.972222 0 Td 57.523148 Tz (gene ) Tj 51.428571 0 0 51.428571 0 2293.427136 Tm 25.297222 0 Td 51.527778 Tz (expression ) Tj 3.383333 0 Td 59.143519 Tz (were ) Tj 1.691667 0 Td 51.851852 Tz (taken ) Tj 1.847222 0 Td 50.231481 Tz (as ) Tj 0.875 0 Td 53.292181 Tz (erroneous ) Tj 3.15 0 Td 59.41358 Tz (and ) Tj 1.341667 0 Td 49.151235 Tz (disregarded. ) Tj 3.869444 0 Td 55.092593 Tz (Fold ) Tj 51.428571 0 0 51.428571 0 2252.009732 Tm 25.297222 0 Td 57.253086 Tz (change ) Tj 2.236111 0 Td 50.231481 Tz (values ) Tj 2.002778 0 Td 43.75 Tz (in ) Tj 0.719444 0 Td 85.069444 Tz (mRNA ) Tj 2.197222 0 Td 51.527778 Tz (expression ) Tj 3.247222 0 Td 50.231481 Tz (as ) Tj 0.777778 0 Td 59.548611 Tz (compared ) Tj 3.013889 0 Td 46.990741 Tz (to ) Tj 0.719444 0 Td 50.771605 Tz (the ) Tj 1.030556 0 Td 50.555556 Tz (basal ) Tj 51.428571 0 0 51.428571 0 2208.172702 Tm 25.277778 0 Td 55.092593 Tz (time ) Tj 1.652778 0 Td 51.041667 Tz (Zero ) Tj 1.555556 0 Td 51.527778 Tz (expression ) Tj 3.461111 0 Td 44.074074 Tz (level ) Tj 1.672222 0 Td 50.771605 Tz (are ) Tj 1.244444 0 Td 61.574074 Tz (shown ) Tj 2.216667 0 Td 43.75 Tz (in ) Tj 0.894444 0 Td 54.282407 Tz (FIG. ) Tj 1.672222 0 Td 47.530864 Tz (10. ) Tj 1.186111 0 Td 71.296296 Tz (An ) Tj 1.205556 0 Td 58.333333 Tz (up ) Tj 51.428571 0 0 51.428571 0 2167.950064 Tm 25.297222 0 Td 48.935185 Tz (regulation ) Tj 3.169444 0 Td 59.953704 Tz (of ) Tj 0.855556 0 Td 67.24537 Tz (iNOS ) Tj 1.886111 0 Td 60.493827 Tz (was ) Tj 1.322222 0 Td 53.472222 Tz (seen ) Tj 1.536111 0 Td 48.611111 Tz (for ) Tj 1.069444 0 Td 39.969136 Tz (all ) Tj 0.972222 0 Td 50.771605 Tz (treatment ) Tj 2.955556 0 Td 54.552469 Tz (groups ) Tj 2.216667 0 Td 58.333333 Tz (by ) Tj 0.952778 0 Td 45.37037 Tz (at ) Tj 51.428571 0 0 51.428571 0 2125.764706 Tm 25.297222 0 Td 44.722222 Tz (least ) Tj 1.516667 0 Td 58.333333 Tz (24 ) Tj 0.894444 0 Td 49.151235 Tz (hours, ) Tj 1.983333 0 Td 49.691358 Tz (the ) Tj 1.069444 0 Td 54.282407 Tz (time ) Tj 1.438889 0 Td 51.203704 Tz (point ) Tj 1.730556 0 Td 57.685185 Tz (which ) Tj 1.905556 0 Td 54.822531 Tz (corresponded ) Tj 4.122222 0 Td 53.472222 Tz (with ) Tj 1.458333 0 Td 56.712963 Tz (each ) Tj 51.428571 0 0 51.428571 0 2083.540313 Tm 25.277778 0 Td 50.084175 Tz (treatment's ) Tj 3.538889 0 Td 69.444444 Tz (maximum ) Tj 3.15 0 Td 57.928241 Tz (measured ) Tj 2.975 0 Td 51.851852 Tz (expression ) Tj 3.325 0 Td 43.209877 Tz (level. ) Tj 1.827778 0 Td 58.333333 Tz (LPS, ) Tj 1.594444 0 Td 60.493827 Tz (and ) Tj 51.428571 0 0 51.428571 0 2043.205847 Tm 25.297222 0 Td 50.231481 Tz (possibly ) Tj 2.663889 0 Td 54.282407 Tz (F2-, ) Tj 1.497222 0 Td 48.881173 Tz (treatment\(s\) ) Tj 3.752778 0 Td 54.62963 Tz (induced ) Tj 2.469444 0 Td 58.333333 Tz (an ) Tj 0.913889 0 Td 47.962963 Tz (early ) Tj 1.672222 0 Td 53.06713 Tz (response ) Tj 2.741667 0 Td 72.916667 Tz (show ) Tj 51.428571 0 0 51.428571 0 2002.247312 Tm 25.297222 0 Td 51.851852 Tz (ing ) Tj 1.341667 0 Td 51.131687 Tz (increased ) Tj 3.169444 0 Td 47.115385 Tz (transcription ) Tj 4.102778 0 Td 45.37037 Tz (after ) Tj 1.75 0 Td 52.662037 Tz (only ) Tj 1.75 0 Td 43.75 Tz (five ) Tj 1.458333 0 Td 49.691358 Tz (hours. ) Tj 2.236111 0 Td 68.055556 Tz (iNOS ) Tj 51.428571 0 0 51.428571 0 1959.51018 Tm 25.297222 0 Td 51.851852 Tz (expression ) Tj 3.208333 0 Td 46.450617 Tz (levels ) Tj 1.788889 0 Td 40.509259 Tz (fell ) Tj 1.088889 0 Td 46.990741 Tz (in ) Tj 0.680556 0 Td 41.049383 Tz (all ) Tj 0.855556 0 Td 55.092593 Tz (groups ) Tj 2.1 0 Td 57.407407 Tz (between ) Tj 2.547222 0 Td 49.691358 Tz (the ) Tj 0.991667 0 Td 59.953704 Tz (24 ) Tj 0.855556 0 Td 58.333333 Tz (and ) Tj 1.166667 0 Td 61.574074 Tz (48 ) Tj 0.836111 0 Td 57.523148 Tz (hour ) Tj 51.428571 0 0 51.428571 0 1916.939686 Tm 25.277778 0 Td 55.092593 Tz (time ) Tj 1.458333 0 Td 46.296296 Tz (points. ) Tj 2.158333 0 Td 55.092593 Tz (F1+, ) Tj 1.516667 0 Td 55.092593 Tz (F2+, ) Tj 1.477778 0 Td 59.41358 Tz (and ) Tj 1.244444 0 Td 55.681818 Tz (F2-material ) Tj 3.85 0 Td 54.552469 Tz (groups ) Tj 2.119444 0 Td 59.41358 Tz (had ) Tj 1.244444 0 Td 50.636574 Tz (returned ) Tj 51.428571 0 0 51.428571 0 1877.224335 Tm 25.277778 0 Td 45.37037 Tz (to ) Tj 0.738889 0 Td 55.092593 Tz (or ) Tj 0.816667 0 Td 56.944444 Tz (dropped ) Tj 2.547222 0 Td 57.685185 Tz (below ) Tj 1.983333 0 Td 48.611111 Tz (the ) Tj 1.05 0 Td 49.259259 Tz (basal ) Tj 1.672222 0 Td 51.527778 Tz (expression ) Tj 3.305556 0 Td 45.37037 Tz (level ) Tj 1.555556 0 Td 58.333333 Tz (by ) Tj 0.913889 0 Td 59.953704 Tz (48 ) Tj 0.875 0 Td 49.691358 Tz (hours. ) Tj 51.428571 0 0 51.428571 0 1834.936446 Tm 26.036111 0 Td 57.928241 Tz (Response ) Tj 3.052778 0 Td 50 Tz (surface ) Tj 2.352778 0 Td 56.712963 Tz (modeling ) Tj 2.955556 0 Td 59.953704 Tz (of ) Tj 0.875 0 Td 49.691358 Tz (the ) Tj 1.147222 0 Td 47.962963 Tz (linearized ) Tj 3.130556 0 Td 61.574074 Tz (ACt ) Tj 1.419444 0 Td 49.691358 Tz (values ) Tj 51.428571 0 0 51.428571 0 1792.539216 Tm 25.297222 0 Td 49.331276 Tz (indicated ) Tj 2.877778 0 Td 46.254209 Tz (significant ) Tj 3.247222 0 Td 45.37037 Tz (effects ) Tj 2.138889 0 Td 56.712963 Tz (of ) Tj 0.797222 0 Td 41.049383 Tz (all ) Tj 0.972222 0 Td 46.296296 Tz (factors ) Tj 2.158333 0 Td 58.333333 Tz (and ) Tj 1.263889 0 Td 55.092593 Tz (accounted ) Tj 3.188889 0 Td 47.530864 Tz (for ) Tj 58.571429 0 0 58.571429 0 1749 Tm 22.229268 0 Td 63.550136 Tz (81% ) Tj 1.502439 0 Td 52.642276 Tz (of ) Tj 0.904878 0 Td 42.682927 Tz (the ) Tj 1.143902 0 Td 44.105691 Tz (variance ) Tj 2.47561 0 Td 47.235772 Tz (\(R-value\). ) Tj 3.158537 0 Td 56.910569 Tz (The ) Tj 1.34878 0 Td 43.315266 Tz (covariate ) Tj 2.697561 0 Td 43.394309 Tz (material ) Tj 51.428571 0 0 51.428571 0 1711.298246 Tm 25.297222 0 Td 54.372428 Tz (parameter ) Tj 3.208333 0 Td 54.282407 Tz (demonstrated ) Tj 4.161111 0 Td 55.092593 Tz (a ) Tj 0.622222 0 Td 48.070988 Tz (significance ) Tj 3.713889 0 Td 46.450617 Tz (effect ) Tj 1.963889 0 Td 43.75 Tz (in ) Tj 0.816667 0 Td 49.691358 Tz (the ) Tj 1.186111 0 Td 59.62963 Tz (model ) Tj 51.428571 0 0 51.428571 0 1669.453237 Tm 25.316667 0 Td 51.557239 Tz (\(p<0.0001\). ) Tj 3.538889 0 Td 63.194444 Tz (Time ) Tj 1.652778 0 Td 61.574074 Tz (was ) Tj 1.244444 0 Td 56.388889 Tz (found ) Tj 1.827778 0 Td 46.990741 Tz (to ) Tj 0.7 0 Td 46.118234 Tz (significantly ) Tj 3.733333 0 Td 45.910494 Tz (affect ) Tj 1.788889 0 Td 48.611111 Tz (the ) Tj 1.011111 0 Td 50.231481 Tz (data ) Tj 1.341667 0 Td 50.231481 Tz (as ) Tj 52.857143 0 0 52.857143 0 1624.499383 Tm 24.613514 0 Td 53.603604 Tz (a ) Tj 0.510811 0 Td 51.501502 Tz (second-order ) Tj 3.859459 0 Td 53.253253 Tz (parameter ) Tj 3.045946 0 Td 42.567568 Tz (in ) Tj 0.7 0 Td 57.387387 Tz (which ) Tj 1.92973 0 Td 52.027027 Tz (both ) Tj 1.418919 0 Td 49.399399 Tz (the ) Tj 1.097297 0 Td 44.66967 Tz (linear ) Tj 1.778378 0 Td 56.756757 Tz (and ) Tj 1.22973 0 Td 59.90991 Tz (more ) Tj 51.428571 0 0 51.428571 0 1587.997546 Tm 25.316667 0 Td 51.851852 Tz (so ) Tj 0.855556 0 Td 48.611111 Tz (the ) Tj 1.108333 0 Td 49.691358 Tz (quadratic ) Tj 2.897222 0 Td 53.148148 Tz (parameters ) Tj 3.441667 0 Td 58.333333 Tz (were ) Tj 1.613889 0 Td 45.959596 Tz (significant ) Tj 3.266667 0 Td 55.092593 Tz (\(p=0.0078 ) Tj 3.208333 0 Td 59.41358 Tz (and ) Tj 51.428571 0 0 51.428571 0 1544.726392 Tm 25.297222 0 Td 51.851852 Tz (0.0017, ) Tj 2.430556 0 Td 45.833333 Tz (respectively\). ) Tj 4.044444 0 Td 63.734568 Tz (The ) Tj 1.361111 0 Td 49.691358 Tz (quadratic ) Tj 2.897222 0 Td 54.73251 Tz (parameter ) Tj 3.130556 0 Td 48.611111 Tz (for ) Tj 1.069444 0 Td 53.472222 Tz (time ) Tj 1.458333 0 Td 60.493827 Tz (and ) Tj 40 0 0 40 0 1507 Tm 32.5 0 Td 65.277778 Tz (the ) Tj 1.625 0 Td 59.722222 Tz (linear ) Tj 2.575 0 Td 62.5 Tz (material ) Tj 3.425 0 Td 63.425926 Tz (covariate ) Tj 3.875 0 Td 75 Tz (were ) Tj 2.225 0 Td 76.041667 Tz (most ) Tj 2.225 0 Td 57.19697 Tz (influential ) Tj 4.225 0 Td 60.416667 Tz (in ) Tj 1.15 0 Td 65.277778 Tz (the ) Tj 51.428571 0 0 51.428571 0 1457 Tm 25.297222 0 Td 59.62963 Tz (model ) Tj 1.963889 0 Td 50.231481 Tz (as ) Tj 0.777778 0 Td 44.722222 Tz (their ) Tj 1.477778 0 Td 50.231481 Tz (values ) Tj 1.983333 0 Td 58.333333 Tz (were ) Tj 1.516667 0 Td 51.49177 Tz (estimated ) Tj 2.955556 0 Td 43.75 Tz (at ) Tj 0.661111 0 Td 55.632716 Tz (-0.375 ) Tj 2.177778 0 Td 59.41358 Tz (and ) Tj 1.225 0 Td 53.148148 Tz (0.448 ) Tj 1.769444 0 Td 39.699074 Tz (\(all ) Tj 51.428571 0 0 51.428571 0 1419.936768 Tm 25.297222 0 Td 48.251029 Tz (variables ) Tj 2.741667 0 Td 57.685185 Tz (coded ) Tj 1.827778 0 Td 48.611111 Tz (for ) Tj 0.991667 0 Td 52.662037 Tz (comparison\), ) Tj 3.927778 0 Td 46.118234 Tz (respectively, ) Tj 3.713889 0 Td 48.611111 Tz (as ) Tj 0.738889 0 Td 59.548611 Tz (compared ) Tj 2.955556 0 Td 46.990741 Tz (to ) Tj 51.428571 0 0 51.428571 0 1375.439809 Tm 25.277778 0 Td 50.771605 Tz (the ) Tj 1.088889 0 Td 65.625 Tz (much ) Tj 1.75 0 Td 51.851852 Tz (smaller ) Tj 2.313889 0 Td 46.990741 Tz (linear ) Tj 1.827778 0 Td 53.472222 Tz (time ) Tj 1.438889 0 Td 54.372428 Tz (parameter ) Tj 3.072222 0 Td 49.907407 Tz (estimation ) Tj 3.169444 0 Td 61.574074 Tz (of ) Tj 0.816667 0 Td 55.632716 Tz (-0.072 ) Tj 51.428571 0 0 51.428571 0 1335.831683 Tm 25.316667 0 Td 46.990741 Tz (\(variables ) Tj 3.188889 0 Td 57.037037 Tz (coded ) Tj 2.041667 0 Td 48.611111 Tz (for ) Tj 1.186111 0 Td 52.391975 Tz (comparison\). ) Tj 4.122222 0 Td 48.112536 Tz (Additionally, ) Tj 4.122222 0 Td 51.49177 Tz (canonical ) Tj 51.428571 0 0 51.428571 0 1295.27 Tm 25.297222 0 Td 48.611111 Tz (analysis ) Tj 2.683333 0 Td 55.092593 Tz (of ) Tj 0.875 0 Td 50.771605 Tz (the ) Tj 1.263889 0 Td 58.981481 Tz (model ) Tj 2.119444 0 Td 54.282407 Tz (demonstrated ) Tj 4.238889 0 Td 50.231481 Tz (negative ) Tj 2.702778 0 Td 49.421296 Tz (eigenvalues, ) Tj 51.428571 0 0 51.428571 0 1252.27574 Tm 25.297222 0 Td 53.347578 Tz (corresponding ) Tj 4.297222 0 Td 45.37037 Tz (to ) Tj 0.661111 0 Td 58.333333 Tz (a ) Tj 0.505556 0 Td 68.981481 Tz (maximum ) Tj 3.072222 0 Td 47.638889 Tz (linearized ) Tj 2.975 0 Td 63.734568 Tz (ACt ) Tj 1.263889 0 Td 53.472222 Tz (response ) Tj 2.683333 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 51.851852 Tz (0.62 ) Tj 51.428571 0 0 51.428571 0 1212.563107 Tm 25.297222 0 Td 59.953704 Tz (on ) Tj 0.894444 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 49.537037 Tz (Surface ) Tj 2.275 0 Td 66.975309 Tz (map ) Tj 1.380556 0 Td 43.75 Tz (at ) Tj 0.661111 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 58.333333 Tz (coded ) Tj 1.905556 0 Td 53.914141 Tz (independent ) Tj 3.713889 0 Td 47.314815 Tz (stationary ) Tj 2.994444 0 Td 51.203704 Tz (point ) Tj 51.428571 0 0 51.428571 0 1169.58 Tm 25.297222 0 Td 51.851852 Tz (value ) Tj 1.711111 0 Td 59.953704 Tz (of ) Tj 0.836111 0 Td 52.314815 Tz (-0.096, ) Tj 2.372222 0 Td 53.347578 Tz (corresponding ) Tj 4.355556 0 Td 45.37037 Tz (to ) Tj 0.758333 0 Td 51.851852 Tz (21.7 ) Tj 1.419444 0 Td 54.444444 Tz (hours ) Tj 1.808333 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 51.851852 Tz (exposure. ) Tj 51.428571 0 0 51.428571 0 1125.581395 Tm 25.997222 0 Td 77.777778 Tz (Now ) Tj 1.575 0 Td 49.583333 Tz (refereeing ) Tj 3.111111 0 Td 42.12963 Tz (to ) Tj 0.719444 0 Td 57.037037 Tz (FIGS. ) Tj 1.886111 0 Td 58.333333 Tz (12 ) Tj 0.836111 0 Td 93.981481 Tz (& ) Tj 0.777778 0 Td 46.450617 Tz (13, ) Tj 1.030556 0 Td 61.574074 Tz (show ) Tj 1.672222 0 Td 49.691358 Tz (the ) Tj 1.030556 0 Td 47.638889 Tz (linearized ) Tj 3.013889 0 Td 62.654321 Tz (ACt ) Tj 51.428571 0 0 51.428571 0 1086.548718 Tm 25.297222 0 Td 49.421296 Tz (data ) Tj 1.438889 0 Td 43.75 Tz (in ) Tj 0.738889 0 Td 74.537037 Tz (2D ) Tj 1.069444 0 Td 43.75 Tz (plots. ) Tj 1.866667 0 Td 53.472222 Tz (FIG. ) Tj 1.555556 0 Td 56.712963 Tz (12 ) Tj 0.894444 0 Td 59.62963 Tz (shows ) Tj 2.002778 0 Td 66.975309 Tz (how ) Tj 1.438889 0 Td 55.092593 Tz (coding ) Tj 2.197222 0 Td 48.611111 Tz (the ) Tj 1.088889 0 Td 48.021886 Tz (categorical ) Tj 48.571429 0 0 48.571429 0 1043 Tm 26.764706 0 Td 54.520697 Tz (treatment ) Tj 3.047059 0 Td 51.470588 Tz (variable ) Tj 2.573529 0 Td 57.843137 Tz (allowed ) Tj 2.511765 0 Td 54.901961 Tz (for ) Tj 1.05 0 Td 49.358974 Tz (linearization ) Tj 3.952941 0 Td 51.470588 Tz (in ) Tj 0.7 0 Td 47.181373 Tz (this ) Tj 1.276471 0 Td 56.27451 Tz (dimension; ) Tj 51.428571 0 0 51.428571 0 1001.951456 Tm 25.297222 0 Td 47.962963 Tz (linearized ) Tj 3.052778 0 Td 61.574074 Tz (ACt ) Tj 1.302778 0 Td 49.691358 Tz (values ) Tj 1.963889 0 Td 49.826389 Tz (increase ) Tj 2.527778 0 Td 62.87037 Tz (\(iNOS ) Tj 2.061111 0 Td 59.953704 Tz (up ) Tj 0.894444 0 Td 48.316498 Tz (regulation\) ) Tj 3.325 0 Td 58.333333 Tz (from ) Tj 1.594444 0 Td 55.092593 Tz (-1 ) Tj 51.428571 0 0 51.428571 0 958 Tm 25.316667 0 Td 54.444444 Tz (\(F1+\) ) Tj 1.788889 0 Td 42.12963 Tz (to ) Tj 0.758333 0 Td 32.407407 Tz (1 ) Tj 0.427778 0 Td 52.391975 Tz (\(LPS\). ) Tj 2.022222 0 Td 63.734568 Tz (The ) Tj 1.283333 0 Td 50.231481 Tz (data ) Tj 1.341667 0 Td 49.691358 Tz (are ) Tj 1.030556 0 Td 61.168981 Tz (somewhat ) Tj 3.091667 0 Td 59.41358 Tz (skewed ) Tj 2.255556 0 Td 46.990741 Tz (to ) Tj 0.738889 0 Td 47.530864 Tz (the ) Tj 1.011111 0 Td 39.699074 Tz (left ) Tj 1.088889 0 Td 58.333333 Tz (of ) Tj 51.428571 0 0 51.428571 0 917.877637 Tm 25.277778 0 Td 50.771605 Tz (the ) Tj 1.302778 0 Td 58.333333 Tz (24 ) Tj 1.05 0 Td 56.712963 Tz (hour ) Tj 1.730556 0 Td 54.282407 Tz (time ) Tj 1.652778 0 Td 50.555556 Tz (point ) Tj 1.886111 0 Td 58.333333 Tz (which ) Tj 2.119444 0 Td 62.654321 Tz (was ) Tj 1.477778 0 Td 49.421296 Tz (also ) Tj 1.594444 0 Td 46.090535 Tz (reflected ) Tj 2.838889 0 Td 46.990741 Tz (in ) Tj 0.933333 0 Td 50.771605 Tz (the ) Tj 51.428571 0 0 51.428571 0 876.326582 Tm 25.297222 0 Td 53.06713 Tz (response ) Tj 2.897222 0 Td 49.537037 Tz (Surface ) Tj 2.45 0 Td 59.62963 Tz (model ) Tj 2.119444 0 Td 58.981481 Tz (which ) Tj 2.119444 0 Td 54.282407 Tz (demonstrated ) Tj 4.238889 0 Td 58.333333 Tz (a ) Tj 0.719444 0 Td 69.444444 Tz (maximum ) Tj 51.428571 0 0 51.428571 0 835.142857 Tm 25.297222 0 Td 51.851852 Tz (value ) Tj 1.711111 0 Td 46.990741 Tz (at ) Tj 0.738889 0 Td 53.472222 Tz (21.7 ) Tj 1.438889 0 Td 53.796296 Tz (hours ) Tj 1.808333 0 Td 59.953704 Tz (of ) Tj 0.797222 0 Td 54.6875 Tz (exposure ) Tj 2.8 0 Td 47.962963 Tz (time. ) Tj 51.428571 0 0 51.428571 0 795.195954 Tm 26.036111 0 Td 55.092593 Tz (FIG. ) Tj 1.536111 0 Td 55.092593 Tz (13 ) Tj 0.894444 0 Td 52.662037 Tz (demonstrates ) Tj 3.986111 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 50.05144 Tz (parabolic ) Tj 2.877778 0 Td 57.361111 Tz (dependency ) Tj 3.636111 0 Td 52.662037 Tz (with ) Tj 1.458333 0 Td 54.282407 Tz (time ) Tj 51.428571 0 0 51.428571 0 752.184539 Tm 25.297222 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 48.611111 Tz (the ) Tj 1.069444 0 Td 47.962963 Tz (linearized ) Tj 3.072222 0 Td 61.574074 Tz (ACt ) Tj 1.302778 0 Td 49.421296 Tz (data ) Tj 1.341667 0 Td 51.851852 Tz (as ) Tj 0.816667 0 Td 50.771605 Tz (values ) Tj 2.002778 0 Td 52.932099 Tz (obviously ) Tj 3.013889 0 Td 58.333333 Tz (peak ) Tj 1.555556 0 Td 46.990741 Tz (at ) Tj 0.738889 0 Td 48.611111 Tz (the ) Tj 1.05 0 Td 58.333333 Tz (24 ) Tj 51.428571 0 0 51.428571 0 711.81761 Tm 25.277778 0 Td 49.691358 Tz (hours. ) Tj 2.002778 0 Td 54.012346 Tz (Although, ) Tj 3.130556 0 Td 55.092593 Tz (time ) Tj 1.555556 0 Td 37.268519 Tz (is ) Tj 0.680556 0 Td 49.421296 Tz (also ) Tj 1.419444 0 Td 57.037037 Tz (coded ) Tj 1.944444 0 Td 45.37037 Tz (in ) Tj 0.758333 0 Td 50.771605 Tz (the ) Tj 1.147222 0 Td 52.662037 Tz (response ) Tj 2.741667 0 Td 50 Tz (Surface ) Tj 51.428571 0 0 51.428571 0 672.278351 Tm 25.297222 0 Td 54.012346 Tz (procedure ) Tj 3.266667 0 Td 51.851852 Tz (so ) Tj 0.972222 0 Td 46.180556 Tz (that ) Tj 1.419444 0 Td 54.73251 Tz (parameter ) Tj 3.266667 0 Td 50.05144 Tz (estimates ) Tj 3.072222 0 Td 66.975309 Tz (may ) Tj 1.555556 0 Td 58.333333 Tz (be ) Tj 1.05 0 Td 59.953704 Tz (compared ) Tj 51.428571 0 0 51.428571 0 629.119497 Tm 25.297222 0 Td 66.759259 Tz (among ) Tj 2.275 0 Td 45.730453 Tz (different ) Tj 2.761111 0 Td 47.530864 Tz (variables ) Tj 2.858333 0 Td 45.775463 Tz (directly ) Tj 2.488889 0 Td 45.37037 Tz (in ) Tj 0.836111 0 Td 50.771605 Tz (taking ) Tj 2.138889 0 Td 47.800926 Tz (variable ) Tj 2.566667 0 Td 51.851852 Tz (value ) Tj 51.428571 0 0 51.428571 0 585.617329 Tm 25.297222 0 Td 52.391975 Tz (ranges ) Tj 2.022222 0 Td 46.990741 Tz (into ) Tj 1.263889 0 Td 48.611111 Tz (consideration. ) Tj 4.2 0 Td 53.472222 Tz (This ) Tj 1.458333 0 Td 43.75 Tz (rise ) Tj 1.147222 0 Td 59.41358 Tz (and ) Tj 1.225 0 Td 38.888889 Tz (fall ) Tj 1.108333 0 Td 49.691358 Tz (nature ) Tj 1.905556 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 49.691358 Tz (the ) Tj 1.030556 0 Td 57.523148 Tz (gene ) Tj 51.428571 0 0 51.428571 0 546.897959 Tm 25.297222 0 Td 51.527778 Tz (expression ) Tj 3.286111 0 Td 66.975309 Tz (may ) Tj 1.341667 0 Td 59.953704 Tz (be ) Tj 0.875 0 Td 47.685185 Tz (related ) Tj 2.1 0 Td 48.611111 Tz (to ) Tj 0.719444 0 Td 47.530864 Tz (either ) Tj 1.808333 0 Td 58.333333 Tz (an ) Tj 0.836111 0 Td 52.146465 Tz (endocytosis ) Tj 3.597222 0 Td 51.851852 Tz (process ) Tj 2.352778 0 Td 46.990741 Tz (in ) Tj 51.428571 0 0 51.428571 0 506.969732 Tm 25.277778 0 Td 59.62963 Tz (which ) Tj 1.925 0 Td 54.012346 Tz (inflammatory ) Tj 4.005556 0 Td 51.851852 Tz (agents ) Tj 2.002778 0 Td 49.691358 Tz (are ) Tj 0.972222 0 Td 55.740741 Tz (being ) Tj 1.75 0 Td 48.971193 Tz (depleted, ) Tj 2.780556 0 Td 49.826389 Tz (receptor ) Tj 2.488889 0 Td 51.851852 Tz (inter ) Tj 51.428571 0 0 51.428571 0 462.617225 Tm 25.297222 0 Td 47.638889 Tz (nalization ) Tj 3.111111 0 Td 59.41358 Tz (and ) Tj 1.322222 0 Td 51.649306 Tz (down-regulation, ) Tj 5.211111 0 Td 53.472222 Tz (or ) Tj 0.875 0 Td 49.826389 Tz (receptor ) Tj 2.644444 0 Td 48.179012 Tz (desensitization ) Tj 51.428571 0 0 51.428571 0 420.26087 Tm 25.297222 0 Td 58.333333 Tz (from ) Tj 1.788889 0 Td 47.962963 Tz (over-activation ) Tj 4.725 0 Td 58.333333 Tz (of ) Tj 0.972222 0 Td 50.771605 Tz (the ) Tj 1.302778 0 Td 47.530864 Tz (signal ) Tj 2.1 0 Td 49.691358 Tz (transduction ) Tj 3.966667 0 Td 53.877315 Tz (pathway. ) Tj 51.428571 0 0 51.428571 0 379.401198 Tm 25.297222 0 Td 58.333333 Tz (These ) Tj 1.886111 0 Td 54.012346 Tz (graphs ) Tj 2.1 0 Td 47.222222 Tz (clearly ) Tj 2.158333 0 Td 61.574074 Tz (show ) Tj 1.691667 0 Td 48.611111 Tz (the ) Tj 1.05 0 Td 55.092593 Tz (dependent ) Tj 3.130556 0 Td 46.367521 Tz (linearization ) Tj 3.772222 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 50.771605 Tz (the ) Tj 51.428571 0 0 51.428571 0 338.451348 Tm 25.297222 0 Td 51.041667 Tz (data ) Tj 1.302778 0 Td 50 Tz (against ) Tj 2.236111 0 Td 46.8107 Tz (material, ) Tj 2.625 0 Td 50.771605 Tz (the ) Tj 1.05 0 Td 53.619529 Tz (independent ) Tj 3.675 0 Td 46.342593 Tz (covariate, ) Tj 2.897222 0 Td 59.41358 Tz (and ) Tj 1.166667 0 Td 50.771605 Tz (the ) Tj 0.991667 0 Td 63.194444 Tz (para ) Tj 51.428571 0 0 51.428571 0 295.368159 Tm 25.277778 0 Td 48.611111 Tz (bolic ) Tj 1.672222 0 Td 53.06713 Tz (response ) Tj 2.702778 0 Td 59.953704 Tz (of ) Tj 0.797222 0 Td 49.691358 Tz (the ) Tj 1.088889 0 Td 49.421296 Tz (data ) Tj 1.4 0 Td 43.75 Tz (in ) Tj 0.758333 0 Td 49.537037 Tz (regards ) Tj 2.275 0 Td 45.37037 Tz (to ) Tj 0.758333 0 Td 47.962963 Tz (time. ) Tj 1.613889 0 Td 57.685185 Tz (These ) Tj 1.905556 0 Td 59.722222 Tz (observa ) Tj 51.428571 0 0 51.428571 0 252.230047 Tm 25.277778 0 Td 47.314815 Tz (tions ) Tj 1.594444 0 Td 46.990741 Tz (validate ) Tj 2.372222 0 Td 48.611111 Tz (the ) Tj 0.991667 0 Td 47.530864 Tz (selection ) Tj 2.722222 0 Td 51.851852 Tz (of ) Tj 0.680556 0 Td 58.333333 Tz (a ) Tj 0.447222 0 Td 53.20216 Tz (second-order ) Tj 3.927778 0 Td 53.06713 Tz (response ) Tj 2.663889 0 Td 50 Tz (Surface ) Tj 51.428571 0 0 51.428571 0 212.022642 Tm 25.297222 0 Td 59.62963 Tz (model ) Tj 2.080556 0 Td 51.851852 Tz (with ) Tj 1.497222 0 Td 50.106838 Tz (incorporation ) Tj 4.141667 0 Td 59.953704 Tz (of ) Tj 0.855556 0 Td 51.851852 Tz (a ) Tj 0.563889 0 Td 47.530864 Tz (linear ) Tj 1.866667 0 Td 46.990741 Tz (covariate, ) Tj 3.052778 0 Td 57.685185 Tz (hence ) Tj 1.963889 0 Td 51.851852 Tz (a ) Tj 0.525 0 Td 76.157407 Tz (3D ) Tj 40 0 0 40 0 176 Tm 32.525 0 Td 70.138889 Tz (model. ) Tj ET Q endstream endobj 476 0 obj << /Length 1 >> stream q endstream endobj 477 0 obj << /Length 22756 >> stream Q q 0.24 0 0 0.24 0 0 cm BT /F1 1 Tf 3 Tr 62.857143 0 0 62.857143 0 3023 Tm 17.325 0 Td 83.522727 Tz (US ) Tj 1.240909 0 Td 58.333333 Tz (8,404,256 ) Tj 3.388636 0 Td 74.242424 Tz (B2 ) Tj 52.857143 0 0 52.857143 0 2968 Tm 14.208108 0 Td 78.828829 Tz (27 ) Tj 34.285714 0 0 34.285714 0 2913 Tm 24.733333 0 Td 107.916667 Tz (TABLE ) Tj 3.966667 0 Td 87.5 Tz (4 ) Tj 38.571429 0 0 38.571429 0 2841.688372 Tm 17.785185 0 Td 74.176955 Tz (Primer ) Tj 3.137037 0 Td 74.320988 Tz (Pairs ) Tj 2.696296 0 Td 75.617284 Tz (Used ) Tj 2.333333 0 Td 72.016461 Tz (for ) Tj 1.814815 0 Td 74.691358 Tz (qRT-PCR ) Tj 52.857143 0 0 52.857143 0 2968 Tm 32.635135 0 Td 77.252252 Tz (28 ) Tj 40 0 0 40 0 2806 Tm 17.175 0 Td 70.833333 Tz (Primer ) Tj 3.05 0 Td 70 Tz (Pairs ) Tj 2.575 0 Td 72.916667 Tz (Used ) Tj 2.25 0 Td 68.055556 Tz (for ) Tj 1.7 0 Td 72.619048 Tz (CRT-PCR ) Tj 38.571429 0 0 38.571429 0 2747 Tm 35.311111 0 Td 75.185185 Tz (efficiency ) Tj 35.428571 0 0 35.428571 0 2709.462838 Tm 10.302419 0 Td 81.149194 Tz (Gene ) Tj 3.274194 0 Td 82.063919 Tz (direction ) Tj 4.71371 0 Td 81.384409 Tz (nucleotide ) Tj 5.419355 0 Td 81.018519 Tz (sequences ) Tj 16.625 0 Td 59.587814 Tz (\(E\) ) Tj 31.190476 0 0 31.190476 0 2649.153741 Tm 11.830534 0 Td 85.496183 Tz (iNOS ) Tj 4.777099 0 Td 113.549618 Tz (SeSe ) Tj 4.2 0 Td 92.620865 Tz (5'-TAG ) Tj 3.879389 0 Td 90.839695 Tz (GTC ) Tj 2.212214 0 Td 92.620865 Tz (GAT ) Tj 2.276336 0 Td 94.402036 Tz (GCA ) Tj 2.244275 0 Td 94.402036 Tz (CAA ) Tj 2.244275 0 Td 92.620865 Tz (CTG ) Tj 2.244275 0 Td 90.839695 Tz (GGT ) Tj 2.212214 0 Td 89.770992 Tz (GAA-3 ) Tj 3.01374 0 Td 61.450382 Tz ('; ) Tj 4.296183 0 Td 112.21374 Tz (171 ) Tj 32.857143 0 0 32.857143 0 2615 Tm 19.721739 0 Td 86.231884 Tz (SEO ) Tj 2.16087 0 Td 83.695652 Tz (ID ) Tj 1.521739 0 Td 79.468599 Tz (NO. ) Tj 2.221739 0 Td 60.869565 Tz (1 ) Tj 32.539683 0 0 32.539683 0 2581.549669 Tm 15.888293 0 Td 85.02439 Tz (anti- ) Tj 4.056585 0 Td 87.926829 Tz (5'-TCT ) Tj 3.687805 0 Td 90.487805 Tz (TTG ) Tj 2.120488 0 Td 92.195122 Tz (ACG ) Tj 2.212683 0 Td 87.073171 Tz (CTC ) Tj 2.15122 0 Td 90.487805 Tz (GGA ) Tj 2.089756 0 Td 90.487805 Tz (ACT ) Tj 2.181951 0 Td 92.195122 Tz (GTA ) Tj 2.15122 0 Td 85.365854 Tz (GCA-3' ) Tj 30.571429 0 0 30.571429 0 2549.471861 Tm 16.943925 0 Td 115.84891 Tz (SeSe ) Tj 4.252336 0 Td 92.679128 Tz (SEO ) Tj 2.32243 0 Td 89.953271 Tz (ID ) Tj 1.635514 0 Td 85.410177 Tz (NO. ) Tj 2.28972 0 Td 81.775701 Tz (2 ) Tj 30.779221 0 0 30.779221 0 2483.253652 Tm 11.566245 0 Td 116.420534 Tz (R18s ) Tj 5.263291 0 Td 115.066807 Tz (SeSe ) Tj 4.256118 0 Td 92.955931 Tz (5'-TAT ) Tj 3.898734 0 Td 93.858415 Tz (TGC ) Tj 2.274262 0 Td 97.468354 Tz (TCA ) Tj 2.274262 0 Td 93.858415 Tz (ATC ) Tj 2.306751 0 Td 93.858415 Tz (TCG ) Tj 2.241772 0 Td 93.858415 Tz (GGT ) Tj 2.274262 0 Td 92.053446 Tz (GC ) Tj 1.689451 0 Td 91.279887 Tz (TGA-3"; ) Tj 7.992405 0 Td 87.992264 Tz (1.65 ) Tj 32.857143 0 0 32.857143 0 2448 Tm 19.721739 0 Td 86.231884 Tz (SEO ) Tj 2.16087 0 Td 83.695652 Tz (ID ) Tj 1.521739 0 Td 79.468599 Tz (NO. ) Tj 2.16087 0 Td 71.014493 Tz (3 ) Tj 27.402597 0 0 27.402597 0 2419 Tm 18.866825 0 Td 100.963665 Tz (anti- ) Tj 4.817062 0 Td 85.150079 Tz (5 ) Tj 1.313744 0 Td 66.903633 Tz (- ) Tj 0.583886 0 Td 103.396524 Tz (CCA ) Tj 2.481517 0 Td 107.45129 Tz (GAG ) Tj 2.627488 0 Td 79.06793 Tz (CGA. ) Tj 2.445024 0 Td 109.478673 Tz (AAG ) Tj 2.627488 0 Td 103.396524 Tz (CAT ) Tj 2.518009 0 Td 107.45129 Tz (TTG ) Tj 2.590995 0 Td 103.396524 Tz (CCA ) Tj 2.445024 0 Td 102.382833 Tz (AGA-3' ) Tj 30.571429 0 0 30.571429 0 2383.478261 Tm 16.943925 0 Td 115.84891 Tz (SeSe ) Tj 4.252336 0 Td 92.679128 Tz (SEO ) Tj 2.32243 0 Td 89.953271 Tz (ID ) Tj 1.635514 0 Td 85.410177 Tz (NO. ) Tj 2.32243 0 Td 76.323988 Tz (4 ) Tj 30.952381 0 0 30.952381 0 2316.403061 Tm 10.661538 0 Td 83.461538 Tz (B- ) Tj 1.227692 0 Td 91.538462 Tz (-Actin ) Tj 4.846154 0 Td 114.423077 Tz (SeSe ) Tj 4.232308 0 Td 92.435897 Tz (5'-ACT ) Tj 3.909231 0 Td 91.538462 Tz (CCT ) Tj 2.261538 0 Td 91.538462 Tz (GCT ) Tj 2.261538 0 Td 91.538462 Tz (TGC ) Tj 2.261538 0 Td 95.128205 Tz (TGA ) Tj 2.261538 0 Td 91.538462 Tz (TCC ) Tj 2.229231 0 Td 96.923077 Tz (ACA ) Tj 2.229231 0 Td 90.461538 Tz (TCT-3 ) Tj 3.036923 0 Td 16.153846 Tz ('' ) Tj 0.581538 0 Td 26.923077 Tz (. ) Tj 3.715385 0 Td 75.384615 Tz (2. ) Tj 1.195385 0 Td 78.076923 Tz (64 ) Tj 32.857143 0 0 32.857143 0 2282 Tm 19.721739 0 Td 86.231884 Tz (SEO ) Tj 2.191304 0 Td 81.15942 Tz (ID ) Tj 1.491304 0 Td 86.231884 Tz (NO ) Tj 1.704348 0 Td 65.942029 Tz (5 ) Tj 32.571429 0 0 32.571429 0 2249.549669 Tm 15.872807 0 Td 84.94152 Tz (anti- ) Tj 4.052632 0 Td 88.693957 Tz (5'-TCG ) Tj 3.714912 0 Td 86.988304 Tz (TAC ) Tj 2.149123 0 Td 86.988304 Tz (CAC ) Tj 2.087719 0 Td 92.105263 Tz (AGG ) Tj 2.210526 0 Td 86.988304 Tz (CAT ) Tj 2.118421 0 Td 88.693957 Tz (TGT ) Tj 2.179825 0 Td 86.988304 Tz (GAT ) Tj 2.118421 0 Td 85.964912 Tz (GGA-3 ) Tj 2.885965 0 Td 58.845029 Tz ('; ) Tj 30.571429 0 0 30.571429 0 2217.491228 Tm 16.943925 0 Td 115.84891 Tz (SeSe ) Tj 4.252336 0 Td 92.679128 Tz (SEO ) Tj 2.32243 0 Td 89.953271 Tz (ID ) Tj 1.635514 0 Td 85.410177 Tz (NO. ) Tj 2.38785 0 Td 65.420561 Tz (6 ) Tj 22.857143 0 0 22.857143 0 2156 Tm 14.35 0 Td 100.625 Tz (Note: ) Tj 32.857143 0 0 32.857143 0 2121.769133 Tm 9.982609 0 Td 75.241546 Tz (Primer ) Tj 3.195652 0 Td 75.072464 Tz (pairs ) Tj 2.769565 0 Td 74.818841 Tz (were ) Tj 2.343478 0 Td 74.818841 Tz (designed ) Tj 4.13913 0 Td 74.057971 Tz (using ) Tj 2.8 0 Td 74.057971 Tz (Integrated ) Tj 4.96087 0 Td 76.086957 Tz (DNA ) Tj 1.856522 0 Td 76.086957 Tz (Technologies ) Tj 32.857143 0 0 32.857143 0 2072.410569 Tm 9.982609 0 Td 73.550725 Tz (to ) Tj 1.33913 0 Td 76.086957 Tz (poor ) Tj 2.343478 0 Td 72.397892 Tz (efficiency. ) Tj 51.428571 0 0 51.428571 0 1968.781638 Tm 7.097222 0 Td 75.617284 Tz (Now ) Tj 1.516667 0 Td 47.170782 Tz (referring ) Tj 2.663889 0 Td 43.75 Tz (to ) Tj 0.680556 0 Td 57.037037 Tz (FIGS. ) Tj 1.866667 0 Td 59.548611 Tz (11A-11C, ) Tj 2.994444 0 Td 46.8107 Tz (injection ) Tj 2.663889 0 Td 48.611111 Tz (delivery ) Tj 2.45 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 62.654321 Tz (F1+ ) Tj 51.428571 0 0 51.428571 0 1923.252362 Tm 6.358333 0 Td 46.990741 Tz (into ) Tj 1.458333 0 Td 42.12963 Tz (rat ) Tj 1.011111 0 Td 58.333333 Tz (Ca-2 ) Tj 1.730556 0 Td 58.333333 Tz (and ) Tj 1.361111 0 Td 46.990741 Tz (-3 ) Tj 0.913889 0 Td 49.691358 Tz (vertebrae ) Tj 2.955556 0 Td 53.703704 Tz (through ) Tj 2.547222 0 Td 55.092593 Tz (a ) Tj 0.622222 0 Td 52.391975 Tz (burred ) Tj 2.197222 0 Td 52.662037 Tz (hole ) Tj 1.575 0 Td 60.493827 Tz (was ) Tj 51.428571 0 0 51.428571 0 1883.313939 Tm 6.377778 0 Td 48.881173 Tz (Successfully ) Tj 3.791667 0 Td 53.596866 Tz (accomplished, ) Tj 4.433333 0 Td 58.333333 Tz (and ) Tj 1.302778 0 Td 49.691358 Tz (the ) Tj 1.263889 0 Td 47.800926 Tz (B-tricalcium ) Tj 3.713889 0 Td 54.73251 Tz (phosphate ) Tj 51.428571 0 0 51.428571 0 1838.740385 Tm 6.358333 0 Td 60.133745 Tz (component ) Tj 3.441667 0 Td 60.493827 Tz (was ) Tj 1.302778 0 Td 46.296296 Tz (clearly ) Tj 2.158333 0 Td 48.82716 Tz (distinguishable ) Tj 4.569444 0 Td 57.523148 Tz (from ) Tj 1.555556 0 Td 51.851852 Tz (the ) Tj 1.108333 0 Td 49.151235 Tz (native ) Tj 1.925 0 Td 59.143519 Tz (bone ) Tj 51.428571 0 0 51.428571 0 1795 Tm 6.358333 0 Td 59.41358 Tz (due ) Tj 1.186111 0 Td 45.37037 Tz (to ) Tj 0.680556 0 Td 58.333333 Tz (a ) Tj 0.447222 0 Td 52.932099 Tz (higher ) Tj 2.041667 0 Td 46.720679 Tz (radiopacity. ) Tj 3.538889 0 Td 54.282407 Tz (FIG. ) Tj 1.497222 0 Td 65.895062 Tz (11C ) Tj 1.302778 0 Td 59.62963 Tz (shows ) Tj 1.944444 0 Td 49.691358 Tz (the ) Tj 1.011111 0 Td 74.537037 Tz (3D ) Tj 1.05 0 Td 51.131687 Tz (rendering ) Tj 51.428571 0 0 51.428571 0 1748.165049 Tm 6.358333 0 Td 58.333333 Tz (of ) Tj 0.816667 0 Td 49.331276 Tz (delivered ) Tj 2.877778 0 Td 48.611111 Tz (material ) Tj 2.566667 0 Td 43.75 Tz (in ) Tj 0.738889 0 Td 40.185185 Tz (situ; ) Tj 1.419444 0 Td 56.532922 Tz (automated ) Tj 3.227778 0 Td 52.256944 Tz (software ) Tj 2.683333 0 Td 48.611111 Tz (analysis ) Tj 2.527778 0 Td 58.333333 Tz (of ) Tj 51.428571 0 0 51.428571 0 1705.104218 Tm 6.338889 0 Td 44.560185 Tz (this ) Tj 1.302778 0 Td 52.5 Tz (bolus ) Tj 1.827778 0 Td 49.259259 Tz (calculated ) Tj 3.188889 0 Td 55.092593 Tz (a ) Tj 0.680556 0 Td 48.070988 Tz (B-tricalcium ) Tj 3.713889 0 Td 55.092593 Tz (phosphate ) Tj 3.227778 0 Td 59.953704 Tz (Volume ) Tj 2.391667 0 Td 63.734568 Tz (per ) Tj 51.428571 0 0 51.428571 0 1662.72549 Tm 6.358333 0 Td 53.240741 Tz (centage ) Tj 2.411111 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 57.253086 Tz (22.8%, ) Tj 2.236111 0 Td 53.472222 Tz (only ) Tj 1.477778 0 Td 62.384259 Tz (2.8% ) Tj 1.691667 0 Td 52.391975 Tz (higher ) Tj 2.061111 0 Td 51.041667 Tz (than ) Tj 1.438889 0 Td 48.611111 Tz (the ) Tj 1.069444 0 Td 45.959596 Tz (theoretical ) Tj 3.227778 0 Td 59.41358 Tz (for ) Tj 52.857143 0 0 52.857143 0 1617.513447 Tm 6.186486 0 Td 52.027027 Tz (mulation ) Tj 2.705405 0 Td 47.034535 Tz (calculations ) Tj 3.613514 0 Td 49.399399 Tz (\(Table ) Tj 1.967568 0 Td 40.990991 Tz (4\). ) Tj 0.927027 0 Td 85.135135 Tz (A ) Tj 0.7 0 Td 55.705706 Tz (low ) Tj 1.248649 0 Td 52.027027 Tz (degree ) Tj 2.043243 0 Td 58.333333 Tz (of ) Tj 0.813514 0 Td 49.81982 Tz (anisotropy ) Tj 51.428571 0 0 51.428571 0 1571 Tm 6.377778 0 Td 61.574074 Tz (\(DA\) ) Tj 1.652778 0 Td 50.555556 Tz (value ) Tj 1.613889 0 Td 61.574074 Tz (of ) Tj 0.816667 0 Td 49.421296 Tz (1.04 ) Tj 1.341667 0 Td 61.574074 Tz (was ) Tj 1.244444 0 Td 47.800926 Tz (also ) Tj 1.302778 0 Td 54.444444 Tz (determined ) Tj 3.383333 0 Td 53.472222 Tz (with ) Tj 1.4 0 Td 50.771605 Tz (the ) Tj 1.05 0 Td 51.446759 Tz (software ) Tj 2.586111 0 Td 60.493827 Tz (and ) Tj 51.428571 0 0 51.428571 0 1530.998716 Tm 6.358333 0 Td 38.888889 Tz (is ) Tj 0.758333 0 Td 45.37037 Tz (in ) Tj 0.797222 0 Td 56.893004 Tz (agreement ) Tj 3.305556 0 Td 58.333333 Tz (of ) Tj 0.855556 0 Td 50.771605 Tz (the ) Tj 1.186111 0 Td 48.251029 Tz (spherical ) Tj 2.916667 0 Td 49.691358 Tz (nature ) Tj 2.061111 0 Td 56.712963 Tz (of ) Tj 0.836111 0 Td 50.771605 Tz (the ) Tj 1.302778 0 Td 48.070988 Tz (B-tricalcium ) Tj 51.428571 0 0 51.428571 0 1490.848635 Tm 6.358333 0 Td 54.73251 Tz (phosphate ) Tj 3.188889 0 Td 50.636574 Tz (granules ) Tj 2.722222 0 Td 64.166667 Tz (\(DA-1 ) Tj 2.236111 0 Td 54.444444 Tz (being ) Tj 1.847222 0 Td 55.092593 Tz (a ) Tj 0.563889 0 Td 48.148148 Tz (perfect ) Tj 2.255556 0 Td 47.800926 Tz (sphere\). ) Tj 2.566667 0 Td 57.79321 Tz (Images ) Tj 51.428571 0 0 51.428571 0 1441.939976 Tm 6.358333 0 Td 58.333333 Tz (and ) Tj 1.186111 0 Td 48.070988 Tz (calculations ) Tj 3.636111 0 Td 61.033951 Tz (showed ) Tj 2.352778 0 Td 44.560185 Tz (that ) Tj 1.205556 0 Td 48.611111 Tz (the ) Tj 1.030556 0 Td 54.552469 Tz (biocomposite ) Tj 4.063889 0 Td 61.574074 Tz (was ) Tj 1.225 0 Td 51.851852 Tz (able ) Tj 1.380556 0 Td 45.37037 Tz (to ) Tj 0.680556 0 Td 59.953704 Tz (be ) Tj 51.428571 0 0 51.428571 0 1398.142857 Tm 6.358333 0 Td 48.611111 Tz (localized ) Tj 2.761111 0 Td 50.231481 Tz (as ) Tj 0.758333 0 Td 55.092593 Tz (an ) Tj 0.816667 0 Td 48.206019 Tz (injected ) Tj 2.411111 0 Td 53.148148 Tz (bolus ) Tj 1.769444 0 Td 50.771605 Tz (within ) Tj 1.963889 0 Td 51.203704 Tz (cancellous ) Tj 3.208333 0 Td 53.148148 Tz (bone, ) Tj 1.730556 0 Td 58.333333 Tz (a ) Tj 0.486111 0 Td 64.814815 Tz (mini ) Tj 51.428571 0 0 51.428571 0 1359 Tm 6.358333 0 Td 54.444444 Tz (mally ) Tj 1.847222 0 Td 49.016204 Tz (invasive ) Tj 2.527778 0 Td 48.611111 Tz (delivery ) Tj 2.508333 0 Td 51.527778 Tz (procedure. ) Tj 51.428571 0 0 51.428571 0 1315.452736 Tm 7.116667 0 Td 54.552469 Tz (Inflammatory ) Tj 4.083333 0 Td 52.211934 Tz (responses ) Tj 2.975 0 Td 49.826389 Tz (inherent ) Tj 2.508333 0 Td 46.990741 Tz (to ) Tj 0.719444 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 53.472222 Tz (base ) Tj 1.458333 0 Td 49.016204 Tz (material ) Tj 2.527778 0 Td 58.333333 Tz (were ) Tj 51.428571 0 0 51.428571 0 1273.751465 Tm 6.358333 0 Td 57.685185 Tz (found ) Tj 1.788889 0 Td 59.41358 Tz (and ) Tj 1.205556 0 Td 53.148148 Tz (could ) Tj 1.691667 0 Td 59.953704 Tz (be ) Tj 0.855556 0 Td 56.17284 Tz (modulated ) Tj 3.15 0 Td 53.240741 Tz (through ) Tj 2.391667 0 Td 50.106838 Tz (incorporation ) Tj 4.005556 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 54.444444 Tz (plate ) Tj 32.857143 0 0 32.857143 0 1142 Tm 27.604348 0 Td 87.5 Tz (SEQUENCE ) Tj 4.778261 0 Td 88.405797 Tz (LISTING ) Tj 32.857143 0 0 32.857143 0 1042 Tm 11.13913 0 Td 81.15942 Tz (<16 ) Tj 1.613043 0 Td 71.014493 Tz (Os ) Tj 1.552174 0 Td 88.768116 Tz (NUMBER ) Tj 3.713043 0 Td 86.231884 Tz (OF ) Tj 1.582609 0 Td 87.922705 Tz (SEO ) Tj 2.221739 0 Td 78.623188 Tz (ID ) Tj 1.491304 0 Td 81.15942 Tz (NOS: ) Tj 2.708696 0 Td 65.942029 Tz (6 ) Tj 27.142857 0 0 27.142857 0 980 Tm 13.484211 0 Td 92.105263 Tz (<21 ) Tj 1.989474 0 Td 79.824561 Tz (Oc ) Tj 27.142857 0 0 27.142857 0 946 Tm 13.484211 0 Td 128.947368 Tz (<211 ) Tj 27.142857 0 0 27.142857 0 913 Tm 13.484211 0 Td 104.385965 Tz (<212> ) Tj 27.142857 0 0 27.142857 0 880 Tm 13.484211 0 Td 104.385965 Tz (<213> ) Tj 32.857143 0 0 32.857143 0 976 Tm 14.304348 0 Td 86.231884 Tz (SEO ) Tj 2.16087 0 Td 83.695652 Tz (ID ) Tj 1.521739 0 Td 88.768116 Tz (NO ) Tj 1.704348 0 Td 60.869565 Tz (1 ) Tj 27.142857 0 0 27.142857 0 946 Tm 17.315789 0 Td 101.754386 Tz (LENGTH: ) Tj 5.157895 0 Td 95.175439 Tz (24 ) Tj 27.142857 0 0 27.142857 0 913 Tm 17.315789 0 Td 99.473684 Tz (TYPE: ) Tj 3.868421 0 Td 108.479532 Tz (DNA ) Tj 30 0 0 30 0 880 Tm 15.633333 0 Td 93.82716 Tz (ORGANISM: ) Tj 5.833333 0 Td 96.296296 Tz (Murine ) Tj 32.857143 0 0 32.857143 0 809 Tm 11.13913 0 Td 78.623188 Tz (<4 ) Tj 1.065217 0 Td 76.086957 Tz (OOs ) Tj 2.069565 0 Td 85.668277 Tz (SEQUENCE: ) Tj 5.386957 0 Td 65.942029 Tz (1 ) Tj 34.285714 0 0 34.285714 0 745.160584 Tm 10.675 0 Td 68.055556 Tz (taggit ) Tj 2.566667 0 Td 103.298611 Tz (catg ) Tj 3.033333 0 Td 84.097222 Tz (cacaactggg ) Tj 5.570833 0 Td 111.805556 Tz (taa ) Tj 32.857143 0 0 32.857143 0 639 Tm 14.304348 0 Td 86.231884 Tz (SEO ) Tj 2.16087 0 Td 83.695652 Tz (ID ) Tj 1.521739 0 Td 88.768116 Tz (NO ) Tj 1.613043 0 Td 76.086957 Tz (2 ) Tj 27.142857 0 0 27.142857 0 608 Tm 17.315789 0 Td 101.754386 Tz (LENGTH: ) Tj 5.157895 0 Td 95.175439 Tz (24 ) Tj 27.142857 0 0 27.142857 0 572 Tm 17.315789 0 Td 99.473684 Tz (TYPE: ) Tj 3.868421 0 Td 108.479532 Tz (DNA ) Tj 30 0 0 30 0 537 Tm 15.633333 0 Td 93.82716 Tz (ORGANISM: ) Tj 5.833333 0 Td 96.296296 Tz (Murine ) Tj 32.857143 0 0 32.857143 0 462 Tm 11.13913 0 Td 78.623188 Tz (<4 ) Tj 1.065217 0 Td 76.086957 Tz (OOs ) Tj 2.069565 0 Td 85.668277 Tz (SEQUENCE: ) Tj 5.356522 0 Td 71.014493 Tz (2 ) Tj 34.285714 0 0 34.285714 0 395 Tm 10.675 0 Td 83.611111 Tz (totttgacgc ) Tj 5.570833 0 Td 76.45202 Tz (ticggaactgt ) Tj 5.629167 0 Td 82.638889 Tz (agca ) Tj 32.857143 0 0 32.857143 0 286 Tm 11.13913 0 Td 81.15942 Tz (<21 ) Tj 1.613043 0 Td 68.478261 Tz (Os ) Tj 1.521739 0 Td 89.613527 Tz (SEQ ) Tj 2.16087 0 Td 86.231884 Tz (ID ) Tj 1.582609 0 Td 86.231884 Tz (NO ) Tj 1.613043 0 Td 71.014493 Tz (3 ) Tj 27.142857 0 0 27.142857 0 254 Tm 13.521053 0 Td 101.929825 Tz (&211s ) Tj 3.831579 0 Td 100.877193 Tz (LENGTH: ) Tj 5.121053 0 Td 95.175439 Tz (23 ) Tj 27.142857 0 0 27.142857 0 219 Tm 13.521053 0 Td 82.894737 Tz (&212s. ) Tj 3.757895 0 Td 100.701754 Tz (TYPE: ) Tj 3.868421 0 Td 106.432749 Tz (DNA ) Tj 30 0 0 30 0 2120 Tm 42.133333 0 Td 83.333333 Tz (on-line ) Tj 4.1 0 Td 82.828283 Tz (PRIMERQUEST ) Tj 25.714286 0 0 25.714286 0 2098 Tm 12.794444 0 Td 94.791667 Tz (software ) Tj 5.016667 0 Td 92.901235 Tz (and ) Tj 2.216667 0 Td 92.037037 Tz (NIH's ) Tj 3.266667 0 Td 92.361111 Tz (GENEANK. ) Tj 5.055556 0 Td 95.601852 Tz (Data ) Tj 2.722222 0 Td 95.925926 Tz (associated ) Tj 6.183333 0 Td 95.601852 Tz (with ) Tj 2.8 0 Td 84.259259 Tz (B- ) Tj 1.205556 0 Td 93.981481 Tz (-Actin ) Tj 3.85 0 Td 95.601852 Tz (measures ) Tj 5.055556 0 Td 95.601852 Tz (were ) Tj 2.722222 0 Td 96.502058 Tz (discarded ) Tj 5.716667 0 Td 92.901235 Tz (due ) Tj 30 0 0 30 0 1923 Tm 41.6 0 Td 77.777778 Tz (25 ) Tj 30 0 0 30 0 1716 Tm 41.6 0 Td 80.555556 Tz (30 ) Tj 30 0 0 30 0 1507 Tm 41.6 0 Td 77.777778 Tz (35 ) Tj 51.428571 0 0 51.428571 0 1968.505051 Tm 25.297222 0 Td 41.049383 Tz (let ) Tj 1.011111 0 Td 44.398148 Tz (releasate. ) Tj 2.936111 0 Td 50.968013 Tz (Differences ) Tj 3.616667 0 Td 45.37037 Tz (in ) Tj 0.777778 0 Td 53.877315 Tz (hydrogel ) Tj 2.838889 0 Td 55.632716 Tz (weight ) Tj 2.216667 0 Td 51.388889 Tz (percent ) Tj 2.391667 0 Td 48.611111 Tz (\(2 ) Tj 0.894444 0 Td 42.12963 Tz (vs. ) Tj 51.428571 0 0 51.428571 0 1929.700613 Tm 25.316667 0 Td 66.975309 Tz (8%\) ) Tj 1.438889 0 Td 52.391975 Tz (and/or ) Tj 2.1 0 Td 54.166667 Tz (calcium ) Tj 2.527778 0 Td 54.73251 Tz (phosphate ) Tj 3.188889 0 Td 52.314815 Tz (granule ) Tj 2.430556 0 Td 60.493827 Tz (Volume ) Tj 2.391667 0 Td 51.851852 Tz (percent ) Tj 2.391667 0 Td 54.012346 Tz (\(20 ) Tj 51.428571 0 0 51.428571 0 1888.332903 Tm 25.297222 0 Td 44.290123 Tz (vs. ) Tj 1.186111 0 Td 63.194444 Tz (10%\) ) Tj 1.788889 0 Td 55.555556 Tz (allowed ) Tj 2.605556 0 Td 48.611111 Tz (for ) Tj 1.127778 0 Td 51.851852 Tz (tuning ) Tj 2.177778 0 Td 48.611111 Tz (the ) Tj 1.186111 0 Td 52.662037 Tz (response ) Tj 2.819444 0 Td 49.907407 Tz (associated ) Tj 3.305556 0 Td 53.472222 Tz (with ) Tj 51.428571 0 0 51.428571 0 1850.419355 Tm 25.297222 0 Td 44.965278 Tz (platelet ) Tj 2.294444 0 Td 47.962963 Tz (releasate-associated ) Tj 5.852778 0 Td 56.712963 Tz (growth ) Tj 2.197222 0 Td 47.530864 Tz (factor ) Tj 1.788889 0 Td 45.775463 Tz (elution. ) Tj 2.333333 0 Td 50.231481 Tz (Tunabliity ) Tj 51.428571 0 0 51.428571 0 1813.5676 Tm 25.297222 0 Td 58.333333 Tz (from ) Tj 1.672222 0 Td 53.472222 Tz (completely ) Tj 3.461111 0 Td 52.146465 Tz (suppressing ) Tj 3.655556 0 Td 50.771605 Tz (the ) Tj 1.147222 0 Td 54.282407 Tz (inflammatory ) Tj 4.180556 0 Td 53.06713 Tz (response ) Tj 2.780556 0 Td 45.37037 Tz (to ) Tj 51.428571 0 0 51.428571 0 1771.948778 Tm 25.297222 0 Td 57.685185 Tz (augmenting ) Tj 3.538889 0 Td 49.691358 Tz (the ) Tj 1.030556 0 Td 52.662037 Tz (response ) Tj 2.644444 0 Td 61.574074 Tz (was ) Tj 1.244444 0 Td 54.282407 Tz (observed ) Tj 2.702778 0 Td 53.703704 Tz (through ) Tj 2.391667 0 Td 49.691358 Tz (varied ) Tj 1.866667 0 Td 49.074074 Tz (elution ) Tj 51.428571 0 0 51.428571 0 1731.98044 Tm 25.297222 0 Td 45.37037 Tz (profiles ) Tj 2.45 0 Td 59.953704 Tz (of ) Tj 0.894444 0 Td 53.472222 Tz (both ) Tj 1.594444 0 Td 48.229847 Tz (releasate-derived ) Tj 5.191667 0 Td 51.49177 Tz (bioagents ) Tj 3.052778 0 Td 59.41358 Tz (and ) Tj 1.322222 0 Td 49.259259 Tz (impurities ) Tj 51.428571 0 0 51.428571 0 1690.126092 Tm 25.297222 0 Td 49.826389 Tz (inherent ) Tj 2.625 0 Td 46.990741 Tz (to ) Tj 0.797222 0 Td 46.090535 Tz (alginate. ) Tj 2.722222 0 Td 87.5 Tz (A ) Tj 0.777778 0 Td 48.611111 Tz (2.5-fold ) Tj 2.566667 0 Td 59.953704 Tz (up ) Tj 1.011111 0 Td 47.962963 Tz (regulation ) Tj 3.15 0 Td 59.953704 Tz (of ) Tj 0.855556 0 Td 54.73251 Tz (inducible ) Tj 52.857143 0 0 52.857143 0 1647.887668 Tm 24.632432 0 Td 40.465465 Tz (nitric ) Tj 1.645946 0 Td 53.603604 Tz (oxide ) Tj 1.778378 0 Td 51.238739 Tz (synthase ) Tj 2.610811 0 Td 56.756757 Tz (gene ) Tj 1.551351 0 Td 50.45045 Tz (expression ) Tj 3.216216 0 Td 52.027027 Tz (followed ) Tj 2.686486 0 Td 56.756757 Tz (by ) Tj 0.87027 0 Td 56.756757 Tz (a ) Tj 0.567568 0 Td 49.099099 Tz (10-fold ) Tj 51.428571 0 0 51.428571 0 1604.745993 Tm 25.297222 0 Td 49.826389 Tz (increase ) Tj 2.566667 0 Td 45.37037 Tz (in ) Tj 0.758333 0 Td 41.203704 Tz (nitrite ) Tj 1.944444 0 Td 58.333333 Tz (media ) Tj 1.925 0 Td 45.910494 Tz (levels ) Tj 1.866667 0 Td 60.493827 Tz (was ) Tj 1.302778 0 Td 51.851852 Tz (inducted ) Tj 2.644444 0 Td 59.953704 Tz (by ) Tj 0.933333 0 Td 50.411523 Tz (including ) Tj 2.936111 0 Td 56.712963 Tz (of ) Tj 40 0 0 40 0 1569 Tm 32.525 0 Td 60.648148 Tz (releasate ) Tj 3.65 0 Td 65.972222 Tz (within ) Tj 2.8 0 Td 62.5 Tz (the ) Tj 1.5 0 Td 75 Tz (8 ) Tj 0.85 0 Td 79.166667 Tz (wt ) Tj 1.325 0 Td 83.333333 Tz (%/10 ) Tj 2.35 0 Td 65.277778 Tz (Vol ) Tj 1.6 0 Td 120.833333 Tz (% ) Tj 1.125 0 Td 68.055556 Tz (formation ) Tj 4.075 0 Td 75 Tz (and ) Tj 1.725 0 Td 80.555556 Tz (was ) Tj 51.428571 0 0 51.428571 0 1521.84466 Tm 25.297222 0 Td 56.712963 Tz (comparable ) Tj 3.577778 0 Td 46.990741 Tz (to ) Tj 0.777778 0 Td 56.712963 Tz (an ) Tj 0.875 0 Td 54.012346 Tz (endotoxin ) Tj 3.130556 0 Td 47.800926 Tz (positive ) Tj 2.508333 0 Td 45.775463 Tz (control. ) Tj 2.430556 0 Td 56.30787 Tz (Whereas, ) Tj 2.936111 0 Td 52.662037 Tz (near ) Tj 51.428571 0 0 51.428571 0 1479.309609 Tm 25.297222 0 Td 55.902778 Tz (complete ) Tj 2.8 0 Td 50.378788 Tz (elimination ) Tj 3.480556 0 Td 58.333333 Tz (of ) Tj 0.758333 0 Td 53.472222 Tz (inflammation ) Tj 4.063889 0 Td 60.493827 Tz (was ) Tj 1.263889 0 Td 52.662037 Tz (seen ) Tj 1.438889 0 Td 65.625 Tz (when ) Tj 1.75 0 Td 52.932099 Tz (releas ) Tj 40 0 0 40 0 1444 Tm 32.525 0 Td 61.111111 Tz (ate ) Tj 1.35 0 Td 77.777778 Tz (was ) Tj 1.675 0 Td 66.666667 Tz (included ) Tj 3.45 0 Td 65.277778 Tz (within ) Tj 2.625 0 Td 62.5 Tz (the ) Tj 1.35 0 Td 75 Tz (2 ) Tj 0.7 0 Td 79.166667 Tz (wt ) Tj 1.175 0 Td 84.375 Tz (%/20 ) Tj 2.25 0 Td 88.541667 Tz (vol% ) Tj 2.425 0 Td 63.541667 Tz (formulation. ) Tj 40 0 0 40 0 1403 Tm 33.45 0 Td 112.5 Tz (A ) Tj 1.125 0 Td 79.861111 Tz (number ) Tj 3.25 0 Td 72.916667 Tz (of ) Tj 1.175 0 Td 76.136364 Tz (embodiments ) Tj 5.45 0 Td 72.916667 Tz (of ) Tj 1.175 0 Td 63.888889 Tz (the ) Tj 1.55 0 Td 63.888889 Tz (invention ) Tj 3.875 0 Td 73.958333 Tz (have ) Tj 2.15 0 Td 75 Tz (been ) Tj 48.571429 0 0 48.571429 0 1355 Tm 26.785294 0 Td 51.813725 Tz (described. ) Tj 3.438235 0 Td 53.054299 Tz (Nevertheless, ) Tj 4.426471 0 Td 36.029412 Tz (it ) Tj 0.741176 0 Td 48.897059 Tz (will ) Tj 1.441176 0 Td 63.480392 Tz (be ) Tj 1.029412 0 Td 57.303922 Tz (understood ) Tj 3.747059 0 Td 47.181373 Tz (that ) Tj 1.441176 0 Td 52.941176 Tz (various ) Tj 51.428571 0 0 51.428571 0 1314.769231 Tm 25.297222 0 Td 50.605413 Tz (modifications ) Tj 4.219444 0 Td 66.975309 Tz (may ) Tj 1.419444 0 Td 58.333333 Tz (be ) Tj 0.933333 0 Td 52.314815 Tz (without ) Tj 2.45 0 Td 50.411523 Tz (departing ) Tj 2.936111 0 Td 58.333333 Tz (from ) Tj 1.613889 0 Td 50.771605 Tz (the ) Tj 1.147222 0 Td 41.589506 Tz (spirit ) Tj 1.672222 0 Td 60.493827 Tz (and ) Tj 51.428571 0 0 51.428571 0 1272.172524 Tm 25.316667 0 Td 55.092593 Tz (Scope ) Tj 1.808333 0 Td 56.712963 Tz (of ) Tj 0.777778 0 Td 50.771605 Tz (the ) Tj 1.108333 0 Td 47.314815 Tz (invention. ) Tj 27.142857 0 0 27.142857 0 747 Tm 58.542105 0 Td 95.175439 Tz (24 ) Tj 27.142857 0 0 27.142857 0 396 Tm 58.542105 0 Td 95.175439 Tz (24 ) Tj ET Q endstream endobj 478 0 obj << /Length 1 >> stream q endstream endobj 479 0 obj << /Length 29874 >> stream Q q 0.24 0 0 0.24 0 0 cm BT /F1 1 Tf 3 Tr 62.857143 0 0 62.857143 0 3023 Tm 17.325 0 Td 83.522727 Tz (US ) Tj 1.240909 0 Td 58.333333 Tz (8,404,256 ) Tj 3.388636 0 Td 74.242424 Tz (B2 ) Tj 52.857143 0 0 52.857143 0 2968 Tm 14.208108 0 Td 77.252252 Tz (29 ) Tj 52.857143 0 0 52.857143 0 2968 Tm 32.635135 0 Td 77.252252 Tz (30 ) Tj 38.571429 0 0 38.571429 0 2913 Tm 29.451852 0 Td 64.814815 Tz (- ) Tj 0.544444 0 Td 96.502058 Tz (Continued ) Tj 30 0 0 30 0 2852 Tm 10.933333 0 Td 94.444444 Tz (<213> ) Tj 3.5 0 Td 93.209877 Tz (ORGANISM: ) Tj 5.766667 0 Td 97.222222 Tz (Murine ) Tj 32.857143 0 0 32.857143 0 2781 Tm 9.982609 0 Td 81.15942 Tz (<4 ) Tj 1.095652 0 Td 62.137681 Tz (OOs, ) Tj 2.069565 0 Td 85.668277 Tz (SEQUENCE: ) Tj 5.326087 0 Td 71.014493 Tz (3 ) Tj 34.285714 0 0 34.285714 0 2717.352792 Tm 9.566667 0 Td 76.010101 Tz (tattgcticaa ) Tj 5.570833 0 Td 61.979167 Tz (tict ) Tj 1.575 0 Td 82.638889 Tz (cqggtgc ) Tj 4.025 0 Td 63.194444 Tz (tiga ) Tj 27.142857 0 0 27.142857 0 2619 Tm 12.084211 0 Td 99.473684 Tz (<21Os ) Tj 27.142857 0 0 27.142857 0 2585 Tm 12.084211 0 Td 101.315789 Tz (<211 ) Tj 2.763158 0 Td 67.54386 Tz (> ) Tj 32.857143 0 0 32.857143 0 2615 Tm 13.178261 0 Td 87.922705 Tz (SEQ ) Tj 2.16087 0 Td 81.15942 Tz (ID ) Tj 1.521739 0 Td 88.768116 Tz (NO ) Tj 1.613043 0 Td 71.014493 Tz (4 ) Tj 27.142857 0 0 27.142857 0 2585 Tm 15.952632 0 Td 101.754386 Tz (LENGTH: ) Tj 5.157895 0 Td 95.175439 Tz (24 ) Tj 27.142857 0 0 27.142857 0 2552 Tm 15.952632 0 Td 99.473684 Tz (TYPE: ) Tj 3.868421 0 Td 108.479532 Tz (DNA ) Tj 30 0 0 30 0 2519 Tm 14.4 0 Td 93.82716 Tz (ORGANISM: ) Tj 5.766667 0 Td 98.148148 Tz (Murine ) Tj 32.857143 0 0 32.857143 0 2448 Tm 9.982609 0 Td 81.15942 Tz (<4 ) Tj 1.095652 0 Td 62.137681 Tz (OOs, ) Tj 2.069565 0 Td 85.668277 Tz (SEQUENCE: ) Tj 5.356522 0 Td 65.942029 Tz (4 ) Tj 34.285714 0 0 34.285714 0 2385 Tm 9.566667 0 Td 83.611111 Tz (ccagagcgaa ) Tj 5.6 0 Td 83.611111 Tz (agcatttgcc ) Tj 5.6 0 Td 83.854167 Tz (aaga ) Tj 32.857143 0 0 32.857143 0 2282 Tm 13.178261 0 Td 86.231884 Tz (SEO ) Tj 2.191304 0 Td 78.623188 Tz (ID ) Tj 1.491304 0 Td 86.231884 Tz (NO ) Tj 1.643478 0 Td 71.014493 Tz (5 ) Tj 27.142857 0 0 27.142857 0 2253 Tm 15.952632 0 Td 101.754386 Tz (LENGTH: ) Tj 5.157895 0 Td 95.175439 Tz (24 ) Tj 27.142857 0 0 27.142857 0 2220 Tm 15.952632 0 Td 99.473684 Tz (TYPE: ) Tj 3.868421 0 Td 108.479532 Tz (DNA ) Tj 30 0 0 30 0 2186 Tm 14.4 0 Td 93.82716 Tz (ORGANISM: ) Tj 5.766667 0 Td 98.148148 Tz (Murine ) Tj 32.857143 0 0 32.857143 0 2116 Tm 9.982609 0 Td 81.15942 Tz (<4 ) Tj 1.095652 0 Td 62.137681 Tz (OOs, ) Tj 2.069565 0 Td 85.668277 Tz (SEQUENCE: ) Tj 5.356522 0 Td 71.014493 Tz (5 ) Tj 34.285714 0 0 34.285714 0 2048.265356 Tm 9.566667 0 Td 65.138889 Tz (actic ) Tj 2.041667 0 Td 82.638889 Tz (ctdctt ) Tj 3.529167 0 Td 83.449074 Tz (gctgat ) Tj 3.120833 0 Td 80.208333 Tz (coac ) Tj 2.479167 0 Td 83.854167 Tz (atct ) Tj 32.857143 0 0 32.857143 0 1950 Tm 13.178261 0 Td 87.922705 Tz (SEQ ) Tj 2.16087 0 Td 81.15942 Tz (ID ) Tj 1.521739 0 Td 88.768116 Tz (NO ) Tj 1.643478 0 Td 65.942029 Tz (6 ) Tj 27.142857 0 0 27.142857 0 1920 Tm 15.952632 0 Td 101.754386 Tz (LENGTH: ) Tj 5.157895 0 Td 95.175439 Tz (24 ) Tj 27.142857 0 0 27.142857 0 1887 Tm 15.952632 0 Td 99.473684 Tz (TYPE: ) Tj 3.868421 0 Td 108.479532 Tz (DNA ) Tj 30 0 0 30 0 1854 Tm 14.4 0 Td 93.82716 Tz (ORGANISM: ) Tj 5.766667 0 Td 98.148148 Tz (Murine ) Tj 32.857143 0 0 32.857143 0 1783 Tm 9.982609 0 Td 81.15942 Tz (<4 ) Tj 1.095652 0 Td 62.137681 Tz (OOs, ) Tj 2.069565 0 Td 85.668277 Tz (SEQUENCE: ) Tj 5.356522 0 Td 65.942029 Tz (6 ) Tj 34.285714 0 0 34.285714 0 1720 Tm 9.566667 0 Td 83.611111 Tz (tcgtaccaca ) Tj 5.570833 0 Td 76.45202 Tz (ggcattgttga ) Tj 5.6 0 Td 68.055556 Tz (tigga ) Tj 40 0 0 40 0 1581 Tm 9.125 0 Td 80.555556 Tz (The ) Tj 1.7 0 Td 63.888889 Tz (invention ) Tj 3.7 0 Td 70.238095 Tz (claimed ) Tj 3.2 0 Td 44.444444 Tz (is: ) Tj 51.428571 0 0 51.428571 0 1538.79397 Tm 7.116667 0 Td 40.509259 Tz (1. ) Tj 0.641667 0 Td 71.296296 Tz (An ) Tj 1.030556 0 Td 46.990741 Tz (injectable ) Tj 2.955556 0 Td 45.37037 Tz (in ) Tj 0.738889 0 Td 42.12963 Tz (situ ) Tj 1.166667 0 Td 51.851852 Tz (curing ) Tj 2.002778 0 Td 50.084175 Tz (biomaterial ) Tj 3.461111 0 Td 55.452675 Tz (composite ) Tj 3.111111 0 Td 66.435185 Tz (prod ) Tj 51.428571 0 0 51.428571 0 1499.366834 Tm 6.338889 0 Td 50.771605 Tz (uct ) Tj 1.147222 0 Td 64.00463 Tz (made ) Tj 1.691667 0 Td 61.574074 Tz (by ) Tj 0.952778 0 Td 55.092593 Tz (a ) Tj 0.505556 0 Td 51.851852 Tz (process ) Tj 2.391667 0 Td 54.444444 Tz (comprising ) Tj 3.422222 0 Td 50.771605 Tz (the ) Tj 1.108333 0 Td 47.962963 Tz (steps ) Tj 1.633333 0 Td 55.092593 Tz (of ) Tj 0.991667 0 Td 57.523148 Tz (admixing ) Tj 2.936111 0 Td 61.574074 Tz (an ) Tj 51.428571 0 0 51.428571 0 1459.319392 Tm 6.358333 0 Td 48.206019 Tz (alginate ) Tj 2.702778 0 Td 48.206019 Tz (solution ) Tj 2.702778 0 Td 53.472222 Tz (with ) Tj 1.730556 0 Td 55.812757 Tz (nonporous ) Tj 3.519444 0 Td 48.070988 Tz (B-tricalcium ) Tj 3.869444 0 Td 54.73251 Tz (phosphate ) Tj 51.428571 0 0 51.428571 0 1418.745283 Tm 6.358333 0 Td 49.494949 Tz (aggregates, ) Tj 3.402778 0 Td 46.990741 Tz (in ) Tj 0.680556 0 Td 58.333333 Tz (a ) Tj 0.486111 0 Td 44.722222 Tz (sufficient ) Tj 2.8 0 Td 60.493827 Tz (amount ) Tj 2.275 0 Td 48.611111 Tz (to ) Tj 0.719444 0 Td 51.851852 Tz (allow ) Tj 1.75 0 Td 47.530864 Tz (for ) Tj 0.933333 0 Td 53.009259 Tz (polymerization ) Tj 51.428571 0 0 51.428571 0 1379.818182 Tm 6.358333 0 Td 45.37037 Tz (after ) Tj 1.497222 0 Td 46.8107 Tz (injection ) Tj 2.722222 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 48.611111 Tz (said ) Tj 1.341667 0 Td 50.084175 Tz (biomaterial ) Tj 3.480556 0 Td 55.452675 Tz (composite ) Tj 3.130556 0 Td 53.240741 Tz (product ) Tj 2.391667 0 Td 48.611111 Tz (to ) Tj 0.758333 0 Td 58.333333 Tz (form ) Tj 51.428571 0 0 51.428571 0 1340.791045 Tm 6.358333 0 Td 51.851852 Tz (a ) Tj 0.486111 0 Td 53.877315 Tz (hydrogel ) Tj 2.761111 0 Td 54.552469 Tz (having ) Tj 2.158333 0 Td 56.712963 Tz (from ) Tj 1.536111 0 Td 57.87037 Tz (between ) Tj 2.683333 0 Td 56.712963 Tz (10 ) Tj 0.855556 0 Td 45.37037 Tz (to ) Tj 0.719444 0 Td 61.574074 Tz (20 ) Tj 0.894444 0 Td 50.925926 Tz (percent ) Tj 2.294444 0 Td 59.953704 Tz (by ) Tj 0.933333 0 Td 59.953704 Tz (volume ) Tj 51.428571 0 0 51.428571 0 1300.419355 Tm 6.358333 0 Td 58.333333 Tz (of ) Tj 0.913889 0 Td 47.800926 Tz (B-tricalcium ) Tj 3.655556 0 Td 51.527778 Tz (phosphate. ) Tj 51.428571 0 0 51.428571 0 1260.891821 Tm 7.097222 0 Td 42.12963 Tz (2. ) Tj 0.7 0 Td 64.814815 Tz (The ) Tj 1.322222 0 Td 53.703704 Tz (product ) Tj 2.469444 0 Td 56.712963 Tz (of ) Tj 0.777778 0 Td 53.148148 Tz (claim ) Tj 1.866667 0 Td 45.37037 Tz (1 ) Tj 0.544444 0 Td 54.62963 Tz (wherein ) Tj 2.508333 0 Td 49.421296 Tz (said ) Tj 1.380556 0 Td 51.203704 Tz (aggregates ) Tj 3.266667 0 Td 57.523148 Tz (have ) Tj 1.575 0 Td 55.092593 Tz (a ) Tj 51.428571 0 0 51.428571 0 1218 Tm 6.358333 0 Td 53.877315 Tz (diameter ) Tj 2.741667 0 Td 45.910494 Tz (distribution ) Tj 3.519444 0 Td 56.712963 Tz (of ) Tj 0.777778 0 Td 57.407407 Tz (between ) Tj 2.683333 0 Td 56.481481 Tz (125-150 ) Tj 2.547222 0 Td 44.560185 Tz (lum. ) Tj 51.428571 0 0 51.428571 0 1181.534351 Tm 7.097222 0 Td 42.12963 Tz (3. ) Tj 0.661111 0 Td 62.654321 Tz (The ) Tj 1.263889 0 Td 53.240741 Tz (product ) Tj 2.333333 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 53.148148 Tz (claim ) Tj 1.769444 0 Td 51.851852 Tz (1 ) Tj 0.466667 0 Td 56.018519 Tz (wherein ) Tj 2.488889 0 Td 48.611111 Tz (said ) Tj 1.283333 0 Td 58.333333 Tz (a ) Tj 0.447222 0 Td 54.282407 Tz (hydrogel ) Tj 2.722222 0 Td 54.012346 Tz (has ) Tj 1.127778 0 Td 57.523148 Tz (from ) Tj 51.428571 0 0 51.428571 0 1143.814159 Tm 6.338889 0 Td 57.87037 Tz (between ) Tj 2.625 0 Td 58.333333 Tz (2 ) Tj 0.563889 0 Td 43.75 Tz (to ) Tj 0.719444 0 Td 61.574074 Tz (8 ) Tj 0.583333 0 Td 54.552469 Tz (weight ) Tj 2.119444 0 Td 52.314815 Tz (percent ) Tj 2.333333 0 Td 58.333333 Tz (of ) Tj 0.797222 0 Td 45.37037 Tz (alginate. ) Tj 51.428571 0 0 51.428571 0 1103.873684 Tm 7.097222 0 Td 42.12963 Tz (4. ) Tj 0.7 0 Td 63.734568 Tz (The ) Tj 1.322222 0 Td 53.703704 Tz (product ) Tj 2.430556 0 Td 55.092593 Tz (of ) Tj 0.816667 0 Td 52.5 Tz (claim ) Tj 1.808333 0 Td 48.611111 Tz (1 ) Tj 0.525 0 Td 47.685185 Tz (further ) Tj 2.197222 0 Td 54.012346 Tz (comprises ) Tj 3.111111 0 Td 55.092593 Tz (adding ) Tj 2.177778 0 Td 55.092593 Tz (a ) Tj 0.525 0 Td 61.574074 Tz (Suf ) Tj 51.428571 0 0 51.428571 0 1060.820766 Tm 6.358333 0 Td 43.518519 Tz (ficient ) Tj 2.002778 0 Td 61.574074 Tz (amount ) Tj 2.411111 0 Td 58.333333 Tz (of ) Tj 0.816667 0 Td 54.62963 Tz (calcium ) Tj 2.527778 0 Td 45.833333 Tz (Sulfate ) Tj 2.119444 0 Td 46.990741 Tz (to ) Tj 0.797222 0 Td 48.611111 Tz (said ) Tj 1.516667 0 Td 47.530864 Tz (B-tricalcium ) Tj 3.655556 0 Td 67.24537 Tz (phos ) Tj 51.428571 0 0 51.428571 0 1023.979243 Tm 6.358333 0 Td 53.148148 Tz (phate ) Tj 1.75 0 Td 52.391975 Tz (before ) Tj 2.041667 0 Td 57.928241 Tz (admixing ) Tj 2.955556 0 Td 49.421296 Tz (said ) Tj 1.361111 0 Td 46.090535 Tz (solution, ) Tj 2.722222 0 Td 55.092593 Tz (wherein ) Tj 2.508333 0 Td 58.333333 Tz (a ) Tj 0.525 0 Td 45.37037 Tz (rate ) Tj 1.283333 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 64.814815 Tz (poly ) Tj 51.428571 0 0 51.428571 0 981.555891 Tm 6.358333 0 Td 51.527778 Tz (merization ) Tj 3.286111 0 Td 40.509259 Tz (is ) Tj 0.7 0 Td 50.411523 Tz (increased ) Tj 2.897222 0 Td 59.41358 Tz (and ) Tj 1.225 0 Td 58.333333 Tz (a ) Tj 0.505556 0 Td 49.691358 Tz (gel ) Tj 1.069444 0 Td 55.092593 Tz (time ) Tj 1.497222 0 Td 38.888889 Tz (is ) Tj 0.680556 0 Td 51.446759 Tz (lowered. ) Tj 51.428571 0 0 51.428571 0 945.879423 Tm 7.097222 0 Td 42.12963 Tz (5. ) Tj 0.719444 0 Td 62.654321 Tz (The ) Tj 1.302778 0 Td 53.703704 Tz (product ) Tj 2.430556 0 Td 56.712963 Tz (of ) Tj 0.797222 0 Td 53.148148 Tz (claim ) Tj 1.827778 0 Td 51.851852 Tz (1 ) Tj 0.544444 0 Td 47.222222 Tz (further ) Tj 2.138889 0 Td 54.73251 Tz (comprises ) Tj 3.15 0 Td 55.092593 Tz (adding ) Tj 2.177778 0 Td 51.851852 Tz (a ) Tj 0.505556 0 Td 62.654321 Tz (suf ) Tj 51.428571 0 0 51.428571 0 903.083434 Tm 6.358333 0 Td 43.518519 Tz (ficient ) Tj 2.002778 0 Td 61.574074 Tz (amount ) Tj 2.391667 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 45.37037 Tz (platelet ) Tj 2.352778 0 Td 47.530864 Tz (releasate ) Tj 2.741667 0 Td 46.990741 Tz (to ) Tj 0.797222 0 Td 48.611111 Tz (said ) Tj 1.361111 0 Td 46.090535 Tz (solution, ) Tj 2.702778 0 Td 56.481481 Tz (wherein ) Tj 51.428571 0 0 51.428571 0 866.679245 Tm 6.338889 0 Td 50.771605 Tz (the ) Tj 1.030556 0 Td 53.240741 Tz (product ) Tj 2.352778 0 Td 45.37037 Tz (in ) Tj 0.661111 0 Td 51.851852 Tz (vivo ) Tj 1.380556 0 Td 56.17284 Tz (can ) Tj 1.147222 0 Td 49.331276 Tz (stimulate ) Tj 2.741667 0 Td 44.965278 Tz (platelet ) Tj 2.313889 0 Td 46.8107 Tz (releasate ) Tj 2.644444 0 Td 44.290123 Tz (bioactivity. ) Tj 51.428571 0 0 51.428571 0 824.162907 Tm 7.116667 0 Td 40.509259 Tz (6. ) Tj 0.602778 0 Td 63.734568 Tz (The ) Tj 1.244444 0 Td 53.703704 Tz (product ) Tj 2.352778 0 Td 51.851852 Tz (of ) Tj 0.719444 0 Td 53.148148 Tz (claim ) Tj 1.711111 0 Td 64.814815 Tz (5 ) Tj 0.505556 0 Td 55.555556 Tz (wherein ) Tj 2.430556 0 Td 51.851852 Tz (a ) Tj 0.466667 0 Td 44.398148 Tz (sufficient ) Tj 2.761111 0 Td 61.033951 Tz (amount ) Tj 2.313889 0 Td 53.472222 Tz (of ) Tj 0.738889 0 Td 48.611111 Tz (the ) Tj 51.428571 0 0 51.428571 0 787.604819 Tm 6.358333 0 Td 44.965278 Tz (platelet ) Tj 2.313889 0 Td 46.8107 Tz (releasate ) Tj 2.644444 0 Td 52.777778 Tz (provide ) Tj 2.372222 0 Td 45.37037 Tz (at ) Tj 0.680556 0 Td 44.722222 Tz (least ) Tj 1.497222 0 Td 59.953704 Tz (50 ) Tj 0.836111 0 Td 51.388889 Tz (percent ) Tj 2.275 0 Td 61.574074 Tz (by ) Tj 0.913889 0 Td 59.953704 Tz (volume ) Tj 2.294444 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 752 Tm 6.338889 0 Td 51.131687 Tz (hydrogel. ) Tj 51.428571 0 0 51.428571 0 708.879423 Tm 7.097222 0 Td 42.12963 Tz (7. ) Tj 0.719444 0 Td 62.654321 Tz (The ) Tj 1.302778 0 Td 53.703704 Tz (product ) Tj 2.430556 0 Td 56.712963 Tz (of ) Tj 0.797222 0 Td 53.148148 Tz (claim ) Tj 1.827778 0 Td 51.851852 Tz (1 ) Tj 0.544444 0 Td 47.222222 Tz (further ) Tj 2.138889 0 Td 54.73251 Tz (comprises ) Tj 3.15 0 Td 55.092593 Tz (adding ) Tj 2.177778 0 Td 51.851852 Tz (a ) Tj 0.505556 0 Td 62.654321 Tz (suf ) Tj 51.428571 0 0 51.428571 0 668.727856 Tm 6.358333 0 Td 43.518519 Tz (ficient ) Tj 2.119444 0 Td 61.033951 Tz (amount ) Tj 2.488889 0 Td 56.712963 Tz (of ) Tj 0.875 0 Td 53.796296 Tz (alpha ) Tj 1.905556 0 Td 56.944444 Tz (hydroxy ) Tj 2.722222 0 Td 50.87963 Tz (carboxylic ) Tj 3.363889 0 Td 49.259259 Tz (acids ) Tj 1.808333 0 Td 51.851852 Tz (or ) Tj 0.894444 0 Td 42.777778 Tz (salts ) Tj 51.428571 0 0 51.428571 0 629.479769 Tm 6.338889 0 Td 51.851852 Tz (thereof ) Tj 2.255556 0 Td 46.990741 Tz (to ) Tj 0.777778 0 Td 49.421296 Tz (increase ) Tj 2.566667 0 Td 48.611111 Tz (the ) Tj 1.069444 0 Td 46.843434 Tz (flowability ) Tj 3.266667 0 Td 59.953704 Tz (of ) Tj 0.797222 0 Td 49.691358 Tz (the ) Tj 1.069444 0 Td 51.131687 Tz (hydrogel. ) Tj 51.428571 0 0 51.428571 0 587.371728 Tm 7.097222 0 Td 42.12963 Tz (8. ) Tj 0.719444 0 Td 62.654321 Tz (The ) Tj 1.302778 0 Td 53.703704 Tz (product ) Tj 2.430556 0 Td 59.953704 Tz (of ) Tj 0.797222 0 Td 53.148148 Tz (claim ) Tj 1.808333 0 Td 61.574074 Tz (7 ) Tj 0.583333 0 Td 54.62963 Tz (wherein ) Tj 2.527778 0 Td 48.611111 Tz (the ) Tj 1.05 0 Td 52.5 Tz (alpha ) Tj 1.75 0 Td 57.87037 Tz (hydroxy ) Tj 2.644444 0 Td 61.574074 Tz (car ) Tj 51.428571 0 0 51.428571 0 544.391534 Tm 6.338889 0 Td 52.314815 Tz (boxylic ) Tj 2.411111 0 Td 37.268519 Tz (is ) Tj 0.661111 0 Td 50.771605 Tz (trisodium ) Tj 2.955556 0 Td 40.914352 Tz (citrate. ) Tj 51.428571 0 0 51.428571 0 503.978947 Tm 7.097222 0 Td 42.12963 Tz (9. ) Tj 0.738889 0 Td 63.734568 Tz (The ) Tj 1.380556 0 Td 52.777778 Tz (product ) Tj 2.430556 0 Td 59.953704 Tz (of ) Tj 0.816667 0 Td 53.796296 Tz (claim ) Tj 1.886111 0 Td 51.851852 Tz (1 ) Tj 0.583333 0 Td 47.685185 Tz (further ) Tj 2.197222 0 Td 54.768519 Tz (comprising ) Tj 3.5 0 Td 51.851852 Tz (a ) Tj 0.544444 0 Td 49.331276 Tz (bioactive ) Tj 45.714286 0 0 45.714286 0 466 Tm 7.153125 0 Td 54.079861 Tz (agent. ) Tj 51.428571 0 0 51.428571 0 420.062827 Tm 7.136111 0 Td 46.450617 Tz (10. ) Tj 1.030556 0 Td 63.734568 Tz (The ) Tj 1.341667 0 Td 53.240741 Tz (product ) Tj 2.411111 0 Td 59.953704 Tz (of ) Tj 0.816667 0 Td 52.5 Tz (claim ) Tj 1.769444 0 Td 64.814815 Tz (9 ) Tj 0.602778 0 Td 55.555556 Tz (wherein ) Tj 2.527778 0 Td 50.771605 Tz (the ) Tj 1.088889 0 Td 48.971193 Tz (bioactive ) Tj 2.838889 0 Td 53.148148 Tz (agent ) Tj 1.788889 0 Td 38.888889 Tz (is ) Tj 51.428571 0 0 51.428571 0 379.478685 Tm 6.358333 0 Td 51.388889 Tz (derived ) Tj 2.352778 0 Td 57.523148 Tz (from ) Tj 1.613889 0 Td 44.650206 Tz (platelets ) Tj 2.586111 0 Td 60.493827 Tz (and ) Tj 1.263889 0 Td 49.421296 Tz (said ) Tj 1.361111 0 Td 49.331276 Tz (bioactive ) Tj 2.838889 0 Td 53.148148 Tz (agent ) Tj 1.788889 0 Td 54.372428 Tz (comprises ) Tj 3.130556 0 Td 45.37037 Tz (at ) Tj 51.428571 0 0 51.428571 0 339.026076 Tm 6.358333 0 Td 44.722222 Tz (least ) Tj 1.633333 0 Td 59.953704 Tz (50 ) Tj 0.972222 0 Td 50.925926 Tz (percent ) Tj 2.391667 0 Td 59.953704 Tz (by ) Tj 1.05 0 Td 59.41358 Tz (volume ) Tj 2.391667 0 Td 59.953704 Tz (of ) Tj 0.875 0 Td 49.691358 Tz (the ) Tj 1.166667 0 Td 53.472222 Tz (hydrogel ) Tj 2.858333 0 Td 49.537037 Tz (portion ) Tj 2.372222 0 Td 58.333333 Tz (of ) Tj 0.875 0 Td 49.691358 Tz (the ) Tj 51.428571 0 0 51.428571 0 301 Tm 6.358333 0 Td 49.421296 Tz (product. ) Tj 51.428571 0 0 51.428571 0 253.838371 Tm 7.136111 0 Td 46.450617 Tz (11. ) Tj 1.030556 0 Td 63.734568 Tz (The ) Tj 1.341667 0 Td 53.240741 Tz (product ) Tj 2.430556 0 Td 58.333333 Tz (of ) Tj 0.797222 0 Td 52.5 Tz (claim ) Tj 1.769444 0 Td 64.814815 Tz (9 ) Tj 0.602778 0 Td 55.555556 Tz (wherein ) Tj 2.547222 0 Td 48.611111 Tz (said ) Tj 1.4 0 Td 55.812757 Tz (nonporous ) Tj 3.363889 0 Td 46.666667 Tz (B-tri ) Tj 51.428571 0 0 51.428571 0 212.915888 Tm 6.358333 0 Td 54.166667 Tz (calcium ) Tj 2.45 0 Td 54.73251 Tz (phosphate ) Tj 3.052778 0 Td 51.851852 Tz (aggregates ) Tj 3.266667 0 Td 56.712963 Tz (have ) Tj 1.458333 0 Td 58.333333 Tz (a ) Tj 0.466667 0 Td 53.877315 Tz (diameter ) Tj 2.683333 0 Td 46.180556 Tz (distribution ) Tj 3.5 0 Td 56.712963 Tz (of ) Tj 50 0 0 50 0 168 Tm 6.6 0 Td 58.095238 Tz (125-150 ) Tj 2.68 0 Td 58.888889 Tz (um. ) Tj 30 0 0 30 0 1507 Tm 41.6 0 Td 77.777778 Tz (35 ) Tj 30 0 0 30 0 1299 Tm 41.566667 0 Td 83.333333 Tz (40 ) Tj 30 0 0 30 0 1091 Tm 41.566667 0 Td 80.555556 Tz (45 ) Tj 30 0 0 30 0 883 Tm 41.6 0 Td 80.555556 Tz (50 ) Tj 30 0 0 30 0 675 Tm 41.6 0 Td 77.777778 Tz (55 ) Tj 30 0 0 30 0 467 Tm 41.633333 0 Td 77.777778 Tz (60 ) Tj 30 0 0 30 0 259 Tm 41.633333 0 Td 75 Tz (65 ) Tj 27.142857 0 0 27.142857 0 2719 Tm 57.142105 0 Td 98.245614 Tz (23 ) Tj 27.142857 0 0 27.142857 0 2386 Tm 57.142105 0 Td 98.245614 Tz (24 ) Tj 27.142857 0 0 27.142857 0 2053 Tm 57.142105 0 Td 98.245614 Tz (24 ) Tj 27.142857 0 0 27.142857 0 1721 Tm 57.142105 0 Td 98.245614 Tz (24 ) Tj 51.428571 0 0 51.428571 0 1575.550576 Tm 26.055556 0 Td 48.611111 Tz (12. ) Tj 1.011111 0 Td 64.814815 Tz (The ) Tj 1.302778 0 Td 53.703704 Tz (product ) Tj 2.391667 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 53.796296 Tz (claim ) Tj 1.769444 0 Td 64.814815 Tz (9 ) Tj 0.563889 0 Td 55.555556 Tz (wherein ) Tj 2.527778 0 Td 49.691358 Tz (the ) Tj 1.05 0 Td 53.877315 Tz (hydrogel ) Tj 2.761111 0 Td 54.012346 Tz (has ) Tj 1.127778 0 Td 58.333333 Tz (from ) Tj 51.428571 0 0 51.428571 0 1534.8 Tm 25.277778 0 Td 57.87037 Tz (between ) Tj 2.644444 0 Td 55.092593 Tz (2 ) Tj 0.525 0 Td 45.37037 Tz (to ) Tj 0.738889 0 Td 58.333333 Tz (8 ) Tj 0.544444 0 Td 55.632716 Tz (weight ) Tj 2.158333 0 Td 51.388889 Tz (percent ) Tj 2.333333 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 48.206019 Tz (alginate ) Tj 2.488889 0 Td 58.333333 Tz (and ) Tj 1.225 0 Td 50.771605 Tz (the ) Tj 1.088889 0 Td 55.092593 Tz (composite ) Tj 51.428571 0 0 51.428571 0 1488.755319 Tm 25.277778 0 Td 54.012346 Tz (has ) Tj 1.283333 0 Td 57.523148 Tz (from ) Tj 1.672222 0 Td 57.87037 Tz (between ) Tj 2.8 0 Td 56.712963 Tz (10 ) Tj 0.933333 0 Td 51.388889 Tz (percent ) Tj 2.430556 0 Td 45.37037 Tz (to ) Tj 0.875 0 Td 61.574074 Tz (20 ) Tj 0.991667 0 Td 51.388889 Tz (percent ) Tj 2.411111 0 Td 59.953704 Tz (by ) Tj 1.069444 0 Td 59.41358 Tz (volume ) Tj 2.411111 0 Td 58.333333 Tz (of ) Tj 51.428571 0 0 51.428571 0 1446.79822 Tm 25.433333 0 Td 47.800926 Tz (B-tricalcium ) Tj 3.636111 0 Td 51.851852 Tz (phosphate. ) Tj 51.428571 0 0 51.428571 0 1403.391574 Tm 26.036111 0 Td 48.611111 Tz (13. ) Tj 0.972222 0 Td 90.740741 Tz (A ) Tj 0.661111 0 Td 43.209877 Tz (kit ) Tj 0.894444 0 Td 52.146465 Tz (comprising: ) Tj 3.597222 0 Td 55.092593 Tz (a ) Tj 0.466667 0 Td 37.592593 Tz (first ) Tj 1.244444 0 Td 57.79321 Tz (mixing ) Tj 2.197222 0 Td 50.411523 Tz (container ) Tj 2.819444 0 Td 51.527778 Tz (containing ) Tj 3.188889 0 Td 58.333333 Tz (an ) Tj 51.428571 0 0 51.428571 0 1358.958549 Tm 25.297222 0 Td 48.206019 Tz (alginate ) Tj 2.722222 0 Td 46.450617 Tz (solution, ) Tj 2.916667 0 Td 55.092593 Tz (a ) Tj 0.758333 0 Td 55.632716 Tz (second ) Tj 2.430556 0 Td 56.712963 Tz (mixing ) Tj 2.45 0 Td 50.771605 Tz (container ) Tj 3.111111 0 Td 51.203704 Tz (containing ) Tj 51.428571 0 0 51.428571 0 1314.355954 Tm 25.277778 0 Td 57.87037 Tz (between ) Tj 2.761111 0 Td 56.712963 Tz (10 ) Tj 0.913889 0 Td 45.37037 Tz (to ) Tj 0.797222 0 Td 61.574074 Tz (20 ) Tj 0.952778 0 Td 51.388889 Tz (percent ) Tj 2.391667 0 Td 58.333333 Tz (by ) Tj 0.972222 0 Td 59.953704 Tz (volume ) Tj 2.391667 0 Td 59.953704 Tz (of ) Tj 0.972222 0 Td 47.800926 Tz (B-tricalcium ) Tj 3.713889 0 Td 67.24537 Tz (phos ) Tj 51.428571 0 0 51.428571 0 1269.962073 Tm 25.297222 0 Td 48.611111 Tz (phate, ) Tj 2.022222 0 Td 55.092593 Tz (wherein ) Tj 2.586111 0 Td 49.421296 Tz (said ) Tj 1.458333 0 Td 36.944444 Tz (first ) Tj 1.4 0 Td 56.17284 Tz (mixing ) Tj 2.294444 0 Td 50.411523 Tz (container ) Tj 2.955556 0 Td 59.41358 Tz (and ) Tj 1.302778 0 Td 48.611111 Tz (said ) Tj 1.419444 0 Td 56.712963 Tz (second ) Tj 51.428571 0 0 51.428571 0 1226.274214 Tm 25.297222 0 Td 57.253086 Tz (mixing ) Tj 2.372222 0 Td 50.05144 Tz (container ) Tj 2.955556 0 Td 50.771605 Tz (are ) Tj 1.186111 0 Td 54.73251 Tz (connected ) Tj 3.227778 0 Td 58.333333 Tz (and ) Tj 1.361111 0 Td 54.62963 Tz (wherein ) Tj 2.625 0 Td 47.800926 Tz (said ) Tj 1.419444 0 Td 48.611111 Tz (alginate ) Tj 51.428571 0 0 51.428571 0 1184.188406 Tm 25.316667 0 Td 48.206019 Tz (Solution ) Tj 2.469444 0 Td 58.333333 Tz (and ) Tj 1.186111 0 Td 48.611111 Tz (said ) Tj 1.438889 0 Td 48.070988 Tz (B-tricalcium ) Tj 3.597222 0 Td 54.73251 Tz (phosphate ) Tj 3.091667 0 Td 59.953704 Tz (upon ) Tj 1.633333 0 Td 56.17284 Tz (mixing ) Tj 2.177778 0 Td 56.712963 Tz (form ) Tj 1.536111 0 Td 55.092593 Tz (a ) Tj 51.428571 0 0 51.428571 0 1139.35533 Tm 25.277778 0 Td 51.49177 Tz (hydrogel; ) Tj 2.994444 0 Td 58.333333 Tz (and ) Tj 1.225 0 Td 55.092593 Tz (a ) Tj 0.544444 0 Td 51.851852 Tz (needle ) Tj 2.080556 0 Td 55.092593 Tz (of ) Tj 0.797222 0 Td 50.771605 Tz (not 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Td 67.261905 Tz (extrude ) Tj 51.428571 0 0 51.428571 0 1007.595166 Tm 25.316667 0 Td 48.611111 Tz (said ) Tj 1.341667 0 Td 53.472222 Tz (hydrogel ) Tj 2.761111 0 Td 57.523148 Tz (from ) Tj 1.594444 0 Td 48.611111 Tz (said ) Tj 1.361111 0 Td 37.592593 Tz (first ) Tj 1.302778 0 Td 53.472222 Tz (or ) Tj 0.797222 0 Td 56.17284 Tz (second ) Tj 2.216667 0 Td 46.666667 Tz (container. ) Tj 51.428571 0 0 51.428571 0 964.888011 Tm 26.055556 0 Td 48.611111 Tz (14. ) Tj 1.05 0 Td 63.734568 Tz (The ) Tj 1.361111 0 Td 42.12963 Tz (kit ) Tj 0.952778 0 Td 58.333333 Tz (of ) Tj 0.797222 0 Td 53.148148 Tz (claim ) Tj 1.847222 0 Td 55.092593 Tz (13 ) Tj 0.894444 0 Td 47.685185 Tz (further ) Tj 2.177778 0 Td 54.768519 Tz (comprising ) Tj 3.461111 0 Td 51.851852 Tz (a ) Tj 0.525 0 Td 46.018519 Tz (third ) Tj 1.594444 0 Td 56.712963 Tz (mixing ) Tj 48.571429 0 0 48.571429 0 921 Tm 26.785294 0 Td 50.441176 Tz (container, ) Tj 3.252941 0 Td 52.5 Tz (attachable ) Tj 3.376471 0 Td 51.470588 Tz (to ) Tj 0.864706 0 Td 50.326797 Tz (either ) Tj 2.038235 0 Td 52.614379 Tz (the ) Tj 1.194118 0 Td 39.803922 Tz (first ) Tj 1.441176 0 Td 56.617647 Tz (or ) Tj 0.926471 0 Td 58.905229 Tz (second ) Tj 2.367647 0 Td 50.098039 Tz (container, ) Tj 51.428571 0 0 51.428571 0 877.289389 Tm 25.277778 0 Td 54.282407 Tz (with ) Tj 1.477778 0 Td 58.333333 Tz (a ) Tj 0.505556 0 Td 49.331276 Tz (bioactive ) Tj 2.838889 0 Td 48.070988 Tz (agent. ) Tj 51.428571 0 0 51.428571 0 835.646075 Tm 26.055556 0 Td 48.611111 Tz (15. ) Tj 1.030556 0 Td 87.5 Tz (A ) Tj 0.777778 0 Td 59.41358 Tz (method ) Tj 2.352778 0 Td 45.37037 Tz (to ) Tj 0.777778 0 Td 42.12963 Tz (facilitate ) Tj 2.722222 0 Td 59.143519 Tz (bone ) Tj 1.633333 0 Td 47.530864 Tz (repair ) Tj 1.866667 0 Td 54.768519 Tz (comprising ) Tj 3.461111 0 Td 69.351852 Tz (admin ) Tj 51.428571 0 0 51.428571 0 795.057644 Tm 25.297222 0 Td 45.37037 Tz (istering ) Tj 2.352778 0 Td 46.990741 Tz (to ) Tj 0.758333 0 Td 58.333333 Tz (a ) Tj 0.544444 0 Td 63.014403 Tz 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51.428571 0 0 51.428571 0 2913.976422 Tm 7.097222 0 Td 49.691358 Tz (21. ) Tj 1.166667 0 Td 62.654321 Tz (The ) Tj 1.438889 0 Td 59.953704 Tz (method ) Tj 2.391667 0 Td 61.574074 Tz (of ) Tj 0.913889 0 Td 53.148148 Tz (claim ) Tj 1.925 0 Td 56.712963 Tz (15 ) Tj 0.972222 0 Td 55.092593 Tz (wherein ) Tj 2.625 0 Td 48.611111 Tz (the ) Tj 1.166667 0 Td 47.530864 Tz (repair ) Tj 1.944444 0 Td 40.509259 Tz (is ) Tj 0.758333 0 Td 59.953704 Tz (bone ) Tj 51.428571 0 0 51.428571 0 2876.864671 Tm 6.358333 0 Td 53.030303 Tz (remodeling, ) Tj 3.694444 0 Td 48.611111 Tz (similar ) Tj 2.197222 0 Td 45.37037 Tz (to ) Tj 0.738889 0 Td 58.130787 Tz (hematoma-induced ) Tj 5.775 0 Td 46.990741 Tz (fracture ) Tj 2.411111 0 Td 48.206019 Tz (healing. ) Tj 51.428571 0 0 51.428571 0 2836.629012 Tm 7.097222 0 Td 48.611111 Tz (22. ) Tj 1.088889 0 Td 84.259259 Tz (A ) Tj 0.719444 0 Td 59.953704 Tz (method ) Tj 2.352778 0 Td 46.990741 Tz (to ) Tj 0.777778 0 Td 42.777778 Tz (facilitate ) Tj 2.741667 0 Td 59.143519 Tz (bone ) Tj 1.633333 0 Td 47.530864 Tz (repair ) Tj 1.886111 0 Td 54.444444 Tz (comprising ) Tj 3.441667 0 Td 69.351852 Tz (admin ) Tj 51.428571 0 0 51.428571 0 2798.064677 Tm 6.358333 0 Td 45.775463 Tz (istering ) Tj 2.352778 0 Td 48.611111 Tz (to ) Tj 0.758333 0 Td 58.333333 Tz (a ) Tj 0.544444 0 Td 63.014403 Tz (mammalian ) Tj 3.616667 0 Td 49.537037 Tz (Subject ) Tj 2.275 0 Td 43.75 Tz (in ) Tj 0.777778 0 Td 55.902778 Tz (need ) Tj 1.536111 0 Td 56.712963 Tz (of ) Tj 0.797222 0 Td 54.282407 Tz (Such ) Tj 1.477778 0 Td 60.763889 Tz (bone ) Tj 1.652778 0 Td 47.530864 Tz (repair ) Tj 51.428571 0 0 51.428571 0 2756.755635 Tm 6.338889 0 Td 51.131687 Tz (treatment ) Tj 2.877778 0 Td 56.712963 Tz (an ) Tj 0.797222 0 Td 46.450617 Tz (effective ) Tj 2.605556 0 Td 60.493827 Tz (amount ) Tj 2.313889 0 Td 53.472222 Tz (of ) Tj 0.719444 0 Td 48.611111 Tz (the ) Tj 0.991667 0 Td 54.503367 Tz (composition ) Tj 3.713889 0 Td 51.851852 Tz (of ) Tj 0.719444 0 Td 52.5 Tz (claim ) Tj 1.672222 0 Td 64.814815 Tz (9 ) Tj 0.505556 0 Td 46.990741 Tz (to ) Tj 51.428571 0 0 51.428571 0 2719.360595 Tm 6.358333 0 Td 55.092593 Tz (a ) Tj 0.505556 0 Td 42.12963 Tz (site ) Tj 1.205556 0 Td 45.37037 Tz (in ) Tj 0.758333 0 Td 47.800926 Tz (said ) Tj 1.341667 0 Td 45.775463 Tz (Subject. ) Tj 40 0 0 40 0 2684 Tm 9.125 0 Td 62.5 Tz (23. ) Tj 1.425 0 Td 81.944444 Tz (The ) Tj 1.775 0 Td 77.083333 Tz (method ) Tj 3.05 0 Td 75 Tz (of ) Tj 1.05 0 Td 69.166667 Tz (claim ) Tj 2.35 0 Td 79.166667 Tz (22 ) Tj 1.25 0 Td 71.428571 Tz (wherein ) Tj 3.325 0 Td 62.5 Tz (said ) Tj 1.775 0 Td 54.166667 Tz (site ) Tj 1.575 0 Td 52.083333 Tz (is ) Tj 0.925 0 Td 63.541667 Tz (selected ) Tj 51.428571 0 0 51.428571 0 2639.258824 Tm 6.358333 0 Td 58.333333 Tz (from ) Tj 1.516667 0 Td 50.771605 Tz (the ) Tj 1.011111 0 Td 56.388889 Tz (group ) Tj 1.827778 0 Td 49.583333 Tz (consisting ) Tj 3.091667 0 Td 53.472222 Tz (of ) Tj 0.913889 0 Td 51.851852 Tz (Subchondral, ) Tj 3.85 0 Td 52.350427 Tz (osteochondral ) Tj 4.2 0 Td 60.493827 Tz (and ) Tj 40 0 0 40 0 2605 Tm 8.175 0 Td 65.416667 Tz (cancellous ) Tj 4.175 0 Td 67.5 Tz (bone. ) Tj 51.428571 0 0 51.428571 0 2559.298701 Tm 7.097222 0 Td 49.691358 Tz (24. ) Tj 1.05 0 Td 63.734568 Tz (The ) Tj 1.361111 0 Td 59.41358 Tz (method ) Tj 2.294444 0 Td 59.953704 Tz (of ) Tj 0.797222 0 Td 53.148148 Tz (claim ) Tj 1.808333 0 Td 56.712963 Tz (23 ) Tj 0.875 0 Td 55.555556 Tz (wherein ) Tj 2.527778 0 Td 48.611111 Tz (said ) Tj 1.341667 0 Td 59.143519 Tz (bone ) Tj 1.613889 0 Td 37.268519 Tz (is ) Tj 0.641667 0 Td 50.636574 Tz (adjacent ) Tj 51.428571 0 0 51.428571 0 2520.321951 Tm 6.338889 0 Td 46.990741 Tz (to ) Tj 0.719444 0 Td 58.333333 Tz (a ) Tj 0.486111 0 Td 47.530864 Tz (structure ) Tj 2.702778 0 Td 49.016204 Tz (selected ) Tj 2.508333 0 Td 57.523148 Tz (from ) Tj 1.555556 0 Td 49.691358 Tz (the ) Tj 1.030556 0 Td 56.388889 Tz (group ) Tj 1.866667 0 Td 48.935185 Tz (consisting ) Tj 3.111111 0 Td 53.472222 Tz (of ) Tj 0.933333 0 Td 47.530864 Tz (Vertebral ) Tj 51.428571 0 0 51.428571 0 2479.879499 Tm 6.358333 0 Td 50.771605 Tz (endplates ) Tj 2.936111 0 Td 58.333333 Tz (and ) Tj 1.205556 0 Td 54.166667 Tz (annulus ) Tj 2.488889 0 Td 47.395833 Tz (fibrosus ) Tj 2.469444 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 58.333333 Tz (a ) Tj 0.525 0 Td 48.070988 Tz (spinal ) Tj 1.925 0 Td 44.074074 Tz (disc. ) Tj 51.428571 0 0 51.428571 0 2436.316327 Tm 7.097222 0 Td 49.691358 Tz (25. ) Tj 1.011111 0 Td 63.734568 Tz (The ) Tj 1.283333 0 Td 60.493827 Tz (method ) Tj 2.313889 0 Td 53.472222 Tz (of ) Tj 0.719444 0 Td 53.148148 Tz (claim ) Tj 1.75 0 Td 59.953704 Tz (22 ) Tj 0.894444 0 Td 54.62963 Tz (wherein ) Tj 2.45 0 Td 48.611111 Tz (said ) Tj 1.322222 0 Td 41.319444 Tz (site ) Tj 1.147222 0 Td 37.268519 Tz (is ) Tj 0.602778 0 Td 46.450617 Tz (cartilage ) Tj 2.605556 0 Td 55.092593 Tz (or ) Tj 51.428571 0 0 51.428571 0 2401.175258 Tm 6.358333 0 Td 50.636574 Tz (adjacent ) Tj 2.586111 0 Td 48.611111 Tz (to ) Tj 0.777778 0 Td 44.074074 Tz (cartilage. ) Tj 40 0 0 40 0 2360 Tm 9.125 0 Td 62.5 Tz (26. ) Tj 1.425 0 Td 81.944444 Tz (The ) Tj 1.775 0 Td 77.083333 Tz (method ) Tj 3.05 0 Td 75 Tz (of ) Tj 1.05 0 Td 69.166667 Tz (claim ) Tj 2.35 0 Td 79.166667 Tz (22 ) Tj 1.25 0 Td 71.428571 Tz (wherein ) Tj 3.325 0 Td 62.5 Tz (said ) Tj 1.775 0 Td 54.166667 Tz (site ) Tj 1.575 0 Td 52.083333 Tz (is ) Tj 0.925 0 Td 63.541667 Tz (selected ) Tj 51.428571 0 0 51.428571 0 2314.575835 Tm 6.358333 0 Td 58.333333 Tz (from ) Tj 1.594444 0 Td 49.691358 Tz (the ) Tj 1.069444 0 Td 56.388889 Tz (group ) Tj 1.905556 0 Td 48.935185 Tz (consisting ) Tj 3.130556 0 Td 56.712963 Tz (of ) Tj 0.777778 0 Td 45.37037 Tz (articular ) Tj 2.605556 0 Td 58.333333 Tz (and ) Tj 1.225 0 Td 44.722222 Tz (fibrocartilage. ) Tj 51.428571 0 0 51.428571 0 2270.46383 Tm 7.097222 0 Td 49.691358 Tz (27. ) Tj 1.205556 0 Td 64.814815 Tz (The ) Tj 1.536111 0 Td 59.41358 Tz (method ) Tj 2.469444 0 Td 58.333333 Tz (of ) Tj 0.933333 0 Td 53.796296 Tz (claim ) Tj 2.002778 0 Td 58.333333 Tz (25 ) Tj 1.069444 0 Td 55.092593 Tz (wherein ) Tj 2.683333 0 Td 49.421296 Tz (said ) Tj 1.497222 0 Td 46.450617 Tz (cartilage ) Tj 2.838889 0 Td 40.509259 Tz (is ) Tj 51.428571 0 0 51.428571 0 2230.284635 Tm 6.377778 0 Td 48.611111 Tz (selected ) Tj 2.527778 0 Td 58.333333 Tz (from ) Tj 1.613889 0 Td 50.771605 Tz (the ) Tj 1.088889 0 Td 57.037037 Tz (group ) Tj 1.944444 0 Td 49.259259 Tz (consisting ) Tj 3.169444 0 Td 53.472222 Tz (of ) Tj 1.011111 0 Td 55.740741 Tz (upper ) Tj 1.847222 0 Td 58.333333 Tz (and ) Tj 1.263889 0 Td 55.092593 Tz (lower ) Tj 1.866667 0 Td 61.574074 Tz (ver ) Tj 51.428571 0 0 51.428571 0 2188.376135 Tm 6.338889 0 Td 46.450617 Tz (tebral ) Tj 1.866667 0 Td 50.411523 Tz (endplates ) Tj 2.916667 0 Td 59.41358 Tz (and ) Tj 1.225 0 Td 50.771605 Tz (the ) Tj 1.069444 0 Td 54.166667 Tz (annulus ) Tj 2.488889 0 Td 46.990741 Tz (fibrosus ) Tj 2.45 0 Td 58.333333 Tz (of ) Tj 0.777778 0 Td 58.333333 Tz (a ) Tj 0.525 0 Td 48.070988 Tz (spinal ) Tj 1.944444 0 Td 43.425926 Tz (disc. ) Tj 51.428571 0 0 51.428571 0 2144.975069 Tm 7.097222 0 Td 49.691358 Tz (28. ) Tj 1.166667 0 Td 62.654321 Tz (The ) Tj 1.438889 0 Td 59.953704 Tz (method ) Tj 2.391667 0 Td 61.574074 Tz (of ) Tj 0.913889 0 Td 53.148148 Tz (claim ) Tj 1.905556 0 Td 58.333333 Tz (22 ) Tj 0.991667 0 Td 55.092593 Tz (wherein ) Tj 2.625 0 Td 48.611111 Tz (the ) Tj 1.166667 0 Td 47.530864 Tz (repair ) Tj 1.944444 0 Td 40.509259 Tz (is ) Tj 0.758333 0 Td 59.953704 Tz (bone ) Tj 51.428571 0 0 51.428571 0 2104.864671 Tm 6.358333 0 Td 53.030303 Tz (remodeling, ) Tj 3.694444 0 Td 48.611111 Tz (similar ) Tj 2.197222 0 Td 45.37037 Tz (to ) Tj 0.738889 0 Td 58.130787 Tz (hematoma-induced ) Tj 5.775 0 Td 46.990741 Tz (fracture ) Tj 2.411111 0 Td 48.206019 Tz (healing. ) Tj 51.428571 0 0 51.428571 0 2063.234957 Tm 7.097222 0 Td 48.611111 Tz (29. ) Tj 1.147222 0 Td 84.259259 Tz (A ) Tj 0.777778 0 Td 51.851852 Tz (process ) Tj 2.430556 0 Td 48.611111 Tz (to ) Tj 0.855556 0 Td 48.611111 Tz (release ) Tj 2.275 0 Td 58.333333 Tz (a ) Tj 0.602778 0 Td 49.331276 Tz (bioactive ) Tj 2.897222 0 Td 53.796296 Tz (agent ) Tj 1.847222 0 Td 48.611111 Tz (to ) Tj 0.855556 0 Td 55.092593 Tz (a ) Tj 0.622222 0 Td 41.319444 Tz (site ) Tj 1.283333 0 Td 43.75 Tz (in ) Tj 0.816667 0 Td 55.092593 Tz (a ) Tj 51.428571 0 0 51.428571 0 2023.661118 Tm 6.377778 0 Td 49.074074 Tz (Subject ) Tj 2.236111 0 Td 52.146465 Tz (comprising: ) Tj 3.636111 0 Td 51.602564 Tz (administering ) Tj 4.2 0 Td 55.092593 Tz (an ) Tj 0.836111 0 Td 46.8107 Tz (effective ) Tj 2.683333 0 Td 61.574074 Tz (amount ) Tj 2.391667 0 Td 56.712963 Tz (of ) Tj 0.758333 0 Td 61.574074 Tz (an ) Tj 51.428571 0 0 51.428571 0 1981.925466 Tm 6.358333 0 Td 47.314815 Tz (injectable ) Tj 3.033333 0 Td 45.37037 Tz (in ) Tj 0.777778 0 Td 42.939815 Tz (situ ) Tj 1.263889 0 Td 51.311728 Tz (curing ) Tj 2.041667 0 Td 50.084175 Tz (biomaterial ) Tj 3.519444 0 Td 55.452675 Tz (composite ) Tj 3.169444 0 Td 48.611111 Tz (to ) Tj 0.777778 0 Td 50.771605 Tz (the ) Tj 1.108333 0 Td 42.12963 Tz (site ) Tj 1.225 0 Td 48.611111 Tz (in ) Tj 51.428571 0 0 51.428571 0 1941.57037 Tm 6.338889 0 Td 50.771605 Tz (the ) Tj 1.147222 0 Td 46.180556 Tz (Subject, ) Tj 2.430556 0 Td 49.421296 Tz (said ) Tj 1.438889 0 Td 48.611111 Tz (material ) Tj 2.527778 0 Td 53.877315 Tz (prepared ) Tj 2.780556 0 Td 59.953704 Tz (by ) Tj 0.933333 0 Td 58.333333 Tz (a ) Tj 0.544444 0 Td 51.851852 Tz (process ) Tj 2.430556 0 Td 54.444444 Tz (comprising ) Tj 51.428571 0 0 51.428571 0 1898.378003 Tm 6.338889 0 Td 50.771605 Tz (the ) Tj 1.147222 0 Td 47.314815 Tz (steps ) Tj 1.652778 0 Td 55.092593 Tz (of ) Tj 1.011111 0 Td 57.523148 Tz (admixing ) Tj 2.955556 0 Td 56.712963 Tz (an ) Tj 0.913889 0 Td 48.206019 Tz (alginate ) Tj 2.508333 0 Td 48.611111 Tz (solution ) Tj 2.566667 0 Td 53.472222 Tz (with ) Tj 1.516667 0 Td 55.092593 Tz (a ) Tj 0.544444 0 Td 44.722222 Tz (sufficient ) Tj 51.428571 0 0 51.428571 0 1853.8 Tm 6.358333 0 Td 61.033951 Tz (amount ) Tj 2.333333 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 58.333333 Tz (a ) Tj 0.486111 0 Td 48.971193 Tz (bioactive ) Tj 2.8 0 Td 52.5 Tz (agent ) Tj 1.691667 0 Td 60.493827 Tz (and ) Tj 1.225 0 Td 45.37037 Tz (at ) Tj 0.680556 0 Td 44.722222 Tz (least ) Tj 1.477778 0 Td 57.253086 Tz (one ) Tj 1.225 0 Td 53.703704 Tz (calcium ) Tj 2.430556 0 Td 39.537037 Tz (salt, ) Tj 1.341667 0 Td 45.37037 Tz (in ) Tj 0.719444 0 Td 55.092593 Tz (a ) Tj 51.428571 0 0 51.428571 0 1814.431302 Tm 6.377778 0 Td 44.722222 Tz (Sufficient ) Tj 2.838889 0 Td 61.574074 Tz (amount ) Tj 2.372222 0 Td 46.990741 Tz (to ) Tj 0.738889 0 Td 52.5 Tz (allow ) Tj 1.808333 0 Td 48.611111 Tz (for ) Tj 1.030556 0 Td 52.314815 Tz (polymerization ) Tj 4.569444 0 Td 46.018519 Tz (after ) Tj 1.536111 0 Td 47.890947 Tz (injection ) Tj 51.428571 0 0 51.428571 0 1772.701299 Tm 6.358333 0 Td 56.712963 Tz (of ) Tj 0.738889 0 Td 48.611111 Tz (said ) Tj 1.263889 0 Td 50.378788 Tz (biomaterial ) Tj 3.461111 0 Td 55.452675 Tz (composite ) Tj 3.091667 0 Td 59.41358 Tz (and ) Tj 1.186111 0 Td 46.990741 Tz (to ) Tj 0.7 0 Td 57.523148 Tz (form ) Tj 1.516667 0 Td 58.333333 Tz (a ) Tj 0.466667 0 Td 53.472222 Tz (hydrogel ) Tj 2.702778 0 Td 56.481481 Tz (wherein ) Tj 51.428571 0 0 51.428571 0 1731.141743 Tm 6.338889 0 Td 50.771605 Tz (the ) Tj 1.205556 0 Td 54.166667 Tz (calcium ) Tj 2.605556 0 Td 42.12963 Tz (salt ) Tj 1.302778 0 Td 38.888889 Tz (is ) Tj 0.777778 0 Td 55.092593 Tz (a ) Tj 0.641667 0 Td 56.17284 Tz (nonporous ) Tj 3.325 0 Td 52.572016 Tz (aggregate ) Tj 3.130556 0 Td 58.333333 Tz (of ) Tj 1.108333 0 Td 46.990741 Tz (3-tricalcium ) Tj 51.428571 0 0 51.428571 0 1691.585608 Tm 6.358333 0 Td 54.73251 Tz (phosphate ) Tj 3.227778 0 Td 58.333333 Tz (and ) Tj 1.322222 0 Td 48.611111 Tz (said ) Tj 1.4 0 Td 50.378788 Tz (biomaterial ) Tj 3.616667 0 Td 55.452675 Tz (composite ) Tj 3.247222 0 Td 38.888889 Tz (is ) Tj 0.758333 0 Td 56.17284 Tz (comprised ) Tj 3.305556 0 Td 56.712963 Tz (of ) Tj 52.857143 0 0 52.857143 0 1646.37037 Tm 6.167568 0 Td 56.756757 Tz (between ) Tj 2.667568 0 Td 55.18018 Tz (10 ) Tj 0.908108 0 Td 42.567568 Tz (to ) Tj 0.756757 0 Td 59.90991 Tz (20 ) Tj 0.945946 0 Td 50 Tz (percent ) Tj 2.308108 0 Td 58.333333 Tz (by ) Tj 0.983784 0 Td 57.807808 Tz (volume ) Tj 2.289189 0 Td 58.333333 Tz (of ) Tj 0.964865 0 Td 46.771772 Tz (B-tricalcium ) Tj 3.594595 0 Td 66.216216 Tz (phos ) Tj 51.428571 0 0 51.428571 0 1611 Tm 6.358333 0 Td 48.070988 Tz (phate. ) Tj 51.428571 0 0 51.428571 0 1562.962073 Tm 7.097222 0 Td 49.691358 Tz (30. ) Tj 1.030556 0 Td 62.654321 Tz (The ) Tj 1.263889 0 Td 51.388889 Tz (process ) Tj 2.313889 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 53.148148 Tz (claim ) Tj 1.75 0 Td 59.953704 Tz (29 ) Tj 0.894444 0 Td 55.092593 Tz (wherein ) Tj 2.469444 0 Td 48.611111 Tz (said ) Tj 1.302778 0 Td 48.971193 Tz (bioactive ) Tj 2.741667 0 Td 53.796296 Tz (agent ) Tj 1.730556 0 Td 40.509259 Tz (is ) Tj 51.428571 0 0 51.428571 0 1524.388013 Tm 6.358333 0 Td 53.148148 Tz (angiogenic ) Tj 3.363889 0 Td 59.41358 Tz (and ) Tj 1.225 0 Td 50.771605 Tz (the ) Tj 1.088889 0 Td 42.12963 Tz (site ) Tj 1.205556 0 Td 38.888889 Tz (is ) Tj 0.641667 0 Td 51.041667 Tz (adjacent ) Tj 2.605556 0 Td 46.990741 Tz (to ) Tj 0.758333 0 Td 44.398148 Tz (cartilage. ) Tj 51.428571 0 0 51.428571 0 1479.962073 Tm 7.097222 0 Td 49.691358 Tz (31. ) Tj 1.030556 0 Td 62.654321 Tz (The ) Tj 1.263889 0 Td 51.388889 Tz (process ) Tj 2.313889 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 53.148148 Tz (claim ) Tj 1.75 0 Td 59.953704 Tz (29 ) Tj 0.894444 0 Td 55.092593 Tz (wherein ) Tj 2.469444 0 Td 48.611111 Tz (said ) Tj 1.302778 0 Td 48.971193 Tz (bioactive ) Tj 2.741667 0 Td 53.796296 Tz (agent ) Tj 1.730556 0 Td 40.509259 Tz (is ) Tj 51.428571 0 0 51.428571 0 1437.953728 Tm 6.358333 0 Td 55.092593 Tz (a ) Tj 0.544444 0 Td 56.17284 Tz (growth ) Tj 2.255556 0 Td 47.530864 Tz (factor ) Tj 1.866667 0 Td 60.493827 Tz (and ) Tj 1.263889 0 Td 51.851852 Tz (the ) Tj 1.127778 0 Td 42.12963 Tz (site ) Tj 1.225 0 Td 38.888889 Tz (is ) Tj 0.719444 0 Td 48.611111 Tz (selected ) Tj 2.527778 0 Td 58.333333 Tz (from ) Tj 1.633333 0 Td 48.611111 Tz (the ) Tj 1.108333 0 Td 55.740741 Tz (group ) Tj 1.905556 0 Td 71.296296 Tz (con ) Tj 51.428571 0 0 51.428571 0 1396.040214 Tm 6.377778 0 Td 44.907407 Tz (sisting ) Tj 2.061111 0 Td 55.092593 Tz (of ) Tj 0.972222 0 Td 59.953704 Tz (bone ) Tj 1.613889 0 Td 45.37037 Tz (fracture, ) Tj 2.605556 0 Td 59.953704 Tz (bone ) Tj 1.613889 0 Td 53.472222 Tz (Void ) Tj 1.458333 0 Td 59.41358 Tz (and ) Tj 1.244444 0 Td 59.143519 Tz (bone ) Tj 1.633333 0 Td 52.932099 Tz (nonunion. ) Tj 30 0 0 30 0 2547 Tm 41.666667 0 Td 75 Tz (10 ) Tj 30 0 0 30 0 2339 Tm 41.666667 0 Td 72.222222 Tz (15 ) Tj 30 0 0 30 0 1923 Tm 41.6 0 Td 77.777778 Tz (25 ) Tj 30 0 0 30 0 1716 Tm 41.6 0 Td 80.555556 Tz (30 ) Tj 30 0 0 30 0 1507 Tm 41.6 0 Td 77.777778 Tz (35 ) Tj 52.857143 0 0 52.857143 0 2968 Tm 32.635135 0 Td 75.675676 Tz (32 ) Tj 51.428571 0 0 51.428571 0 2914.191489 Tm 26.016667 0 Td 49.691358 Tz (32. ) Tj 1.127778 0 Td 62.654321 Tz (The ) Tj 1.341667 0 Td 51.851852 Tz (process ) Tj 2.391667 0 Td 61.574074 Tz (of ) Tj 0.855556 0 Td 53.148148 Tz (claim ) Tj 1.827778 0 Td 59.953704 Tz (29 ) Tj 0.952778 0 Td 55.555556 Tz (wherein ) Tj 2.566667 0 Td 49.421296 Tz (said ) Tj 1.4 0 Td 42.12963 Tz (site ) Tj 1.225 0 Td 42.12963 Tz (is ) Tj 0.738889 0 Td 48.611111 Tz (selected ) Tj 51.428571 0 0 51.428571 0 2874.39 Tm 25.297222 0 Td 58.333333 Tz (from ) Tj 1.691667 0 Td 49.691358 Tz (the ) Tj 1.166667 0 Td 56.388889 Tz (group ) Tj 2.002778 0 Td 49.259259 Tz (consisting ) Tj 3.227778 0 Td 55.092593 Tz (of ) Tj 1.088889 0 Td 51.851852 Tz (Subchondral, ) Tj 4.044444 0 Td 50.231481 Tz (osteochondral, ) Tj 51.428571 0 0 51.428571 0 2832.548837 Tm 25.297222 0 Td 50.87963 Tz (cancellous ) Tj 3.247222 0 Td 53.148148 Tz (bone, ) Tj 1.788889 0 Td 58.333333 Tz (and ) Tj 1.225 0 Td 44.398148 Tz (cartilage. ) Tj 51.428571 0 0 51.428571 0 2789.972187 Tm 26.016667 0 Td 49.691358 Tz (33. ) Tj 1.030556 0 Td 63.734568 Tz (The ) Tj 1.263889 0 Td 51.851852 Tz (process ) Tj 2.333333 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 53.148148 Tz (claim ) Tj 1.769444 0 Td 58.333333 Tz (29 ) Tj 0.855556 0 Td 55.092593 Tz (wherein ) Tj 2.488889 0 Td 47.800926 Tz (said ) Tj 1.283333 0 Td 48.971193 Tz (bioactive ) Tj 2.761111 0 Td 53.796296 Tz (agent ) Tj 1.730556 0 Td 40.509259 Tz (is ) Tj 51.428571 0 0 51.428571 0 2750.392405 Tm 25.297222 0 Td 51.388889 Tz (derived ) Tj 2.333333 0 Td 57.523148 Tz (from ) Tj 1.575 0 Td 43.425926 Tz (platelets. ) Tj 51.428571 0 0 51.428571 0 2706.969697 Tm 26.016667 0 Td 49.691358 Tz (34. ) Tj 1.030556 0 Td 63.734568 Tz (The ) Tj 1.263889 0 Td 51.851852 Tz (process ) Tj 2.333333 0 Td 55.092593 Tz (of ) Tj 0.738889 0 Td 53.148148 Tz (claim ) Tj 1.730556 0 Td 59.953704 Tz (33 ) Tj 0.894444 0 Td 55.092593 Tz (wherein ) Tj 2.488889 0 Td 47.800926 Tz (said ) Tj 1.283333 0 Td 48.971193 Tz (bioactive ) Tj 2.761111 0 Td 53.796296 Tz (agent ) Tj 1.730556 0 Td 40.509259 Tz (is ) Tj 51.428571 0 0 51.428571 0 2667.235849 Tm 25.297222 0 Td 44.965278 Tz (platelet ) Tj 2.236111 0 Td 47.890947 Tz (releasate ) Tj 2.663889 0 Td 60.493827 Tz (and ) Tj 1.205556 0 Td 49.691358 Tz (the ) Tj 1.011111 0 Td 48.971193 Tz (bioactive ) Tj 2.741667 0 Td 53.148148 Tz (agent ) Tj 1.730556 0 Td 54.012346 Tz (comprises ) Tj 3.033333 0 Td 45.37037 Tz (at ) Tj 0.680556 0 Td 44.074074 Tz (least ) Tj 1.438889 0 Td 61.574074 Tz (50 ) Tj 51.428571 0 0 51.428571 0 2626.786749 Tm 25.297222 0 Td 51.388889 Tz (percent ) Tj 2.294444 0 Td 61.574074 Tz (by ) Tj 0.952778 0 Td 59.953704 Tz (volume ) Tj 2.333333 0 Td 59.953704 Tz (of ) Tj 0.797222 0 Td 49.691358 Tz (the ) Tj 1.069444 0 Td 50.411523 Tz (hydrogel. ) Tj 51.428571 0 0 51.428571 0 2584.919463 Tm 26.016667 0 Td 49.691358 Tz (35. ) Tj 1.166667 0 Td 62.654321 Tz (The ) Tj 1.380556 0 Td 51.851852 Tz (process ) Tj 2.45 0 Td 59.953704 Tz (of ) Tj 0.875 0 Td 53.148148 Tz (claim ) Tj 1.866667 0 Td 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Tz (a ) Tj 0.525 0 Td 47.746914 Tz (therapeutically ) Tj 4.530556 0 Td 46.450617 Tz (effective ) Tj 51.428571 0 0 51.428571 0 2211.141414 Tm 25.297222 0 Td 61.033951 Tz (amount ) Tj 2.469444 0 Td 56.712963 Tz (of ) Tj 0.836111 0 Td 58.333333 Tz (a ) Tj 0.602778 0 Td 54.503367 Tz (composition ) Tj 3.869444 0 Td 52.441077 Tz (comprising: ) Tj 3.733333 0 Td 56.712963 Tz (an ) Tj 0.972222 0 Td 47.314815 Tz (injectable ) Tj 3.111111 0 Td 45.37037 Tz (in ) Tj 0.816667 0 Td 43.75 Tz (situ ) Tj 51.428571 0 0 51.428571 0 2170.990338 Tm 25.297222 0 Td 51.851852 Tz (curing ) Tj 2.080556 0 Td 53.877315 Tz (hydrogel ) Tj 2.819444 0 Td 50.378788 Tz (biomaterial ) Tj 3.577778 0 Td 55.452675 Tz (composite ) Tj 3.208333 0 Td 54.768519 Tz (comprising ) Tj 3.5 0 Td 59.953704 Tz (an ) Tj 0.952778 0 Td 55.092593 Tz (algi ) Tj 51.428571 0 0 51.428571 0 2125.962241 Tm 25.297222 0 Td 51.041667 Tz (nate ) Tj 1.419444 0 Td 48.611111 Tz (gel ) Tj 1.069444 0 Td 55.092593 Tz (having ) Tj 2.158333 0 Td 62.789352 Tz 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